Addgene EGFP gRNA (BRDN0000562805) Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: EGFP gRNA (BRDN0000562805) Sequencing Result


Map View

600 400 200 End (779) BtsI - BtsαI (775) MmeI (770) AleI - MslI (768) StyI (758) BsmI (753) SfcI (475) AvaI - BsoBI (414) KflI - PpuMI (408) hU6-F (383 .. 403) AseI (330) BsaAI (293) LKO.1 5' (213 .. 232) NdeI (222) DraIII (155) BaeGI - Bsp1286I - Bme1580I (151) MflI * - BglII - BstYI (31) SmlI (27) BpuEI (12) Start (0) gRNA scaffold U6 promoter gp41 peptide EGFP gRNA (BRDN0000562805) 779 bp

Sequence View

BstYIBglIIMflI*SmlIBpuEIStart (0) 60 gRNA scaffold a a t t g t g g a t g a a t a c t g c c a t t t g t c t c a a g a t c t a g t t a c g c c a a g c t t a a a a a a g c a t t a a c a c c t a c t t a t g a c g g t a a a c a g a g t t c t a g a t c a a t g c g g t t c g a a t t t t t t c g t

120 gRNA scaffold c c g a c t c g g t g c c a c t t t t t c a a g t t g a t a a c g g a c t a g c c t t a t t t t a a c t t g c t a t t t g g c t g a g c c a c g g t g a a a a a g t t c a a c t a t t g c c t g a t c g g a a t a a a a t t g a a c g a t a a a

Bme1580IBsp1286IBaeGIDraIII 180 gRNA scaffold U6 promoter c t a g c t c t a a a a c g c t c g a c c a g g a t g g g c a c c c g g t g t t t c g t c c t t t c c a c a a g a t a t g a t c g a g a t t t t g c g a g c t g g t c c t a c c c g t g g g c c a c a a a g c a g g a a a g g t g t t c t a t a

NdeI 240 U6 promoter T G C C A T T C G T A T A C T A T C A G LKO.1 5' a t a a a g c c a a g a a a t c g a a a t a c t t t c a a g t t a c g g t a a g c a t a t g a t a g t c c a t t t t a a t a t t t c g g t t c t t t a g c t t t a t g a a a g t t c a a t g c c a t t c g t a t a c t a t c a g g t a a a a t t

BsaAI 300 *TNQ U6 promoter *TNQ a a c a t a a t t t t a a a a c t g c a a a c t a c c c a a g a a a t t a t t a c t t t c t a c g t c a c g t a t t t t t t g t a t t a a a a t t t t g a c g t t t g a t g g g t t c t t t a a t a a t g a a a g a t g c a g t g c a t a a a a

AseI 360 (in frame with gp41 peptide) VLIKTNVTLNIRIIERVAKY U6 promoter VLIKTNVTLNIRIIERVAKY g t a c t a a t a t c t t t g t g t t t a c a g t c a a a t t a a t t c t a a t t a t c t c t c t a a c a g c c t t g t c a t g a t t a t a g a a a c a c a a a t g t c a g t t t a a t t a a g a t t a a t a g a g a g a t t g t c g g a a c a

BsoBIAvaIPpuMIKflI 420 (in frame with gp41 peptide) RIHLYSPIMPFLGERPGRPT U6 promoter T T A G T A C C C T T T A T C C G G G A G hU6-F RIHLYSPIMPFLGERPGRPT a t c g t a t a t g c a a a t a t g a a g g a a t c a t g g g a a a t a g g c c c t c t c t g g g t c c c c t c g g g g t a g c a t a t a c g t t t a t a c t t c c t t a g t a c c c t t t a t c c g g g a g a g a c c c a g g g g a g c c c c

SfcI 480 (in frame with gp41 peptide) PLHTQFSLPSYGQRVRNVIS PLHTQFSLPSYGQRVRNVIS t t g g g a g g t g g g t c t g a a a c g a t a a t g g t g a a t a t c c c t g c c t a a c t c t a t t c a c t a t a g a a c c c t c c a c c c a g a c t t t g c t a t t a c c a c t t a t a g g g a c g g a t t g a g a t a a g t g a t a t c

540 (in frame with gp41 peptide) LVAFVIRLGVLGGVIMIFLK LVAFVIRLGVLGGVIMIFLK a a a g t a c a g c a a a a a c t a t t c t t a a a c c t a c c a a g c c t c c t a c t a t c a t t a t g a a t a a t t t t t c a t g t c g t t t t t g a t a a g a a t t t g g a t g g t t c g g a g g a t g a t a g t a a t a c t t a t t a a

600 (in frame with gp41 peptide) IYWLWNTINFWNW 15 10 LSAWKDL gp41 peptide IYWLWNTINFWNWLSAWKDL t t a t a t a c c a c a g c c a a t t t g t t a t g t t a a a c c a a t t c c a c a a a c t t g c c c a t t t a t c t a a a t a t a t g g t g t c g g t t a a a c a a t a c a a t t t g g t t a a g g t g t t t g a a c g g g t a a a t a g a t

660 5 1 ELLEQENK gp41 peptide ELLEQENKEQQNQSEEILSH a t t c c a a t a a t t c t t g t t c a t t c t t t t c t t g c t g g t t t t g c g a t t c t t c a a t t a a g g a g t t a a g g t t a t t a a g a a c a a g t a a g a a a a g a a c g a c c a a a a c g c t a a g a a g t t a a t t c c t c a

720 ILSTYNNIERDWEMWTTHNW g t a t t a a g c t t g t g t a a t t g t t a a t t t c t c t g t c c c a c t c c a t c c a g g t c g t g t g a t t c c c a t a a t t c g a a c a c a t t a a c a a t t a a a g a g a c a g g g t g a g g t a g g t c c a g c a c a c t a a g g

MslIAleIMmeIBtsαIBtsIEnd (779) StyIBsmI 779 IQELSKNSWSANWPVATTC a a a t c t g t t c c a g a g a t t t a t t a c t c c a a c t a g c a t t c c a a g g c a c a g c a g t g g t g c a a t t t a g a c a a g g t c t c t a a a t a a t g a g g t t g a t c g t a a g g t t c c g t g t c g t c a c c a c g t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.