Addgene PCS4 3XFLAG-PPARgamma1 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: PCS4 3XFLAG-PPARgamma1 Sequencing Result

Map View

800 600 400 200 End (874) PvuII - MspA1I (870) ScaI (751) MscI (583) EaeI (581) BspHI (555) PspFI (540) BseYI (536) BfuAI - BspMI (513) AcuI (487) XmnI (416) BsmI (348) BglI (291) HincII (273) SmlI AvaI XhoI BsoBI PaeR7I (256) ApoI - EcoRI (243) FseI (231) NaeI (229) BsrFI - NgoMIV (227) ApaI - BanII - BaeGI - Bsp1286I - Bme1580I (143) PspOMI - EcoO109I (139) AscI - BssHII (125) BsrGI (111) ClaI - BspDI (106) SfcI (66) SP6 (54 .. 71) AatII (40) ZraI (38) BsaHI (37) MmeI (20) NspI - SphI (5) Start (0) SP6 promoter FLAG FLAG FLAG PCS4 3XFLAG-PPARgamma1 874 bp

Sequence View

BsaHIZraIAatIIMmeIStart (0) SphINspI 5ʹ 3ʹ 60 SP6 promoter A T T T A G G SP6 g c a t g c t c g t t t a g g g a a c c g c c a t t c t g c c t g g g g a c g t c g g a g c a a g c t t g a t t t a g g c g t a c g a g c a a a t c c c t t g g c g g t a a g a c g g a c c c c t g c a g c c t c g t t c g a a c t a a a t c c

BsrGIBspDIClaISfcI 120 SP6 promoter T G A C A C T A T A G SP6 t g a c a c t a t a g a a t a c a a g c t a c t t g t t c t t t t t g c a g g a t c c c a t c g a t t g t a c a g a t a a c t g t g a t a t c t t a t g t t c g a t g a a c a a g a a a a a c g t c c t a g g g t a g c t a a c a t g t c t a t

EcoO109IPspOMIBme1580IBsp1286IBaeGIBanIIApaIBssHIIAscI 180 1 5 DYKDDDDK G 1 DY FLAG FLAG MGPGDYKDDDDKGDY t c t g g c g c g c c t a c c a t g g g g c c c g g c g a c t a c a a g g a t g a c g a t g a c a a g g g c g a c t a c a g a c c g c g c g g a t g g t a c c c c g g g c c g c t g a t g t t c c t a c t g c t a c t g t t c c c g c t g a t g

NgoMIVBsrFINaeIFseI 240 5 KDDDDK G 1 5 DYKDDDDK (in frame with FLAG) GRPKI FLAG FLAG KDDDDKGDYKDDDDKGRPKI a a g g a t g a c g a t g a c a a g g g c g a c t a c a a g g a t g a c g a t g a c a a g g g c c g g c c t a a g a t c t t c c t a c t g c t a c t g t t c c c g c t g a t g t t c c t a c t g c t a c t g t t c c c g g c c g g a t t c t a g

BglIHincIIPaeR7IBsoBIXhoISmlIAvaIEcoRIApoI 300 (in frame with FLAG) WNSRPLEMTMVDTEMPFWPT WNSRPLEMTMVDTEMPFWPT t g g a a t t c a a g g c c t c t c g a g a t g a c c a t g g t t g a c a c a g a g a t g c c a t t c t g g c c c a c c a c c t t a a g t t c c g g a g a g c t c t a c t g g t a c c a a c t g t g t c t c t a c g g t a a g a c c g g g t g g

BsmI 360 (in frame with FLAG) NFGISSVDLSVMEDHSHSFD NFGISSVDLSVMEDHSHSFD a a c t t c g g a a t c a g c t c t g t g g a c c t c t c c g t g a t g g a a g a c c a c t c g c a t t c c t t t g a c t t g a a g c c t t a g t c g a g a c a c c t g g a g a g g c a c t a c c t t c t g g t g a g c g t a a g g a a a c t g

XmnI 420 (in frame with FLAG) IKPFTTVDFSSISAPHYEDI IKPFTTVDFSSISAPHYEDI a t c a a g c c c t t t a c c a c a g t t g a t t t c t c c a g c a t t t c t g c t c c a c a c t a t g a a g a c a t t t a g t t c g g g a a a t g g t g t c a a c t a a a g a g g t c g t a a a g a c g a g g t g t g a t a c t t c t g t a a

480 (in frame with FLAG) PFTRADPMVADYKYDLKLQE PFTRADPMVADYKYDLKLQE c c a t t c a c a a g a g c t g a c c c a a t g g t t g c t g a t t a c a a a t a t g a c c t g a a g c t c c a a g a a g g t a a g t g t t c t c g a c t g g g t t a c c a a c g a c t a a t g t t t a t a c t g g a c t t c g a g g t t c t t

BseYIPspFIBspMIBfuAIAcuI 540 (in frame with FLAG) YQSAIKVEPASPPYYSEKTQ YQSAIKVEPASPPYYSEKTQ t a c c a a a g t g c g a t c a a a g t a g a a c c t g c a t c t c c a c c t t a t t a t t c t g a a a a g a c c c a g a t g g t t t c a c g c t a g t t t c a t c t t g g a c g t a g a g g t g g a a t a a t a a g a c t t t t c t g g g t c

EaeIMscIBspHI 600 (in frame with FLAG) LYNRPHEEPSNSLMAIECRV LYNRPHEEPSNSLMAIECRV c t c t a c a a c a g g c c t c a t g a a g a a c c t t c t a a c t c c c t c a t g g c c a t t g a g t g c c g a g t c g a g a t g t t g t c c g g a g t a c t t c t t g g a a g a t t g a g g g a g t a c c g g t a a c t c a c g g c t c a g

660 (in frame with FLAG) CGDKASGFHYGVHACEGCKG CGDKASGFHYGVHACEGCKG t g t g g g g a t a a a g c a t c a g g c t t c c a c t a t g g a g t t c a t g c t t g t g a a g g a t g c a a g g g t a c a c c c c t a t t t c g t a g t c c g a a g g t g a t a c c t c a a g t a c g a a c a c t t c c t a c g t t c c c a

720 (in frame with FLAG) FFRRTIRLKLIYDRCDLNCR FFRRTIRLKLIYDRCDLNCR t t t t t c c g a a g a a c c a t c c g a t t g a a g c t t a t t t a t g a t a g g t g t g a t c t t a a c t g c c g g a a a a a g g c t t c t t g g t a g g c t a a c t t c g a a t a a a t a c t a t c c a c a c t a g a a t t g a c g g c c

ScaI 780 (in frame with FLAG) IHKKSRNKCQYCRFQKCLAV IHKKSRNKCQYCRFQKCLAV a t c c a c a a a a a a a g t a g a a a t a a a t g t c a g t a c t g t c g g t t t c a g a a g t g c c t t g c t g t g t a g g t g t t t t t t t c a t c t t t a t t t a c a g t c a t g a c a g c c a a a g t c t t c a c g g a a c g a c a c

840 (in frame with FLAG) GMSHNAIRFGRMPQAEKEKL GMSHNAIRFGRMPQAEKEKL g g g a t g t c t c a c a a t g c c a t c a g g t t t g g g c g g a t g c c a c a g g c c g a g a a g g a g a a g c t g c c c t a c a g a g t g t t a c g g t a g t c c a a a c c c g c c t a c g g t g t c c g g c t c t t c c t c t t c g a c

MspA1IPvuIIEnd (874) 3ʹ 5ʹ 874 (in frame with FLAG) LAEISSDIDQL LAEISSDIDQL t t g g c g g a g a t c t c c a g t g a t a t c g a c c a g c t g a a a c c g c c t c t a g a g g t c a c t a t a g c t g g t c g a c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.