Addgene R777-E090 Hs.FGFR4-nostop - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: R777-E090 Hs.FGFR4-nostop


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HincII - HpaI (2) AclI (4964) rrnB T2 terminator L4440 (4692 .. 4709) PciI (4594) DrdI (4492) pBR322ori-F (4439 .. 4458) PaeR7I - PspXI - XhoI (3860) BssHII (3788) PvuI (3445) BclI * (3262) BstEII (3224) HindIII (2695) M13/pUC Forward (23 .. 45) M13 Forward (37 .. 54) Eco53kI (67) SacI (69) PsiI (153) Bsu36I (463) KflI (587) AflII (738) BsmI (781) SbfI (940) XcmI (968) NmeAIII (1183) AccI (1284) BstZ17I (1285) TspMI - XmaI (1573) SmaI (1575) AvrII (1601) BbvCI - Bpu10I (1899) PfoI (1998) R777-E090 Hs.FGFR4-nostop 5099 bp

Sequence View

HincIIHpaI 60 M13 fwd C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward t t a a c g c t a g c a t g g a t g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t c t t a a a a a t t g c g a t c g t a c c t a c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a g a a t t t

Eco53kISacI 120 attL1 a t t c g a g c t c g g t c c g a g g a t c c g a a a t a a t g a t t t t a t t t t g a c t g a t a g t g a c c t g t t t a a g c t c g a g c c a g g c t c c t a g g c t t t a t t a c t a a a a t a a a a c t g a c t a t c a c t g g a c a a

PsiI 180 attL1 MPTLYKKV c g t t g c a a c a a a t t g a t a a g c a a t g c t t t t t t a t a a t g c c a a c t t t g t a c a a a a a a g t t g g c a a c g t t g t t t a a c t a t t c g t t a c g a a a a a a t a t t a c g g t t g a a a c a t g t t t t t t c a a c

240 attL1 GTMRLLLALLGVLLSVPGPP g c a c c a t g c g g c t g c t g c t g g c c c t g t t g g g g g t c c t g c t g a g t g t g c c t g g g c c t c c a g c g t g g t a c g c c g a c g a c g a c c g g g a c a a c c c c c a g g a c g a c t c a c a c g g a c c c g g a g g t c

300 VLSLEASEEVELEPCLAPSL t c t t g t c c c t g g a g g c c t c t g a g g a a g t g g a g c t t g a g c c c t g c c t g g c t c c c a g c c t g g a g a a c a g g g a c c t c c g g a g a c t c c t t c a c c t c g a a c t c g g g a c g g a c c g a g g g t c g g a c c

360 EQQEQELTVALGQPVRLCCG a g c a g c a a g a g c a g g a g c t g a c a g t a g c c c t t g g g c a g c c t g t g c g t c t g t g c t g t g g g c t c g t c g t t c t c g t c c t c g a c t g t c a t c g g g a a c c c g t c g g a c a c g c a g a c a c g a c a c c c g

420 RAERGGHWYKEGSRLAPAGR g g g c t g a g c g t g g t g g c c a c t g g t a c a a g g a g g g c a g t c g c c t g g c a c c t g c t g g c c g t g c c c g a c t c g c a c c a c c g g t g a c c a t g t t c c t c c c g t c a g c g g a c c g t g g a c g a c c g g c a c

Bsu36I 480 VRGWRGRLEIASFLPEDAGR t a c g g g g c t g g a g g g g c c g c c t a g a g a t t g c c a g c t t c c t a c c t g a g g a t g c t g g c c g c t a t g c c c c g a c c t c c c c g g c g g a t c t c t a a c g g t c g a a g g a t g g a c t c c t a c g a c c g g c g a

540 YLCLARGSMIVLQNLTLITG a c c t c t g c c t g g c a c g a g g c t c c a t g a t c g t c c t g c a g a a t c t c a c c t t g a t t a c a g g t g t g g a g a c g g a c c g t g c t c c g a g g t a c t a g c a g g a c g t c t t a g a g t g g a a c t a a t g t c c a c

KflI 600 DSLTSSNDDEDPKSHRDPSN a c t c c t t g a c c t c c a g c a a c g a t g a t g a g g a c c c c a a g t c c c a t a g g g a c c c c t c g a a t a t g a g g a a c t g g a g g t c g t t g c t a c t a c t c c t g g g g t t c a g g g t a t c c c t g g g g a g c t t a t

660 RHSYPQQAPYWTHPQRMEKK g g c a c a g t t a c c c c c a g c a a g c a c c c t a c t g g a c a c a c c c c c a g c g c a t g g a g a a g a a a c c c g t g t c a a t g g g g g t c g t t c g t g g g a t g a c c t g t g t g g g g g t c g c g t a c c t c t t c t t t g

720 LHAVPAGNTVKFRCPAAGNP t g c a t g c a g t a c c t g c g g g g a a c a c c g t c a a g t t c c g c t g t c c a g c t g c a g g c a a c c c c a a c g t a c g t c a t g g a c g c c c c t t g t g g c a g t t c a a g g c g a c a g g t c g a c g t c c g t t g g g g t

AflII 780 TPTIRWLKDGQAFHGENRIG c g c c c a c c a t c c g c t g g c t t a a g g a t g g a c a g g c c t t t c a t g g g g a g a a c c g c a t t g g a g g c g g g t g g t a g g c g a c c g a a t t c c t a c c t g t c c g g a a a g t a c c c c t c t t g g c g t a a c c t c

BsmI 840 GIRLRHQHWSLVMESVVPSD g c a t t c g g c t g c g c c a t c a g c a c t g g a g t c t c g t g a t g g a g a g c g t g g t g c c c t c g g a c c c g t a a g c c g a c g c g g t a g t c g t g a c c t c a g a g c a c t a c c t c t c g c a c c a c g g g a g c c t g g

900 RGTYTCLVENAVGSIRYNYL g c g g c a c a t a c a c c t g c c t g g t a g a g a a c g c t g t g g g c a g c a t c c g c t a t a a c t a c c t g c c g c c g t g t a t g t g g a c g g a c c a t c t c t t g c g a c a c c c g t c g t a g g c g a t a t t g a t g g a c g

SbfI 960 LDVLERSPHRPILQAGLPAN t a g a t g t g c t g g a g c g g t c c c c g c a c c g g c c c a t c c t g c a g g c c g g g c t c c c g g c c a a c a a t c t a c a c g a c c t c g c c a g g g g c g t g g c c g g g t a g g a c g t c c g g c c c g a g g g c c g g t t g t

XcmI 1020 TTAVVGSDVELLCKVYSDAQ c c a c a g c c g t g g t g g g c a g c g a c g t g g a g c t g c t g t g c a a g g t g t a c a g c g a t g c c c a g c g g t g t c g g c a c c a c c c g t c g c t g c a c c t c g a c g a c a c g t t c c a c a t g t c g c t a c g g g t c g

1080 PHIQWLKHIVINGSSFGADG c c c a c a t c c a g t g g c t g a a g c a c a t c g t c a t c a a c g g c a g c a g c t t c g g a g c c g a c g g t t g g g t g t a g g t c a c c g a c t t c g t g t a g c a g t a g t t g c c g t c g t c g a a g c c t c g g c t g c c a a

1140 FPYVQVLKTADINSSEVEVL t c c c c t a t g t g c a a g t c c t a a a g a c t g c a g a c a t c a a t a g c t c a g a g g t g g a g g t c c t g t a g g g g a t a c a c g t t c a g g a t t t c t g a c g t c t g t a g t t a t c g a g t c t c c a c c t c c a g g a c a

NmeAIII 1200 YLRNVSAEDAGEYTCLAGNS a c c t g c g g a a c g t g t c a g c c g a g g a c g c a g g c g a g t a c a c c t g c c t c g c a g g c a a t t c c a t g g a c g c c t t g c a c a g t c g g c t c c t g c g t c c g c t c a t g t g g a c g g a g c g t c c g t t a a g g t

1260 IGLSYQSAWLTVLPEEDPTW *WDAQSVTSGSSSGVHV t c g g c c t c t c c t a c c a g t c t g c c t g g c t c a c g g t g c t g c c a g a g g a g g a c c c c a c a t g g a a g c c g g a g a g g a t g g t c a g a c g g a c c g a g t g c c a c g a c g g t c t c c t c c t g g g g t g t a c c t

AccIBstZ17I 1320 TAAAPEARYTDIILYASGSL AAAGSALYVSMMRYADPERA c c g c a g c a g c g c c c g a g g c c a g g t a t a c g g a c a t c a t c c t g t a c g c g t c g g g c t c c c t g g g g c g t c g t c g c g g g c t c c g g t c c a t a t g c c t g t a g t a g g a c a t g c g c a g c c c g a g g g a c c

1380 1 IEGR RSTAG Factor Xa site ALAVLLLLAGLYRGQALHGR KATSRSSAPSYRPCASWPRC c c t t g g c t g t g c t c c t g c t g c t g g c c g g g c t g t a t c g a g g g c a g g c g c t c c a c g g c c g g c g g a a c c g a c a c g a g g a c g a c g a c c g g c c c g a c a t a g c t c c c g t c c g c g a g g t g c c g g c c g

1440 (in frame with Factor Xa site) TPARPPLCRSSPASLWPDSS HPRPPATVQKLSRFPLARQF GRGGAVTCFSERKGRARCNE a c c c c c g c c c g c c c g c c a c t g t g c a g a a g c t c t c c c g c t t c c c t c t g g c c c g a c a g t t c t t g g g g g c g g g c g g g c g g t g a c a c g t c t t c g a g a g g g c g a a g g g a g a c c g g g c t g t c a a g a

1500 (in frame with Factor Xa site) PWSQALPASQAHPWYEACVS SLESGSSGKSSSSLVRGVRL RSDPEEPLDLEDRTRPTRRE c c c t g g a g t c a g g c t c t t c c g g c a a g t c a a g c t c a t c c c t g g t a c g a g g c g t g c g t c t c t g g g a c c t c a g t c c g a g a a g g c c g t t c a g t t c g a g t a g g g a c c a t g c t c c g c a c g c a g a g a

1560 (in frame with Factor Xa site) PPAAPPCSPAS* SSSGPALLAGLVSLDLPLDP ELPGAKSAPRTLRSRGRSGS c c t c c a g c g g c c c c g c c t t g c t c g c c g g c c t c g t g a g t c t a g a t c t a c c t c t c g a c c c a c g g a g g t c g c c g g g g c g g a a c g a g c g g c c g g a g c a c t c a g a t c t a g a t g g a g a g c t g g g t g

AvrIITspMIXmaISmaI 1620 LWEFPRDRLVLGKPLGEGCF HSNGRSLSTSPFGRPSPQKP t a t g g g a g t t c c c c c g g g a c a g g c t g g t g c t t g g g a a g c c c c t a g g c g a g g g c t g c t t t g a t a c c c t c a a g g g g g c c c t g t c c g a c c a c g a a c c c t t c g g g g a t c c g c t c c c g a c g a a a c

1680 GQVVRAEAFGMDPARPDQAS WTTRASAKPMSGARGSWALV g c c a g g t a g t a c g t g c a g a g g c c t t t g g c a t g g a c c c t g c c c g g c c t g a c c a a g c c a g c a c g g t c c a t c a t g c a c g t c t c c g g a a a c c g t a c c t g g g a c g g g c c g g a c t g g t t c g g t c g t

1740 TVAVKMLKDNASDKDLADLV TATLISLSLAESLSRASRTE c t g t g g c c g t c a a g a t g c t c a a a g a c a a c g c c t c t g a c a a g g a c c t g g c c g a c c t g g t c t g a c a c c g g c a g t t c t a c g a g t t t c t g t t g c g g a g a c t g t t c c t g g a c c g g c t g g a c c a g a

1800 SEMEVMKLIGRHKNIINLLG SISTIFSIPRCLFMM c g g a g a t g g a g g t g a t g a a g c t g a t c g g c c g a c a c a a g a a c a t c a t c a a c c t g c t t g g t g g c c t c t a c c t c c a c t a c t t c g a c t a g c c g g c t g t g t t c t t g t a g t a g t t g g a c g a a c c a c

1860 VCTQEGPLYVIVECAAKGNL t c t g c a c c c a g g a a g g g c c c c t g t a c g t g a t c g t g g a g t g c g c c g c c a a g g g a a a c c t g c a g a c g t g g g t c c t t c c c g g g g a c a t g c a c t a g c a c c t c a c g c g g c g g t t c c c t t t g g a c g

Bpu10IBbvCI 1920 REFLRARRPPGPDLSPDGPR g g g a g t t c c t g c g g g c c c g g c g c c c c c c a g g c c c c g a c c t c a g c c c c g a c g g t c c t c g g a c c c t c a a g g a c g c c c g g g c c g c g g g g g g t c c g g g g c t g g a g t c g g g g c t g c c a g g a g c c t

1980 SSEGPLSFPVLVSCAYQVAR g c a g t g a g g g g c c g c t c t c c t t c c c a g t c c t g g t c t c c t g c g c c t a c c a g g t g g c c c g a g c g t c a c t c c c c g g c g a g a g g a a g g g t c a g g a c c a g a g g a c g c g g a t g g t c c a c c g g g c t c

PfoI 2040 GMQYLESRKCIHRDLAARNV g c a t g c a g t a t c t g g a g t c c c g g a a g t g t a t c c a c c g g g a c c t g g c t g c c c g c a a t g t g c c g t a c g t c a t a g a c c t c a g g g c c t t c a c a t a g g t g g c c c t g g a c c g a c g g g c g t t a c a c g

2100 LVTEDNVMKIADFGLARGVH t g g t g a c t g a g g a c a a t g t g a t g a a g a t t g c t g a c t t t g g g c t g g c c c g c g g c g t c c a c c a c c a c t g a c t c c t g t t a c a c t a c t t c t a a c g a c t g a a a c c c g a c c g g g c g c c g c a g g t g g

2160 HIDYYKKTSNGRLPVKWMAP a c a t t g a c t a c t a t a a g a a a a c c a g c a a c g g c c g c c t g c c t g t g a a g t g g a t g g c g c c c g t g t a a c t g a t g a t a t t c t t t t g g t c g t t g c c g g c g g a c g g a c a c t t c a c c t a c c g c g g g c

2220 EALFDRVYTHQSDVWSFGIL a g g c c t t g t t t g a c c g g g t g t a c a c a c a c c a g a g t g a c g t g t g g t c t t t t g g g a t c c t g c t c c g g a a c a a a c t g g c c c a c a t g t g t g t g g t c t c a c t g c a c a c c a g a a a a c c c t a g g a c g

2280 LWEIFTLGGSPYPGIPVEEL t a t g g g a g a t c t t c a c c c t c g g g g g c t c c c c g t a t c c t g g c a t c c c g g t g g a g g a g c t g t a t a c c c t c t a g a a g t g g g a g c c c c c g a g g g g c a t a g g a c c g t a g g g c c a c c t c c t c g a c a

2340 FSLLREGHRMDRPPHCPPEL t c t c g c t g c t g c g g g a g g g a c a t c g g a t g g a c c g a c c c c c a c a c t g c c c c c c a g a g c t g t a g a g c g a c g a c g c c c t c c c t g t a g c c t a c c t g g c t g g g g g t g t g a c g g g g g g t c t c g a c a

2400 YGLMRECWHAAPSQRPTFKQ a c g g g c t g a t g c g t g a g t g c t g g c a c g c a g c g c c c t c c c a g a g g c c t a c c t t c a a g c a g c t g c c c g a c t a c g c a c t c a c g a c c g t g c g t c g c g g g a g g g t c t c c g g a t g g a a g t t c g t c g

2460 LVEALDKVLLAVSEEYLDLR t g g t g g a g g c g c t g g a c a a g g t c c t g c t g g c c g t c t c t g a g g a g t a c c t c g a c c t c c g c c a c c a c c t c c g c g a c c t g t t c c a g g a c g a c c g g c a g a g a c t c c t c a t g g a g c t g g a g g c g g

2520 LTFGPYSPSGGDASSTCSSS t g a c c t t c g g a c c c t a t t c c c c c t c t g g t g g g g a c g c c a g c a g c a c c t g c t c c t c c a g c g a c t g g a a g c c t g g g a t a a g g g g g a g a c c a c c c c t g c g g t c g t c g t g g a c g a g g a g g t c g c

2580 DSVFSHDPLPLGSSSFPFGS a t t c t g t c t t c a g c c a c g a c c c c c t g c c a t t g g g a t c c a g c t c c t t c c c c t t c g g g t c t g t a a g a c a g a a g t c g g t g c t g g g g g a c g g t a a c c c t a g g t c g a g g a a g g g g a a g c c c a g a c

2640 attL2 GVQTSTFLYKVGIIGKHCLS g g g t g c a g a c a t c a a c t t t c t t g t a c a a a g t t g g c a t t a t a g g a a a g c a t t g c t t a t c a a c c c a c g t c t g t a g t t g a a a g a a c a t g t t t c a a c c g t a a t a t c c t t t c g t a a c g a a t a g t t

HindIII 2700 attL2 ICCNEQVTISQNKIIICMQA t t t g t t g c a a c g a a c a g g t c a c t a t c a g t c a a a a t a a a a t c a t t a t t t g c a t g c a a g c t t a a a c a a c g t t g c t t g t c c a g t g a t a g t c a g t t t t a t t t t a g t a a t a a a c g t a c g t t c g a a

2760 * a g a t c c t c t a g a c c a g c c a g g a c a g a a a t g c c t c g a c t t c g c t g c t a c c c a a g g t t g c c g t c t a g g a g a t c t g g t c g g t c c t g t c t t t a c g g a g c t g a a g c g a c g a t g g g t t c c a a c g g c

2820 g g t g a c g c a c a c c g t g g a a a c g g a t g a a g g c a c g a a c c c a g t g g a c a t a a g c c t g t t c g g c c a c t g c g t g t g g c a c c t t t g c c t a c t t c c g t g c t t g g g t c a c c t g t a t t c g g a c a a g c c

2880 MRSRNWSRTLT t t c g t a a g c t g t a a t g c a a g t a g c g t a t g c g c t c a c g c a a c t g g t c c a g a a c c t t g a c c g a a g c a t t c g a c a t t a c g t t c a t c g c a t a c g c g a g t g c g t t g a c c a g g t c t t g g a a c t g g c

2940 ERSGGNGAVAVFMACYDCFF a a c g c a g c g g t g g t a a c g g c g c a g t g g c g g t t t t c a t g g c t t g t t a t g a c t g t t t t t t t g t t g c g t c g c c a c c a t t g c c g c g t c a c c g c c a a a a g t a c c g a a c a a t a c t g a c a a a a a a a c

3000 GVQSMPRASKQQARYAVGRC g g g t a c a g t c t a t g c c t c g g g c a t c c a a g c a g c a a g c g c g t t a c g c c g t g g g t c g a t g t t c c c a t g t c a g a t a c g g a g c c c g t a g g t t c g t c g t t c g c g c a a t g c g g c a c c c a g c t a c a a

3060 LMLWSSNDVTQQGSRPKTKL t g a t g t t a t g g a g c a g c a a c g a t g t t a c g c a g c a g g g c a g t c g c c c t a a a a c a a a g t t a a a c t a c a a t a c c t c g t c g t t g c t a c a a t g c g t c g t c c c g t c a g c g g g a t t t t g t t t c a a t t

3120 1 5 10 15 MREAVIAEVSTQLSEVVG SmR NIMREAVIAEVSTQLSEVVG a c a t t a t g a g g g a a g c g g t g a t c g c c g a a g t a t c g a c t c a a c t a t c a g a g g t a g t t g g c g t g t a a t a c t c c c t t c g c c a c t a g c g g c t t c a t a g c t g a g t t g a t a g t c t c c a t c a a c c g c

3180 20 25 30 35 VIERHLEPTLLAVHLYGSAV SmR VIERHLEPTLLAVHLYGSAV t c a t c g a g c g c c a t c t c g a a c c g a c g t t g c t g g c c g t a c a t t t g t a c g g c t c c g c a g t g g a g t a g c t c g c g g t a g a g c t t g g c t g c a a c g a c c g g c a t g t a a a c a t g c c g a g g c g t c a c c

BstEII 3240 40 45 50 55 DGGLKPHSDIDLLVTVTVRL SmR DGGLKPHSDIDLLVTVTVRL a t g g c g g c c t g a a g c c a c a c a g t g a t a t t g a t t t g c t g g t t a c g g t g a c c g t a a g g c t t g t a c c g c c g g a c t t c g g t g t g t c a c t a t a a c t a a a c g a c c a a t g c c a c t g g c a t t c c g a a c

BclI* 3300 60 65 70 75 DETTRRALINDLLETSASPG SmR DETTRRALINDLLETSASPG a t g a a a c a a c g c g g c g a g c t t t g a t c a a c g a c c t t t t g g a a a c t t c g g c t t c c c c t g g a g t a c t t t g t t g c g c c g c t c g a a a c t a g t t g c t g g a a a a c c t t t g a a g c c g a a g g g g a c c t c

3360 80 85 90 95 ESEILRAVEVTIVVHDDIIP SmR ESEILRAVEVTIVVHDDIIP a g a g c g a g a t t c t c c g c g c t g t a g a a g t c a c c a t t g t t g t g c a c g a c g a c a t c a t t c c g t t c t c g c t c t a a g a g g c g c g a c a t c t t c a g t g g t a a c a a c a c g t g c t g c t g t a g t a a g g c a

3420 100 105 110 115 WRYPAKRELQFGEWQRNDIL SmR WRYPAKRELQFGEWQRNDIL g g c g t t a t c c a g c t a a g c g c g a a c t g c a a t t t g g a g a a t g g c a g c g c a a t g a c a t t c t t g c c g c a a t a g g t c g a t t c g c g c t t g a c g t t a a a c c t c t t a c c g t c g c g t t a c t g t a a g a a c

PvuI 3480 120 125 130 135 AGIFEPATIDIDLAILLTKA SmR AGIFEPATIDIDLAILLTKA c a g g t a t c t t c g a g c c a g c c a c g a t c g a c a t t g a t c t g g c t a t c t t g c t g a c a a a a g c a a g t c c a t a g a a g c t c g g t c g g t g c t a g c t g t a a c t a g a c c g a t a g a a c g a c t g t t t t c g t t

3540 140 145 150 155 REHSVALVGPAAEELFDPVP SmR REHSVALVGPAAEELFDPVP g a g a a c a t a g c g t t g c c t t g g t a g g t c c a g c g g c g g a g g a a c t c t t t g a t c c g g t t c c t g c t c t t g t a t c g c a a c g g a a c c a t c c a g g t c g c c g c c t c c t t g a g a a a c t a g g c c a a g g a c

3600 160 165 170 175 EQDLFEALNETLTLWNSPPD SmR EQDLFEALNETLTLWNSPPD a a c a g g a t c t a t t t g a g g c g c t a a a t g a a a c c t t a a c g c t a t g g a a c t c g c c g c c c g a c t t t g t c c t a g a t a a a c t c c g c g a t t t a c t t t g g a a t t g c g a t a c c t t g a g c g g c g g g c t g a

3660 180 185 190 195 WAGDERNVVLTLSRIWYSAV SmR WAGDERNVVLTLSRIWYSAV g g g c t g g c g a t g a g c g a a a t g t a g t g c t t a c g t t g t c c c g c a t t t g g t a c a g c g c a g t a a c c c g a c c g c t a c t c g c t t t a c a t c a c g a a t g c a a c a g g g c g t a a a c c a t g t c g c g t c a t t

3720 200 205 210 215 TGKIAPKDVAADWAMERLPA SmR TGKIAPKDVAADWAMERLPA c c g g c a a a a t c g c g c c g a a g g a t g t c g c t g c c g a c t g g g c a a t g g a g c g c c t g c c g g c c c g g c c g t t t t a g c g c g g c t t c c t a c a g c g a c g g c t g a c c c g t t a c c t c g c g g a c g g c c g g g

3780 220 225 230 235 QYQPVILEARQAYLGQEEDR SmR QYQPVILEARQAYLGQEEDR a g t a t c a g c c c g t c a t a c t t g a a g c t a g a c a g g c t t a t c t t g g a c a a g a a g a a g a t c g c t t c a t a g t c g g g c a g t a t g a a c t t c g a t c t g t c c g a a t a g a a c c t g t t c t t c t t c t a g c g a

BssHII 3840 240 245 250 255 LASRADQLEEFVHYVKGEIT SmR LASRADQLEEFVHYVKGEIT t g g c c t c g c g c g c a g a t c a g t t g g a a g a a t t t g t c c a c t a c g t g a a a g g c g a g a t c a c c a a c c g g a g c g c g c g t c t a g t c a a c c t t c t t a a a c a g g t g a t g c a c t t t c c g c t c t a g t g g t

PaeR7IPspXIXhoI 3900 260 KVVGK* SmR KVVGK* a g g t a g t c g g c a a a t a a c c c t c g a g c c a c c c a t g a c c a a a a t c c c t t a a c g t g a g t t a c g t c c a t c a g c c g t t t a t t g g g a g c t c g g t g g g t a c t g g t t t t a g g g a a t t g c a c t c a a t g c

3960 ori c g t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t g c a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a

4020 ori t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a

4080 ori g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g

4140 ori a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c

4200 ori t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c

4260 ori a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a

4320 ori c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g

4380 ori t g a g a t a c c t a c a g c g t g a g c a t t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c t c t a t g g a t g t c g c a c t c g t a a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c

4440 ori G G pBR322ori-F a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c

DrdI 4500 ori G A A A C G C C T G G T A T C T T T pBR322ori-F g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a

4560 ori t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a

PciI 4620 t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c

4680 a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t

4740 A G C G A G T C A G T G A G C G A G L4440 c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g

4800 c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t

4860 a a g c g g g c a g t g a g c g c a a c g c a a t t a a t a c g c g t a c c g c t a g c c a g g a a g a g t t t g t a g t t c g c c c g t c a c t c g c g t t g c g t t a a t t a t g c g c a t g g c g a t c g g t c c t t c t c a a a c a t c

4920 rrnB T2 terminator a a a c g c a a a a a g g c c a t c c g t c a g g a t g g c c t t c t g c t t a g t t t g a t g c c t g g c a g t t t a t t t g c g t t t t t c c g g t a g g c a g t c c t a c c g g a a g a c g a a t c a a a c t a c g g a c c g t c a a a t

AclI 4980 t g g c g g g c g t c c t g c c c g c c a c c c t c c g g g c c g t t g c t t c a c a a c g t t c a a a t c c g c t c c a c c g c c c g c a g g a c g g g c g g t g g g a g g c c c g g c a a c g a a g t g t t g c a a g t t t a g g c g a g g

5040 rrnB T1 terminator c g g c g g a t t t g t c c t a c t c a g g a g a g c g t t c a c c g a c a a a c a a c a g a t a a a a c g a a a g g c g c c g c c t a a a c a g g a t g a g t c c t c t c g c a a g t g g c t g t t t g t t g t c t a t t t t g c t t t c c g

5099 rrnB T1 terminator c c a g t c t t c c g a c t g a g c c t t t c g t t t t a t t t g a t g c c t g g c a g t t c c c t a c t c t c g c g g g t c a g a a g g c t g a c t c g g a a a g c a a a a t a a a c t a c g g a c c g t c a a g g g a t g a g a g c g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.