Addgene pcDNA3-HA p21 K163R Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pcDNA3-HA p21 K163R Sequencing Result

Map View

800 600 400 200 End (890) BsaAI - SnaBI (887) MslI (862) TatI (842) AatII (784) ZraI (782) BsaHI (781) EciI (733) CMV-F (689 .. 709) TsoI (667) SacI - BsiHKAI (663) Eco53kI (661) BtsI BtsαI (641) T7 (596 .. 615) AseI (613) BsaI (597) HindIII (584) KpnI (582) Acc65I (578) ApoI - EcoRI (530) PspFI (516) BseYI (512) BstAPI (482) StuI (480) BglI (467) BmrI (466) EarI (459) PvuII - MspA1I (442) Esp3I - BsmBI (441) PfoI * (399) PflFI - Tth111I (371) BtgZI (369) PstI (225) BfuAI - PaqCI - BspMI (222) BbsI (196) EcoNI (163) Bpu10I (160) KflI - PpuMI (140) AcuI (130) NaeI (63) NgoMIV (61) SmlI - AvaI - XhoI - PspXI - BsoBI - PaeR7I (29) NspI - SphI (28) NsiI (26) XbaI (17) Start (0) p21 HA T7 promoter CMV promoter pcDNA3-HA p21 K163R 890 bp

Sequence View

NsiISphINspIPaeR7IBsoBIPspXIXhoISmlIAvaIXbaIStart (0) 5ʹ 3ʹ 60 165 160 *PRRKSFIL p21 *PRRKSFIL c t a t a g a a t a g g g c c c t c t a g a t g c a t g c t c g a g t t a g g g c c t c c t c t t g g a g a a g a t c a g a t a t c t t a t c c c g g g a g a t c t a c g t a c g a g c t c a a t c c c g g a g g a g a a c c t c t t c t a g t

NgoMIVNaeI 120 155 150 145 140 RRKSHYFDTMSTQRRKRGQS p21 RRKSHYFDTMSTQRRKRGQS g c c g g c g t t t g g a g t g g t a g a a a t c t g t c a t g c t g g t c t g c c g c c g t t t t c g a c c c t g a g c g g c c g c a a a c c t c a c c a t c t t t a g a c a g t a c g a c c a g a c g g c g g c a a a a g c t g g g a c t c

Bpu10IEcoNIPpuMIKflIAcuI 180 135 130 125 120 DGPGGPSGEAQEGSRPVLTC p21 DGPGGPSGEAQEGSRPVLTC a g t c t c c a g g t c c a c c t g g g g a c c c t t c a g c c t g c t c c c c t g a g c g a g g c a c a a g g g t a c t c a g a g g t c c a g g t g g a c c c c t g g g a a g t c g g a c g a g g g g a c t c g c t c c g t g t t c c c a t g

BspMIPaqCIBfuAIPstIBbsI 240 115 110 105 100 SLSLDVHDEEATGQLLAPST p21 SLSLDVHDEEATGQLLAPST a a g a c a g t g a c a g g t c c a c a t g g t c t t c c t c t g c t g t c c c c t g c a g c a g a g c a g g t g a g g t t c t g t c a c t g t c c a g g t g t a c c a g a a g g a g a c g a c a g g g g a c g t c g t c t c g t c c a c t c c

300 95 90 85 80 GPRRGGGLEDRGRRPGTPLY p21 GPRRGGGLEDRGRRPGTPLY t g c c a g g c c g c c t g c c t c c t c c c a a c t c a t c c c g g c c t c g c c g g g g c c c c g t g g g a a g g t a c g g t c c g g c g g a c g g a g g a g g g t t g a g t a g g g c c g g a g c g g c c c c g g g g c a c c c t t c c a

360 75 70 65 60 LKPLGLGRVREWAFDGELPT p21 LKPLGLGRVREWAFDGELPT a g a g c t t g g g c a g g c c a a g g c c c c g c a c a c g c t c c c a g g c g a a g t c a c c c t c c a g t g g t g t c t c g a a c c c g t c c g g t t c c g g g g c g t g t g c g a g g g t c c g c t t c a g t g g g a g g t c a c c a c

PfoI*BtgZITth111IPflFI 420 55 50 45 40 ETVFDFNWRERAEQICGAML p21 ETVFDFNWRERAEQICGAML t c t c g g t g a c a a a g t c g a a g t t c c a t c g c t c a c g g g c c t c c t g g a t g c a g c c c g c c a t t a a g a g c c a c t g t t t c a g c t t c a a g g t a g c g a g t g c c c g g a g g a c c t a c g t c g g g c g g t a a t

StuIBmrIBglIEarIBsmBIEsp3IMspA1IPvuII 480 35 30 25 20 ADCDRRLQESDVPGFLRRCA p21 ADCDRRLQESDVPGFLRRCA g c g c a t c a c a g t c g c g t c t c a g c t g c t c g c t g t c c a c t g g g c c g a a g a g g c g g c g g c a g g c g c g t a g t g t c a g c g c a g a g t c g a c g a g c g a c a g g t g a c c c g g c t t c t c c g c c g c c g t c c

EcoRIApoIBseYIPspFIBstAPI 540 FEPS 15 10 5 1 KSGCPNQRVDGAPESM p21 KSGCPNQRVDGAPESMFEPS c c t t g c t g c c g c a t g g g t t c t g a c g g a c a t c c c c a g c c g g t t c t g a c a t g a a t t c g g g a g g g a a c g a c g g c g t a c c c a a g a c t g c c t g t a g g g g t c g g c c a a g a c t g t a c t t a a g c c c t c

BsaIAcc65IKpnIHindIII 600 5 1 AYDPVDYPY HA T7 promoter G G G A T T7 AYDPVDYPYSAM a g g c a t a a t c t g g c a c a t c a t a a g g g t a g g a g g c c a t g g t a c c a a g c t t g g g t c t c c c t a t c c g t a t t a g a c c g t g t a g t a t t c c c a t c c t c c g g t a c c a t g g t t c g a a c c c a g a g g g a t

BtsαIBtsIAseI 660 T7 promoter CMV promoter A T C A C T C A G C A T A A T T7 t a g t g a g t c g t a t t a a t t t c g a t a a g c c a g t a a g c a g t g g g t t c t c t a g t t a g c c a g a g a a t c a c t c a g c a t a a t t a a a g c t a t t c g g t c a t t c g t c a c c c a a g a g a t c a a t c g g t c t c t

Eco53kIBsiHKAISacITsoI 720 CMV promoter G T G C G G A T G G C G G G T A A A C G C CMV-F g c t c t g c t t a t a t a g a c c t c c c a c c g t a c a c g c c t a c c g c c c a t t t g c g t c a a t g g g g c g c g a g a c g a a t a t a t c t g g a g g g t g g c a t g t g c g g a t g g c g g g t a a a c g c a g t t a c c c c g c

EciI 780 CMV promoter g a g t t g t t a c g a c a t t t t g g a a a g t c c c g t t g a t t t t g g t g c c a a a a c a a a c t c c c a t t g c t c a a c a a t g c t g t a a a a c c t t t c a g g g c a a c t a a a a c c a c g g t t t t g t t t g a g g g t a a c

BsaHIZraIAatII 840 CMV promoter a c g t c a a t g g g g t g g a g a c t t g g a a a t c c c c g t g a g t c a a a c c g c t a t c c a c g c c c a t t g t g c a g t t a c c c c a c c t c t g a a c c t t t a g g g g c a c t c a g t t t g g c g a t a g g t g c g g g t a a c

SnaBIBsaAIEnd (890) MslITatI 3ʹ 5ʹ 890 CMV promoter a t g t a c t g c c a a a a c c g c a t c a c c a t g g t a a t a g c g a t g a c t a a t a c g t a t a c a t g a c g g t t t t g g c g t a g t g g t a c c a t t a t c g c t a c t g a t t a t g c a t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.