Addgene pC-FLAG-ZCD2 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pC-FLAG-ZCD2 Sequencing Result

Map View

800 600 400 200 End (822) M13/pUC Forward (797 .. 819) M13 Forward (788 .. 805) T7 (758 .. 777) PsiI (777) SfcI (760) AatII (746) ZraI (744) PspFI (694) BseYI (690) SmlI - XhoI - PaeR7I (675) BtgZI (611) AclI (583) AseI (479) TsoI (410) AlwNI (337) BmrI (325) BsgI (322) XcmI (270) MscI * (262) BseRI (259) BanII Bsp1286I (238) DrdI (227) MluI - AflIII (226) SmaI (209) XmaI - TspMI (207) ApoI - EcoRI (202) KpnI (200) BanI - Acc65I (196) MflI * - BstYI - BamHI (190) DraI (167) NcoI (98) SacII (94) AvrII (85) TaqII (76) BspEI (73) BbsI (56) Start (0) 6xHis attB1 attB2 FLAG T7 promoter M13 fwd pC-FLAG-ZCD2 822 bp

Sequence View

BbsIStart (0) 5ʹ 3ʹ 60 a c c g t c a g a t c g c c t g g a g a c g c c a t c c a c g c t g t t t t g a c c t c c a t a g a a g a c a c c g g g t g g c a g t c t a g c g g a c c t c t g c g g t a g g t g c g a c a a a a c t g g a g g t a t c t t c t g t g g c c c

SacIINcoIAvrIIBspEITaqII 120 1 HHH 6xHis MAYYHHH a c c g a t c c a g c c t c c g g a c t c t a g c c t a g g c c g c g g a c c a t g g c g t a c t a c c a t c a c c a t t g g c t a g g t c g g a g g c c t g a g a t c g g a t c c g g c g c c t g g t a c c g c a t g a t g g t a g t g g t a

DraI 180 5 HHH (in frame with 6xHis) SRSTSLYKKAGSLKEPI 6xHis attB1 HHHSRSTSLYKKAGSLKEPI c a c c a t c a c t c t a g a t c a a c a a g t t t g t a c a a a a a a g c a g g c t c t t t a a a g g a a c c a a t t g t g g t a g t g a g a t c t a g t t g t t c a a a c a t g t t t t t t c g t c c g a g a a a t t t c c t t g g t t a a

Bsp1286IBanIIAflIIIMluIDrdIBamHIBstYIMflI*Acc65IBanIKpnIEcoRIApoITspMIXmaISmaI 240 (in frame with 6xHis) QSTGSGTEFPGYRRPTRPGS QSTGSGTEFPGYRRPTRPGS c a g t c g a c t g g a t c c g g t a c c g a a t t c c c g g g a t a t c g t c g a c c c a c g c g t c c g g g c t c g g t c a g c t g a c c t a g g c c a t g g c t t a a g g g c c c t a t a g c a g c t g g g t g c g c a g g c c c g a g c

XcmIBseRIMscI* 300 (in frame with 6xHis) GEEWTPLARMVLESVARIVK GEEWTPLARMVLESVARIVK g g a g a g g a g t g g a c g c c g c t g g c c a g g a t g g t g c t g g a g a g c g t g g c c c g t a t c g t g a a g c c t c t c c t c a c c t g c g g c g a c c g g t c c t a c c a c g a c c t c t c g c a c c g g g c a t a g c a c t t c

AlwNIBsgIBmrI 360 (in frame with 6xHis) VQLPAYLKRLPVPESITGFA VQLPAYLKRLPVPESITGFA g t g c a g c t c c c t g c a t a t c t g a a g c g g c t c c c a g t c c c t g a a a g c a t t a c c g g g t t c g c t c a c g t c g a g g g a c g t a t a g a c t t c g c c g a g g g t c a g g g a c t t t c g t a a t g g c c c a a g c g a

TsoI 420 (in frame with 6xHis) RLTVSEWLRLLPFLGVLALL RLTVSEWLRLLPFLGVLALL a g g c t c a c a g t t t c a g a a t g g c t t c g g t t a t t g c c t t t c c t t g g t g t a c t c g c a c t t c t t t c c g a g t g t c a a a g t c t t a c c g a a g c c a a t a a c g g a a a g g a a c c a c a t g a g c g t g a a g a a

AseI 480 (in frame with 6xHis) GYLAVRPFLPKKKQQKDSLI GYLAVRPFLPKKKQQKDSLI g g c t a c c t t g c a g t t c g t c c a t t c c t c c c g a a g a a g a a a c a a c a g a a g g a t a g c t t g a t t c c g a t g g a a c g t c a a g c a g g t a a g g a g g g c t t c t t c t t t g t t g t c t t c c t a t c g a a c t a a

540 (in frame with 6xHis) NLKIQKENPKVVNEINIEDL NLKIQKENPKVVNEINIEDL a a t c t t a a a a t a c a a a a g g a a a a t c c g a a a g t a g t g a a t g a a a t a a a c a t t g a a g a t t t g t t a g a a t t t t a t g t t t t c c t t t t a g g c t t t c a t c a c t t a c t t t a t t t g t a a c t t c t a a a c

AclI 600 (in frame with 6xHis) CLTKAAYCRCWRSKTFPACD CLTKAAYCRCWRSKTFPACD t g t c t t a c t a a a g c a g c t t a t t g t a g g t g t t g g c g t t c t a a a a c g t t t c c t g c c t g c g a t a c a g a a t g a t t t c g t c g a a t a a c a t c c a c a a c c g c a a g a t t t t g c a a a g g a c g g a c g c t a

BtgZI 660 (in frame with 6xHis) GSHNKHNELTGDNVGPLILK GSHNKHNELTGDNVGPLILK g g t t c a c a t a a t a a a c a c a a t g a a t t g a c a g g a g a t a a t g t g g g t c c a c t a a t a c t g a a g c c a a g t g t a t t a t t t g t g t t a c t t a a c t g t c c t c t a t t a c a c c c a g g t g a t t a t g a c t t c

BseYIPspFIPaeR7IXhoISmlI 720 (in frame with 6xHis) KKEVASRYLDPAFLYKVV 1 DY FLAG attB2 KKEVASRYLDPAFLYKVVDY a a g a a a g a a g t a g c c t c g a g a t a t c t a g a c c c a g c t t t c t t g t a c a a a g t g g t t g a c t a c t t c t t t c t t c a t c g g a g c t c t a t a g a t c t g g g t c g a a a g a a c a t g t t t c a c c a a c t g a t g

PsiISfcIZraIAatII 780 5 KDDDDK * FLAG T7 promoter G G G A T A T C A C T C A G C A T A A T T7 KDDDDK* a a a g a c g a t g a c g a c a a g t g a g a c g t c a t a g c t c t c t c c c t a t a g t g a g t c g t a t t a t a a t t t c t g c t a c t g c t g t t c a c t c t g c a g t a t c g a g a g a g g g a t a t c a c t c a g c a t a a t a t t

End (822) 3ʹ 5ʹ 822 M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward g c t a g g c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a c g a t c c g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.