Addgene pMLS252 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pMLS252


Map View

3000 2000 1000 End (3077) PvuII (3028) GFP-F (3006 .. 3026) EcoP15I (3001) BsaI (2990) MflI * - BstYI (2979) MfeI (2912) HpaI (2733) PluTI - HaeII (2634) SfoI (2632) NarI (2631) KasI (2630) AgeI - BsrFI (2597) BsgI (2483) BseRI (2362) AatII (2342) ZraI (2340) SacI - BanII - BsiHKAI (2191) Eco53kI (2189) BmgBI (2176) BsaBI (2150) SacII (2104) NspI (2004) PciI (2000) XmnI (1981) BspEI (1961) ClaI - BspDI (1770) BfuAI - PaqCI - BspMI (1745) EaeI (1628) PfoI (1538) BsiEI (1290) MslI (1138) BclI * (1041) SspI (946) Bpu10I (815) HindIII (812) XbaI (661) BstZ17I (560) AccI (559) BsrGI (335) AvaI - XhoI - BsoBI - PaeR7I (272) StyI - NcoI (174) BaeGI - Bme1580I (82) GFP-R (39 .. 67) BpmI (55) Start (0) loxP Factor Xa site loxP pMLS252 3077 bp

Sequence View

BpmIStart (0) 60 C A A C A G G G T T A A G A A C A A C T T A GFP-R g c t c t t c g a t g a g c a a a g g a g a a g a a c t t t t c a c t g g a g t t g t c c c a a t t c t t g t t g a a t c g a g a a g c t a c t c g t t t c c t c t t c t t g a a a a g t g a c c t c a a c a g g g t t a a g a a c a a c t t a

Bme1580IBaeGI 120 A T C T A C C GFP-R t a g a t g g t g a t g t t a a t g g g c a c a a a t t t t c t g t c a g t g g a g a g g g t g a a g g t g a t g c a a a t c t a c c a c t a c a a t t a c c c g t g t t t a a a a g a c a g t c a c c t c t c c c a c t t c c a c t a c g t t

NcoIStyI 180 c a t a c g g a a a a c t t a c c c t t a a a t t t a t t t g c a c t a c t g g a a a a c t a c c t g t t c c a t g g g g t a t g c c t t t t g a a t g g g a a t t t a a a t a a a c g t g a t g a c c t t t t g a t g g a c a a g g t a c c c

240 t a a g t t t a a a c a t a t a t a t a c t a a c t a a c c c t g a t t a t t t a a a t t t t c a g c c a a c a c t t g a t t c a a a t t t g t a t a t a t a t g a t t g a t t g g g a c t a a t a a a t t t a a a a g t c g g t t g t g a a c

PaeR7IBsoBIXhoIAvaI 300 t c a c t a c t t t c t g t t a t g g t g t t c a a t g c t t c t c g a g a t a c c c a g a t c a t a t g a a a c g g c a g t g a t g a a a g a c a a t a c c a c a a g t t a c g a a g a g c t c t a t g g g t c t a g t a t a c t t t g c c g

BsrGI 360 a t g a c t t t t t c a a g a g t g c c a t g c c c g a a g g t t a t g t a c a g g a a a g a a c t a t a t t t t t c a t a c t g a a a a a g t t c t c a c g g t a c g g g c t t c c a a t a c a t g t c c t t t c t t g a t a t a a a a a g t

420 a a g a t g a c g g g a a c t a c a a g a c a c g t a a g t t t a a a c a g t t c g g t a c t a a c t a a c c a t a c a t t c t a c t g c c c t t g a t g t t c t g t g c a t t c a a a t t t g t c a a g c c a t g a t t g a t t g g t a t g t

480 t a t t t a a a t t t t c a g g t g c t g a a g t c a a g t t t g a a g g t g a t a c c c t t g t t a a t a g a a t c g a t a a a t t t a a a a g t c c a c g a c t t c a g t t c a a a c t t c c a c t a t g g g a a c a a t t a t c t t a g c

540 a g t t a a a a g g t a t t g a t t t t a a a g a a g a t g g a a a c a t t c t t g g a c a c a a a t t g g a a t a c a t c a a t t t t c c a t a a c t a a a a t t t c t t c t a c c t t t g t a a g a a c c t g t g t t t a a c c t t a t g t

AccIBstZ17I 600 a c t a t a a c t c a c a c a a t g t a t a c a t c a t g g c a g a c a a a c a a a a g a a t g g a a t c a a a g t t g t g a t a t t g a g t g t g t t a c a t a t g t a g t a c c g t c t g t t t g t t t t c t t a c c t t a g t t t c a a c

660 loxP t a a g t t t a a a c a t g a t t t t a c t a a c a t a a c t t c g t a t a a t g t a t g c t a t a c g a a g t t a t t a t t c a a a t t t g t a c t a a a a t g a t t g t a t t g a a g c a t a t t a c a t a c g a t a t g c t t c a a t a a

XbaI 720 t c t a g a c a t t c t c t a a t g a a a a a a t c t t t c a g t t g a a a t t g a a a a t g a g t t a a a g t t g g a a g a t c t g t a a g a g a t t a c t t t t t t a g a a a g t c a a c t t t a a c t t t t a c t c a a t t t c a a c c t

780 g t t t t t a t t g a a a a c a g a t t t c c g t g t g a t t a g t g t t t t t a g c g a g t g t g a c a g g a c a g c c a a a a a t a a c t t t t g t c t a a a g g c a c a c t a a t c a c a a a a a t c g c t c a c a c t g t c c t g t c g

HindIIIBpu10I 840 g a a a a a a t a t a g a a a c a a g g g g g g a a c t g a a a a g c t t a g g a a t g c a t t g a a c a t g a g a a g c t t t t t t a t a t c t t t g t t c c c c c c t t g a c t t t t c g a a t c c t t a c g t a a c t t g t a c t c t t c

900 g g g a a g g g g a a g g a a c a a a c t a g a c a g g a a t t a t t g g a a t t t a a t c a c a t t t g g a g t t t t c c c t t c c c c t t c c t t g t t t g a t c t g t c c t t a a t a a c c t t a a a t t a g t g t a a a c c t c a a a a

SspI 960 t t t t c t a t t c g a c a g a a t a a t t a t c c a g a a c a t t t t t g t a t t a a a t a t t t a t g c a t c a t a a a a a g a t a a g c t g t c t t a t t a a t a g g t c t t g t a a a a a c a t a a t t t a t a a a t a c g t a g t a t

1020 t g a g t a g t c g g c t t t g t t g t g c a t g a c g a g t t t g t t a t c g a c g a a a t a g a a g c t g t c a g a a c t c a t c a g c c g a a a c a a c a c g t a c t g c t c a a a c a a t a g c t g c t t t a t c t t c g a c a g t c t

BclI* 1080 a c g a g t c t c g t t t g g a t t g t t g a t c a t g t c g t c c a c t g a a a a a g a g a t t a g t c t t t t g a a t g c t c a g a g c a a a c c t a a c a a c t a g t a c a g c a g g t g a c t t t t t c t c t a a t c a g a a a a c t t

MslI 1140 t t g t a c t t t t t a g a a t a a t g a c t c a c t g a g t t g t t g a g a g a g t t g a g g g a a c t c a t a g a t a a c a t g a a a a a t c t t a t t a c t g a g t g a c t c a a c a a c t c t c t c a a c t c c c t t g a g t a t c t a

1200 a t g t t c a c a g t t g t t t c g t g a a t t c g g a a t a c a g a a t c c g a a t t c a a a g t c a a a a c a c t t t a c a a g t g t c a a c a a a g c a c t t a a g c c t t a t g t c t t a g g c t t a a g t t t c a g t t t t g t g a a

1260 c a g a a g g c g a t c t t t g a a g a a g t g a c g t t c g a t c a t t c g g a a a t g a t g g a t t g g g a t g t c g t c t t c c g c t a g a a a c t t c t t c a c t g c a a g c t a g t a a g c c t t t a c t a c c t a a c c c t a c a g

BsiEI 1320 t c c t a c t t t g a a t t c c a c a g t t g c t c c g a c c g t t t t g a g t t t c a a a a a g t t t g g a g c a a a a g g a t g a a a c t t a a g g t g t c a a c g a g g c t g g c a a a a c t c a a a g t t t t t c a a a c c t c g t t t

1380 t c t a t a a c g c a c g t a t c t t g c c g a c t c c t g t g g c g a c t c a t c a t t c t c c t g a t c g t t c t c a g a t a t t g c g t g c a t a g a a c g g c t g a g g a c a c c g c t g a g t a g t a a g a g g a c t a g c a a g a g

1440 c g g t t t g g c g a t c t c g a a a a g c a c t t g c t c a g t g t c c a g a t c a c g g a t t t g g a a c t t g g t g c c a a a c c g c t a g a g c t t t t c g t g a a c g a g t c a c a g g t c t a g t g c c t a a a c c t t g a a c c a

1500 g a a c t c g a t g t t a t a g a t g t t c g c a g a t g g g g a g c a t a a g a a t c c t a a a t t t a t g t t t t a c t t g a g c t a c a a t a t c t a c a a g c g t c t a c c c c t c g t a t t c t t a g g a t t t a a a t a c a a a a t

PfoI 1560 a a c t g a a a t c c a a a g g g a g c a a g a t a c c t t g a g t g a t t c c c g g a a g t g c t a a a a c g t c g t t t g a c t t t a g g t t t c c c t c g t t c t a t g g a a c t c a c t a a g g g c c t t c a c g a t t t t g c a g c a

1620 t c g g a g t g a t t t g a g c t t t c t t c g c a a g c t c c g a t t c c g t t g t g a t t c c t t g t t c g g t g c a g c c t c a c t a a a c t c g a a a g a a g c g t t c g a g g c t a a g g c a a c a c t a a g g a a c a a g c c a c g

EaeI 1680 t t g g t g g t g g c c g t g g c a t c t g g a a a t a t g g a a a a g t t c a a c a a a a a g a a a a g a g a a a a g a a c c a c c a c c g g c a c c g t a g a c c t t t a t a c c t t t t c a a g t t g t t t t t c t t t t c t c t t t t c

1740 a a t g a a a t c g g a t a t c a a g a g t t a g t t g a g c g g t t t c t c t a g t t t t c t g a g t c t c a c c t g t t a c t t t a g c c t a t a g t t c t c a a t c a a c t c g c c a a a g a g a t c a a a a g a c t c a g a g t g g a c

BspDIClaIBspMIPaqCIBfuAI 1800 c g a c g g g a a g g t c g c c g a g c c g g g t g g a a t c g a t t g t t g t t g c t c g g c t t t c a t a t c g g t g c t g c c c t t c c a g c g g c t c g g c c c a c c t t a g c t a a c a a c a a c g a g c c g a a a g t a t a g c c a

1860 t t g g t t g g a a g c g g c t g a a a a c g g a a a g a a g t g g a a g a a g g a a a a g a g t g t g g t g t g a c a a a c c a a c c t t c g c c g a c t t t t g c c t t t c t t c a c c t t c t t c c t t t t c t c a c a c c a c a c t g t

1920 g g a a a a t g g t a a t t a g a g g g t g c c a a a t a a c c a g c t a t a t t t t g t t t t t t t t t g a a a a c a c c t t t t a c c a t t a a t c t c c c a c g g t t t a t t g g t c g a t a t a a a a c a a a a a a a a a c t t t t g t

BspEI 1980 t t t t t a a a a a g a a a a a t a c g a t a a t g a t a t c a g a t g g a t t t c c g g a a a a c t g g t a t g a a a a a a a a t t t t t c t t t t t a t g c t a t t a c t a t a g t c t a c c t a a a g g c c t t t t g a c c a t a c t t t

NspIPciIXmnI 2040 a a t t t c a a c c t t t t t g a g t a c a t g t a a t c a a a a t a c a c t t t g t a a a t t a t c a t t t t t a t t t t a a a g t t g g a a a a a c t c a t g t a c a t t a g t t t t a t g t g a a a c a t t t a a t a g t a a a a a t a a

2100 *YNSFSVQQS g a a a c t c c a c c a t t t t t c t a t t t a t a a c g c t a a t a a t t t g a a a a a g a a a c c t g t t g c g a a c t t t g a g g t g g t a a a a a g a t a a a t a t t g c g a t t a t t a a a c t t t t t c t t t g g a c a a c g c t t

BsaBISacII 2160 GRPSDWFRIRKPPTISEFRL c c g c g g g g t g a a t c c c a a a a a c g a a t g c g t t t t g g t g g a g t g a t t g a t t c g a a t c g a a g a g g c g c c c c a c t t a g g g t t t t t g c t t a c g c a a a a c c a c c t c a c t a a c t a a g c t t a g c t t c t

Eco53kIBsiHKAIBanIISacIBmgBI 2220 LFLLLRPISLESKVSCCVGV a g a a a a a g a a g a a g a c g t g g a a t a g a g a g c t c a c t c t t a a c c g a g c a g c a c a c a c c g a c a t c t t t t t c t t c t t c t g c a c c t t a t c t c t c g a g t g a g a a t t g g c t c g t c g t g t g t g g c t g t

2280 SFFIFHILTKKKKKKSHNVS g a a a a a a a a a t g a a a t g a a t g a g g g t c t t c t t c t t c t t c t t c t t c g a a t g a t t g a c a g a a c t t t t t t t t t a c t t t a c t t a c t c c c a g a a g a a g a a g a a g a a g a a g c t t a c t a a c t g t c t t

ZraI 2340 IPFLPLNLLSFPLLFSASSS a t g g g a a a a a g a g g a a g a t t g a g a a g g g a a a a a g g a a g g a g a a a a g a a g c a g a a g a a g a c t a c c c t t t t t c t c c t t c t a a c t c t t c c c t t t t t c c t t c c t c t t t t c t t c g t c t t c t t c t g

BseRIAatII 2400 TLSSLFSRFLLPRLDYFYCL g t c a g a g a g g a g a g g a a c g a g c g g a a a a g c a g c g g g c g c a a g t c a t a g a a g t a g c a g a g c c a g t c t c t c c t c t c c t t g c t c g c c t t t t c g t c g c c c g c g t t c a g t a t c t t c a t c g t c t c g

2460 QPSSSVIWSLFSSLTHLPTY t g g g g a g a a g a a g a c a c t a t c c a a g a a a g g a a t g a c g a g a g a g t a t g c a a a g g g g t a t a g a c c c c t c t t c t t c t g t g a t a g g t t c t t t c c t t a c t g c t c t c t c a t a c g t t t c c c c a t a t c

BsgI 2520 PASLIPVSYCIILWSSNFLA g g t g c a g a c a g a a t a g g a a c a g a a t a a c a g a t g a t g a g c c a a g a a g a g t t g a a a a g g g c g c c a c g t c t g t c t t a t c c t t g t c t t a t t g t c t a c t a c t c g g t t c t t c t c a a c t t t t c c c g c

2580 IFKDHLKIQTLKLM a t g a a t t t g t c a t g t a a c t t a a t t t g g g t c a a t t t g a g c a t g a t g a a t t g a a a t c a t c c c t a c t t a a a c a g t a c a t t g a a t t a a a c c c a g t t a a a c t c g t a c t a c t t a a c t t t a g t a g g g

KasINarISfoIHaeIIPluTIBsrFIAgeI 2640 1 IEGR PN Factor Xa site t t g t t g g g a g t t a a t a a c c g g t t t g t t a t c a g a a a c c c t g t a a t a g a a g g g c g c c c t a a c a a c a a c c c t c a a t t a t t g g c c a a a c a a t a g t c t t t g g g a c a t t a t c t t c c c g c g g g a t t g

2700 (in frame with Factor Xa site) FEPIHPGFCQIYQKVVN* t t t g a g c c a a t t c a t c c c g g t t t c t g t c a a a t a t a t c a a a a a g t g g t c a a c t g a c a a a t t a a a c t c g g t t a a g t a g g g c c a a a g a c a g t t t a t a t a g t t t t t c a c c a g t t g a c t g t t t a a

HpaI 2760 g t t t t t g a t a t t a t a a t a a a c a t t t t a t c c g t t a a c a a t t t t c g a a t a c t t t t t a c a a g g c a a a a a c t a t a a t a t t a t t t g t a a a a t a g g c a a t t g t t a a a a g c t t a t g a a a a a t g t t c c

2820 loxP a c t t g g a t a a a t t g g c t c a a a g a t a a c t t c g t a t a a t g t a t g c t a t a c g a a g t t a t t a a c t g a a c c t a t t t a a c c g a g t t t c t a t t g a a g c a t a t t a c a t a c g a t a t g c t t c a a t a a t t g

2880 t a a t c t g a t t t a a a t t t t c a g a a c t t c a a a a t t a g a c a c a a c a t t g a a g a t g g a a g c g t t a t t a g a c t a a a t t t a a a a g t c t t g a a g t t t t a a t c t g t g t t g t a a c t t c t a c c t t c g c a a

MfeI 2940 c a a c t a g c a g a c c a t t a t c a a c a a a a t a c t c c a a t t g g c g a t g g c c c t g t c c t t t t a c c a g t t g a t c g t c t g g t a a t a g t t g t t t t a t g a g g t t a a c c g c t a c c g g g a c a g g a a a a t g g t

BsaIBstYIMflI* 3000 g a c a a c c a t t a c c t g t c c a c a c a a t c t g c c c t t t c g a a a g a t c c c a a c g a a a a g a g a g a c c t g t t g g t a a t g g a c a g g t g t g t t a g a c g g g a a a g c t t t c t a g g g t t g c t t t t c t c t c t g

PvuIIEcoP15I 3060 G G T C C T T C T T G A G T T T G T A A C GFP-F c a c a t g g t c c t t c t t g a g t t t g t a a c a g c t g c t g g g a t t a c a c a t g g c a t g g a t g a a c t a g t g t a c c a g g a a g a a c t c a a a c a t t g t c g a c g a c c c t a a t g t g t a c c g t a c c t a c t t g a t

End (3077) 3077 t a c a a a g g t c g a a g a g c a t g t t t c c a g c t t c t c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.