Addgene mEos4a-CytERM-N-17 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: mEos4a-CytERM-N-17


Map View

1000 750 500 250 End (1065) HpaI (1050) MfeI (1037) DraI (989) BsaBI * (949) XbaI * (941) NotI - EaeI - EagI (931) NspI - SphI (888) PflMI (821) Bpu10I (784) AcuI - Eco57MI (749) NdeI (721) Bsu36I (499) BtsI - BtsαI (421) EcoRV (413) EciI (403) AleI (246) AgeI - BsrFI (233) SmaI (225) ApaI (224) AvaI - XmaI - BsoBI - TspMI (223) PspOMI (220) SacII (219) MspA1I (218) AccI (207) SalI (206) PstI (205) SfcI (201) ApoI - EcoRI (196) PspFI (129) BseYI (125) XcmI (113) EarI (88) BglII (59) HaeII (48) AfeI (46) BmtI (45) NheI (41) Start (0) mEos4a Kozak sequence mEos4a-CytERM-N-17 1065 bp

Sequence View

BglIINheIBmtIAfeIHaeIIStart (0) 5ʹ 3ʹ 60 LYKQSWFSEPSDPLALPDS g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a c c g g a c t c a g c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t g g c c t g a g t c

XcmIEarI 120 DLVPLAASSCQEEKAPMDPV a t c t g g t a c c c c t t g c a g c t t c c a g t t g t c a g g a a g a g a a g g c t c c c a t g g a t c c t g t g g t a g a c c a t g g g g a a c g t c g a a g g t c a a c a g t c c t t c t c t t c c g a g g g t a c c t a g g a c a c c

BseYIPspFI 180 VVLGLCLSCLLLLSLWKQSY *EPFLAI t g g t g c t g g g g c t c t g t c t c t c c t g t t t g c t t c t c c t t t c a c t c t g g a a a c a g a g c t a t g a c c a c g a c c c c g a g a c a g a g a g g a c a a a c g a a g a g g a a a g t g a g a c c t t t g t c t c g a t a c

BsrFIAgeIMspA1ISacIIPspOMITspMIBsoBIXmaIAvaIApaISmaIEcoRIApoISfcIPstISalIAccI 240 Kozak sequence GGGKLRILQSTVPRARDPPV PSPLKSNQLRRYRPGPIWRD g g g g a g g g a a g c t t c g a a t t c t g c a g t c g a c g g t a c c g c g g g c c c g g g a t c c a c c g g t c g c c c c t c c c t t c g a a g c t t a a g a c g t c a g c t g c c a t g g c g c c c g g g c c c t a g g t g g c c a g c

AleI 300 1 5 10 15 MVSAIKPDMRIKLRMEGN mEos4a Kozak sequence ATMVSAIKPDMRIKLRMEGN GGHHTRNLWVHPDFETHFAV c c a c c a t g g t g a g t g c g a t t a a g c c a g a c a t g a g g a t c a a a c t c c g t a t g g a a g g c a a c g g g t g g t a c c a c t c a c g c t a a t t c g g t c t g t a c t c c t a g t t t g a g g c a t a c c t t c c g t t g c

360 20 25 30 35 VNGHHFVIDGDGTGKPYEGK mEos4a VNGHHFVIDGDGTGKPYEGK YVPVVKHDVSITCALRILSF t a a a c g g g c a c c a c t t t g t g a t c g a c g g a g a t g g t a c a g g c a a g c c t t a t g a g g g a a a a c a t t t g c c c g t g g t g a a a c a c t a g c t g c c t c t a c c a t g t c c g t t c g g a a t a c t c c c t t t t g

EcoRVEciI 420 40 45 50 55 QTMDLEVKEGGPLPFAFDIL mEos4a QTMDLEVKEGGPLPFAFDIL LGHIKFDFLASRQRKGKIDQ a g a c c a t g g a t c t t g a a g t c a a a g a g g g c g g a c c t c t g c c t t t t g c c t t t g a t a t c c t g a t c t g g t a c c t a g a a c t t c a g t t t c t c c c g c c t g g a g a c g g a a a a c g g a a a c t a t a g g a c t

BtsαIBtsI 480 60 65 70 75 TTAFHYGNRVFVKYPDNIQD mEos4a TTAFHYGNRVFVKYPDNIQD GSCEMVAVPYENFIWVVYLV c c a c t g c a t t c c a t t a c g g c a a c a g g g t a t t c g t t a a a t a t c c a g a c a a c a t a c a a g a c t g g t g a c g t a a g g t a a t g c c g t t g t c c c a t a a g c a a t t t a t a g g t c t g t t g t a t g t t c t g a

Bsu36I 540 80 85 90 95 YFKQSFPKGYSWERSLTFED mEos4a YFKQSFPKGYSWERSLTFED IKLLRKRLPIRPFSAQSEFV a t t t t a a g c a g t c g t t t c c t a a g g g g t a t t c g t g g g a a c g a a g c t t g a c t t t c g a a g a c g t a a a a t t c g t c a g c a a a g g a t t c c c c a t a a g c a c c c t t g c t t c g a a c t g a a a g c t t c t g c

600 100 105 110 115 GGICYARNDITMEGDTFYNK mEos4a GGICYARNDITMEGDTFYNK PANAIGSVVYCHFPVSEIIF g g g g c a t t t g c t a t g c c a g a a a c g a c a t a a c a a t g g a a g g g g a c a c t t t c t a t a a t a a a g c c c c g t a a a c g a t a c g g t c t t t g c t g t a t t g t t a c c t t c c c c t g t g a a a g a t a t t a t t t c

660 120 125 130 135 VRFYGTNFPANGPVMQKKTL mEos4a VRFYGTNFPANGPVMQKKTL NSKITGVKGGITWNHLLLRQ t t c g a t t t t a t g g t a c c a a c t t t c c c g c c a a t g g t c c a g t t a t g c a g a a g a a g a c g c t g a a a g c t a a a a t a c c a t g g t t g a a a g g g c g g t t a c c a g g t c a a t a c g t c t t c t t c t g c g a c t

720 140 145 150 155 KWEPSTEKMYVRDGVLTGDI mEos4a KWEPSTEKMYVRDGVLTGDI FPLGGSLFHIHTISHQRTIN a a t g g g a g c c c t c c a c t g a g a a a a t g t a t g t g c g t g a t g g a g t g c t g a c g g g t g a t a t t c t t a c c c t c g g g a g g t g a c t c t t t t a c a t a c a c g c a c t a c c t c a c g a c t g c c c a c t a t a a g

Eco57MIAcuINdeI 780 160 165 170 175 HMALLLEGNAHYRCDFRTTY mEos4a HMALLLEGNAHYRCDFRTTY MHSQQKFSIGM a t a t g g c t t t g t t g c t t g a a g g a a a t g c c c a t t a c c g a t g t g a c t t c a g a a c t a c t t a c a t a t a c c g a a a c a a c g a a c t t c c t t t a c g g g t a a t g g c t a c a c t g a a g t c t t g a t g a a t g t

PflMIBpu10I 840 180 185 190 195 KAKEKGVKLPGYHFVDHAIE mEos4a KAKEKGVKLPGYHFVDHAIE a a g c t a a g g a g a a g g g t g t c a a g t t a c c a g g c t a c c a c t t t g t g g a c c a c g c c a t t g a g a t t c g a t t c c t c t t c c c a c a g t t c a a t g g t c c g a t g g t g a a a c a c c t g g t g c g g t a a c t c t

SphINspI 900 200 205 210 215 ILSHDKDYNKVKLYEHAVAH mEos4a ILSHDKDYNKVKLYEHAVAH t t t t a a g c c a t g a c a a a g a t t a c a a c a a g g t t a a g c t g t a t g a g c a t g c t g t t g c t c a t t a a a a t t c g g t a c t g t t t c t a a t g t t g t t c c a a t t c g a c a t a c t c g t a c g a c a a c g a g t a a

BsaBI*XbaI*EagIEaeINotI 960 220 225 SGLPDNARR* mEos4a SGLPDNARR* c t g g a t t g c c t g a c a a t g c c a g a c g a t a a g c g g c c g c g a c t c t a g a t c a t a a t c a g c c a t g a c c t a a c g g a c t g t t a c g g t c t g c t a t t c g c c g g c g c t g a g a t c t a g t a t t a g t c g g t a

DraI 1020 a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c

End (1065) HpaIMfeI 3ʹ 5ʹ 1065 a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.