Addgene pAS1NB c Rosella I Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pAS1NB c Rosella I Sequencing Result


Map View

800 600 400 200 End (975) AleI (896) DsRed1-C (861 .. 884) BsgI (848) PvuII (832) MscI (815) BstXI (811) PflMI * (787) CsiI - SexAI * (786) EcoO109I (757) HaeII (715) PspFI (697) BseYI (693) MmeI (684) mCherry-F (667 .. 686) BsiEI (616) EagI (613) SbfI (609) DsRed1-N (449 .. 469) BmrI (451) AhdI (448) mCherry-R (399 .. 417) FspI - FspAI (317) AatII (288) ZraI (286) BsaHI (285) BglI (269) NcoI (267) SpeI (256) BamHI (248) SmaI (246) ApaI (245) XmaI - TspMI (244) PspOMI (241) SacII (240) KpnI (237) Acc65I (233) HincII (229) AccI (228) SalI (227) ApoI - EcoRI (217) BstBI (215) HindIII (210) SacI - BsiHKAI (208) Eco53kI (206) XhoI - PaeR7I (201) NspI (199) PciI - AflIII (195) BpuEI (192) XbaI (181) Start (0) MCS DsRed-Express Kozak sequence pAS1NB c Rosella I 975 bp

Sequence View

Start (0) 60 g g g t g g t t t a g t t t a g t a g a a c c t c g t g a a a c t t a c a t t t a c a t a t a t a t a a a c t t g c a t c c c a c c a a a t c a a a t c a t c t t g g a g c a c t t t g a a t g t a a a t g t a t a t a t a t t t g a a c g t a

120 a a a t t g g t c a a t g c a a g a a a t a c a t a t t t g g t c t t t t c t a a t t c g t a g t t t t t c a a g t t c t t t a a c c a g t t a c g t t c t t t a t g t a t a a a c c a g a a a a g a t t a a g c a t c a a a a a g t t c a a g

180 t t a g a t g c t t t c t t t t t c t c t t t t t t a c a g a t c a t c a a g g a a g t a a t t a t c t a c t t t t t c a a t c t a c g a a a g a a a a a g a g a a a a a a t g t c t a g t a g t t c c t t c a t t a a t a g a t g a a a a a g

SalIAccIHincIIAcc65IKpnISacIIBpuEIAflIIIPciINspIPaeR7IXhoIEco53kIBsiHKAISacIHindIIIBstBIEcoRIApoIXbaI 240 MCS MSRAQASNSAVDGTA t c t a g a a g a t c t a a a c a t g t c t c g a g c t c a a g c t t c g a a t t c t g c a g t c g a c g g t a c c g c a g a t c t t c t a g a t t t g t a c a g a g c t c g a g t t c g a a g c t t a a g a c g t c a g c t g c c a t g g c g

BsaHIZraIAatIINcoIBglISpeIPspOMITspMIXmaIApaISmaIBamHI 300 1 5 10 MASSEDVIKEF MCS DsRed-Express Kozak sequence GPGSPLVATMASSEDVIKEF g g g c c c g g g a t c c c c a c t a g t c g c c a c c a t g g c c t c c t c c g a g g a c g t c a t c a a g g a g t t c c c g g g c c c t a g g g g t g a t c a g c g g t g g t a c c g g a g g a g g c t c c t g c a g t a g t t c c t c a a

FspAIFspI 360 15 20 25 30 MRFKVRMEGSVNGHEFEIEG DsRed-Express MRFKVRMEGSVNGHEFEIEG c a t g c g c t t c a a g g t g c g c a t g g a g g g c t c c g t g a a c g g c c a c g a g t t c g a g a t c g a g g g g t a c g c g a a g t t c c a c g c g t a c c t c c c g a g g c a c t t g c c g g t g c t c a a g c t c t a g c t c c c

420 35 40 45 50 EGEGRPYEGTQTAKLKVTKG DsRed-Express G G T T C G A C T T C C A C T G G T T mCherry-R EGEGRPYEGTQTAKLKVTKG c g a g g g c g a g g g c c g c c c c t a c g a g g g c a c c c a g a c c g c c a a g c t g a a g g t g a c c a a g g g g c t c c c g c t c c c g g c g g g g a t g c t c c c g t g g g t c t g g c g g t t c g a c t t c c a c t g g t t c c c

AhdIBmrI 480 55 60 65 70 GPLPFAWDILSPQFQYGSKV DsRed-Express G A C A G G G G G G T C A A G G T C A T G DsRed1-N GPLPFAWDILSPQFQYGSKV c g g c c c c c t g c c c t t c g c c t g g g a c a t c c t g t c c c c c c a g t t c c a g t a c g g c t c c a a g g t g c c g g g g g a c g g g a a g c g g a c c c t g t a g g a c a g g g g g g t c a a g g t c a t g c c g a g g t t c c a

540 75 80 85 90 YVKHPADIPDYKKLSFPEGF DsRed-Express YVKHPADIPDYKKLSFPEGF g t a c g t g a a g c a c c c c g c c g a c a t c c c c g a c t a c a a g a a g c t g t c c t t c c c c g a g g g c t t c a t g c a c t t c g t g g g g c g g c t g t a g g g g c t g a t g t t c t t c g a c a g g a a g g g g c t c c c g a a

600 95 100 105 110 KWERVMNFEDGGVVTVTQDS DsRed-Express KWERVMNFEDGGVVTVTQDS c a a g t g g g a g c g c g t g a t g a a c t t c g a g g a c g g c g g c g t g g t g a c c g t g a c c c a g g a c t c g t t c a c c c t c g c g c a c t a c t t g a a g c t c c t g c c g c c g c a c c a c t g g c a c t g g g t c c t g a g

SbfIEagIBsiEI 660 115 120 125 130 SLQDGRFIYKVKFIGVNFPS DsRed-Express SLQDGRFIYKVKFIGVNFPS c t c c c t g c a g g a c g g c c g c t t c a t c t a c a a g g t g a a g t t c a t c g g c g t g a a c t t c c c c t c g a g g g a c g t c c t g c c g g c g a a g t a g a t g t t c c a c t t c a a g t a g c c g c a c t t g a a g g g g a g

HaeIIBseYIPspFIMmeI 720 135 140 145 150 DGPVMQKKTMGWEPSTERLY DsRed-Express C C C C G T A A T G C A G A A G A A G A mCherry-F DGPVMQKKTMGWEPSTERLY c g a c g g c c c c g t a a t g c a g a a g a a g a c t a t g g g c t g g g a g c c c t c c a c c g a g c g c c t g t a g c t g c c g g g g c a t t a c g t c t t c t t c t g a t a c c c g a c c c t c g g g a g g t g g c t c g c g g a c a t

EcoO109I 780 155 160 165 170 PRDGVLKGEIHKALKLKDGG DsRed-Express PRDGVLKGEIHKALKLKDGG c c c c c g c g a c g g c g t g c t g a a g g g c g a g a t c c a c a a g g c c c t g a a g c t g a a g g a c g g c g g g g g g g c g c t g c c g c a c g a c t t c c c g c t c t a g g t g t t c c g g g a c t t c g a c t t c c t g c c g c c

PvuIIBstXIMscISexAI*CsiIPflMI* 840 175 180 185 190 HYLVEFKSIYMAKKPVQLPG DsRed-Express HYLVEFKSIYMAKKPVQLPG c c a c t a c c t g g t g g a g t t c a a g t c c a t c t a c a t g g c c a a g a a g c c c g t g c a g c t g c c c g g g g t g a t g g a c c a c c t c a a g t t c a g g t a g a t g t a c c g g t t c t t c g g g c a c g t c g a c g g g c c

AleIBsgI 900 195 200 205 210 YYYVDSKLDITSHNEDYTIV DsRed-Express A G C T G G A C A T C A C C T C C C A C A A C G DsRed1-C YYYVDSKLDITSHNEDYTIV c t a c t a c t a c g t g g a c t c c a a g c t g g a c a t c a c c t c c c a c a a c g a g g a c t a c a c c a t c g t g a t g a t g a t g c a c c t g a g g t t c g a c c t g t a g t g g a g g g t g t t g c t c c t g a t g t g g t a g c a

960 215 220 225 EQYERTEGRHHLFL (in frame with DsRed-Express) SDPWSS DsRed-Express EQYERTEGRHHLFLSDPWSS g g a g c a g t a c g a g c g c a c c g a g g g c c g c c a c c a c c t g t t c c t g t c t g a t c c c t g g t c t t c c c t c g t c a t g c t c g c g t g g c t c c c g g c g g t g g t g g a c a a g g a c a g a c t a g g g a c c a g a a g

End (975) 975 SGDL SGDL t t c g g g a g a t c t a a a a a g c c c t c t a g a t t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.