Addgene pmGFPS - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pmGFPS


Map View

M13 Forward (4576 .. 4593) M13/pUC Forward (4562 .. 4584) PluTI (4437) SfoI (4435) NarI (4434) KasI (4433) BstAPI (4383) pRS-marker (4349 .. 4368) pGEX 3' (4227 .. 4249) PfoI (4244) pBRforEco (4171 .. 4189) AatII (4133) ZraI (4131) SspI (4015) XmnI (3810) Amp-R (3761 .. 3780) ScaI (3691) TsoI (3610) NmeAIII (3359) BsrFI (3291) AhdI (3211) pBR322ori-F (2459 .. 2478) L4440 (2208 .. 2225) EcoRI (1) XcmI (3) GFP-R (39 .. 67) BtgI - NcoI (174) BsrGI (284) PmlI (333) HpaI (497) MfeI (568) BtgZI (589) GFP-F (662 .. 682) AvaI - BsoBI - TspMI - XmaI (726) SmaI (728) Bpu10I (760) BseRI (849) BsaBI * (1108) BsmI (1263) BbsI (1337) AvrII (1371) SfiI (1375) BstXI (1443) BsiWI (1466) BclI * (1602) AleI (1613) Acc65I (1672) KpnI (1676) NsiI (1833) BssHII (1862) SalI (1941) BfuAI - BspMI (1954) M13 rev M13 Reverse (1977 .. 1993) lac operator M13/pUC Reverse (1990 .. 2012) BspQI - SapI (2202) pmGFPS 4593 bp

Sequence View

EcoRIXcmI 60 5 10 15 SKGEELFTGVVPILVE GFP (S65T) C A A C A G G G T T A A G A A C A A C T T A GFP-R g a a t t c g t g g c a g t a a a g g a g a a g a a c t t t t c a c t g g a g t t g t c c c a a t t c t t g t t g a a t c t t a a g c a c c g t c a t t t c c t c t t c t t g a a a a g t g a c c t c a a c a g g g t t a a g a a c a a c t t a

120 20 25 30 35 LDGDVNGHKFSVSGEGEGDA GFP (S65T) A T C T A C C GFP-R t a g a t g g t g a t g t t a a t g g g c a c a a a t t t t c t g t c a g t g g a g a g g g t g a a g g t g a t g c a a a t c t a c c a c t a c a a t t a c c c g t g t t t a a a a g a c a g t c a c c t c t c c c a c t t c c a c t a c g t t

NcoIBtgI 180 40 45 50 55 TYGKLTLKFICTTGKLPVPW GFP (S65T) c a t a c g g a a a a c t t a c c c t t a a a t t t a t t t g c a c t a c t g g a a a a c t a c c t g t t c c a t g g c g t a t g c c t t t t g a a t g g g a a t t t a a a t a a a c g t g a t g a c c t t t t g a t g g a c a a g g t a c c g

240 60 65 70 75 PTLVTTFGYGVQCFARYPDH GFP (S65T) c a a c a c t t g t c a c t a c t t t c g g t t a t g g t g t t c a a t g c t t t g c g a g a t a c c c a g a t c a t a g t t g t g a a c a g t g a t g a a a g c c a a t a c c a c a a g t t a c g a a a c g c t c t a t g g g t c t a g t a t

BsrGI 300 80 85 90 95 MKQHDFFKSAMPEGYVQERT GFP (S65T) MKQHDFFKSAMPEGYVQERT t g a a a c a g c a t g a c t t t t t c a a g a g t g c c a t g c c c g a a g g t t a t g t a c a g g a a a g a a c t a a c t t t g t c g t a c t g a a a a a g t t c t c a c g g t a c g g g c t t c c a a t a c a t g t c c t t t c t t g a t

PmlI 360 100 105 110 115 IFFKDDGNYKTRAEVKFEGD GFP (S65T) IFFKDDGNYKTRAEVKFEGD t a t t t t t c a a a g a t g a c g g g a a c t a c a a g a c a c g t g c t g a a g t c a a g t t t g a a g g t g a t a a t a a a a a g t t t c t a c t g c c c t t g a t g t t c t g t g c a c g a c t t c a g t t c a a a c t t c c a c t a t

420 120 125 130 135 TLVNRIELKGIDFKEDGNIL GFP (S65T) TLVNRIELKGIDFKEDGNIL c c c t t g t t a a t a g a a t c g a g t t a a a a g g t a t t g a t t t t a a a g a a g a t g g a a a c a t t c t t g g g g a a c a a t t a t c t t a g c t c a a t t t t c c a t a a c t a a a a t t t c t t c t a c c t t t g t a a g a a c

480 140 145 150 155 GHKLEYNYNSHNVYIMADKQ GFP (S65T) GHKLEYNYNSHNVYIMADKQ g a c a c a a a t t g g a a t a c a a c t a t a a c t c a c a c a a t g t a t a c a t c a t g g c a g a c a a a c a a a c t g t g t t t a a c c t t a t g t t g a t a t t g a g t g t g t t a c a t a t g t a g t a c c g t c t g t t t g t t t

HpaI 540 160 165 170 175 KNGIKVNFKIRHNIEDGSVQ GFP (S65T) KNGIKVNFKIRHNIEDGSVQ a g a a t g g a a t c a a a g t t a a c t t c a a a a t t a g a c a c a a c a t t g a a g a t g g a a g c g t t c a a c t c t t a c c t t a g t t t c a a t t g a a g t t t t a a t c t g t g t t g t a a c t t c t a c c t t c g c a a g t t g

BtgZIMfeI 600 180 185 190 195 LADHYQQNTPIGDGPVLLPD GFP (S65T) LADHYQQNTPIGDGPVLLPD t a g c a g a c c a t t a t c a a c a a a a t a c t c c a a t t g g c g a t g g c c c t g t c c t t t t a c c a g a c a a t c g t c t g g t a a t a g t t g t t t t a t g a g g t t a a c c g c t a c c g g g a c a g g a a a a t g g t c t g t

660 200 205 210 215 NHYLSTQSKLSKDPNEKRDH GFP (S65T) NHYLSTQSKLSKDPNEKRDH a c c a t t a c c t g t c c a c a c a a t c t a a a c t t t c g a a a g a t c c c a a c g a a a a g a g a g a c c a c a t g g t a a t g g a c a g g t g t g t t a g a t t t g a a a g c t t t c t a g g g t t g c t t t t c t c t c t g g t g t

720 220 225 230 235 MVLLEFVTAAGITHGMDELY GFP (S65T) G G T C C T T C T T G A G T T T G T A A C GFP-F MVLLEFVTAAGITHGMDELY t g g t c c t t c t t g a g t t t g t a a c a g c t g c t g g g a t t a c a c a t g g c a t g g a t g a a c t a t a c a a c c a g g a a g a a c t c a a a c a t t g t c g a c g a c c c t a a t g t g t a c c g t a c c t a c t t g a t a t g t

Bpu10ITspMIBsoBIXmaIAvaISmaI 780 K * GFP (S65T) K* a a t a a c c c g g g c c g g g g a t c c g g t g a t t g a t t g a g c a a g c t t a g g a g t c a c t g c c a g g t a t t a t t g g g c c c g g c c c c t a g g c c a c t a a c t a a c t c g t t c g a a t c c t c a g t g a c g g t c c a t

840 t c g t t t g a a c a c g g c a t t a g t c a g g g a a g t c a t a a c a c a g t c c t t t c c c g c a a t t t t c t t a g c a a a c t t g t g c c g t a a t c a g t c c c t t c a g t a t t g t g t c a g g a a a g g g c g t t a a a a g a a

BseRI 900 HIS3 promoter t t t c t a t t a c t c t t g g c c t c c t c t a g t a c a c t c t a t a t t t t t t t a t g c c t c g g t a a t g a t a a a g a t a a t g a g a a c c g g a g g a g a t c a t g t g a g a t a t a a a a a a a t a c g g a g c c a t t a c t a

960 HIS3 promoter t t t c a t t t t t t t t t t t c c a c c t a g c g g a t g a c t c t t t t t t t t t c t t a g c g a t t g g c a t t a a a a g t a a a a a a a a a a a g g t g g a t c g c c t a c t g a g a a a a a a a a a g a a t c g c t a a c c g t a a t

1020 HIS3 promoter t c a c a t a a t g a a t t a t a c a t t a t a t a a a g t a a t g t g a t t t c t t c g a a g a a t a t a c t a a a a a g t g t a t t a c t t a a t a t g t a a t a t a t t t c a t t a c a c t a a a g a a g c t t c t t a t a t g a t t t t

1080 1 5 10 MTEQKALVKR HIS3 promoter HIS3 MTEQKALVKR a a t g a g c a g g c a a g a t a a a c g a a g g c a a a g a t g a c a g a g c a g a a a g c c c t a g t a a a g c g t t t a c t c g t c c g t t c t a t t t g c t t c c g t t t c t a c t g t c t c g t c t t t c g g g a t c a t t t c g c a

BsaBI* 1140 15 20 25 30 ITNETKIQIAISLKGGPLAI HIS3 ITNETKIQIAISLKGGPLAI a t t a c a a a t g a a a c c a a g a t t c a g a t t g c g a t c t c t t t a a a g g g t g g t c c c c t a g c g a t a t a a t g t t t a c t t t g g t t c t a a g t c t a a c g c t a g a g a a a t t t c c c a c c a g g g g a t c g c t a t

1200 35 40 45 50 EHSIFPEKEAEAVAEQATQS HIS3 EHSIFPEKEAEAVAEQATQS g a g c a c t c g a t c t t c c c a g a a a a a g a g g c a g a a g c a g t a g c a g a a c a g g c c a c a c a a t c g c t c g t g a g c t a g a a g g g t c t t t t t c t c c g t c t t c g t c a t c g t c t t g t c c g g t g t g t t a g c

1260 55 60 65 70 QVINVHTGIGFLDHMIHALA HIS3 QVINVHTGIGFLDHMIHALA c a a g t g a t t a a c g t c c a c a c a g g t a t a g g g t t t c t g g a c c a t a t g a t a c a t g c t c t g g c c g t t c a c t a a t t g c a g g t g t g t c c a t a t c c c a a a g a c c t g g t a t a c t a t g t a c g a g a c c g g

BsmI 1320 75 80 85 90 KHSGWSLIVECIGDLHIDDH HIS3 KHSGWSLIVECIGDLHIDDH a a g c a t t c c g g c t g g t c g c t a a t c g t t g a g t g c a t t g g t g a c t t a c a c a t a g a c g a c c a t t t c g t a a g g c c g a c c a g c g a t t a g c a a c t c a c g t a a c c a c t g a a t g t g t a t c t g c t g g t a

AvrIISfiIBbsI 1380 95 100 105 110 HTTEDCGIALGQAFKEALGA HIS3 HTTEDCGIALGQAFKEALGA c a c a c c a c t g a a g a c t g c g g g a t t g c t c t c g g t c a a g c t t t t a a a g a g g c c c t a g g g g c c g t g t g g t g a c t t c t g a c g c c c t a a c g a g a g c c a g t t c g a a a a t t t c t c c g g g a t c c c c g g

1440 115 120 125 130 VRGVKRFGSGFAPLDEALSR HIS3 VRGVKRFGSGFAPLDEALSR g t g c g t g g a g t a a a a a g g t t t g g a t c a g g a t t t g c g c c t t t g g a t g a g g c a c t t t c c a g a c a c g c a c c t c a t t t t t c c a a a c c t a g t c c t a a a c g c g g a a a c c t a c t c c g t g a a a g g t c t

BsiWIBstXI 1500 135 140 145 150 AVVDLSNRPYAVVELGLQRE HIS3 AVVDLSNRPYAVVELGLQRE g c g g t g g t a g a t c t t t c g a a c a g g c c g t a c g c a g t t g t c g a a c t t g g t t t g c a a a g g g a g c g c c a c c a t c t a g a a a g c t t g t c c g g c a t g c g t c a a c a g c t t g a a c c a a a c g t t t c c c t c

1560 155 160 165 170 KVGDLSCEMIPHFLESFAEA HIS3 KVGDLSCEMIPHFLESFAEA a a a g t a g g a g a t c t c t c t t g c g a g a t g a t c c c g c a t t t t c t t g a a a g c t t t g c a g a g g c t t t t c a t c c t c t a g a g a g a a c g c t c t a c t a g g g c g t a a a a g a a c t t t c g a a a c g t c t c c g a

AleIBclI* 1620 175 180 185 190 SRITLHVDCLRGKNDHHRSE HIS3 SRITLHVDCLRGKNDHHRSE a g c a g a a t t a c c c t c c a c g t t g a t t g t c t g c g a g g c a a g a a t g a t c a t c a c c g t a g t g a g t c g t c t t a a t g g g a g g t g c a a c t a a c a g a c g c t c c g t t c t t a c t a g t a g t g g c a t c a c t c

Acc65IKpnI 1680 195 200 205 210 SAFKALAVAIREATSPNGTN HIS3 SAFKALAVAIREATSPNGTN a g t g c g t t c a a g g c t c t t g c g g t t g c c a t a a g a g a a g c c a c c t c g c c c a a t g g t a c c a a c t c a c g c a a g t t c c g a g a a c g c c a a c g g t a t t c t c t t c g g t g g a g c g g g t t a c c a t g g t t g

1740 215 220 DVPSTKGVLM* HIS3 DVPSTKGVLM* g a t g t t c c c t c c a c c a a a g g t g t t c t t a t g t a g t g a c a c c g a t t a t t t a a a g c t g c a g c a c t a c a a g g g a g g t g g t t t c c a c a a g a a t a c a t c a c t g t g g c t a a t a a a t t t c g a c g t c g t

1800 t a c g a t a t a t a t a c a t g t g t a t a t a t g t a t a c c t a t g a a t g t c a g t a a g t a t g t a t a c g a a t g c t a t a t a t a t g t a c a c a t a t a t a c a t a t g g a t a c t t a c a g t c a t t c a t a c a t a t g c t

NsiI 1860 a c a g t a t g a t a c t g a a g a t g a c a a g g t a a t g c a t c a t t c t a t a c g t g t c a t t c t g a a c g a t g t c a t a c t a t g a c t t c t a c t g t t c c a t t a c g t a g t a a g a t a t g c a c a g t a a g a c t t g c t

BssHII 1920 g g c g c g c t t t c c t t t t t t c t t t t t g c t t t t t c t t t t t t t t t c t c t t g a a c t c g a a g c t t g c c g c g c g a a a g g a a a a a a g a a a a a c g a a a a a g a a a a a a a a a g a g a a c t t g a g c t t c g a a c

BspMIBfuAISalI 1980 M13 rev C A G T M13 Reverse c t c a a t c a a t c a c c g g a t c c g t c g a c c t g c a g c c a a g c t a g c t t g g c g t a a t c a t g g t c a g a g t t a g t t a g t g g c c t a g g c a g c t g g a c g t c g g t t c g a t c g a a c c g c a t t a g t a c c a g t

2040 -10 M13 rev lac operator lac promoter A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t

2100 -35 lac promoter CAP binding site a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c

2160 c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g

BspQISapI 2220 G A G C G A G T G A C T G L4440 c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g

2280 A G C G A L4440 t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t

2340 c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t

2400 ori a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a

2460 ori T T pBR322ori-F g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t

2520 ori T C T A T G G T C C G C A A A G G G pBR322ori-F a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c

2580 ori c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t

2640 ori c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t

2700 ori c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c

2760 ori g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c

2820 ori t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g g a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c c

2880 ori a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g

2940 ori t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c

3000 ori a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g

3060 g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g

3120 t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c

3180 285 280 275 *WHKILSAGIEAIQ AmpR *WHKILSAGIEAIQ t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a

AhdI 3240 270 265 260 255 RNREDMTAQSGTTYIVVIRS AmpR RNREDMTAQSGTTYIVVIRS c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c

BsrFI 3300 250 245 240 235 PKGDPGLAAIIGRSGREGAG AmpR PKGDPGLAAIIGRSGREGAG g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t

NmeAIII 3360 230 225 220 215 SKDAIFWGAPLASRLLPGAV AmpR SKDAIFWGAPLASRLLPGAV g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a

3420 210 205 200 195 KDAEMWDILQQRSALTLLEG AmpR KDAEMWDILQQRSALTLLEG t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t

3480 190 185 180 175 TLLKRLTTAMAVPMTTDRED AmpR TLLKRLTTAMAVPMTTDRED g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c

3540 170 165 160 155 NPIAENLEPEWRDLRTVHDG AmpR NPIAENLEPEWRDLRTVHDG t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g

3600 150 145 140 135 MNHLFATLEKPGGITTLLLN AmpR MNHLFATLEKPGGITTLLLN a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c

TsoI 3660 130 125 120 115 AATNDSMTIAASCLERVTMG AmpR AATNDSMTIAASCLERVTMG g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t

ScaI 3720 110 105 100 95 DTLHKETVPSYEVLDNQSYH AmpR DTLHKETVPSYEVLDNQSYH t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a

3780 90 85 80 75 IRRGLQEQGADIRSLVAGCL AmpR A T A A T A C C G C G C C A C A T A G C Amp-R IRRGLQEQGADIRSLVAGCL a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g

XmnI 3840 70 65 60 55 LVKFTSMMPFREEPRFSELI AmpR LVKFTSMMPFREEPRFSELI a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g

3900 50 45 40 35 KGSNLDLEIYGVRAGLQDEA AmpR KGSNLDLEIYGVRAGLQDEA t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t

3960 30 25 DKVKVLTEPH 20 15 signal sequence AFVPLCFAAF AmpR DKVKVLTEPHAFVPLCFAAF t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t

SspI 4020 10 5 1 signal sequence FPILAVRFHQISM AmpR AmpR promoter FPILAVRFHQISM a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a

4080 AmpR promoter t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t

ZraIAatII 4140 AmpR promoter a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t a a g a a t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g a t t c t t

4200 A A T A G G C G T A T C A C G A G G C pBRforEco a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c c c t t t c g t c t c t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g g g a a a g c a g a g

PfoI 4260 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' g c g c g t t t c g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a c a c g c g c a a a g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t g t

4320 g c t t g t c t g t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g t t c g a a c a g a c a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c a a

4380 C G G C A T C A G A G C A G A T T G T A pRS-marker g g c g g g t g t c g g g g c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a c c c g c c c a c a g c c c c g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g t g

KasINarISfoIPluTIBstAPI 4440 c a t a t g c g g t g t g a a a t a c c g c a c a g a t g c g t a a g g a g a a a a t a c c g c a t c a g g c g c c a t g t a t a c g c c a c a c t t t a t g g c g t g t c t a c g c a t t c c t c t t t t a t g g c g t a g t c c g c g g t a

4500 t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a a t

4560 c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c c a g g g t t t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g g t c c c a a a

4593 M13 fwd C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.