Addgene R777-E272 Hs.RB1-nostop - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: R777-E272 Hs.RB1-nostop


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AclI (5342) rrnB T2 terminator BspQI - SapI (5089) L4440 (5070 .. 5087) DrdI (4870) pBR322ori-F (4817 .. 4836) BssSI - BssSαI (4799) PaeR7I - PspXI - XhoI (4238) BsaAI (4199) BssHII (4166) NaeI (4093) NgoMIV (4091) BglI (4074) BtgZI (4000) PvuI (3823) BlpI (3751) MslI (3714) BstEII (3602) HindIII (3073) SphI (3071) BsmI (2920) M13/pUC Forward (23 .. 45) M13 Forward (37 .. 54) AhdI (50) Eco53kI (67) BanII - SacI (69) RsrII (71) BamHI (78) EagI (211) SacII (238) Bsu36I (271) EcoRV (727) EcoRI (1085) BsgI (1388) AccI (1427) BstZ17I (1428) MscI (1671) NcoI (1728) BsaBI (1999) BbvCI - Bpu10I (2084) XmnI (2188) BstAPI (2246) SspI (2486) PfoI * (2582) MfeI (2686) R777-E272 Hs.RB1-nostop 5477 bp

Sequence View

AhdI 60 M13 fwd C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward t t a a c g c t a g c a t g g a t g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t c t t a a a a a t t g c g a t c g t a c c t a c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a g a a t t t

BamHIEco53kIBanIISacIRsrII 120 attL1 a t t c g a g c t c g g t c c g a g g a t c c g a a a t a a t g a t t t t a t t t t g a c t g a t a g t g a c c t g t t t a a g c t c g a g c c a g g c t c c t a g g c t t t a t t a c t a a a a t a a a a c t g a c t a t c a c t g g a c a a

180 attL1 MPTLYKKV c g t t g c a a c a a a t t g a t a a g c a a t g c t t t t t t a t a a t g c c a a c t t t g t a c a a a a a a g t t g g c a a c g t t g t t t a a c t a t t c g t t a c g a a a a a a t a t t a c g g t t g a a a c a t g t t t t t t c a a c

SacIIEagI 240 attL1 GTMPPKTPRKTAATAAAAAA g c a c c a t g c c g c c c a a a a c c c c c c g a a a a a c g g c c g c c a c c g c c g c c g c t g c c g c c g c g g c g t g g t a c g g c g g g t t t t g g g g g g c t t t t t g c c g g c g g t g g c g g c g g c g a c g g c g g c g c c

Bsu36I 300 EPPAPPPPPPPEEDPEQDSG a a c c c c c g g c a c c g c c g c c g c c g c c c c c t c c t g a g g a g g a c c c a g a g c a g g a c a g c g g c c t t g g g g g c c g t g g c g g c g g c g g c g g g g g a g g a c t c c t c c t g g g t c t c g t c c t g t c g c c g g

360 PEDLPLVRLEFEETEEPDFT c g g a g g a c c t g c c t c t c g t c a g g c t t g a g t t t g a a g a a a c a g a a g a a c c t g a t t t t a c t g g c c t c c t g g a c g g a g a g c a g t c c g a a c t c a a a c t t c t t t g t c t t c t t g g a c t a a a a t g a c

420 ALCQKLKIPDHVRERAWLTW c a t t a t g t c a g a a a t t a a a g a t a c c a g a t c a t g t c a g a g a g a g a g c t t g g t t a a c t t g g g g t a a t a c a g t c t t t a a t t t c t a t g g t c t a g t a c a g t c t c t c t c t c g a a c c a a t t g a a c c c

480 EKVSSVDGVLGGYIQKKKEL a g a a a g t t t c a t c t g t g g a t g g a g t a t t g g g a g g t t a t a t t c a a a a g a a a a a g g a a c t g t t c t t t c a a a g t a g a c a c c t a c c t c a t a a c c c t c c a a t a t a a g t t t t c t t t t t c c t t g a c a

540 WGICIFIAAVDLDEMSFTFT g g g g a a t c t g t a t c t t t a t t g c a g c a g t t g a c c t a g a t g a g a t g t c g t t c a c t t t t a c t g c c c c t t a g a c a t a g a a a t a a c g t c g t c a a c t g g a t c t a c t c t a c a g c a a g t g a a a a t g a c

600 ELQKNIEISVHKFFNLLKEI a g c t a c a g a a a a a c a t a g a a a t c a g t g t c c a t a a a t t c t t t a a c t t a c t a a a a g a a a t t g t c g a t g t c t t t t t g t a t c t t t a g t c a c a g g t a t t t a a g a a a t t g a a t g a t t t t c t t t a a c

660 DTSTKVDNAMSRLLKKYDVL a t a c c a g t a c c a a a g t t g a t a a t g c t a t g t c a a g a c t g t t g a a g a a g t a t g a t g t a t t g t t a t g g t c a t g g t t t c a a c t a t t a c g a t a c a g t t c t g a c a a c t t c t t c a t a c t a c a t a a c a

720 FALFSKLERTCELIYLTQPS t t g c a c t c t t c a g c a a a t t g g a a a g g a c a t g t g a a c t t a t a t a t t t g a c a c a a c c c a g c a a a c g t g a g a a g t c g t t t a a c c t t t c c t g t a c a c t t g a a t a t a t a a a c t g t g t t g g g t c g t

EcoRV 780 SSISTEINSALVLKVSWITF g t t c g a t a t c t a c t g a a a t a a a t t c t g c a t t g g t g c t a a a a g t t t c t t g g a t c a c a t t t t c a a g c t a t a g a t g a c t t t a t t t a a g a c g t a a c c a c g a t t t t c a a a g a a c c t a g t g t a a a a

840 LLAKGEVLQMEDDLVISFQL t a t t a g c t a a a g g g g a a g t a t t a c a a a t g g a a g a t g a t c t g g t g a t t t c a t t t c a g t t a a a t a a t c g a t t t c c c c t t c a t a a t g t t t a c c t t c t a c t a g a c c a c t a a a g t a a a g t c a a t t

900 MLCVLDYFIKLSPPMLLKEP t g c t a t g t g t c c t t g a c t a t t t t a t t a a a c t c t c a c c t c c c a t g t t g c t c a a a g a a c c a t a c g a t a c a c a g g a a c t g a t a a a a t a a t t t g a g a g t g g a g g g t a c a a c g a g t t t c t t g g t a

960 YKTAVIPINGSPRTPRRGQN a t a a a a c a g c t g t t a t a c c c a t t a a t g g t t c a c c t c g a a c a c c c a g g c g a g g t c a g a a c a t a t t t t g t c g a c a a t a t g g g t a a t t a c c a a g t g g a g c t t g t g g g t c c g c t c c a g t c t t g t

1020 RSARIAKQLENDTRIIEVLC g g a g t g c a c g g a t a g c a a a a c a a c t a g a a a a t g a t a c a a g a a t t a t t g a a g t t c t c t g t a c c t c a c g t g c c t a t c g t t t t g t t g a t c t t t t a c t a t g t t c t t a a t a a c t t c a a g a g a c a t

1080 KEHECNIDEVKNVYFKNFIP a a g a a c a t g a a t g t a a t a t a g a t g a g g t g a a a a a t g t t t a t t t c a a a a a t t t t a t a c c t t t t c t t g t a c t t a c a t t a t a t c t a c t c c a c t t t t t a c a a a t a a a g t t t t t a a a a t a t g g a a

EcoRI 1140 FMNSLGLVTSNGLPEVENLS t t a t g a a t t c t c t t g g a c t t g t a a c a t c t a a t g g a c t t c c a g a g g t t g a a a a t c t t t c t a a a t a c t t a a g a g a a c c t g a a c a t t g t a g a t t a c c t g a a g g t c t c c a a c t t t t a g a a a g a t

1200 KRYEEIYLKNKDLDARLFLD a a c g a t a c g a a g a a a t t t a t c t t a a a a a t a a a g a t c t a g a t g c a a g a t t a t t t t t g g a t c t t g c t a t g c t t c t t t a a a t a g a a t t t t t a t t t c t a g a t c t a c g t t c t a a t a a a a a c c t a g

1260 HDKTLQTDSIDSFETQRTPR a t g a t a a a a c t c t t c a g a c t g a t t c t a t a g a c a g t t t t g a a a c a c a g a g a a c a c c a c g a a t a c t a t t t t g a g a a g t c t g a c t a a g a t a t c t g t c a a a a c t t t g t g t c t c t t g t g g t g c t t

1320 KSNLDEEVNVIPPHTPVRTV a a a g t a a c c t t g a t g a a g a g g t g a a t g t a a t t c c t c c a c a c a c t c c a g t t a g g a c t g t t a t t t c a t t g g a a c t a c t t c t c c a c t t a c a t t a a g g a g g t g t g t g a g g t c a a t c c t g a c a a t

1380 MNTIQQLMMILNSASDQPSE t g a a c a c t a t c c a a c a a t t a a t g a t g a t t t t a a a t t c a g c a a g t g a t c a a c c t t c a g a a a a c t t g t g a t a g g t t g t t a a t t a c t a c t a a a a t t t a a g t c g t t c a c t a g t t g g a a g t c t t t

AccIBstZ17IBsgI 1440 NLISYFNNCTVNPKESILKR a t c t g a t t t c c t a t t t t a a c a a c t g c a c a g t g a a t c c a a a a g a a a g t a t a c t g a a a a g a g t a g a c t a a a g g a t a a a a t t g t t g a c g t g t c a c t t a g g t t t t c t t t c a t a t g a c t t t t c t c

1500 VKDIGYIFKEKFAKAVGQGC t g a a g g a t a t a g g a t a c a t c t t t a a a g a g a a a t t t g c t a a a g c t g t g g g a c a g g g t t g t g a c t t c c t a t a t c c t a t g t a g a a a t t t c t c t t t a a a c g a t t t c g a c a c c c t g t c c c a a c a c

1560 VEIGSQRYKLGVRLYYRVME t c g a a a t t g g a t c a c a g c g a t a c a a a c t t g g a g t t c g c t t g t a t t a c c g a g t a a t g g a a t a g c t t t a a c c t a g t g t c g c t a t g t t t g a a c c t c a a g c g a a c a t a a t g g c t c a t t a c c t t a

1620 SMLKSEEERLSIQNFSKLLN c c a t g c t t a a a t c a g a a g a a g a a c g a t t a t c c a t t c a a a a t t t t a g c a a a c t t c t g a a t g g g t a c g a a t t t a g t c t t c t t c t t g c t a a t a g g t a a g t t t t a a a a t c g t t t g a a g a c t t a c

MscI 1680 DNIFHMSLLACALEVVMATY a c a a c a t t t t t c a t a t g t c t t t a t t g g c g t g c g c t c t t g a g g t t g t a a t g g c c a c a t a t a t g t t g t a a a a a g t a t a c a g a a a t a a c c g c a c g c g a g a a c t c c a a c a t t a c c g g t g t a t a t

NcoI 1740 SRSTSQNLDSGTDLSFPWIL g c a g a a g t a c a t c t c a g a a t c t t g a t t c t g g a a c a g a t t t g t c t t t c c c a t g g a t t c t g a c g t c t t c a t g t a g a g t c t t a g a a c t a a g a c c t t g t c t a a a c a g a a a g g g t a c c t a a g a c t

1800 NVLNLKAFDFYKVIESFIKA a t g t g c t t a a t t t a a a a g c c t t t g a t t t t t a c a a a g t g a t c g a a a g t t t t a t c a a a g c a g t a c a c g a a t t a a a t t t t c g g a a a c t a a a a a t g t t t c a c t a g c t t t c a a a a t a g t t t c g t c

1860 EGNLTREMIKHLERCEHRIM a a g g c a a c t t g a c a a g a g a a a t g a t a a a a c a t t t a g a a c g a t g t g a a c a t c g a a t c a t g g t t c c g t t g a a c t g t t c t c t t t a c t a t t t t g t a a a t c t t g c t a c a c t t g t a g c t t a g t a c c

1920 ESLAWLSDSPLFDLIKQSKD a a t c c c t t g c a t g g c t c t c a g a t t c a c c t t t a t t t g a t c t t a t t a a a c a a t c a a a g g a c c t t a g g g a a c g t a c c g a g a g t c t a a g t g g a a a t a a a c t a g a a t a a t t t g t t a g t t t c c t g g

1980 REGPTDHLESACPLNLPLQN g a g a a g g a c c a a c t g a t c a c c t t g a a t c t g c t t g t c c t c t t a a t c t t c c t c t c c a g a a t a c t c t t c c t g g t t g a c t a g t g g a a c t t a g a c g a a c a g g a g a a t t a g a a g g a g a g g t c t t a t

BsaBI 2040 NHTAADMYLSPVRSPKKKGS a t c a c a c t g c a g c a g a t a t g t a t c t t t c t c c t g t a a g a t c t c c a a a g a a a a a a g g t t c a a t a g t g t g a c g t c g t c t a t a c a t a g a a a g a g g a c a t t c t a g a g g t t t c t t t t t t c c a a g t t

Bpu10IBbvCI 2100 TTRVNSTANAETQATSAFQT c t a c g c g t g t a a a t t c t a c t g c a a a t g c a g a g a c a c a a g c a a c c t c a g c c t t c c a g a c c c g a t g c g c a c a t t t a a g a t g a c g t t t a c g t c t c t g t g t t c g t t g g a g t c g g a a g g t c t g g g

2160 QKPLKSTSLSLFYKKVYRLA a g a a g c c a t t g a a a t c t a c c t c t c t t t c a c t g t t t t a t a a a a a a g t g t a t c g g c t a g c c t t c t t c g g t a a c t t t a g a t g g a g a g a a a g t g a c a a a a t a t t t t t t c a c a t a g c c g a t c g g a

XmnI 2220 YLRLNTLCERLLSEHPELEH a t c t c c g g c t a a a t a c a c t t t g t g a a c g c c t t c t g t c t g a g c a c c c a g a a t t a g a a c a t a t a g a g g c c g a t t t a t g t g a a a c a c t t g c g g a a g a c a g a c t c g t g g g t c t t a a t c t t g t a t

BstAPI 2280 IIWTLFQHTLQNEYELMRDR t c a t c t g g a c c c t t t t c c a g c a c a c c c t g c a g a a t g a g t a t g a a c t c a t g a g a g a c a g g c a g t a g a c c t g g g a a a a g g t c g t g t g g g a c g t c t t a c t c a t a c t t g a g t a c t c t c t g t c c g

2340 HLDQIMMCSMYGICKVKNID a t t t g g a c c a a a t t a t g a t g t g t t c c a t g t a t g g c a t a t g c a a a g t g a a g a a t a t a g a c c t a a a c c t g g t t t a a t a c t a c a c a a g g t a c a t a c c g t a t a c g t t t c a c t t c t t a t a t c t g g

2400 LKFKIIVTAYKDLPHAVQET t t a a a t t c a a a a t c a t t g t a a c a g c a t a c a a g g a t c t t c c t c a t g c t g t t c a g g a g a c a t a a t t t a a g t t t t a g t a a c a t t g t c g t a t g t t c c t a g a a g g a g t a c g a c a a g t c c t c t g t a

2460 FKRVLIKEEEYDSIIVFYNS t c a a a c g t g t t t t g a t c a a a g a a g a g g a g t a t g a t t c t a t t a t a g t a t t c t a t a a c t c g g a g t t t g c a c a a a a c t a g t t t c t t c t c c t c a t a c t a a g a t a a t a t c a t a a g a t a t t g a g c c

SspI 2520 VFMQRLKTNILQYASTRPPT t c t t c a t g c a g a g a c t g a a a a c a a a t a t t t t g c a g t a t g c t t c c a c c a g g c c c c c t a c c t a g a a g t a c g t c t c t g a c t t t t g t t t a t a a a a c g t c a t a c g a a g g t g g t c c g g g g g a t g g a

2580 LSPIPHIPRSPYKFPSSPLR t g t c a c c a a t a c c t c a c a t t c c t c g a a g c c c t t a c a a g t t t c c t a g t t c a c c c t t a c g g a a c a g t g g t t a t g g a g t g t a a g g a g c t t c g g g a a t g t t c a a a g g a t c a a g t g g g a a t g c c t

PfoI* 2640 IPGGNIYISPLKSPYKISEG t t c c t g g a g g g a a c a t c t a t a t t t c a c c c c t g a a g a g t c c a t a t a a a a t t t c a g a a g g t c a a g g a c c t c c c t t g t a g a t a t a a a g t g g g g a c t t c t c a g g t a t a t t t t a a a g t c t t c c a g

MfeI 2700 LPTPTKMTPRSRILVSIGES t g c c a a c a c c a a c a a a a a t g a c t c c a a g a t c a a g a a t c t t a g t a t c a a t t g g t g a a t c a t a c g g t t g t g g t t g t t t t t a c t g a g g t t c t a g t t c t t a g a a t c a t a g t t a a c c a c t t a g t a

2760 FGTSEKFQKINQMVCNSDRV t c g g g a c t t c t g a g a a g t t c c a g a a a a t a a a t c a g a t g g t a t g t a a c a g c g a c c g t g t g c a g c c c t g a a g a c t c t t c a a g g t c t t t t a t t t a g t c t a c c a t a c a t t g t c g c t g g c a c a c g

2820 LKRSAEGSNPPKPLKKLRFD t c a a a a g a a g t g c t g a a g g a a g c a a c c c t c c t a a a c c a c t g a a a a a a c t a c g c t t t g a t a a g t t t t c t t c a c g a c t t c c t t c g t t g g g a g g a t t t g g t g a c t t t t t t g a t g c g a a a c t a t

2880 IEGSDEADGSKHLPGESKFQ t t g a a g g a t c a g a t g a a g c a g a t g g a a g t a a a c a t c t c c c a g g a g a g t c c a a a t t t c a g c a a c t t c c t a g t c t a c t t c g t c t a c c t t c a t t t g t a g a g g g t c c t c t c a g g t t t a a a g t c g

BsmI 2940 QKLAEMTSTRTRMQKQKMND a g a a a c t g g c a g a a a t g a c t t c t a c t c g a a c a c g a a t g c a a a a g c a g a a a a t g a a t g a t a t c t t t g a c c g t c t t t a c t g a a g a t g a g c t t g t g c t t a c g t t t t c g t c t t t t a c t t a c t a t

3000 attL2 SMDTSNKEEKSTFLYKVGII g c a t g g a t a c c t c a a a c a a g g a a g a g a a a t c a a c t t t c t t g t a c a a a g t t g g c a t t a t a g c g t a c c t a t g g a g t t t g t t c c t t c t c t t t a g t t g a a a g a a c a t g t t t c a a c c g t a a t a t c

3060 attL2 GKHCLSICCNEQVTISQNKI g a a a g c a t t g c t t a t c a a t t t g t t g c a a c g a a c a g g t c a c t a t c a g t c a a a a t a a a a t c a c t t t c g t a a c g a a t a g t t a a a c a a c g t t g c t t g t c c a g t g a t a g t c a g t t t t a t t t t a g t

SphIHindIII 3120 attL2 IICMQA* t t a t t t g c a t g c a a g c t t a g a t c c t c t a g a c c a g c c a g g a c a g a a a t g c c t c g a c t t c g c a a t a a a c g t a c g t t c g a a t c t a g g a g a t c t g g t c g g t c c t g t c t t t a c g g a g c t g a a g c g

3180 t g c t a c c c a a g g t t g c c g g g t g a c g c a c a c c g t g g a a a c g g a t g a a g g c a c g a a c c c a g t a c g a t g g g t t c c a a c g g c c c a c t g c g t g t g g c a c c t t t g c c t a c t t c c g t g c t t g g g t c a

3240 MRSRN g g a c a t a a g c c t g t t c g g t t c g t a a g c t g t a a t g c a a g t a g c g t a t g c g c t c a c g c a a c t c c t g t a t t c g g a c a a g c c a a g c a t t c g a c a t t a c g t t c a t c g c a t a c g c g a g t g c g t t g a

3300 WSRTLTERSGGNGAVAVFMA g g t c c a g a a c c t t g a c c g a a c g c a g c g g t g g t a a c g g c g c a g t g g c g g t t t t c a t g g c t t c c a g g t c t t g g a a c t g g c t t g c g t c g c c a c c a t t g c c g c g t c a c c g c c a a a a g t a c c g a a

3360 CYDCFFGVQSMPRASKQQAR g t t a t g a c t g t t t t t t t g g g g t a c a g t c t a t g c c t c g g g c a t c c a a g c a g c a a g c g c g t t c a a t a c t g a c a a a a a a a c c c c a t g t c a g a t a c g g a g c c c g t a g g t t c g t c g t t c g c g c a a

3420 YAVGRCLMLWSSNDVTQQGS a c g c c g t g g g t c g a t g t t t g a t g t t a t g g a g c a g c a a c g a t g t t a c g c a g c a g g g c a g t c t g c g g c a c c c a g c t a c a a a c t a c a a t a c c t c g t c g t t g c t a c a a t g c g t c g t c c c g t c a g

3480 1 5 10 MREAVIAEVSTQ SmR RPKTKLNIMREAVIAEVSTQ g c c c t a a a a c a a a g t t a a a c a t t a t g a g g g a a g c g g t g a t c g c c g a a g t a t c g a c t c a a c c g g g a t t t t g t t t c a a t t t g t a a t a c t c c c t t c g c c a c t a g c g g c t t c a t a g c t g a g t t g

3540 15 20 25 30 LSEVVGVIERHLEPTLLAVH SmR LSEVVGVIERHLEPTLLAVH t a t c a g a g g t a g t t g g c g t c a t c g a g c g c c a t c t c g a a c c g a c g t t g c t g g c c g t a c a t t a t a g t c t c c a t c a a c c g c a g t a g c t c g c g g t a g a g c t t g g c t g c a a c g a c c g g c a t g t a a

3600 35 40 45 50 LYGSAVDGGLKPHSDIDLLV SmR LYGSAVDGGLKPHSDIDLLV t g t a c g g c t c c g c a g t g g a t g g c g g c c t g a a g c c a c a c a g t g a t a t t g a t t t g c t g g t t a a c a t g c c g a g g c g t c a c c t a c c g c c g g a c t t c g g t g t g t c a c t a t a a c t a a a c g a c c a a t

BstEII 3660 55 60 65 70 TVTVRLDETTRRALINDLLE SmR TVTVRLDETTRRALINDLLE c g g t g a c c g t a a g g c t t g a t g a a a c a a c g c g g c g a g c t t t g a t c a a c g a c c t t t t g g a a a g c c a c t g g c a t t c c g a a c t a c t t t g t t g c g c c g c t c g a a a c t a g t t g c t g g a a a a c c t t t

MslI 3720 75 80 85 90 TSASPGESEILRAVEVTIVV SmR TSASPGESEILRAVEVTIVV c t t c g g c t t c c c c t g g a g a g a g c g a g a t t c t c c g c g c t g t a g a a g t c a c c a t t g t t g t g c g a a g c c g a a g g g g a c c t c t c t c g c t c t a a g a g g c g c g a c a t c t t c a g t g g t a a c a a c a c g

BlpI 3780 95 100 105 110 HDDIIPWRYPAKRELQFGEW SmR HDDIIPWRYPAKRELQFGEW a c g a c g a c a t c a t t c c g t g g c g t t a t c c a g c t a a g c g c g a a c t g c a a t t t g g a g a a t g g c t g c t g c t g t a g t a a g g c a c c g c a a t a g g t c g a t t c g c g c t t g a c g t t a a a c c t c t t a c c g

PvuI 3840 115 120 125 130 QRNDILAGIFEPATIDIDLA SmR QRNDILAGIFEPATIDIDLA a g c g c a a t g a c a t t c t t g c a g g t a t c t t c g a g c c a g c c a c g a t c g a c a t t g a t c t g g c t a t c g c g t t a c t g t a a g a a c g t c c a t a g a a g c t c g g t c g g t g c t a g c t g t a a c t a g a c c g a t

3900 135 140 145 150 ILLTKAREHSVALVGPAAEE SmR ILLTKAREHSVALVGPAAEE t c t t g c t g a c a a a a g c a a g a g a a c a t a g c g t t g c c t t g g t a g g t c c a g c g g c g g a g g a a c a g a a c g a c t g t t t t c g t t c t c t t g t a t c g c a a c g g a a c c a t c c a g g t c g c c g c c t c c t t g

3960 155 160 165 170 LFDPVPEQDLFEALNETLTL SmR LFDPVPEQDLFEALNETLTL t c t t t g a t c c g g t t c c t g a a c a g g a t c t a t t t g a g g c g c t a a a t g a a a c c t t a a c g c t a t a g a a a c t a g g c c a a g g a c t t g t c c t a g a t a a a c t c c g c g a t t t a c t t t g g a a t t g c g a t a

BtgZI 4020 175 180 185 190 WNSPPDWAGDERNVVLTLSR SmR WNSPPDWAGDERNVVLTLSR g g a a c t c g c c g c c c g a c t g g g c t g g c g a t g a g c g a a a t g t a g t g c t t a c g t t g t c c c g c a c c t t g a g c g g c g g g c t g a c c c g a c c g c t a c t c g c t t t a c a t c a c g a a t g c a a c a g g g c g t

BglI 4080 195 200 205 210 IWYSAVTGKIAPKDVAADWA SmR IWYSAVTGKIAPKDVAADWA t t t g g t a c a g c g c a g t a a c c g g c a a a a t c g c g c c g a a g g a t g t c g c t g c c g a c t g g g c a a a a a c c a t g t c g c g t c a t t g g c c g t t t t a g c g c g g c t t c c t a c a g c g a c g g c t g a c c c g t t

NgoMIVNaeI 4140 215 220 225 230 MERLPAQYQPVILEARQAYL SmR MERLPAQYQPVILEARQAYL t g g a g c g c c t g c c g g c c c a g t a t c a g c c c g t c a t a c t t g a a g c t a g a c a g g c t t a t c t t g a c c t c g c g g a c g g c c g g g t c a t a g t c g g g c a g t a t g a a c t t c g a t c t g t c c g a a t a g a a c

BsaAIBssHII 4200 235 240 245 250 GQEEDRLASRADQLEEFVHY SmR GQEEDRLASRADQLEEFVHY g a c a a g a a g a a g a t c g c t t g g c c t c g c g c g c a g a t c a g t t g g a a g a a t t t g t c c a c t a c g c t g t t c t t c t t c t a g c g a a c c g g a g c g c g c g t c t a g t c a a c c t t c t t a a a c a g g t g a t g c

PaeR7IPspXIXhoI 4260 255 260 VKGEITKVVGK* SmR VKGEITKVVGK* t g a a a g g c g a g a t c a c c a a g g t a g t c g g c a a a t a a c c c t c g a g c c a c c c a t g a c c a a a a t a c t t t c c g c t c t a g t g g t t c c a t c a g c c g t t t a t t g g g a g c t c g g t g g g t a c t g g t t t t a

4320 c c c t t a a c g t g a g t t a c g c g t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g g g a a t t g c a c t c a a t g c g c a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c

4380 ori g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g

4440 ori c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t

4500 ori c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g

4560 ori a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c

4620 ori t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g

4680 ori c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g

4740 ori g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c a t t g a g a a a g c g c c a c g c t t c c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g t a a c t c t t t c g c g g t g c g a a g

BssSαIBssSI 4800 ori c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t

4860 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g

DrdI 4920 ori t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c

4980 c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a

5040 t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t

BspQISapI 5100 A G C G A G T C A G T G A G C G A G L4440 c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g

5160 g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c

5220 a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t a c g c g t a c c g c t a t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a t g c g c a t g g c g a t

5280 rrnB T2 terminator g c c a g g a a g a g t t t g t a g a a a c g c a a a a a g g c c a t c c g t c a g g a t g g c c t t c t g c t t a g t c g g t c c t t c t c a a a c a t c t t t g c g t t t t t c c g g t a g g c a g t c c t a c c g g a a g a c g a a t c a

5340 t t g a t g c c t g g c a g t t t a t g g c g g g c g t c c t g c c c g c c a c c c t c c g g g c c g t t g c t t c a c a a c t a c g g a c c g t c a a a t a c c g c c c g c a g g a c g g g c g g t g g g a g g c c c g g c a a c g a a g t g

AclI 5400 rrnB T1 terminator a a c g t t c a a a t c c g c t c c c g g c g g a t t t g t c c t a c t c a g g a g a g c g t t c a c c g a c a a a c a t t g c a a g t t t a g g c g a g g g c c g c c t a a a c a g g a t g a g t c c t c t c g c a a g t g g c t g t t t g t

5460 rrnB T1 terminator a c a g a t a a a a c g a a a g g c c c a g t c t t c c g a c t g a g c c t t t c g t t t t a t t t g a t g c c t g g c t g t c t a t t t t g c t t t c c g g g t c a g a a g g c t g a c t c g g a a a g c a a a a t a a a c t a c g g a c c g

5477 a g t t c c c t a c t c t c g c g t c a a g g g a t g a g a g c g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.