Addgene pACUH-GFP11x7-mCherry-α-tubulin Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pACUH-GFP11x7-mCherry-α-tubulin Sequencing Result

Map View

600 400 200 End (701) StuI (691) AhdI (660) AcuI (637) mCherry-R (611 .. 629) BstEII (621) BanI (598) BssSI - BssSαI (553) BanII (541) BsiHKAI (530) ApaLI (526) MscI - BseRI (496) SgrAI (451) BsgI (406) BsiEI (405) PvuII - MspA1I (316) SnaBI (306) BsaI (275) DraIII (228) BspEI (208) HpaI - HincII (120) BpmI (104) PspFI (68) BseYI (64) BspHI (45) EcoRI (19) Start (0) GFP11x7 pACUH-GFP11x7-mCherry- α- tubulin 701 bp

Sequence View

BspHIEcoRIStart (0) 5ʹ 3ʹ 60 1 5 10 RDHMVLHEYVN GFP11x7 MRDHMVLHEYVN c t c t g a a t a g g g a a t t g g g a a t t c a t g c g t g a c c a c a t g g t c c t t c a t g a g t a t g t a a a t g a g a c t t a t c c c t t a a c c c t t a a g t a c g c a c t g g t g t a c c a g g a a g t a c t c a t a c a t t t a

HincIIHpaIBpmIBseYIPspFI 120 15 20 25 30 AAGITGGSGGRDHMVLHEYV GFP11x7 AAGITGGSGGRDHMVLHEYV g c t g c t g g g a t t a c a g g t g g c t c t g g a g g t a g a g a t c a t a t g g t t c t c c a c g a a t a c g t t c g a c g a c c c t a a t g t c c a c c g a g a c c t c c a t c t c t a g t a t a c c a a g a g g t g c t t a t g c a a

180 35 40 45 50 NAAGITGGSGGRDHMVLHEY GFP11x7 NAAGITGGSGGRDHMVLHEY a a c g c c g c a g g c a t c a c t g g c g g t a g t g g a g g a c g c g a c c a t a t g g t a c t a c a t g a a t a t t t g c g g c g t c c g t a g t g a c c g c c a t c a c c t c c t g c g c t g g t a t a c c a t g a t g t a c t t a t a

DraIIIBspEI 240 55 60 65 70 VNAAGITGGSGGRDHMVLHE GFP11x7 VNAAGITGGSGGRDHMVLHE g t c a a t g c a g c c g g a a t a a c c g g a g g g t c c g g a g g c c g g g a t c a c a t g g t g c t g c a t g a g c a g t t a c g t c g g c c t t a t t g g c c t c c c a g g c c t c c g g c c c t a g t g t a c c a c g a c g t a c t c

BsaI 300 75 80 85 90 YVNAAGITGGSGGRDHMVLH GFP11x7 YVNAAGITGGSGGRDHMVLH t a t g t g a a c g c g g c g g g t a t a a c t g g t g g g t c g g g c g g a c g a g a c c a t a t g g t g c t t c a c a t a c a c t t g c g c c g c c c a t a t t g a c c a c c c a g c c c g c c t g c t c t g g t a t a c c a c g a a g t g

MspA1IPvuIISnaBI 360 95 100 105 110 EYVNAAGITGGSGGRDHMVL GFP11x7 EYVNAAGITGGSGGRDHMVL g a a t a c g t a a a c g c a g c t g g c a t t a c t g g c g g a t c a g g t g g c a g g g a t c a c a t g g t a c t c c t t a t g c a t t t g c g t c g a c c g t a a t g a c c g c c t a g t c c a c c g t c c c t a g t g t a c c a t g a g

BsiEIBsgI 420 115 120 125 130 HEYVNAAGITGGSGGRDHMV GFP11x7 HEYVNAAGITGGSGGRDHMV c a t g a g t a c g t g a a c g c t g c t g g a a t c a c a g g c g g t a g c g g c g g t c g g g a c c a t a t g g t c g t a c t c a t g c a c t t g c g a c g a c c t t a g t g t c c g c c a t c g c c g c c a g c c c t g g t a t a c c a g

SgrAI 480 135 140 LHEYVNAAGIT (in frame with GFP11x7) GGKFMVSKG GFP11x7 LHEYVNAAGITGGKFMVSKG c t g c a c g a a t a t g t c a a t g c t g c c g g t a t c a c c g g c g g c a a a t t c a t g g t g a g c a a g g g c g a c g t g c t t a t a c a g t t a c g a c g g c c a t a g t g g c c g c c g t t t a a g t a c c a c t c g t t c c c g

ApaLIBsiHKAIBseRIMscI 540 (in frame with GFP11x7) EEDNMAIIKEFMRFKVHMEG EEDNMAIIKEFMRFKVHMEG g a g g a g g a t a a c a t g g c c a t c a t c a a g g a g t t c a t g c g c t t c a a g g t g c a c a t g g a g g g c c t c c t c c t a t t g t a c c g g t a g t a g t t c c t c a a g t a c g c g a a g t t c c a c g t g t a c c t c c c g

BanIBssSαIBssSIBanII 600 (in frame with GFP11x7) SVNGHEFEIEGEGEGRPYEG SVNGHEFEIEGEGEGRPYEG t c c g t g a a c g g c c a c g a g t t c g a g a t c g a g g g c g a g g g c g a g g g c c g c c c c t a c g a g g g c a g g c a c t t g c c g g t g c t c a a g c t c t a g c t c c c g c t c c c g c t c c c g g c g g g g a t g c t c c c g

AhdIAcuIBstEII 660 (in frame with GFP11x7) TQTAKLKVTKGGPLPFAWDI G G T T C G A C T T C C A C T G G T T mCherry-R TQTAKLKVTKGGPLPFAWDI a c c c a g a c c g c c a a g c t g a a g g t g a c c a a g g g t g g c c c c c t g c c c t t c g c c t g g g a c a t c t g g g t c t g g c g g t t c g a c t t c c a c t g g t t c c c a c c g g g g g a c g g g a a g c g g a c c c t g t a g

End (701) StuI 3ʹ 5ʹ 701 (in frame with GFP11x7) LSPQFMYGSKAYV LSPQFMYGSKAYV c t g t c c c c t c a g t t c a t g t a c g g c t c c a a g g c c t a c g t g a a g a c a g g g g a g t c a a g t a c a t g c c g a g g t t c c g g a t g c a c t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.