Addgene pNXRVc-HACre Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pNXRVc-HACre Sequencing Result


Map View

1000 750 500 250 End (1070) HaeII - Bpu10I (1060) ClaI - BspDI (1043) Esp3I - BsmBI (920) BtgZI (917) PaqCI (846) EcoRV (788) PsiI (739) PvuI - AsiSI (695) CsiI - SexAI * (664) BstBI (646) AclI (607) EciI (605) BsrDI (572) RsrII (538) TaqII (526) BssHII (474) NruI (451) XmnI (445) AlwNI (438) PciI - AflIII (322) AgeI (278) ApoI (263) MscI (240) EaeI (238) EarI (210) TatI (198) XbaI (192) BmgBI (140) BmtI (94) NheI (90) PacI (85) SmaI (77) XmaI - TspMI (75) HincII (66) AccI (65) SalI (64) PstI (62) SfcI (58) BsiWI (52) HindIII (46) SacI - BanII - BsiHKAI - Bsp1286I (44) Eco53kI (42) XhoI - PaeR7I (37) BpuEI (28) Start (0) HA HA HA SV40 NLS pNXRVc-HACre 1070 bp

Sequence View

SfcIBsiWIPaeR7IXhoIEco53kIBsp1286IBsiHKAIBanIISacIHindIIIBpuEIStart (0) 60 SWFSEPSDPLGSRAQASYA a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g g a t c t c g a g c t c a a g c t t c g t a c g c t g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c c t a g a g c t c g a g t t c g a a g c a t g c g a c

BmtINheIPacITspMIXmaISmaIPstISalIAccIHincII 120 1 5 YPYDVPD HA AGRRIPGLINASMYPYDVPD c a g g t c g a c g g a t c c c c g g g t t a a t t a a c g c t a g c a t g t a c c c a t a c g a t g t t c c t g a c t g t c c a g c t g c c t a g g g g c c c a a t t a a t t g c g a t c g t a c a t g g g t a t g c t a c a a g g a c t g a

BmgBI 180 YA G 1 5 YPYDVPDYA GA 1 5 YPYDVP HA HA HA YAGYPYDVPDYAGAYPYDVP a t g c g g g c t a c c c g t a t g a c g t g c c g g a c t a t g c a g g a g c c t a t c c t t a t g a c g t t c c a g t a c g c c c g a t g g g c a t a c t g c a c g g c c t g a t a c g t c c t c g g a t a g g a a t a c t g c a a g g t c

EaeIMscIEarITatIXbaI 240 DYA (in frame with HA) GSRCTP 130 PKKKRKV APSM HA SV40 NLS DYAGSRCTPPKKKRKVAPSM a t t a c g c t g g t t c t a g a t g t a c t c c a c c a a a g a a g a a g a g a a a g g t t g c c c c t a g c a t g g t a a t g c g a c c a a g a t c t a c a t g a g g t g g t t t c t t c t t c t c t t t c c a a c g g g g a t c g t a c c

AgeIApoI 300 (in frame with SV40 NLS) ANLLTVHQNLPALPVDATSD ANLLTVHQNLPALPVDATSD c c a a t t t a c t g a c c g t a c a c c a a a a t t t g c c t g c a t t a c c g g t c g a t g c a a c g a g t g a t g g g t t a a a t g a c t g g c a t g t g g t t t t a a a c g g a c g t a a t g g c c a g c t a c g t t g c t c a c t a c

AflIIIPciI 360 (in frame with SV40 NLS) EVRKNLMDMFRDRQAFSEHT EVRKNLMDMFRDRQAFSEHT a g g t t c g c a a g a a c c t g a t g g a c a t g t t c a g g g a t c g c c a g g c g t t t t c t g a g c a t a c c t t c c a a g c g t t c t t g g a c t a c c t g t a c a a g t c c c t a g c g g t c c g c a a a a g a c t c g t a t g g a

420 (in frame with SV40 NLS) WKMLLSVCRSWAAWCKLNNR WKMLLSVCRSWAAWCKLNNR g g a a a a t g c t t c t g t c c g t t t g c c g g t c g t g g g c g g c a t g g t g c a a g t t g a a t a a c c g g a c c t t t t a c g a a g a c a g g c a a a c g g c c a g c a c c c g c c g t a c c a c g t t c a a c t t a t t g g c c t

BssHIINruIXmnIAlwNI 480 (in frame with SV40 NLS) KWFPAEPEDVRDYLLYLQAR KWFPAEPEDVRDYLLYLQAR a a t g g t t t c c c g c a g a a c c t g a a g a t g t t c g c g a t t a t c t t c t a t a t c t t c a g g c g c g c g t t a c c a a a g g g c g t c t t g g a c t t c t a c a a g c g c t a a t a g a a g a t a t a g a a g t c c g c g c g c

RsrIITaqII 540 (in frame with SV40 NLS) GLAVKTIQQHLGQLNMLHRR GLAVKTIQQHLGQLNMLHRR g t c t g g c a g t a a a a a c t a t c c a g c a a c a t t t g g g c c a g c t a a a c a t g c t t c a t c g t c g g t c a g a c c g t c a t t t t t g a t a g g t c g t t g t a a a c c c g g t c g a t t t g t a c g a a g t a g c a g c c a

BsrDI 600 (in frame with SV40 NLS) SGLPRPSDSNAVSLVMRRIR SGLPRPSDSNAVSLVMRRIR c c g g g c t g c c a c g a c c a a g t g a c a g c a a t g c t g t t t c a c t g g t t a t g c g g c g g a t c c g a a g g c c c g a c g g t g c t g g t t c a c t g t c g t t a c g a c a a a g t g a c c a a t a c g c c g c c t a g g c t t

BstBIEciIAclI 660 (in frame with SV40 NLS) KENVDAGERAKQALAFERTD KENVDAGERAKQALAFERTD a a g a a a a c g t t g a t g c c g g t g a a c g t g c a a a a c a g g c t c t a g c g t t c g a a c g c a c t g a t t t t c t t t t g c a a c t a c g g c c a c t t g c a c g t t t t g t c c g a g a t c g c a a g c t t g c g t g a c t a a

AsiSIPvuISexAI*CsiI 720 (in frame with SV40 NLS) FDQVRSLMENSDRCQDIRNL FDQVRSLMENSDRCQDIRNL t c g a c c a g g t t c g t t c a c t c a t g g a a a a t a g c g a t c g c t g c c a g g a t a t a c g t a a t c t g g a g c t g g t c c a a g c a a g t g a g t a c c t t t t a t c g c t a g c g a c g g t c c t a t a t g c a t t a g a c c

PsiI 780 (in frame with SV40 NLS) AFLGIAYNTLLRIAEIARIR AFLGIAYNTLLRIAEIARIR c a t t t c t g g g g a t t g c t t a t a a c a c c c t g t t a c g t a t a g c c g a a a t t g c c a g g a t c a g g g g t a a a g a c c c c t a a c g a a t a t t g t g g g a c a a t g c a t a t c g g c t t t a a c g g t c c t a g t c c c

EcoRV 840 (in frame with SV40 NLS) VKDISRTDGGRMLIHIGRTK VKDISRTDGGRMLIHIGRTK t t a a a g a t a t c t c a c g t a c t g a c g g t g g g a g a a t g t t a a t c c a t a t t g g c a g a a c g a a a a a a t t t c t a t a g a g t g c a t g a c t g c c a c c c t c t t a c a a t t a g g t a t a a c c g t c t t g c t t t t

PaqCI 900 (in frame with SV40 NLS) TLVSTAGVEKALSLGVTKLV TLVSTAGVEKALSLGVTKLV c g c t g g t t a g c a c c g c a g g t g t a g a g a a g g c a c t t a g c c t g g g g g t a a c t a a a c t g g t c g g c g a c c a a t c g t g g c g t c c a c a t c t c t t c c g t g a a t c g g a c c c c c a t t g a t t t g a c c a g c

BtgZIBsmBIEsp3I 960 (in frame with SV40 NLS) ERWISVSGVADDPNNYLFCR ERWISVSGVADDPNNYLFCR a g c g a t g g a t t t c c g t c t c t g g t g t a g c t g a t g a t c c g a a t a a c t a c c t g t t t t g c c g g g t c g c t a c c t a a a g g c a g a g a c c a c a t c g a c t a c t a g g c t t a t t g a t g g a c a a a a c g g c c c

1020 (in frame with SV40 NLS) VRKNGVAAPSATSQLSTRAL VRKNGVAAPSATSQLSTRAL t c a g a a a a a a t g g t g t t g c c g c g c c a t c t g c c a c c a g c c a g c t a t c a a c t c g c g c c c t g g a g t c t t t t t t a c c a c a a c g g c g c g g t a g a c g g t g g t c g g t c g a t a g t t g a g c g c g g g a c c

End (1070) Bpu10IHaeIIBspDIClaI 1070 (in frame with SV40 NLS) EGIFEATHRLIYGAKDD EGIFEATHRLIYGAKDD a a g g g a t t t t t g a a g c a a c t c a t c g a t t g a t t t a c g g c g c t a a g g a t g a c t t c c c t a a a a a c t t c g t t g a g t a g c t a a c t a a a t g c c g c g a t t c c t a c t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.