Addgene Hs.KRAS4b G12D - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: Hs.KRAS4b G12D


Map View

HincII - HpaI (2) BbsI (3198) AclI (3125) PvuII (2935) BspQI - SapI (2872) L4440 (2853 .. 2870) PciI (2755) DrdI (2653) pBR322ori-F (2600 .. 2619) BssSI - BssSαI (2582) BciVI (2557) PspFI (2455) BseYI (2451) AlwNI (2346) PaeR7I - PspXI - XhoI (2021) BsaAI (1982) BssHII (1949) NaeI (1876) NgoMIV (1874) BglI (1857) BtgZI (1783) PvuI (1606) M13/pUC Forward (23 .. 45) M13 Forward (37 .. 54) M13 fwd AhdI (50) Eco53kI (67) BanII - SacI (69) RsrII (71) BamHI (78) PsiI (153) BanI (180) BseRI (384) XmnI (460) AccI (559) Bpu10I (574) EcoRI (598) BstBI (674) SphI (854) HindIII (856) BstEII (1385) BclI * (1423) BpmI (1477) MslI (1497) BlpI (1534) Hs.KRAS4b G12D 3260 bp

Sequence View

AhdIHincIIHpaI 60 M13 fwd C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward t t a a c g c t a g c a t g g a t g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t c t t a a a a a t t g c g a t c g t a c c t a c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a g a a t t t

BamHIEco53kIBanIISacIRsrII 120 attL1 a t t c g a g c t c g g t c c g a g g a t c c g a a a t a a t g a t t t t a t t t t g a c t g a t a g t g a c c t g t t t a a g c t c g a g c c a g g c t c c t a g g c t t t a t t a c t a a a a t a a a a c t g a c t a t c a c t g g a c a a

PsiI 180 attL1 MPTLYKKV c g t t g c a a c a a a t t g a t a a g c a a t g c t t t t t t a t a a t g c c a a c t t t g t a c a a a a a a g t t g g c a a c g t t g t t t a a c t a t t c g t t a c g a a a a a a t a t t a c g g t t g a a a c a t g t t t t t t c a a c

BanI 240 1 5 10 15 MTEYKLVVVGADGVGKSA attL1 K-Ras 1 5 10 MTEYKLVVVGA D 15 GVGKSA K-Ras (G12V) GTMTEYKLVVVGADGVGKSA g c a c c a t g a c t g a a t a t a a a c t t g t g g t a g t t g g a g c t g a t g g c g t a g g c a a g a g t g c c t c g t g g t a c t g a c t t a t a t t t g a a c a c c a t c a a c c t c g a c t a c c g c a t c c g t t c t c a c g g a

300 20 25 30 35 LTIQLIQNHFVDEYDPTIED K-Ras 20 25 30 35 LTIQLIQNHFVDEYDPTIED K-Ras (G12V) LTIQLIQNHFVDEYDPTIED t g a c g a t a c a g c t a a t t c a g a a t c a t t t t g t g g a c g a a t a t g a t c c a a c a a t a g a g g a t t a c t g c t a t g t c g a t t a a g t c t t a g t a a a a c a c c t g c t t a t a c t a g g t t g t t a t c t c c t a a

360 40 45 50 55 SYRKQVVIDGETCLLDILDT K-Ras 40 45 50 55 SYRKQVVIDGETCLLDILDT K-Ras (G12V) SYRKQVVIDGETCLLDILDT c c t a c a g g a a g c a a g t a g t a a t t g a t g g a g a a a c c t g t c t c t t g g a t a t t c t c g a c a c a g g g a t g t c c t t c g t t c a t c a t t a a c t a c c t c t t t g g a c a g a g a a c c t a t a a g a g c t g t g t c

BseRI 420 60 65 70 75 AGQEEYSAMRDQYMRTGEGF K-Ras 60 65 70 75 AGQEEYSAMRDQYMRTGEGF K-Ras (G12V) AGQEEYSAMRDQYMRTGEGF c a g g t c a a g a g g a g t a c a g t g c a a t g a g g g a c c a g t a c a t g a g g a c t g g g g a g g g c t t t c g t c c a g t t c t c c t c a t g t c a c g t t a c t c c c t g g t c a t g t a c t c c t g a c c c c t c c c g a a a g

XmnI 480 80 85 90 95 LCVFAINNTKSFEDIHHYRE K-Ras 80 85 90 95 LCVFAINNTKSFEDIHHYRE K-Ras (G12V) LCVFAINNTKSFEDIHHYRE t t t g t g t a t t t g c c a t a a a t a a t a c t a a a t c a t t t g a a g a t a t t c a c c a t t a t a g a g a a c a a a c a c a t a a a c g g t a t t t a t t a t g a t t t a g t a a a c t t c t a t a a g t g g t a a t a t c t c t t g

540 100 105 110 115 QIKRVKDSEDVPMVLVGNKC K-Ras 100 105 110 115 QIKRVKDSEDVPMVLVGNKC K-Ras (G12V) QIKRVKDSEDVPMVLVGNKC a a a t t a a a a g a g t t a a g g a c t c t g a a g a t g t a c c t a t g g t c c t a g t a g g a a a t a a a t g t g t t t a a t t t t c t c a a t t c c t g a g a c t t c t a c a t g g a t a c c a g g a t c a t c c t t t a t t t a c a c

EcoRIBpu10IAccI 600 120 125 130 135 DLPSRTVDTKQAQDLARSYG K-Ras 120 125 130 135 DLPSRTVDTKQAQDLARSYG K-Ras (G12V) DLPSRTVDTKQAQDLARSYG a t t t g c c t t c t a g a a c a g t a g a c a c a a a a c a g g c t c a g g a c t t a g c a a g a a g t t a t g g a a t a a a c g g a a g a t c t t g t c a t c t g t g t t t t g t c c g a g t c c t g a a t c g t t c t t c a a t a c c t t

660 140 145 150 155 IPFIETSAKTRQGVDDAFYT K-Ras 140 145 150 155 IPFIETSAKTRQGVDDAFYT K-Ras (G12V) IPFIETSAKTRQGVDDAFYT t t c c t t t t a t t g a a a c a t c a g c a a a g a c a a g a c a g g g t g t t g a t g a t g c c t t c t a t a c a t a a g g a a a a t a a c t t t g t a g t c g t t t c t g t t c t g t c c c a c a a c t a c t a c g g a a g a t a t g t a

BstBI 720 160 165 170 175 LVREIRKHKEKMSKDGKKKK K-Ras 160 165 170 175 LVREIRKHKEKMSKDGKKKK K-Ras (G12V) LVREIRKHKEKMSKDGKKKK t a g t t c g a g a a a t t c g a a a a c a t a a a g a a a a g a t g a g c a a a g a t g g t a a a a a g a a g a a a a a t c a a g c t c t t t a a g c t t t t g t a t t t c t t t t c t a c t c g t t t c t a c c a t t t t t c t t c t t t t

780 180 185 KKSKTKCVIM* K-Ras 180 185 KKSKTKCVIM* K-Ras (G12V) attL2 KKSKTKCVIM* a g a a g t c a a a g a c a a a g t g t g t a a t t a t g t a a t c a a c t t t c t t g t a c a a a g t t g g c a t t a t c t t c a g t t t c t g t t t c a c a c a t t a a t a c a t t a g t t g a a a g a a c a t g t t t c a a c c g t a a t

840 attL2 t a g g a a a g c a t t g c t t a t c a a t t t g t t g c a a c g a a c a g g t c a c t a t c a g t c a a a a t a a a a a t c c t t t c g t a a c g a a t a g t t a a a c a a c g t t g c t t g t c c a g t g a t a g t c a g t t t t a t t t t

SphIHindIII 900 attL2 t c a t t a t t t g c a t g c a a g c t t a g a t c c t c t a g a c c a g c c a g g a c a g a a a t g c c t c g a c t t a g t a a t a a a c g t a c g t t c g a a t c t a g g a g a t c t g g t c g g t c c t g t c t t t a c g g a g c t g a a

960 c g c t g c t a c c c a a g g t t g c c g g g t g a c g c a c a c c g t g g a a a c g g a t g a a g g c a c g a a c c c g c g a c g a t g g g t t c c a a c g g c c c a c t g c g t g t g g c a c c t t t g c c t a c t t c c g t g c t t g g g

1020 MRSR a g t g g a c a t a a g c c t g t t c g g t t c g t a a g c t g t a a t g c a a g t a g c g t a t g c g c t c a c g c a t c a c c t g t a t t c g g a c a a g c c a a g c a t t c g a c a t t a c g t t c a t c g c a t a c g c g a g t g c g t

1080 NWSRTLTERSGGNGAVAVFM a c t g g t c c a g a a c c t t g a c c g a a c g c a g c g g t g g t a a c g g c g c a g t g g c g g t t t t c a t g g t g a c c a g g t c t t g g a a c t g g c t t g c g t c g c c a c c a t t g c c g c g t c a c c g c c a a a a g t a c c

1140 ACYDCFFGVQSMPRASKQQA c t t g t t a t g a c t g t t t t t t t g g g g t a c a g t c t a t g c c t c g g g c a t c c a a g c a g c a a g c g c g a a c a a t a c t g a c a a a a a a a c c c c a t g t c a g a t a c g g a g c c c g t a g g t t c g t c g t t c g c g

1200 RYAVGRCLMLWSSNDVTQQG g t t a c g c c g t g g g t c g a t g t t t g a t g t t a t g g a g c a g c a a c g a t g t t a c g c a g c a g g g c a c a a t g c g g c a c c c a g c t a c a a a c t a c a a t a c c t c g t c g t t g c t a c a a t g c g t c g t c c c g t

1260 1 5 10 MREAVIAEVST SmR SRPKTKLNIMREAVIAEVST g t c g c c c t a a a a c a a a g t t a a a c a t t a t g a g g g a a g c g g t g a t c g c c g a a g t a t c g a c t c c a g c g g g a t t t t g t t t c a a t t t g t a a t a c t c c c t t c g c c a c t a g c g g c t t c a t a g c t g a g

1320 15 20 25 30 QLSEVVGVIERHLEPTLLAV SmR QLSEVVGVIERHLEPTLLAV a a c t a t c a g a g g t a g t t g g c g t c a t c g a g c g c c a t c t c g a a c c g a c g t t g c t g g c c g t a c t t g a t a g t c t c c a t c a a c c g c a g t a g c t c g c g g t a g a g c t t g g c t g c a a c g a c c g g c a t g

1380 35 40 45 50 HLYGSAVDGGLKPHSDIDLL SmR HLYGSAVDGGLKPHSDIDLL a t t t g t a c g g c t c c g c a g t g g a t g g c g g c c t g a a g c c a c a c a g t g a t a t t g a t t t g c t g g t a a a c a t g c c g a g g c g t c a c c t a c c g c c g g a c t t c g g t g t g t c a c t a t a a c t a a a c g a c c

BclI*BstEII 1440 55 60 65 70 VTVTVRLDETTRRALINDLL SmR VTVTVRLDETTRRALINDLL t t a c g g t g a c c g t a a g g c t t g a t g a a a c a a c g c g g c g a g c t t t g a t c a a c g a c c t t t t g g a a t g c c a c t g g c a t t c c g a a c t a c t t t g t t g c g c c g c t c g a a a c t a g t t g c t g g a a a a c c

MslIBpmI 1500 75 80 85 90 ETSASPGESEILRAVEVTIV SmR ETSASPGESEILRAVEVTIV a a a c t t c g g c t t c c c c t g g a g a g a g c g a g a t t c t c c g c g c t g t a g a a g t c a c c a t t g t t g t t t g a a g c c g a a g g g g a c c t c t c t c g c t c t a a g a g g c g c g a c a t c t t c a g t g g t a a c a a c

BlpI 1560 95 100 105 110 VHDDIIPWRYPAKRELQFGE SmR VHDDIIPWRYPAKRELQFGE t g c a c g a c g a c a t c a t t c c g t g g c g t t a t c c a g c t a a g c g c g a a c t g c a a t t t g g a g a a t a c g t g c t g c t g t a g t a a g g c a c c g c a a t a g g t c g a t t c g c g c t t g a c g t t a a a c c t c t t a

PvuI 1620 115 120 125 130 WQRNDILAGIFEPATIDIDL SmR WQRNDILAGIFEPATIDIDL g g c a g c g c a a t g a c a t t c t t g c a g g t a t c t t c g a g c c a g c c a c g a t c g a c a t t g a t c t g g c c g t c g c g t t a c t g t a a g a a c g t c c a t a g a a g c t c g g t c g g t g c t a g c t g t a a c t a g a c c

1680 135 140 145 150 AILLTKAREHSVALVGPAAE SmR AILLTKAREHSVALVGPAAE c t a t c t t g c t g a c a a a a g c a a g a g a a c a t a g c g t t g c c t t g g t a g g t c c a g c g g c g g a g g g a t a g a a c g a c t g t t t t c g t t c t c t t g t a t c g c a a c g g a a c c a t c c a g g t c g c c g c c t c c

1740 155 160 165 170 ELFDPVPEQDLFEALNETLT SmR ELFDPVPEQDLFEALNETLT a a c t c t t t g a t c c g g t t c c t g a a c a g g a t c t a t t t g a g g c g c t a a a t g a a a c c t t a a c g c t t g a g a a a c t a g g c c a a g g a c t t g t c c t a g a t a a a c t c c g c g a t t t a c t t t g g a a t t g c g

BtgZI 1800 175 180 185 190 LWNSPPDWAGDERNVVLTLS SmR LWNSPPDWAGDERNVVLTLS t a t g g a a c t c g c c g c c c g a c t g g g c t g g c g a t g a g c g a a a t g t a g t g c t t a c g t t g t c c c a t a c c t t g a g c g g c g g g c t g a c c c g a c c g c t a c t c g c t t t a c a t c a c g a a t g c a a c a g g g

BglI 1860 195 200 205 210 RIWYSAVTGKIAPKDVAADW SmR RIWYSAVTGKIAPKDVAADW g c a t t t g g t a c a g c g c a g t a a c c g g c a a a a t c g c g c c g a a g g a t g t c g c t g c c g a c t g g g c g t a a a c c a t g t c g c g t c a t t g g c c g t t t t a g c g c g g c t t c c t a c a g c g a c g g c t g a c c c

NgoMIVNaeI 1920 215 220 225 230 AMERLPAQYQPVILEARQAY SmR AMERLPAQYQPVILEARQAY c a a t g g a g c g c c t g c c g g c c c a g t a t c a g c c c g t c a t a c t t g a a g c t a g a c a g g c t t a t c g t t a c c t c g c g g a c g g c c g g g t c a t a g t c g g g c a g t a t g a a c t t c g a t c t g t c c g a a t a g

BssHII 1980 235 240 245 250 LGQEEDRLASRADQLEEFVH SmR LGQEEDRLASRADQLEEFVH t t g g a c a a g a a g a a g a t c g c t t g g c c t c g c g c g c a g a t c a g t t g g a a g a a t t t g t c c a c t a a c c t g t t c t t c t t c t a g c g a a c c g g a g c g c g c g t c t a g t c a a c c t t c t t a a a c a g g t g a

PaeR7IPspXIXhoIBsaAI 2040 255 260 YVKGEITKVVGK* SmR YVKGEITKVVGK* a c g t g a a a g g c g a g a t c a c c a a g g t a g t c g g c a a a t a a c c c t c g a g c c a c c c a t g a c c a a t g c a c t t t c c g c t c t a g t g g t t c c a t c a g c c g t t t a t t g g g a g c t c g g t g g g t a c t g g t t

2100 a a t c c c t t a a c g t g a g t t a c g c g t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a t t a g g g a a t t g c a c t c a a t g c g c a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t

2160 ori a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g

2220 ori c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c

2280 ori t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c

2340 ori g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g

AlwNI 2400 ori c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a

BseYIPspFI 2460 ori t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c

2520 ori a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c a t t g a g a a a g c g c c a c g c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g t a a c t c t t t c g c g g t g c g

BciVI 2580 ori t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g

BssSαIBssSI 2640 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g

DrdI 2700 ori a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t

PciI 2760 a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a

2820 t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c

BspQISapI 2880 A G C G A G T C A G T G A G C G A G L4440 a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c

PvuII 2940 a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g

3000 a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t a c g c g t a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a t g c g c a t g g c

3060 rrnB T2 terminator c t a g c c a g g a a g a g t t t g t a g a a a c g c a a a a a g g c c a t c c g t c a g g a t g g c c t t c t g c t t g a t c g g t c c t t c t c a a a c a t c t t t g c g t t t t t c c g g t a g g c a g t c c t a c c g g a a g a c g a a

3120 a g t t t g a t g c c t g g c a g t t t a t g g c g g g c g t c c t g c c c g c c a c c c t c c g g g c c g t t g c t t t c a a a c t a c g g a c c g t c a a a t a c c g c c c g c a g g a c g g g c g g t g g g a g g c c c g g c a a c g a a

AclI 3180 rrnB T1 terminator c a c a a c g t t c a a a t c c g c t c c c g g c g g a t t t g t c c t a c t c a g g a g a g c g t t c a c c g a c a a g t g t t g c a a g t t t a g g c g a g g g c c g c c t a a a c a g g a t g a g t c c t c t c g c a a g t g g c t g t t

BbsI 3240 rrnB T1 terminator a c a a c a g a t a a a a c g a a a g g c c c a g t c t t c c g a c t g a g c c t t t c g t t t t a t t t g a t g c c t t g t t g t c t a t t t t g c t t t c c g g g t c a g a a g g c t g a c t c g g a a a g c a a a a t a a a c t a c g g a

3260 g g c a g t t c c c t a c t c t c g c g c c g t c a a g g g a t g a g a g c g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.