Addgene pcDNA3-N-Flag-NLRP3 91-710 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pcDNA3-N-Flag-NLRP3 91-710


Map View

SgrDI (7309) SspI (7192) Amp-R (6938 .. 6957) ScaI (6868) PvuI (6758) AhdI (6388) pBR322ori-F (5636 .. 5655) L4440 (5385 .. 5402) CAP binding site M13/pUC Reverse (5167 .. 5189) lac operator M13 Reverse (5154 .. 5170) BstZ17I (5116) EBV-rev (5075 .. 5094) BsmI (5064) SV40pA-R (5021 .. 5040) BstBI (4826) Neo-F (4680 .. 4699) RsrII (4660) BssHII (4541) PflFI - Tth111I (4262) MscI (4226) PluTI (4147) SfoI (4145) NarI (4144) KasI (4143) Neo-R (4070 .. 4089) BsaBI * (4003) SV40pro-F (3862 .. 3881) pBABE 3' (3614 .. 3634) pRS-marker (44 .. 63) MfeI (161) Bpu10I (180) NruI (208) MluI (228) SpeI (249) CMV enhancer NdeI (484) SnaBI (590) CMV-F (769 .. 789) T7 (863 .. 882) HindIII (889) Acc65I (895) KpnI (899) BspDI - ClaI (947) EcoRI (955) AflII (1287) BamHI (1567) BlpI (1674) SfiI (1860) EcoNI (2130) PshAI (2172) BmgBI (2357) BstXI (2567) PasI (2726) PaeR7I - PspXI - XhoI (2836) XbaI (2848) PspOMI (2854) ApaI (2858) SP6 (2864 .. 2881) BGH-rev (2901 .. 2918) F1ori-R (3264 .. 3283) F1ori-F (3474 .. 3495) pcDNA3-N-Flag-NLRP3 91-710 7311 bp

Sequence View

5ʹ 3ʹ 60 A T G T T A G A C G A G A C T A C pRS-marker g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t c g a c t c t c a g t a c a a t c t g c t c t g a t g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a g c t g a g a g t c a t g t t a g a c g a g a c t a c

120 G G C pRS-marker c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c

MfeI 180 c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g

MluINruIBpu10I 240 CMV enhancer t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a

SpeI 300 CMV enhancer g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t

360 CMV enhancer t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g

420 CMV enhancer c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g

480 CMV enhancer a t t g a c g t c a a t g g g t g g a c t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t t a a c t g c a g t t a c c c a c c t g a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a

NdeI 540 CMV enhancer a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a

SnaBI 600 CMV enhancer a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t

660 CMV enhancer CMV promoter t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c

720 CMV promoter a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g

780 CMV promoter C G C A A A T G G G C G CMV-F a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c

840 CMV promoter G T A G G C G T G CMV-F g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c t c t g g c t a a c t a g a g a a c c c a c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g a g a c c g a t t g a t c t c t t g g g t

HindIIIAcc65IKpnI 900 T7 promoter T A A T A C G A C T C A C T A T A G G G T7 c t g c t t a c t g g c t t a t c g a a a t t a a t a c g a c t c a c t a t a g g g a g a c c c a a g c t t g g t a c c g a c g a a t g a c c g a a t a g c t t t a a t t a t g c t g a g t g a t a t c c c t c t g g g t t c g a a c c a t g g

EcoRIBspDIClaI 960 1 5 DYKDDDDK (in frame with FLAG) IDIEF FLAG g a g c t c g g a t c g c c c a c c a t g g a c t a c a a a g a c g a t g a c g a t a a a a t c g a t a t c g a a t t c c t c g a g c c t a g c g g g t g g t a c c t g a t g t t t c t g c t a c t g c t a t t t t a g c t a t a g c t t a a g

1020 (in frame with FLAG) MSGMTRVHHIPLWYARRTAL MVCQEDSL a t g a g t g g a a t g a c a c g t g t a c a t c a c a t t c c t c t a t g g t a t g c c a g g a g g a c a g c c t t g t a c t c a c c t t a c t g t g c a c a t g t a g t g t a a g g a g a t a c c a t a c g g t c c t c c t g t c g g a a c

1080 (in frame with FLAG) KKSGWVCWDISPASPFVKRR EEEWMGLLGYLSRISICKKK a a g a a g a g t g g a t g g g t t t g c t g g g a t a t c t c t c c c g c a t c t c c a t t t g t a a a a a g a a g a t t c t t c t c a c c t a c c c a a a c g a c c c t a t a g a g a g g g c g t a g a g g t a a a c a t t t t t c t t c t

1140 (in frame with FLAG) KITVRCTDDM* KDYCKMYRRHVRSRFYSIKD a a g a t t a c t g t a a g a t g t a c a g a c g a c a t g t g a g a a g c a g g t t c t a c t c t a t c a a g g a c a t t c t a a t g a c a t t c t a c a t g t c t g c t g t a c a c t c t t c g t c c a a g a t g a g a t a g t t c c t g t

1200 RNARLGESVDLNSRYTQLQL g g a a c g c g c g t c t a g g t g a g a g t g t g g a c c t c a a c a g t c g c t a c a c g c a g c t c c a a c t g g c c t t g c g c g c a g a t c c a c t c t c a c a c c t g g a g t t g t c a g c g a t g t g c g t c g a g g t t g a c c

1260 VKEHPSKQEREHELLTIGRT t c a a g g a g c a t c c a a g c a a g c a g g a g c g g g a g c a t g a a c t c c t g a c c a t c g g c c g g a c t a a g t t c c t c g t a g g t t c g t t c g t c c t c g c c c t c g t a c t t g a g g a c t g g t a g c c g g c c t g a t

AflII 1320 KMRDSPMSSLKLELLFEPED a a a t g c g g g a c a g c c c c a t g a g t t c c c t t a a g c t g g a g c t g c t g t t t g a g c c c g a g g a c g t t t a c g c c c t g t c g g g g t a c t c a a g g g a a t t c g a c c t c g a c g a c a a a c t c g g g c t c c t g c

1380 GHSEPVHTVVFQGAAGIGKT g g c a c t c g g a g c c t g t g c a c a c a g t g g t g t t c c a g g g a g c a g c a g g c a t c g g g a a a a c c a c c g t g a g c c t c g g a c a c g t g t g t c a c c a c a a g g t c c c t c g t c g t c c g t a g c c c t t t t g g t

1440 ILARKIMLDWALGKLFKDKF t c c t a g c c a g g a a g a t t a t g t t g g a c t g g g c a c t g g g a a a g c t c t t c a a a g a c a a a t t t g a g g a t c g g t c c t t c t a a t a c a a c c t g a c c c g t g a c c c t t t c g a g a a g t t t c t g t t t a a a c

1500 DYLFFIHCREVSLRTPRSLA *KNKIWQRSTLRLVGLLRAS a c t a t t t g t t c t t t a t c c a c t g c c g a g a g g t g a g c c t c a g g a c g c c a a g g a g t c t a g c a g t g a t a a a c a a g a a a t a g g t g a c g g c t c t c c a c t c g g a g t c c t g c g g t t c c t c a g a t c g t c

1560 DLIVSCWPDPNPPVCKILRK RITLQQGSGFGGTHLIRRLG a c c t g a t t g t c a g c t g c t g g c c t g a c c c a a a c c c a c c a g t g t g c a a g a t c c t g c g c a a g c t g g a c t a a c a g t c g a c g a c c g g a c t g g g t t t g g g t g g t c a c a c g t t c t a g g a c g c g t t c g

BamHI 1620 PSRILFLMDGFDELQGAFDE ELIRKRMSPKSSSCPAKSSC c t t c c a g g a t c c t c t t c c t c a t g g a t g g c t t t g a t g a g c t a c a a g g g g c c t t t g a c g a g c g a a g g t c c t a g g a g a a g g a g t a c c t a c c g a a a c t a c t c g a t g t t c c c c g g a a a c t g c t c g

BlpI 1680 HIGEVCTDWQKAVRGDILLS MPSTQVSQCFATRPSMRSLL a c a t t g g g g a g g t c t g c a c a g a c t g g c a a a a g g c t g t g c g g g g a g a c a t t c t g c t a a g c a t g t a a c c c c t c c a g a c g t g t c t g a c c g t t t t c c g a c a c g c c c c t c t g t a a g a c g a t t c g t

1740 SLIRKKLLPKASLLITTRPV RMRFFSSGLAERSMVVLGTA g c c t c a t c c g a a a g a a a c t g c t g c c c a a g g c c t c t c t g c t c a t a a c g a c g a g g c c g g t a g c g g a g t a g g c t t t c t t t g a c g a c g g g t t c c g g a g a g a c g a g t a t t g c t g c t c c g g c c a t c

1800 ALEKLQHLLDHPRHVEILGF KSFSCCRRSWGRWTSIRPKE c c t t g g a g a a a c t g c a g c a t c t c c t g g a c c a c c c c c g c c a t g t g g a g a t c c t a g g t t t c t g g a a c c t c t t t g a c g t c g t a g a g g a c c t g g t g g g g g c g g t a c a c c t c t a g g a t c c a a a g a

SfiI 1860 SEAKRKEYFFKYFSNELQAR SALLFSYKKLYKELSSCARS c t g a g g c c a a a a g g a a g g a g t a t t t c t t t a a g t a t t t c t c c a a c g a g c t g c a g g c c c g g g g a c t c c g g t t t t c c t t c c t c a t a a a g a a a t t c a t a a a g a g g t t g c t c g a c g t c c g g g c c c

1920 EAFRLIQENEVLFTMCFIPL AKLSIWSFSTRKVMHKM a g g c c t t c a g g c t g a t c c a a g a g a a t g a g g t c c t c t t t a c c a t g t g c t t c a t c c c c c t g g t c c g g a a g t c c g a c t a g g t t c t c t t a c t c c a g g a g a a a t g g t a c a c g a a g t a g g g g g a c c

1980 VCWIVCTGLKQQMETGKSLA t c t g c t g g a t t g t g t g c a c g g g g c t a a a g c a a c a g a t g g a g a c c g g g a a g a g c c t g g c c c a g a c g a c c t a a c a c a c g t g c c c c g a t t t c g t t g t c t a c c t c t g g c c c t t c t c g g a c c g g g

2040 QTSKTTTAVYVFFLSSLLQS a g a c c t c c a a g a c c a c t a c g g c c g t c t a c g t c t t c t t c c t t t c c a g c c t g c t g c a a t c c c t c t g g a g g t t c t g g t g a t g c c g g c a g a t g c a g a a g a a g g a a a g g t c g g a c g a c g t t a g g g

2100 RGGIEEHLFSDYLQGLCSLA g g g g g g g c a t t g a g g a g c a t c t c t t c t c t g a c t a c c t a c a g g g g c t c t g t t c a c t g g c t g c c c c c c c g t a a c t c c t c g t a g a g a a g a g a c t g a t g g a t g t c c c c g a g a c a a g t g a c c g a c

EcoNI 2160 ADGIWNQKILFEECDLRKHG c g g a t g g a a t t t g g a a c c a g a a a a t c c t a t t t g a g g a g t g t g a t c t g c g g a a g c a c g g c c g c c t a c c t t a a a c c t t g g t c t t t t a g g a t a a a c t c c t c a c a c t a g a c g c c t t c g t g c c g g

PshAI 2220 LQKTDVSAFLRMNVFQKEVD t g c a g a a g a c t g a c g t c t c c g c t t t c c t g a g g a t g a a c g t g t t c c a g a a g g a a g t g g a c t a c g t c t t c t g a c t g c a g a g g c g a a a g g a c t c c t a c t t g c a c a a g g t c t t c c t t c a c c t g a

2280 CERFYSFSHMTFQEFFAAMY g c g a g a g a t t c t a c a g c t t c a g c c a c a t g a c t t t c c a g g a g t t c t t c g c t g c t a t g t a c t c g c t c t c t a a g a t g t c g a a g t c g g t g t a c t g a a a g g t c c t c a a g a a g c g a c g a t a c a t g a

2340 YLLEEEAEGETVRKGPGGCS a t t t g c t g g a a g a g g a g g c a g a g g g g g a g a c c g t g a g g a a a g g a c c a g g a g g t t g t t c a g t a a a c g a c c t t c t c c t c c g t c t c c c c c t c t g g c a c t c c t t t c c t g g t c c t c c a a c a a g t c

BmgBI 2400 DLLNRDVKVLLENYGKFEKG a t c t t c t g a a c c g a g a c g t g a a g g t c c t a c t a g a a a a t t a c g g c a a g t t t g a a a a a g g c t t a g a a g a c t t g g c t c t g c a c t t c c a g g a t g a t c t t t t a a t g c c g t t c a a a c t t t t t c c g a

2460 YLIFVVRFLFGLVNQERTSY a t c t g a t t t t t g t t g t c c g a t t c c t c t t t g g c c t t g t a a a c c a g g a g a g a a c c t c t t a t t t a g a c t a a a a a c a a c a g g c t a a g g a g a a a c c g g a a c a t t t g g t c c t c t c t t g g a g a a t a a

2520 LEKKLSCKISQQVRLELLKW t g g a g a a g a a a c t a a g t t g c a a g a t c t c t c a g c a a g t c a g a c t g g a a c t a c t g a a g t g g a a c c t c t t c t t t g a t t c a a c g t t c t a g a g a g t c g t t c a g t c t g a c c t t g a t g a c t t c a c c t

BstXI 2580 IEVKAKAKKLQWQPSQLELF t t g a a g t g a a a g c c a a g g c c a a g a a g c t g c a g t g g c a g c c c a g c c a a c t g g a a c t g t t c t a a c t t c a c t t t c g g t t c c g g t t c t t c g a c g t c a c c g t c g g g t c g g t t g a c c t t g a c a a g a

2640 YCLYEMQEEDFVQSAMDHFP a c t g c c t g t a c g a g a t g c a g g a g g a a g a c t t t g t g c a g a g t g c c a t g g a c c a c t t t c c c a t g a c g g a c a t g c t c t a c g t c c t c c t t c t g a a a c a c g t c t c a c g g t a c c t g g t g a a a g g g t

2700 KIEINLSTRMDHVVSSFCIK a a a t t g a g a t c a a c c t c t c t a c c a g a a t g g a c c a c g t g g t t t c c t c c t t t t g t a t t a a g a t t t a a c t c t a g t t g g a g a g a t g g t c t t a c c t g g t g c a c c a a a g g a g g a a a a c a t a a t t c t

PasI 2760 NCHRVKTLSLGFFHNSPKEE a c t g t c a t a g g g t c a a a a c g c t t t c c c t g g g t t t t t t t c a c a a c t c g c c c a a g g a g g a a g t g a c a g t a t c c c a g t t t t g c g a a a g g g a c c c a a a a a a a g t g t t g a g c g g g t t c c t c c t t c

2820 EEERRGGRPLDQVQCVFPDT a a g a a g a g a g g a g a g g a g g t c g a c c c t t g g a c c a g g t t c a g t g t g t t t t c c c a g a c a c t c t t c t t c t c t c c t c t c c t c c a g c t g g g a a c c t g g t c c a a g t c a c a c a a a a g g g t c t g t g a g

XbaIPspOMIApaIPaeR7IPspXIXhoI 2880 SP6 promoter G A T A T C A C A G T G G A T T T SP6 HVACLARACI* a t g t t g c c t g t t t a g c t c g a g c a t g c a t c t a g a g g g c c c t a t t c t a t a g t g t c a c c t a a a t a c a a c g g a c a a a t c g a g c t c g t a c g t a g a t c t c c c g g g a t a a g a t a t c a c a g t g g a t t t

2940 SP6 promoter bGH poly(A) signal A SP6 G G A G C T G A C A C G G A A G A T BGH-rev t g c t a g a g c t c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g a c g a t c t c g a g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c

3000 bGH poly(A) signal c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t

3060 bGH poly(A) signal a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a

3120 bGH poly(A) signal g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a t g c t g g g g a t g c g g t c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t a c g a c c c c t a c g c c a

3180 bGH poly(A) signal f1 ori g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t g g g g c t c t a g g g g g t a t c c c c a c g c c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g a c c c c g a g a t c c c c c a t a g g g g t g c g

3240 f1 ori g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g

3300 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a

3360 f1 ori c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g c a t c c c t t t a g g g t t c c g a t t t a g t g c g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c g t a g g g a a a t c c c a a g g c t a a a t c a c g

3420 f1 ori t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g

3480 f1 ori G T G G A C T F1ori-F g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a

3540 f1 ori C T T G T T C C A A A C T G G F1ori-F c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c

3600 f1 ori g a t t t t g g g g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c c t a a a a c c c c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g

3660 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g g c c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c c g

3720 SV40 promoter a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g

3780 SV40 promoter a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a

3840 SV40 promoter SV40 ori c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g

3900 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c t g c c t c t g a g c t g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g a c g g a g a c t c g a

3960 SV40 promoter SV40 ori a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t c c c g g g t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a g g g c c c

BsaBI* 4020 1 MI NeoR/KanR MI a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a a g a g a c a g g a t g a g g a t c g t t t c g c a t g a t t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t t c t c t g t c c t a c t c c t a g c a a a g c g t a c t a

4080 5 10 15 20 EQDGLHAGSPAAWVERLFGY NeoR/KanR G A T A A G C C G A T Neo-R EQDGLHAGSPAAWVERLFGY t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g g c t a t t c g g c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t c c g a t a a g c c g a t

4140 25 30 35 40 DWAQQTIGCSDAAVFRLSAQ NeoR/KanR A C T G A C C C G Neo-R DWAQQTIGCSDAAVFRLSAQ t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c g g c t g t c a g c g c a a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g c c g a c a g t c g c g t

KasINarISfoIPluTI 4200 45 50 55 60 GRPVLFVKTDLSGALNELQD NeoR/KanR GRPVLFVKTDLSGALNELQD g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a a t g a a c t g c a g g a c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t t a c t t g a c g t c c t

MscI 4260 65 70 75 80 EAARLSWLATTGVPCAAVLD NeoR/KanR EAARLSWLATTGVPCAAVLD c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g c a g c t g t g c t c g a g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c g t c g a c a c g a g c t

Tth111IPflFI 4320 85 90 95 100 VVTEAGRDWLLLGEVPGQDL NeoR/KanR VVTEAGRDWLLLGEVPGQDL c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c c g g g g c a g g a t c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g g c c c c g t c c t a g a

4380 105 110 115 120 LSSHLAPAEKVSIMADAMRR NeoR/KanR LSSHLAPAEKVSIMADAMRR *RVKSRGLFYGDHSICHPP c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g a t g c a a t g c g g c g g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c t a c g t t a c g c c g c

4440 125 130 135 140 LHTLDPATCPFDHQAKHRIE NeoR/KanR LHTLDPATCPFDHQAKHRIE QMRKIRSGAWEVVLRFMADL g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a a a c a t c g c a t c g a c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t t t g t a g c g t a g c t

4500 145 150 155 160 RARTRMEAGLVDQDDLDEEH NeoR/KanR RARTRMEAGLVDQDDLDEEH SCTSPHFGTKDILIIQVFLM g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t g g a c g a a g a g c a c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g a c c t g c t t c t c g t

BssHII 4560 165 170 175 180 QGLAPAELFARLKARMPDGE NeoR/KanR QGLAPAELFARLKARMPDGE LPERWGFQEGPELRAHGVAL t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g c g c a t g c c c g a c g g c g a a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c g c g t a c g g g c t g c c g c t

4620 185 190 195 200 DLVVTHGDACLPNIMVENGR NeoR/KanR DLVVTHGDACLPNIMVENGR IEDHGMAIGAQRIDHHFIAA g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g t g g a a a a t g g c c g c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c a c c t t t t a c c g g c

RsrII 4680 205 210 215 220 FSGFIDCGRLGVADRYQDIA NeoR/KanR C Neo-F FSGFIDCGRLGVADRYQDIA KRSEDVTAPQTHRVAILVYR c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a t c a g g a c a t a g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a t a g t c c t g t a t c g

4740 225 230 235 240 LATRDIAEELGGEWADRFLV NeoR/KanR G T T G G C T A C C C G T G A T A T T Neo-F LATRDIAEELGGEWADRFLV QSGTINSFLKAAFPSVAEEH g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g a c c g c t t c c t c g t c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c t g g c g a a g g a g c a

4800 245 250 255 260 LYGIAAPDSQRIAFYRLLDE NeoR/KanR LYGIAAPDSQRIAFYRLLDE KVTDGSGIRLADGEIAKKVL g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c g c c t t c t t g a c g a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g c g g a a g a a c t g c t

BstBI 4860 265 FF* NeoR/KanR FF* EESRSEPTRFSRGLSAWGAM g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c g c c c a a c c t g c c a c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g c g g g t t g g a c g g t

4920 t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g

4980 c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g

5040 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g

BsmI 5100 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t

BstZ17I 5160 SV40 poly(A) signal M13 rev C A G T A T C M13 Reverse t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t a a t c a t g g t c a t a g a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c a t t a g t a c c a g t a t c

5220 -10 M13 rev lac operator lac promoter G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g

5280 -35 lac promoter CAP binding site a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g

5340 t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t

5400 G A G C G A G T G A C T G A G C L4440 c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c

5460 G A L4440 c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c

5520 t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c

5580 ori g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g

5640 ori T T T C T pBR322ori-F g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t

5700 ori A T G G T C C G C A A A G G G pBR322ori-F t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a

5760 ori a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a a t g c t c a c g c t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t t a c g a g t g c g

5820 ori t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g

5880 ori c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t

5940 ori a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a

6000 ori g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g g a c a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c c t g t

6060 ori g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a

6120 ori t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a

6180 ori a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a

6240 c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g

6300 a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t

6360 285 280 275 *WHKILSAGIEAIQR AmpR *WHKILSAGIEAIQR a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t

AhdI 6420 270 265 260 255 NREDMTAQSGTTYIVVIRSP AmpR NREDMTAQSGTTYIVVIRSP t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g

6480 250 245 240 235 KGDPGLAAIIGRSGREGAGS AmpR KGDPGLAAIIGRSGREGAGS t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a

6540 230 225 220 215 KDAIFWGAPLASRLLPGAVK AmpR KDAIFWGAPLASRLLPGAVK t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t

6600 210 205 200 195 DAEMWDILQQRSALTLLEGT AmpR DAEMWDILQQRSALTLLEGT t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a

6660 190 185 180 175 LLKRLTTAMAVPMTTDREDN AmpR LLKRLTTAMAVPMTTDREDN a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a

6720 170 165 160 155 PIAENLEPEWRDLRTVHDGM AmpR PIAENLEPEWRDLRTVHDGM g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c

PvuI 6780 150 145 140 135 NHLFATLEKPGGITTLLLNA AmpR NHLFATLEKPGGITTLLLNA t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g

6840 130 125 120 115 ATNDSMTIAASCLERVTMGD AmpR ATNDSMTIAASCLERVTMGD g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g

ScaI 6900 110 105 100 95 TLHKETVPSYEVLDNQSYHI AmpR TLHKETVPSYEVLDNQSYHI g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c

6960 90 85 80 75 RRGLQEQGADIRSLVAGCLL AmpR A T A A T A C C G C G C C A C A T A G C Amp-R RRGLQEQGADIRSLVAGCLL c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t

7020 70 65 60 55 VKFTSMMPFREEPRFSELIK AmpR VKFTSMMPFREEPRFSELIK a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t

7080 50 45 40 35 GSNLDLEIYGVRAGLQDEAD AmpR GSNLDLEIYGVRAGLQDEAD c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a

7140 30 25 KVKVLTEPH 20 15 signal sequence AFVPLCFAAFF AmpR KVKVLTEPHAFVPLCFAAFF t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c

SspI 7200 10 5 1 signal sequence PILAVRFHQISM AmpR AmpR promoter PILAVRFHQISM g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t

7260 AmpR promoter a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a

SgrDI 3ʹ 5ʹ 7311 AmpR promoter a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.