Addgene pFA6a-HBH-kanMX6 - Sequence Analyzer Skip to main content
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Sequence Analyzer: pFA6a-HBH-kanMX6

Map View

SP6 (4431 .. 1) NdeI (4364) pRS-marker (4331 .. 4350) pGEX 3' (4209 .. 4231) PfoI (4226) EcoO109I (4169) pBRforEco (4153 .. 4171) AatII (4115) ZraI (4113) XmnI (3792) Amp-R (3743 .. 3762) NmeAIII (3341) BmrI (3233) AlwNI (2716) PspFI (2608) BseYI (2604) pBR322ori-F (2441 .. 2460) PciI (2300) L4440 (2190 .. 2207) PvuII (15) BsiWI (25) SalI (37) BamHI (43) AvaI - BsoBI - TspMI - XmaI (48) SmaI (50) PacI (58) XcmI (87) Acc65I (132) KpnI (136) AscI - BssHII (343) PsiI (404) BstZ17I (421) pHybLex-R (417 .. 441) I-SceI (567) BglII (579) BstEII (609) BmgBI (662) BseRI (773) MluI (826) NcoI - StyI (966) NruI (1050) Kan-R (1035 .. 1054) EcoNI (1305) AsiSI (1393) PflMI (1656) PmeI (2014) Eco53kI (2021) SacI (2023) EcoRI (2025) EcoRV (2039) SpeI (2049) SfiI (2062) SacII (2069) T7 (2084 .. 2103) HpaI (2121) BspQI - SapI (2184) pFA6a-HBH-kanMX6 4447 bp

Sequence View

PacISalIBamHITspMIBsoBIXmaIAvaISmaIBsiWIPvuII 5ʹ 3ʹ 60 SP6 promoter G SP6 g a a c g c g g c c g c c a g c t g a a g c t t c g t a c g c t g c a g g t c g a c g g a t c c c c g g g t t a a t t a c t t g c g c c g g c g g t c g a c t t c g a a g c a t g c g a c g t c c a g c t g c c t a g g g g c c c a a t t a a t

XcmI 120 1 5 HHHHHH (in frame with 6xHis) AGKAGEGEIP 6xHis a c a g g g g t t c a c a t c a t c a c c a c c a t c a t g c t g g a a a g g c c g g t g a a g g t g a a a t c c c t g t g t c c c c a a g t g t a g t a g t g g t g g t a g t a c g a c c t t t c c g g c c a c t t c c a c t t t a g g g a c

Acc65IKpnI 180 (in frame with 6xHis) APLAGTVSKILVKEGDTVKA c c c c t c t t g c t g g t a c c g t t t c t a a g a t a c t g g t a a a a g a a g g t g a c a c t g t t a a a g c t g g g g g a g a a c g a c c a t g g c a a a g a t t c t a t g a c c a t t t t c t t c c a c t g t g a c a a t t t c g a c

240 (in frame with 6xHis) GQTVLVLEAMKMETEINAPT g t c a a a c a g t t c t g g t g c t g g a g g c t a t g a a a a t g g a g a c a g a a a t t a a c g c t c c t a c t g c a g t t t g t c a a g a c c a c g a c c t c c g a t a c t t t t a c c t c t g t c t t t a a t t g c g a g g a t g a c

300 (in frame with 6xHis) DGKVEKVLVKERDAVQGGQG a c g g a a a a g t t g a a a a g g t g t t a g t t a a g g a a a g a g a t g c t g t t c a a g g t g g t c a a g g t c t g c c t t t t c a a c t t t t c c a c a a t c a a t t c c t t t c t c t a c g a c a a g t t c c a c c a g t t c c a g

BssHIIAscI 360 (in frame with 6xHis) LIKIGV 1 5 HHHHHH * 6xHis t a a t c a a g a t c g g c g t t c a t c a t c a c c a c c a t c a t t a a t g a g g c g c g c c a c t t c t a a a t a a t t a g t t c t a g c c g c a a g t a g t a g t g g t g g t a g t a a t t a c t c c g c g c g g t g a a g a t t t a t

PsiI 420 ADH1 terminator C A C A pHybLex-R a g c g a a t t t c t t a t g a t t t a t g a t t t t t a t t a t t a a a t a a g t t a t a a a a a a a a t a a g t g t t c g c t t a a a g a a t a c t a a a t a c t a a a a a t a a t a a t t t a t t c a a t a t t t t t t t t a t t c a c a

BstZ17I 480 ADH1 terminator T A T G T T T A A A A T T T C A C T G A G pHybLex-R a t a c a a a t t t t a a a g t g a c t c t t a g g t t t t a a a a c g a a a a t t c t t a t t c t t g a g t a a c t c t a t g t t t a a a a t t t c a c t g a g a a t c c a a a a t t t t g c t t t t a a g a a t a a g a a c t c a t t g a g

540 ADH1 terminator t t t c c t g t a g g t c a g g t t g c t t t c t c a g g t a t a g t a t g a g g t c g c t c t t a t t g a c c a c a c a a a g g a c a t c c a g t c c a a c g a a a g a g t c c a t a t c a t a c t c c a g c g a g a a t a a c t g g t g t g

BglIII-SceI 600 ADH1 terminator c t c t a c c g g c a g a t c c g c t a g g g a t a a c a g g g t a a t a t a g a t c t g t t t a g c t t g c c t c g t g a g a t g g c c g t c t a g g c g a t c c c t a t t g t c c c a t t a t a t c t a g a c a a a t c g a a c g g a g c a

BstEII 660 kanMX TEF promoter c c c c g c c g g g t c a c c c g g c c a g c g a c a t g g a g g c c c a g a a t a c c c t c c t t g a c a g t c t t g g g g g c g g c c c a g t g g g c c g g t c g c t g t a c c t c c g g g t c t t a t g g g a g g a a c t g t c a g a a c

BmgBI 720 kanMX TEF promoter a c g t g c g c a g c t c a g g g g c a t g a t g t g a c t g t c g c c c g t a c a t t t a g c c c a t a c a t c c c c t g c a c g c g t c g a g t c c c c g t a c t a c a c t g a c a g c g g g c a t g t a a a t c g g g t a t g t a g g g g

BseRI 780 kanMX TEF promoter a t g t a t a a t c a t t t g c a t c c a t a c a t t t t g a t g g c c g c a c g g c g c g a a g c a a a a a t t a c g t a c a t a t t a g t a a a c g t a g g t a t g t a a a a c t a c c g g c g t g c c g c g c t t c g t t t t t a a t g c

MluI 840 kanMX TEF promoter g c t c c t c g c t g c a g a c c t g c g a g c a g g g a a a c g c t c c c c t c a c a g a c g c g t t g a a t t g t c c g a g g a g c g a c g t c t g g a c g c t c g t c c c t t t g c g a g g g g a g t g t c t g c g c a a c t t a a c a g

900 kanMX TEF promoter c c c a c g c c g c g c c c c t g t a g a g a a a t a t a a a a g g t t a g g a t t t g c c a c t g a g g t t c t t c t g g g t g c g g c g c g g g g a c a t c t c t t t a t a t t t t c c a a t c c t a a a c g g t g a c t c c a a g a a g a

960 kanMX TEF promoter t t c a t a t a c t t c c t t t t a a a a t c t t g c t a g g a t a c a g t t c t c a c a t c a c a t c c g a a c a t a a a g t a t a t g a a g g a a a a t t t t a g a a c g a t c c t a t g t c a a g a g t g t a g t g t a g g c t t g t a t

NcoIStyI 1020 kanMX 1 5 10 15 MGKEKTHVSRPRLNSNMD TEF promoter KanR MGKEKTHVSRPRLNSNMD a a c a a c c a t g g g t a a g g a a a a g a c t c a c g t t t c g a g g c c g c g a t t a a a t t c c a a c a t g g a t t g t t g g t a c c c a t t c c t t t t c t g a g t g c a a a g c t c c g g c g c t a a t t t a a g g t t g t a c c t

NruI 1080 kanMX 20 25 30 35 ADLYGYKWARDNVGQSGATI KanR C C A T A T T T A C C C G A G C G C T A Kan-R ADLYGYKWARDNVGQSGATI t g c t g a t t t a t a t g g g t a t a a a t g g g c t c g c g a t a a t g t c g g g c a a t c a g g t g c g a c a a t a c g a c t a a a t a t a c c c a t a t t t a c c c g a g c g c t a t t a c a g c c c g t t a g t c c a c g c t g t t a

1140 kanMX 40 45 50 55 YRLYGKPDAPELFLKHGKGS KanR YRLYGKPDAPELFLKHGKGS c t a t c g a t t g t a t g g g a a g c c c g a t g c g c c a g a g t t g t t t c t g a a a c a t g g c a a a g g t a g g a t a g c t a a c a t a c c c t t c g g g c t a c g c g g t c t c a a c a a a g a c t t t g t a c c g t t t c c a t c

1200 kanMX 60 65 70 75 VANDVTDEMVRLNWLTEFMP KanR VANDVTDEMVRLNWLTEFMP c g t t g c c a a t g a t g t t a c a g a t g a g a t g g t c a g a c t a a a c t g g c t g a c g g a a t t t a t g c c g c a a c g g t t a c t a c a a t g t c t a c t c t a c c a g t c t g a t t t g a c c g a c t g c c t t a a a t a c g g

1260 kanMX 80 85 90 95 LPTIKHFIRTPDDAWLLTTA KanR LPTIKHFIRTPDDAWLLTTA t c t t c c g a c c a t c a a g c a t t t t a t c c g t a c t c c t g a t g a t g c a t g g t t a c t c a c c a c t g c a g a a g g c t g g t a g t t c g t a a a a t a g g c a t g a g g a c t a c t a c g t a c c a a t g a g t g g t g a c g

EcoNI 1320 kanMX 100 105 110 115 IPGKTAFQVLEEYPDSGENI KanR IPGKTAFQVLEEYPDSGENI g a t c c c c g g c a a a a c a g c a t t c c a g g t a t t a g a a g a a t a t c c t g a t t c a g g t g a a a a t a t c t a g g g g c c g t t t t g t c g t a a g g t c c a t a a t c t t c t t a t a g g a c t a a g t c c a c t t t t a t a

1380 kanMX 120 125 130 135 VDALAVFLRRLHSIPVCNCP KanR VDALAVFLRRLHSIPVCNCP t g t t g a t g c g c t g g c a g t g t t c c t g c g c c g g t t g c a t t c g a t t c c t g t t t g t a a t t g t c c a c a a c t a c g c g a c c g t c a c a a g g a c g c g g c c a a c g t a a g c t a a g g a c a a a c a t t a a c a g g

AsiSI 1440 kanMX 140 145 150 155 FNSDRVFRLAQAQSRMNNGL KanR FNSDRVFRLAQAQSRMNNGL t t t t a a c a g c g a t c g c g t a t t t c g t c t c g c t c a g g c g c a a t c a c g a a t g a a t a a c g g t t t a a a a t t g t c g c t a g c g c a t a a a g c a g a g c g a g t c c g c g t t a g t g c t t a c t t a t t g c c a a a

1500 kanMX 160 165 170 175 VDASDFDDERNGWPVEQVWK KanR VDASDFDDERNGWPVEQVWK g g t t g a t g c g a g t g a t t t t g a t g a c g a g c g t a a t g g c t g g c c t g t t g a a c a a g t c t g g a a c c a a c t a c g c t c a c t a a a a c t a c t g c t c g c a t t a c c g a c c g g a c a a c t t g t t c a g a c c t t

1560 kanMX 180 185 190 195 EMHKLLPFSPDSVVTHGDFS KanR EMHKLLPFSPDSVVTHGDFS a g a a a t g c a t a a g c t t t t g c c a t t c t c a c c g g a t t c a g t c g t c a c t c a t g g t g a t t t c t c t c t t t a c g t a t t c g a a a a c g g t a a g a g t g g c c t a a g t c a g c a g t g a g t a c c a c t a a a g a g

1620 kanMX 200 205 210 215 LDNLIFDEGKLIGCIDVGRV KanR LDNLIFDEGKLIGCIDVGRV a c t t g a t a a c c t t a t t t t t g a c g a g g g g a a a t t a a t a g g t t g t a t t g a t g t t g g a c g a g t t g a a c t a t t g g a a t a a a a a c t g c t c c c c t t t a a t t a t c c a a c a t a a c t a c a a c c t g c t c a

PflMI 1680 kanMX 220 225 230 235 GIADRYQDLAILWNCLGEFS KanR GIADRYQDLAILWNCLGEFS c g g a a t c g c a g a c c g a t a c c a g g a t c t t g c c a t c c t a t g g a a c t g c c t c g g t g a g t t t t c g c c t t a g c g t c t g g c t a t g g t c c t a g a a c g g t a g g a t a c c t t g a c g g a g c c a c t c a a a a g

1740 kanMX 240 245 250 255 PSLQKRLFQKYGIDNPDMNK KanR PSLQKRLFQKYGIDNPDMNK t c c t t c a t t a c a g a a a c g g c t t t t t c a a a a a t a t g g t a t t g a t a a t c c t g a t a t g a a t a a a g g a a g t a a t g t c t t t g c c g a a a a a g t t t t t a t a c c a t a a c t a t t a g g a c t a t a c t t a t t

1800 kanMX 260 265 270 LQFHLMLDEFF* KanR TEF terminator LQFHLMLDEFF* a t t g c a g t t t c a t t t g a t g c t c g a t g a g t t t t t c t a a t c a g t a c t g a c a a t a a a a a g a t t t a a c g t c a a a g t a a a c t a c g a g c t a c t c a a a a a g a t t a g t c a t g a c t g t t a t t t t t c t a a

1860 kanMX TEF terminator c t t g t t t t c a a g a a c t t g t c a t t t g t a t a g t t t t t t t a t a t t g t a g t t g t t c t a t t t t a a g a a c a a a a g t t c t t g a a c a g t a a a c a t a t c a a a a a a a t a t a a c a t c a a c a a g a t a a a a t t

1920 kanMX TEF terminator t c a a a t g t t a g c g t g a t t t a t a t t t t t t t t c g c c t c g a c a t c a t c t g c c c a g a t g c g a a g a g t t t a c a a t c g c a c t a a a t a t a a a a a a a a g c g g a g c t g t a g t a g a c g g g t c t a c g c t t c

1980 kanMX TEF terminator t t a a g t g c g c a g a a a g t a a t a t c a t g c g t c a a t c g t a t g t g a a t g c t g g t c g c t a t a c t g a a t t c a c g c g t c t t t c a t t a t a g t a c g c a g t t a g c a t a c a c t t a c g a c c a g c g a t a t g a c

EcoRVPmeIEco53kISacIEcoRI 2040 c t g t c g a t t c g a t a c t a a c g c c g c c a t c c a g t t t a a a c g a g c t c g a a t t c a t c g a t g a t a g a c a g c t a a g c t a t g a t t g c g g c g g t a g g t c a a a t t t g c t c g a g c t t a a g t a g c t a c t a t

SacIISfiISpeI 2100 T7 promoter G G G A T A T C A C T C A G C A T T7 t c a g a t c c a c t a g t g g c c t a t g c g g c c g c g g a t c t g c c g g t c t c c c t a t a g t g a g t c g t a a g t c t a g g t g a t c a c c g g a t a c g c c g g c g c c t a g a c g g c c a g a g g g a t a t c a c t c a g c a t

HpaI 2160 T7 promoter A A T T7 t t a a t t t c g a t a a g c c a g g t t a a c c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c a a t t a a a g c t a t t c g g t c c a a t t g g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g

BspQISapI 2220 G A G C G A G T G A C T G A G C G A L4440 g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a

2280 c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t

PciI 2340 g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t

2400 ori a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a

2460 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g

2520 ori c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g

2580 ori g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a a t g c t c a c g c t g t a g g t a t c t c a g t c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t t a c g a g t g c g a c a t c c a t a g a g t c a

BseYIPspFI 2640 ori t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g

2700 ori c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c

AlwNI 2760 ori c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t

2820 ori g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g g a c a g t a t t t g g t a t c t g c c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c c t g t c a t a a a c c a t a g a c g

2880 ori g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t

2940 ori a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t

3000 ori g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g

3060 t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t

3120 AmpR a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a

3180 285 280 275 270 *WHKILSAGIEAIQRNREDM AmpR *WHKILSAGIEAIQRNREDM t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t

BmrI 3240 265 260 255 250 TAQSGTTYIVVIRSPKGDPG AmpR TAQSGTTYIVVIRSPKGDPG g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g

3300 245 240 235 230 LAAIIGRSGREGAGSKDAIF AmpR LAAIIGRSGREGAGSKDAIF a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g

NmeAIII 3360 225 220 215 210 WGAPLASRLLPGAVKDAEMW AmpR WGAPLASRLLPGAVKDAEMW c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c

3420 205 200 195 190 DILQQRSALTLLEGTLLKRL AmpR DILQQRSALTLLEGTLLKRL t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g

3480 185 180 175 170 TTAMAVPMTTDREDNPIAEN AmpR TTAMAVPMTTDREDNPIAEN g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g

3540 165 160 155 150 LEPEWRDLRTVHDGMNHLFA AmpR LEPEWRDLRTVHDGMNHLFA a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c

3600 145 140 135 130 TLEKPGGITTLLLNAATNDS AmpR TLEKPGGITTLLLNAATNDS g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g

3660 125 120 115 110 MTIAASCLERVTMGDTLHKE AmpR MTIAASCLERVTMGDTLHKE a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a

3720 105 100 95 90 TVPSYEVLDNQSYHIRRGLQ AmpR TVPSYEVLDNQSYHIRRGLQ g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g

3780 85 80 75 70 EQGADIRSLVAGCLLVKFTS AmpR A T A A T A C C G C G C C A C A T A G C Amp-R EQGADIRSLVAGCLLVKFTS t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g

XmnI 3840 65 60 55 50 MMPFREEPRFSELIKGSNLD AmpR MMPFREEPRFSELIKGSNLD a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g

3900 45 40 35 30 LEIYGVRAGLQDEADKVKVL AmpR LEIYGVRAGLQDEADKVKVL a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g

3960 25 TEPH 20 15 10 signal sequence AFVPLCFAAFFPILAV AmpR TEPHAFVPLCFAAFFPILAV g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t

4020 5 1 signal sequence RFHQISM AmpR AmpR promoter RFHQISM c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a

4080 AmpR promoter t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a

ZraIAatII 4140 AmpR promoter c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t a a g a a a c c a t t a t t a t c a t g a c a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g a t t c t t t g g t a a t a a t a g t a c t g t

EcoO109I 4200 A A T A G G C G T A T C A C G A G G C pBRforEco t t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c c c t t t c g t c t c g c g c g t t t c g g t g a t g a c a a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g g g a a a g c a g a g c g c g c a a a g c c a c t a c t g

PfoI 4260 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a c a g c t t g t c t g t a a g c g g a t c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t g t c g a a c a g a c a t t c g c c t a

4320 g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g t t g g c g g g t g t c g g g g c t g g c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c a a c c g c c c a c a g c c c c g a c c

NdeI 4380 C G G C A T C A G A G C A G A T T G T A pRS-marker c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a c c a t a t g g a c a t a t t g t c g g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g t g g t a t a c c t g t a t a a c a g c

4440 SP6 promoter A T T T A G G T G A SP6 t t a g a a c g c g g c t a c a a t t a a t a c a t a a c c t t a t g t a t c a t a c a c a t a c g a t t t a g g t g a a a t c t t g c g c c g a t g t t a a t t a t g t a t t g g a a t a c a t a g t a t g t g t a t g c t a a a t c c a c t

3ʹ 5ʹ 4447 SP6 promoter C A C T A T A SP6 c a c t a t a g t g a t a t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.