Addgene p3E-P2A-CFP no-pA - Sequence Analyzer Skip to main content
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Sequence Analyzer: p3E-P2A-CFP no-pA


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NdeI (3442) AflII (3360) AhdI (3355) M13 Forward (3342 .. 3359) M13/pUC Forward (3328 .. 3350) BbsI (3243) AclI (3170) BsaHI (3133) rrnB T2 terminator PvuII (2980) BspQI - SapI (2917) L4440 (2898 .. 2915) NspI (2804) PciI (2800) DrdI (2698) pBR322ori-F (2645 .. 2664) BciVI (2602) PspFI (2500) BseYI (2496) ApaLI (2486) AlwNI (2391) PflMI (1888) BspEI * (32) BmgBI (73) PaeR7I - XhoI (110) BseRI (146) EGFP-N (161 .. 182) BtgZI (237) PfoI * (396) EXFP-R (422 .. 441) EGFP-C (769 .. 790) HindIII (836) TspMI - XmaI (860) SmaI (862) SpeI (872) pBluescriptSK (867 .. 883) SacII (897) BstXI (898) PsiI (941) NcoI - StyI (1017) T7 promoter T7 (1047 .. 1066) M13 Reverse (1071 .. 1087) pENTR-R (1189 .. 1208) NruI (1282) Kan-R (1267 .. 1286) EcoNI (1537) SspI (1550) AsiSI - PvuI (1625) p3E-P2A-CFP no-pA 3539 bp

Sequence View

BspEI* 60 1 5 ATNFSLLK attR2 P2A c t t g t a c a a a g t g g a a g a a t t g g g t a c c g g a t c c g g a g c c a c g a a c t t c t c t c t g t t a a a g a a c a t g t t t c a c c t t c t t a a c c c a t g g c c t a g g c c t c g g t g c t t g a a g a g a g a c a a t t t

PaeR7IXhoIBmgBI 120 10 15 QAGDVEENPGP (in frame with P2A) GTGPPLE 1 M 1a V P2A ECFP MV g c a a g c a g g a g a c g t g g a a g a a a a c c c c g g t c c t g g t a c c g g g c c c c c c c t c g a g a t g g t c g t t c g t c c t c t g c a c c t t c t t t t g g g g c c a g g a c c a t g g c c c g g g g g g g a g c t c t a c c a

BseRI 180 5 10 15 20 SKGEELFTGVVPILVELDGD ECFP G A C C A G C T C G A C C T G C C G C T EGFP-N SKGEELFTGVVPILVELDGD g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t

BtgZI 240 25 30 35 40 VNGHKFSVSGEGEGDATYGK ECFP G C EGFP-N VNGHKFSVSGEGEGDATYGK c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t

300 45 50 55 60 LTLKFICTTGKLPVPWPTLV ECFP LTLKFICTTGKLPVPWPTLV g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a

360 65 70 75 80 TTLTWGVQCFSRYPDHMKQH ECFP TTLTWGVQCFSRYPDHMKQH g a c c a c c c t g a c c t g g g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c t g g t g g g a c t g g a c c c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c g t

PfoI* 420 85 90 95 100 DFFKSAMPEGYVQERTIFFK ECFP DFFKSAMPEGYVQERTIFFK c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t

480 105 110 115 120 DDGNYKTRAEVKFEGDTLVN ECFP C T G C T G C C G T T G A T G T T C T G EXFP-R DDGNYKTRAEVKFEGDTLVN g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t

540 125 130 135 140 RIELKGIDFKEDGNILGHKL ECFP RIELKGIDFKEDGNILGHKL c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a

600 145 150 155 160 EYNYISHNVYITADKQKNGI ECFP EYNYISHNVYITADKQKNGI g g a g t a c a a c t a c a t c a g c c a c a a c g t c t a t a t c a c c g c c g a c a a g c a g a a g a a c g g c a t c c t c a t g t t g a t g t a g t c g g t g t t g c a g a t a t a g t g g c g g c t g t t c g t c t t c t t g c c g t a

660 165 170 175 180 KANFKIRHNIEDGSVQLADH ECFP KANFKIRHNIEDGSVQLADH c a a g g c c a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a g t t c c g g t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t

720 185 190 195 200 YQQNTPIGDGPVLLPDNHYL ECFP YQQNTPIGDGPVLLPDNHYL c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a

780 205 210 215 220 STQSALSKDPNEKRDHMVLL ECFP C A T G G T C C T G C T EGFP-C STQSALSKDPNEKRDHMVLL g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a

HindIII 840 225 230 235 EFVTAAGITLGMDELYK* ECFP G G A G T T C G T G EGFP-C EFVTAAGITLGMDELYK* g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g t a a a a g c t c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c a t t t t c g a

SacIIBstXISpeITspMIXmaISmaI 900 SK primer C T A G G T G A T C A A G A T C T pBluescriptSK t g a t a t c g a a t t c c t g c a g c c c g g g g g a t c c a c t a g t t c t a g a g c g g c c g c c a c c g c g g t a c t a t a g c t t a a g g a c g t c g g g c c c c c t a g g t g a t c a a g a t c t c g c c g g c g g t g g c g c c a

PsiI 960 attL3 g g a g c t c c a g c t t g c a a c t t t a t t a t a c a a a g t t g g c a t t a t a a a a a a g c a t t g c t t a t c c c t c g a g g t c g a a c g t t g a a a t a a t a t g t t t c a a c c g t a a t a t t t t t t c g t a a c g a a t a g

NcoIStyI 1020 attL3 a a t t t g t t g c a a c g a a c a g g t c a c t a t c a g t c a a a a t a a a a t c a t t a t t t g g a g c t c c a t t t a a a c a a c g t t g c t t g t c c a g t g a t a g t c a g t t t t a t t t t a g t a a t a a a c c t c g a g g t a

1080 T7 promoter M13 rev G G G A T A T C A C T C A G C A T A A T T7 C A G T A T C G A C M13 Reverse g g t a g c g t t a a c g c g g c c g c g a t a t c c c c t a t a g t g a g t c g t a t t a c a t g g t c a t a g c t g c c a t c g c a a t t g c g c c g g c g c t a t a g g g g a t a t c a c t c a g c a t a a t g t a c c a g t a t c g a c

1140 M13 rev A A A G G A C M13 Reverse t t t c c t g g c a g c t c t g g c c c g t g t c t c a a a a t c t c t g a t g t t a c a t t g c a c a a g a t a a a a a a a g g a c c g t c g a g a c c g g g c a c a g a g t t t t a g a g a c t a c a a t g t a a c g t g t t c t a t t t t

1200 KanR G T T C C C C A C A A T pENTR-R a t a t a t c a t c a t g a a c a a t a a a a c t g t c t g c t t a c a t a a a c a g t a a t a c a a g g g g t g t t a t a t a t a g t a g t a c t t g t t a t t t t g a c a g a c g a a t g t a t t t g t c a t t a t g t t c c c c a c a a t

1260 1 5 10 15 20 MSHIQRETSRPRLNSNMDAD KanR A C T C G G T A pENTR-R MSHIQRETSRPRLNSNMDAD t g a g c c a t a t t c a a c g g g a a a c g t c g a g g c c g c g a t t a a a t t c c a a c a t g g a t g c t g a t t a c t c g g t a t a a g t t g c c c t t t g c a g c t c c g g c g c t a a t t t a a g g t t g t a c c t a c g a c t a a

NruI 1320 25 30 35 40 LYGYKWARDNVGQSGATIYR KanR C C A T A T T T A C C C G A G C G C T A Kan-R LYGYKWARDNVGQSGATIYR t a t a t g g g t a t a a a t g g g c t c g c g a t a a t g t c g g g c a a t c a g g t g c g a c a a t c t a t c g c t a t a t a c c c a t a t t t a c c c g a g c g c t a t t a c a g c c c g t t a g t c c a c g c t g t t a g a t a g c g a

1380 45 50 55 60 LYGKPDAPELFLKHGKGSVA KanR LYGKPDAPELFLKHGKGSVA t g t a t g g g a a g c c c g a t g c g c c a g a g t t g t t t c t g a a a c a t g g c a a a g g t a g c g t t g c c a a c a t a c c c t t c g g g c t a c g c g g t c t c a a c a a a g a c t t t g t a c c g t t t c c a t c g c a a c g g t

1440 65 70 75 80 NDVTDEMVRLNWLTEFMPLP KanR NDVTDEMVRLNWLTEFMPLP a t g a t g t t a c a g a t g a g a t g g t c a g a c t a a a c t g g c t g a c g g a a t t t a t g c c t c t t c c g a t a c t a c a a t g t c t a c t c t a c c a g t c t g a t t t g a c c g a c t g c c t t a a a t a c g g a g a a g g c t

1500 85 90 95 100 TIKHFIRTPDDAWLLTTAIP KanR TIKHFIRTPDDAWLLTTAIP c c a t c a a g c a t t t t a t c c g t a c t c c t g a t g a t g c a t g g t t a c t c a c c a c t g c g a t c c c c g g g t a g t t c g t a a a a t a g g c a t g a g g a c t a c t a c g t a c c a a t g a g t g g t g a c g c t a g g g g c

SspIEcoNI 1560 105 110 115 120 GKTAFQVLEEYPDSGENIVD KanR GKTAFQVLEEYPDSGENIVD g a a a a a c a g c a t t c c a g g t a t t a g a a g a a t a t c c t g a t t c a g g t g a a a a t a t t g t t g a t g c t t t t t g t c g t a a g g t c c a t a a t c t t c t t a t a g g a c t a a g t c c a c t t t t a t a a c a a c t a c

1620 125 130 135 140 ALAVFLRRLHSIPVCNCPFN KanR ALAVFLRRLHSIPVCNCPFN c g c t g g c a g t g t t c c t g c g c c g g t t g c a t t c g a t t c c t g t t t g t a a t t g t c c t t t t a a c a g c g a c c g t c a c a a g g a c g c g g c c a a c g t a a g c t a a g g a c a a a c a t t a a c a g g a a a a t t g t

AsiSIPvuI 1680 145 150 155 160 SDRVFRLAQAQSRMNNGLVD KanR SDRVFRLAQAQSRMNNGLVD g c g a t c g c g t a t t t c g t c t c g c t c a g g c g c a a t c a c g a a t g a a t a a c g g t t t g g t t g a t g c g c t a g c g c a t a a a g c a g a g c g a g t c c g c g t t a g t g c t t a c t t a t t g c c a a a c c a a c t a c

1740 165 170 175 180 ASDFDDERNGWPVEQVWKEM KanR ASDFDDERNGWPVEQVWKEM c g a g t g a t t t t g a t g a c g a g c g t a a t g g c t g g c c t g t t g a a c a a g t c t g g a a a g a a a t g c g c t c a c t a a a a c t a c t g c t c g c a t t a c c g a c c g g a c a a c t t g t t c a g a c c t t t c t t t a c g

1800 185 190 195 200 HKLLPFSPDSVVTHGDFSLD KanR HKLLPFSPDSVVTHGDFSLD a t a a a c t t t t g c c a t t c t c a c c g g a t t c a g t c g t c a c t c a t g g t g a t t t c t c a c t t g a t a t a t t t g a a a a c g g t a a g a g t g g c c t a a g t c a g c a g t g a g t a c c a c t a a a g a g t g a a c t a t

1860 205 210 215 220 NLIFDEGKLIGCIDVGRVGI KanR NLIFDEGKLIGCIDVGRVGI a c c t t a t t t t t g a c g a g g g g a a a t t a a t a g g t t g t a t t g a t g t t g g a c g a g t c g g a a t c g t g g a a t a a a a a c t g c t c c c c t t t a a t t a t c c a a c a t a a c t a c a a c c t g c t c a g c c t t a g c

PflMI 1920 225 230 235 240 ADRYQDLAILWNCLGEFSPS KanR ADRYQDLAILWNCLGEFSPS c a g a c c g a t a c c a g g a t c t t g c c a t c c t a t g g a a c t g c c t c g g t g a g t t t t c t c c t t c a t g t c t g g c t a t g g t c c t a g a a c g g t a g g a t a c c t t g a c g g a g c c a c t c a a a a g a g g a a g t a

1980 245 250 255 260 LQKRLFQKYGIDNPDMNKLQ KanR LQKRLFQKYGIDNPDMNKLQ t a c a g a a a c g g c t t t t t c a a a a a t a t g g t a t t g a t a a t c c t g a t a t g a a t a a a t t g c a g t a t g t c t t t g c c g a a a a a g t t t t t a t a c c a t a a c t a t t a g g a c t a t a c t t a t t t a a c g t c a

2040 265 270 FHLMLDEFF* KanR FHLMLDEFF* t t c a t t t g a t g c t c g a t g a g t t t t t c t a a t c a g a a t t g g t t a a t t g g t t g t a a c a c t g g c a a g t a a a c t a c g a g c t a c t c a a a a a g a t t a g t c t t a a c c a a t t a a c c a a c a t t g t g a c c g

2100 a g a g c a t t a c g c t g a c t t g a c g g g a c g g c g c a a g c t c a t g a c c a a a a t c c c t t a a c g t g a t c t c g t a a t g c g a c t g a a c t g c c c t g c c g c g t t c g a g t a c t g g t t t t a g g g a a t t g c a c t

2160 ori g t t a c g c g t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g c a a t g c g c a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c

2220 ori a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c

2280 ori t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t

2340 ori g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t

AlwNI 2400 ori a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t

2460 ori g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g

BseYIPspFIApaLI 2520 ori a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t

2580 ori c c g a a c t g a g a t a c c t a c a g c g t g a g c a t t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a g g c t t g a c t c t a t g g a t g t c g c a c t c g t a a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t

BciVI 2640 ori a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g

DrdI 2700 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g

2760 ori g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c

NspIPciI 2820 c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a

2880 c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t

BspQISapI 2940 A G C G A G T C A G T G A G C G A G L4440 g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t

PvuII 3000 a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c

3060 a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t a c g c g t a c c g c t a g c c a g g a a g a g t t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a t g c g c a t g g c g a t c g g t c c t t c t c a

3120 rrnB T2 terminator t t g t a g a a a c g c a a a a a g g c c a t c c g t c a g g a t g g c c t t c t g c t t a g t t t g a t g c c t g g c a a c a t c t t t g c g t t t t t c c g g t a g g c a g t c c t a c c g g a a g a c g a a t c a a a c t a c g g a c c g

AclIBsaHI 3180 a g t t t a t g g c g g g c g t c c t g c c c g c c a c c c t c c g g g c c g t t g c t t c a c a a c g t t c a a a t c t c a a a t a c c g c c c g c a g g a c g g g c g g t g g g a g g c c c g g c a a c g a a g t g t t g c a a g t t t a g

3240 rrnB T1 terminator c g c t c c c g g c g g a t t t g t c c t a c t c a g g a g a g c g t t c a c c g a c a a a c a a c a g a t a a a a c g g c g a g g g c c g c c t a a a c a g g a t g a g t c c t c t c g c a a g t g g c t g t t t g t t g t c t a t t t t g c

BbsI 3300 rrnB T1 terminator a a a g g c c c a g t c t t c c g a c t g a g c c t t t c g t t t t a t t t g a t g c c t g g c a g t t c c c t a c t c t t t c c g g g t c a g a a g g c t g a c t c g g a a a g c a a a a t a a a c t a c g g a c c g t c a a g g g a t g a g

AhdI 3360 M13 fwd C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward t c g c g t t a a c g c t a g c a t g g a t g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t c a g c g c a a t t g c g a t c g t a c c t a c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a g

AflII 3420 t t a a g c t c g g g c c c t g c a g c t c t a g a g c t c g a a t t c t a c a g g t c a c t a a t a c c a t c t a a g a a t t c g a g c c c g g g a c g t c g a g a t c t c g a g c t t a a g a t g t c c a g t g a t t a t g g t a g a t t c

NdeI 3480 attR2 t a g t t g a t t c a t a g t g a c t g c a t a t g t t g t g t t t t a c a g t a t t a t g t a g t c t g t t t t t t a a t c a a c t a a g t a t c a c t g a c g t a t a c a a c a c a a a a t g t c a t a a t a c a t c a g a c a a a a a a t

3539 attR2 t g c a a a a t c t a a t t t a a t a t a t t g a t a t t t a t a t c a t t t t a c g t t t c t c g t t c a a c t t t a c g t t t t a g a t t a a a t t a t a t a a c t a t a a a t a t a g t a a a a t g c a a a g a g c a a g t t g a a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.