Addgene R777-E127 Hs.MAPK3 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: R777-E127 Hs.MAPK3


Map View

BbsI (3771) AclI (3698) rrnB T2 terminator BspQI - SapI (3445) L4440 (3426 .. 3443) PciI (3328) DrdI (3226) pBR322ori-F (3173 .. 3192) BssSI - BssSαI (3155) BciVI (3130) PspFI (3028) BseYI (3024) PaeR7I - PspXI - XhoI (2594) BssHII (2522) NaeI (2449) NgoMIV (2447) BglI (2430) BtgZI (2356) PvuI (2179) BclI * (1996) M13/pUC Forward (23 .. 45) M13 Forward (37 .. 54) RsrII (71) BamHI (78) PsiI (153) Kozak sequence KflI (254) BmgBI (296) BsiWI (340) PmlI (371) FspI (375) MscI (390) BstBI (412) BsaBI * (453) AccI (538) BsgI (567) BglII (680) AflII - PaqCI (723) Acc65I (811) KpnI (815) BsmI (883) PflMI (956) XcmI (1049) AfeI (1156) ScaI (1182) BseRI (1227) BstXI (1238) EcoNI (1251) Bsu36I (1252) SphI (1427) XbaI (1441) BstEII (1958) R777-E127 Hs.MAPK3 3833 bp

Sequence View

60 M13 fwd C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward t t a a c g c t a g c a t g g a t g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t c t t a a a a a t t g c g a t c g t a c c t a c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a g a a t t t

BamHIRsrII 120 attL1 *KSQYHGT a t t c g a g c t c g g t c c g a g g a t c c g a a a t a a t g a t t t t a t t t t g a c t g a t a g t g a c c t g t t t a a g c t c g a g c c a g g c t c c t a g g c t t t a t t a c t a a a a t a a a a c t g a c t a t c a c t g g a c a a

PsiI 180 attL1 MPTLYKKV RQLLNILLAKKYHWSQVFFN c g t t g c a a c a a a t t g a t a a g c a a t g c t t t t t t a t a a t g c c a a c t t t g t a c a a a a a a g t t g g c a a c g t t g t t t a a c t a t t c g t t a c g a a a a a a t a t t a c g g t t g a a a c a t g t t t t t t c a a c

240 attL1 Kozak sequence GTMAAAAAQGGGGGEPRRTE AGHRRRRSLPAPAPLGTSGL g c a c c a t g g c g g c g g c g g c g g c t c a g g g g g g c g g g g g c g g g g a g c c c c g t a g a a c c g a g g c g t g g t a c c g c c g c c g c c g c c g a g t c c c c c c g c c c c c g c c c c t c g g g g c a t c t t g g c t c c

BmgBIKflI 300 GVGPGVPGEVEMVKGQPFDV PDARPDRPLHLHHLPLREVH g g g t c g g c c c g g g g g t c c c g g g g g a g g t g g a g a t g g t g a a g g g g c a g c c g t t c g a c g t g g c c c a g c c g g g c c c c c a g g g c c c c c t c c a c c t c t a c c a c t t c c c c g t c g g c a a g c t g c a c c

BsiWI 360 GPRYTQLQYIGEGAYGMVSS ARAVRLQLVDALARVAHDAR g c c c g c g c t a c a c g c a g t t g c a g t a c a t c g g c g a g g g c g c g t a c g g c a t g g t c a g c t c g g c g g g c g c g a t g t g c g t c a a c g t c a t g t a g c c g c t c c c g c g c a t g c c g t a c c a g t c g a g c c

BstBIMscIFspIPmlI 420 AYDHVRKTRVAIKKISPFEH GIVVHALSAHGDLLDAGEFM c c t a t g a c c a c g t g c g c a a g a c t c g c g t g g c c a t c a a g a a g a t c a g c c c c t t c g a a c a t c g g a t a c t g g t g c a c g c g t t c t g a g c g c a c c g g t a g t t c t t c t a g t c g g g g a a g c t t g t a g

BsaBI* 480 QTYCQRTLREIQILLRFRHE LGVALAREPLDLDQQAEAML a g a c c t a c t g c c a g c g c a c g c t c c g g g a g a t c c a g a t c c t g c t g c g c t t c c g c c a t g a g a t c t g g a t g a c g g t c g c g t g c g a g g c c c t c t a g g t c t a g g a c g a c g c g a a g g c g g t a c t c t

AccI 540 NVIGIRDILRASTLEAMRDV IDDADSVNQPRGGQFGHSID a t g t c a t c g g c a t c c g a g a c a t t c t g c g g g c g t c c a c c c t g g a a g c c a t g a g a g a t g t c t t a c a g t a g c c g t a g g c t c t g t a a g a c g c c c g c a g g t g g g a c c t t c g g t a c t c t c t a c a g a

BsgI 600 YIVQDLMETDLYKLLKSQQL VNHLVQHLSVQVLQQFALLQ a c a t t g t g c a g g a c c t g a t g g a g a c t g a c c t g t a c a a g t t g c t g a a a a g c c a g c a g c t g a t g t a a c a c g t c c t g g a c t a c c t c t g a c t g g a c a t g t t c a a c g a c t t t t c g g t c g t c g a c t

660 SNDHICYFLYQILRGLKYIH AIVM g c a a t g a c c a t a t c t g c t a c t t c c t c t a c c a g a t c c t g c g g g g c c t c a a g t a c a t c c a c t c g t t a c t g g t a t a g a c g a t g a a g g a g a t g g t c t a g g a c g c c c c g g a g t t c a t g t a g g t g a

BglII 720 SANVLHRDLKPSNLLINTTC c c g c c a a c g t g c t c c a c c g a g a t c t a a a g c c c t c c a a c c t g c t c a t c a a c a c c a c c t g c g g g c g g t t g c a c g a g g t g g c t c t a g a t t t c g g g a g g t t g g a c g a g t a g t t g t g g t g g a c g c

PaqCIAflII 780 DLKICDFGLARIADPEHDHT a c c t t a a g a t t t g t g a t t t c g g c c t g g c c c g g a t t g c c g a t c c t g a g c a t g a c c a c a c c g t g g a a t t c t a a a c a c t a a a g c c g g a c c g g g c c t a a c g g c t a g g a c t c g t a c t g g t g t g g c

Acc65IKpnI 840 GFLTEYVATRWYRAPEIMLN g c t t c c t g a c g g a g t a t g t g g c t a c g c g c t g g t a c c g g g c c c c a g a g a t c a t g c t g a a c t c g a a g g a c t g c c t c a t a c a c c g a t g c g c g a c c a t g g c c c g g g g t c t c t a g t a c g a c t t g a

BsmI 900 SKGYTKSIDIWSVGCILAEM c c a a g g g c t a t a c c a a g t c c a t c g a c a t c t g g t c t g t g g g c t g c a t t c t g g c t g a g a t g c g g t t c c c g a t a t g g t t c a g g t a g c t g t a g a c c a g a c a c c c g a c g t a a g a c c g a c t c t a c g

PflMI 960 LSNRPIFPGKHYLDQLNHIL t c t c t a a c c g g c c c a t c t t c c c t g g c a a g c a c t a c c t g g a t c a g c t c a a c c a c a t t c t g g a g a g a t t g g c c g g g t a g a a g g g a c c g t t c g t g a t g g a c c t a g t c g a g t t g g t g t a a g a c c

1020 GILGSPSQEDLNCIINMKAR g c a t c c t g g g c t c c c c a t c c c a g g a g g a c c t g a a t t g t a t c a t c a a c a t g a a g g c c c g a a c g t a g g a c c c g a g g g g t a g g g t c c t c c t g g a c t t a a c a t a g t a g t t g t a c t t c c g g g c t t

XcmI 1080 NYLQSLPSKTKVAWAKLFPK a c t a c c t a c a g t c t c t g c c c t c c a a g a c c a a g g t g g c t t g g g c c a a g c t t t t c c c c a a g t t g a t g g a t g t c a g a g a c g g g a g g t t c t g g t t c c a c c g a a c c c g g t t c g a a a a g g g g t t c a

1140 SDSKALDLLDRMLTFNPNKR c a g a c t c c a a a g c c c t t g a c c t g c t g g a c c g g a t g t t a a c c t t t a a c c c c a a t a a a c g g a g t c t g a g g t t t c g g g a a c t g g a c g a c c t g g c c t a c a a t t g g a a a t t g g g g t t a t t t g c c t

ScaIAfeI 1200 ITVEEALAHPYLEQYYDPTD t c a c a g t g g a g g a a g c g c t g g c t c a c c c c t a c c t g g a g c a g t a c t a t g a c c c g a c g g a t g a g t g t c a c c t c c t t c g c g a c c g a g t g g g g a t g g a c c t c g t c a t g a t a c t g g g c t g c c t a c

EcoNIBsu36IBstXIBseRI 1260 EPVAEEPFTFAMELDDLPKE a g c c a g t g g c c g a g g a g c c c t t c a c c t t c g c c a t g g a g c t g g a t g a c c t a c c t a a g g a g c t c g g t c a c c g g c t c c t c g g g a a g t g g a a g c g g t a c c t c g a c c t a c t g g a t g g a t t c c t c g

1320 RLKELIFQETARFQPGVLEA g g c t g a a g g a g c t c a t c t t c c a g g a g a c a g c a c g c t t c c a g c c c g g a g t g c t g g a g g c c c c c g a c t t c c t c g a g t a g a a g g t c c t c t g t c g t g c g a a g g t c g g g c c t c a c g a c c t c c g g g

1380 attL2 P* c c t a a t c a a c t t t c t t g t a c a a a g t t g g c a t t a t a g g a a a g c a t t g c t t a t c a a t t t g t t g g a t t a g t t g a a a g a a c a t g t t t c a a c c g t a a t a t c c t t t c g t a a c g a a t a g t t a a a c a a

SphI 1440 attL2 g c a a c g a a c a g g t c a c t a t c a g t c a a a a t a a a a t c a t t a t t t g c a t g c a a g c t t a g a t c c c g t t g c t t g t c c a g t g a t a g t c a g t t t t a t t t t a g t a a t a a a c g t a c g t t c g a a t c t a g g

XbaI 1500 t c t a g a c c a g c c a g g a c a g a a a t g c c t c g a c t t c g c t g c t a c c c a a g g t t g c c g g g t g a c a g a t c t g g t c g g t c c t g t c t t t a c g g a g c t g a a g c g a c g a t g g g t t c c a a c g g c c c a c t g

1560 g c a c a c c g t g g a a a c g g a t g a a g g c a c g a a c c c a g t g g a c a t a a g c c t g t t c g g t t c g t a c g t g t g g c a c c t t t g c c t a c t t c c g t g c t t g g g t c a c c t g t a t t c g g a c a a g c c a a g c a t

1620 MRSRNWSRTLTER a g c t g t a a t g c a a g t a g c g t a t g c g c t c a c g c a a c t g g t c c a g a a c c t t g a c c g a a c g c a t c g a c a t t a c g t t c a t c g c a t a c g c g a g t g c g t t g a c c a g g t c t t g g a a c t g g c t t g c g t

1680 SGGNGAVAVFMACYDCFFGV g c g g t g g t a a c g g c g c a g t g g c g g t t t t c a t g g c t t g t t a t g a c t g t t t t t t t g g g g t a c c g c c a c c a t t g c c g c g t c a c c g c c a a a a g t a c c g a a c a a t a c t g a c a a a a a a a c c c c a t g

1740 QSMPRASKQQARYAVGRCLM a g t c t a t g c c t c g g g c a t c c a a g c a g c a a g c g c g t t a c g c c g t g g g t c g a t g t t t g a t g t t c a g a t a c g g a g c c c g t a g g t t c g t c g t t c g c g c a a t g c g g c a c c c a g c t a c a a a c t a c a

1800 SmR LWSSNDVTQQGSRPKTKLNI t a t g g a g c a g c a a c g a t g t t a c g c a g c a g g g c a g t c g c c c t a a a a c a a a g t t a a a c a t t a a t a c c t c g t c g t t g c t a c a a t g c g t c g t c c c g t c a g c g g g a t t t t g t t t c a a t t t g t a a t

1860 1 5 10 15 20 MREAVIAEVSTQLSEVVGVI SmR MREAVIAEVSTQLSEVVGVI t g a g g g a a g c g g t g a t c g c c g a a g t a t c g a c t c a a c t a t c a g a g g t a g t t g g c g t c a t c g a c t c c c t t c g c c a c t a g c g g c t t c a t a g c t g a g t t g a t a g t c t c c a t c a a c c g c a g t a g c

1920 25 30 35 40 ERHLEPTLLAVHLYGSAVDG SmR ERHLEPTLLAVHLYGSAVDG a g c g c c a t c t c g a a c c g a c g t t g c t g g c c g t a c a t t t g t a c g g c t c c g c a g t g g a t g g c g t c g c g g t a g a g c t t g g c t g c a a c g a c c g g c a t g t a a a c a t g c c g a g g c g t c a c c t a c c g c

BstEII 1980 45 50 55 60 GLKPHSDIDLLVTVTVRLDE SmR GLKPHSDIDLLVTVTVRLDE g c c t g a a g c c a c a c a g t g a t a t t g a t t t g c t g g t t a c g g t g a c c g t a a g g c t t g a t g a a a c g g a c t t c g g t g t g t c a c t a t a a c t a a a c g a c c a a t g c c a c t g g c a t t c c g a a c t a c t t t

BclI* 2040 65 70 75 80 TTRRALINDLLETSASPGES SmR TTRRALINDLLETSASPGES c a a c g c g g c g a g c t t t g a t c a a c g a c c t t t t g g a a a c t t c g g c t t c c c c t g g a g a g a g c g g t t g c g c c g c t c g a a a c t a g t t g c t g g a a a a c c t t t g a a g c c g a a g g g g a c c t c t c t c g c

2100 85 90 95 100 EILRAVEVTIVVHDDIIPWR SmR EILRAVEVTIVVHDDIIPWR a g a t t c t c c g c g c t g t a g a a g t c a c c a t t g t t g t g c a c g a c g a c a t c a t t c c g t g g c g t t t c t a a g a g g c g c g a c a t c t t c a g t g g t a a c a a c a c g t g c t g c t g t a g t a a g g c a c c g c a a

2160 105 110 115 120 YPAKRELQFGEWQRNDILAG SmR YPAKRELQFGEWQRNDILAG a t c c a g c t a a g c g c g a a c t g c a a t t t g g a g a a t g g c a g c g c a a t g a c a t t c t t g c a g g t a t a g g t c g a t t c g c g c t t g a c g t t a a a c c t c t t a c c g t c g c g t t a c t g t a a g a a c g t c c a t

PvuI 2220 125 130 135 140 IFEPATIDIDLAILLTKARE SmR IFEPATIDIDLAILLTKARE t c t t c g a g c c a g c c a c g a t c g a c a t t g a t c t g g c t a t c t t g c t g a c a a a a g c a a g a g a a c a g a a g c t c g g t c g g t g c t a g c t g t a a c t a g a c c g a t a g a a c g a c t g t t t t c g t t c t c t t g

2280 145 150 155 160 HSVALVGPAAEELFDPVPEQ SmR HSVALVGPAAEELFDPVPEQ a t a g c g t t g c c t t g g t a g g t c c a g c g g c g g a g g a a c t c t t t g a t c c g g t t c c t g a a c a g g t a t c g c a a c g g a a c c a t c c a g g t c g c c g c c t c c t t g a g a a a c t a g g c c a a g g a c t t g t c c

2340 165 170 175 180 DLFEALNETLTLWNSPPDWA SmR DLFEALNETLTLWNSPPDWA a t c t a t t t g a g g c g c t a a a t g a a a c c t t a a c g c t a t g g a a c t c g c c g c c c g a c t g g g c t g t a g a t a a a c t c c g c g a t t t a c t t t g g a a t t g c g a t a c c t t g a g c g g c g g g c t g a c c c g a c

BtgZI 2400 185 190 195 200 GDERNVVLTLSRIWYSAVTG SmR GDERNVVLTLSRIWYSAVTG g c g a t g a g c g a a a t g t a g t g c t t a c g t t g t c c c g c a t t t g g t a c a g c g c a g t a a c c g g c a c g c t a c t c g c t t t a c a t c a c g a a t g c a a c a g g g c g t a a a c c a t g t c g c g t c a t t g g c c g t

NgoMIVNaeIBglI 2460 205 210 215 220 KIAPKDVAADWAMERLPAQY SmR KIAPKDVAADWAMERLPAQY a a a t c g c g c c g a a g g a t g t c g c t g c c g a c t g g g c a a t g g a g c g c c t g c c g g c c c a g t a t c t t t a g c g c g g c t t c c t a c a g c g a c g g c t g a c c c g t t a c c t c g c g g a c g g c c g g g t c a t a g

2520 225 230 235 240 QPVILEARQAYLGQEEDRLA SmR QPVILEARQAYLGQEEDRLA a g c c c g t c a t a c t t g a a g c t a g a c a g g c t t a t c t t g g a c a a g a a g a a g a t c g c t t g g c c t t c g g g c a g t a t g a a c t t c g a t c t g t c c g a a t a g a a c c t g t t c t t c t t c t a g c g a a c c g g a

BssHII 2580 245 250 255 260 SRADQLEEFVHYVKGEITKV SmR SRADQLEEFVHYVKGEITKV c g c g c g c a g a t c a g t t g g a a g a a t t t g t c c a c t a c g t g a a a g g c g a g a t c a c c a a g g t a g g c g c g c g t c t a g t c a a c c t t c t t a a a c a g g t g a t g c a c t t t c c g c t c t a g t g g t t c c a t c

PaeR7IPspXIXhoI 2640 VGK* SmR VGK* t c g g c a a a t a a c c c t c g a g c c a c c c a t g a c c a a a a t c c c t t a a c g t g a g t t a c g c g t c g t a g c c g t t t a t t g g g a g c t c g g t g g g t a c t g g t t t t a g g g a a t t g c a c t c a a t g c g c a g c a

2700 ori t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g

2760 ori t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g

2820 ori c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g

2880 ori c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g

2940 ori c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a

3000 ori c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a

BseYIPspFI 3060 ori g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a

3120 ori a c c t a c a g c g t g a g c a t t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t t g g a t g t c g c a c t c g t a a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a

BssSαIBssSIBciVI 3180 ori G G G A A A C G pBR322ori-F a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c

DrdI 3240 ori C C T G G T A T C T T T pBR322ori-F c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a

3300 ori g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a

PciI 3360 t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c

3420 t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c

BspQISapI 3480 A G C G A G T C A G T G A G C G A G L4440 a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g

3540 c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c

3600 g c a g t g a g c g c a a c g c a a t t a a t a c g c g t a c c g c t a g c c a g g a a g a g t t t g t a g a a a c g c c g t c a c t c g c g t t g c g t t a a t t a t g c g c a t g g c g a t c g g t c c t t c t c a a a c a t c t t t g c g

3660 rrnB T2 terminator a a a a a g g c c a t c c g t c a g g a t g g c c t t c t g c t t a g t t t g a t g c c t g g c a g t t t a t g g c g g t t t t t c c g g t a g g c a g t c c t a c c g g a a g a c g a a t c a a a c t a c g g a c c g t c a a a t a c c g c c

AclI 3720 g c g t c c t g c c c g c c a c c c t c c g g g c c g t t g c t t c a c a a c g t t c a a a t c c g c t c c c g g c g g c g c a g g a c g g g c g g t g g g a g g c c c g g c a a c g a a g t g t t g c a a g t t t a g g c g a g g g c c g c c

BbsI 3780 rrnB T1 terminator a t t t g t c c t a c t c a g g a g a g c g t t c a c c g a c a a a c a a c a g a t a a a a c g a a a g g c c c a g t c t a a a c a g g a t g a g t c c t c t c g c a a g t g g c t g t t t g t t g t c t a t t t t g c t t t c c g g g t c a g

3833 rrnB T1 terminator t t c c g a c t g a g c c t t t c g t t t t a t t t g a t g c c t g g c a g t t c c c t a c t c t c g c g a a g g c t g a c t c g g a a a g c a a a a t a a a c t a c g g a c c g t c a a g g g a t g a g a g c g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.