Addgene R777-E155 Hs.PDGFRA - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: R777-E155 Hs.PDGFRA


Map View

M13/pUC Forward (23 .. 45) rrnB T2 terminator L4440 (5556 .. 5573) PciI (5458) pBR322ori-F (5303 .. 5322) BssSI - BssSαI (5285) BciVI (5260) PaeR7I - PspXI - XhoI (4724) BsaAI (4685) BssHII (4652) NaeI (4579) NgoMIV (4577) PvuI (4309) HindIII (3559) CsiI - SexAI * (3430) BsmBI - Esp3I (3330) Bsu36I (3271) AfeI (3224) M13 Forward (37 .. 54) AhdI (50) RsrII (71) BamHI (78) PsiI (153) EcoRI (308) NmeAIII (436) PspOMI (738) ApaI (742) DraI (808) PfoI * (1566) PstI (1775) BsaBI * (1797) PflMI (1956) SpeI (1961) BspHI (2047) SspI (2445) StuI (2551) BstBI (2724) BsmI (2830) AccI (2959) R777-E155 Hs.PDGFRA 5963 bp

Sequence View

AhdI 60 M13 fwd C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward t t a a c g c t a g c a t g g a t g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t c t t a a a a a t t g c g a t c g t a c c t a c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a g a a t t t

BamHIRsrII 120 attL1 a t t c g a g c t c g g t c c g a g g a t c c g a a a t a a t g a t t t t a t t t t g a c t g a t a g t g a c c t g t t t a a g c t c g a g c c a g g c t c c t a g g c t t t a t t a c t a a a a t a a a a c t g a c t a t c a c t g g a c a a

PsiI 180 attL1 MPTLYKKV c g t t g c a a c a a a t t g a t a a g c a a t g c t t t t t t a t a a t g c c a a c t t t g t a c a a a a a a g t t g g c a a c g t t g t t t a a c t a t t c g t t a c g a a a a a a t a t t a c g g t t g a a a c a t g t t t t t t c a a c

240 attL1 GTMGTSHPAFLVLGCLLTGL g c a c c a t g g g g a c t t c c c a t c c g g c g t t c c t g g t c t t a g g c t g t c t t c t c a c a g g g c t g a c g t g g t a c c c c t g a a g g g t a g g c c g c a a g g a c c a g a a t c c g a c a g a a g a g t g t c c c g a c t

300 SLILCQLSLPSILPNENEKV g c c t a a t c c t c t g c c a g c t t t c a t t a c c c t c t a t c c t t c c a a a t g a a a a t g a a a a g g t t g c g g a t t a g g a g a c g g t c g a a a g t a a t g g g a g a t a g g a a g g t t t a c t t t t a c t t t t c c a a c

EcoRI 360 VQLNSSFSLRCFGESEVSWQ t g c a g c t g a a t t c a t c c t t t t c t c t g a g a t g c t t t g g g g a g a g t g a a g t g a g c t g g c a g t a c g t c g a c t t a a g t a g g a a a a g a g a c t c t a c g a a a c c c c t c t c a c t t c a c t c g a c c g t c a

420 YPMSEEESSDVEIRNEENNS a c c c c a t g t c t g a a g a a g a g a g c t c c g a t g t g g a a a t c a g a a a t g a a g a a a a c a a c a g c g t g g g g t a c a g a c t t c t t c t c t c g a g g c t a c a c c t t t a g t c t t t a c t t c t t t t g t t g t c g c

NmeAIII 480 GLFVTVLEVSSASAAHTGLY g c c t t t t t g t g a c g g t c t t g g a a g t g a g c a g t g c c t c g g c g g c c c a c a c a g g g t t g t a c a c g g a a a a a c a c t g c c a g a a c c t t c a c t c g t c a c g g a g c c g c c g g g t g t g t c c c a a c a t g t

540 TCYYNHTQTEENELEGRHIY c t t g c t a t t a c a a c c a c a c t c a g a c a g a a g a g a a t g a g c t t g a a g g c a g g c a c a t t t a c a g a a c g a t a a t g t t g g t g t g a g t c t g t c t t c t c t t a c t c g a a c t t c c g t c c g t g t a a a t g t

600 IYVPDPDVAFVPLGMTDYLV t c t a t g t g c c a g a c c c a g a t g t a g c c t t t g t a c c t c t a g g a a t g a c g g a t t a t t t a g t c a a g a t a c a c g g t c t g g g t c t a c a t c g g a a a c a t g g a g a t c c t t a c t g c c t a a t a a a t c a g t

660 IVEDDDSAIIPCRTTDPETP t c g t g g a g g a t g a t g a t t c t g c c a t t a t a c c t t g t c g c a c a a c t g a t c c c g a g a c t c c t g a g c a c c t c c t a c t a c t a a g a c g g t a a t a t g g a a c a g c g t g t t g a c t a g g g c t c t g a g g a c

720 VTLHNSEGVVPASYDSRQGF t a a c c t t a c a c a a c a g t g a g g g g g t g g t a c c t g c c t c c t a c g a c a g c a g a c a g g g c t t t a a t t g g a a t g t g t t g t c a c t c c c c c a c c a t g g a c g g a g g a t g c t g t c g t c t g t c c c g a a a t

PspOMIApaI 780 NGTFTVGPYICEATVKGKKF a t g g g a c c t t c a c t g t a g g g c c c t a t a t c t g t g a g g c c a c c g t c a a a g g a a a g a a g t t c c t a c c c t g g a a g t g a c a t c c c g g g a t a t a g a c a c t c c g g t g g c a g t t t c c t t t c t t c a a g g

DraI 840 QTIPFNVYALKATSELDLEM a g a c c a t c c c a t t t a a c g t t t a t g c t t t a a a a g c a a c a t c a g a g c t g g a t c t a g a a a t g g t c t g g t a g g g t a a a t t g c a a a t a c g a a a t t t t c g t t g t a g t c t c g a c c t a g a t c t t t a c c

900 EALKTVYKSGETIVVTCAVF a a g c t c t t a a a a c c g t g t a t a a g t c a g g g g a a a c g a t t g t g g t c a c c t g t g c t g t t t t t a t t c g a g a a t t t t g g c a c a t a t t c a g t c c c c t t t g c t a a c a c c a g t g g a c a c g a c a a a a a t

960 NNEVVDLQWTYPGEVKGKGI a c a a t g a g g t g g t t g a c c t t c a a t g g a c t t a c c c t g g a g a a g t g a a a g g c a a a g g c a t c a t g t t a c t c c a c c a a c t g g a a g t t a c c t g a a t g g g a c c t c t t c a c t t t c c g t t t c c g t a g t

1020 TMLEEIKVPSIKLVYTLTVP c a a t g c t g g a a g a a a t c a a a g t c c c a t c c a t c a a a t t g g t g t a c a c t t t g a c g g t c c c c g g t t a c g a c c t t c t t t a g t t t c a g g g t a g g t a g t t t a a c c a c a t g t g a a a c t g c c a g g g g c

1080 EATVKDSGDYECAARQATRE a g g c c a c g g t g a a a g a c a g t g g a g a t t a c g a a t g t g c t g c c c g c c a g g c t a c c a g g g a g g t c c g g t g c c a c t t t c t g t c a c c t c t a a t g c t t a c a c g a c g g g c g g t c c g a t g g t c c c t c c

1140 VKEMKKVTISVHEKGFIEIK t c a a a g a a a t g a a g a a a g t c a c t a t t t c t g t c c a t g a g a a a g g g t t c a t t g a a a t c a a a c a g t t t c t t t a c t t c t t t c a g t g a t a a a g a c a g g t a c t c t t t c c c a a g t a a c t t t a g t t t g

1200 PTFSQLEAVNLHEVKHFVVE c c a c c t t c a g c c a g t t g g a a g c t g t c a a c c t g c a t g a a g t c a a a c a t t t t g t t g t a g a g g g g t g g a a g t c g g t c a a c c t t c g a c a g t t g g a c g t a c t t c a g t t t g t a a a a c a a c a t c t c c

1260 VRAYPPPRISWLKNNLTLIE t g c g g g c c t a c c c a c c t c c c a g g a t a t c c t g g c t g a a a a a c a a t c t g a c t c t g a t t g a a a a c g c c c g g a t g g g t g g a g g g t c c t a t a g g a c c g a c t t t t t g t t a g a c t g a g a c t a a c t t t

1320 NLTEITTDVEKIQEIRYRSK a t c t c a c t g a g a t c a c c a c t g a t g t g g a a a a g a t t c a g g a a a t a a g g t a t c g a a g c a a a t t a g a g t g a c t c t a g t g g t g a c t a c a c c t t t t c t a a g t c c t t t a t t c c a t a g c t t c g t t t a

1380 LKLIRAKEEDSGHYTIVAQN t a a a g c t g a t c c g t g c t a a g g a a g a a g a c a g t g g c c a t t a t a c t a t t g t a g c t c a a a a t g a t t t c g a c t a g g c a c g a t t c c t t c t t c t g t c a c c g g t a a t a t g a t a a c a t c g a g t t t t a c

1440 EDAVKSYTFELLTQVPSSIL a a g a t g c t g t g a a g a g c t a t a c t t t t g a a c t g t t a a c t c a a g t t c c t t c a t c c a t t c t g g t t c t a c g a c a c t t c t c g a t a t g a a a a c t t g a c a a t t g a g t t c a a g g a a g t a g g t a a g a c c

1500 DLVDDHHGSTGGQTVRCTAE a c t t g g t c g a t g a t c a c c a t g g c t c a a c t g g g g g a c a g a c g g t g a g g t g c a c a g c t g a a g t g a a c c a g c t a c t a g t g g t a c c g a g t t g a c c c c c t g t c t g c c a c t c c a c g t g t c g a c t t c

1560 GTPLPDIEWMICKDIKKCNN g c a c g c c g c t t c c t g a t a t t g a g t g g a t g a t a t g c a a a g a t a t t a a g a a a t g t a a t a a t g c g t g c g g c g a a g g a c t a t a a c t c a c c t a c t a t a c g t t t c t a t a a t t c t t t a c a t t a t t a c

PfoI* 1620 ETSWTILANNVSNIITEIHS a a a c t t c c t g g a c t a t t t t g g c c a a c a a t g t c t c a a a c a t c a t c a c g g a g a t c c a c t c c c t t t g a a g g a c c t g a t a a a a c c g g t t g t t a c a g a g t t t g t a g t a g t g c c t c t a g g t g a g g g

1680 RDRSTVEGRVTFAKVEETIA g a g a c a g g a g t a c c g t g g a g g g c c g t g t g a c t t t c g c c a a a g t g g a g g a g a c c a t c g c c g c t c t g t c c t c a t g g c a c c t c c c g g c a c a c t g a a a g c g g t t t c a c c t c c t c t g g t a g c g g c

1740 VRCLAKNLLGAENRELKLVA t g c g a t g c c t g g c t a a g a a t c t c c t t g g a g c t g a g a a c c g a g a g c t g a a g c t g g t g g c t c a c g c t a c g g a c c g a t t c t t a g a g g a a c c t c g a c t c t t g g c t c t c g a c t t c g a c c a c c g a g

BsaBI*PstI 1800 PTLRSELTVAAAVLVLLVIV c c a c c c t g c g t t c t g a a c t c a c g g t g g c t g c t g c a g t c c t g g t g c t g t t g g t g a t t g t g a g g t g g g a c g c a a g a c t t g a g t g c c a c c g a c g a c g t c a g g a c c a c g a c a a c c a c t a a c a c t

1860 IISLIVLVVIWKQKPRYEIR t c a t c t c a c t t a t t g t c c t g g t t g t c a t t t g g a a a c a g a a a c c g a g g t a t g a a a t t c g c t a g t a g a g t g a a t a a c a g g a c c a a c a g t a a a c c t t t g t c t t t g g c t c c a t a c t t t a a g c g a

1920 WRVIESISPDGHEYIYVDPM g g a g g g t c a t t g a a t c a a t c a g c c c g g a t g g a c a t g a a t a t a t t t a t g t g g a c c c g a t g c c c t c c c a g t a a c t t a g t t a g t c g g g c c t a c c t g t a c t t a t a t a a a t a c a c c t g g g c t a c g

SpeIPflMI 1980 QLPYDSRWEFPRDGLVLGRV a g c t g c c t t a t g a c t c a a g a t g g g a g t t t c c a a g a g a t g g a c t a g t g c t t g g t c g g g t c t t c g a c g g a a t a c t g a g t t c t a c c c t c a a a g g t t c t c t a c c t g a t c a c g a a c c a g c c c a g a

2040 LGSGAFGKVVEGTAYGLSRS t g g g g t c t g g a g c a t t t g g g a a g g t g g t t g a a g g a a c a g c c t a t g g a t t a a g c c g g t c c c a c c c c a g a c c t c g t a a a c c c t t c c a c c a a c t t c c t t g t c g g a t a c c t a a t t c g g c c a g g g

BspHI 2100 QPVMKVAVKMLKPTARSSEK a a c c t g t c a t g a a a g t t g c a g t g a a g a t g c t a a a a c c c a c g g c c a g a t c c a g t g a a a a a c t t g g a c a g t a c t t t c a a c g t c a c t t c t a c g a t t t t g g g t g c c g g t c t a g g t c a c t t t t t g

2160 QALMSELKIMTHLGPHLNIV a a g c t c t c a t g t c t g a a c t g a a g a t a a t g a c t c a c c t g g g g c c a c a t t t g a a c a t t g t a a t t c g a g a g t a c a g a c t t g a c t t c t a t t a c t g a g t g g a c c c c g g t g t a a a c t t g t a a c a t t

2220 NLLGACTKSGPIYIITEYCF a c t t g c t g g g a g c c t g c a c c a a g t c a g g c c c c a t t t a c a t c a t c a c a g a g t a t t g c t t c t t g a a c g a c c c t c g g a c g t g g t t c a g t c c g g g g t a a a t g t a g t a g t g t c t c a t a a c g a a g a

2280 YGDLVNYLHKNRDSFLSHHP a t g g a g a t t t g g t c a a c t a t t t g c a t a a g a a t a g g g a t a g c t t c c t g a g c c a c c a c c c a g t a c c t c t a a a c c a g t t g a t a a a c g t a t t c t t a t c c c t a t c g a a g g a c t c g g t g g t g g g t c

2340 EKPKKELDIFGLNPADESTR a g a a g c c a a a g a a a g a g c t g g a t a t c t t t g g a t t g a a c c c t g c t g a t g a a a g c a c a c g g a t c t t c g g t t t c t t t c t c g a c c t a t a g a a a c c t a a c t t g g g a c g a c t a c t t t c g t g t g c c t

2400 SYVILSFENNGDYMDMKQAD g c t a t g t t a t t t t a t c t t t t g a a a a c a a t g g t g a c t a c a t g g a c a t g a a g c a g g c t g a t a c g a t a c a a t a a a a t a g a a a a c t t t t g t t a c c a c t g a t g t a c c t g t a c t t c g t c c g a c t a t

SspI 2460 TTQYVPMLERKEVSKYSDIQ c t a c a c a g t a t g t c c c c a t g c t a g a a a g g a a a g a g g t t t c t a a a t a t t c c g a c a t c c a g a g a t g t g t c a t a c a g g g g t a c g a t c t t t c c t t t c t c c a a a g a t t t a t a a g g c t g t a g g t c t

2520 RSLYDRPASYKKKSMLDSEV g a t c a c t c t a t g a t c g t c c a g c c t c a t a t a a g a a g a a a t c t a t g t t a g a c t c a g a a g t c a c t a g t g a g a t a c t a g c a g g t c g g a g t a t a t t c t t c t t t a g a t a c a a t c t g a g t c t t c a g t

StuI 2580 KNLLSDDNSEGLTLLDLLSF a a a a c c t c c t t t c a g a t g a t a a c t c a g a a g g c c t t a c t t t a t t g g a t t t g t t g a g c t t c a t t t t g g a g g a a a g t c t a c t a t t g a g t c t t c c g g a a t g a a a t a a c c t a a a c a a c t c g a a g t

2640 TYQVARGMEFLASKNCVHRD *FITDVTI c c t a t c a a g t t g c c c g a g g a a t g g a g t t t t t g g c t t c a a a a a a t t g t g t c c a c c g t g a t c g g a t a g t t c a a c g g g c t c c t t a c c t c a a a a a c c g a a g t t t t t t a a c a c a g g t g g c a c t a g

2700 LAARNVLLAQGKIVKICDFG QSSAVDEQCLSFNHLDTVKA t g g c t g c t c g c a a c g t c c t c c t g g c a c a a g g a a a a a t t g t g a a g a t c t g t g a c t t t g g c c a c c g a c g a g c g t t g c a g g a g g a c c g t g t t c c t t t t t a a c a c t t c t a g a c a c t g a a a c c g g

BstBI 2760 LARDIMHDSNYVSKGSTFLP QGSVDHMIRVIHRFATGKQG t g g c c a g a g a c a t c a t g c a t g a t t c g a a c t a t g t g t c g a a a g g c a g t a c c t t t c t g c c c g a c c g g t c t c t g t a g t a c g t a c t a a g c t t g a t a c a c a g c t t t c c g t c a t g g a a a g a c g g g c

2820 VKWMAPESIFDNLYTTLSDV HLPHSRLADKVVEVGCQTID t g a a g t g g a t g g c t c c t g a g a g c a t c t t t g a c a a c c t c t a c a c c a c a c t g a g t g a t g t c t a c t t c a c c t a c c g a g g a c t c t c g t a g a a a c t g t t g g a g a t g t g g t g t g a c t c a c t a c a g a

BsmI 2880 WSYGILLWEIFSLGGTPYPG PRIANQEPLDKGKTAGRVGA g g t c t t a t g g c a t t c t g c t c t g g g a g a t c t t t t c c c t t g g t g g c a c c c c t t a c c c c g g c a c c a g a a t a c c g t a a g a c g a g a c c c t c t a g a a a a g g g a a c c a c c g t g g g g a a t g g g g c c g t

2940 MMVDSTFYNKIKSGYRMAKP HHHIRSEVILDLTPVPHGLR t g a t g g t g g a t t c t a c t t t c t a c a a t a a g a t c a a g a g t g g g t a c c g g a t g g c c a a g c c t g a c t a c c a c c t a a g a t g a a a g a t g t t a t t c t a g t t c t c a c c c a t g g c c t a c c g g t t c g g a c

AccI 3000 DHATSEVYEIMVKCWNSEPE VVSGTFDVLDHHFAPVTLRL a c c a c g c t a c c a g t g a a g t c t a c g a g a t c a t g g t g a a a t g c t g g a a c a g t g a g c c g g a g a t g g t g c g a t g g t c a c t t c a g a t g c t c t a g t a c c a c t t t a c g a c c t t g t c a c t c g g c c t c t

3060 KRPSFYHLSEIVENLLPGQY LSGGKVVQTLNHLIQQRSLI a g a g a c c c t c c t t t t a c c a c c t g a g t g a g a t t g t g g a g a a t c t g c t g c c t g g a c a a t a t a t c t c t g g g a g g a a a a t g g t g g a c t c a c t c t a a c a c c t c t t a g a c g a c g g a c c t g t t a t a t

3120 KKSYEKIHLDFLKSDHPAVA FLTIFFNVQVEQLTVM a a a a g a g t t a t g a a a a a a t t c a c c t g g a c t t c c t g a a g a g t g a c c a t c c t g c t g t g g c a c t t t t c t c a a t a c t t t t t t a a g t g g a c c t g a a g g a c t t c t c a c t g g t a g g a c g a c a c c g t g

3180 RMRVDSDNAYIGVTYKNEED g c a t g c g t g t g g a c t c a g a c a a t g c a t a c a t t g g t g t c a c c t a c a a a a a c g a g g a a g a c a c g t a c g c a c a c c t g a g t c t g t t a c g t a t g t a a c c a c a g t g g a t g t t t t t g c t c c t t c t g t

AfeI 3240 KLKDWEGGLDEQRLSADSGY a g c t g a a g g a c t g g g a g g g t g g t c t g g a t g a g c a g a g a c t g a g c g c t g a c a g t g g c t a c a t c g a c t t c c t g a c c c t c c c a c c a g a c c t a c t c g t c t c t g a c t c g c g a c t g t c a c c g a t g t

Bsu36I 3300 IIPLPDIDPVPEEEDLGKRN t c a t t c c t c t g c c t g a c a t t g a c c c t g t c c c t g a g g a g g a g g a c c t g g g c a a g a g g a a c a a g t a a g g a g a c g g a c t g t a a c t g g g a c a g g g a c t c c t c c t c c t g g a c c c g t t c t c c t t g t

BsmBIEsp3I 3360 RHSSQTSEESAIETGSSSST g a c a c a g c t c g c a g a c c t c t g a a g a g a g t g c c a t t g a g a c g g g t t c c a g c a g t t c c a c c t c t g t g t c g a g c g t c t g g a g a c t t c t c t c a c g g t a a c t c t g c c c a a g g t c g t c a a g g t g g a

3420 FIKREDETIEDIDMMDDIGI t c a t c a a g a g a g a g g a c g a g a c c a t t g a a g a c a t c g a c a t g a t g g a c g a c a t c g g c a t a g a g t a g t t c t c t c t c c t g c t c t g g t a a c t t c t g t a g c t g t a c t a c c t g c t g t a g c c g t a t c

SexAI*CsiI 3480 attL2 DSSDLVEDSFL* a c t c t t c a g a c c t g g t g g a a g a c a g c t t c c t g t a a t c a a c t t t c t t g t a c a a a g t t g g c a t g a g a a g t c t g g a c c a c c t t c t g t c g a a g g a c a t t a g t t g a a a g a a c a t g t t t c a a c c g t

3540 attL2 t t a t a g g a a a g c a t t g c t t a t c a a t t t g t t g c a a c g a a c a g g t c a c t a t c a g t c a a a a t a a a t a t c c t t t c g t a a c g a a t a g t t a a a c a a c g t t g c t t g t c c a g t g a t a g t c a g t t t t a t

HindIII 3600 attL2 a a a t c a t t a t t t g c a t g c a a g c t t a g a t c c t c t a g a c c a g c c a g g a c a g a a a t g c c t c g a t t t a g t a a t a a a c g t a c g t t c g a a t c t a g g a g a t c t g g t c g g t c c t g t c t t t a c g g a g c t

3660 c t t c g c t g c t a c c c a a g g t t g c c g g g t g a c g c a c a c c g t g g a a a c g g a t g a a g g c a c g a a g a a g c g a c g a t g g g t t c c a a c g g c c c a c t g c g t g t g g c a c c t t t g c c t a c t t c c g t g c t t

3720 MRS c c c a g t g g a c a t a a g c c t g t t c g g t t c g t a a g c t g t a a t g c a a g t a g c g t a t g c g c t c a c g g g t c a c c t g t a t t c g g a c a a g c c a a g c a t t c g a c a t t a c g t t c a t c g c a t a c g c g a g t g

3780 RNWSRTLTERSGGNGAVAVF g c a a c t g g t c c a g a a c c t t g a c c g a a c g c a g c g g t g g t a a c g g c g c a g t g g c g g t t t t c a c g t t g a c c a g g t c t t g g a a c t g g c t t g c g t c g c c a c c a t t g c c g c g t c a c c g c c a a a a g t

3840 MACYDCFFGVQSMPRASKQQ t g g c t t g t t a t g a c t g t t t t t t t g g g g t a c a g t c t a t g c c t c g g g c a t c c a a g c a g c a a g a c c g a a c a a t a c t g a c a a a a a a a c c c c a t g t c a g a t a c g g a g c c c g t a g g t t c g t c g t t c

3900 ARYAVGRCLMLWSSNDVTQQ c g c g t t a c g c c g t g g g t c g a t g t t t g a t g t t a t g g a g c a g c a a c g a t g t t a c g c a g c a g g g c g c a a t g c g g c a c c c a g c t a c a a a c t a c a a t a c c t c g t c g t t g c t a c a a t g c g t c g t c c

3960 1 5 10 MREAVIAEVS SmR GSRPKTKLNIMREAVIAEVS g c a g t c g c c c t a a a a c a a a g t t a a a c a t t a t g a g g g a a g c g g t g a t c g c c g a a g t a t c g a c g t c a g c g g g a t t t t g t t t c a a t t t g t a a t a c t c c c t t c g c c a c t a g c g g c t t c a t a g c t

4020 15 20 25 30 TQLSEVVGVIERHLEPTLLA SmR TQLSEVVGVIERHLEPTLLA c t c a a c t a t c a g a g g t a g t t g g c g t c a t c g a g c g c c a t c t c g a a c c g a c g t t g c t g g c c g g a g t t g a t a g t c t c c a t c a a c c g c a g t a g c t c g c g g t a g a g c t t g g c t g c a a c g a c c g g c

4080 35 40 45 50 VHLYGSAVDGGLKPHSDIDL SmR VHLYGSAVDGGLKPHSDIDL t a c a t t t g t a c g g c t c c g c a g t g g a t g g c g g c c t g a a g c c a c a c a g t g a t a t t g a t t t g c a t g t a a a c a t g c c g a g g c g t c a c c t a c c g c c g g a c t t c g g t g t g t c a c t a t a a c t a a a c g

4140 55 60 65 70 LVTVTVRLDETTRRALINDL SmR LVTVTVRLDETTRRALINDL t g g t t a c g g t g a c c g t a a g g c t t g a t g a a a c a a c g c g g c g a g c t t t g a t c a a c g a c c t t t a c c a a t g c c a c t g g c a t t c c g a a c t a c t t t g t t g c g c c g c t c g a a a c t a g t t g c t g g a a a

4200 75 80 85 90 LETSASPGESEILRAVEVTI SmR LETSASPGESEILRAVEVTI t g g a a a c t t c g g c t t c c c c t g g a g a g a g c g a g a t t c t c c g c g c t g t a g a a g t c a c c a t t g a c c t t t g a a g c c g a a g g g g a c c t c t c t c g c t c t a a g a g g c g c g a c a t c t t c a g t g g t a a c

4260 95 100 105 110 VVHDDIIPWRYPAKRELQFG SmR VVHDDIIPWRYPAKRELQFG t t g t g c a c g a c g a c a t c a t t c c g t g g c g t t a t c c a g c t a a g c g c g a a c t g c a a t t t g g a g a a c a c g t g c t g c t g t a g t a a g g c a c c g c a a t a g g t c g a t t c g c g c t t g a c g t t a a a c c t c

PvuI 4320 115 120 125 130 EWQRNDILAGIFEPATIDID SmR EWQRNDILAGIFEPATIDID a a t g g c a g c g c a a t g a c a t t c t t g c a g g t a t c t t c g a g c c a g c c a c g a t c g a c a t t g a t c t t a c c g t c g c g t t a c t g t a a g a a c g t c c a t a g a a g c t c g g t c g g t g c t a g c t g t a a c t a g

4380 135 140 145 150 LAILLTKAREHSVALVGPAA SmR LAILLTKAREHSVALVGPAA t g g c t a t c t t g c t g a c a a a a g c a a g a g a a c a t a g c g t t g c c t t g g t a g g t c c a g c g g c g g a c c g a t a g a a c g a c t g t t t t c g t t c t c t t g t a t c g c a a c g g a a c c a t c c a g g t c g c c g c c

4440 155 160 165 170 EELFDPVPEQDLFEALNETL SmR EELFDPVPEQDLFEALNETL a g g a a c t c t t t g a t c c g g t t c c t g a a c a g g a t c t a t t t g a g g c g c t a a a t g a a a c c t t a a t c c t t g a g a a a c t a g g c c a a g g a c t t g t c c t a g a t a a a c t c c g c g a t t t a c t t t g g a a t t

4500 175 180 185 190 TLWNSPPDWAGDERNVVLTL SmR TLWNSPPDWAGDERNVVLTL c g c t a t g g a a c t c g c c g c c c g a c t g g g c t g g c g a t g a g c g a a a t g t a g t g c t t a c g t t g t g c g a t a c c t t g a g c g g c g g g c t g a c c c g a c c g c t a c t c g c t t t a c a t c a c g a a t g c a a c a

4560 195 200 205 210 SRIWYSAVTGKIAPKDVAAD SmR SRIWYSAVTGKIAPKDVAAD c c c g c a t t t g g t a c a g c g c a g t a a c c g g c a a a a t c g c g c c g a a g g a t g t c g c t g c c g a c t g g g c g t a a a c c a t g t c g c g t c a t t g g c c g t t t t a g c g c g g c t t c c t a c a g c g a c g g c t g a

NgoMIVNaeI 4620 215 220 225 230 WAMERLPAQYQPVILEARQA SmR WAMERLPAQYQPVILEARQA g g g c a a t g g a g c g c c t g c c g g c c c a g t a t c a g c c c g t c a t a c t t g a a g c t a g a c a g g c t t c c c g t t a c c t c g c g g a c g g c c g g g t c a t a g t c g g g c a g t a t g a a c t t c g a t c t g t c c g a a

BssHII 4680 235 240 245 250 YLGQEEDRLASRADQLEEFV SmR YLGQEEDRLASRADQLEEFV a t c t t g g a c a a g a a g a a g a t c g c t t g g c c t c g c g c g c a g a t c a g t t g g a a g a a t t t g t c c t a g a a c c t g t t c t t c t t c t a g c g a a c c g g a g c g c g c g t c t a g t c a a c c t t c t t a a a c a g g

PaeR7IPspXIXhoIBsaAI 4740 255 260 HYVKGEITKVVGK* SmR HYVKGEITKVVGK* a c t a c g t g a a a g g c g a g a t c a c c a a g g t a g t c g g c a a a t a a c c c t c g a g c c a c c c a t g a c t g a t g c a c t t t c c g c t c t a g t g g t t c c a t c a g c c g t t t a t t g g g a g c t c g g t g g g t a c t g

4800 c a a a a t c c c t t a a c g t g a g t t a c g c g t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a g t t t t a g g g a a t t g c a c t c a a t g c g c a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t

4860 ori t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t

4920 ori a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t

4980 ori a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a

5040 ori t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a

5100 ori t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a

5160 ori a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a

5220 ori t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c a t t g a g a a a g c g c c a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g t a a c t c t t t c g c g g t

BciVI 5280 ori c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c

BssSαIBssSI 5340 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g

5400 ori g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t

PciI 5460 ori a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t

5520 t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c

5580 A G C G A G T C A G T G A G C G A G L4440 c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c

5640 a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a

5700 g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t a c g c g t a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a t g c g c a t

5760 rrnB T2 terminator c c g c t a g c c a g g a a g a g t t t g t a g a a a c g c a a a a a g g c c a t c c g t c a g g a t g g c c t t c t g g g c g a t c g g t c c t t c t c a a a c a t c t t t g c g t t t t t c c g g t a g g c a g t c c t a c c g g a a g a c

5820 c t t a g t t t g a t g c c t g g c a g t t t a t g g c g g g c g t c c t g c c c g c c a c c c t c c g g g c c g t t g g a a t c a a a c t a c g g a c c g t c a a a t a c c g c c c g c a g g a c g g g c g g t g g g a g g c c c g g c a a c

5880 rrnB T1 terminator c t t c a c a a c g t t c a a a t c c g c t c c c g g c g g a t t t g t c c t a c t c a g g a g a g c g t t c a c c g a g a a g t g t t g c a a g t t t a g g c g a g g g c c g c c t a a a c a g g a t g a g t c c t c t c g c a a g t g g c t

5940 rrnB T1 terminator c a a a c a a c a g a t a a a a c g a a a g g c c c a g t c t t c c g a c t g a g c c t t t c g t t t t a t t t g a t g g t t t g t t g t c t a t t t t g c t t t c c g g g t c a g a a g g c t g a c t c g g a a a g c a a a a t a a a c t a c

5963 c c t g g c a g t t c c c t a c t c t c g c g g g a c c g t c a a g g g a t g a g a g c g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.