Addgene R777-E236 Hs.RASAL2-nostop - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: R777-E236 Hs.RASAL2-nostop


Map View

rrnB T1 terminator AclI (6398) BspQI - SapI (6145) L4440 (6126 .. 6143) PciI (6028) pBR322ori-F (5873 .. 5892) PaeR7I - PspXI - XhoI (5294) PvuI (4879) BclI * (4696) SnaBI (3291) HpaI (2) M13/pUC Forward (23 .. 45) M13 Forward (37 .. 54) M13 fwd AhdI (50) RsrII (71) PsiI (153) BspEI (204) SacII (217) PflMI (306) SgrAI (318) Bsu36I (570) KasI (627) NarI (628) SfoI (629) PluTI (631) Acc65I (644) KpnI (648) AccI (668) BsaI (865) AflII (1022) MfeI (1115) AfeI (2265) BglII (2278) NmeAIII (2363) PstI (2548) PfoI * (2743) NsiI (2777) StuI (3000) Bpu10I (3027) R777-E236 Hs.RASAL2-nostop 6533 bp

Sequence View

AhdIHpaI 5ʹ 3ʹ 60 M13 fwd C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward t t a a c g c t a g c a t g g a t g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t c t t a a a a a t t g c g a t c g t a c c t a c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a g a a t t t

RsrII 120 attL1 a t t c g a g c t c g g t c c g a g g a t c c g a a a t a a t g a t t t t a t t t t g a c t g a t a g t g a c c t g t t t a a g c t c g a g c c a g g c t c c t a g g c t t t a t t a c t a a a a t a a a a c t g a c t a t c a c t g g a c a a

PsiI 180 attL1 MLFYNANFVQKSW MPTLYKKV c g t t g c a a c a a a t t g a t a a g c a a t g c t t t t t t a t a a t g c c a a c t t t g t a c a a a a a a g t t g g c a a c g t t g t t t a a c t a t t c g t t a c g a a a a a a t a t t a c g g t t g a a a c a t g t t t t t t c a a c

SacIIBspEI 240 attL1 HHGALSVVRRSRGGAVLAGD GTMELSPSSGGAAEALSWPE g c a c c a t g g a g c t c t c t c c g t c g t c c g g a g g a g c c g c g g a g g c g c t g t c c t g g c c g g a g a c g t g g t a c c t c g a g a g a g g c a g c a g g c c t c c t c g g c g c c t c c g c g a c a g g a c c g g c c t c t

300 VPGAGVRLAAAPGGPGRGCP MFPALESDSPLPPEDLDAVV t g t t c c c g g c g c t g g a g t c c g a c t c g c c g c t g c c c c c g g a g g a c c t g g a c g c g g t t g t c c a c a a g g g c c g c g a c c t c a g g c t g a g c g g c g a c g g g g g c c t c c t g g a c c t g c g c c a a c a g g

SgrAIPflMI 360 SQWSRRRWHVGSDPSGVRVP PVSGAVAGGMLDRILLESVC c a g t c a g t g g a g c c g t c g c c g g t g g c a t g t t g g a t c g g a t c c t t c t g g a g t c c g t g t g c c g t c a g t c a c c t c g g c a g c g g c c a c c g t a c a a c c t a g c c t a g g a a g a c c t c a g g c a c a c g g

420 ATELGPGVRCERTTHPPSVL QQQSWVRVYDVKGPPTHRLS a g c a a c a g a g c t g g g t c c g g g t g t a c g a t g t g a a a g g a c c a c c c a c c c a c c g t c t g t c t t t c g t t g t c t c g a c c c a g g c c c a c a t g c t a c a c t t t c c t g g t g g g t g g g t g g c a g a c a g a a

480 WSVTLHRDNNVGAEVLHPHR CGQSPYTETTTWERKYCILT g t g g t c a g t c a c c c t a c a c c g a g a c a a c a a c g t g g g a g c g g a a g t a t t g c a t c c t c a c a g c a c c a g t c a g t g g g a t g t g g c t c t g t t g t t g c a c c c t c g c c t t c a t a a c g t a g g a g t g t c

540 QPVGIAQQGEGDTCGRGTGA DSQLVLLNKEKEIPVEGGQE a c a g c c a g t t g g t a t t g c t c a a c a a g g a g a a g g a g a t a c c t g t g g a a g g g g g a c a g g a g c t g t c g g t c a a c c a t a a c g a g t t g t t c c t c t t c c t c t a t g g a c a c c t t c c c c c t g t c c t c g

Bsu36I 600 ADRFHQRAMPEENCQCPFRG QQTDSTKGRCLRRTVSVPSE a g c a g a c a g a t t c c a c c a a a g g g c g a t g c c t g a g g a g a a c t g t c a g t g t c c c t t c c g a g g t c g t c t g t c t a a g g t g g t t t c c c g c t a c g g a c t c c t c t t g a c a g t c a c a g g g a a g g c t c c

Acc65IKpnIKasINarISfoIPluTI 660 SVSRVPTRGRH* GQFPEYPPEGATKLEVPAER g t c a g t t t c c c g a g t a c c c a c c a g a g g g c g c c a c t a a a c t g g a g g t a c c a g c a g a a a g g t c a g t c a a a g g g c t c a t g g g t g g t c t c c c g c g g t g a t t t g a c c t c c a t g g t c g t c t t t c c a

AccI 720 SPRRRSISGTSTSEKPNSMD c c c c t c g t a g a c g g a g t a t c t c a g g g a c c a g t a c a t c a g a g a a a c c c a a c t c c a t g g a c a g g g g a g c a t c t g c c t c a t a g a g t c c c t g g t c a t g t a g t c t c t t t g g g t t g a g g t a c c t g t

780 TANTSPFKVPGFFSKRLKGS c t g c a a a t a c c t c a c c c t t c a a a g t a c c a g g a t t c t t c a g c a a g c g c c t g a a a g g c t c c a g a c g t t t a t g g a g t g g g a a g t t t c a t g g t c c t a a g a a g t c g t t c g c g g a c t t t c c g a g g t

840 IKRTKSQSKLDRNTSFRLPS t c a a g a g g a c c a a a a g c c a g t c a a a g c t t g a c a g a a a c a c g a g c t t t c g g c t t c c a t c c c a g t t c t c c t g g t t t t c g g t c a g t t t c g a a c t g t c t t t g t g c t c g a a a g c c g a a g g t a g g g

BsaI 900 LRSTDDRSRGLPKLKESRSH t t c g c a g t a c a g a t g a c a g g t c t c g t g g g c t g c c t a a a c t a a a a g a g t c a c g t t c c c a t g a a g c g t c a t g t c t a c t g t c c a g a g c a c c c g a c g g a t t t g a t t t t c t c a g t g c a a g g g t a c

960 ESLLSPCSTVECLDLGRGEP a a t c c t t g c t g a g c c c a t g c a g c a c a g t g g a a t g t c t g g a t c t t g g t a g a g g g g a a c c t g t t a g g a a c g a c t c g g g t a c g t c g t g t c a c c t t a c a g a c c t a g a a c c a t c t c c c c t t g g a c

1020 VSVKPLHSSILGQDFCFEVT t a t c a g t g a a a c c a c t t c a t a g t a g c a t c c t t g g a c a a g a c t t c t g c t t t g a g g t t a c c t a t a g t c a c t t t g g t g a a g t a t c a t c g t a g g a a c c t g t t c t g a a g a c g a a a c t c c a a t g g a

AflII 1080 YLSGSKCFSCNSASERDKWM a c t t a a g t g g a a g t a a a t g c t t c a g c t g t a a t t c t g c t t c t g a g a g a g a c a a g t g g a t g g t g a a t t c a c c t t c a t t t a c g a a g t c g a c a t t a a g a c g a a g a c t c t c t c t g t t c a c c t a c c

MfeI 1140 ENLRRTVQPNKDNCRRAENV a a a a c c t t c g c a g g a c a g t t c a a c c t a a t a a g g a c a a t t g c a g g c g a g c t g a a a a t g t t c t t t t g g a a g c g t c c t g t c a a g t t g g a t t a t t c c t g t t a a c g t c c g c t c g a c t t t t a c a a g

1200 LRLWIIEAKDLAPKKKYFCE t t c g t t t a t g g a t c a t t g a a g c c a a g g a c c t t g c c c c t a a a a a g a a a t a t t t c t g c g a a c a a g c a a a t a c c t a g t a a c t t c g g t t c c t g g a a c g g g g a t t t t t c t t t a t a a a g a c g c t t g

1260 LCLDDTLFARTTSKTKADNI t g t g c c t t g a t g a t a c c c t c t t t g c t c g t a c a a c c a g c a a g a c c a a a g c a g a c a a t a t t t a c a c g g a a c t a c t a t g g g a g a a a c g a g c a t g t t g g t c g t t c t g g t t t c g t c t g t t a t a a a

1320 FWGEHFEFFSLPPLHSITVH t c t g g g g c g a a c a t t t t g a a t t c t t c a g c c t t c c a c c t c t t c a t a g t a t c a c a g t t c a c a a g a c c c c g c t t g t a a a a c t t a a g a a g t c g g a a g g t g g a g a a g t a t c a t a g t g t c a a g t g t

1380 IYKDVEKKKKKDKNNYVGLV t t t a c a a g g a t g t g g a a a a a a a g a a a a a a a a g g a c a a g a a t a a t t a t g t a g g g c t a g t c a a a a t g t t c c t a c a c c t t t t t t t c t t t t t t t t c c t g t t c t t a t t a a t a c a t c c c g a t c a g t

1440 NIPTASVTGRQFVEKWYPVS a c a t c c c c a c t g c c a g t g t g a c t g g t c g c c a a t t t g t a g a a a a g t g g t a t c c a g t g a g t a t g t a g g g g t g a c g g t c a c a c t g a c c a g c g g t t a a a c a t c t t t t c a c c a t a g g t c a c t c a t

1500 TPTPNKGKTGGPSIRIKSRF c a c c t a c a c c c a a c a a a g g a a a g a c a g g a g g a c c t t c t a t t c g g a t t a a a t c a c g t t t c c g t g g a t g t g g g t t g t t t c c t t t c t g t c c t c c t g g a a g a t a a g c c t a a t t t a g t g c a a a g g

1560 QTITILPMEQYKEFAEFVTS a a a c t a t c a c c a t t c t g c c t a t g g a g c a a t a c a a a g a a t t t g c a g a a t t t g t c a c c a g c a t t t g a t a g t g g t a a g a c g g a t a c c t c g t t a t g t t t c t t a a a c g t c t t a a a c a g t g g t c g t

1620 NYTMLCSVLEPVISVRNKEE a c t a c a c c a t g c t g t g t t c t g t c c t t g a g c c a g t a a t t a g t g t g a g a a a t a a a g a g g a g t t g a t g t g g t a c g a c a c a a g a c a g g a a c t c g g t c a t t a a t c a c a c t c t t t a t t t c t c c t c a

1680 LACALVHILQSTGRAKDFLT t g g c t t g t g c c t t a g t g c a c a t t c t t c a a a g t a c t g g c a g a g c c a a g g a t t t t c t g a c t g a c c g a a c a c g g a a t c a c g t g t a a g a a g t t t c a t g a c c g t c t c g g t t c c t a a a a g a c t g a c

1740 DLVMSEVDRCGEHDVLIFRE a c t t g g t g a t g t c t g a g g t g g a t c g t t g t g g a g a g c a t g a t g t c t t g a t c t t c a g a g a g a t g a a c c a c t a c a g a c t c c a c c t a g c a a c a c c t c t c g t a c t a c a g a a c t a g a a g t c t c t c t

1800 NTIATKSIEEYLKLVGQQYL a c a c t a t t g c c a c c a a a t c c a t t g a g g a a t a c c t c a a g t t g g t g g g a c a a c a g t a t c t t c t g t g a t a a c g g t g g t t t a g g t a a c t c c t t a t g g a g t t c a a c c a c c c t g t t g t c a t a g a a g

1860 HDALGEFIKALYESDENCEV a t g a c g c a c t g g g g g a g t t t a t c a a a g c t t t g t a t g a g t c c g a t g a g a a c t g t g a a g t g g t a c t g c g t g a c c c c c t c a a a t a g t t t c g a a a c a t a c t c a g g c t a c t c t t g a c a c t t c a c c

1920 DPSKCSSSELIDHQSNLKMC a t c c c a g c a a a t g t t c a t c t a g t g a a c t g a t a g a c c a t c a g a g c a a c c t g a a a a t g t g c t t a g g g t c g t t t a c a a g t a g a t c a c t t g a c t a t c t g g t a g t c t c g t t g g a c t t t t a c a c g a

1980 CELAFCKIINSYCVFPRELK g t g a g c t g g c t t t c t g c a a g a t c a t c a a c t c t t a c t g t g t t t t c c c t c g t g a g t t g a a a g c a c t c g a c c g a a a g a c g t t c t a g t a g t t g a g a a t g a c a c a a a a g g g a g c a c t c a a c t t t c

2040 EVFASWKQQCLNRGKQDISE a a g t g t t t g c a t c a t g g a a g c a g c a g t g c c t g a a c c g t g g c a a g c a a g a c a t c a g c g a g a t t c a c a a a c g t a g t a c c t t c g t c g t c a c g g a c t t g g c a c c g t t c g t t c t g t a g t c g c t c t

2100 RLISASLFLRFLCPAIMSPS g g c t c a t c a g t g c c t c a t t a t t t c t c c g t t t t c t g t g t c c a g c c a t t a t g t c t c c c a g t c c c g a g t a g t c a c g g a g t a a t a a a g a g g c a a a a g a c a c a g g t c g g t a a t a c a g a g g g t c a g

2160 LFNLMQEYPDDRTSRTLTLI t t t t c a a c c t t a t g c a g g a g t a t c c t g a t g a c c g c a c a t c t c g g a c t c t a a c t c t t a t t g a a a a g t t g g a a t a c g t c c t c a t a g g a c t a c t g g c g t g t a g a g c c t g a g a t t g a g a a t a a c

2220 AKVIQNLANFAKFGNKEEYM c c a a g g t c a t t c a g a a c c t g g c c a a c t t t g c c a a g t t t g g t a a c a a a g a g g a a t a c a t g g g g t t c c a g t a a g t c t t g g a c c g g t t g a a a c g g t t c a a a c c a t t g t t t c t c c t t a t g t a c c

BglIIAfeI 2280 AFMNDFLEHEWGGMKRFLLE c a t t c a t g a a t g a t t t t t t a g a a c a t g a a t g g g g t g g a a t g a a g c g c t t t c t t t t g g a g a g t a a g t a c t t a c t a a a a a a t c t t g t a c t t a c c c c a c c t t a c t t c g c g a a a g a a a a c c t c t

2340 ISNPDTISNTPGFDGYIDLG t c t c t a a t c c a g a c a c c a t c t c a a a c a c c c c a g g c t t t g a t g g t t a c a t t g a t c t g g g c c a g a g a t t a g g t c t g t g g t a g a g t t t g t g g g g t c c g a a a c t a c c a a t g t a a c t a g a c c c g g

NmeAIII 2400 RELSVLHSLLWEVVSQLDKA g a g a g c t t t c a g t t t t g c a t t c c t t a c t g t g g g a a g t a g t t t c c c a a c t t g a t a a g g c g a c t c t c g a a a g t c a a a a c g t a a g g a a t g a c a c c c t t c a t c a a a g g g t t g a a c t a t t c c g c t

2460 TVAKLGPLPRVLADITKSLT c c g t g g c a a a a t t g g g g c c t c t c c c t c g t g t t c t t g c t g a t a t t a c c a a g t c a t t g a c t a g g c a c c g t t t t a a c c c c g g a g a g g g a g c a c a a g a a c g a c t a t a a t g g t t c a g t a a c t g a t

2520 NPTPIQQQLRRFTEHNSSPN a t c c t a c g c c a a t a c a a c a g c a a c t g a g a c g c t t c a c t g a a c a t a a c t c c a g t c c a a a t g t a g g a t g c g g t t a t g t t g t c g t t g a c t c t g c g a a g t g a c t t g t a t t g a g g t c a g g t t t a c

PstI 2580 VSGSLSSGLQKIFEDPTDSD t c a g t g g a a g c c t c t c c t c t g g g c t g c a g a a a a t a t t t g a a g a c c c c a c t g a c a g t g a t t a g t c a c c t t c g g a g a g g a g a c c c g a c g t c t t t t a t a a a c t t c t g g g g t g a c t g t c a c t a a

2640 LHKLKSPSQDNTDSYFRGKT t g c a t a a a c t a a a a t c t c c a a g c c a g g a c a a c a c a g a c a g c t a c t t c a g a g g g a a a a c a t a c g t a t t t g a t t t t a g a g g t t c g g t c c t g t t g t g t c t g t c g a t g a a g t c t c c c t t t t g t a

2700 LLLVQQASSQSMTYSEKDER t a t t g c t g g t t c a g c a a g c c t c c t c t c a g a g c a t g a c t t a t t c t g a a a a g g a t g a a a g g g a t a a c g a c c a a g t c g t t c g g a g g a g a g t c t c g t a c t g a a t a a g a c t t t t c c t a c t t t c c c

PfoI* 2760 ESSLPNGRSVSLMDLQDTHA a a a g t a g c c t t c c t a a t g g t c g g a g c g t c t c c c t c a t g g a c c t c c a g g a c a c t c a t g c t g t t t c a t c g g a a g g a t t a c c a g c c t c g c a g a g g g a g t a c c t g g a g g t c c t g t g a g t a c g a c

NsiI 2820 AQVEHASVMLDVPIRLTGSQ c t c a a g t g g a g c a t g c a t c t g t c a t g c t t g a t g t g c c t a t a c g c t t g a c c g g a a g c c a g c g a g t t c a c c t c g t a c g t a g a c a g t a c g a a c t a c a c g g a t a t g c g a a c t g g c c t t c g g t c g

2880 LSITQVASIKQLRETQSTPQ t t t c c a t a a c c c a g g t g g c c a g c a t c a a a c a g c t g c g g g a a a c c c a g a g c a c t c c c c a a a a a a g g t a t t g g g t c c a c c g g t c g t a g t t t g t c g a c g c c c t t t g g g t c t c g t g a g g g g t t t

2940 SAPQVRRPLHPALNQPGGLQ g t g c a c c c c a a g t g a g a a g g c c c c t g c a c c c a g c c t t g a a c c a g c c a g g t g g c c t t c a g c c a c g t g g g g t t c a c t c t t c c g g g g a c g t g g g t c g g a a c t t g g t c g g t c c a c c g g a a g t c g

StuI 3000 PLSFQNPVYHLNNPIPAMPK c c t t g t c g t t c c a g a a c c c t g t c t a t c a c c t c a a t a a c c c a a t t c c a g c a a t g c c a a a g g g g a a c a g c a a g g t c t t g g g a c a g a t a g t g g a g t t a t t g g g t t a a g g t c g t t a c g g t t t c c

Bpu10I 3060 ASIDSSLENLSTASSRSQSN c c t c t a t a g a t t c c a g t t t g g a g a a c c t a a g c a c t g c c a g t t c c a g a a g c c a a a g t a a c a g g a g a t a t c t a a g g t c a a a c c t c t t g g a t t c g t g a c g g t c a a g g t c t t c g g t t t c a t t g t

3120 SEDFKLSGPSNSSMEDFTKR g t g a a g a c t t c a a g c t c a g t g g a c c c a g c a a t a g c a g c a t g g a a g a t t t c a c t a a a c g t a c a c t t c t g a a g t t c g a g t c a c c t g g g t c g t t a t c g t c g t a c c t t c t a a a g t g a t t t g c a t

3180 STQSEDFSRRHTVPDRHIPL g c a c t c a g a g t g a g g a c t t c t c c a g g c g g c a c a c g g t g c c a g a t a g a c a c a t a c c t c t t g c g t g a g t c t c a c t c c t g a a g a g g t c c g c c g t g t g c c a c g g t c t a t c t g t g t a t g g a g a a c

3240 ALPRQNSTGQAQIRKVDQGG c t t t g c c a c g a c a a a a t a g t a c t g g g c a g g c c c a g a t c c g a a a a g t g g a c c a g g g t g g g t g a a a c g g t g c t g t t t t a t c a t g a c c c g t c c g g g t c t a g g c t t t t c a c c t g g t c c c a c c c a

SnaBI 3300 LGARAKAPPSLPHSASLRST t a g g t g c c c g a g c c a a a g c c c c a c c a t c c c t g c c a c a c a g t g c t t c t t t a c g t a g c a c c g a t c c a c g g g c t c g g t t t c g g g g t g g t a g g g a c g g t g t g t c a c g a a g a a a t g c a t c g t g g c

3360 GSMSVVSAALVAEPVQNGSR g g a g c a t g t c a g t g g t g t c c g c a g c c c t g g t g g c c g a a c c t g t g c a g a a t g g g a g c c g g t c c t c g t a c a g t c a c c a c a g g c g t c g g g a c c a c c g g c t t g g a c a c g t c t t a c c c t c g g c c a

3420 SRQQSSSSRESPVPKVRAIQ c c c g g c a g c a g t c c t c t t c c t c c a g a g a g a g c c c t g t t c c c a a a g t t a g a g c a a t c c a g a g g g c c g t c g t c a g g a g a a g g a g g t c t c t c t c g g g a c a a g g g t t t c a a t c t c g t t a g g t c t

3480 RQQTQQVQSPVDSATMSPVE g a c a a c a g a c a c a g c a g g t t c a g t c a c c t g t g g a c t c t g c c a c a a t g t c c c c a g t a g a g a c t g t t g t c t g t g t c g t c c a a g t c a g t g g a c a c c t g a g a c g g t g t t a c a g g g g t c a t c t c t

3540 RTAAWVLNNGQYEEDVEETE g g a c a g c a g c c t g g g t t c t g a a c a a t g g g c a g t a t g a a g a g g a t g t g g a a g a a a c t g a g c c c t g t c g t c g g a c c c a a g a c t t g t t a c c c g t c a t a c t t c t c c t a c a c c t t c t t t g a c t c g

3600 QNLDEAKHAEKYEQEITKLK a a a a t c t a g a t g a a g c c a a g c a t g c t g a g a a g t a t g a a c a a g a a a t t a c t a a a c t g a a g g t t t t a g a t c t a c t t c g g t t c g t a c g a c t c t t c a t a c t t g t t c t t t a a t g a t t t g a c t t c c

3660 ERLRVSSRRLEEYERRLLVQ a g c g c c t g a g a g t t t c c a g c c g g c g a c t g g a g g a a t a t g a a c g c c g c t t g c t g g t g c a g g t c g c g g a c t c t c a a a g g t c g g c c g c t g a c c t c c t t a t a c t t g c g g c g a a c g a c c a c g t c c

3720 EQQMQKLLLEYKARLEDSEE a g c a g c a g a t g c a g a a g c t g c t g c t g g a a t a c a a g g c c c g a c t g g a g g a c a g c g a g g a g c t c g t c g t c t a c g t c t t c g a c g a c g a c c t t a t g t t c c g g g c t g a c c t c c t g t c g c t c c t c g

3780 RLRRQQEEKDSQMKSIISRL g g c t c c g a a g a c a g c a g g a a g a a a a a g a t a g c c a g a t g a a a a g c a t c a t c a g c a g g c t a a c c g a g g c t t c t g t c g t c c t t c t t t t t c t a t c g g t c t a c t t t t c g t a g t a g t c g t c c g a t t

3840 MAVEEELKKDHAEMQAVIDA t g g c t g t g g a a g a g g a a c t g a a g a a g g a t c a t g c t g a g a t g c a a g c a g t t a t t g a t g c a a a c c g a c a c c t t c t c c t t g a c t t c t t c c t a g t a c g a c t c t a c g t t c g t c a a t a a c t a c g t t

3900 KQKIIDAQEKRIVSLDSANT a g c a g a a a a t a a t t g a t g c a c a g g a a a a a c g g a t c g t g t c c c t g g a t t c a g c c a a c a c c a t c g t c t t t t a t t a a c t a c g t g t c c t t t t t g c c t a g c a c a g g g a c c t a a g t c g g t t g t g g t

3960 RLMSALTQVKERYSMQVRNG g a c t g a t g a g c g c g c t g a c c c a a g t g a a g g a g c g g t a c a g c a t g c a g g t c c g c a a t g g c a c t g a c t a c t c g c g c g a c t g g g t t c a c t t c c t c g c c a t g t c g t a c g t c c a g g c g t t a c c g t

4020 ISPTNPTKLSITENGEFKNS t c t c c c c c a c c a a c c c c a c c a a g c t t t c c a t c a c g g a g a a t g g t g a a t t c a a a a a c a g c a a g a g g g g g t g g t t g g g g t g g t t c g a a a g g t a g t g c c t c t t a c c a c t t a a g t t t t t g t c g t

4080 attL2 SCSTFLYKVGIIGKHCLSIC g c t g c t c a a c t t t c t t g t a c a a a g t t g g c a t t a t a g g a a a g c a t t g c t t a t c a a t t t g t t c g a c g a g t t g a a a g a a c a t g t t t c a a c c g t a a t a t c c t t t c g t a a c g a a t a g t t a a a c a a

4140 attL2 CNEQVTISQNKIIICMQA* g c a a c g a a c a g g t c a c t a t c a g t c a a a a t a a a a t c a t t a t t t g c a t g c a a g c t t a g a t c c c g t t g c t t g t c c a g t g a t a g t c a g t t t t a t t t t a g t a a t a a a c g t a c g t t c g a a t c t a g g

4200 t c t a g a c c a g c c a g g a c a g a a a t g c c t c g a c t t c g c t g c t a c c c a a g g t t g c c g g g t g a c a g a t c t g g t c g g t c c t g t c t t t a c g g a g c t g a a g c g a c g a t g g g t t c c a a c g g c c c a c t g

4260 g c a c a c c g t g g a a a c g g a t g a a g g c a c g a a c c c a g t g g a c a t a a g c c t g t t c g g t t c g t a c g t g t g g c a c c t t t g c c t a c t t c c g t g c t t g g g t c a c c t g t a t t c g g a c a a g c c a a g c a t

4320 MRSRNWSRTLTER a g c t g t a a t g c a a g t a g c g t a t g c g c t c a c g c a a c t g g t c c a g a a c c t t g a c c g a a c g c a t c g a c a t t a c g t t c a t c g c a t a c g c g a g t g c g t t g a c c a g g t c t t g g a a c t g g c t t g c g t

4380 SGGNGAVAVFMACYDCFFGV g c g g t g g t a a c g g c g c a g t g g c g g t t t t c a t g g c t t g t t a t g a c t g t t t t t t t g g g g t a c c g c c a c c a t t g c c g c g t c a c c g c c a a a a g t a c c g a a c a a t a c t g a c a a a a a a a c c c c a t g

4440 QSMPRASKQQARYAVGRCLM a g t c t a t g c c t c g g g c a t c c a a g c a g c a a g c g c g t t a c g c c g t g g g t c g a t g t t t g a t g t t c a g a t a c g g a g c c c g t a g g t t c g t c g t t c g c g c a a t g c g g c a c c c a g c t a c a a a c t a c a

4500 SmR LWSSNDVTQQGSRPKTKLNI t a t g g a g c a g c a a c g a t g t t a c g c a g c a g g g c a g t c g c c c t a a a a c a a a g t t a a a c a t t a a t a c c t c g t c g t t g c t a c a a t g c g t c g t c c c g t c a g c g g g a t t t t g t t t c a a t t t g t a a t

4560 1 5 10 15 20 MREAVIAEVSTQLSEVVGVI SmR MREAVIAEVSTQLSEVVGVI t g a g g g a a g c g g t g a t c g c c g a a g t a t c g a c t c a a c t a t c a g a g g t a g t t g g c g t c a t c g a c t c c c t t c g c c a c t a g c g g c t t c a t a g c t g a g t t g a t a g t c t c c a t c a a c c g c a g t a g c

4620 25 30 35 40 ERHLEPTLLAVHLYGSAVDG SmR ERHLEPTLLAVHLYGSAVDG a g c g c c a t c t c g a a c c g a c g t t g c t g g c c g t a c a t t t g t a c g g c t c c g c a g t g g a t g g c g t c g c g g t a g a g c t t g g c t g c a a c g a c c g g c a t g t a a a c a t g c c g a g g c g t c a c c t a c c g c

4680 45 50 55 60 GLKPHSDIDLLVTVTVRLDE SmR GLKPHSDIDLLVTVTVRLDE g c c t g a a g c c a c a c a g t g a t a t t g a t t t g c t g g t t a c g g t g a c c g t a a g g c t t g a t g a a a c g g a c t t c g g t g t g t c a c t a t a a c t a a a c g a c c a a t g c c a c t g g c a t t c c g a a c t a c t t t

BclI* 4740 65 70 75 80 TTRRALINDLLETSASPGES SmR TTRRALINDLLETSASPGES c a a c g c g g c g a g c t t t g a t c a a c g a c c t t t t g g a a a c t t c g g c t t c c c c t g g a g a g a g c g g t t g c g c c g c t c g a a a c t a g t t g c t g g a a a a c c t t t g a a g c c g a a g g g g a c c t c t c t c g c

4800 85 90 95 100 EILRAVEVTIVVHDDIIPWR SmR EILRAVEVTIVVHDDIIPWR a g a t t c t c c g c g c t g t a g a a g t c a c c a t t g t t g t g c a c g a c g a c a t c a t t c c g t g g c g t t t c t a a g a g g c g c g a c a t c t t c a g t g g t a a c a a c a c g t g c t g c t g t a g t a a g g c a c c g c a a

4860 105 110 115 120 YPAKRELQFGEWQRNDILAG SmR YPAKRELQFGEWQRNDILAG a t c c a g c t a a g c g c g a a c t g c a a t t t g g a g a a t g g c a g c g c a a t g a c a t t c t t g c a g g t a t a g g t c g a t t c g c g c t t g a c g t t a a a c c t c t t a c c g t c g c g t t a c t g t a a g a a c g t c c a t

PvuI 4920 125 130 135 140 IFEPATIDIDLAILLTKARE SmR IFEPATIDIDLAILLTKARE t c t t c g a g c c a g c c a c g a t c g a c a t t g a t c t g g c t a t c t t g c t g a c a a a a g c a a g a g a a c a g a a g c t c g g t c g g t g c t a g c t g t a a c t a g a c c g a t a g a a c g a c t g t t t t c g t t c t c t t g

4980 145 150 155 160 HSVALVGPAAEELFDPVPEQ SmR HSVALVGPAAEELFDPVPEQ a t a g c g t t g c c t t g g t a g g t c c a g c g g c g g a g g a a c t c t t t g a t c c g g t t c c t g a a c a g g t a t c g c a a c g g a a c c a t c c a g g t c g c c g c c t c c t t g a g a a a c t a g g c c a a g g a c t t g t c c

5040 165 170 175 180 DLFEALNETLTLWNSPPDWA SmR DLFEALNETLTLWNSPPDWA a t c t a t t t g a g g c g c t a a a t g a a a c c t t a a c g c t a t g g a a c t c g c c g c c c g a c t g g g c t g t a g a t a a a c t c c g c g a t t t a c t t t g g a a t t g c g a t a c c t t g a g c g g c g g g c t g a c c c g a c

5100 185 190 195 200 GDERNVVLTLSRIWYSAVTG SmR GDERNVVLTLSRIWYSAVTG g c g a t g a g c g a a a t g t a g t g c t t a c g t t g t c c c g c a t t t g g t a c a g c g c a g t a a c c g g c a c g c t a c t c g c t t t a c a t c a c g a a t g c a a c a g g g c g t a a a c c a t g t c g c g t c a t t g g c c g t

5160 205 210 215 220 KIAPKDVAADWAMERLPAQY SmR KIAPKDVAADWAMERLPAQY a a a t c g c g c c g a a g g a t g t c g c t g c c g a c t g g g c a a t g g a g c g c c t g c c g g c c c a g t a t c t t t a g c g c g g c t t c c t a c a g c g a c g g c t g a c c c g t t a c c t c g c g g a c g g c c g g g t c a t a g

5220 225 230 235 240 QPVILEARQAYLGQEEDRLA SmR QPVILEARQAYLGQEEDRLA a g c c c g t c a t a c t t g a a g c t a g a c a g g c t t a t c t t g g a c a a g a a g a a g a t c g c t t g g c c t t c g g g c a g t a t g a a c t t c g a t c t g t c c g a a t a g a a c c t g t t c t t c t t c t a g c g a a c c g g a

5280 245 250 255 260 SRADQLEEFVHYVKGEITKV SmR SRADQLEEFVHYVKGEITKV c g c g c g c a g a t c a g t t g g a a g a a t t t g t c c a c t a c g t g a a a g g c g a g a t c a c c a a g g t a g g c g c g c g t c t a g t c a a c c t t c t t a a a c a g g t g a t g c a c t t t c c g c t c t a g t g g t t c c a t c

PaeR7IPspXIXhoI 5340 VGK* SmR VGK* t c g g c a a a t a a c c c t c g a g c c a c c c a t g a c c a a a a t c c c t t a a c g t g a g t t a c g c g t c g t a g c c g t t t a t t g g g a g c t c g g t g g g t a c t g g t t t t a g g g a a t t g c a c t c a a t g c g c a g c a

5400 ori t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g

5460 ori t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g

5520 ori c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g

5580 ori c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g

5640 ori c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a

5700 ori c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a

5760 ori g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a

5820 ori a c c t a c a g c g t g a g c a t t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t t g g a t g t c g c a c t c g t a a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a

5880 ori G G G A A A C G pBR322ori-F a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c

5940 ori C C T G G T A T C T T T pBR322ori-F c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a

6000 ori g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a

PciI 6060 t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c

6120 t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c

BspQISapI 6180 A G C G A G T C A G T G A G C G A G L4440 a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g

6240 c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c

6300 g c a g t g a g c g c a a c g c a a t t a a t a c g c g t a c c g c t a g c c a g g a a g a g t t t g t a g a a a c g c c g t c a c t c g c g t t g c g t t a a t t a t g c g c a t g g c g a t c g g t c c t t c t c a a a c a t c t t t g c g

6360 rrnB T2 terminator a a a a a g g c c a t c c g t c a g g a t g g c c t t c t g c t t a g t t t g a t g c c t g g c a g t t t a t g g c g g t t t t t c c g g t a g g c a g t c c t a c c g g a a g a c g a a t c a a a c t a c g g a c c g t c a a a t a c c g c c

AclI 6420 g c g t c c t g c c c g c c a c c c t c c g g g c c g t t g c t t c a c a a c g t t c a a a t c c g c t c c c g g c g g c g c a g g a c g g g c g g t g g g a g g c c c g g c a a c g a a g t g t t g c a a g t t t a g g c g a g g g c c g c c

6480 rrnB T1 terminator a t t t g t c c t a c t c a g g a g a g c g t t c a c c g a c a a a c a a c a g a t a a a a c g a a a g g c c c a g t c t a a a c a g g a t g a g t c c t c t c g c a a g t g g c t g t t t g t t g t c t a t t t t g c t t t c c g g g t c a g

3ʹ 5ʹ 6533 rrnB T1 terminator t t c c g a c t g a g c c t t t c g t t t t a t t t g a t g c c t g g c a g t t c c c t a c t c t c g c g a a g g c t g a c t c g g a a a g c a a a a t a a a c t a c g g a c c g t c a a g g g a t g a g a g c g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.