Addgene GST-PS-Grb2 (XSB363) Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: GST-PS-Grb2 (XSB363) Sequencing Result

Map View

800 600 400 200 End (832) StyI - BtgI - NcoI (814) BamHI (810) PasI (806) ApaI - BanII - BaeGI - Bsp1286I - Bme1580I (805) PspOMI (801) pGEX 5' (734 .. 756) ScaI (696) TatI (694) BclI * (557) SwaI (550) BstBI (520) XmnI (516) BsgI (409) NspI (365) PciI - AflIII (361) MscI (330) EaeI (328) MslI (217) EarI - SapI - BspQI (202) EcoNI (133) M13/pUC Reverse (89 .. 111) AseI (55) HincII (49) SspI (31) Start (0) tac promoter lac operator GST HRV 3C site GST-PS-Grb2 (XSB363) 832 bp

Sequence View

AseIHincIISspIStart (0) 5ʹ 3ʹ 60 -35 tac promoter t t g c g c c g a c a t c a t a a c g g t t c t g g c a a a t a t t c t g a a a t g a g c t g t t g a c a a t t a a t c a a c g c g g c t g t a g t a t t g c c a a g a c c g t t t a t a a g a c t t t a c t c g a c a a c t g t t a a t t a g

120 -10 tac promoter lac operator A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse a t c g g c t c g t a t a a t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g t a t t a g c c g a g c a t a t t a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c a t a

EcoNI 180 1 5 10 15 MSPILGYWKIKGLVQPTRL GST MSPILGYWKIKGLVQPTRL t c a t g t c c c c t a t a c t a g g t t a t t g g a a a a t t a a g g g c c t t g t g c a a c c c a c t c g a c t t c a g t a c a g g g g a t a t g a t c c a a t a a c c t t t t a a t t c c c g g a a c a c g t t g g g t g a g c t g a a g

MslIBspQISapIEarI 240 20 25 30 35 LLEYLEEKYEEHLYERDEGD GST LLEYLEEKYEEHLYERDEGD t t t t g g a a t a t c t t g a a g a a a a a t a t g a a g a g c a t t t g t a t g a g c g c g a t g a a g g t g a t a a a a a c c t t a t a g a a c t t c t t t t t a t a c t t c t c g t a a a c a t a c t c g c g c t a c t t c c a c t a t

300 40 45 50 55 KWRNKKFELGLEFPNLPYYI GST KWRNKKFELGLEFPNLPYYI a a t g g c g a a a c a a a a a g t t t g a a t t g g g t t t g g a g t t t c c c a a t c t t c c t t a t t a t a t t g t t a c c g c t t t g t t t t t c a a a c t t a a c c c a a a c c t c a a a g g g t t a g a a g g a a t a a t a t a a c

EaeIMscI 360 60 65 70 75 DGDVKLTQSMAIIRYIADKH GST DGDVKLTQSMAIIRYIADKH a t g g t g a t g t t a a a t t a a c a c a g t c t a t g g c c a t c a t a c g t t a t a t a g c t g a c a a g c a c a t a c c a c t a c a a t t t a a t t g t g t c a g a t a c c g g t a g t a t g c a a t a t a t c g a c t g t t c g t g t

BsgIAflIIIPciINspI 420 80 85 90 95 NMLGGCPKERAEISMLEGAV GST NMLGGCPKERAEISMLEGAV a c a t g t t g g g t g g t t g t c c a a a a g a g c g t g c a g a g a t t t c a a t g c t t g a a g g a g c g g t t t t g t a c a a c c c a c c a a c a g g t t t t c t c g c a c g t c t c t a a a g t t a c g a a c t t c c t c g c c a a a

480 100 105 110 115 LDIRYGVSRIAYSKDFETLK GST LDIRYGVSRIAYSKDFETLK t g g a t a t t a g a t a c g g t g t t t c g a g a a t t g c a t a t a g t a a a g a c t t t g a a a c t c t c a a a g a c c t a t a a t c t a t g c c a c a a a g c t c t t a a c g t a t a t c a t t t c t g a a a c t t t g a g a g t t t c

XmnIBstBI 540 120 125 130 135 VDFLSKLPEMLKMFEDRLCH GST VDFLSKLPEMLKMFEDRLCH t t g a t t t t c t t a g c a a g c t a c c t g a a a t g c t g a a a a t g t t c g a a g a t c g t t t a t g t c a t a a a c t a a a a g a a t c g t t c g a t g g a c t t t a c g a c t t t t a c a a g c t t c t a g c a a a t a c a g t a t

BclI*SwaI 600 140 145 150 155 KTYLNGDHVTHPDFMLYDAL GST KTYLNGDHVTHPDFMLYDAL a a a c a t a t t t a a a t g g t g a t c a t g t a a c c c a t c c t g a c t t c a t g t t g t a t g a c g c t c t t g t t t g t a t a a a t t t a c c a c t a g t a c a t t g g g t a g g a c t g a a g t a c a a c a t a c t g c g a g a a c

660 160 165 170 175 DVVLYMDPMCLDAFPKLVCF GST DVVLYMDPMCLDAFPKLVCF a t g t t g t t t t a t a c a t g g a c c c a a t g t g c c t g g a t g c g t t c c c a a a a t t a g t t t g t t t t a t a c a a c a a a a t a t g t a c c t g g g t t a c a c g g a c c t a c g c a a g g g t t t t a a t c a a a c a a a a t

TatIScaI 720 180 185 190 195 KKRIEAIPQIDKYLKSSKYI GST KKRIEAIPQIDKYLKSSKYI a a a a a c g t a t t g a a g c t a t c c c a c a a a t t g a t a a g t a c t t g a a a t c c a g c a a g t a t a t a g t t t t t g c a t a a c t t c g a t a g g g t g t t t a a c t a t t c a t g a a c t t t a g g t c g t t c a t a t a t c

780 200 205 210 215 AWPLQGWQATFGGGDHPPK S GST G G G C T G G C A A G C C A C G T T T G G T G pGEX 5' AWPLQGWQATFGGGDHPPKS c a t g g c c t t t g c a g g g c t g g c a a g c c a c g t t t g g t g g t g g c g a c c a t c c t c c a a a a t c g g g t a c c g g a a a c g t c c c g a c c g t t c g g t g c a a a c c a c c a c c g c t g g t a g g a g g t t t t a g c c

End (832) PspOMIBme1580IBsp1286IBaeGIBanIIApaIPasIBamHINcoIStyIBtgI 3ʹ 5ʹ 832 D 1 5 LEVLFQGP (in frame with HRV 3C site) LGSMEAIA HRV 3C site DLEVLFQGPLGSMEAIA a t c t g g a a g t t c t g t t c c a g g g g c c c c t g g g a t c c a t g g a a g c c a t c g c c a a t a g a c c t t c a a g a c a a g g t c c c c g g g g a c c c t a g g t a c c t t c g g t a g c g g t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.