Addgene pEMS1144 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pEMS1144


Map View

pBRforEco (49 .. 67) pBR322ori-F (18,643 .. 18,662) L4440 (18,392 .. 18,409) pRS-marker (18,292 .. 18,311) pGEX 3' (18,129 .. 18,151) PspXI (18,004) NsiI (15,447) I-SceI (14,169) F1ori-F (12,773 .. 12,794) SalI (12,728) AvrII (127) SbfI (1872) KflI (2080) PmlI (3228) EGFP-N (4088 .. 4109) EXFP-R (4349 .. 4368) EGFP-C (4696 .. 4717) SrfI (4779) SV40 NLS SV40pA-R (5084 .. 5103) PshAI (5420) ZraI (6360) AatII (6362) MreI - SgrAI (6576) BspDI * - ClaI * (6910) BstEII (7393) pEMS1144 20,317 bp

Sequence View

5ʹ 3ʹ 60 A A T A G G C G T A T C pBRforEco c g g c c g c a c g t c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a a t a g g c g t a t c g c c g g c g t g c a g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t t a t c c g c a t a g

120 A C G A G G C pBRforEco a c g a g g c c c t t t c g t c t t c a a g a a t t a a t t c c g a t c a t a t t c a a t a a c c c t t a a t g a a t t t g c t c c g g g a a a g c a g a a g t t c t t a a t t a a g g c t a g t a t a a g t t a t t g g g a a t t a c t t a a

AvrII 180 c g a g c t c c t a g g c c g g c c a c g c g t g g c g c g c c c a g a a g t t c t c a t t c t t g t c t a c g g a t g g c t c g a g g a t c c g g c c g g t g c g c a c c g c g c g g g t c t t c a a g a g t a a g a a c a g a t g c c t a c

240 c c a t t c c t t t c t t t g c c t c t t t g g a g a t t c t a a a g g t c t c t g a a a t c a t t a t t a g a t t a c g g t a a g g a a a g a a a c g g a g a a a c c t c t a a g a t t t c c a g a g a c t t t a g t a a t a a t c t a a t g

300 t g t a t t a c c t t c c t t c c t a c t g g a a t g a a t t c a c c t c c a t g t t t t t t a t t c t g t t g c t t a a c a t a a t g g a a g g a a g g a t g a c c t t a c t t a a g t g g a g g t a c a a a a a a t a a g a c a a c g a a t

360 c c t t t c t t a g c c t t c a t a t a c c t c t t g c g c t t t g a a a t t g a t t t g c g a c t c c t c t t a g c t g g a a a g a a t c g g a a g t a t a t g g a g a a c g c g a a a c t t t a a c t a a a c g c t g a g g a g a a t c g a

420 g a g c c a a t g t t g t g g a g a c a g g g a g t g g c c t g c a g t g c c t g a a a c t g c a g g g c a g c a g c c c t c g g t t a c a a c a c c t c t g t c c c t c a c c g g a c g t c a c g g a c t t t g a c g t c c c g t c g t c g g

480 g c c t c c c a g g a a t g t c c t g g g t c c t t t c c t c c t t t g a g g t c c c c t g c c a t a t g c t c a g a t c g g a g g g t c c t t a c a g g a c c c a g g a a a g g a g g a a a c t c c a g g g g a c g g t a t a c g a g t c t a

540 g g g g c c a g g c a c a g c a a c g g g g g c a c a c c t c c c t t t g g c c c a g c a g t t g g g g t g c t g c c a c c c c g g t c c g t g t c g t t g c c c c c g t g t g g a g g g a a a c c g g g t c g t c a a c c c c a c g a c g g t

600 g g c c t g g t g g a g g t a c t t g g t a a a c a t t t g t t a t g g a a a a g c a g c a g g a g g g t t c t a a g g c c g g a c c a c c t c c a t g a a c c a t t t g t a a a c a a t a c c t t t t c g t c g t c c t c c c a a g a t t c c

660 c t g g g a t t c t g g a g c c c a g g a a c a g g c c a g g c a t g g c t g g a g c g g g t c c g a g t c c g t g c a g a c c c t a a g a c c t c g g g t c c t t g t c c g g t c c g t a c c g a c c t c g c c c a g g c t c a g g c a c g t

720 c t g t g t c c t g t a t g t t g c t c t t a g c c g c a c t c a g g a g c a g a t g t g c a g g t c a c t g c c t a g g a c a c a g g a c a t a c a a c g a g a a t c g g c g t g a g t c c t c g t c t a c a c g t c c a g t g a c g g a t c

780 a a g c t g g t g g g c a c c t c c g t g c a g c a g g c t c t g g g g g g t a t a g c c a c c a t c t g g g c a t g t t t c g a c c a c c c g t g g a g g c a c g t c g t c c g a g a c c c c c c a t a t c g g t g g t a g a c c c g t a c a

840 g t a c a g a g g g c c t t g c t a g t g c g a c a t a t g a g g a a c c a g a a g a t g t g t g g g t t t t g a t t a c a t g t c t c c c g g a a c g a t c a c g c t g t a t a c t c c t t g g t c t t c t a c a c a c c c a a a a c t a a t

900 a a t g g a g t t t t a a t t a a a g t g g c c a g g t t g g g g g a g g t g a g g a g g a a g g c a g g a a g c a g c t t a c c t c a a a a t t a a t t t c a c c g g t c c a a c c c c c t c c a c t c c t c c t t c c g t c c t t c g t c g

960 a g c a g t t t t g a g t c t g a c c g c g t g g a a t c c t a t a a g g t g g t t c t t t c a a g a g g c a t t a t g t c g t c a a a a c t c a g a c t g g c g c a c c t t a g g a t a t t c c a c c a a g a a a g t t c t c c g t a a t a c

1020 c a c a t t c c g c a g c a t g t g t c c c c t g t c t g t a t g g g g c a g c a t c t g c c c t c t g a t c t g t c c g t g t a a g g c g t c g t a c a c a g g g g a c a g a c a t a c c c c g t c g t a g a c g g g a g a c t a g a c a g g

1080 c c a a c a a g g g g t a a t c a c a g a g a a g t a t t a c a t t a t t a a t a t t t a a t t t c c c c t c c t g g t g g t t g t t c c c c a t t a g t g t c t c t t c a t a a t g t a a t a a t t a t a a a t t a a a g g g g a g g a c c a

1140 MTQGVIRCTEPTERDRK t a g c a a c a g a t g a c t c a a g g a g t a a t t a g a t g c a c a g a g c c a a c a g a g a g g g a t a g g a a a a t c g t t g t c t a c t g a g t t c c t c a t t a a t c t a c g t g t c t c g g t t g t c t c t c c c t a t c c t t t

1200 LPDPGPAPSPGPLTFLSLWE t t g c c a g a t c c t g g c c c c g c c c c c t c c c c g g g g c c t c t g a c c t t c c t g t c a c t c t g g g a g a a c g g t c t a g g a c c g g g g c g g g g g a g g g g c c c c g g a g a c t g g a a g g a c a g t g a g a c c c t c

1260 ASEGGELEAQEPSCCCALCA g c t t c t g a g g g a g g g g a g c t g g a g g c c c a g g a g c c t t c c t g c t g c t g t g c t c t c t g t g c g c g a a g a c t c c c t c c c c t c g a c c t c c g g g t c c t c g g a a g g a c g a c g a c a c g a g a g a c a c g c

1320 HGRSPTPPSSALGEAERGRF c a c g g c c g c a g c c c t a c a c c a c c t t c c a g t g c t c t t g g t g a g g c t g a g a g a g g t c g c t t c g t g c c g g c g t c g g g a t g t g g t g g a a g g t c a c g a g a a c c a c t c c g a c t c t c t c c a g c g a a g

1380 VPLGLSFLICKMGFLGPARS g t g c c t t t g g g c c t c a g t t t c c t c a t c t g t a a a a t g g g c t t c c t g g g a c c a g c a a g a t c a c a c g g a a a c c c g g a g t c a a a g g a g t a g a c a t t t t a c c c g a a g g a c c c t g g t c g t t c t a g t

1440 QMTVISQAPKQAAQAVSEGP c a g a t g a c a g t c a t t t c c c a a g c t c c t a a g c a g g c t g c a c a a g c t g t g t c t g a g g g t c c a g t c t a c t g t c a g t a a a g g g t t c g a g g a t t c g t c c g a c g t g t t c g a c a c a g a c t c c c a g g t

1500 VQCLASRQVPPGTRKVWQLS g t g c a g t g c c t g g c c t c t a g g c a g g t g c c c c c t g g g a c g c g t a a g g t t t g g c a g c t c a g c c a c g t c a c g g a c c g g a g a t c c g t c c a c g g g g g a c c c t g c g c a t t c c a a a c c g t c g a g t c g

1560 SDWHRGQLTLCCSPVTVHDT a g t g a c t g g c a t c g t g g c c a g c t g a c t c t g t g t t g c t c c c c a g t t a c g g t c c a c g a c a c a t c a c t g a c c g t a g c a c c g g t c g a c t g a g a c a c a a c g a g g g g t c a a t g c c a g g t g c t g t g t

1620 LVA* t t a g t a g c t t g a a a c t g g g c a c a a g g g g a g a a t t t a c a c c a c a g a a a t c a g g c a a a g c c t a a t c a t c g a a c t t t g a c c c g t g t t c c c c t c t t a a a t g t g g t g t c t t t a g t c c g t t t c g g a

1680 g t a a g t c a g g g t g c c c c c g g c c a g g a g c t g a t t t g c c a g c c t a c g g c t g g g t a g c t c c c a c a t t c a g t c c c a c g g g g g c c g g t c c t c g a c t a a a c g g t c g g a t g c c g a c c c a t c g a g g g t

1740 a c c t t t c c c c a a t g a a t g c a c t c c t c t t c c c c t g t c t g c c c c a a a g g t g c c t c t g a c t c c t g g a a a g g g g t t a c t t a c g t g a g g a g a a g g g g a c a g a c g g g g t t t c c a c g g a g a c t g a g g

1800 a c t g a t c t g c t t t g c t c a g g g c c g a g a a a g g c a c a g c t g a g a g t g g a t t c t g g c t t g g c a t g a c t a g a c g a a a c g a g t c c c g g c t c t t t c c g t g t c g a c t c t c a c c t a a g a c c g a a c c g t

1860 g g g g c c c a g g t t g c c t g t g g g t c c g g g c a a g c c t c a c c c g g g a t g t g c t g g c a g c a g c t g c c c c g g g t c c a a c g g a c a c c c a g g c c c g t t c g g a g t g g g c c c t a c a c g a c c g t c g t c g a c

SbfI 1920 g g a g a g c c t g c a g g a g t g c t t a a g g a g g g g c t g a g g g a g a g g a c g g g c a g c g a g g t g g c c c c t c t c g g a c g t c c t c a c g a a t t c c t c c c c g a c t c c c t c t c c t g c c c g t c g c t c c a c c g g

1980 t g t g t g g a c c c t g c t g g c a g g a g c c g t g g g t g t c t t t a g c c c c g t g t g a g g c c g g c c t g g a c a c a c c t g g g a c g a c c g t c c t c g g c a c c c a c a g a a a t c g g g g c a c a c t c c g g c c g g a c c

2040 g c a g a g g g g t c t g t t g c t g t c a c t g a g g c t g g t g g g c a g c a g g a a g g t g g c t t c a g t a t a c g t c t c c c c a g a c a a c g a c a g t g a c t c c g a c c a c c c g t c g t c c t t c c a c c g a a g t c a t a t

KflI 2100 g t c a a g a a g g t t a t c g g t a t c t a g t g c t g t t t a g c a t g g g g a c c c a c a g c a c a g a a g g t g c a g t t c t t c c a a t a g c c a t a g a t c a c g a c a a a t c g t a c c c c t g g g t g t c g t g t c t t c c a c

2160 g g g a g a c c c t g a g a a c g g g g g t a c a c a g g c a g g t a c c c a g g g c t g c c a g g g a a g c c t t g c c c c t c t g g g a c t c t t g c c c c c a t g t g t c c g t c c a t g g g t c c c g a c g g t c c c t t c g g a a c g

2220 c a a g c a g a g g c c c t g g t c a g g c t t c t c c c a a a c t g g g c a t c c c a g c t g g c a t g g c a c c a g g t t c g t c t c c g g g a c c a g t c c g a a g a g g g t t t g a c c c g t a g g g t c g a c c g t a c c g t g g t c

2280 t c c a g c c c g t g a g g c a g g c g c c g a t g a t t t g a c c c c a c c a g c a g g g c t c t g g g t g a t g g g a g g t c g g g c a c t c c g t c c g c g g c t a c t a a a c t g g g g t g g t c g t c c c g a g a c c c a c t a c c c

2340 a c t g g g g a g t c c t c c t t c a c t t t g g c a g a c g t g t t a t g t t c c a c c t t g t t t g c c c c c a g c t g a c c c c t c a g g a g g a a g t g a a a c c g t c t g c a c a a t a c a a g g t g g a a c a a a c g g g g g t c g

2400 a a g g c c c t t g a a t c t g g a g c g t g g t c t g g g g c t g c a t c a g c c t t c t a g c c t g g g a t g g t c t t c c g g g a a c t t a g a c c t c g c a c c a g a c c c c g a c g t a g t c g g a a g a t c g g a c c c t a c c a g

2460 a g a t t g g c a g a a g g c t g t g g c a t c c t t c a a t g c a g a t g c c c t a g a g a g g g c c g t g g t g g g t c t a a c c g t c t t c c g a c a c c g t a g g a a g t t a c g t c t a c g g g a t c t c t c c c g g c a c c a c c c

2520 a g g c g g g a t c c c t g g g t a g a g g c g g c t c c t c a g t g g c c c a c t g g g c c t c a c t g g a c a g t c t c c g c c c t a g g g a c c c a t c t c c g c c g a g g a g t c a c c g g g t g a c c c g g a g t g a c c t g t c a g

2580 g c c t g t t g c t g c t g c t g g t g g t g c t g g g c c t g g g a g t c c a c c t t g g a g c c a c a g g c c a g g c g g a c a a c g a c g a c g a c c a c c a c g a c c c g g a c c c t c a g g t g g a a c c t c g g t g t c c g g t c c

2640 c a c c t g g g t g g g a g g c a g c c c g g g g c c a g c a c c a g c t c c a c t t g t a c c t g t t g g g c a g g g g t g g a c c c a c c c t c c g t c g g g c c c c g g t c g t g g t c g a g g t g a a c a t g g a c a a c c c g t c c c

2700 * g c t a t c g t g a g t c a g g t g g g c c c t g g t g a g t a c t g c t c a g g g a g a g a g g c c a a g a g c a t c c g a t a g c a c t c a g t c c a c c c g g g a c c a c t c a t g a c g a g t c c c t c t c t c c g g t t c t c g t a g

2760 PYSHEKLTALSPALLAPRTC a g g g a t a a c t g t g t t c c t t g a g t g t g g c c a g g g a t g g t g c c a g g a g a g c t g g t c t g g t g c t c c c t a t t g a c a c a a g g a a c t c a c a c c g g t c c c t a c c a c g g t c c t c t c g a c c a g a c c a c g

2820 GQWRCVKHIAAQCTSWPFCP a t c c c t g c c a a c g a c a c a c t t t g t g a a t g g c t g c c t g g c a a g t g c t c c a t g g a a a g c a g g t a g g g a c g g t t g c t g t g t g a a a c a c t t a c c g a c g g a c c g t t c a c g a g g t a c c t t t c g t c c

2880 SPLVRGEGAAAPGGNSCPKQ g g c t t g g g a g g a c t c g g c c c t c t c c a g c t g c t g c a g g c c c t c c g t t g c t g c a g g g t t t c t c c g a a c c c t c c t g a g c c g g g a g a g g t c g a c g a c g t c c g g g a g g c a a c g a c g t c c c a a a g a

2940 SQERRGGGAETDISWEWAGL g g c t c t g c t c t c t c c t t c c t c c t c c t g c t t c t g t g t c a a t g c t c c a t t c c c a g g c t c c t a c c g a g a c g a g a g a g g a a g g a g g a g g a c g a a g a c a c a g t t a c g a g g t a a g g g t c c g a g g a t

3000 LPLLAQQTCSFRWDWTHSCG g t a g g g g c a a a a g g g c c t g c t g g g t g c a g g a g a a c c t c c a g t c c c a g g t a t g g c t g c a g c c a t c c c c g t t t t c c c g g a c g a c c c a c g t c c t c t t g g a g g t c a g g g t c c a t a c c g a c g t c g

3060 TGIRNWGSSTGVANSNSPFN c c g t g c c a a t c c t g t t c c a g c c a c t t g a g g t g c c g a c a g c a t t g g a g t t g g a g g g a a a g t g g c a c g g t t a g g a c a a g g t c g g t g a a c t c c a c g g c t g t c g t a a c c t c a a c c t c c c t t t c a

3120 PDRHKLETVHVIPYLPAQGK t g g g g t c t c t g t g t t t g a g c t c t g t c a c a t g g a c a a t g g g g t a g a g g g g a g c c t g g c c c t a c c c c a g a g a c a c a a a c t c g a g a c a g t g t a c c t g t t a c c c c a t c t c c c c t c g g a c c g g g a

3180 SCPEPGPTVEEGPQWDQEM t t g a a c a g g g c t c a g g a c c a g g a g t g a c t t c c t c t c c a g g t t g c c a g t c c t g c t c c a t c c a a c t t g t c c c g a g t c c t g g t c c t c a c t g a a g g a g a g g t c c a a c g g t c a g g a c g a g g t a g g

PmlI 3240 c t c a t g t c c t g g a a g a g a g a g a g a g a c g a g g t g g c t g c c c g a g t c c a c g t g a a a g g c a g c g a g t a c a g g a c c t t c t c t c t c t c t c t g c t c c a c c g a c g g g c t c a g g t g c a c t t t c c g t c g

3300 c t c t g a c c t c t t g t c c c c a g g c a g g t g g t g g c g g c t g g c a a c a c a c t c a t a g g g c c c c a t g a g a c t g g a g a a c a g g g g t c c g t c c a c c a c c g c c g a c c g t t g t g t g a g t a t c c c g g g g t a

3360 g a g g g t t c a g g a t t g g a g g g g g t a g a t t t g g g g g c c t c t t a g c t c t c a a a g a g g g g c a a t c t c c c a a g t c c t a a c c t c c c c c a t c t a a a c c c c c g g a g a a t c g a g a g t t t c t c c c c g t t a

3420 t t a a c g g g c a g a g g t c c a t t t g g a t c c a g a c c a t t c a c a g g c t c g a a g g g g a c a c t g g g a a a t t g c c c g t c t c c a g g t a a a c c t a g g t c t g g t a a g t g t c c g a g c t t c c c c t g t g a c c c t

3480 g t t g g g g c t g g g c t c a g g c c t g c t g g g g a a c c a a a a c t t c t g c t t c t g a g g g g t g t c a g t c a a c c c c g a c c c g a g t c c g g a c g a c c c c t t g g t t t t g a a g a c g a a g a c t c c c c a c a g t c a

3540 a a t t c c a t t t t g a c t c t g g g g g a g c c a t t t t a a a t c t g t t t a t t t c c t t c t c a t a g a a t c t t a a g g t a a a a c t g a g a c c c c c t c g g t a a a a t t t a g a c a a a t a a a g g a a g a g t a t c t t a g

3600 a t g g g t g a g a g c t g g c a t g g c c c c t a g a g g t c a t t t g g g g t c c a g c t g c c t c a c c g t a t c t a c c c a c t c t c g a c c g t a c c g g g g a t c t c c a g t a a a c c c c a g g t c g a c g g a g t g g c a t a g

3660 a a t g a g g a a a c t g a g g c c c a g a a a a g a a a a g c a t t t t t g c c c a g a g t c c c t c a g t g a g t c t t a c t c c t t t g a c t c c g g g t c t t t t c t t t t c g t a a a a a c g g g t c t c a g g g a g t c a c t c a g

3720 c t g g t t c c g t a c c t g c t t t c t g c c a g g g a c c a a a c t c c c g t t a g t c a t t c c t g g t g t c a g g a c c a a g g c a t g g a c g a a a g a c g g t c c c t g g t t t g a g g g c a a t c a g t a a g g a c c a c a g t c

3780 c c a g g c a g a g g a g c a g g t g g g g g a g a g g g c g t g g g a g g a g g g a g g t g a g g c a g c t c c c t c g g t c c g t c t c c t c g t c c a c c c c c t c t c c c g c a c c c t c c t c c c t c c a c t c c g t c g a g g g a g

3840 c g a g t g g a a g c t c a c a g t c a g c t g c a g c g c c g c c c c t g c c c t g a c t g c a g c t g a c t g g c a g c t c a c c t t c g a g t g t c a g t c g a c g t c g c g g c g g g g a c g g g a c t g a c g t c g a c t g a c c g t

3900 MRGLTLCGS g g g g c a c c t g g a g g c a g c g g g t t c g g g c g c a g t a t g a g a g g g c t t a c c c t c t g c g g c a g t c c c c g t g g a c c t c c g t c g c c c a a g c c c g c g t c a t a c t c t c c c g a a t g g g a g a c g c c g t c a

3960 GRQDQAAPHILPHPSSRGLW g g g a g a c a g g a c c a g g c a g c t c c c c a c a t c c t t c c a c a t c c g a g c t c c a g g g g t c t c t g g c c c t c t g t c c t g g t c c g t c g a g g g g t g t a g g a a g g t g t a g g c t c g a g g t c c c c a g a g a c c

4020 LVLSQGGRAEVPIPKFLFFK c t t g t c c t t t c a c a g g g c g g g c g c g c c g a a g t t c c t a t t c c g a a g t t c c t a t t c t t c a a a g a a c a g g a a a g t g t c c c g c c c g c g c g g c t t c a a g g a t a a g g c t t c a a g g a t a a g a a g t t t

4080 1 M 1a V 5 10 SKGEELFTGVV EGFP 1 M 1a V 5 10 SKGEELFTGVV EGFP Kozak sequence RYRNFRHHGEQGRGAVHRGG MVSKGEELFTGVV a g g t a t a g g a a c t t c c g c c a c c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t t c c a t a t c c t t g a a g g c g g t g g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a

4140 15 20 25 30 PILVELDGDVNGHKFSVSGE EGFP 15 20 25 30 PILVELDGDVNGHKFSVSGE EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N AHPGRAGRRRKRPQVQRVRR PILVELDGDVNGHKFSVSGE g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t

4200 35 40 45 50 GEGDATYGKLTLKFICTTGK EGFP 35 40 45 50 GEGDATYGKLTLKFICTTGK EGFP GRGRCHLRQADPEVHLHHRQ GEGDATYGKLTLKFICTTGK g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t

4260 55 60 65 70 LPVPWPTLVTTLTYGVQCFS EGFP 55 60 65 70 LPVPWPTLVTTLTYGVQCFS EGFP AARALAHPRDHPDLRRAVLQ LPVPWPTLVTTLTYGVQCFS g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c

4320 75 80 85 90 RYPDHMKQHDFFKSAMPEGY EGFP 75 80 85 90 RYPDHMKQHDFFKSAMPEGY EGFP PLPRPHEAARLLQVRHARRL RYPDHMKQHDFFKSAMPEGY c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t

4380 95 100 105 110 VQERTIFFKDDGNYKTRAEV EGFP 95 100 105 110 VQERTIFFKDDGNYKTRAEV EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R RPGAHHLLQGRRQLQDPRRG VQERTIFFKDDGNYKTRAEV c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a

4440 115 120 125 130 KFEGDTLVNRIELKGIDFKE EGFP 115 120 125 130 KFEGDTLVNRIELKGIDFKE EGFP EVRGRHPGEPHRAEGHRLQG KFEGDTLVNRIELKGIDFKE g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t

4500 135 140 145 150 DGNILGHKLEYNYNSHNVYI EGFP 135 140 145 150 DGNILGHKLEYNYNSHNVYI EGFP GRQHPGAQAGVQLQQPQRLY DGNILGHKLEYNYNSHNVYI g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a

4560 155 160 165 170 MADKQKNGIKVNFKIRHNIE EGFP 155 160 165 170 MADKQKNGIKVNFKIRHNIE EGFP HGRQAEERHQGELQDPPQHR MADKQKNGIKVNFKIRHNIE c a t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g t a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t

4620 175 180 185 190 DGSVQLADHYQQNTPIGDGP EGFP 175 180 185 190 DGSVQLADHYQQNTPIGDGP EGFP GRQRAARRPLPAEHPHRRRP DGSVQLADHYQQNTPIGDGP g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g

4680 195 200 205 210 VLLPDNHYLSTQSALSKDPN EGFP 195 200 205 210 VLLPDNHYLSTQSALSKDPN EGFP RAAARQPLPEHPVRPEQRPQ VLLPDNHYLSTQSALSKDPN c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t

4740 215 220 225 230 EKRDHMVLLEFVTAAGITLG EGFP 215 220 225 230 EKRDHMVLLEFVTAAGITLG EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C REARSHGPAGVRDRRRDHSR EKRDHMVLLEFVTAAGITLG c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c

SrfI 4800 235 MDELYK LFLN 1 5 CCPGCC MEPD EGFP tetracysteine tag 235 MDELYK (in frame with EGFP) LFLNCCPGCCMEPD EGFP HGRAVQVISELLPGLLHGTG MDELYKLFLNCCPGCCMEPD c a t g g a c g a g c t g t a c a a g t t a t t t c t g a a c t g c t g c c c g g g c t g c t g c a t g g a a c c g g a g t a c c t g c t c g a c a t g t t c a a t a a a g a c t t g a c g a c g g g c c c g a c g a c g t a c c t t g g c c t

4860 130 PKKKRKV D 130 PKKKRKV D PKKK SV40 NLS SV40 NLS SV40 NLS (in frame with tetracysteine tag) PKKKRKVDPKKKRKVDPKKK SKKEEKGRSKKEEKGRSKKE PKKKRKVDPKKKRKVDPKKK t c c a a a a a a g a a g a g a a a g g t a g a t c c a a a a a a g a a g a g a a a g g t a g a t c c a a a a a a g a a a g g t t t t t t c t t c t c t t t c c a t c t a g g t t t t t t c t t c t c t t t c c a t c t a g g t t t t t t c t t

4920 RKVI* FRT 130 RKV I* SV40 NLS EKGNIRSSYSEVPIL* RKVI* g a g a a a g g t a a t a t a a g a a g t t c c t a t t c c g a a g t t c c t a t t c t c t a g a a a g t a t a g g a a c t c t t t c c a t t a t a t t c t t c a a g g a t a a g g c t t c a a g g a t a a g a g a t c t t t c a t a t c c t t

4980 FRT c t t c a g c g g c c g c g a c t c t a g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g a a g t c g c c g g c g c t g a g a t c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a

5040 g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a

5100 SV40 poly(A) signal C G T T A T C G T A G T G T T T A SV40pA-R g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a

5160 SV40 poly(A) signal A A G SV40pA-R t t c a c a a a t a a a g c a t t t t t t t c a c t g c g a a t t c t c g t a a a a c t c t t c a t g g c a g t a g t t a a g t g t t t a t t t c g t a a a a a a a g t g a c g c t t a a g a g c a t t t t g a g a a g t a c c g t c a t c a a

5220 a t t a t t c t c t c t c t c t c t c t t t t t c t t t t t t c t t g a g a c a g g a t a t t t c t c t g t t g c c c a t a a t a a g a g a g a g a g a g a g a a a a a g a a a a a a g a a c t c t g t c c t a t a a a g a g a c a a c g g g t

5280 g g c t g g a g t g c a g t g g c a c a g t c t t g g c t c a c t g c a g c c t g g a c c t c c t g g g c t c a a g c c c c g a c c t c a c g t c a c c g t g t c a g a a c c g a g t g a c g t c g g a c c t g g a g g a c c c g a g t t c g g

5340 a t c c t c c c a c c t c a g c c t c c c a a g t a g c t g g g g c t a c a g g c a c a t g g c c a c c a g g c c c a g t a g g a g g g t g g a g t c g g a g g g t t c a t c g a c c c c g a t g t c c g t g t a c c g g t g g t c c g g g t c

5400 a t a a t t t t t c a t t t t t g t a g a g a c t g a g t c t c a c c a t g t t a c c c a g g t t t a t t a t t c t c a t a t t a a a a a g t a a a a a c a t c t c t g a c t c a g a g t g g t a c a a t g g g t c c a a a t a a t a a g a g t

PshAI 5460 t t t t t t a g a t g a a g a g a c t g a g g t c c a g a g a a g c t c a a t g a c t t g c c t a g t t t t a c a a a t a a a a a a t c t a c t t c t c t g a c t c c a g g t c t c t t c g a g t t a c t g a a c g g a t c a a a a t g t t t a

5520 c t c c t g c c a t c a c a t a c c c c t c a g c g t c c t t a a t a a g a g g g a g g c c a c c a a c t a t g t g c t g a g g a c g g t a g t g t a t g g g g a g t c g c a g g a a t t a t t c t c c c t c c g g t g g t t g a t a c a c g a

5580 g g g c a c t g t g g t g g a t g c t g g a g c t a t a g g g t t g a g t a t a t a a g a a a t g g t g t t g c t g g a c c c g t g a c a c c a c c t a c g a c c t c g a t a t c c c a a c t c a t a t a t t c t t t a c c a c a a c g a c c t

5640 g c a a c t g t t g c t t g c t t a c c t g a c c t a t c t g a g a a t t a a t t a g c a g g g g a a c a t a t t t t t c g t t g a c a a c g a a c g a a t g g a c t g g a t a g a c t c t t a a t t a a t c g t c c c c t t g t a t a a a a a

5700 g t t t t c a g a t t c a a t a t a a g a a c t t g t g t g g g c a a a a a t a a a g a t c a g t a g t a a t a a c a g c a a a a g t c t a a g t t a t a t t c t t g a a c a c a c c c g t t t t t a t t t c t a g t c a t c a t t a t t g t c

5760 t a g t t c c c a t t t g c t g a c t g t a c t g t c c t a a g t g c a t a t a t a t a t a c a t a c a c a c a c g c a a t c a a g g g t a a a c g a c t g a c a t g a c a g g a t t c a c g t a t a t a t a t a t g t a t g t g t g t g c g t

5820 t a c c t a t a c t c c t c t a a t a c t c a a a a t g a t c c t g t t t a t g t a t t g t t a a t a t g c t c a t t t a t g g a t a t g a g g a g a t t a t g a g t t t t a c t a g g a c a a a t a c a t a a c a a t t a t a c g a g t a a a

5880 t a t t t t t a a a t t t t t a t t t a t t t t t a t t t t t a t t t a t t t t t g a g a c g g a g t c t c a t t c t g a t a a a a a t t t a a a a a t a a a t a a a a a t a a a a a t a a a t a a a a a c t c t g c c t c a g a g t a a g a c

5940 t c g c g g a g g c t g a a g t g c a g t g g t g c g a t c t c a g c t c a g t g c g a c c t c c g c c t c c c g g g t a g c g c c t c c g a c t t c a c g t c a c c a c g c t a g a g t c g a g t c a c g c t g g a g g c g g a g g g c c c a

6000 t c a a g t g a t t c t c c t g c c t c a g c c t c c g g a c t a g c t g g g a t t a c a g g c g c c c g c c t c c a c a g t t c a c t a a g a g g a c g g a g t c g g a g g c c t g a t c g a c c c t a a t g t c c g c g g g c g g a g g t g

6060 g c c c a g c t a a t t t t t g t a t t t t t a g t a g a g a t g g g g t t t c g c c a t g t t g g c c a g g c t g g t c g g g t c g a t t a a a a a c a t a a a a a t c a t c t c t a c c c c a a a g c g g t a c a a c c g g t c c g a c c a

6120 c t c g t a c t c c t g a c c t t g a g t g a t c c a c c t g c c t c g g c c t c c c a a a g t g c t g g g a t t a c a g a g c a t g a g g a c t g g a a c t c a c t a g g t g g a c g g a g c c g g a g g g t t t c a c g a c c c t a a t g t

6180 g g c a t g a g c c a c c g c g c c g g g c t a a t a t g c t c a t t t t a g t g a g g c a a a a a t a g a g g c t c a c c g t a c t c g g t g g c g c g g c c c g a t t a t a c g a g t a a a a t c a c t c c g t t t t t a t c t c c g a g t

6240 g a g t c t g a t t t g t a c a a a a c t a c a g a g c a g t t a a g t g t c c t c t c a g a t g t g t a c c c t g a t c t c a g a c t a a a c a t g t t t t g a t g t c t c g t c a a t t c a c a g g a g a g t c t a c a c a t g g g a c t a

6300 c t g g g t g a c t c t a g g a c t c t a g g t c t c a a c t g t t a c a a c c a g t t a a g g g t t t g g g g a a g c g a c c c a c t g a g a t c c t g a g a t c c a g a g t t g a c a a t g t t g g t c a a t t c c c a a a c c c c t t c g

ZraI 6360 a c t g g g c c a a g a g t c a g g a a a a t g g a a g c c a c a g g t a g t g c a a g g t c t t g g g a a t g g g a c t g a c c c g g t t c t c a g t c c t t t t a c c t t c g g t g t c c a t c a c g t t c c a g a a c c c t t a c c c t g

AatII 6420 g t c t g g t c c a a g g a t t c a c g c g a t g a c t g g a a c c c g a a g a g c c g g g g c c c g g t t t a c g g c c a g a c c a g g t t c c t a a g t g c g c t a c t g a c c t t g g g c t t c t c g g c c c c g g g c c a a a t g c c g

6480 c g c c a t g a a g c a a c g c g c g c c g g t a g g t t t g g g a a t c a g g g a g c c c t c t g a a t a g g a g a c g c g g t a c t t c g t t g c g c g c g g c c a t c c a a a c c c t t a g t c c c t c g g g a g a c t t a t c c t c t g

6540 t g a g t t g g g a g g g a a a g g g g c t t c g c t g g g g g a g c c t c g g c t t c t t c t g g g a g a a a a t t c a c t c a a c c c t c c c t t t c c c c g a a g c g a c c c c c t c g g a g c c g a a g a a g a c c c t c t t t t a a g

SgrAIMreI 6600 c c a c g g c t a c c t a g t g a g c c t g c a a a c t g g t a g g c g c c g g c g t a g g c g c g c g g g c g g g g c g g t g c c g a t g g a t c a c t c g g a c g t t t g a c c a t c c g c g g c c g c a t c c g c g c g c c c g c c c c g

6660 c g g g g g c g g g g c c t g c g g g g c g t g g c g g g g c g g g c a g a g g g c g g g g c c t g c t t c t c c t c a g c c c c c g c c c c g g a c g c c c c g c a c c g c c c c g c c c g t c t c c c g c c c c g g a c g a a g a g g a g t

6720 g c t t c a g g c g g c t g c g a c g a g c c c t c a g g c g a a c c t c t c g g c t t t c c c g c g c g g c g c c g c c g a a g t c c g c c g a c g c t g c t c g g g a g t c c g c t t g g a g a g c c g a a a g g g c g c g c c g c g g c g

6780 c t c t t g c t g c g c c t c c g c c t c c t c c t c t g c t c c g c c a c c g g c t t c c t c c t c c t g a g c a g t g a g a a c g a c g c g g a g g c g g a g g a g g a g a c g a g g c g g t g g c c g a a g g a g g a g g a c t c g t c a

6840 c a g c c c g c g c g c c g g c c g g c t c c g t t a t g g c g a c c c g c a g c c c t g g c g t c g t g g t g a g c a g t c g g g c g c g c g g c c g g c c g a g g c a a t a c c g c t g g g c g t c g g g a c c g c a g c a c c a c t c g t

6900 g c t c g g c c t g c c g g c c c t g g c c g g t t c a g g c c c a c g c g g c a g g t g g c g g c c g g g c c c t g a c g a g c c g g a c g g c c g g g a c c g g c c a a g t c c g g g t g c g c c g t c c a c c g c c g g c c c g g g a c t

BspDI*ClaI* 6960 g g c g c g g g a t c g a t c c a t g a c a c c c c c c c g c c c c c c a t a t c t c a c t a t a a t t c a c t g t c a c c g c g c c c t a g c t a g g t a c t g t g g g g g g g c g g g g g g t a t a g a g t g a t a t t a a g t g a c a g t

7020 a a t g g c t t t t a t g a t t t g g a g t t t g g g g a t a c t t t t a a a g a c a g g g t c t t g g t a g c t c c t t t a c c g a a a a t a c t a a a c c t c a a a c c c c t a t g a a a a t t t c t g t c c c a g a a c c a t c g a g g a

7080 a g a t g g c t t g g g a t t c a c t t a g t a a c c c t t g c t g g c c t a a a a t t c a g g a a c t t c t a c c t c t c t a c c g a a c c c t a a g t g a a t c a t t g g g a a c g a c c g g a t t t t a a g t c c t t g a a g a t g g a g

7140 a t c t t c c c a a g t a c t g g g t t a g t a g g g a t g t c c a g c a t a t t t a g c t t g c t t t a a t g a t t t t a g a a g g g t t c a t g a c c c a a t c a t c c c t a c a g g t c g t a t a a a t c g a a c g a a a t t a c t a a a

7200 t g c t g t a c t t t t t t c t g t g t g t a c a g g t g c a t g t a t a t t t a t a a a a c t a a c a t a a t a t a t a c g a c a t g a a a a a a g a c a c a c a t g t c c a c g t a c a t a t a a a t a t t t t g a t t g t a t t a t a t a

7260 t t t a c c c c c a a a g t a c c a t a a c c c t a a t t a a t g c t a t t a a a t g t t t t g t a t g c t g g g c t c a a a t g g g g g t t t c a t g g t a t t g g g a t t a a t t a c g a t a a t t t a c a a a a c a t a c g a c c c g a g

7320 t g g g c a t a c t a a t a t a a c c a a t a t t a t a c a a t g c a a a a c a a t t t t g g a a t a t c t t c t g t a a c c c g t a t g a t t a t a t t g g t t a t a a t a t g t t a c g t t t t g t t a a a a c c t t a t a g a a g a c a t

7380 a a a t t a t c t t a t t a g t a a g t t g t a a c c a t c t c a g a c t t c t a a g t t t g a g t t t g t a a a g g a t t t a a t a g a a t a a t c a t t c a a c a t t g g t a g a g t c t g a a g a t t c a a a c t c a a a c a t t t c c t

BstEII 7440 t c a g t t t t t t t t g g t g a c c t a c a c a c a c a c a c a c a c a c a c a c a c a c a c a c a c a c a c a c a g a g t c a a a a a a a a c c a c t g g a t g t g t g t g t g t g t g t g t g t g t g t g t g t g t g t g t g t g t g t c

7500 g g g g g t g g a t t c t g a g a a t t t a g a g a a t t t a g a t g g t t a a t a c c a g t t t t t a t t t t t a t t c c c c c a c c t a a g a c t c t t a a a t c t c t t a a a t c t a c c a a t t a t g g t c a a a a a t a a a a a t a a

7560 c t g t g t g a t a t t t a t t t a t t a a g a t t t a t t t a t t t a t t a c a t g t a g g t a c a c t g t a t c t g g a c a c a c t a t a a a t a a a t a a t t c t a a a t a a a t a a a t a a t g t a c a t c c a t g t g a c a t a g a c

7620 t c t t c a g a c a c a c c a g a a g a g g g c a t c a g a t c t c g t t a c a g a t g g t t g t g a g t c a c c a t g a g a a g t c t g t g t g g t c t t c t c c c g t a g t c t a g a g c a a t g t c t a c c a a c a c t c a g t g g t a c

7680 t g g t t g c t g g g a t t t g a a c t c a g g a c c t c t g g a a g a a c a g t c a g t g c t c t t a a c c g t t g a a c c a a c g a c c c t a a a c t t g a g t c c t g g a g a c c t t c t t g t c a g t c a c g a g a a t t g g c a a c t

7740 g c c a t c t c a c c a g c c c c g t g a t a t t t t a t t t a a c t t a c t a g a a t a t a c t g t g t t a a a a c a c g g t a g a g t g g t c g g g g c a c t a t a a a a t a a a t t g a a t g a t c t t a t a t g a c a c a a t t t t g t

7800 t a t t t t a g g g g a t t g g g g a g a t g g c c c a t t c t g t a a a g t g t c t g c c a t g t c a t c t t c a g g a t a a a a t c c c c t a a c c c c t c t a c c g g g t a a g a c a t t t c a c a g a c g g t a c a g t a g a a g t c c

7860 a c c t g c g t t t g t a a c c c c a g c a t g g a g a a g a c a g a g a c a g g g a g a c c c c t g g g c t t c c t g t g g a c g c a a a c a t t g g g g t c g t a c c t c t t c t g t c t c t g t c c c t c t g g g g a c c c g a a g g a c

7920 g c a a g c t a g a c t a g c a a a t t t g g t t a a c t a g g t t c a a t g a g a g a c t c a g t c t c a a a a c a t c g t t c g a t c t g a t c g t t t a a a c c a a t t g a t c c a a g t t a c t c t c t g a g t c a g a g t t t t g t a

7980 a a g a a g t g a a t t t t t t a g a c c c a a t t t c a a t c t c t g a a c t c c a t a c g t a t g c a c a t g t a c t t c t t c a c t t a a a a a a t c t g g g t t a a a g t t a g a g a c t t g a g g t a t g c a t a c g t g t a c a t g

8040 c t g c a t a t a a a t a t a t a t a c a c a c c c a t a a a c a c a t g c a c a c a c a a a a a a t g g t t c a a a a g a c g t a t a t t t a t a t a t a t g t g t g g g t a t t t g t g t a c g t g t g t g t t t t t t a c c a a g t t t t

8100 g t t c t t a a a a t g t a t a a g a g t a g a a a g t g t a g a g c c g g g a g t g g t g g c g c a c g c c t t t a a c a a g a a t t t t a c a t a t t c t c a t c t t t c a c a t c t c g g c c c t c a c c a c c g c g t g c g g a a a t t

8160 t c c c a g c a c t t g g g a g c a g a g g c a g g t g g a t t t c t g a g t t c g a g g c c a g c c t g g t c t a c a a g g g t c g t g a a c c c t c g t c t c c g t c c a c c t a a a g a c t c a a g c t c c g g t c g g a c c a g a t g t

8220 g a g t g a g t t c c a g g a c a g c c a g a g c t a t a c a g a g a a a t c c t g c c t c a a a a a a c c a a a a a t c t c a c t c a a g g t c c t g t c g g t c t c g a t a t g t c t c t t t a g g a c g g a g t t t t t t g g t t t t t a

8280 a a a g t g t a t a t a c t a a t c t t t c a t g c c t a a a a t a t t t a t t t t t g c c a c a t t t t t c a t a c c t t t c a c a t a t a t g a t t a g a a a g t a c g g a t t t t a t a a a t a a a a a c g g t g t a a a a a g t a t g g

8340 t c t t t a a t a t t t t t c t t t t t c t c t t t g t g c t t c a a a g g a a a t t t t g t g t c a t g t t t t a c t a g a a a t t a t a a a a a g a a a a a g a g a a a c a c g a a g t t t c c t t t a a a a c a c a g t a c a a a a t g a

8400 t t t c a t t c t t g a c t c t a t t c a g c a g t a a g a c g c a g c a t g g c t t t c t t a g a g a a g t a t a a t a a a g t a a g a a c t g a g a t a a g t c g t c a t t c t g c g t c g t a c c g a a a g a a t c t c t t c a t a t t a

8460 c t a t t a c a a a a c g g t a a c a t t t a a c t g c t c t a c a t a c c c t c t t g t a g c t a a a t t c c t c t g g a t a a t g t t t t g c c a t t g t a a a t t g a c g a g a t g t a t g g g a g a a c a t c g a t t t a a g g a g a c

8520 t t a a a c t a a g a c t t a t t a t c t a a a c g t a a t t a a a t g t t g a t a t c t t a t a a t g c t c c a c t t a a t t t g a t t c t g a a t a a t a g a t t t g c a t t a a t t t a c a a c t a t a g a a t a t t a c g a g g t g a a

8580 t g a a a c a g c t g t c t t t c t t t t g c a g a t t a g c g a t g a t g a a c c a g g t t a t g a c c t a g a t t t a c t t t g t c g a c a g a a a g a a a a c g t c t a a t c g c t a c t a c t t g g t c c a a t a c t g g a t c t a a a

8640 g t t t t g t a t a c c t a a t c a t t a t g c c g a g g a t t t g g a a a a a g t g t t t a t t c c t c a t g g a c t c a a a a c a t a t g g a t t a g t a a t a c g g c t c c t a a a c c t t t t t c a c a a a t a a g g a g t a c c t g a

8700 g a t t a t g g a c a g g t t a g t a a g a c c t c g a t t g a a g t t a t t t t t a g a g g g t g t g t g t g t g g t c t a a t a c c t g t c c a a t c a t t c t g g a g c t a a c t t c a a t a a a a a t c t c c c a c a c a c a c a c c a

8760 g t g t g t g t g t g t g t g t g t g t g t g t g t g t g t g t g t g t g t a a g a g a g a g a g g a g a g g c a t g t c a c a c a c a c a c a c a c a c a c a c a c a c a c a c a c a c a c a c a t t c t c t c t c t c c t c t c c g t a c a

8820 g t g a t a t a t a t g t g a a g t g t a t t g c a t g t a c a t g g a a t c c a g a g a a g g a c a t t a g g t g t c c a c t a t a t a t a c a c t t c a c a t a a c g t a c a t g t a c c t t a g g t c t c t t c c t g t a a t c c a c a g

8880 c t a c t c t g t t g c t c t c t t a t t c c c t t g a a t c a g t g t c t c t t g c t g a a c c t g g g g c a t a c a g a t g a g a c a a c g a g a g a a t a a g g g a a c t t a g t c a c a g a g a a c g a c t t g g a c c c c g t a t g t

8940 t a c t g a t t t g g c t a g g c t g c c t g g c t a g c a a g a c c c a g g g a t c t g c t t g t c t c t g c c c c c a t g a c t a a a c c g a t c c g a c g g a c c g a t c g t t c t g g g t c c c t a g a c g a a c a g a g a c g g g g g

9000 c a g t g c t g a c g t t a c a a g c a g g t g c a a c c a c a t c t g g t t t t t a t a t g g g t a c t g g g g g a t g t c a c g a c t g c a a t g t t c g t c c a c g t t g g t g t a g a c c a a a a a t a t a c c c a t g a c c c c c t a

9060 c t g a a c t t a g g g c c t g a t g c g t g c c c a a g t g c t c t c a c c c g c c a a g c c a c c t c t c c a g c c g a c t t g a a t c c c g g a c t a c g c a c g g g t t c a c g a g a g t g g g c g g t t c g g t g g a g a g g t c g g

9120 a a a g g g t t t t t t t t c t t g t a t t a a t t c t a a c t t a g c a a c t g t t g a g t a g t t g a t g t t t g a t t t c c c a a a a a a a a g a a c a t a a t t a a g a t t g a a t c g t t g a c a a c t c a t c a a c t a c a a a c t

9180 a t a t a a g c t a a g c c a a c t t a t t a g a a t a a a t g t t t t c t a t t t t t t a c t t g t a t g t g t g a g t a t a t t c g a t t c g g t t g a a t a a t c t t a t t t a c a a a a g a t a a a a a a t g a a c a t a c a c a c t c

9240 t g c c t g c a t g t a t g t a t g t g a a g c a t a t g t a t t c c a g g a t g t c a g a a t t g t g a t g g a t t c a c g g a c g t a c a t a c a t a c a c t t c g t a t a c a t a a g g t c c t a c a g t c t t a a c a c t a c c t a a g

9300 c c t g g a a c t g g a g t t a c a g a c c a t t g t g a a c c a c t g t g t a t g g g t g c t g g g a a c t g a a c t g g a c c t t g a c c t c a a t g t c t g g t a a c a c t t g g t g a c a c a t a c c c a c g a c c c t t g a c t t g a

9360 t t a g g t c c t c t g c a a a a a c a g t a a g g a t c t t a a c t g a t a a a c t a t c t t t c t a g c c c t a a g a a t c c a g g a g a c g t t t t t g t c a t t c c t a g a a t t g a c t a t t t g a t a g a a a g a t c g g g a t t c

9420 g c t t t c t a c t t t t a a a g t t c g t t t c t c t t t c t c t c t c t c t c t c t c t c t c t c t c t c t c t c t c g a a a g a t g a a a a t t t c a a g c a a a g a g a a a g a g a g a g a g a g a g a g a g a g a g a g a g a g a g a

9480 c t c c c t c t c c c t c c c t c c t c c t c c t c c t c c t c t t t c c c c c c t c t c g c c t c c t c t t t c t c t g a g g g a g a g g g a g g g a g g a g g a g g a g g a g g a g a a a g g g g g g a g a g c g g a g g a g a a a g a g a

9540 c t c c c c t c c c t c t c t c t c t c t c a c a c a c a c a c a c a g a c a g a g a g a g g a g a g a g a a a g g a a g a g g g g a g g g a g a g a g a g a g a g t g t g t g t g t g t g t c t g t c t c t c t c c t c t c t c t t t c c t t

9600 g c c t t a c t a a t t a a t t a c c t a g t t t t a g a c a t a a c t a g t g g g a a t c c a t a g t a a g t a a a t c g g a a t g a t t a a t t a a t g g a t c a a a a t c t g t a t t g a t c a c c c t t a g g t a t c a t t c a t t t a

9660 c a a c a t t c t t g a a g a g a g g t t a g a a g a a t t g g c t c t c g t t g t c c a t c g a g g c t g t a t a a g g t t g t a a g a a c t t c t c t c c a a t c t t c t t a a c c g a g a g c a a c a g g t a g c t c c g a c a t a t t c

9720 a t t a t t c t t c t g t t t t c t c a g c t t a c c a t a c a t a t t t t c t a c g t a g a c a a t a c c a a t t c a t a a t a a g a a g a c a a a a g a g t c g a a t g g t a t g t a t a a a a g a t g c a t c t g t t a t g g t t a a g t

9780 a a t a c a c t g t a c g t a a a g t a t a t g c g c a c a c a c a c a c a c a c a c a c a c a c a c a c a c a c a c a t t a t g t g a c a t g c a t t t c a t a t a c g c g t g t g t g t g t g t g t g t g t g t g t g t g t g t g t g t g t

9840 c a a g t t a c a t a t t g t c a t a t a g t a c t a a a a t t t a t g g a c t t t t t c a t a t t a a t a c a t c t a g t t c a a t g t a t a a c a g t a t a t c a t g a t t t t a a a t a c c t g a a a a a g t a t a a t t a t g t a g a t

9900 g a g c t a t c a t g t t t t t t a t c t t t t a a a a a t t a c t t t a c g g g g c t a c a g a g a t g t c t c a g c c t c g a t a g t a c a a a a a a t a g a a a a t t t t t a a t g a a a t g c c c c g a t g t c t c t a c a g a g t c g

9960 a g t t a g g a g c a c t g a c t g c t c t t c c a g a g g t c c t g a g t t c a a t t c c c a g c a a c c a c a t g g t c a a t c c t c g t g a c t g a c g a g a a g g t c t c c a g g a c t c a a g t t a a g g g t c g t t g g t g t a c c

10,020 t g g c t c a c a a c c a t c t g t a a t g g g a t c t g a t c c a c t c t t a t g g t g t g t c t g a a g a c a g t g a c c g a g t g t t g g t a g a c a t t a c c c t a g a c t a g g t g a g a a t a c c a c a c a g a c t t c t g t c a c

10,080 a c a g t g t a c t c a t a t a c a t g a a a t a a a t c t t t a a a a a a t t a c t t t a c c a t c a g g g t a t a t t g t c a c a t g a g t a t a t g t a c t t t a t t t a g a a a t t t t t t a a t g a a a t g g t a g t c c c a t a t a

10,140 a t a t a t a t a t a t a t g t g t g t g t g t g t g t g t g t g t g t a t g a a g t a t t t g c t a t t t g a g a g g t a t a t a t a t a t a t a c a c a c a c a c a c a c a c a c a c a c a t a c t t c a t a a a c g a t a a a c t c t c c

10,200 g t c t c a c t g t t t a g c t c a g t c t g g c t t g g a a c g t t c t g t a t a g c c c a g a c t g g c c t c a a c c a g a g t g a c a a a t c g a g t c a g a c c g a a c c t t g c a a g a c a t a t c g g g t c t g a c c g g a g t t g

10,260 t c a g c a g t c c c c c t g a a a g g c t c t c a t a t g t g g a g a t t a c a g g t a t g t g c t a c t g t g c c c a g t c g t c a g g g g g a c t t t c c g a g a g t a t a c a c c t c t a a t g t c c a t a c a c g a t g a c a c g g g

10,320 a g c c c t a c a t g t t a c t t t g t t t t t g a a a t a g g g t a t c a a a t a g c t t a g a c t g g c t t c t a a t c g g g a t g t a c a a t g a a a c a a a a a c t t t a t c c c a t a g t t t a t c g a a t c t g a c c g a a g a t t

10,380 g t c a g t g t g t a g c t a a g g a t g g t c t g a g c t t c t g a a c c t g c t a c t t c a a c t c c t g g t g t g c a g t c a c a c a t c g a t t c c t a c c a g a c t c g a a g a c t t g g a c g a t g a a g t t g a g g a c c a c a c

10,440 c c a t c a t g g c c a g t t t g c a c a g t t g t g a g a g t a g a a c t c a g g a c c t c c t a t g t g t t a c t a g g t a g t a c c g g t c a a a c g t g t c a a c a c t c t c a t c t t g a g t c c t g g a g g a t a c a c a a t g a t

10,500 g a c a t g t t c t c t a c c a g c t g a g c t a c a t c c c a a t t c g c c t a c a t t t a c t t t t a t t t t t t a c t g t a c a a g a g a t g g t c g a c t c g a t g t a g g g t t a a g c g g a t g t a a a t g a a a a t a a a a a a t

10,560 a a a g a t t t a t t t a t g t a t a c a g c a c t c t g c c t g c a t g t a c a t c t g c a t g c c a g a a g a g g g t t t c t a a a t a a a t a c a t a t g t c g t g a g a c g g a c g t a c a t g t a g a c g t a c g g t c t t c t c c c

10,620 c a c c a g a t c t c a t c a t a c a t a g t t g t g a g c c a c c a t g t g g t t g c t g g g g a t t g a a c t c a g g t g g t c t a g a g t a g t a t g t a t c a a c a c t c g g t g g t a c a c c a a c g a c c c c t a a c t t g a g t c

10,680 g g c c t c t g g a a g a g c a g t c a g t g c t c t t a a c c t c t g a g c c a t c t c t g t a g c c c c c t a c a t c c g g a g a c c t t c t c g t c a g t c a c g a g a a t t g g a g a c t c g g t a g a g a c a t c g g g g g a t g t a

10,740 t t a t t t t a a a g a t t a t a g g c t a t t c t a t t c a t a g a t g t g c c a t a a c t g a t t t a a c c a g a t a a t a a a a t t t c t a a t a t c c g a t a a g a t a a g t a t c t a c a c g g t a t t g a c t a a a t t g g t c t a

10,800 c t t c a t t a t t t t g t a c t t a g t a g a g t a t a t t a g a g g g a a t g a a t c t t a a a t a c a c a t t t t g a a g t a a t a a a a c a t g a a t c a t c t c a t a t a a t c t c c c t t a c t t a g a a t t t a t g t g t a a a a

10,860 g a c a c t t g c t c a g c t t t a g a c a a g t a a a a a t c a c t g g t c a a g g a a a t g g t g c t t a g t g g a c t g t g a a c g a g t c g a a a t c t g t t c a t t t t t a g t g a c c a g t t c c t t t a c c a c g a a t c a c c t

10,920 t g c t t c t g a a c t g t c a a a t t g t c c t c c a a c a a g t t a c a t c a t t t t a t a c t t c c a a c g t t a a c g a a g a c t t g a c a g t t t a a c a g g a g g t t g t t c a a t g t a g t a a a a t a t g a a g g t t g c a a t

10,980 t g t g a g a g t t c a t t t t t c t t t a g g c t c t t c t a g t a t t t t a t a t t t g a g a a a t a a a a t t t a a c a c t c t c a a g t a a a a a g a a a t c c g a g a a g a t c a t a a a a t a t a a a c t c t t t a t t t t a a a t

11,040 a c c a a a a t t t g c c a a t c t a g t a a g c t t g t t t t t a t t t g t a t c t t t a a a t t t t a t g a t a g a t g g t t t t a a a c g g t t a g a t c a t t c g a a c a a a a a t a a a c a t a g a a a t t t a a a a t a c t a t c t

11,100 t g t t a t a g t g t a c t c t c c t c t c c c c t c c c c t c c c c t c t c c t c c t c a g c a g g a t c t t g t t a a c a a t a t c a c a t g a g a g g a g a g g g g a g g g g a g g g g a g a g g a g g a g t c g t c c t a g a a c a a t

11,160 t g t a g c c c t a a g g g a t c t a a t a c t t a c t c t g t a g t c c a g g c t g t c c t t c a g c t c a t g a g a a c a t c g g g a t t c c c t a g a t t a t g a a t g a g a c a t c a g g t c c g a c a g g a a g t c g a g t a c t c t

11,220 c a c a c c a t c a t g c t t g g c a t g t t t c t t g t t c t g a a a g g t a t c t g g t t t t a a t t t t g g t t g g t g t g g t a g t a c g a a c c g t a c a a a g a a c a a g a c t t t c c a t a g a c c a a a a t t a a a a c c a a c

11,280 a g c t g g t g t g t t g g t g t a t a t c t g t a a t c t c a g c a c a g g c a a g t a g a g a c a a g g a t t a t g t c g a c c a c a c a a c c a c a t a t a g a c a t t a g a g t c g t g t c c g t t c a t c t c t g t t c c t a a t a c

11,340 a t t a c a g t c c a g c c t g g g c t a t t t a g a g g g a t t c t c t t t a a t c t t t c c t c a t g c c c c a a a t a a t g t c a g g t c g g a c c c g a t a a a t c t c c c t a a g a g a a a t t a g a a a g g a g t a c g g g g t t t

11,400 a t c t t a c c t t t g g t a t a t g a a a a a t a g t c t c c a c t t c t g c a a a a t a t t g c t t t a t g a a g t t a g a a t g g a a a c c a t a t a c t t t t t a t c a g a g g t g a a g a c g t t t t a t a a c g a a a t a c t t c a

11,460 a a g a a t t a a t t c c c t t c a t a g a g a c a a g g a a t g t g t c c t g t a a a a g t t t a a t g t g t a a g a t t c t t a a t t a a g g g a a g t a t c t c t g t t c c t t a c a c a g g a c a t t t t c a a a t t a c a c a t t c t

11,520 a g t a t t t g t t a t a a a a g a t a a a t a t t c a g a a t c t t c t t t t t a a t t c c t g a t t t t a t t t c t t c a t a a a c a a t a t t t t c t a t t t a t a a g t c t t a g a a g a a a a a t t a a g g a c t a a a a t a a a g a

11,580 a t a g g a c t g a a a g a c t t g c t c g a g a t g t c a t g a a g g a g a t g g g a g g c c a t c a c a t t g t g g t a t c c t g a c t t t c t g a a c g a g c t c t a c a g t a c t t c c t c t a c c c t c c g g t a g t g t a a c a c c

11,640 c c c t c t g t g t g c t c a a g g g g g g c t a t a a g t t c t t t g c t g a c c t g c t g g a t t a c a t t a a a g g g g a g a c a c a c g a g t t c c c c c c g a t a t t c a a g a a a c g a c t g g a c g a c c t a a t g t a a t t t c

11,700 c a c t g a a t a g a a a t a g t g a t a g a t c c a t t c c t a t g a c t g t a g a t t t t a t c a g a c t g a a g a g t g a c t t a t c t t t a t c a c t a t c t a g g t a a g g a t a c t g a c a t c t a a a a t a g t c t g a c t t c t

11,760 g c t a c t g t g t a a g t a t a a t t a a c t t a t a a t t a a a a a a a t a g g g c c a t t c t a g t t t t a t c t c g a t g a c a c a t t c a t a t t a a t t g a a t a t t a a t t t t t t t a t c c c g g t a a g a t c a a a a t a g a

11,820 a t a t t t t t t t t a a a c t t g t g c a a a c t a t g c t a c a t a a t c a a c t t a c t t g a t t a t g t a a t a t a t a a a a a a a a t t t g a a c a c g t t t g a t a c g a t g t a t t a g t t g a a t g a a c t a a t a c a t t a t

11,880 t a t a t a g t a a t a g t t g g a a t t a c t a t t a t t a c a t a t t t a t t t t t c t g t g c t g g g g t a g a a a t a t a t c a t t a t c a a c c t t a a t g a t a a t a a t g t a t a a a t a a a a a g a c a c g a c c c c a t c t t

11,940 c c c a g g g a a c t a a g t a c t g t a a t a a c c g a g c t t c a t t c a t c c t a g t c a t t t t t a a t t t t t g g g t c c c t t g a t t c a t g a c a t t a t t g g c t c g a a g t a a g t a g g a t c a g t a a a a a t t a a a a a

12,000 t t t t t t t t t t t t g t t t t t t c g a g a c a a g g t t t c t c t g t g t a g c c c t g g c t g t c c t g g a a c a a a a a a a a a a a a c a a a a a a g c t c t g t t c c a a a g a g a c a c a t c g g g a c c g a c a g g a c c t t g

12,060 t c a c t c t g t a g a c c a g g c t g g c c t c g a a c t c a g a a a t c t g c c t g c c t c t g c c t c c c a g t g a g t g a g a c a t c t g g t c c g a c c g g a g c t t g a g t c t t t a g a c g g a c g g a g a c g g a g g g t c a c

12,120 c t g a g a t t a a t g a c g t g c a c c a c c a c t g c c t g g c a t t t t t t t a a a g t t t t a a t t t g a g a c g a c t c t a a t t a c t g c a c g t g g t g g t g a c g g a c c g t a a a a a a a t t t c a a a a t t a a a c t c t g

12,180 c c t t t c t c a c t a a c t t g c t c a c a c t a t c c t c g a a c t c a t t a c g t g g g c t g g t c t a g a a a t g g a a a g a g t g a t t g a a c g a g t g t g a t a g g a g c t t g a g t a a t g c a c c c g a c c a g a t c t t t a

12,240 t t c a g t c a a c t t g c c t c a g c t t c t c a a g t a g c t g g g a t t a c a g g c a g g t g c t a c c a c t t c a a g t c a g t t g a a c g g a g t c g a a g a g t t c a t c g a c c c t a a t g t c c g t c c a c g a t g g t g a a g

12,300 t g g c c a t g a t c a t t g t t g t t g t t g c t a t t a t t a t t a t t a t t a t t t c a c t g a t a c t t c a t a a c c g g t a c t a g t a a c a a c a a c a a c g a t a a t a a t a a t a a t a a t a a a g t g a c t a t g a a g t a t

12,360 t c a c a a c a c a g a a t a g t a a c t g t a g t t c a t g a c c a a c a a a a a a g t t g t a c a g t t t c t c t g a g t g t t g t g t c t t a t c a t t g a c a t c a a g t a c t g g t t g t t t t t t c a a c a t g t c a a a g a g a c

12,420 t t g t c t t a t t t t g a t c t g g a t t t t g a t t t g t t t c a t g g c a c t g g g c a g g c t a a t c t c a a a a a c a g a a t a a a a c t a g a c c t a a a a c t a a a c a a a g t a c c g t g a c c c g t c c g a t t a g a g t t t

12,480 c t g c a t g g c t c a a a t a g t c c t t c t g t c t t a a t c t c c c a t g t t c t g g a a g t a t a g g t a t c a g a c g t a c c g a g t t t a t c a g g a a g a c a g a a t t a g a g g g t a c a a g a c c t t c a t a t c c a t a g t

12,540 c t a c a c c t a g c t g t a t t t t c a t a t t t t a a a a a a a a c g c c t a a g a a t t g c t a t t g a a t g a g g a t g t g g a t c g a c a t a a a a g t a t a a a a t t t t t t t t g c g g a t t c t t a a c g a t a a c t t a c t c

12,600 g c g g a g a g a t g g c t c a g c a g t c a c a a g t a c t t g t a g a g g a c t g g g g t t t g g t t a c t a g c a c g c c t c t c t a c c g a g t c g t c a g t g t t c a t g a a c a t c t c c t g a c c c c a a a c c a a t g a t c g t

12,660 c c t c a a a g a t g g c t t a c a a t t a t c t g t a g c t c c a a t t c c a g a g g a t c t g t g t g g t a a a g c g g a g t t t c t a c c g a a t g t t a a t a g a c a t c g a g g t t a a g g t c t c c t a g a c a c a c c a t t t c g

12,720 t g c a t g t a t a t g g g c a t g c a g g t a a a a c t g c t a a a c a t a t a a a a a t a g c t c a g t c t t a a g a c g t a c a t a t a c c c g t a c g t c c a t t t t g a c g a t t t g t a t a t t t t t a t c g a g t c a g a a t t c

SalI 12,780 G T G G A C T C F1ori-F a g a a t t g g t c g a c t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t c t t a a c c a g c t g a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g

12,840 T T G T T C C A A A C T G G F1ori-F t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c

12,900 a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c

12,960 AmpR promoter a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t a g g t g g c a c t t t t c g g g g a a a t g t g c g c g t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a t c c a c c g t g a a a a g c c c c t t t a c a c g c g c

13,020 AmpR promoter g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a

13,080 1 5 signal sequence MSIQHF AmpR promoter AmpR MSIQHF a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g

13,140 10 15 20 signal sequence RVALIPFFAAFCLPVFA 25 HPE AmpR RVALIPFFAAFCLPVFAHPE g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t

13,200 30 35 40 45 TLVKVKDAEDQLGARVGYIE AmpR TLVKVKDAEDQLGARVGYIE c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g

13,260 50 55 60 65 LDLNSGKILESFRPEERFPM AmpR LDLNSGKILESFRPEERFPM t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t

13,320 70 75 80 85 MSTFKVLLCGAVLSRIDAGQ AmpR MSTFKVLLCGAVLSRIDAGQ t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c

13,380 90 95 100 105 EQLGRRIHYSQNDLVEYSPV AmpR EQLGRRIHYSQNDLVEYSPV a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t

13,440 110 115 120 125 TEKHLTDGMTVRELCSAAIT AmpR TEKHLTDGMTVRELCSAAIT c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t

13,500 130 135 140 145 MSDNTAANLLLTTIGGPKEL AmpR MSDNTAANLLLTTIGGPKEL t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t

13,560 150 155 160 165 TAFLHNMGDHVTRLDRWEPE AmpR TAFLHNMGDHVTRLDRWEPE c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g

13,620 170 175 180 185 LNEAIPNDERDTTMPVAMAT AmpR LNEAIPNDERDTTMPVAMAT t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t

13,680 190 195 200 205 TLRKLLTGELLTLASRQQLI AmpR TLRKLLTGELLTLASRQQLI c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c

13,740 210 215 220 225 DWMEADKVAGPLLRSALPAG AmpR DWMEADKVAGPLLRSALPAG a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a

13,800 230 235 240 245 WFIADKSGAGERGSRGIIAA AmpR WFIADKSGAGERGSRGIIAA g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g

13,860 250 255 260 265 LGPDGKPSRIVVIYTTGSQA AmpR LGPDGKPSRIVVIYTTGSQA t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t

13,920 270 275 280 285 TMDERNRQIAEIGASLIKHW AmpR TMDERNRQIAEIGASLIKHW c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a

13,980 * AmpR * a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a

14,040 t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c

14,100 a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g

14,160 c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g g t c g a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c c a g c t c

I-SceI 14,220 t a g g g a t a a c a g g g t a a t g g a t c c t c g t t g g g c a c c t g c a c t c a t g t a c g c a g g c c c a c a a t c c c t a t t g t c c c a t t a c c t a g g a g c a a c c c g t g g a c g t g a g t a c a t g c g t c c g g g t g t

14,280 t a t a g a t g t a c a c a c g c a t a t c a t t c a a a a t a g a a a t t a t t t a t a c a a g t a g t g c g t t g g a t a t c t a c a t g t g t g c g t a t a g t a a g t t t t a t c t t t a a t a a a t a t g t t c a t c a c g c a a c c

14,340 a g a g c t g g c t c a g g a g t t g t t a a t g c t t g c t a c c c t t g c a a a g g a c c c a a g t t c a a t t t c t c t c g a c c g a g t c c t c a a c a a t t a c g a a c g a t g g g a a c g t t t c c t g g g t t c a a g t t a a a g

14,400 c a g c a c c t g t g t t g g g c a t c t c a c a a c c a c a t g t a a a t c t a g t t t c a g g g g a t t t c a c c c g t c g t g g a c a c a a c c c g t a g a g t g t t g g t g t a c a t t t a g a t c a a a g t c c c c t a a a g t g g g

14,460 c t c t t c t g a a c t c t t c a g g c a c t t a t a c c c a c a a a t g g a c a t c t c c a c a t a g a g a c a c a t g a g a a g a c t t g a g a a g t c c g t g a a t a t g g g t g t t t a c c t g t a g a g g t g t a t c t c t g t g t a

14,520 a c t t t t a a a a a a g t a a t c t a g g g t c t a g a g a a a t g g t t a t a g a c c a c g g a c t g g g a t c a g t g a a a a t t t t t t c a t t a g a t c c c a g a t c t c t t t a c c a a t a t c t g g t g c c t g a c c c t a g t c

14,580 t t c c c a g a a g c c a c a t c c t g t g g c t c a c a a t t g c c t g t g a a t c a a g t t c t a g a t c t g g t g a a g g g t c t t c g g t g t a g g a c a c c g a g t g t t a a c g g a c a c t t a g t t c a a g a t c t a g a c c a c

14,640 g c c t c g t c t a g c c t g c a g t c a t c a a c a a a c a c a t g c a c a c a t c c a t a c a c a c a t a a t t t a c g g a g c a g a t c g g a c g t c a g t a g t t g t t t g t g t a c g t g t g t a g g t a t g t g t g t a t t a a a t

14,700 a a a g a a a g c g a a a g t a a c c t a t a a t g c t a a a c a a a g c a c a t t c t c a g t g t a g a c t g c a g t t t t c t t t c g c t t t c a t t g g a t a t t a c g a t t t g t t t c g t g t a a g a g t c a c a t c t g a c g t c a

14,760 g g t g a t g a a c c t t g a a a a t a t a t t a a t a g a a g c c a g a c a c g t a a g t c a c a a a t g g t a t a a c c a c t a c t t g g a a c t t t t a t a t a a t t a t c t t c g g t c t g t g c a t t c a g t g t t t a c c a t a t t

14,820 c t c a a t t t a t a t g a a a t t c c c a g a g a g t c c a a a g t t a a a a a a a a c a a a a a g g c a g a t t a t g a g t t a a a t a t a c t t t a a g g g t c t c t c a g g t t t c a a t t t t t t t t g t t t t t c c g t c t a a t a

14,880 t g g t t g t c a g t a g c t a c a g a g a t g g g a c a t t g a a g a t a t t a a t a g g t a t a g g g t t g a g g a a c c a a c a g t c a t c g a t g t c t c t a c c c t g t a a c t t c t a t a a t t a t c c a t a t c c c a a c t c c t

14,940 t t c a t g g c c t t t c c c t c t t c c a t g t t a g c a t g t c t a t t g t t g t t a a c c t t a t t c a a c t c a a a g t a c c g g a a a g g g a g a a g g t a c a a t c g t a c a g a t a a c a a c a a t t g g a a t a a g t t g a g t

15,000 t g t t a g g g c a a t c a t a t t g a t g a g a c t t a a t g a g t a t a g c t t g a c a t t c c t a g a a g a c a g a c a a t c c c g t t a g t a t a a c t a c t c t g a a t t a c t c a t a t c g a a c t g t a a g g a t c t t c t g t c

15,060 a g t c t c a c a t c a g a c t c t c t g a t c c t c t g a c t c t t a g a a t c t t t c a a c a c c c t c t t c t g a t c a g a g t g t a g t c t g a g a g a c t a g g a g a c t g a g a a t c t t a g a a a g t t g t g g g a g a a g a c t

15,120 a a t g a t c t c t g a g c c t t t g g t t g t g a c t g a a t t a t g a g a a a g a a t t t t a t g t t g a a t g a a t t a c t a g a g a c t c g g a a a c c a a c a c t g a c t t a a t a c t c t t t c t t a a a a t a c a a c t t a c t t

15,180 t t t t a c t t c a g c a t t t t t a c a a a a c t g a a g c t g g g g a t g g c g t t t c a t g c g t g c a g t c c c a a a a t g a a g t c g t a a a a a t g t t t t g a c t t c g a c c c c t a c c g c a a a g t a c g c a c g t c a g g g

15,240 t a c c a t t t g c c t a t a a g c c t g g a g g a g g c a g a a g t a g a a t t a g g c t t t t c a g g g a c a t c t a t g g t a a a c g g a t a t t c g g a c c t c c t c c g t c t t c a t c t t a a t c c g a a a a g t c c c t g t a g a

15,300 t c a g c t g c a t c a t g a g c t t g a g g a c a a c t t g a g c t a c a t a a g a t t c c c a t c c c c a c c c c c a g t c g a c g t a g t a c t c g a a c t c c t g t t g a a c t c g a t g t a t t c t a a g g g t a g g g g t g g g g g

15,360 a c a c a c a c a c a c a c a t c t a g t a t g t a c c t a g t a t g c a g t c t t t t c a t a t g t t t a a a a t g t t g t g t g t g t g t g t g t a g a t c a t a c a t g g a t c a t a c g t c a g a a a a g t a t a c a a a t t t t a c a

15,420 a g a a a g g t a t a t g t g a a g t c t c c c t t t t c c c c a c a a c t t a g t c c c t c c a a a g t a t t g c a t t c t t t c c a t a t a c a c t t c a g a g g g a a a a g g g g t g t t g a a t c a g g g a g g t t t c a t a a c g t a

NsiI 15,480 c a g t t t c c t t g t a c a t g a t t t t a t g c a t a t a t a a g a t a c a c t a t t t c t c t c t c t c t c t c t g t c a a a g g a a c a t g t a c t a a a a t a c g t a t a t a t t c t a t g t g a t a a a g a g a g a g a g a g a g a

15,540 c t c c t t c c c t c c t t c c c t c c a a c c t c t g g g t a t g a g t t g a g g g t t a t g t a t g t t t c a a g t g a g g a a g g g a g g a a g g g a g g t t g g a g a c c c a t a c t c a a c t c c c a a t a c a t a c a a a g t t c a

15,600 t g t a a t a a g a a t t g c c a c t c a a a c a t t t c a t c c c a t t t t a a g t t a a g t t t g t c t g a t t t t a c a t t a t t c t t a a c g g t g a g t t t g t a a a g t a g g g t a a a a t t c a a t t c a a a c a g a c t a a a a

15,660 c c a c t t t a g a a g g g c a g c c a t g a t g g c t c a t g c c t a t a a t c c c a t c c t a g c a c t t g t a a g g g t g a a a t c t t c c c g t c g g t a c t a c c g a g t a c g g a t a t t a g g g t a g g a t c g t g a a c a t t c

15,720 c c t a a t a c a t a a g a c t g g a g g a t t g t c a t c a g c t t g a g g c t a g c c t g g t c t a c c t a a t g a g g a t t a t g t a t t c t g a c c t c c t a a c a g t a g t c g a a c t c c g a t c g g a c c a g a t g g a t t a c t

15,780 g t t c c a g c c a g c c a a g a g t a g a a t a t a a g a c c c t g t c t c c a a a a a a t g t a c c a a a g g g g c c a a g g t c g g t c g g t t c t c a t c t t a t a t t c t g g g a c a g a g g t t t t t t a c a t g g t t t c c c c g

15,840 t g g a g g g g t a a c t c a g t g g t t a a g a a c a g t a g c t g c t c t t c c a g a g g a c c t g g g t t c a a t a c c t c c c c a t t g a g t c a c c a a t t c t t g t c a t c g a c g a g a a g g t c t c c t g g a c c c a a g t t a

15,900 t c c c a g c a c c c a c a t g g c a g c t t g c a a c t a t c t g t a a c t g t c c a g c t t t g g g a t a t c c g a a g g g t c g t g g g t g t a c c g t c g a a c g t t g a t a g a c a t t g a c a g g t c g a a a c c c t a t a g g c t

15,960 c a c c a t t a t a c c a a t t a c a t a a a a t g a a a a a a t t g t a a a a t g t g c t a a a a t g a g t g g g g a g t g g t a a t a t g g t t a a t g t a t t t t a c t t t t t t a a c a t t t t a c a c g a t t t t a c t c a c c c c t

16,020 g g g g g a g c a t g g t g t g g t t c t g t g g g c t t g g g a t t t t g c c a t g a a t c c a t g a c t a t a t c t c c c c c t c g t a c c a c a c c a a g a c a c c c g a a c c c t a a a a c g g t a c t t a g g t a c t g a t a t a g a

16,080 c a g c a a t a t g t g g a t t a t t t c t g g a a a c c t g t g g g g c a t g g g t g t a t g g g a a g a g g g g a g g t c g t t a t a c a c c t a a t a a a g a c c t t t g g a c a c c c c g t a c c c a c a t a c c c t t c t c c c c t c

16,140 a g a g a g a g a g c c c g c a t c c a g c c a g a g t t c c t g t g c t c t g g g c a g g c g g a c g t g g g a g g a t c t c t c t c t c g g g c g t a g g t c g g t c t c a a g g a c a c g a g a c c c g t c c g c c t g c a c c c t c c t

16,200 c t g c t g g a c g a t t t c c a c t t g g c c c t g g g t g g g c a t c t g g c t g t g t g a a g c c a c t g a c c c g a c g a c c t g c t a a a g g t g a a c c g g g a c c c a c c c g t a g a c c g a c a c a c t t c g g t g a c t g g g

16,260 c a c t c a g c g g g g g g t a g a t a a g g g g c a t t t c c c a t a c c a g g t t c t c a g a c t c c a g c a t t c g t g a g t c g c c c c c c a t c t a t t c c c c g t a a a g g g t a t g g t c c a a g a g t c t g a g g t c g t a a g

16,320 c a c g c t g g a t a c a g c a g a a g a g c t g a g a a c a g a a t a g g g g c c c c a g g g a g a c a c t c t c g t g t g c g a c c t a t g t c g t c t t c t c g a c t c t t g t c t t a t c c c c g g g g t c c c t c t g t g a g a g c a

16,380 c c c a g a g a t a a a g a a g g c a g a g g g a g a g a g a t t c c t a c g c a g g c g a g a g g c c t t a g t c t g g g g t c t c t a t t t c t t c c g t c t c c c t c t c t c t a a g g a t g c g t c c g c t c t c c g g a a t c a g a c

16,440 g g c c t t g a t g a a c a g a g a c g g t c t a t g g t t t t a g a g c t t t a t t g t a g a a a g g c a g g g g g a c c g g a a c t a c t t g t c t c t g c c a g a t a c c a a a a t c t c g a a a t a a c a t c t t t c c g t c c c c c t

16,500 a a g a g a g a a g g t a g a a a g a g a g a g a c c a g c c a t g g c c a a g a g g a g a g a a g g g g g g a a a g g t t c t c t c t t c c a t c t t t c t c t c t c t g g t c g g t a c c g g t t c t c c t c t c t t c c c c c c t t t c c

16,560 a g a g a g a g a a g a a a g g c t a g a g a g t a a g a a a g g t t a a a g a g a c a g a a g t a a a a g c t t a g a t c t c t c t c t t c t t t c c g a t c t c t c a t t c t t t c c a a t t t c t c t g t c t t c a t t t t c g a a t c t

16,620 g t a a g a g a g a g a g a g a g a g c a a g a g g g a g a g t a a g a a a a g t a a a a g c t t a a g a g a g c g a g c a t t c t c t c t c t c t c t c t c g t t c t c c c t c t c a t t c t t t t c a t t t t c g a a t t c t c t c g c t c

16,680 g a g g g g c c a g c c c c t c t a a t a g t g g g c t t g t t a t c t t g t t g t t g c t a g g t a a c t g t g a a g c t c c c c g g t c g g g g a g a t t a t c a c c c g a a c a a t a g a a c a a c a a c g a t c c a t t g a c a c t t c

16,740 c g t c t a g c c t g a a g g c c a g a a g c t t g g g a c a t t g t c t a t g t g a c t g a a a g c c a t g a g c c t g c a g a t c g g a c t t c c g g t c t t c g a a c c c t g t a a c a g a t a c a c t g a c t t t c g g t a c t c g g a

16,800 c t a c t g t g g g g g c t g t g g a g g t g g t a a c t t g g a c a g g a g c c a g g g g t t c c a g g a g a c a t g g a t g a c a c c c c c g a c a c c t c c a c c a t t g a a c c t g t c c t c g g t c c c c a a g g t c c t c t g t a c

16,860 a g g g a a t g c c t t c t g t c c c a t g t a g g t a g g a a t t a c c a c c a c c g g c t t t c a c c g t a g t t c t c c c t t a c g g a a g a c a g g g t a c a t c c a t c c t t a a t g g t g g t g g c c g a a a g t g g c a t c a a g

16,920 a c t g g a g a c t g t t t g t a c a t a g c c c a t t g c c c c a c a g a a a c c a g g c a c t c a c t g t t t c a t t g a c c t c t g a c a a a c a t g t a t c g g g t a a c g g g g t g t c t t t g g t c c g t g a g t g a c a a a g t a

16,980 t t g c a t a a g a a t t a g a a g g c t t t a t t t t g g c t g g g a t t g g t g a c t t g c c c c a g a a a t t c c a a c g t a t t c t t a a t c t t c c g a a a t a a a a c c g a c c c t a a c c a c t g a a c g g g g t c t t t a a g g

17,040 a g c t t t t g g g a a g c c g a a t c c t g g a g a a c c a g g a g t t c a a g g t c a t c c t c a g g t a c c t c a t c g a a a a c c c t t c g g c t t a g g a c c t c t t g g t c c t c a a g t t c c a g t a g g a g t c c a t g g a g t

17,100 t t t g t t c a a g a c c a g c c t g t g c t a c c a t g a g a c c t t g t c c c t a c t c c c c t c a a c c c c a a a a a a c a a g t t c t g g t c g g a c a c g a t g g t a c t c t g g a a c a g g g a t g a g g g g a g t t g g g g t t t

17,160 a c a c g c t g t t t t c a t t t t a t a g g t t g t a c t a a c c a t g g a g g a t g g t c a t t t g c c t t t c t c t g t g c g a c a a a a g t a a a a t a t c c a a c a t g a t t g g t a c c t c c t a c c a g t a a a c g g a a a g a g

17,220 t t g a t t t c a g a a g c g t a g c g c g g g c t t t g c a c c a t g a t c t a g t g t t t c c a g a t a g g c t t g a a c t a a a g t c t t c g c a t c g c g c c c g a a a c g t g g t a c t a g a t c a c a a a g g t c t a t c c g a a c

17,280 c c t c t c c a c a g c c a g g c t t a t c c t g c t t c a g t c c c a t g t t t g g c a a g g g c a a c a t a t g a c g g a g a g g t g t c g g t c c g a a t a g g a c g a a g t c a g g g t a c a a a c c g t t c c c g t t g t a t a c t g

17,340 t g a a g a a c t t g c a t a g c c t t a t g g t g t a t c a t g a c t c t t a g c c c c a g t t t a t a a c t g t c t a c t t c t t g a a c g t a t c g g a a t a c c a c a t a g t a c t g a g a a t c g g g g t c a a a t a t t g a c a g a

17,400 a a c a g c t g g t g g c t t t t g t g a a a a a g g a a a g c a t c a t t t g a a c t t t t a a c t t t t c a g c t g t t g t c g a c c a c c g a a a a c a c t t t t t c c t t t c g t a g t a a a c t t g a a a a t t g a a a a g t c g a c

17,460 t t t g t t t t g a c t t t g c a t a a a t a c t g a g a c t a g c t a g g a a a t g a g c t t t t g t t t c a t t t g a a a c a a a a c t g a a a c g t a t t t a t g a c t c t g a t c g a t c c t t t a c t c g a a a a c a a a g t a a a c

17,520 c t a a a a a t g a t t t t t t t t t c a t g a g g c a a t t t c a a g c a a t t t g a a t t t a g c a a t c t c t a c g a t t t t t a c t a a a a a a a a a g t a c t c c g t t a a a g t t c g t t a a a c t t a a a t c g t t a g a g a t g

17,580 t t g t c t g a a a a g a a g t t a t t a a t t a a a a a a a a a c a a a c a a a c a g g a a c c t a t t a t g c t g g a a c a g a c t t t t c t t c a a t a a t t a a t t t t t t t t t g t t t g t t t g t c c t t g g a t a a t a c g a c c

17,640 c t a g t c a c a t g a a a g a a t c c a a a t g a a c a c a g t c a a g g c t g a a t c a a c a c a t g a a a t t a t g a t c a g t g t a c t t t c t t a g g t t t a c t t g t g t c a g t t c c g a c t t a g t t g t g t a c t t t a a t a

17,700 t t t c t a a t t c c c c a a a t t a c a t t t a t g c t a a t t a g t a t g t a g t a a a a c a t t t t a a t t g t t a a a g a t t a a g g g g t t t a a t g t a a a t a c g a t t a a t c a t a c a t c a t t t t g t a a a a t t a a c a a

17,760 t c c a a t g t t a a a c a t t a c a g a a a t a a t t t t g a t g t t t t c c t a a t a a c c c a g c c t t t g a t c a g g t t a c a a t t t g t a a t g t c t t t a t t a a a a c t a c a a a a g g a t t a t t g g g t c g g a a a c t a g

17,820 a t g t t t g c a t t t a t t t g a t t g t t g t c a g t t t a t t a a a t a a t t a a g t a a g a g c a a c a a g a t t a c a a a c g t a a a t a a a c t a a c a a c a g t c a a a t a a t t t a t t a a t t c a t t c t c g t t g t t c t a

17,880 a a a a t g a c t t c a t g a g a g t c c t t t t c t g t a a c t t a g a a c g t c a g t a g t c a t a g g a a c t g c t t t t a c t g a a g t a c t c t c a g g a a a a g a c a t t g a a t c t t g c a g t c a t c a g t a t c c t t g a c g

17,940 g g t c g t t c c c t t a c c t t t c a g a a g t a g g c t a a a g a g t t g a a c g c a a a g g t g g t t t t t c g a c c a g c a a g g g a a t g g a a a g t c t t c a t c c g a t t t c t c a a c t t g c g t t t c c a c c a a a a a g c t

18,000 a t c t t c c t g a t c c t t a a g c c c c c a c t a g g t t t a t a t t c t a t a t t t g a t a t c g g a t t c g a a t a g a a g g a c t a g g a a t t c g g g g g t g a t c c a a a t a t a a g a t a t a a a c t a t a g c c t a a g c t t

PspXI 18,060 c c c c t c g a g c t t a g g a t c c g g a a c c c t t a a t a t a a c t a a g t t a t t a g g t c c c t c g a a g a g g g g g a g c t c g a a t c c t a g g c c t t g g g a a t t a t a t t g a t t c a a t a a t c c a g g g a g c t t c t c

18,120 g t t c a c t a g t a c t g g c c a t t g c g g c c g c t t c g a g g c t g c c t c g c g c g t t t c g g t g a t g a c c a a g t g a t c a t g a c c g g t a a c g c c g g c g a a g c t c c g a c g g a g c g c g c a a a g c c a c t a c t g

18,180 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a c a g c t t g t c t g t a a g c g g a t c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t g t c g a a c a g a c a t t c g c c t a

18,240 bom g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g t t g g c g g g t g t c g g g g c g c a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c a a c c g c c c a c a g c c c c g c g t

18,300 bom C G G C A T C A G pRS-marker g c c a t g a c c c a g t c a c g t a g c g a t a g c g g a g t g t a t a c t g g c t t a a c t a t g c g g c a t c a g c g g t a c t g g g t c a g t g c a t c g c t a t c g c c t c a c a t a t g a c c g a a t t g a t a c g c c g t a g t c

18,360 bom A G C A G A T T G T A pRS-marker a g c a g a t t g t a c t g a g a g t g c a c c a t a t g c g g t g t g a a a t a c c g c a c a g a t g c g t a a g g a t c g t c t a a c a t g a c t c t c a c g t g g t a t a c g c c a c a c t t t a t g g c g t g t c t a c g c a t t c c t

18,420 bom G A G C G A G T G A C T G A G C G A L4440 g a a a a t a c c g c a t c a g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g c t t t t a t g g c g t a g t c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c

18,480 t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a

18,540 c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t

18,600 ori a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t

18,660 ori T T T C T A T G G T C C G C A A A G pBR322ori-F a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g

18,720 ori G G pBR322ori-F c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a

18,780 ori c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t

18,840 ori g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c

18,900 ori a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a

18,960 ori c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t

19,020 ori c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g g a c a g t a t t t g g t a t c t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c c t g t c a t a a a c c a t a g a

19,080 ori g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g

19,140 ori a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t

19,200 ori a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t

19,260 a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a

19,320 t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t

19,380 285 280 275 270 *WHKILSAGIEAIQRNREDM AmpR *WHKILSAGIEAIQRNREDM g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t

19,440 265 260 255 250 TAQSGTTYIVVIRSPKGDPG AmpR TAQSGTTYIVVIRSPKGDPG t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g

19,500 245 240 235 230 LAAIIGRSGREGAGSKDAIF AmpR LAAIIGRSGREGAGSKDAIF c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t

19,560 225 220 215 210 WGAPLASRLLPGAVKDAEMW AmpR WGAPLASRLLPGAVKDAEMW a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g

19,620 205 200 195 190 DILQQRSALTLLEGTLLKRL AmpR DILQQRSALTLLEGTLLKRL a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t

19,680 185 180 175 170 TTAMAAPMTTDREDNPIAEN AmpR TTAMAAPMTTDREDNPIAEN a c g t t g t t g c c a t t g c t g c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t g c a a c a a c g g t a a c g a c g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a

19,740 165 160 155 150 LEPEWRDLRTVHDGMNHLFA AmpR LEPEWRDLRTVHDGMNHLFA t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c

19,800 145 140 135 130 TLEKPGGITTLLLNAATNDS AmpR TLEKPGGITTLLLNAATNDS c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g

19,860 125 120 115 110 MTIAASCLERVTMGDTLHKE AmpR MTIAASCLERVTMGDTLHKE t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a

19,920 105 100 95 90 TVPSYEVLDNQSYHIRRGLQ AmpR TVPSYEVLDNQSYHIRRGLQ c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a

19,980 85 80 75 70 EQGADVRSLVAGCLLVKFTS AmpR EQGADVRSLVAGCLLVKFTS g c t c t t g c c c g g c g t c a a c a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c c g a g a a c g g g c c g c a g t t g t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g

20,040 65 60 55 50 MMPFREEPRFSELIKGSNLD AmpR MMPFREEPRFSELIKGSNLD t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a

20,100 45 40 35 30 LEIYGVRAGLQDEADKVKVL AmpR LEIYGVRAGLQDEADKVKVL c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t

20,160 25 TEPH 20 15 10 signal sequence AFVPLCFAAFFPILAV AmpR TEPHAFVPLCFAAFFPILAV g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t

20,220 5 1 signal sequence RFHQISM AmpR AmpR promoter RFHQISM c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c

20,280 AmpR promoter g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c

3ʹ 5ʹ 20,317 AmpR promoter t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g g a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.