Addgene pMLLCK-J72029 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pMLLCK-J72029


Map View

EcoRI (2691) BssSI - BssSαI (2634) ApaI (2554) PspOMI (2550) PsiI (2391) SnaBI (2182) BsiHKAI - SacI (2042) Eco53kI (2040) EcoRV (2034) ScaI (1999) MscI (1849) EaeI (1847) PasI (1814) AclI (1669) BspEI (1578) PvuII (1482) CAT-R (1435 .. 1454) BglII (0) BamHI (54) Bsu36I (70) L4440 (80 .. 97) BsrBI (123) BseYI (160) PspFI (164) EcoP15I (165) DraIII (244) pENTR-R (333 .. 352) NruI (432) Kan-R (417 .. 436) BspDI - ClaI (466) EarI (588) TspMI - XmaI (647) SmaI (649) EcoNI (687) BsrFI (729) AsiSI - PvuI (775) BbsI - PaeR7I - SmlI - XhoI (1231) SfcI (1237) PstI (1241) MslI (1298) pMLLCK-J72029 2700 bp

Sequence View

BamHIBglII 60 1 5 10 15 GLNDIFEAQKIEWHE * AviTag™ g a t c t g g c c t g a a c g a t a t t t t t g a a g c g c a g a a a a t t g a a t g g c a t g a a t a a g g a t c c t c t a g a c c g g a c t t g c t a t a a a a a c t t c g c g t c t t t t a a c t t a c c g t a c t t a t t c c t a g g a

Bsu36I 120 G A G C G A G T G A C T G A G C G A L4440 a a c t c g c t c c t c a g g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c t t g a g c g a g g a g t c c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g

BseYIPspFIEcoP15IBsrBI 180 attB2 g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t c a a t t c g a c c c a g c t t t c t t g t a c a a a g t c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a g t t a a g c t g g g t c g a a a g a a c a t g t t t c a

240 attB2 g g t t g a t c g g a t c t g c t g a t c c t t c a a c t c a g c a a a a g t t c g a t t t a t t c a a c a a a g c c a c c a a c t a g c c t a g a c g a c t a g g a a g t t g a g t c g t t t t c a a g c t a a a t a a g t t g t t t c g g t

DraIII 300 c g t t g t g t c t c a a a a t c t c t g a t g t t a c a t t g c a c a a g a t a a a a a t a t a t c a t c a t g a a c g c a a c a c a g a g t t t t a g a g a c t a c a a t g t a a c g t g t t c t a t t t t t a t a t a g t a g t a c t t g

360 1 5 MSHIQR KanR 1 5 MSHIQR KanR G T T C C C C A C A A T A C T C G G T A pENTR-R MSHIQR a a t a a a a c t g t c t g c t t a c a t a a a c a g t a a t a c a a g g g g t g t t a t g a g c c a t a t t c a a c g t t a t t t t g a c a g a c g a a t g t a t t t g t c a t t a t g t t c c c c a c a a t a c t c g g t a t a a g t t g c

420 10 15 20 25 ETSCSRPRLNSNMDADLYGY KanR 10 15 20 25 ETSCSRPRLNSNMDADLYGY KanR C C A T Kan-R ETSCSRPRLNSNMDADLYGY g g a a a c g t c t t g c t c c a g g c c g c g a t t a a a t t c c a a c a t g g a t g c t g a t t t a t a t g g g t a c c t t t g c a g a a c g a g g t c c g g c g c t a a t t t a a g g t t g t a c c t a c g a c t a a a t a t a c c c a t

BspDIClaINruI 480 30 35 40 45 KWARDNVGQSGATIYRLYGK KanR 30 35 40 45 KWARDNVGQSGATIYRLYGK KanR A T T T A C C C G A G C G C T A Kan-R KWARDNVGQSGATIYRLYGK t a a a t g g g c t c g c g a t a a t g t c g g g c a a t c a g g t g c g a c a a t c t a t c g a t t g t a t g g g a a a t t t a c c c g a g c g c t a t t a c a g c c c g t t a g t c c a c g c t g t t a g a t a g c t a a c a t a c c c t t

540 50 55 60 65 PDAPELFLKHGKGSVANDVT KanR 50 55 60 65 PDAPELFLKHGKGSVANDVT KanR PDAPELFLKHGKGSVANDVT g c c c g a t g c g c c a g a g t t g t t t c t g a a a c a t g g c a a a g g t a g c g t t g c c a a t g a t g t t a c c g g g c t a c g c g g t c t c a a c a a a g a c t t t g t a c c g t t t c c a t c g c a a c g g t t a c t a c a a t g

EarI 600 70 75 80 85 DEMVRLNWLTEFMPLPTIKH KanR 70 75 80 85 DEMVRLNWLTEFMPLPTIKH KanR DEMVRLNWLTEFMPLPTIKH a g a t g a g a t g g t c a g a c t a a a c t g g c t g a c g g a a t t t a t g c c t c t t c c g a c c a t c a a g c a t c t a c t c t a c c a g t c t g a t t t g a c c g a c t g c c t t a a a t a c g g a g a a g g c t g g t a g t t c g t

TspMIXmaISmaI 660 90 95 100 105 FIRTPDDAWLLTTAIPGKTA KanR 90 95 100 105 FIRTPDDAWLLTTAIPGKTA KanR FIRTPDDAWLLTTAIPGKTA t t t t a t c c g t a c t c c t g a t g a t g c a t g g t t a c t c a c c a c t g c g a t c c c c g g g a a a a c a g c a a a a t a g g c a t g a g g a c t a c t a c g t a c c a a t g a g t g g t g a c g c t a g g g g c c c t t t t g t c g

EcoNI 720 110 115 120 125 FQVLEEYPDSGENIVDALAV KanR 110 115 120 125 FQVLEEYPDSGENIVDALAV KanR FQVLEEYPDSGENIVDALAV a t t c c a g g t a t t a g a a g a a t a t c c t g a t t c a g g t g a a a a t a t t g t t g a t g c g c t g g c a g t t a a g g t c c a t a a t c t t c t t a t a g g a c t a a g t c c a c t t t t a t a a c a a c t a c g c g a c c g t c a

AsiSIPvuIBsrFI 780 130 135 140 145 FLRRLHSIPVCNCPFNSDRV KanR 130 135 140 145 FLRRLHSIPVCNCPFNSDRV KanR FLRRLHSIPVCNCPFNSDRV g t t c c t g c g c c g g t t g c a t t c g a t t c c t g t t t g t a a t t g t c c t t t t a a c a g c g a t c g c g t c a a g g a c g c g g c c a a c g t a a g c t a a g g a c a a a c a t t a a c a g g a a a a t t g t c g c t a g c g c a

840 150 155 160 165 FRLAQAQSRMNNGLVDASDF KanR 150 155 160 165 FRLAQAQSRMNNGLVDASDF KanR FRLAQAQSRMNNGLVDASDF a t t t c g t c t c g c t c a g g c g c a a t c a c g a a t g a a t a a c g g t t t g g t t g a t g c g a g t g a t t t t a a a g c a g a g c g a g t c c g c g t t a g t g c t t a c t t a t t g c c a a a c c a a c t a c g c t c a c t a a a

900 170 175 180 185 DDERNGWPVEQVWKEMHKLL KanR 170 175 180 185 DDERNGWPVEQVWKEMHKLL KanR DDERNGWPVEQVWKEMHKLL t g a t g a c g a g c g t a a t g g c t g g c c t g t t g a a c a a g t c t g g a a a g a a a t g c a t a a g c t t t t a c t a c t g c t c g c a t t a c c g a c c g g a c a a c t t g t t c a g a c c t t t c t t t a c g t a t t c g a a a a

960 190 195 200 205 PFSPDSVVTHGDFSLDNLIF KanR 190 195 200 205 PFSPDSVVTHGDFSLDNLIF KanR PFSPDSVVTHGDFSLDNLIF g c c a t t c t c a c c g g a t t c a g t c g t c a c t c a t g g t g a t t t c t c a c t t g a t a a c c t t a t t t t c g g t a a g a g t g g c c t a a g t c a g c a g t g a g t a c c a c t a a a g a g t g a a c t a t t g g a a t a a a a

1020 210 215 220 225 DEGKLIGCIDVGRVGIADRY KanR 210 215 220 225 DEGKLIGCIDVGRVGIADRY KanR DEGKLIGCIDVGRVGIADRY t g a c g a g g g g a a a t t a a t a g g t t g t a t t g a t g t t g g a c g a g t c g g a a t c g c a g a c c g a t a a c t g c t c c c c t t t a a t t a t c c a a c a t a a c t a c a a c c t g c t c a g c c t t a g c g t c t g g c t a t

1080 230 235 240 245 QDLAILWNCLGEFSPSLQKR KanR 230 235 240 245 QDLAILWNCLGEFSPSLQKR KanR QDLAILWNCLGEFSPSLQKR c c a g g a t c t t g c c a t c c t a t g g a a c t g c c t c g g t g a g t t t t c t c c t t c a t t a c a g a a a c g g g t c c t a g a a c g g t a g g a t a c c t t g a c g g a g c c a c t c a a a a g a g g a a g t a a t g t c t t t g c

1140 250 255 260 265 LFQKYGIDNPDMNKLQFHLM KanR 250 255 260 265 LFQKYGIDNPDMNKLQFHLM KanR LFQKYGIDNPDMNKLQFHLM g c t t t t t c a a a a a t a t g g t a t t g a t a a t c c t g a t a t g a a t a a a t t g c a g t t t c a t t t g a t c g a a a a a g t t t t t a t a c c a t a a c t a t t a g g a c t a t a c t t a t t t a a c g t c a a a g t a a a c t a

1200 270 LDEFF* KanR 270 LDEFF* KanR LDEFF* g c t c g a t g a g t t t t t c t a a t c a g a a t t g g t t a a t t g g t t g t a a c a c t g g c a g a g c a t t a c c g a g c t a c t c a a a a a g a t t a g t c t t a a c c a a t t a a c c a a c a t t g t g a c c g t c t c g t a a t g

PstISfcIPaeR7IXhoISmlIBbsI 1260 g c t g a c t t g a c g g g a g g a t c t c t g a a g a c t c t c g a g c t g c a g g a c t c a c a g c t c c t c g g a c g a c t g a a c t g c c c t c c t a g a g a c t t c t g a g a g c t c g a c g t c c t g a g t g t c g a g g a g c c t

MslI 1320 t c t t t g a t c g g g c a c g t a a g a g g t t c c a a c t t t c a c c a t a a t g a a a t a a g a t c a c t a c c g a g a a a c t a g c c c g t g c a t t c t c c a a g g t t g a a a g t g g t a t t a c t t t a t t c t a g t g a t g g c

1380 1 MEKK CmR MEKK g g c g t a t t t t t t g a g t t a t c g a g a t t t t c a g g a g c t a a g g a a g c t a a a a t g g a g a a a a a a c c g c a t a a a a a a c t c a a t a g c t c t a a a a g t c c t c g a t t c c t t c g a t t t t a c c t c t t t t t t

1440 5 10 15 20 ITGYTTVDISQWHRKEHFEA CmR C T C C G T CAT-R ITGYTTVDISQWHRKEHFEA a t c a c t g g a t a t a c c a c c g t t g a t a t a t c c c a a t g g c a t c g t a a a g a a c a t t t t g a g g c a t a g t g a c c t a t a t g g t g g c a a c t a t a t a g g g t t a c c g t a g c a t t t c t t g t a a a a c t c c g t

PvuII 1500 25 30 35 40 FQSVAQCTYNQTVQLDITAF CmR A A A G T C A G T C A A C G CAT-R FQSVAQCTYNQTVQLDITAF t t t c a g t c a g t t g c t c a a t g t a c c t a t a a c c a g a c c g t t c a g c t g g a t a t t a c g g c c t t t a a a g t c a g t c a a c g a g t t a c a t g g a t a t t g g t c t g g c a a g t c g a c c t a t a a t g c c g g a a a

1560 45 50 55 60 LKTVKKNKHKFYPAFIHILA CmR LKTVKKNKHKFYPAFIHILA t t a a a g a c c g t a a a g a a a a a t a a g c a c a a g t t t t a t c c g g c c t t t a t t c a c a t t c t t g c c a a t t t c t g g c a t t t c t t t t t a t t c g t g t t c a a a a t a g g c c g g a a a t a a g t g t a a g a a c g g

BspEI 1620 65 70 75 80 RLMNAHPEFRMAMKDGELVI CmR RLMNAHPEFRMAMKDGELVI c g c c t g a t g a a t g c t c a t c c g g a a t t t c g t a t g g c a a t g a a a g a c g g t g a g c t g g t g a t a g c g g a c t a c t t a c g a g t a g g c c t t a a a g c a t a c c g t t a c t t t c t g c c a c t c g a c c a c t a t

AclI 1680 85 90 95 100 WDSVHPCYTVFHEQTETFSS CmR WDSVHPCYTVFHEQTETFSS t g g g a t a g t g t t c a c c c t t g t t a c a c c g t t t t c c a t g a g c a a a c t g a a a c g t t t t c a t c g a c c c t a t c a c a a g t g g g a a c a a t g t g g c a a a a g g t a c t c g t t t g a c t t t g c a a a a g t a g c

1740 105 110 115 120 LWSEYHDDFRQFLHIYSQDV CmR LWSEYHDDFRQFLHIYSQDV c t c t g g a g t g a a t a c c a c g a c g a t t t c c g g c a g t t t c t a c a c a t a t a t t c g c a a g a t g t g g a g a c c t c a c t t a t g g t g c t g c t a a a g g c c g t c a a a g a t g t g t a t a t a a g c g t t c t a c a c

1800 125 130 135 140 ACYGENLAYFPKGFIENMFF CmR ACYGENLAYFPKGFIENMFF g c g t g t t a c g g t g a a a a c c t g g c c t a t t t c c c t a a a g g g t t t a t t g a g a a t a t g t t t t t c c g c a c a a t g c c a c t t t t g g a c c g g a t a a a g g g a t t t c c c a a a t a a c t c t t a t a c a a a a a g

EaeIMscIPasI 1860 145 150 155 160 VSANPWVSFTSFDLNVANMD CmR VSANPWVSFTSFDLNVANMD g t c t c a g c c a a t c c c t g g g t g a g t t t c a c c a g t t t t g a t t t a a a c g t g g c c a a t a t g g a c c a g a g t c g g t t a g g g a c c c a c t c a a a g t g g t c a a a a c t a a a t t t g c a c c g g t t a t a c c t g

1920 165 170 175 180 NFFAPVFTMGKYYTQGDKVL CmR NFFAPVFTMGKYYTQGDKVL a a c t t c t t c g c c c c c g t t t t c a c c a t g g g c a a a t a t t a t a c g c a a g g c g a c a a g g t g c t g t t g a a g a a g c g g g g g c a a a a g t g g t a c c c g t t t a t a a t a t g c g t t c c g c t g t t c c a c g a c

1980 185 190 195 200 MPLAIQVHHAVCDGFHVGRM CmR MPLAIQVHHAVCDGFHVGRM a t g c c g c t g g c g a t t c a g g t t c a t c a t g c c g t t t g t g a t g g c t t c c a t g t c g g c a g a a t g t a c g g c g a c c g c t a a g t c c a a g t a g t a c g g c a a a c a c t a c c g a a g g t a c a g c c g t c t t a c

Eco53kIEcoRVScaI 2040 205 210 215 220 LNELQQYCDEWQGGA* CmR LNELQQYCDEWQGGA* c t t a a t g a a t t a c a a c a g t a c t g c g a t g a g t g g c a g g g c g g g g c g t a a t t t g a t a t c g a g g a a t t a c t t a a t g t t g t c a t g a c g c t a c t c a c c g t c c c g c c c c g c a t t a a a c t a t a g c t c

BsiHKAISacI 2100 lambda t0 terminator c t c g c t t g g a c t c c t g t t g a t a g a t c c a g t a a t g a c c t c a g a a c t c c a t c t g g a t t t g t t g a g c g a a c c t g a g g a c a a c t a t c t a g g t c a t t a c t g g a g t c t t g a g g t a g a c c t a a a c a a

2160 lambda t0 terminator c a g a a c g c t c g g t t g c c g c c g g g c g t t t t t t a t t g g t g a g a a t c c a a g c c t c g g a t c t a g g t c t t g c g a g c c a a c g g c g g c c c g c a a a a a a t a a c c a c t c t t a g g t t c g g a g c c t a g a t c

SnaBI 2220 R6K γ ori c a g t t c a a c c t g t t g a t a g t a c g t a c t a a g c t c t c a t g t t t c a c g t a c t a a g c t c t c a t g g t c a a g t t g g a c a a c t a t c a t g c a t g a t t c g a g a g t a c a a a g t g c a t g a t t c g a g a g t a c

2280 R6K γ ori t t t a a c g t a c t a a g c t c t c a t g t t t a a c g a a c t a a a c c c t c a t g g c t a a c g t a c t a a g c t a a a t t g c a t g a t t c g a g a g t a c a a a t t g c t t g a t t t g g g a g t a c c g a t t g c a t g a t t c g a

2340 R6K γ ori c t c a t g g c t a a c g t a c t a a g c t c t c a t g t t t c a c g t a c t a a g c t c t c a t g t t t g a a c a a t g a g t a c c g a t t g c a t g a t t c g a g a g t a c a a a g t g c a t g a t t c g a g a g t a c a a a c t t g t t a

PsiI 2400 R6K γ ori a a a a t t a a t a t a a a t c a g c a a c t t a a a t a g c c t c t a a g g t t t t a a g t t t t a t a a g a a a a a t t t t a a t t a t a t t t a g t c g t t g a a t t t a t c g g a g a t t c c a a a a t t c a a a a t a t t c t t t t t

2460 R6K γ ori a a a g a a t a t a t a a g g c t t t t a a a g c t t t t a a g g t t t a a c g g t t g t g g a c a a c a a g c c a g g t t t c t t a t a t a t t c c g a a a a t t t c g a a a a t t c c a a a t t g c c a a c a c c t g t t g t t c g g t c c

2520 R6K γ ori g a t g t a a c g c a c t g a g a a g c c c t t a g a g c c t c t c a a a g c a a t t t t g a g t g a c a c a g g a a c c t a c a t t g c g t g a c t c t t c g g g a a t c t c g g a g a g t t t c g t t a a a a c t c a c t g t g t c c t t g

PspOMIApaI 2580 R6K γ ori a c t t a a c g g c t g a c a t g g g a a t t a g c c a t g g g c c c g t g c g a a t c a c g g a t c t c c c g a t c a t g a a t t g c c g a c t g t a c c c t t a a t c g g t a c c c g g g c a c g c t t a g t g c c t a g a g g g c t a g t

BssSαIBssSI 2640 attB1 a g a a t t g c c a t t a t c a a c a a g t t t g t a c a a a a a a g c a g g c t c c g a a t t g g t a t c a c g a g g t c t t a a c g g t a a t a g t t g t t c a a a c a t g t t t t t t c g t c c g a g g c t t a a c c a t a g t g c t c c

EcoRI 2700 c a g a a t t t c a g a t a a a a a a a a t c c t t a g c t t t c g c t a a g g a t c t g a a g t g g a a t t c a t g a g t c t t a a a g t c t a t t t t t t t t a g g a a t c g a a a g c g a t t c c t a g a c t t c a c c t t a a g t a c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.