Addgene pCARCre - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pCARCre


Map View

1250 1000 750 500 250 End (1334) NotI - EaeI - EagI (1328) MfeI (1269) HpaI - HincII (1260) SV40pA-R (1204 .. 1223) BtsI - BtsαI (1186) EBV-rev (1150 .. 1169) XbaI (1097) EcoP15I (1046) BspHI (1024) SspI (1019) BpmI (988) BaeGI - Bsp1286I - Bme1580I (935) MmeI (920) PshAI (898) HaeII Bpu10I (886) ClaI - BspDI (869) Esp3I - BsmBI (746) BfuAI PaqCI BspMI (672) EcoRV (614) PvuI - AsiSI (521) CsiI - SexAI * (490) BstBI (472) AclI (433) EciI (431) BamHI (418) RsrII (364) TaqII (352) BssHII (300) NruI (277) XmnI (271) AlwNI (264) PciI - AflIII (148) AgeI (104) SP6 (8 .. 25) HindIII (1) Start (0) SP6 promoter Cre SV40 poly(A) signal pCARCre 1334 bp

Sequence View

Start (0) HindIII 60 SP6 promoter A T T T A G G T G A C A C T A T A G SP6 a a g c t t g a t t t a g g t g a c a c t a t a g a a t a c a a g c t a c t t g t t c t t t t t g c a g g a t c t c c g t t c g a a c t a a a t c c a c t g t g a t a t c t t a t g t t c g a t g a a c a a g a a a a a c g t c c t a g a g g c

AgeI 120 1 5 10 15 MSNLLTVHQNLPALPVDAT Cre MSNLLTVHQNLPALPVDAT a t a t g t c c a a t t t a c t g a c c g t a c a c c a a a a t t t g c c t g c a t t a c c g g t c g a t g c a a c g a t a t a c a g g t t a a a t g a c t g g c a t g t g g t t t t a a a c g g a c g t a a t g g c c a g c t a c g t t g c t

AflIIIPciI 180 20 25 30 35 SDEVRKNLMDMFRDRQAFSE Cre SDEVRKNLMDMFRDRQAFSE g t g a t g a g g t t c g c a a g a a c c t g a t g g a c a t g t t c a g g g a t c g c c a g g c g t t t t c t g a g c c a c t a c t c c a a g c g t t c t t g g a c t a c c t g t a c a a g t c c c t a g c g g t c c g c a a a a g a c t c g

240 40 45 50 55 HTWKMLLSVCRSWAAWCKLN Cre HTWKMLLSVCRSWAAWCKLN a t a c c t g g a a a a t g c t t c t g t c c g t t t g c c g g t c g t g g g c g g c a t g g t g c a a g t t g a a t a t a t g g a c c t t t t a c g a a g a c a g g c a a a c g g c c a g c a c c c g c c g t a c c a c g t t c a a c t t a t

NruIXmnIAlwNI 300 60 65 70 75 NRKWFPAEPEDVRDYLLYLQ Cre NRKWFPAEPEDVRDYLLYLQ a c c g g a a a t g g t t t c c c g c a g a a c c t g a a g a t g t t c g c g a t t a t c t t c t a t a t c t t c a g g t g g c c t t t a c c a a a g g g c g t c t t g g a c t t c t a c a a g c g c t a a t a g a a g a t a t a g a a g t c c

TaqIIBssHII 360 80 85 90 95 ARGLAVKTIQQHLGQLNMLH Cre ARGLAVKTIQQHLGQLNMLH c g c g c g g t c t g g c a g t a a a a a c t a t c c a g c a a c a t t t g g g c c a g c t a a a c a t g c t t c a t c g c g c g c c a g a c c g t c a t t t t t g a t a g g t c g t t g t a a a c c c g g t c g a t t t g t a c g a a g t a g

BamHIRsrII 420 100 105 110 115 RRSGLPRPSDSNAVSLVMRR Cre RRSGLPRPSDSNAVSLVMRR g t c g g t c c g g g c t g c c a c g a c c a a g t g a c a g c a a t g c t g t t t c a c t g g t t a t g c g g c g g a c a g c c a g g c c c g a c g g t g c t g g t t c a c t g t c g t t a c g a c a a a g t g a c c a a t a c g c c g c c t

BstBIEciIAclI 480 120 125 130 135 IRKENVDAGERAKQALAFER Cre IRKENVDAGERAKQALAFER t c c g a a a a g a a a a c g t t g a t g c c g g t g a a c g t g c a a a a c a g g c t c t a g c g t t c g a a c g c a a g g c t t t t c t t t t g c a a c t a c g g c c a c t t g c a c g t t t t g t c c g a g a t c g c a a g c t t g c g t

AsiSIPvuISexAI*CsiI 540 140 145 150 155 TDFDQVRSLMENSDRCQDIR Cre TDFDQVRSLMENSDRCQDIR c t g a t t t c g a c c a g g t t c g t t c a c t c a t g g a a a a t a g c g a t c g c t g c c a g g a t a t a c g t a g a c t a a a g c t g g t c c a a g c a a g t g a g t a c c t t t t a t c g c t a g c g a c g g t c c t a t a t g c a t

600 160 165 170 175 NLAFLGIAYNTLLRIAEIAR Cre NLAFLGIAYNTLLRIAEIAR a t c t g g c a t t t c t g g g g a t t g c t t a t a a c a c c c t g t t a c g t a t a g c c g a a a t t g c c a g g a t a g a c c g t a a a g a c c c c t a a c g a a t a t t g t g g g a c a a t g c a t a t c g g c t t t a a c g g t c c t

EcoRV 660 180 185 190 195 IRVKDISRTDGGRMLIHIGR Cre IRVKDISRTDGGRMLIHIGR t c a g g g t t a a a g a t a t c t c a c g t a c t g a c g g t g g g a g a a t g t t a a t c c a t a t t g g c a g a a a g t c c c a a t t t c t a t a g a g t g c a t g a c t g c c a c c c t c t t a c a a t t a g g t a t a a c c g t c t t

BspMIPaqCIBfuAI 720 200 205 210 215 TKTLVSTAGVEKALSLGVTK Cre TKTLVSTAGVEKALSLGVTK c g a a a a c g c t g g t t a g c a c c g c a g g t g t a g a g a a g g c a c t t a g c c t g g g g g t a a c t a a a c g c t t t t g c g a c c a a t c g t g g c g t c c a c a t c t c t t c c g t g a a t c g g a c c c c c a t t g a t t t g

BsmBIEsp3I 780 220 225 230 235 LVERWISVSGVADDPNNYLF Cre LVERWISVSGVADDPNNYLF t g g t c g a g c g a t g g a t t t c c g t c t c t g g t g t a g c t g a t g a t c c g a a t a a c t a c c t g t t t t a c c a g c t c g c t a c c t a a a g g c a g a g a c c a c a t c g a c t a c t a g g c t t a t t g a t g g a c a a a a

840 240 245 250 255 CRVRKNGVAAPSATSQLSTR Cre CRVRKNGVAAPSATSQLSTR g c c g g g t c a g a a a a a a t g g t g t t g c c g c g c c a t c t g c c a c c a g c c a g c t a t c a a c t c g c g c g g c c c a g t c t t t t t t a c c a c a a c g g c g c g g t a g a c g g t g g t c g g t c g a t a g t t g a g c g c

PshAIBpu10IHaeIIBspDIClaI 900 260 265 270 275 ALEGIFEATHRLIYGAKDDS Cre ALEGIFEATHRLIYGAKDDS c c c t g g a a g g g a t t t t t g a a g c a a c t c a t c g a t t g a t t t a c g g c g c t a a g g a t g a c t c t g g g g a c c t t c c c t a a a a a c t t c g t t g a g t a g c t a a c t a a a t g c c g c g a t t c c t a c t g a g a c

Bme1580IBsp1286IBaeGIMmeI 960 280 285 290 295 GQRYLAWSGHSARVGAARDM Cre GQRYLAWSGHSARVGAARDM g t c a g a g a t a c c t g g c c t g g t c t g g a c a c a g t g c c c g t g t c g g a g c c g c g c g a g a t a t g g c a g t c t c t a t g g a c c g g a c c a g a c c t g t g t c a c g g g c a c a g c c t c g g c g c g c t c t a t a c c

SspIBpmI 1020 300 305 310 315 ARAGVSIPEIMQAGGWTNVN Cre ARAGVSIPEIMQAGGWTNVN c c c g c g c t g g a g t t t c a a t a c c g g a g a t c a t g c a a g c t g g t g g c t g g a c c a a t g t a a a t a g g g c g c g a c c t c a a a g t t a t g g c c t c t a g t a c g t t c g a c c a c c g a c c t g g t t a c a t t t a t

EcoP15IBspHI 1080 320 325 330 335 IVMNYIRNLDSETGAMVRLL Cre IVMNYIRNLDSETGAMVRLL t t g t c a t g a a c t a t a t c c g t a a c c t g g a t a g t g a a a c a g g g g c a a t g g t g c g c c t g c t g g a a c a g t a c t t g a t a t a g g c a t t g g a c c t a t c a c t t t g t c c c c g t t a c c a c g c g g a c g a c c

XbaI 1140 340 EDGD (in frame with Cre) GSLEL* Cre SV40 poly(A) signal EDGDGSLEL* a a g a t g g c g a t g g t t c t c t a g a a c t a t a g t g a g t c g t a t t a c g t a g a t c c a g a c a t g a t a t t c t a c c g c t a c c a a g a g a t c t t g a t a t c a c t c a g c a t a a t g c a t c t a g g t c t g t a c t a t

BtsαIBtsI 1200 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t t a t t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a a t a a

HincIIHpaI 1260 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a g t t a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t c a a

MfeI 1320 a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g g t g t g g g a g g t t t t t t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c c a c a c c c t c c a a a a a

End (1334) EagIEaeINotI 1334 t a a t t c g c g g c c g c a t t a a g c g c c g g c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.