Addgene pHR_Gal4UAS_tBFP_PGK_mCherry - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pHR_Gal4UAS_tBFP_PGK_mCherry

Map View

600 400 200 End (699) BmrI - HindIII (691) AlwNI (662) BtsI - BtsαI (656) MscI (655) EaeI (653) DraIII (645) PshAI (628) BsaI (616) SfcI (588) AccI (575) BsaHI (572) SmlI (559) BpuEI (544) MflI * - BstYI (535) AcuI (505) BglI (464) MspA1I (448) Esp3I - BsmBI (427) StuI (424) PspFI (420) BseYI (416) MmeI (407) NmeAIII (393) PfoI * (329) BpmI (313) BbsI (296) Bsu36I (254) PasI (219) TsoI (163) BsiHKAI (79) ApaLI (75) BtgI (53) TatI - BsrGI (38) MslI (28) NspI (27) Start (0) TagBFP pHR_Gal4UAS_tBFP_PGK_mCherry 699 bp

Sequence View

BtgIBsrGITatINspIMslIStart (0) 5ʹ 3ʹ 60 1 5 10 15 20 MSELIKENMHMKLYMEGTVD TagBFP MSELIKENMHMKLYMEGTVD HALQNLLVHVHLQVHLAGHV a t g a g c g a g c t g a t t a a g g a g a a c a t g c a c a t g a a g c t g t a c a t g g a g g g c a c c g t g g a c t a c t c g c t c g a c t a a t t c c t c t t g t a c g t g t a c t t c g a c a t g t a c c t c c c g t g g c a c c t g

ApaLIBsiHKAI 120 25 30 35 40 NHHFKCTSEGEGKPYEGTQT TagBFP NHHFKCTSEGEGKPYEGTQT VMVELACGLAFALGVLAGLG a a c c a t c a c t t c a a g t g c a c a t c c g a g g g c g a a g g c a a g c c c t a c g a g g g c a c c c a g a c c t t g g t a g t g a a g t t c a c g t g t a g g c t c c c g c t t c c g t t c g g g a t g c t c c c g t g g g t c t g g

TsoI 180 45 50 55 60 MRIKVVEGGPLPFAFDILAT TagBFP MRIKVVEGGPLPFAFDILAT HSDLHDLAAREGEGEVDQSS a t g a g a a t c a a g g t g g t c g a g g g c g g c c c t c t c c c c t t c g c c t t c g a c a t c c t g g c t a c t t a c t c t t a g t t c c a c c a g c t c c c g c c g g g a g a g g g g a a g c g g a a g c t g t a g g a c c g a t g a

PasI 240 65 70 75 80 SFLYGSKTFINHTQGIPDFF TagBFP SFLYGSKTFINHTQGIPDFF AEEVAALGEDVVGLADGVEE a g c t t c c t c t a c g g c a g c a a g a c c t t c a t c a a c c a c a c c c a g g g c a t c c c c g a c t t c t t c t c g a a g g a g a t g c c g t c g t t c t g g a a g t a g t t g g t g t g g g t c c c g t a g g g g c t g a a g a a g

BbsIBsu36I 300 85 90 95 100 KQSFPEGFTWERVTTYEDGG TagBFP KQSFPEGFTWERVTTYEDGG LLGERLAECPLSDGCVFVPA a a g c a g t c c t t c c c t g a g g g c t t c a c a t g g g a g a g a g t c a c c a c a t a c g a a g a c g g g g g c t t c g t c a g g a a g g g a c t c c c g a a g t g t a c c c t c t c t c a g t g g t g t a t g c t t c t g c c c c c g

PfoI*BpmI 360 105 110 115 120 VLTATQDTSLQDGCLIYNVK TagBFP VLTATQDTSLQDGCLIYNVK HQGSGLVGAELVAAEDVVDL g t g c t g a c c g c t a c c c a g g a c a c c a g c c t c c a g g a c g g c t g c c t c a t c t a c a a c g t c a a g c a c g a c t g g c g a t g g g t c c t g t g g t c g g a g g t c c t g c c g a c g g a g t a g a t g t t g c a g t t c

BseYIPspFIMmeINmeAIII 420 125 130 135 140 IRGVNFTSNGPVMQKKTLGW TagBFP IRGVNFTSNGPVMQKKTLGW DSPHVECGVARHHLLFCEAP a t c a g a g g g g t g a a c t t c a c a t c c a a c g g c c c t g t g a t g c a g a a g a a a a c a c t c g g c t g g t a g t c t c c c c a c t t g a a g t g t a g g t t g c c g g g a c a c t a c g t c t t c t t t t g t g a g c c g a c c

BglIMspA1IStuIBsmBIEsp3I 480 145 150 155 160 EAFTETLYPADGGLEGRNDM TagBFP EAFTETLYPADGGLEGRNDM LGEGLRQVGSVAAQFASVVH g a g g c c t t c a c c g a g a c g c t g t a c c c c g c t g a c g g c g g c c t g g a a g g c a g a a a c g a c a t g c t c c g g a a g t g g c t c t g c g a c a t g g g g c g a c t g c c g c c g g a c c t t c c g t c t t t g c t g t a c

BstYIMflI*AcuI 540 165 170 175 180 ALKLVGGSHLIANIKTTYRS TagBFP ALKLVGGSHLIANIKTTYRS GQLEHAPAMQDCVDLGCISG g c c c t g a a g c t c g t g g g c g g g a g c c a t c t g a t c g c a a a c a t c a a g a c c a c a t a t a g a t c c c g g g a c t t c g a g c a c c c g c c c t c g g t a g a c t a g c g t t t g t a g t t c t g g t g t a t a t c t a g g

SfcIBsaHIAccISmlIBpuEI 600 185 190 195 200 KKPAKNLKMPGVYYVDYRLE TagBFP KKPAKNLKMPGVYYVDYRLE LFGSLVELHRADVIHVVSQF a a g a a a c c c g c t a a g a a c c t c a a g a t g c c t g g c g t c t a c t a t g t g g a c t a c a g a c t g g a a t t c t t t g g g c g a t t c t t g g a g t t c t a c g g a c c g c a g a t g a t a c a c c t g a t g t c t g a c c t t

EaeIMscIBtsαIBtsIDraIIIPshAIBsaI 660 205 210 215 220 RIKEANNETYVEQHEVAVAR TagBFP RIKEANNETYVEQHEVAVAR SDLLGVVLGVDLLVLHCHGS a g a a t c a a g g a g g c c a a c a a c g a g a c c t a c g t c g a g c a g c a c g a g g t g g c a g t g g c c a g a t c t t a g t t c c t c c g g t t g t t g c t c t g g a t g c a g c t c g t c g t g c t c c a c c g t c a c c g g t c t

End (699) HindIIIBmrIAlwNI 3ʹ 5ʹ 699 225 230 YCDLPSKLGHKLN TagBFP YCDLPSKLGHKLN VAVERAFQPVLKI t a c t g c g a c c t c c c t a g c a a a c t g g g g c a c a a g c t t a a t a t g a c g c t g g a g g g a t c g t t t g a c c c c g t g t t c g a a t t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.