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Sequence Analyzer: pIcaRA-I1


Map View

pBRrevBam (5600 .. 5619) NaeI (5565) NgoMIV (5563) PpuMI (5410) NdeI (5373) StyI (5330) BsaAI - SnaBI (5031) Tet-R (4867 .. 4886) HpaI (4549) BciVI (4500) ApaI (4254) PspOMI (4250) BstEII (4224) AflIII - MluI (4043) BsiHKAI (4027) ApaLI (4023) LacI-R (3712 .. 3731) AclI (3705) AscI (3614) StuI (3571) KpnI (3553) Acc65I (3549) EGFP-C (3479 .. 3500) EXFP-R (3132 .. 3151) EGFP-N (2871 .. 2892) BbvCI (37) NheI (43) BmtI (47) AccI (55) BstZ17I (56) XmnI (99) BsrBI (217) BtgI (292) SacII (295) pBR322ori-F (388 .. 407) HindIII (1202) BsmBI - Esp3I (1304) AsiSI (1326) TspMI - XmaI (1448) SmaI (1450) Kan-R (1664 .. 1683) pENTR-R (1748 .. 1767) lac operator (symmetric) BlpI (2340) MscI (2380) BsaBI * (2395) pIcaRA-I1 5676 bp

Sequence View

AccIBstZ17IBmtINheIBbvCI 60 g c t t c c t c g c t c a c t g a c t c g c t g c a c g a g g c a g a c c t c a g c g c t a g c g g a g t g t a t a c t c g a a g g a g c g a g t g a c t g a g c g a c g t g c t c c g t c t g g a g t c g c g a t c g c c t c a c a t a t g a

XmnI 120 g g c t t a c t a t g t t g g c a c t g a t g a g g g t g t c a g t g a a g t g c t t c a t g t g g c a g g a g a a a a c c g a a t g a t a c a a c c g t g a c t a c t c c c a c a g t c a c t t c a c g a a g t a c a c c g t c c t c t t t t

180 a a g g c t g c a c c g g t g c g t c a g c a g a a t a t g t g a t a c a g g a t a t a t t c c g c t t c c t c g c t c t t c c g a c g t g g c c a c g c a g t c g t c t t a t a c a c t a t g t c c t a t a t a a g g c g a a g g a g c g a g

BsrBI 240 a c t g a c t c g c t a c g c t c g g t c g t t c g a c t g c g g c g a g c g g a a a t g g c t t a c g a a c g g g g c t g a c t g a g c g a t g c g a g c c a g c a a g c t g a c g c c g c t c g c c t t t a c c g a a t g c t t g c c c c g

BtgISacII 300 g g a g a t t t c c t g g a a g a t g c c a g g a a g a t a c t t a a c a g g g a a g t g a g a g g g c c g c g g c a a c c t c t a a a g g a c c t t c t a c g g t c c t t c t a t g a a t t g t c c c t t c a c t c t c c c g g c g c c g t t

360 p15A ori a g c c g t t t t t c c a t a g g c t c c g c c c c c c t g a c a a g c a t c a c g a a a t c t g a c g c t c a a a t c t c g g c a a a a a g g t a t c c g a g g c g g g g g g a c t g t t c g t a g t g c t t t a g a c t g c g a g t t t a g

420 p15A ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g t g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c t g g c g g c t c c c t t c a c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g a c c g c c g a g g g a

480 p15A ori c g t g c g c t c t c c t g t t c c t g c c t t t c g g t t t a c c g g t g t c a t t c c g c t g t t a t g g c c g c g g c a c g c g a g a g g a c a a g g a c g g a a a g c c a a a t g g c c a c a g t a a g g c g a c a a t a c c g g c g c

540 p15A ori t t t g t c t c a t t c c a c g c c t g a c a c t c a g t t c c g g g t a g g c a g t t c g c t c c a a g c t g g a c t a a a c a g a g t a a g g t g c g g a c t g t g a g t c a a g g c c c a t c c g t c a a g c g a g g t t c g a c c t g a

600 p15A ori g t a t g c a c g a a c c c c c c g t t c a g t c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g c a t a c g t g c t t g g g g g g c a a g t c a g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c

660 p15A ori a g t c c a a c c c g g a a a g a c a t g c a a a a g c a c c a c t g g c a g c a g c c a c t g g t a a t t g a t t t a t c a g g t t g g g c c t t t c t g t a c g t t t t c g t g g t g a c c g t c g t c g g t g a c c a t t a a c t a a a t

720 p15A ori g a g g a g t t a g t c t t g a a g t c a t g c g c c g g t t a a g g c t a a a c t g a a a g g a c a a g t t t t g g t c t c c t c a a t c a g a a c t t c a g t a c g c g g c c a a t t c c g a t t t g a c t t t c c t g t t c a a a a c c a

780 p15A ori g a c t g c g c t c c t c c a a g c c a g t t a c c t c g g t t c a a a g a g t t g g t a g c t c a g a g a a c c t t c c t g a c g c g a g g a g g t t c g g t c a a t g g a g c c a a g t t t c t c a a c c a t c g a g t c t c t t g g a a g

840 p15A ori g a a a a a c c g c c c t g c a a g g c g g t t t t t t c g t t t t c a g a g c a a g a g a t t a c g c g c a g a c c a c t t t t t g g c g g g a c g t t c c g c c a a a a a a g c a a a a g t c t c g t t c t c t a a t g c g c g t c t g g t

900 p15A ori a a a c g a t c t c a a g a a g a t c a t c t t a t t a a g g g g t c t g a c g c t c a g t g g a a c g a a a a a t c a t t t g c t a g a g t t c t t c t a g t a g a a t a a t t c c c c a g a c t g c g a g t c a c c t t g c t t t t t a g t

960 270 *FFEDLM KanR 270 *FFEDLM KanR *FFEDLM a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c t t a g a a a a a c t c a t c g a g c a t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g a a t c t t t t t g a g t a g c t c g t

1020 265 260 255 250 LHFQLKNMDPNDIGYKQFLR KanR 265 260 255 250 LHFQLKNMDPNDIGYKQFLR KanR LHFQLKNMDPNDIGYKQFLR t c a a a t g a a a c t g c a a t t t a t t c a t a t c a g g a t t a t c a a t a c c a t a t t t t t g a a a a a g c c a g t t t a c t t t g a c g t t a a a t a a g t a t a g t c c t a a t a g t t a t g g t a t a a a a a c t t t t t c g g

1080 245 240 235 230 KQLSPSFEGLCNWLIALDQY KanR 245 240 235 230 KQLSPSFEGLCNWLIALDQY KanR KQLSPSFEGLCNWLIALDQY g t t t c t g t a a t g a a g g a g a a a a c t c a c c g a g g c a g t t c c a t a g g a t g g c a a g a t c c t g g t c a a a g a c a t t a c t t c c t c t t t t g a g t g g c t c c g t c a a g g t a t c c t a c c g t t c t a g g a c c a

1140 225 220 215 210 RDAIGVRGVDICGILKGEDF KanR 225 220 215 210 RDAIGVRGVDICGILKGEDF KanR RDAIGVRGVDICGILKGEDF a t c g g t c t g c g a t t c c g a c t c g t c c a a c a t c a a t a c a a c c t a t t a a t t t c c c c t c g t c a a t a g c c a g a c g c t a a g g c t g a g c a g g t t g t a g t t a t g t t g g a t a a t t a a a g g g g a g c a g t t

1200 205 200 195 190 ILNDLSFDGHTVVSDPSFPL KanR 205 200 195 190 ILNDLSFDGHTVVSDPSFPL KanR ILNDLSFDGHTVVSDPSFPL a a a t a a g g t t a t c a a g t g a g a a a t c a c c a t g a g t g a c g a c t g a a t c c g g t g a g a a t g g c a t t t a t t c c a a t a g t t c a c t c t t t a g t g g t a c t c a c t g c t g a c t t a g g c c a c t c t t a c c g t

HindIII 1260 185 180 175 170 LKHMEKWVQEVPWGNREDDF KanR 185 180 175 170 LKHMEKWVQEVPWGNREDDF KanR LKHMEKWVQEVPWGNREDDF a a a g c t t a t g c a t t t c t t t c c a g a c t t g t t c a a c a g g c c a g c c a t t a c g c t c g t c a t c a a t t t c g a a t a c g t a a a g a a a g g t c t g a a c a a g t t g t c c g g t c g g t a a t g c g a g c a g t a g t t

BsmBIEsp3I 1320 165 160 155 150 DSADVLGNNMRSQAQALRFV KanR 165 160 155 150 DSADVLGNNMRSQAQALRFV KanR DSADVLGNNMRSQAQALRFV a a t c a c t c g c a t c a a c c a a a c c g t t a t t c a t t c g t g a t t g c g c c t g a g c g a g a c g a a a t a t t a g t g a g c g t a g t t g g t t t g g c a a t a a g t a a g c a c t a a c g c g g a c t c g c t c t g c t t t a t

AsiSI 1380 145 140 135 130 RDSNFPCNCVPISHLRRLFV KanR 145 140 135 130 RDSNFPCNCVPISHLRRLFV KanR RDSNFPCNCVPISHLRRLFV c g c g a t c g c t g t t a a a a g g a c a a t t a c a a a c a g g a a t c g a a t g c a a c c g g c g c a g g a a c a g c g c t a g c g a c a a t t t t c c t g t t a a t g t t t g t c c t t a g c t t a c g t t g g c c g c g t c c t t g t

1440 125 120 115 110 ALADVINEGSDPYEELVQFA KanR 125 120 115 110 ALADVINEGSDPYEELVQFA KanR ALADVINEGSDPYEELVQFA c t g c c a g c g c a t c a a c a a t a t t t t c a c c t g a a t c a g g a t a t t c t t c t a a t a c c t g g a a t g g a c g g t c g c g t a g t t g t t a t a a a a g t g g a c t t a g t c c t a t a a g a a g a t t a t g g a c c t t a c

TspMIXmaISmaI 1500 105 100 95 90 TKGPIATTLLWADDPTRIFH KanR 105 100 95 90 TKGPIATTLLWADDPTRIFH KanR TKGPIATTLLWADDPTRIFH c t g t t t t c c c g g g g a t c g c a g t g g t g a g t a a c c a t g c a t c a t c a g g a g t a c g g a t a a a a t g a c a a a a g g g c c c c t a g c g t c a c c a c t c a t t g g t a c g t a g t a g t c c t c a t g c c t a t t t t a

1560 85 80 75 70 KITPLPMFETLWNLRVMEDT KanR 85 80 75 70 KITPLPMFETLWNLRVMEDT KanR KITPLPMFETLWNLRVMEDT g c t t g a t g g t c g g a a g a g g c a t a a a t t c c g t c a g c c a g t t t a g t c t g a c c a t c t c a t c t g c g a a c t a c c a g c c t t c t c c g t a t t t a a g g c a g t c g g t c a a a t c a g a c t g g t a g a g t a g a c

1620 65 60 55 50 VDNAVSGKGHKLFLEPADPK KanR 65 60 55 50 VDNAVSGKGHKLFLEPADPK KanR VDNAVSGKGHKLFLEPADPK t a a c a t c a t t g g c a a c g c t a c c t t t g c c a t g t t t c a g a a a c a a c t c t g g c g c a t c g g g c t a t t g t a g t a a c c g t t g c g a t g g a a a c g g t a c a a a g t c t t t g t t g a g a c c g c g t a g c c c g a

1680 45 40 35 30 GYLRYITAGSQGVNDRAWKY KanR 45 40 35 30 GYLRYITAGSQGVNDRAWKY KanR A T C G C G A G C C C A T T T A T Kan-R GYLRYITAGSQGVNDRAWKY t c c c a t a c a a t c g a t a g a t t g t c g c a c c t g a t t g c c c g a c a t t a t c g c g a g c c c a t t t a t a g g g t a t g t t a g c t a t c t a a c a g c g t g g a c t a a c g g g c t g t a a t a g c g c t c g g g t a a a t a

1740 25 20 15 10 GYLDADMNSNLRPRSCSTER KanR 25 20 15 10 GYLDADMNSNLRPRSCSTER KanR A C C Kan-R GYLDADMNSNLRPRSCSTER a c c c a t a t a a a t c a g c a t c c a t g t t g g a a t t t a a t c g c g g c c t c g a g c a a g a c g t t t c c c t g g g t a t a t t t a g t c g t a g g t a c a a c c t t a a a t t a g c g c c g g a g c t c g t t c t g c a a a g g g

1800 5 1 QIHSM KanR 5 1 QIHSM KanR A T G G C T C A T A A C A C C C C T T G pENTR-R QIHSM g t t g a a t a t g g c t c a t a a c a c c c c t t g t a t t a c t g t t t a t g t a a g c a g a c a g t t t t a t t g c a a c t t a t a c c g a g t a t t g t g g g g a a c a t a a t g a c a a a t a c a t t c g t c t g t c a a a a t a a c

1860 t t c a t g a t g a t a t a t t t t t a t c t t g t g c a a t g t a c a t c a g a g a t t t t g a g a c a c a a g g a g a a g t a c t a c t a t a t a a a a a t a g a a c a c g t t a c a t g t a g t c t c t a a a a c t c t g t g t t c c t c

1920 -35 -10 tac promoter lac operator (symmetric) a a c g a t c g t t g g c t g t g t t g a c a a t t a a t c a t c g g c t c g t a t a a t g t g t g g a a t t g t g a g t t g c t a g c a a c c g a c a c a a c t g t t a a t t a g t a g c c g a g c a t a t t a c a c a c c t t a a c a c t c

1980 lac operator (symmetric) c g c t c a c a a t t a g c c c c a t a g g g t g g t g t g t a c c a c c c c t g a t g a g t c c a a a a g g a c g a a g c g a g t g t t a a t c g g g g t a t c c c a c c a c a c a t g g t g g g g a c t a c t c a g g t t t t c c t g c t t

2040 a t g g g g c c t c t a c a a a t a a t t t t g t t t a a a t t g c t a t g g a c t a t g t t t c a a a g t g a g a a t t a c c c c g g a g a t g t t t a t t a a a a c a a a t t t a a c g a t a c c t g a t a c a a a g t t t c a c t c t t a

2100 a c t a g g t g a a a g a c a a a a t t a t c g a t a a c g c c a t c a c c c t g t t t a g c g a a a a a g g t t a t g t g a t c c a c t t t c t g t t t t a a t a g c t a t t g c g g t a g t g g g a c a a a t c g c t t t t t c c a a t a c

2160 a c g g c a c c a c c c t g g a t g a t a t t g c a a a a a g c g t g a a c a t c a a a a a a g c c a g c c t g t a t t t g c c g t g g t g g g a c c t a c t a t a a c g t t t t t c g c a c t t g t a g t t t t t t c g g t c g g a c a t a a

2220 a t c a c t t t g a t a g c a a a a a a a g c a t c t a c g a g c a g a g c g t t a a a t g c t g t t t c g a t t a t c t a g t g a a a c t a t c g t t t t t t t c g t a g a t g c t c g t c t c g c a a t t t a c g a c a a a g c t a a t a g

2280 t g a a c a a c a t c a t c a t g a t g a a c c a g a a c a a a a g c a a c t a t a g c a t c g a t g c c c t g t a t c a c t t g t t g t a g t a g t a c t a c t t g g t c t t g t t t t c g t t g a t a t c g t a g c t a c g g g a c a t a g

2340 a g t t t c t g t t t g a g t t c a t c t t c g a t a t c g a g g a a c g c t a t a t t c g t a t g t a t g t t c a g c t c a a a g a c a a a c t c a a g t a g a a g c t a t a g c t c c t t g c g a t a t a a g c a t a c a t a c a a g t c g

BsaBI*MscIBlpI 2400 t g a g c a a c a c a c c g g a a g a a t t t t c a g g t a a c a t t t a t g g c c a g a t c c a g g a t c t g a a t c a c t c g t t g t g t g g c c t t c t t a a a a g t c c a t t g t a a a t a c c g g t c t a g g t c c t a g a c t t a g

2460 a g a g c c t g a g c a a a g a a a t c g c c a a a t t c t a t g a c g a a a g c a a a a t c a a a a t g a c c a a a g t c t c g g a c t c g t t t c t t t a g c g g t t t a a g a t a c t g c t t t c g t t t t a g t t t t a c t g g t t t c

2520 a g g a c t t c c a g a a t c t g a t t c t g c t g t t t c t g g a a a g c t g g t a t c t g a a a g c c a g c t t t a t c c t g a a g g t c t t a g a c t a a g a c g a c a a a g a c c t t t c g a c c a t a g a c t t t c g g t c g a a a t

2580 g c c a g a a a t t t g g t g c a g t t g a a g a a a g c a a a a g c c a g t t t a a a g a t g a g g t t t a t a g c c c g g t c t t t a a a c c a c g t c a a c t t c t t t c g t t t t c g g t c a a a t t t c t a c t c c a a a t a t c g g

2640 t g c t g a a c a t c t t t c t g a a g a a a t a a a c a a t t t t c g a a a a a a c c c g c t t c g g c g g g t t t t a c g a c t t g t a g a a a g a c t t c t t t a t t t g t t a a a a g c t t t t t t g g g c g a a g c c g c c c a a a a

2700 t t t a t a g c t a a a a t a c t g t c a a c t c a t a a g a t t c t g a t t c g t t a c c a a t t g a c a a t t c a c a a a t a t c g a t t t t a t g a c a g t t g a g t a t t c t a a g a c t a a g c a a t g g t t a a c t g t t a a g t g

2760 sTRSV HHRz c t a c c t t t c g t t a g g t t a g g t t g t a g c t g t c a c c g g a t g t g c t t t c c g g t c t g a t g a g t c g a t g g a a a g c a a t c c a a t c c a a c a t c g a c a g t g g c c t a c a c g a a a g g c c a g a c t a c t c a g

2820 sTRSV HHRz c g t g a g g a c g a a a c a g c c t c t a c a a a t a a t t t t g t t t a a t a c t a g a g a a a g a g g g g a a a t g c a c t c c t g c t t t g t c g g a g a t g t t t a t t a a a a c a a a t t a t g a t c t c t t t c t c c c c t t t a

2880 1 M 1a V 5 10 15 SKGEELFTGVVPILVE EYFP G A C C A G C T C G EGFP-N MVSKGEELFTGVVPILVE a c t a g a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g a t c t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g

2940 20 25 30 35 LDGDVNGHKFSVSGEGEGDA EYFP A C C T G C C G C T G C EGFP-N LDGDVNGHKFSVSGEGEGDA t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t

3000 40 45 50 55 TYGKLTLKFICTTGKLPVPW EYFP TYGKLTLKFICTTGKLPVPW c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c a g g c a a g c t g c c c g t g c c c t g g c g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g t c c g t t c g a c g g g c a c g g g a c c g

3060 60 65 70 75 PTLVTTFGYGLQCFARYPDH EYFP PTLVTTFGYGLQCFARYPDH c c a c c c t c g t g a c c a c c t t c g g c t a c g g c c t g c a a t g c t t c g c c c g c t a c c c c g a c c a c a g g t g g g a g c a c t g g t g g a a g c c g a t g c c g g a c g t t a c g a a g c g g g c g a t g g g g c t g g t g t

3120 80 85 90 95 MKLHDFFKSAMPEGYVQERT EYFP MKLHDFFKSAMPEGYVQERT t g a a g c t g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a a c t t c g a c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t

3180 100 105 110 115 IFFKDDGNYKTRAEVKFEGD EYFP C T G C T G C C G T T G A T G T T C T G EXFP-R IFFKDDGNYKTRAEVKFEGD t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t

3240 120 125 130 135 TLVNRIELKGIDFKEDGNIL EYFP TLVNRIELKGIDFKEDGNIL c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c

3300 140 145 150 155 GHKLEYNYNSHNVYIMADKQ EYFP GHKLEYNYNSHNVYIMADKQ g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t

3360 160 165 170 175 KNGIKVNFKIRHNIEDGSVQ EYFP KNGIKVNFKIRHNIEDGSVQ a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g

3420 180 185 190 195 LADHYQQNTPIGDGPVLLPD EYFP LADHYQQNTPIGDGPVLLPD t c g c c g a c c a c t a c c a g c a g a a c a c c c c a a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a g c g g c t g g t g a t g g t c g t c t t g t g g g g t t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t

3480 200 205 210 215 NHYLSYQSALSKDPNEKRDH EYFP C A EGFP-C NHYLSYQSALSKDPNEKRDH a c c a c t a c c t t a g c t a c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t g a t g g a a t c g a t g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t

3540 220 225 230 235 MVLLEFVTAAGITLGMDELY EYFP T G G T C C T G C T G G A G T T C G T G EGFP-C MVLLEFVTAAGITLGMDELY t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t

StuIAcc65IKpnI 3600 K* EYFP K* a g t a a c t c g g t a c c a a a t t c c a g a a a a g a g g c c t c c c g a a a g g g g g g c c t t t t t t c g t t t t c a t t g a g c c a t g g t t t a a g g t c t t t t c t c c g g a g g g c t t t c c c c c c g g a a a a a a g c a a a

AscI 3660 t g g t c c a a t g g c g g c g c g c c a t c g a a t g g c g c a a a a c c t t t c g c g g t a t g g c a t g a t a g c a c c a g g t t a c c g c c g c g c g g t a g c t t a c c g c g t t t t g g a a a g c g c c a t a c c g t a c t a t c g

AclI 3720 1 5 MKPVTLYDV lacI T G C T A C A G C LacI-R MKPVTLYDV g c c c g g a a g a g a g t c a a t t c a g g g t g g t g a a t a t g a a a c c a g t a a c g t t a t a c g a t g t c g c g g g c c t t c t c t c a g t t a a g t c c c a c c a c t t a t a c t t t g g t c a t t g c a a t a t g c t a c a g c

3780 10 15 20 25 AEYAGVSYQTVSRVVNQASH lacI G T C T C A T A C G G LacI-R AEYAGVSYQTVSRVVNQASH c a g a g t a t g c c g g t g t c t c t t a t c a g a c c g t t t c c c g c g t g g t g a a c c a g g c c a g c c a c g g t c t c a t a c g g c c a c a g a g a a t a g t c t g g c a a a g g g c g c a c c a c t t g g t c c g g t c g g t g c

3840 30 35 40 45 VSAKTREKVEAAMAELNYIP lacI VSAKTREKVEAAMAELNYIP t t t c t g c g a a a a c g c g g g a a a a a g t g g a a g c g g c g a t g g c g g a g c t g a a t t a c a t t c c c a a a a g a c g c t t t t g c g c c c t t t t t c a c c t t c g c c g c t a c c g c c t c g a c t t a a t g t a a g g g t

3900 50 55 60 65 NRVAQQLAGKQSLLIGVATS lacI NRVAQQLAGKQSLLIGVATS a c c g c g t g g c a c a a c a a c t g g c g g g c a a a c a g t c g t t g c t g a t t g g c g t t g c c a c c t c c a t g g c g c a c c g t g t t g t t g a c c g c c c g t t t g t c a g c a a c g a c t a a c c g c a a c g g t g g a g g t

3960 70 75 80 85 SLALHAPSQIVAAIKSRADQ lacI SLALHAPSQIVAAIKSRADQ g t c t g g c c c t g c a c g c g c c g t c g c a a a t t g t c g c g g c g a t t a a a t c t c g c g c c g a t c a a c c a g a c c g g g a c g t g c g c g g c a g c g t t t a a c a g c g c c g c t a a t t t a g a g c g c g g c t a g t t g

4020 90 95 100 105 LGASVVVSMVERSGVEACKA lacI LGASVVVSMVERSGVEACKA t g g g t g c c a g c g t g g t g g t g t c g a t g g t a g a a c g a a g c g g c g t c g a a g c c t g t a a a g c g g a c c c a c g g t c g c a c c a c c a c a g c t a c c a t c t t g c t t c g c c g c a g c t t c g g a c a t t t c g c c

AflIIIMluIApaLIBsiHKAI 4080 110 115 120 125 AVHNLLAQRVSGLIINYPLD lacI AVHNLLAQRVSGLIINYPLD c g g t g c a c a a t c t t c t c g c g c a a c g c g t c a g t g g g c t g a t c a t t a a c t a t c c g c t g g a t g g c c a c g t g t t a g a a g a g c g c g t t g c g c a g t c a c c c g a c t a g t a a t t g a t a g g c g a c c t a c

4140 130 135 140 145 DQDAIAVEAACTNVPALFLD lacI DQDAIAVEAACTNVPALFLD a c c a g g a t g c c a t t g c t g t g g a a g c t g c c t g c a c t a a t g t t c c g g c g t t a t t t c t t g a t g t g g t c c t a c g g t a a c g a c a c c t t c g a c g g a c g t g a t t a c a a g g c c g c a a t a a a g a a c t a c

4200 150 155 160 165 VSDQTPINSIIFSHEDGTRL lacI VSDQTPINSIIFSHEDGTRL t c t c t g a c c a g a c a c c c a t c a a c a g t a t t a t t t t c t c c c a t g a g g a c g g t a c g c g a c t g g a g a g a c t g g t c t g t g g g t a g t t g t c a t a a t a a a a g a g g g t a c t c c t g c c a t g c g c t g a c c

PspOMIApaIBstEII 4260 170 175 180 185 GVEHLVALGHQQIALLAGPL lacI GVEHLVALGHQQIALLAGPL g c g t g g a g c a t c t g g t c g c a t t g g g t c a c c a g c a a a t c g c g c t g t t a g c g g g c c c a t t a a c g c a c c t c g t a g a c c a g c g t a a c c c a g t g g t c g t t t a g c g c g a c a a t c g c c c g g g t a a t t

4320 190 195 200 205 SSVSARLRLAGWHKYLTRNQ lacI SSVSARLRLAGWHKYLTRNQ g t t c t g t c t c g g c g c g t c t g c g t c t g g c t g g c t g g c a t a a a t a t c t c a c t c g c a a t c a a a c a a g a c a g a g c c g c g c a g a c g c a g a c c g a c c g a c c g t a t t t a t a g a g t g a g c g t t a g t t t

4380 210 215 220 225 IQPIAEREGDWSAMSGFQQT lacI IQPIAEREGDWSAMSGFQQT t t c a g c c g a t a g c g g a a c g g g a a g g c g a c t g g a g t g c c a t g t c c g g t t t t c a a c a a a c c a a a g t c g g c t a t c g c c t t g c c c t t c c g c t g a c c t c a c g g t a c a g g c c a a a a g t t g t t t g g t

4440 230 235 240 245 MQMLNEGIVPTAMLVANDQM lacI MQMLNEGIVPTAMLVANDQM t g c a a a t g c t g a a t g a g g g c a t c g t t c c c a c t g c g a t g c t g g t t g c c a a c g a t c a g a t g g a c g t t t a c g a c t t a c t c c c g t a g c a a g g g t g a c g c t a c g a c c a a c g g t t g c t a g t c t a c c

BciVI 4500 250 255 260 265 ALGAMRAITESGLRVGADIS lacI ALGAMRAITESGLRVGADIS c g c t g g g c g c a a t g c g c g c c a t t a c c g a g t c c g g g c t g c g c g t t g g t g c g g a t a t c t c g g g c g a c c c g c g t t a c g c g c g g t a a t g g c t c a g g c c c g a c g c g c a a c c a c g c c t a t a g a g c c

HpaI 4560 270 275 280 285 VVGYDDTEDSSCYIPPLTTI lacI VVGYDDTEDSSCYIPPLTTI t a g t g g g a t a c g a c g a t a c c g a a g a t a g c t c a t g t t a t a t c c c g c c g t t a a c c a c c a t c a a t c a c c c t a t g c t g c t a t g g c t t c t a t c g a g t a c a a t a t a g g g c g g c a a t t g g t g g t a g t

4620 290 295 300 305 KQDFRLLGQTSVDRLLQLSQ lacI KQDFRLLGQTSVDRLLQLSQ a a c a g g a t t t t c g c c t g c t g g g g c a a a c c a g c g t g g a c c g c t t g c t g c a a c t c t c t c a g g t t g t c c t a a a a g c g g a c g a c c c c g t t t g g t c g c a c c t g g c g a a c g a c g t t g a g a g a g t c c

4680 310 315 320 325 GQAVKGNQLLPVSLVKRKTT lacI GQAVKGNQLLPVSLVKRKTT g c c a g g c g g t g a a g g g c a a t c a g c t g t t g c c a g t c t c a c t g g t g a a a a g a a a a a c c a c c c c g g t c c g c c a c t t c c c g t t a g t c g a c a a c g g t c a g a g t g a c c a c t t t t c t t t t t g g t g g g

4740 330 335 340 345 LAPNTQTASPRALADSLMQL lacI LAPNTQTASPRALADSLMQL t g g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g a c c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c

4800 350 355 360 ARQVSRLESGQ* 1 MS lacI TetR ARQVSRLESGQ*MS c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a t a a t c c a g g a g g a a a a a a a t g t c c a g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t a t t a g g t c c t c c t t t t t t t a c a g g t

4860 5 10 15 20 RLDKSKVINSALELLNEVGI TetR RLDKSKVINSALELLNEVGI g a t t a g a t a a a a g t a a a g t g a t t a a c a g c g c a t t a g a g c t g c t t a a t g a g g t c g g a a t c g c t a a t c t a t t t t c a t t t c a c t a a t t g t c g c g t a a t c t c g a c g a a t t a c t c c a g c c t t a g c

4920 25 30 35 40 EGLTTRKLAQKLGVEQPTLY TetR A T T G T T G G G C A T T T G A G C G G Tet-R EGLTTRKLAQKLGVEQPTLY a a g g t t t a a c a a c c c g t a a a c t c g c c c a g a a g c t a g g t g t a g a g c a g c c t a c a t t g t a t t t t c c a a a t t g t t g g g c a t t t g a g c g g g t c t t c g a t c c a c a t c t c g t c g g a t g t a a c a t a a

4980 45 50 55 60 WHVKNKRALLDALAIEMLDR TetR WHVKNKRALLDALAIEMLDR g g c a t g t a a a a a a t a a g c g g g c t t t g c t c g a c g c c t t a g c c a t t g a g a t g t t a g a t a g g c c c g t a c a t t t t t t a t t c g c c c g a a a c g a g c t g c g g a a t c g g t a a c t c t a c a a t c t a t c c g

SnaBIBsaAI 5040 65 70 75 80 HHTHFCPLEGESWQDFLRNN TetR HHTHFCPLEGESWQDFLRNN a c c a t a c t c a c t t t t g c c c t t t a g a a g g g g a a a g c t g g c a a g a t t t t t t a c g t a a t a a c g t g g t a t g a g t g a a a a c g g g a a a t c t t c c c c t t t c g a c c g t t c t a a a a a a t g c a t t a t t g c

5100 85 90 95 100 AKSFRCALLSHRDGAKVHLG TetR AKSFRCALLSHRDGAKVHLG c t a a a a g t t t t a g a t g t g c t t t a c t a a g t c a t c g c g a t g g a g c a a a a g t a c a t t t a g g t a g a t t t t c a a a a t c t a c a c g a a a t g a t t c a g t a g c g c t a c c t c g t t t t c a t g t a a a t c c a t

5160 105 110 115 120 TRPTEKQYETLENQLAFLCQ TetR TRPTEKQYETLENQLAFLCQ c a c g g c c t a c a g a a a a a c a g t a t g a a a c t c t c g a a a a t c a a t t a g c c t t t t t a t g c c a a c g t g c c g g a t g t c t t t t t g t c a t a c t t t g a g a g c t t t t a g t t a a t c g g a a a a a t a c g g t t g

5220 125 130 135 140 QGFSLENALYALSAVGHFTL TetR QGFSLENALYALSAVGHFTL a a g g t t t t t c a c t a g a g a a t g c a t t a t a t g c a c t c a g c g c t g t g g g g c a t t t t a c t t t a g t t c c a a a a a g t g a t c t c t t a c g t a a t a t a c g t g a g t c g c g a c a c c c c g t a a a a t g a a a t c

5280 145 150 155 160 GCVLEDQEHQVAKEERETPT TetR GCVLEDQEHQVAKEERETPT g t t g c g t a t t g g a a g a t c a a g a g c a t c a a g t c g c t a a a g a a g a a a g g g a a a c a c c t a c t a c a a c g c a t a a c c t t c t a g t t c t c g t a g t t c a g c g a t t t c t t c t t t c c c t t t g t g g a t g a t

StyI 5340 165 170 175 180 TDSMPPLLRQAIELFDHQGA TetR TDSMPPLLRQAIELFDHQGA c t g a t a g t a t g c c g c c a t t a t t a c g a c a a g c t a t c g a a t t a t t t g a t c a c c a a g g t g c a g g a c t a t c a t a c g g c g g t a a t a a t g c t g t t c g a t a g c t t a a t a a a c t a g t g g t t c c a c g t c

NdeI 5400 185 190 195 200 EPAFLFGLELIICGLEKQLK TetR EPAFLFGLELIICGLEKQLK a g c c a g c c t t c t t a t t c g g c c t t g a a t t g a t c a t a t g c g g a t t a g a a a a a c a a c t t a a a t t c g g t c g g a a g a a t a a g c c g g a a c t t a a c t a g t a t a c g c c t a a t c t t t t t g t t g a a t t t a

PpuMI 5460 205 CESGS* TetR CESGS* g t g a a a g t g g g t c c t a a t a a t t g g t a a c g a a t c a g a c a a t t g a c g g c t c g a g g g a g t a g c c a c t t t c a c c c a g g a t t a t t a a c c a t t g c t t a g t c t g t t a a c t g c c g a g c t c c c t c a t c g

5520 a t a g g g t t t g c a g a a t c c c t g c t t c g t c c a t t t g a c a g g c a c a t t a t g c a t c g a t g a t a a t a t c c c a a a c g t c t t a g g g a c g a a g c a g g t a a a c t g t c c g t g t a a t a c g t a g c t a c t a t t

NgoMIVNaeI 5580 g c t g t c a a a c a t g a g c a g a t c c t c t a c g c c g g a c g c a t c g t g g c c g g c a t c a c c g g c g c c c g a c a g t t t g t a c t c g t c t a g g a g a t g c g g c c t g c g t a g c a c c g g c c g t a g t g g c c g c g g

5640 G G A T A T A G C G G C T G T A G T G G pBRrevBam a c a g g t g c g g t t g c t g g c g c c t a t a t c g c c g a c a t c a c c g a t g g g g a a g a t c g g g c t c g c t g t c c a c g c c a a c g a c c g c g g a t a t a g c g g c t g t a g t g g c t a c c c c t t c t a g c c c g a g c g

5676 c a c t t c g g g c t c a t g a g c a a a t a t t t t a t c t g a g g t g t g a a g c c c g a g t a c t c g t t t a t a a a a t a g a c t c c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.