Addgene MSP2443 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: MSP2443 Sequencing Result

Map View

800 600 400 200 End (996) HincII (962) BsgI (921) XmnI (912) BtgZI (892) KflI - PpuMI - EcoO109I (857) BmrI (855) BanII - Bsp1286I (833) AfeI (727) BpuEI (675) EaeI (610) TsoI (609) BmtI (595) NheI (591) EcoRI (411) BsaBI (384) SspI (314) EciI (309) NmeAIII (307) BpmI (296) BsmI (273) TatI (230) BglII (225) StyI (218) BamHI (129) EarI (121) AvaI - XhoI - BsoBI - PaeR7I (103) MslI (84) PsiI (75) BspHI * (64) PstI (33) AgeI - BsrFI (19) Start (0) 6xHis 3xFLAG SV40 NLS MSP2443 996 bp

Sequence View

PstIBsrFIAgeIStart (0) 5ʹ 3ʹ 60 5 1 HHHHHH *GAA 20 15 KDDDDKYD 6xHis 3xFLAG *GAAKDDDDKYD a t g g t g a t g g t g a t g a t g a c c g g t c a t c c t g c a g c c t t g t c a t c g t c a t c c t t g t a a t c g t a c c a c t a c c a c t a c t a c t g g c c a g t a g g a c g t c g g a a c a g t a g c a g t a g g a a c a t t a g c

PaeR7IBsoBIXhoIAvaIMslIPsiIBspHI* 120 10 5 1 IDHDKYDGDHDKYD SS 130 VKRK 3xFLAG SV40 NLS IDHDKYDGDHDKYDSSVKRK a t g t c a t g a t c t t t a t a a t c a c c g t c a t g g t c t t t g t a g t c g c t c g a g a c t t t c c t c t t c t a c a g t a c t a g a a a t a t t a g t g g c a g t a c c a g a a a c a t c a g c g a g c t c t g a a a g g a g a a g

BamHIEarI 180 KKP SV40 NLS KKPSGDGGLQSLDIRTEYLG t t c t t g g g g g a t c c g t c a c c c c c a a g c t g t g a c a a a t c t a t c c g a g t t t c a t a t a a t c c c a a g a a c c c c c t a g g c a g t g g g g g t t c g a c a c t g t t t a g a t a g g c t c a a a g t a t a t t a g g g

TatIBglIIStyI 240 TISQHILTADLVEKTSRYQK g t g a t g g a t t g g t g a a t c a g t g t c g c g t c t a g c a c c t c c t t g g t a g a t c t g t a c t g t t t g c a c t a c c t a a c c a c t t a g t c a c a g c g c a g a t c g t g g a g g a a c c a t c t a g a c a t g a c a a a c

BpmIBsmI 300 RDITTDFYKFAAPAGLNTLT c g a t c t a t c g t t g t g t c a a a a t a c t t g a a t g c g g c t g g a g c g c c g a g g t t g g t a a g a g t a g c t a g a t a g c a a c a c a g t t t t a t g a a c t t a c g c c g a c c t c g c g g c t c c a a c c a t t c t c a t

SspINmeAIIIEciI 360 FLHIINEAQERIPKDRHKNY a a c a a a t g g a t a a t a t t t t c c g c c t g c t c a c g t a t g g g t t t a t c c c t g t g c t t g t t g t a t t t g t t t a c c t a t t a t a a a a g g c g g a c g a g t g c a t a c c c a a a t a g g g a c a c g a a c a a c a t a

EcoRIBsaBI 420 ASLVKDLNADALIVRKSFES g c g c t t a a t a c t t t g t c c a g a t t g g c a t c a g c t a g g a t g a c t c t c t t a c t g a a t t c c g a a c g c g a a t t a t g a a a c a g g t c t a a c c g t a g t c g a t c c t a c t g a g a g a a t g a c t t a a g g c t t

480 IQEIIEDLYHKHQEVFLQKQ a t t t g c t c t a t g a t t t c g t c g a g a t a a t g t t t g t g c t g c t c a a c a a a a a g t t g c t t c t g t t a a a c g a g a t a c t a a a g c a g c t c t a t t a c a a a c a c g a c g a g t t g t t t t t c a a c g a a g a c a

540 ENDEPSGKLKEYHSALYLFN t c g t t a t c t t c a g g t g a a c c t t t c a a c t t c t c g t a a t g g g a c g c t a a a t a c a g g a a a t t c a g c a a t a g a a g t c c a c t t g g a a a g t t g a a g a g c a t t a c c c t g c g a t t t a t g t c c t t t a a g

BmtINheI 600 VYKSPLALENGKQLERASAL a c g t a t t t a g a c g g t a g t g c g a g t t c g t t c c c c t t t t g a a g c t c t c t g g c g c t a g c c a a c t g c a t a a a t c t g c c a t c a c g c t c a a g c a a g g g g a a a a c t t c g a g a g a c c g c g a t c g g t t g

TsoIEaeI 660 MRKRGNELEFLSYKPLKIIL a t c c g t t t t c g g c c a t t t t c t a a c t c a a a c a g a c t a t a c t t t g g t a g t t t a a t t a t g a g a t a g g c a a a a g c c g g t a a a a g a t t g a g t t t g t c t g a t a t g a a a c c a t c a a a t t a a t a c t c t

BpuEI 720 DKKVEKYGKAELFDIPNKEF t c c t t t t t t a c t t c c t t g t a a c c t t t c g c c t c a a g g a a g t c g a t g g g g t t c t t t t c a a a a a g g a a a a a a t g a a g g a a c a t t g g a a a g c g g a g t t c c t t c a g c t a c c c c a a g a a a a g t t t t

AfeI 780 SSREMITIGLLEKVSKLKKS g a c g a g c g c t c c a t a a t c g t t a t c c c c a a t a a t t c t t t g a c t g a c t t c a g t t t c t t g g a t c t g c t c g c g a g g t a t t a g c a a t a g g g g t t a t t a a g a a a c t g a c t g a a g t c a a a g a a c c t a

Bsp1286IBanII 840 KGKEVKAVVLVSYAVTPSVF t t t c c c t t c t c a a c t t t t g c c a c t a c t a g g a c a g a a t a g g c a a c t g t a g g g c t c a c g a a g a a a g g g a a g a g t t g a a a a c g g t g a t g a t c c t g t c t t a t c c g t t g a c a t c c c g a g t g c t t c

BtgZIBmrIEcoO109IPpuMIKflI 900 GGYKKPDWDKKRAILKDSNR c c a c c g t a c t t t t t c g g g t c c c a g t c c t t t t t a c g a g c g a t g a g c t t a t c a c t a t t c c t t g g t g g c a t g a a a a a g c c c a g g g t c a g g a a a a a t g c t c g c t a c t c g a a t a g t g a t a a g g a a

BsgIXmnI 960 KPLISEKSFGGTQVETKKVI t t t g g a a g a a t c g a t t c c t t t g a a a a c c c t c c g g t c t g c a c c t c a g t t t t c t t t a c t a t g a a a c c t t c t t a g c t a a g g a a a c t t t t g g g a g g c c a g a c g t g g a g t c a a a a g a a a t g a t a c

End (996) HincII 3ʹ 5ʹ 996 NVQPMSLVKRVT t t g a c t t g g g g c a t g g a c a a a a c t t t t c t c a c c g t c a a c t g a a c c c c g t a c c t g t t t t g a a a a g a g t g g c a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.