Addgene pVH016 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pVH016 Sequencing Result

Map View

800 600 400 200 End (912) SacI (907) Eco53kI (905) SapI - BspQI (857) AcuI (719) BsrFI (705) StyI (698) EaeI (695) BspEI - BsaWI (642) EciI (624) BsaAI (554) MluI - AflIII (542) BbsI (463) DraIII (361) PvuII (337) BclI * (327) BpmI (188) BsrGI (157) MslI (137) BspHI * (117) HindIII (76) ApaI - BaeGI - Bme1580I (74) PspOMI (70) HaeII (35) AccI (26) SalI (25) Start (0) 6xHis Myc 3xFLAG superfolder GFP pVH016 912 bp

Sequence View

HaeIISalIAccIStart (0) 5ʹ 3ʹ 60 * 5 1 HHHHHH (in frame with Myc) DVASN 10 5 LDEESIL 6xHis Myc MS a a c t c a a t g a t g a t g a t g a t g a t g g t c g a c g g c g c t a t t c a g a t c c t c t t c t g a g a t g a g t t g a g t t a c t a c t a c t a c t a c t a c c a g c t g c c g c g a t a a g t c t a g g a g a a g a c t c t a c t c

BspHI*PspOMIBme1580IBaeGIApaIHindIII 120 1 KQE * 20 15 KDDDDKYDIDH Myc 3xFLAG FCSGPSFSLLVIVILVVDVM *KDDDDKYDIDH t t t t t g t t c g g g c c c a a g c t t c t c a c t a c t t g t c a t c g t c a t c c t t g t a g t c g a t g t c a t a a a a a c a a g c c c g g g t t c g a a g a g t g a t g a a c a g t a g c a g t a g g a a c a t c a g c t a c a g t a

BsrGIMslI 180 10 5 1 DKYDGDHDKYD 235 230 KYLEDMGHT 3xFLAG superfolder GFP ILVITVMVLVVFVQFIHTMR DKYDGDHDKYDKYLEDMGHT g a t c c t t g t a a t c a c c g t c a t g g t c c t t g t a g t c t t t g t a c a g t t c a t c c a t a c c a t g c g c t a g g a a c a t t a g t g g c a g t a c c a g g a a c a t c a g a a a c a t g t c a a g t a g g t a t g g t a c g c

BpmI 240 225 220 215 210 IGAATVFELLVMHDRKENPD superfolder GFP DARCGYELQQNHMIAFLVRI IGAATVFELLVMHDRKENPD t g a t g c c c g c t g c g g t t a c g a a c t c c a g c a g a a c c a t a t g a t c g c g t t t c t c g t t c g g a t a c t a c g g g c g a c g c c a a t g c t t g a g g t c g t c t t g g t a t a c t a g c g c a a a g a g c a a g c c t a

300 205 200 195 190 KSLVSQTSLYHNDPLLVPGD superfolder GFP FRQNALRAQIVIVWQQNRTI KSLVSQTSLYHNDPLLVPGD c t t t a g a c a g a a c g c t t t g c g t g c t c a g a t a g t g a t t g t c t g g c a g c a g a a c a g g a c c a t g a a a t c t g t c t t g c g a a a c g c a c g a g t c t a t c a c t a a c a g a c c g t c g t c t t g t c c t g g t a

PvuIIBclI* 360 185 180 175 170 GIPTNQQYHDALQVSGDEVN superfolder GFP TDWSVLLVVISQLHAAILHV GIPTNQQYHDALQVSGDEVN c a c c g a t t g g a g t g t t t t g c t g g t a g t g a t c a g c c a g c t g c a c g c t g c c a t c c t c c a c g t g t g g c t a a c c t c a c a a a a c g a c c a t c a c t a g t c g g t c g a c g t g c g a c g g t a g g a g g t g c a

DraIII 420 165 160 155 150 HRIKFNAKIGNKQKDATIYV superfolder GFP VANFKIRFNAIFLFIGGDVN HRIKFNAKIGNKQKDATIYV t g t g g c g a a t t t t a a a a t t c g c t t t a a t g c c a t t t t t t t g t t t a t c g g c g g t g a t g t a a a a c a c c g c t t a a a a t t t t a a g c g a a a t t a c g g t a a a a a a a c a a a t a g c c g c c a c t a c a t t t

BbsI 480 145 140 135 130 NHSNFNYELKHGLINGDEKF superfolder GFP IVAVKIVFQLMAQDIAVFFK NHSNFNYELKHGLINGDEKF c a t t g t g g c t g t t a a a a t t g t a t t c c a g c t t a t g g c c c a g g a t a t t g c c g t c t t c t t t a a g t a a c a c c g a c a a t t t t a a c a t a a g g t c g a a t a c c g g g t c c t a t a a c g g c a g a a g a a a t t

540 125 120 115 110 DIGKLEIRNVLTDGEFKVEA superfolder GFP VNAFQLNAVYQGIAFKFHFR DIGKLEIRNVLTDGEFKVEA a g t c a a t g c c t t t c a g c t c a a t g c g g t t t a c c a g g g t a t c g c c t t c a a a t t t c a c t t c c g t c a g t t a c g g a a a g t c g a g t t a c g c c a a a t g g t c c c a t a g c g g a a g t t t a a a g t g a a g g c

BsaAIAflIIIMluI 600 105 100 95 90 RTKYTGDDKFSITREQVYGE superfolder GFP TRFVRAVILKGNRAFLHIAF RTKYTGDDKFSITREQVYGE c a c g c g t t t t g t a c g t g c c g t c a t c c t t a a a g g a a a t c g t g c g t t c c t g c a c a t a g c c t t g t g c g c a a a a c a t g c a c g g c a g t a g g a a t t t c c t t t a g c a c g c a a g g a c g t g t a t c g g a a

BsaWIBspEIEciI 660 85 80 75 70 PMASKFFDHQKMHDPYRAFC superfolder GFP RHGGLEEVMLLHMVRITSKA PMASKFFDHQKMHDPYRAFC c c g g c a t g g c g g a c t t g a a g a a g t c a t g c t g c t t c a t a t g g t c c g g a t a a c g a g c a a a g c g g c c g t a c c g c c t g a a c t t c t t c a g t a c g a c g a a g t a t a c c a g g c c t a t t g c t c g t t t c g

AcuIBsrFIEaeIStyI 720 65 60 55 50 QVGYTLTTVLTPWPVPLKGT superfolder GFP LNTISQRRYQSRPRNRQFTS QVGYTLTTVLTPWPVPLKGT a c t g a a c a c c a t a a g t c a g c g t c g t t a c c a g a g t c g g c c a a g g a a c c g g c a g t t t a c c a g t g a c t t g t g g t a t t c a g t c g c a g c a a t g g t c t c a g c c g g t t c c t t g g c c g t c a a a t g g t c

780 45 40 35 30 TCIFKLTLKGNTADGEGEGR superfolder GFP STDELQRQFTISCVTFTLAT TCIFKLTLKGNTADGEGEGR t a g t a c a g a t g a a c t t c a g c g t c a g t t t a c c a t t a g t t g c g t c a c c t t c a c c c t c g c c a c a t c a t g t c t a c t t g a a g t c g c a g t c a a a t g g t a a t c a a c g c a g t g g a a g t g g g a g c g g t g

840 25 20 15 10 VSFKHGNVDGDLEVLIPVVG superfolder GFP HGKLMTVDITIQFHQNRDDT VSFKHGNVDGDLEVLIPVVG g c a c g g a a a a c t t a t g a c c g t t g a c a t c a c c a t c c a g t t c c a c c a g a a t a g g g a c g a c a c c g t g c c t t t t g a a t a c t g g c a a c t g t a g t g g t a g g t c a a g g t g g t c t t a t c c c t g c t g t g

BspQISapI 900 5 1 TFLEEGKRM superfolder GFP SEQLFAFTHLILLRPFLTNV TFLEEGKRM c a g t g a a c a g c t c t t c g c c t t t a c g c a t c t a a t c c t c c t g c g c c c g t t t c t t a c g a a t g t g t c a c t t g t c g a g a a g c g g a a a t g c g t a g a t t a g g a g g a c g c g g g c a a a g a a t g c t t a c a

End (912) Eco53kISacI 3ʹ 5ʹ 912 * a t g a g c t c a t a a t a c t c g a g t a t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.