Addgene PA-RL-mCherry - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: PA-RL-mCherry


Map View

SgrDI (0) SspI (7371) Amp-R (7117 .. 7136) ScaI (7047) PvuI (6937) pBR322ori-F (5818 .. 5837) PciI (5677) L4440 (5567 .. 5584) CAP binding site lac promoter M13/pUC Reverse (5349 .. 5371) lac operator M13 Reverse (5336 .. 5352) M13 rev BstZ17I (5298) EBV-rev (5257 .. 5276) BsmI (5246) SV40pA-R (5203 .. 5222) BstBI (5008) Neo-F (4862 .. 4881) RsrII (4842) BssHII (4723) Neo-R (4252 .. 4271) SmaI (4139) TspMI - XmaI (4137) AvrII (4116) SV40pro-F (4044 .. 4063) CsiI - SexAI * (3883) pBABE 3' (3797 .. 3817) pRS-marker (46 .. 65) MfeI (163) SpeI (251) CMV enhancer NdeI (486) SnaBI (592) CMV-F (771 .. 791) T7 (865 .. 884) NheI (897) BmtI (901) AflII (910) HindIII (913) Acc65I (919) KpnI (923) BamHI (931) Kozak sequence EcoRI (1326) Bsu36I (1919) PasI (2107) PaeR7I - PspXI - XhoI (2266) mCherry-R (2450 .. 2468) SbfI (2660) mCherry-F (2718 .. 2737) PflMI (2744) BbvCI (2843) BsgI (2899) AleI (2947) SgrAI (2986) XcmI (2991) PpuMI (3012) attB2 NotI (3046) XbaI (3053) PspOMI (3059) ApaI (3063) BGH-rev (3084 .. 3101) F1ori-R (3447 .. 3466) F1ori-F (3657 .. 3678) PA-RL-mCherry 7488 bp

Sequence View

SgrDI 5ʹ 3ʹ 60 A T G T T A G A C G A G A C T pRS-marker t c g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a a g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t

120 A C G G C pRS-marker t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c

MfeI 180 c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a

240 CMV enhancer g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t

SpeI 300 CMV enhancer t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t

360 CMV enhancer t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t

420 CMV enhancer c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a

480 CMV enhancer c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a

NdeI 540 CMV enhancer g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t

SnaBI 600 CMV enhancer t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a

660 CMV enhancer CMV promoter c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a

720 CMV promoter t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t

780 CMV promoter C G C A A A T G G G CMV-F c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c

840 CMV promoter C G G T A G G C G T G CMV-F c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c t c t g g c t a a c t a g a g a a c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g a g a c c g a t t g a t c t c t t g g

NheI 900 T7 promoter T A A T A C G A C T C A C T A T A G G G T7 c a c t g c t t a c t g g c t t a t c g a a a t t a a t a c g a c t c a c t a t a g g g a g a c c c a a g c t g g c t a g t g a c g a a t g a c c g a a t a g c t t t a a t t a t g c t g a g t g a t a t c c c t c t g g g t t c g a c c g a t

BamHIBmtIAflIIHindIIIAcc65IKpnI 960 Kozak sequence MVAQH g c g t t t a a a c t t a a g c t t g g t a c c g a g c t c g g a t c c g c c g c c a c c a t g g t t g c g c a a c a c c g c a a a t t t g a a t t c g a a c c a t g g c t c g a g c c t a g g c g g c g g t g g t a c c a a c g c g t t g t g

1020 1 5 10 15 VDNKFNKEQQNAFYEIL ProtA DEAVDNKFNKEQQNAFYEIL g a t g a a g c c g t g g a c a a c a a a t t c a a c a a a g a a c a a c a a a a c g c g t t c t a t g a g a t c t t a c t a c t t c g g c a c c t g t t g t t t a a g t t g t t t c t t g t t g t t t t g c g c a a g a t a c t c t a g a a t

1080 20 25 30 35 HLPNLNEEQRNAFIQSLKDD ProtA HLPNLNEEQRNAFIQSLKDD c a t t t a c c t a a c t t a a a c g a a g a a c a a c g a a a c g c c t t c a t c c a a a g t t t a a a a g a t g a c g t a a a t g g a t t g a a t t t g c t t c t t g t t g c t t t g c g g a a g t a g g t t t c a a a t t t t c t a c t g

1140 40 45 50 55 PSQSANLLAEAKKLNDAQAP ProtA PSQSANLLAEAKKLNDAQAP c c a a g c c a a a g c g c t a a c c t t t t a g c a g a a g c t a a a a a g c t a a a t g a t g c t c a g g c g c c g g g t t c g g t t t c g c g a t t g g a a a a t c g t c t t c g a t t t t t c g a t t t a c t a c g a g t c c g c g g c

1200 K 1 5 10 15 VDNKFNKEQQNAFYEILHL ProtA ProtA KVDNKFNKEQQNAFYEILHL a a a g t a g a c a a c a a a t t c a a c a a a g a a c a a c a a a a c g c g t t c t a t g a g a t c t t a c a t t t a t t t c a t c t g t t g t t t a a g t t g t t t c t t g t t g t t t t g c g c a a g a t a c t c t a g a a t g t a a a t

1260 20 25 30 35 PNLNEEQRNAFIQSLKDDPS ProtA PNLNEEQRNAFIQSLKDDPS c c t a a c t t a a a c g a a g a a c a a c g a a a c g c c t t c a t c c a a a g t t t a a a a g a t g a c c c a a g c g g a t t g a a t t t g c t t c t t g t t g c t t t g c g g a a g t a g g t t t c a a a t t t t c t a c t g g g t t c g

1320 40 45 50 55 QSANLLAEAKKLNGAQAPK V ProtA QSANLLAEAKKLNGAQAPKV c a a a g c g c t a a c c t t t t a g c a g a a g c t a a a a a g c t a a a t g g t g c t c a g g c g c c g a a a g t a g t t t c g c g a t t g g a a a a t c g t c t t c g a t t t t t c g a t t t a c c a c g a g t c c g c g g c t t t c a t

EcoRI 1380 DANS 1 5 10 15 MASKVYDPEQRKRMIT hRluc DANSMASKVYDPEQRKRMIT g a c g c g a a t t c g a t g g c t t c c a a g g t g t a c g a c c c c g a g c a a c g c a a a c g c a t g a t c a c t c t g c g c t t a a g c t a c c g a a g g t t c c a c a t g c t g g g g c t c g t t g c g t t t g c g t a c t a g t g a

1440 20 25 30 35 GPQWWARCKQMNVLDSFINY hRluc GPQWWARCKQMNVLDSFINY g g g c c t c a g t g g t g g g c t c g c t g c a a g c a a a t g a a c g t g c t g g a c t c c t t c a t c a a c t a c c c c g g a g t c a c c a c c c g a g c g a c g t t c g t t t a c t t g c a c g a c c t g a g g a a g t a g t t g a t g

1500 40 45 50 55 YDSEKHAENAVIFLHGNAAS hRluc YDSEKHAENAVIFLHGNAAS t a t g a t t c c g a g a a g c a c g c c g a g a a c g c c g t g a t t t t t c t g c a t g g t a a c g c t g c c t c c a t a c t a a g g c t c t t c g t g c g g c t c t t g c g g c a c t a a a a a g a c g t a c c a t t g c g a c g g a g g

1560 60 65 70 75 SYLWRHVVPHIEPVARCIIP hRluc SYLWRHVVPHIEPVARCIIP a g c t a c c t g t g g a g g c a c g t c g t g c c t c a c a t c g a g c c c g t g g c t a g a t g c a t c a t c c c t t c g a t g g a c a c c t c c g t g c a g c a c g g a g t g t a g c t c g g g c a c c g a t c t a c g t a g t a g g g a

1620 80 85 90 95 DLIGMGKSGKSGNGSYRLLD hRluc DLIGMGKSGKSGNGSYRLLD g a t c t g a t c g g a a t g g g t a a g t c c g g c a a g a g c g g g a a t g g c t c a t a t c g c c t c c t g g a t c t a g a c t a g c c t t a c c c a t t c a g g c c g t t c t c g c c c t t a c c g a g t a t a g c g g a g g a c c t a

1680 100 105 110 115 HYKYLTAWFELLNLPKKIIF hRluc HYKYLTAWFELLNLPKKIIF c a c t a c a a g t a c c t c a c c g c t t g g t t c g a g c t g c t g a a c c t t c c a a a g a a a a t c a t c t t t g t g a t g t t c a t g g a g t g g c g a a c c a a g c t c g a c g a c t t g g a a g g t t t c t t t t a g t a g a a a

1740 120 125 130 135 VGHDWGACLAFHYSYEHQDK hRluc VGHDWGACLAFHYSYEHQDK g t g g g c c a c g a c t g g g g g g c t t g t c t g g c c t t t c a c t a c t c c t a c g a g c a c c a a g a c a a g c a c c c g g t g c t g a c c c c c c g a a c a g a c c g g a a a g t g a t g a g g a t g c t c g t g g t t c t g t t c

1800 140 145 150 155 IKAIVHAESVVDVIESWDEW hRluc IKAIVHAESVVDVIESWDEW a t c a a g g c c a t c g t c c a t g c t g a g a g t g t c g t g g a c g t g a t c g a g t c c t g g g a c g a g t g g t a g t t c c g g t a g c a g g t a c g a c t c t c a c a g c a c c t g c a c t a g c t c a g g a c c c t g c t c a c c

1860 160 165 170 175 PDIEEDIALIKSEEGEKMVL hRluc PDIEEDIALIKSEEGEKMVL c c t g a c a t c g a g g a g g a t a t c g c c c t g a t c a a g a g c g a a g a g g g c g a g a a a a t g g t g c t t g g a c t g t a g c t c c t c c t a t a g c g g g a c t a g t t c t c g c t t c t c c c g c t c t t t t a c c a c g a a

Bsu36I 1920 180 185 190 195 ENNFFVETMLPSKIMRKLEP hRluc ENNFFVETMLPSKIMRKLEP g a g a a t a a c t t c t t c g t c g a g a c c a t g c t c c c a a g c a a g a t c a t g c g g a a a c t g g a g c c t c t c t t a t t g a a g a a g c a g c t c t g g t a c g a g g g t t c g t t c t a g t a c g c c t t t g a c c t c g g a

1980 200 205 210 215 EEFAAYLEPFKEKGEVRRPT hRluc EEFAAYLEPFKEKGEVRRPT g a g g a g t t c g c t g c c t a c c t g g a g c c a t t c a a g g a g a a g g g c g a g g t t a g a c g g c c t a c c c t c c t c a a g c g a c g g a t g g a c c t c g g t a a g t t c c t c t t c c c g c t c c a a t c t g c c g g a t g g

2040 220 225 230 235 LSWPREIPLVKGGKPDVVQI hRluc LSWPREIPLVKGGKPDVVQI c t c t c c t g g c c t c g c g a g a t c c c t c t c g t t a a g g g a g g c a a g c c c g a c g t c g t c c a g a t t g a g a g g a c c g g a g c g c t c t a g g g a g a g c a a t t c c c t c c g t t c g g g c t g c a g c a g g t c t a a

2100 240 245 250 255 VRNYNAYLRASDDLPKMFIE hRluc VRNYNAYLRASDDLPKMFIE g t c c g c a a c t a c a a c g c c t a c c t t c g g g c c a g c g a c g a t c t g c c t a a g a t g t t c a t c g a g c a g g c g t t g a t g t t g c g g a t g g a a g c c c g g t c g c t g c t a g a c g g a t t c t a c a a g t a g c t c

PasI 2160 260 265 270 275 SDPGFFSNAIVEGAKKFPNT hRluc SDPGFFSNAIVEGAKKFPNT t c c g a c c c t g g g t t c t t t t c c a a c g c t a t t g t c g a g g g a g c t a a g a a g t t c c c t a a c a c c a g g c t g g g a c c c a a g a a a a g g t t g c g a t a a c a g c t c c c t c g a t t c t t c a a g g g a t t g t g g

2220 280 285 290 295 EFVKVKGLHFSQEDAPDEMG hRluc EFVKVKGLHFSQEDAPDEMG g a g t t c g t g a a g g t g a a g g g c c t c c a c t t c a g c c a g g a g g a c g c t c c a g a t g a a a t g g g t c t c a a g c a c t t c c a c t t c c c g g a g g t g a a g t c g g t c c t c c t g c g a g g t c t a c t t t a c c c a

PaeR7IPspXIXhoI 2280 300 305 310 KYIKSFVERVLKNEQ (in frame with hRluc) LEDIT hRluc attB1 KYIKSFVERVLKNEQLEDIT a a g t a c a t c a a g a g c t t c g t g g a g c g c g t g c t g a a g a a c g a g c a g c t c g a g g a t a t c a c a t t c a t g t a g t t c t c g a a g c a c c t c g c g c a c g a c t t c t t g c t c g t c g a g c t c c t a t a g t g t

2340 (in frame with hRluc) SLYKKAGF 1 5 10 MVSKGEEDNMAI mCherry attB1 SLYKKAGFMVSKGEEDNMAI a g t t t g t a c a a a a a a g c a g g c t t c a t g g t g a g c a a g g g c g a g g a g g a t a a c a t g g c c a t c t c a a a c a t g t t t t t t c g t c c g a a g t a c c a c t c g t t c c c g c t c c t c c t a t t g t a c c g g t a g

2400 15 20 25 30 IKEFMRFKVHMEGSVNGHEF mCherry IKEFMRFKVHMEGSVNGHEF a t c a a g g a g t t c a t g c g c t t c a a g g t g c a c a t g g a g g g c t c c g t g a a c g g c c a c g a g t t c t a g t t c c t c a a g t a c g c g a a g t t c c a c g t g t a c c t c c c g a g g c a c t t g c c g g t g c t c a a g

2460 35 40 45 50 EIEGEGEGRPYEGTQTAKLK mCherry G G T T C G A C T T C mCherry-R EIEGEGEGRPYEGTQTAKLK g a g a t c g a g g g c g a g g g c g a g g g c c g c c c c t a c g a g g g c a c c c a g a c c g c c a a g c t g a a g c t c t a g c t c c c g c t c c c g c t c c c g g c g g g g a t g c t c c c g t g g g t c t g g c g g t t c g a c t t c

2520 55 60 65 70 VTKGGPLPFAWDILSPQFMY mCherry C A C T G G T T mCherry-R VTKGGPLPFAWDILSPQFMY g t g a c c a a g g g t g g c c c c c t g c c c t t c g c c t g g g a c a t c c t g t c c c c t c a g t t c a t g t a c c a c t g g t t c c c a c c g g g g g a c g g g a a g c g g a c c c t g t a g g a c a g g g g a g t c a a g t a c a t g

2580 75 80 85 90 GSKAYVKHPADIPDYLKLSF mCherry GSKAYVKHPADIPDYLKLSF g g c t c c a a g g c c t a c g t g a a g c a c c c c g c c g a c a t c c c c g a c t a c t t g a a g c t g t c c t t c c c g a g g t t c c g g a t g c a c t t c g t g g g g c g g c t g t a g g g g c t g a t g a a c t t c g a c a g g a a g

2640 95 100 105 110 PEGFKWERVMNFEDGGVVTV mCherry PEGFKWERVMNFEDGGVVTV c c c g a g g g c t t c a a g t g g g a g c g c g t g a t g a a c t t c g a g g a c g g c g g c g t g g t g a c c g t g g g g c t c c c g a a g t t c a c c c t c g c g c a c t a c t t g a a g c t c c t g c c g c c g c a c c a c t g g c a c

SbfI 2700 115 120 125 130 TQDSSLQDGEFIYKVKLRGT mCherry TQDSSLQDGEFIYKVKLRGT a c c c a g g a c t c c t c c c t g c a g g a c g g c g a g t t c a t c t a c a a g g t g a a g c t g c g c g g c a c c t g g g t c c t g a g g a g g g a c g t c c t g c c g c t c a a g t a g a t g t t c c a c t t c g a c g c g c c g t g g

PflMI 2760 135 140 145 150 NFPSDGPVMQKKTMGWEASS mCherry C C C C G T A A T G C A G A A G A A G A mCherry-F NFPSDGPVMQKKTMGWEASS a a c t t c c c c t c c g a c g g c c c c g t a a t g c a g a a g a a g a c c a t g g g c t g g g a g g c c t c c t c c t t g a a g g g g a g g c t g c c g g g g c a t t a c g t c t t c t t c t g g t a c c c g a c c c t c c g g a g g a g g

2820 155 160 165 170 ERMYPEDGALKGEIKQRLKL mCherry ERMYPEDGALKGEIKQRLKL g a g c g g a t g t a c c c c g a g g a c g g c g c c c t g a a g g g c g a g a t c a a g c a g a g g c t g a a g c t g c t c g c c t a c a t g g g g c t c c t g c c g c g g g a c t t c c c g c t c t a g t t c g t c t c c g a c t t c g a c

BbvCI 2880 175 180 185 190 KDGGHYDAEVKTTYKAKKPV mCherry KDGGHYDAEVKTTYKAKKPV a a g g a c g g c g g c c a c t a c g a c g c t g a g g t c a a g a c c a c c t a c a a g g c c a a g a a g c c c g t g t t c c t g c c g c c g g t g a t g c t g c g a c t c c a g t t c t g g t g g a t g t t c c g g t t c t t c g g g c a c

BsgI 2940 195 200 205 210 QLPGAYNVNIKLDITSHNED mCherry QLPGAYNVNIKLDITSHNED c a g c t g c c c g g c g c c t a c a a c g t c a a c a t c a a g t t g g a c a t c a c c t c c c a c a a c g a g g a c g t c g a c g g g c c g c g g a t g t t g c a g t t g t a g t t c a a c c t g t a g t g g a g g g t g t t g c t c c t g

XcmISgrAIAleI 3000 215 220 225 230 YTIVEQYERAEGRHSTGGMD mCherry YTIVEQYERAEGRHSTGGMD t a c a c c a t c g t g g a a c a g t a c g a a c g c g c c g a g g g c c g c c a c t c c a c c g g c g g c a t g g a c a t g t g g t a g c a c c t t g t c a t g c t t g c g c g g c t c c c g g c g g t g a g g t g g c c g c c g t a c c t g

PspOMIXbaINotIPpuMI 3060 235 ELYK (in frame with mCherry) DPAFLYKVVISRPL* mCherry attB2 ELYKDPAFLYKVVISRPL* g a g c t g t a c a a g g a c c c a g c t t t c t t g t a c a a a g t g g t g a t a t c g c g g c c g c t c t a g a g g c t c g a c a t g t t c c t g g g t c g a a a g a a c a t g t t t c a c c a c t a t a g c g c c g g c g a g a t c t c c

ApaI 3120 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a

3180 bGH poly(A) signal t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t

3240 bGH poly(A) signal a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g g t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c c

3300 bGH poly(A) signal g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a t g c t g g g g a t g c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t a c g a c c c c t a c g

3360 bGH poly(A) signal f1 ori g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t g g g g c t c t a g g g g g t a t c c c c a c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g a c c c c g a g a t c c c c c a t a g g g g t

3420 f1 ori c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g

3480 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g

3540 f1 ori g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c

3600 f1 ori t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g

3660 f1 ori G T G G F1ori-F a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c

3720 f1 ori A C T C T T G T T C C A A A C T G G F1ori-F a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t

3780 f1 ori a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t

3840 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t

SexAI*CsiI 3900 SV40 promoter g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g

3960 SV40 promoter c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t

4020 SV40 promoter SV40 ori g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c

4080 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c t g c c t c t g a g g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g a c g g a g a c t c

TspMIXmaISmaIAvrII 4140 SV40 promoter SV40 ori c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t c c c g g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a g g g c

4200 1 M NeoR/KanR M g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a a g a g a c a g g a t g a g g a t c g t t t c g c a t g c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t t c t c t g t c c t a c t c c t a g c a a a g c g t a c

4260 5 10 15 20 IEQDGLHAGSPAAWVERLFG NeoR/KanR G A T A A G C C G Neo-R IEQDGLHAGSPAAWVERLFG a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a a g c c g

4320 25 30 35 40 YDWAQQTIGCSDAAVFRLSA NeoR/KanR A T A C T G A C C C G Neo-R YDWAQQTIGCSDAAVFRLSA t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t c a g c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a g t c g c

4380 45 50 55 60 QGRPVLFVKTDLSGALNELQ NeoR/KanR QGRPVLFVKTDLSGALNELQ c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c t g c a g g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g a c g t c

4440 65 70 75 80 DEAARLSWLATTGVPCAAVL NeoR/KanR DEAARLSWLATTGVPCAAVL g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g t g c t c c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c a c g a g

4500 85 90 95 100 DVVTEAGRDWLLLGEVPGQD NeoR/KanR DVVTEAGRDWLLLGEVPGQD g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g t c c t a

4560 105 110 115 120 LLSSHLAPAEKVSIMADAMR NeoR/KanR LLSSHLAPAEKVSIMADAMR *RVKSRGLFYGDHSICHP c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t g c g g g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t a c g c c

4620 125 130 135 140 RLHTLDPATCPFDHQAKHRI NeoR/KanR RLHTLDPATCPFDHQAKHRI PQMRKIRSGAWEVVLRFMAD c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c g c a t c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g c g t a g

4680 145 150 155 160 ERARTRMEAGLVDQDDLDEE NeoR/KanR ERARTRMEAGLVDQDDLDEE LSCTSPHFGTKDILIIQVFL g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g a a g a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c t t c t c

BssHII 4740 165 170 175 180 HQGLAPAELFARLKARMPDG NeoR/KanR HQGLAPAELFARLKARMPDG MLPERWGFQEGPELRAHGVA c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g c g c a t g c c c g a c g g c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c g c g t a c g g g c t g c c g

4800 185 190 195 200 EDLVVTHGDACLPNIMVENG NeoR/KanR EDLVVTHGDACLPNIMVENG LIEDHGMAIGAQRIDHHFIA g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a a t g g c c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t t a c c g

RsrII 4860 205 210 215 220 RFSGFIDCGRLGVADRYQDI NeoR/KanR RFSGFIDCGRLGVADRYQDI AKRSEDVTAPQTHRVAILVY c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c t g t a t

4920 225 230 235 240 ALATRDIAEELGGEWADRFL NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F ALATRDIAEELGGEWADRFL RQSGTINSFLKAAFPSVAEE g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t c c t c c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a a g g a g

4980 245 250 255 260 VLYGIAAPDSQRIAFYRLLD NeoR/KanR VLYGIAAPDSQRIAFYRLLD HKVTDGSGIRLADGEIAKKV g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c t t g a c c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g a a c t g

BstBI 5040 265 EFF* NeoR/KanR EFF* LEESRSEPTRFSRGLSAWGA g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g c c c a a c c t g c c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c g g g t t g g a c g

5100 M c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a

5160 t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g

5220 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A SV40pA-R a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a

BsmI 5280 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev A G SV40pA-R t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c

BstZ17I 5340 SV40 poly(A) signal M13 rev C A G T A M13 Reverse t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t a a t c a t g g t c a t a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c a t t a g t a c c a g t a

5400 -10 M13 rev lac operator lac promoter T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t

5460 -35 lac promoter CAP binding site g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g

5520 g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g

5580 G A G C G A G T G A C T G A L4440 a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a

5640 G C G A L4440 c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g

PciI 5700 g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t

5760 ori a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c

5820 ori T T T pBR322ori-F a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t

5880 ori C T A T G G T C C G C A A A G G G pBR322ori-F g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g

5940 ori t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g

6000 ori g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g

6060 ori c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c

6120 ori t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a

6180 ori a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t

6240 ori c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a

6300 ori c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t t t t t t t g t t t g c a a g c a g c a g a t t a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a a a a a a a c a a a c g t t c g t c g t c t a a t

6360 ori c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g

6420 a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t

6480 c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t

6540 285 280 275 *WHKILSAGIEAIQRN AmpR *WHKILSAGIEAIQRN c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a

6600 270 265 260 255 REDMTAQSGTTYIVVIRSPK AmpR REDMTAQSGTTYIVVIRSPK t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a

6660 250 245 240 235 GDPGLAAIIGRSGREGAGSK AmpR GDPGLAAIIGRSGREGAGSK t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a

6720 230 225 220 215 DAIFWGAPLASRLLPGAVKD AmpR DAIFWGAPLASRLLPGAVKD t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a

6780 210 205 200 195 AEMWDILQQRSALTLLEGTL AmpR AEMWDILQQRSALTLLEGTL c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t

6840 190 185 180 175 LKRLTTAMAVPMTTDREDNP AmpR LKRLTTAMAVPMTTDREDNP a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c

6900 170 165 160 155 IAENLEPEWRDLRTVHDGMN AmpR IAENLEPEWRDLRTVHDGMN g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a

PvuI 6960 150 145 140 135 HLFATLEKPGGITTLLLNAA AmpR HLFATLEKPGGITTLLLNAA t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c

7020 130 125 120 115 TNDSMTIAASCLERVTMGDT AmpR TNDSMTIAASCLERVTMGDT c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c

ScaI 7080 110 105 100 95 LHKETVPSYEVLDNQSYHIR AmpR LHKETVPSYEVLDNQSYHIR t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g

7140 90 85 80 75 RGLQEQGADIRSLVAGCLLV AmpR A T A A T A C C G C G C C A C A T A G C Amp-R RGLQEQGADIRSLVAGCLLV g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t

7200 70 65 60 55 KFTSMMPFREEPRFSELIKG AmpR KFTSMMPFREEPRFSELIKG c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g

7260 50 45 40 35 SNLDLEIYGVRAGLQDEADK AmpR SNLDLEIYGVRAGLQDEADK c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a

7320 30 25 VKVLTEPH 20 15 signal sequence AFVPLCFAAFFP AmpR VKVLTEPHAFVPLCFAAFFP t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c

SspI 7380 10 5 1 signal sequence ILAVRFHQISM AmpR AmpR promoter ILAVRFHQISM g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t

7440 AmpR promoter g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t

3ʹ 5ʹ 7488 AmpR promoter a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.