Addgene pNR239 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pNR239


Map View

AfeI (8080) BstXI (7565) PflFI - Tth111I (7374) BtsI - BtsαI (7089) BclI * (6844) AleI (6103) SpeI (5920) PfoI * (5890) ApaI - BanII (5678) PspOMI (5674) PciI (5160) RBS GFP-R (4788 .. 4816) PmlI (4521) HpaI (4357) BstBI (4222) GFP-F (4173 .. 4193) HindIII (4124) BamHI (4094) BlpI (8164) SbfI (439) FspI - FspAI (708) EcoNI (990) XbaI (1264) StuI (1286) BglI (1292) XcmI (1774) BciVI - MreI (1964) FseI (1975) SacII (1979) BstAPI - DraIII (2518) NruI (2816) CAT-R (3091 .. 3110) ScaI (3655) AvrII (3721) SphI (3761) NsiI (3763) PacI (3836) BspDI * - ClaI * (3898) BsiWI (3903) KasI (3958) BsaHI - NarI (3959) SfoI (3960) PluTI (3962) MluI (4082) pNR239 8186 bp

Sequence View

5ʹ 3ʹ 60 incC c c g t g g g a a a a a c t c c a g g t a g a g g t a c a c a c g c g g a t a g c c a a t t c a g a g t a a t a a a c t g g c a c c c t t t t t g a g g t c c a t c t c c a t g t g t g c g c c t a t c g g t t a a g t c t c a t t a t t t g a

120 incC g t g a t a a t c a a c c c t c a t c a a t g a t g a c g a a c t a a c c c c c g a t a t c a g g t c a c a t g a c g a c a c t a t t a g t t g g g a g t a g t t a c t a c t g c t t g a t t g g g g g c t a t a g t c c a g t g t a c t g c t

180 incC a g g g a a a g a g a a g g a a a t c a a c t g t g a c a a a c t g c c c t c a a a t t t g g c t t c c t t a a a a a t t c c c t t t c t c t t c c t t t a g t t g a c a c t g t t t g a c g g g a g t t t a a a c c g a a g g a a t t t t t a

240 incC t a c a g t t c a a a a a g t a t g a g a a a a t c c a t g c a g g c t g a a g g a a a c a g c a a a a c t g t g a c a a t g t c a a g t t t t t c a t a c t c t t t t a g g t a c g t c c g a c t t c c t t t g t c g t t t t g a c a c t g t

300 incC a a t t a c c c t c a g t a g g t c a g a a c a a a t g t g a c g a a c c a c c c t c a a a t c t g t g a c a g a t a a t t a a t g g g a g t c a t c c a g t c t t g t t t a c a c t g c t t g g t g g g a g t t t a g a c a c t g t c t a t t

360 250 245 240 *GTTMSTIDRFSFVIHT incC repE *GTTMSTIDRFSFVIHT c c c t c a g a c t a t c c t g t c g t c a t g g a a g t g a t a t c g c g g a a g g a a a a t a c g a t a t g a g t c g g g a g t c t g a t a g g a c a g c a g t a c c t t c a c t a t a g c g c c t t c c t t t t a t g c t a t a c t c a g

420 235 230 225 220 TQRGKKKEIYSLRMPTRSNI repE TQRGKKKEIYSLRMPTRSNI g t c t g g c g g c c t t t c t t t t t c t c a a t g t a t g a g a g g c g c a t t g g a g t t c t g c t g t t g a t c c a g a c c g c c g g a a a g a a a a a g a g t t a c a t a c t c t c c g c g t a a c c t c a a g a c g a c a a c t a g

SbfI 480 215 210 205 200 ENVCVQLFRRRFDPMRQYSQ repE ENVCVQLFRRRFDPMRQYSQ t c a t t a a c a c a g a c c t g c a g g a a g c g g c g g c g g a a g t c a g g c a t a c g c t g g t a a c t t t g a a g t a a t t g t g t c t g g a c g t c c t t c g c c g c c g c c t t c a g t c c g t a t g c g a c c a t t g a a a c t

540 195 190 185 180 PLQYREIIWDIKLSVIGSGD repE PLQYREIIWDIKLSVIGSGD g g c a g c t g g t a a c g c t c t a t g a t c c a g t c g a t t t t c a g a g a g a c g a t g c c t g a g c c a t c c c c g t c g a c c a t t g c g a g a t a c t a g g t c a g c t a a a a g t c t c t c t g c t a c g g a c t c g g t a g g

600 175 170 165 160 PKRYQCLSEYLRMAYPNTIE repE PKRYQCLSEYLRMAYPNTIE g g c t t a c g a t a c t g a c a c a g g g a t t c g t a t a a a c g c a t g g c a t a c g g a t t g g t g a t t t c t c c g a a t g c t a t g a c t g t g t c c c t a a g c a t a t t t g c g t a c c g t a t g c c t a a c c a c t a a a g a

660 155 150 145 140 KTESLRFQTFRNQLGIFFPI repE KTESLRFQTFRNQLGIFFPI t t t g t t t c a c t a a g c c g a a a c t g c g t a a a c c g g t t c t g t a a c c c g a t a a a g a a g g g a a t g a a a c a a a g t g a t t c g g c t t t g a c g c a t t t g g c c a a g a c a t t g g g c t a t t t c t t c c c t t a c

FspAIFspI 720 135 130 125 120 LYPNIHVSYLGRSPSHARKI repE LYPNIHVSYLGRSPSHARKI a g a t a t g g g t t g a t a t g t a c a c t g t a a a g c c c t c t g g a t g g a c t g t g c g c a c g t t t g a t a t c t a t a c c c a a c t a t a c a t g t g a c a t t t c g g g a g a c c t a c c t g a c a c g c g t g c a a a c t a t

780 115 110 105 100 FWPFSEYGKEDGADEEPRYF repE FWPFSEYGKEDGADEEPRYF a a c c a a g g a a a a g a t t c a t a g c c t t t t t c a t c g c c g g c a t c c t c t t c a g g g c g a t a a a a a t t g g t t c c t t t t c t a a g t a t c g g a a a a a g t a g c g g c c g t a g g a g a a g t c c c g c t a t t t t t

840 95 90 85 80 VVEKGAFSKLAQRIDKSAEA repE VVEKGAFSKLAQRIDKSAEA a c c a c t t c c t t c c c c g c g a a a c t c t t c a a t g c c t g c c g t a t a t c c t t a c t g g c t t c c g c a t g g t g a a g g a a g g g g c g c t t t g a g a a g t t a c g g a c g g c a t a t a g g a a t g a c c g a a g g c g t

900 75 70 65 60 STLGFIEAYKAVHIECIGDH repE STLGFIEAYKAVHIECIGDH g a g g t c a a t c c g a a t a t t t c a g c a t a t t t a g c a a c a t g g a t c t c g c a g a t a c c g t c a t g t c t c c a g t t a g g c t t a t a a a g t c g t a t a a a t c g t t g t a c c t a g a g c g t c t a t g g c a g t a c a

960 55 50 45 40 EQLTGDSKRIQDVFLYLMRK repE EQLTGDSKRIQDVFLYLMRK t c c t g t a g g g t g c c a t c a g a t t t t c t g a t c t g g t c a a c g a a c a g a t a c a g c a t a c g t t t t a g g a c a t c c c a c g g t a g t c t a a a a g a c t a g a c c a g t t g c t t g t c t a t g t c g t a t g c a a a a

EcoNI 1020 35 30 25 20 QDRSLSYAAETLDNSQVIRP repE QDRSLSYAAETLDNSQVIRP t g a t c c c g g g a g a g a c t a t a t g c c g c c t c a g t g a g g t c g t t t g a c t g g a c g a t t c g c g g g a c t a g g g c c c t c t c t g a t a t a c g g c g g a g t c a c t c c a g c a a a c t g a c c t g c t a a g c g c c c

1080 15 10 5 1 SNKRKKHNIVATEAM repE SNKRKKHNIVATEAM c t a t t t t t a c g t t t c t t g t g a t t g a t a a c c g c t g t t t c c g c c a t g a c a g a t c c a t g t g a a g a t a a a a a t g c a a a g a a c a c t a a c t a t t g g c g a c a a a g g c g g t a c t g t c t a g g t a c a c t t

1140 g t g t g a c a a g t t t t t a g a t t g t c a c a c t a a a t a a a a a a g a g t c a a t a a g c a g g g a t a a c t c a c a c t g t t c a a a a a t c t a a c a g t g t g a t t t a t t t t t t c t c a g t t a t t c g t c c c t a t t g a

1200 ori2 t t g t g a a a a a a c a g c t t c t t c t g a g g g c a a t t t g t c a c a g g g t t a a g g g c a a t t t g t c a c a a c a c t t t t t t g t c g a a g a a g a c t c c c g t t a a a c a g t g t c c c a a t t c c c g t t a a a c a g t g

1260 ori2 a g a c a g g a c t g t c a t t t g a g g g t g a t t t g t c a c a c t g a a a g g g c a a t t t g t c a c a a c a c c t c t g t c c t g a c a g t a a a c t c c c a c t a a a c a g t g t g a c t t t c c c g t t a a a c a g t g t t g t g g

BglIStuIXbaI 1320 ori2 t t c t c t a g a a c c a g c a t g g a t a a a g g c c t a c a a g g c g c t c t a a a a a a g a a g a t c t a a a a a a a g a g a t c t t g g t c g t a c c t a t t t c c g g a t g t t c c g c g a g a t t t t t t c t t c t a g a t t t t t

1380 ori2 c t a t a a a a a a a a t a a t t a t a a a a a t a t c c c c g t g g a t a a g t g g a t a a c c c c a a g g g a a g t g a t a t t t t t t t t a t t a a t a t t t t t a t a g g g g c a c c t a t t c a c c t a t t g g g g t t c c c t t c a

1440 t t t t t c a g g c a t c g t g t g t a a g c a g a a t a t a t a a g t g c t g t t c c c t g g t g c t t c c t c g c t a a a a a g t c c g t a g c a c a c a t t c g t c t t a t a t a t t c a c g a c a a g g g a c c a c g a a g g a g c g a

1500 oriV oriV c a c t c g a c c g g g a g g g t t c g a g a a g g g g g g g c a c c c c c c t t c g g c g t g c g c g g t c a c g c g g t g a g c t g g c c c t c c c a a g c t c t t c c c c c c c g t g g g g g g a a g c c g c a c g c g c c a g t g c g c

1560 oriV oriV c a c a g g g c g c a g c c c t g g t t a a a a a c a a g g t t t a t a a a t a t t g g t t t a a a a g c a g g t t a a g t g t c c c g c g t c g g g a c c a a t t t t t g t t c c a a a t a t t t a t a a c c a a a t t t t c g t c c a a t t

1620 oriV oriV a a g a c a g g t t a g c g g t g g c c g a a a a a c g g g c g g a a a c c c t t g c a a a t g c t g g a t t t t c t g t t c t g t c c a a t c g c c a c c g g c t t t t t g c c c g c c t t t g g g a a c g t t t a c g a c c t a a a a g a c

1680 oriV oriV c c t g t g g a c a g c c c c t c a a a t g t c a a t a g g t g c g c c c c t c a t c t g t c a g c a c t c t g c c c c g g a c a c c t g t c g g g g a g t t t a c a g t t a t c c a c g c g g g g a g t a g a c a g t c g t g a g a c g g g g

1740 oriV oriV t c a a g t g t c a a g g a t c g c g c c c c t c a t c t g t c a g t a g t c g c g c c c c t c a a g t g t c a a t a c a g t t c a c a g t t c c t a g c g c g g g g a g t a g a c a g t c a t c a g c g c g g g g a g t t c a c a g t t a t g

XcmI 1800 oriV oriV c g c a g g g c a c t t a t c c c c a g g c t t g t c c a c a t c a t c t g t g g g a a a c t c g c g t a a a a t c a g g c g t c c c g t g a a t a g g g g t c c g a a c a g g t g t a g t a g a c a c c c t t t g a g c g c a t t t t a g t c

1860 oriV oriV g c g t t t t c g c c g a t t t g c g a g g c t g g c c a g c t c c a c g t c g c c g g c c g a a a t c g a g c c t g c c g c a a a a g c g g c t a a a c g c t c c g a c c g g t c g a g g t g c a g c g g c c g g c t t t a g c t c g g a c g

1920 oriV oriV c c c t c a t c t g t c a a c g c c g c g c c g g g t g a g t c g g c c c c t c a a g t g t c a a c g t c c g c c c c t g g g a g t a g a c a g t t g c g g c g c g g c c c a c t c a g c c g g g g a g t t c a c a g t t g c a g g c g g g g a

SacIIFseIBciVIMreI 1980 oriV oriV c a t c t g t c a g t g a g g g c c a a g t t t t c c g c g a g g t a t c c a c a a c g c c g g c g g c c g g c c g c g g t a g a c a g t c a c t c c c g g t t c a a a a g g c g c t c c a t a g g t g t t g c g g c c g c c g g c c g g c g c

2040 oriV g t g t c t c g c a c a c g g c t t c g a c g g c g t t t c t g g c g c g t t t g c a g g g c c a t a g a c g g c c g c c a c a g a g c g t g t g c c g a a g c t g c c g c a a a g a c c g c g c a a a c g t c c c g g t a t c t g c c g g c g

2100 oriV c a g c c c a g c g g c g a g g g c a a c c a g c c g a g g g c t t c g c c c t g t c g c t c g a c t g c g g c g a g c g t c g g g t c g c c g c t c c c g t t g g t c g g c t c c c g a a g c g g g a c a g c g a g c t g a c g c c g c t c g

2160 a c t a c t g g c t g t a a a a g g a c a g a c c a c a t c a t g g t t c t g t g t t c a t t a g g t t g t t c t g t c t g a t g a c c g a c a t t t t c c t g t c t g g t g t a g t a c c a a g a c a c a a g t a a t c c a a c a a g a c a g

2220 c a t t g c t g a c a t a a t c c g c t c c a c t t c a a c g t a a c a c c g c a c g a a g a t t t c t a t t g t t c c g t a a c g a c t g t a t t a g g c g a g g t g a a g t t g c a t t g t g g c g t g c t t c t a a a g a t a a c a a g g

2280 t g a a g g c a t a t t c a a a t c g t t t t c g t t a c c g c t t g c a g g c a t c a t g a c a g a a c a c t a c t t a c t t c c g t a t a a g t t t a g c a a a a g c a a t g g c g a a c g t c c g t a g t a c t g t c t t g t g a t g a a

2340 c c t a t a a a c g c t a c a c a g g c t c c t g a g a t t a a t a a t g c g g a t c t c t a c g a t a a t g g g a g a g g a t a t t t g c g a t g t g t c c g a g g a c t c t a a t t a t t a c g c c t a g a g a t g c t a t t a c c c t c t

2400 t t t t c c c g a c t g t t t c g t t c g c t t c t c a g t g g a t a a c a g c c a g c t t c t c t g t t t a a c a g a a a a a g g g c t g a c a a a g c a a g c g a a g a g t c a c c t a t t g t c g g t c g a a g a g a c a a a t t g t c t

2460 c a a a a a c a g c a t a t c c a c t c a g t t c c a c a t t t c c a t a t a a a g g c c a a g g c a t t t a t t c t c g t t t t t g t c g t a t a g g t g a g t c a a g g t g t a a a g g t a t a t t t c c g g t t c c g t a a a t a a g a g

BstAPIDraIII 2520 a g g a t a a t t g t t t c a g c a t c g c a a c c g c a t c a g a c t c c g g c a t c g c a a a c t g c a c c c g g t t c c t a t t a a c a a a g t c g t a g c g t t g g c g t a g t c t g a g g c c g t a g c g t t t g a c g t g g g c c a

2580 g c c g g g c a g c c a c a t c c a g c g c a a a a a c c t t c g t g t a g a c t t c c g t t g a a c t g a t g g a c t c g g c c c g t c g g t g t a g g t c g c g t t t t t g g a a g c a c a t c t g a a g g c a a c t t g a c t a c c t g a

2640 t a t g t c c c a t c a g g c t t t g c a g a a c t t t c a g c g g t a t a c c g g c a t a c a g c a t g t g c a t c g a t a c a g g g t a g t c c g a a a c g t c t t g a a a g t c g c c a t a t g g c c g t a t g t c g t a c a c g t a g c

2700 c a t a g g a a t g g c g g a a c g t a t g t g g t g t g a c c g g a a c a g a g a a c g t c a c a c c g t c a g c a g g t a t c c t t a c c g c c t t g c a t a c a c c a c a c t g g c c t t g t c t c t t g c a g t g t g g c a g t c g t c

2760 c a g c g g c g g c a a c c g c c t c c c c a a t c c a g g t c c t g a c c g t t c t g t c c g t c a c t t c c c a g a g t c g c c g c c g t t g g c g g a g g g g t t a g g t c c a g g a c t g g c a a g a c a g g c a g t g a a g g g t c t

NruI 2820 t c c g c g c t t t c t c t g t c c t t c c t g t g c g a c g g t t a c g c c g c t c c a t g a g c t t a t c g c g a a a g g c g c g a a a g a g a c a g g a a g g a c a c g c t g c c a a t g c g g c g a g g t a c t c g a a t a g c g c t t

2880 t a a a t a c c t g t g a c g g a a g a t c a c t t c g c a g a a t a a a t a a a t c c t g g t g t c c c t g t t g a t a t t t a t g g a c a c t g c c t t c t a g t g a a g c g t c t t a t t t a t t t a g g a c c a c a g g g a c a a c t a

2940 cat promoter a c c g g g a a g c c c t g g g c c a a c t t t t g g c g a a a a t g a g a c g t t g a t c g g c a c g t a a g a g g t t g g c c c t t c g g g a c c c g g t t g a a a a c c g c t t t t a c t c t g c a a c t a g c c g t g c a t t c t c c a

3000 cat promoter t c c a a c t t t c a c c a t a a t g a a a t a a g a t c a c t a c c g g g c g t a t t t t t t g a g t t a t c g a g a a g g t t g a a a g t g g t a t t a c t t t a t t c t a g t g a t g g c c c g c a t a a a a a a c t c a a t a g c t c t

3060 1 5 10 MEKKITGYTTVD cat promoter CmR MEKKITGYTTVD t t t t c a g g a g c t a a g g a a g c t a a a a t g g a g a a a a a a a t c a c t g g a t a t a c c a c c g t t g a t a a a a g t c c t c g a t t c c t t c g a t t t t a c c t c t t t t t t t a g t g a c c t a t a t g g t g g c a a c t a

3120 15 20 25 30 ISQWHRKEHFEAFQSVAQCT CmR C T C C G T A A A G T C A G T C A A C G CAT-R ISQWHRKEHFEAFQSVAQCT a t a t c c c a a t g g c a t c g t a a a g a a c a t t t t g a g g c a t t t c a g t c a g t t g c t c a a t g t a c c t a t a g g g t t a c c g t a g c a t t t c t t g t a a a a c t c c g t a a a g t c a g t c a a c g a g t t a c a t g g

3180 35 40 45 50 YNQTVQLDITAFLKTVKKNK CmR YNQTVQLDITAFLKTVKKNK t a t a a c c a g a c c g t t c a g c t g g a t a t t a c g g c c t t t t t a a a g a c c g t a a a g a a a a a t a a g a t a t t g g t c t g g c a a g t c g a c c t a t a a t g c c g g a a a a a t t t c t g g c a t t t c t t t t t a t t c

3240 55 60 65 70 HKFYPAFIHILARLMNAHPE CmR HKFYPAFIHILARLMNAHPE c a c a a g t t t t a t c c g g c c t t t a t t c a c a t t c t t g c c c g c c t g a t g a a t g c t c a t c c g g a a g t g t t c a a a a t a g g c c g g a a a t a a g t g t a a g a a c g g g c g g a c t a c t t a c g a g t a g g c c t t

3300 75 80 85 90 FRMAMKDGELVIWDSVHPCY CmR FRMAMKDGELVIWDSVHPCY t t t c g t a t g g c a a t g a a a g a c g g t g a g c t g g t g a t a t g g g a t a g t g t t c a c c c t t g t t a c a a a g c a t a c c g t t a c t t t c t g c c a c t c g a c c a c t a t a c c c t a t c a c a a g t g g g a a c a a t g

3360 95 100 105 110 TVFHEQTETFSSLWSEYHDD CmR TVFHEQTETFSSLWSEYHDD a c c g t t t t c c a t g a g c a a a c t g a a a c g t t t t c a t c g c t c t g g a g t g a a t a c c a c g a c g a t t g g c a a a a g g t a c t c g t t t g a c t t t g c a a a a g t a g c g a g a c c t c a c t t a t g g t g c t g c t a

3420 115 120 125 130 FRQFLHIYSQDVACYGENLA CmR FRQFLHIYSQDVACYGENLA t t c c g g c a g t t t c t a c a c a t a t a t t c g c a a g a t g t g g c g t g t t a c g g t g a a a a c c t g g c c a a g g c c g t c a a a g a t g t g t a t a t a a g c g t t c t a c a c c g c a c a a t g c c a c t t t t g g a c c g g

3480 135 140 145 150 YFPKGFIENMFFVSANPWVS CmR YFPKGFIENMFFVSANPWVS t a t t t c c c t a a a g g g t t t a t t g a g a a t a t g t t t t t c g t c t c a g c c a a t c c c t g g g t g a g t a t a a a g g g a t t t c c c a a a t a a c t c t t a t a c a a a a a g c a g a g t c g g t t a g g g a c c c a c t c a

3540 155 160 165 170 FTSFDLNVANMDNFFAPVFT CmR FTSFDLNVANMDNFFAPVFT t t c a c c a g t t t t g a t t t a a a c g t g g c c a a t a t g g a c a a c t t c t t c g c c c c c g t t t t c a c c a a g t g g t c a a a a c t a a a t t t g c a c c g g t t a t a c c t g t t g a a g a a g c g g g g g c a a a a g t g g

3600 175 180 185 190 MGKYYTQGDKVLMPLAIQVH CmR MGKYYTQGDKVLMPLAIQVH a t g g g c a a a t a t t a t a c g c a a g g c g a c a a g g t g c t g a t g c c g c t g g c g a t t c a g g t t c a t t a c c c g t t t a t a a t a t g c g t t c c g c t g t t c c a c g a c t a c g g c g a c c g c t a a g t c c a a g t a

ScaI 3660 195 200 205 210 HAVCDGFHVGRMLNELQQYC CmR HAVCDGFHVGRMLNELQQYC c a t g c c g t t t g t g a t g g c t t c c a t g t c g g c a g a a t g c t t a a t g a a t t a c a a c a g t a c t g c g t a c g g c a a a c a c t a c c g a a g g t a c a g c c g t c t t a c g a a t t a c t t a a t g t t g t c a t g a c g

3720 215 220 DEWQGGA* CmR DEWQGGA* g a t g a g t g g c a g g g c g g g g c g t a a g a c c a c a g c t a a c a c c a c g t c g t c c c t a t c t g c t g c c t a c t c a c c g t c c c g c c c c g c a t t c t g g t g t c g a t t g t g g t g c a g c a g g g a t a g a c g a c g

SphINsiIAvrII 3780 c c t a g g t c t a t g a g t g g t t g c t g g a t a a c t t t a c g g g c a t g c a t a a g g c t c g t a t a a t a t g g a t c c a g a t a c t c a c c a a c g a c c t a t t g a a a t g c c c g t a c g t a t t c c g a g c a t a t t a t a

PacI 3840 a t t c a g g g a g a c c a c a a c g g t t t c c c t c t a c a a a t a a t t t t g t t t a a c t t t t t a a t t a a c t a a g t c c c t c t g g t g t t g c c a a a g g g a g a t g t t t a t t a a a a c a a a t t g a a a a a t t a a t t g

BspDI*ClaI* 3900 g g c c g a a c t t t t c g g a t c a a g c t a t g a a g g a c g c a a a g a g g g a a c t a a a c a c t t g c a t c g c c g g c t t g a a a a g c c t a g t t c g a t a c t t c c t g c g t t t c t c c c t t g a t t t g t g a a c g t a g c

KasIBsaHINarISfoIBsiWI 3960 a t c g t a c g t c c g g c a a t t a a a a a a g c g g c t a a c c a c g c c g c t t t t t t t a c g t c t g c a g g c t a g c a t g c a g g c c g t t a a t t t t t t c g c c g a t t g g t g c g g c g a a a a a a a t g c a g a c g t c c g

PluTI 4020 rrnB T1 terminator g c c a c t c g a t t c t a g g g c g g c g g a t t t g t c c t a c t c a g g a g a g c g t t c a c c g a c a a a c a a c g g t g a g c t a a g a t c c c g c c g c c t a a a c a g g a t g a g t c c t c t c g c a a g t g g c t g t t t g t t

4080 rrnB T1 terminator c a g a t a a a a c g a a a g g c c c a g t c t t t c g a c t g a g c c t t t c g t t t t a t t t g a t g c c t c t a g g t c t a t t t t g c t t t c c g g g t c a g a a a g c t g a c t c g g a a a g c a a a a t a a a c t a c g g a g a t c

HindIIIBamHIMluI 4140 *KYL c a c g c g t a c c a t g g g a t c c c c c g g g c t g c a g g a a t t c g a t a t c a a g c t t t t a t t t g t a g a g t g c g c a t g g t a c c c t a g g g g g c c c g a c g t c c t t a a g c t a t a g t t c g a a a a t a a a c a t c t

4200 C A A T G T T T G A G T T C T T C C T G G GFP-F DDMGHTIGAATVFELLVMHD g a t c a t c c a t g c c a t g t g t a a t c c c a g c a g c t g t t a c a a a c t c a a g a a g g a c c a t g t g g t c t a g t a g g t a c g g t a c a c a t t a g g g t c g t c g a c a a t g t t t g a g t t c t t c c t g g t a c a c c a

BstBI 4260 RKENPDKSLASQTSLYHNDP c t c t c t t t t c g t t g g g a t c t t t c g a a a g g g c a g a t t g t g t g g a c a g g t a a t g g t t g t c t g g a g a g a a a a g c a a c c c t a g a a a g c t t t c c c g t c t a a c a c a c c t g t c c a t t a c c a a c a g a c

4320 LLVPGDGIPTNQQYHDALQV g t a a a a g g a c a g g g c c a t c g c c a a t t g g a g t a t t t t g t t g a t a a t g g t c t g c t a g t t g a a c a t t t t c c t g t c c c g g t a g c g g t t a a c c t c a t a a a a c a a c t a t t a c c a g a c g a t c a a c t t

HpaI 4380 SGDEINHRIKFNVKIGNKQK c g c t t c c a t c t t c a a t g t t g t g t c t a a t t t t g a a g t t a a c t t t g a t t c c a t t c t t t t g t t g c g a a g g t a g a a g t t a c a a c a c a g a t t a a a a c t t c a a t t g a a a c t a a g g t a a g a a a a c a a

4440 DAMIYVNHSNYNYELKHGLI t g t c t g c c a t g a t g t a t a c a t t g t g t g a g t t a t a g t t g t a t t c c a a t t t g t g t c c a a g a a a c a g a c g g t a c t a c a t a t g t a a c a c a c t c a a t a t c a a c a t a a g g t t a a a c a c a g g t t c t t

4500 NGDEKFDIGKLEIRNVLTDG t g t t t c c a t c t t c t t t a a a a t c a a t a c c t t t t a a c t c g a t t c t a t t a a c a a g g g t a t c a c a c a a a g g t a g a a g a a a t t t t a g t t a t g g a a a a t t g a g c t a a g a t a a t t g t t c c c a t a g t g

PmlI 4560 EFKVEARTKYNGDDKFFITR c t t c a a a c t t g a c t t c a g c a c g t g t c t t g t a g t t c c c g t c a t c t t t g a a a a a t a t a g t t c g a a g t t t g a a c t g a a g t c g t g c a c a g a a c a t c a a g g g c a g t a g a a a c t t t t t a t a t c a a g

4620 EQVYGEPMASKFFDHQKMHD t t t c c t g t a c a t a a c c t t c g g g c a t g g c a c t c t t g a a a a a g t c a t g c t g t t t c a t a t g a t a a a g g a c a t g t a t t g g a a g c c c g t a c c g t g a g a a c t t t t t c a g t a c g a c a a a g t a t a c t a

4680 PYRAFCQVGYGFTTVLTPWP c t g g g t a t c t c g c a a a g c a t t g a a c a c c a t a a c c g a a a g t a g t g a c a a g t g t t g g c c a t g g a c c c a t a g a g c g t t t c g t a a c t t g t g g t a t t g g c t t t c a t c a c t g t t c a c a a c c g g t a c

4740 VPLKGTTCIFKLTLKGYTAD g a a c a g g t a g t t t t c c a g t a g t g c a a a t a a a t t t a a g g g t a a g t t t t c c g t a t g t t g c a t c t t g t c c a t c a a a a g g t c a t c a c g t t t a t t t a a a t t c c c a t t c a a a a g g c a t a c a a c g t a

4800 C C A T C T A A T T C A A GFP-R GEGEGSVSFKHGNVDGDLEV c a c c t t c a c c c t c t c c a c t g a c a g a a a a t t t g t g c c c a t t a a c a t c a c c a t c t a a t t c a a g t g g a a g t g g g a g a g g t g a c t g t c t t t t a a a c a c g g g t a a t t g t a g t g g t a g a t t a a g t t

4860 RBS C A A G A A T T G G G A C A A C GFP-R LIPVVGTFLEEGKSM c a a g a a t t g g g a c a a c t c c a g t g a a a a g t t c t t c t c c t t t a c t c a t g g t a c c t t t c t c c t g t t c t t a a c c c t g t t g a g g t c a c t t t t c a a g a a g a g g a a a t g a g t a c c a t g g a a a g a g g a

4920 RBS sTRSV HHRz c t t t a a t g a a t t c t t a a a c a a a a t t a t t t g t a g a g g c t g t t t c g t c c t c a c g g a c t c a t c g a a a t t a c t t a a g a a t t t g t t t t a a t a a a c a t c t c c g a c a a a g c a g g a g t g c c t g a g t a g

4980 sTRSV HHRz a g a c c g g a a a g c a c a t c c g g t g a c a g c t g t g c a t t t a g t t t c t c g t t t c t t c t t c t t c c a t c t g g c c t t t c g t g t a g g c c a c t g t c g a c a c g t a a a t c a a a g a g c a a a g a a g a a g a a g g t

5040 a c g a g a g a a a a c g a g g a a c t a a g c t a g c c c g c c a c c t a a g t t a t g a g t c a g g a a a a a a g g t g c t c t c t t t t g c t c c t t g a t t c g a t c g g g c g g t g g a t t c a a t a c t c a g t c c t t t t t t c c

5100 c g a c a g a g t a a t c t g t c g c c t t t t t t c t t t g c t t g c t t t g t a a t c a c t c g a t g a c c a a a a g c t g t c t c a t t a g a c a g c g g a a a a a a g a a a c g a a c g a a a c a t t a g t g a g c t a c t g g t t t t

5160 g t a a a a a c c c g c c g a a g c g g g t t t t t a c g t a a a a c a g g t g a a a c t g a c g c t a g c c g g t c a c a t t t t t g g g c g g c t t c g c c c a a a a a t g c a t t t t g t c c a c t t t g a c t g c g a t c g g c c a g t

PciI 5220 c a t g t c a a t a c t t g c c c t t g a c a g g c a t t g a t g g a a t c g t a g t c t c a c g c t g a t a g t c t g g t a c a g t t a t g a a c g g g a a c t g t c c g t a a c t a c c t t a g c a t c a g a g t g c g a c t a t c a g a c

5280 sopC a t c g a c a a t a c a a g t g g g a c c g t g g t c c c a g a c c g a t a a t c a g a c c g a c a a c a c g a g t g g t a g c t g t t a t g t t c a c c c t g g c a c c a g g g t c t g g c t a t t a g t c t g g c t g t t g t g c t c a c c

5340 sopC g a t c g t g g t c c c a g a c t a a t a a t c a g a c c g a c g a t a c g a g t g g g a c c g t g g t c c c a g a c t c t a g c a c c a g g g t c t g a t t a t t a g t c t g g c t g c t a t g c t c a c c c t g g c a c c a g g g t c t g a

5400 sopC a a t a a t c a g a c c g a c g a t a c g a g t g g g a c c g t g g t t c c a g a c t a a t a a t c a g a c c g a c g a t t a t t a g t c t g g c t g c t a t g c t c a c c c t g g c a c c a a g g t c t g a t t a t t a g t c t g g c t g c t

5460 sopC t a c g a g t g g g a c c g t g g t c c c a g a c t a a t a a t c a g a c c g a c g a t a c g a g t g g g a c c a t g g a t g c t c a c c c t g g c a c c a g g g t c t g a t t a t t a g t c t g g c t g c t a t g c t c a c c c t g g t a c c

5520 sopC t c c c a g a c t a a t a a t c a g a c c g a c g a t a c g a g t g g g a c c g t g g t c c c a g t c t g a t t a t c a a g g g t c t g a t t a t t a g t c t g g c t g c t a t g c t c a c c c t g g c a c c a g g g t c a g a c t a a t a g t

5580 sopC g a c c g a c g a t a c g a g t g g g a c c g t g g t c c c a g a c t a a t a a t c a g a c c g a c g a t a c g a g t g c t g g c t g c t a t g c t c a c c c t g g c a c c a g g g t c t g a t t a t t a g t c t g g c t g c t a t g c t c a c

5640 sopC g g a c c g t g g t c c c a g a c t a a t a a t c a g a c c g a c g a t a c g a g t g g g a c c g t g g t c c c a g t c c c t g g c a c c a g g g t c t g a t t a t t a g t c t g g c t g c t a t g c t c a c c c t g g c a c c a g g g t c a g

PspOMIBanIIApaI 5700 sopC t g a t t a t c a g a c c g a c g a t a c a a g t g g a a c a g t g g g c c c a g a g a g a a t a t t c a g g c c a g t a c t a a t a g t c t g g c t g c t a t g t t c a c c t t g t c a c c c g g g t c t c t c t t a t a a g t c c g g t c a

5760 t a t g c t t t c t g g c c t g t a a c a a a g g a c a t t a a g t a a a g a c a g a t a a a c g t a g a c t a a a a c a t a c g a a a g a c c g g a c a t t g t t t c c t g t a a t t c a t t t c t g t c t a t t t g c a t c t g a t t t t g

5820 320 315 310 *PAPKELEKLIAEIKEI sopB *PAPKELEKLIAEIKEI g t g g t c g c a t c a g g g t g c t g g c t t t t c a a g t t c c t t a a g a a t g g c c t c a a t t t t c t c t a t c a c c a g c g t a g t c c c a c g a c c g a a a a g t t c a a g g a a t t c t t a c c g g a g t t a a a a g a g a t a

5880 305 300 295 290 CETPVRSRDLNLVMKDGKYL sopB CETPVRSRDLNLVMKDGKYL a c a c t c a g t t g g a a c a c g g g a c c t g t c c a g g t t a a g c a c c a t t t t a t c g c c c t t a t a c a a t g t g a g t c a a c c t t g t g c c c t g g a c a g g t c c a a t t c g t g g t a a a a t a g c g g g a a t a t g t t

SpeIPfoI* 5940 285 280 275 270 VTAGPAFQHRSSLSTRSASS sopB VTAGPAFQHRSSLSTRSASS t a c t g t c g c t c c a g g a g c a a a c t g a t g t c g t g a g c t t a a a c t a g t t c t t g a t g c a g a t g a a t g a c a g c g a g g t c c t c g t t t g a c t a c a g c a c t c g a a t t t g a t c a a g a a c t a c g t c t a c t

6000 265 260 255 250 TKLVSTLLTIVEEAEFIVGA sopB TKLVSTLLTIVEEAEFIVGA c g t t t t a a g c a c a g a a g t t a a a a g a g t g a t a a c t t c t t c a g c t t c a a a t a t c a c c c c a g c g c a a a a t t c g t g t c t t c a a t t t t c t c a c t a t t g a a g a a g t c g a a g t t t a t a g t g g g g t c g

6060 245 240 235 230 KKQEHLNSAQQKLLEEKDTF sopB KKQEHLNSAQQKLLEEKDTF t t t t t t c t g c t c a t g a a g g t t a g a t g c c t g c t g c t t a a g t a a t t c c t c t t t a t c t g t a a a a a a a a a g a c g a g t a c t t c c a a t c t a c g g a c g a c g a a t t c a t t a a g g a g a a a t a g a c a t t t

AleI 6120 225 220 215 210 AKQLADGSRASLEGPHSFLA sopB AKQLADGSRASLEGPHSFLA g g c t t t t t g a a g t g c a t c a c c t g a c c g g g c a g a t a g t t c a c c g g g g t g a g a a a a a a g a g c c c g a a a a a c t t c a c g t a g t g g a c t g g c c c g t c t a t c a a g t g g c c c c a c t c t t t t t t c t c g

6180 205 200 195 190 VVSKPLKATNICRTIIKRSI sopB VVSKPLKATNICRTIIKRSI a a c a a c t g a t t t a g g c a a t t t g g c g g t g t t g a t a c a g c g g g t a a t a a t c t t a c g t g a a a t t t g t t g a c t a a a t c c g t t a a a c c g c c a c a a c t a t g t c g c c c a t t a t t a g a a t g c a c t t t a

6240 185 180 175 170 NEADALASINGAFENQLRSA sopB NEADALASINGAFENQLRSA a t t t t c c g c a t c a g c c a g c g c a g a a a t a t t t c c a g c a a a t t c a t t c t g c a a t c g g c t t g c t a a a a g g c g t a g t c g g t c g c g t c t t t a t a a a g g t c g t t t a a g t a a g a c g t t a g c c g a a c g

6300 165 160 155 150 YRQGREYASTPRYDNGLRSL sopB YRQGREYASTPRYDNGLRSL a t a a c g c t g a c c a c g t t c a t a a g c a c t t g t t g g g c g a t a a t c g t t a c c c a a t c t g g a t a a t a t t g c g a c t g g t g c a a g t a t t c g t g a a c a a c c c g c t a t t a g c a a t g g g t t a g a c c t a t t

6360 145 140 135 130 AAMQEDDLEGVLVRYDSETL sopB AAMQEDDLEGVLVRYDSETL t g c a g c c a t c t g c t c a t c a t c c a g c t c g c c a a c c a g a a c a c g a t a a t c a c t t t c g g t a a g a c g t c g g t a g a c g a g t a g t a g g t c g a g c g g t t g g t c t t g t g c t a t t a g t g a a a g c c a t t c

6420 125 120 115 110 AAAKRRRSGDAIEIVGSVRR sopB AAAKRRRSGDAIEIVGSVRR t g c a g c a g c t t t a c g a c g g c g a c t c c c a t c g g c a a t t t c t a t g a c a c c a g a t a c t c t t c g a c g t c g t c g a a a t g c t g c c g c t g a g g g t a g c c g t t a a a g a t a c t g t g g t c t a t g a g a a g c

6480 105 100 95 90 GFAPTQQGTLLFSPILDDLA sopB GFAPTQQGTLLFSPILDDLA a c c g a a c g c c g g t g t c t g t t g a c c a g t c a g t a g a a a a g a a g g g a t g a g a t c a t c c a g t g c t g g c t t g c g g c c a c a g a c a a c t g g t c a g t c a t c t t t t c t t c c c t a c t c t a g t a g g t c a c g

6540 85 80 75 70 DETLLEQDRENGSWVRSTKE sopB DETLLEQDRENGSWVRSTKE g t c c t c a g t a a g c a g c t c c t g g t c a c g t t c a t t a c c t g a c c a t a c c c g a g a g g t c t t c t c c a g g a g t c a t t c g t c g a g g a c c a g t g c a a g t a a t g g a c t g g t a t g g g c t c t c c a g a a g a g

6600 65 60 55 50 VSDGRLVELTFKVDRGCVPL sopB VSDGRLVELTFKVDRGCVPL a a c a c t a t c a c c c c g g a g c a c t t c a a g a g t a a a c t t c a c a t c c c g a c c a c a t a c a g g c a a t t g t g a t a g t g g g g c c t c g t g a a g t t c t c a t t t g a a g t g t a g g g c t g g t g t a t g t c c g t t

6660 45 40 35 30 TIANGRAMVGVRAILSDVMP sopB TIANGRAMVGVRAILSDVMP a g t a a t g g c a t t a c c g c g a g c c a t t a c t c c t a c g c g c g c a a t t a a c g a a t c c a c c a t c g g t c a t t a c c g t a a t g g c g c t c g g t a a t g a g g a t g c g c g c g t t a a t t g c t t a g g t g g t a g c c

6720 25 20 15 10 AAPTSLSTDEVPQTNLTHKP sopB AAPTSLSTDEVPQTNLTHKP g g c a g c t g g t g t c g a t a a c g a a g t a t c t t c a a c c g g t t g a g t a t t g a g c g t a t g t t t t g g c c g t c g a c c a c a g c t a t t g c t t c a t a g a a g t t g g c c a a c t c a t a a c t c g c a t a c a a a a c c

6780 390 385 380 *RIEWRPKILRDF sopA 5 1 IVPARKM sopB IVPARKM *RIEWRPKILRDF a a t a a c a g g c g c a c g c t t c a t t a t c t a a t c t c c c a g c g t g g t t t a a t c a g a c g a t c g a a a t t a t t g t c c g c g t g c g a a g t a a t a g a t t a g a g g g t c g c a c c a a a t t a g t c t g c t a g c t t t

6840 375 370 365 360 IENCVPEWISLANRWAGTSS sopA IENCVPEWISLANRWAGTSS a t t t c a t t g c a g a c a g g t t c c c a a a t a g a a a g a g c a t t t c t c c a g g c a c c a g t t g a a g a g t a a a g t a a c g t c t g t c c a a g g g t t t a t c t t t c t c g t a a a g a g g t c c g t g g t c a a c t t c t c

BclI* 6900 355 350 345 340 RQDIAQEFVTRMRIQGKGVE sopA RQDIAQEFVTRMRIQGKGVE c g t t g a t c a a t g g c c t g t t c a a a a a c a g t t c t c a t c c g g a t c t g a c c t t t a c c a a c t t c a g c a a c t a g t t a c c g g a c a a g t t t t t g t c a a g a g t a g g c c t a g a c t g g a a a t g g t t g a a g t

6960 335 330 325 320 DTERVVNKLVMSGWADRIQE sopA DTERVVNKLVMSGWADRIQE t c c g t t t c a c g t a c a a c a t t t t t t a g a a c c a t g c t t c c c c a g g c a t c c c g a a t t t g c t c c a g g c a a a g t g c a t g t t g t a a a a a a t c t t g g t a c g a a g g g g t c c g t a g g g c t t a a a c g a g g

7020 315 310 305 300 EMWPSQSGNSNSYKTLLIRV sopA EMWPSQSGNSNSYKTLLIRV t c c a t c c a c g g g g a c t g a g a g c c a t t a c t a t t g c t g t a t t t g g t a a g c a a a a t a c g t a c a a g g t a g g t g c c c c t g a c t c t c g g t a a t g a t a a c g a c a t a a a c c a t t c g t t t t a t g c a t g t

7080 295 290 285 280 DPEFGKLDVNKLLDRLMDFF sopA DPEFGKLDVNKLLDRLMDFF t c a g g c t c g a a c c c t t t a a g a t c a a c g t t c t t g a g c a g a t c a c g a a g c a t a t c g a a a a a c a g t c c g a g c t t g g g a a a t t c t a g t t g c a a g a a c t c g t c t a g t g c t t c g t a t a g c t t t t t g

BtsαIBtsI 7140 275 270 265 260 QLASTYDFLEAPTPVILVDA sopA QLASTYDFLEAPTPVILVDA t g c a g t g c g g a g g t g t a g t c a a a c a a c t c a g c a g g c g t g g g a a c a a t c a g c a c a t c a g c a a c g t c a c g c c t c c a c a t c a g t t t g t t g a g t c g t c c g c a c c c t t g t t a g t c g t g t a g t c g t

7200 255 250 245 240 ACVVNITGIGLNPASDIVIV sopA ACVVNITGIGLNPASDIVIV g c a c a t a c g a c a t t a a t c g t g c c g a t a c c c a g g t t a g g c g c g c t g t c a a t a a c t a t g a c a c g t g t a t g c t g t a a t t a g c a c g g c t a t g g g t c c a a t c c g c g c g a c a g t t a t t g a t a c t g t

7260 235 230 225 220 DYDHAVTEIALRLMLHPDTP sopA DYDHAVTEIALRLMLHPDTP t c a t a g t c a t g a g c a a c a g t t t c a a t g g c c a g t c g g a g c a t c a g g t g t g g a t c g g t g g g c a g t a t c a g t a c t c g t t g t c a a a g t t a c c g g t c a g c c t c g t a g t c c a c a c c t a g c c a c c c g

7320 215 210 205 200 LKGEDFKGMLETEIRHLALC sopA LKGEDFKGMLETEIRHLALC a g t t t a c c t t c a t c a a a t t t g c c c a t t a a c t c a g t t t c a a t a c g g t g c a g a g c c a g a c a g t c a a a t g g a a g t a g t t t a a a c g g g t a a t t g a g t c a a a g t t a t g c c a c g t c t c g g t c t g t c

Tth111IPflFI 7380 195 190 185 180 SPIIDLGPWCTPKIAYTVDD sopA SPIIDLGPWCTPKIAYTVDD g a a g g a a t a a t g t c a a g c c c c g g c c a g c a a g t g g g c t t t a t t g c a t a a g t g a c a t c g t c c c t t c c t t a t t a c a g t t c g g g g c c g g t c g t t c a c c c g a a a t a a c g t a t t c a c t g t a g c a g g

7440 175 170 165 160 KEGLYFPLLTDEAHIHLDPV sopA KEGLYFPLLTDEAHIHLDPV t t t t c c c c a a g a t a g a a a g g c a g g a g a g t g t c t t c t g c a t g a a t a t g a a g a t c t g g t a c c a a a a g g g g t t c t a t c t t t c c g t c c t c t c a c a g a a g a c g t a c t t a t a c t t c t a g a c c a t g g

7500 155 150 145 140 WGHYMSATGQPDNGEVLLVR sopA WGHYMSATGQPDNGEVLLVR c a t c c g t g a t a c a t t g a g g c t g t t c c c t g g g g g t c g t t a c c t t c c a c g a g c a a a a c a c g t g t a g g c a c t a t g t a a c t c c g a c a a g g g a c c c c c a g c a a t g g a a g g t g c t c g t t t t g t g c a

7560 135 130 125 120 LGKLALDQALHVSVSTKYVG sopA LGKLALDQALHVSVSTKYVG a g c c c c t t c a g a g c c a g a t c c t g a g c a a g a t g a a c a g a a a c t g a g g t t t t g t a a a c g c c a t c g g g g a a g t c t c g g t c t a g g a c t c g t t c t a c t t g t c t t t g a c t c c a a a a c a t t t g c g g t

BstXI 7620 115 110 105 100 GKHAAVGIVPPFVDEARRLR sopA GKHAAVGIVPPFVDEARRLR c c t t t a t g g g c a g c a a c c c c g a t c a c c g g t g g a a a t a c g t c t t c a g c a c g t c g c a a t c g c g g a a a t a c c c g t c g t t g g g g c t a g t g g c c a c c t t t a t g c a g a a g t c g t g c a g c g t t a g c g

7680 95 90 85 80 TGFVDRMHNIQEITYGVRQE sopA TGFVDRMHNIQEITYGVRQE g t a c c a a a c a c a t c a c g c a t a t g a t t a a t t t g t t c a a t t g t a t a a c c a a c a c g t t g c t c a c a t g g t t t g t g t a g t g c g t a t a c t a a t t a a a c a a g t t a a c a t a t t g g t t g t g c a a c g a g t

7740 75 70 65 60 VRGRIEMDPHPLRGAKEADR sopA VRGRIEMDPHPLRGAKEADR a c c c g t c c t c g a a t t t c c a t a t c c g g g t g c g g t a g t c g c c c t g c t t t c t c g g c a t c t c t g t g g g c a g g a g c t t a a a g g t a t a g g c c c a c g c c a t c a g c g g g a c g a a a g a g c c g t a g a g a c

7800 55 50 45 40 IAQSSVGVLDAAEGIRWRRT sopA IAQSSVGVLDAAEGIRWRRT a t a g c c t g a g a a g a a a c c c c a a c t a a a t c c g c t g c t t c a c c t a t t c t c c a g c g c c g g g t t t a t c g g a c t c t t c t t t g g g g t t g a t t t a g g c g a c g a a g t g g a t a a g a g g t c g c g g c c c a a

7860 35 30 25 20 IKRAEPSDDNFQAIAIAKTM sopA IKRAEPSDDNFQAIAIAKTM a t t t t c c t c g c t t c c g g g c t g t c a t c a t t a a a c t g t g c a a t g g c g a t a g c c t t c g t c a t t t a a a a g g a g c g a a g g c c c g a c a g t a g t a a t t t g a c a c g t t a c c g c t a t c g g a a g c a g t a a

7920 15 10 5 1 EHGANICQNLTEMLKMRFM sopA EHGANICQNLTEMLKMRFM t c a t g a c c a g c g t t t a t g c a c t g g t t a a g t g t t t c c a t g a g t t t c a t t c t g a a c a t c c t t a g t a c t g g t c g c a a a t a c g t g a c c a a t t c a c a a a g g t a c t c a a a g t a a g a c t t g t a g g a a

7980 t a a t c a t t g c t t t g c g t t t t t t t a t t a a a t c t t g c a a t t t a c t g c a a a g c a a c a a c a a a a a t t a g t a a c g a a a c g c a a a a a a a t a a t t t a g a a c g t t a a a t g a c g t t t c g t t g t t g t t t t

8040 t c g c a a a g t c a t c a a a a a a c c g c a a a g t t g t t t a a a a t a a g a g c a a c a c t a c a a a a g g a g a g c g t t t c a g t a g t t t t t t g g c g t t t c a a c a a a t t t t a t t c t c g t t g t g a t g t t t t c c t c

AfeI 8100 a t a a g a a g a g c a c a t a c c t c a g t c a c t t a t t a t c a c t a g c g c t c g c c g c a g c c g t g t a a c t a t t c t t c t c g t g t a t g g a g t c a g t g a a t a a t a g t g a t c g c g a g c g g c g t c g g c a c a t t g

8160 c g a g c a t a g c g a g c g a a c t g g c g a g g a a g c a a a g a a g a a c t g t t c t g t c a g a t a g c t c t t g c t c g t a t c g c t c g c t t g a c c g c t c c t t c g t t t c t t c t t g a c a a g a c a g t c t a t c g a g a a

BlpI 3ʹ 5ʹ 8186 a c g c t c a g c g c a a g a a g a a a t a t c c a t g c g a g t c g c g t t c t t c t t t a t a g g t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.