Addgene 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

Map View

pGEX 3' (29 .. 51) pBRforEco (8335 .. 8353) AatII (8297) ZraI (8295) AmpR promoter SspI (8179) Amp-R (7925 .. 7944) pBR322ori-F (6623 .. 6642) PciI (6482) L4440 (6372 .. 6389) BspQI - SapI (6366) CAP binding site lac promoter M13/pUC Reverse (6154 .. 6176) lac operator M13 Reverse (6141 .. 6157) M13 rev SbfI (6115) XcmI (5669) BbsI (5573) bGH poly(A) signal BGH-rev (5378 .. 5395) BsaBI * (5357) EagI - NotI (5339) FseI (5318) NaeI (5316) NgoMIV (5314) MluI (5295) SV40 poly(A) signal SV40pA-R (5210 .. 5229) NsiI (5074) CsiI - SexAI * (4630) Bsu36I (4542) BstEII (4451) BmtI (3769) pRS-marker (151 .. 170) BstAPI (185) KasI (235) NarI (236) SfoI (237) PluTI (239) M13/pUC Forward (364 .. 386) M13 Forward (378 .. 395) M13 fwd EcoRI (396) BglII (402) EcoRV (410) PshAI (576) NruI (694) U6 promoter gRNA scaffold AgeI (1049) U6 promoter gRNA scaffold BspDI - ClaI (1407) U6 promoter gRNA scaffold Acc65I (1763) KpnI (1767) U6 promoter gRNA scaffold Eco53kI (2122) PspXI (2123) SacI (2124) U6 promoter gRNA scaffold SpeI (2483) PmeI (2654) PacI (2960) NcoI (2974) EGFP-N (3021 .. 3042) EXFP-R (3282 .. 3301) PflFI - Tth111I (3423) BlpI (3597) EGFP-C (3629 .. 3650) P2A BmgBI (3743) NheI (3765) 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO 8362 bp

Sequence View

5ʹ 3ʹ 60 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' t c g c g c g t t t c g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a a g c g c g c a a a g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t

120 c a g c t t g t c t g t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g g t c g a a c a g a c a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c

180 C G G C A T C A G A G C A G A T T G T A pRS-marker t t g g c g g g t g t c g g g g c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a a c c g c c c a c a g c c c c g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g

KasINarISfoIPluTIBstAPI 240 a c c a t a t g c g g t g t g a a a t a c c g c a c a g a t g c g t a a g g a g a a a a t a c c g c a t c a g g c g c c t g g t a t a c g c c a c a c t t t a t g g c g t g t c t a c g c a t t c c t c t t t t a t g g c g t a g t c c g c g g

300 a t t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a

360 t a c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c c a g g g t a t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g g t c c c a

EcoRVBglIIEcoRI 420 M13 fwd C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t g a a t t c a g a t c t g a t a t c t c t a g a g a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a c t t a a g t c t a g a c t a t a g a g a t c t c

480 a a g t c g a c g t t t a a a g t a c c c t a c a g t t g a a g t c g g a a g t t t a c a t a c a c t t a a g t t g g a t t c a g c t g c a a a t t t c a t g g g a t g t c a a c t t c a g c c t t c a a a t g t a t g t g a a t t c a a c c t

540 g t c a t t a a a a c t c g t t t t t c a a c t a c t c c a c a a a t t t c t t g t t a a c a a a c a a t a g t t t t g c a g t a a t t t t g a g c a a a a a g t t g a t g a g g t g t t t a a a g a a c a a t t g t t t g t t a t c a a a a c

PshAI 600 g c a a g t c a g t t a g g a c a t c t a c t t t g t g c a t g a c a c a a g t c a t t t t t c c a a c a a t t g t t t c g t t c a g t c a a t c c t g t a g a t g a a a c a c g t a c t g t g t t c a g t a a a a a g g t t g t t a a c a a a

660 a c a g a c a g a t t a t t t c a c t t a t a a t t c a c t g t a t c a c a a t t c c a g t g g g t c a g a a g t t t a t g t c t g t c t a a t a a a g t g a a t a t t a a g t g a c a t a g t g t t a a g g t c a c c c a g t c t t c a a a t

NruI 720 U6 promoter c a t a c a c t a a g t t g a c t g t g c c t t t a a a c a g t c g c g a g a g g g c c t a t t t c c c a t g a t t c c g t a t g t g a t t c a a c t g a c a c g g a a a t t t g t c a g c g c t c t c c c g g a t a a a g g g t a c t a a g g

780 U6 promoter t t c a t a t t t g c a t a t a c g a t a c a a g g c t g t t a g a g a g a t a a t t g g a a t t a a t t t g a c t g t a a g t a t a a a c g t a t a t g c t a t g t t c c g a c a a t c t c t c t a t t a a c c t t a a t t a a a c t g a c a

840 U6 promoter a a a c a c a a a g a t a t t a g t a c a a a a t a c g t g a c g t a g a a a g t a a t a a t t t c t t g g g t a g t t t t t g t g t t t c t a t a a t c a t g t t t t a t g c a c t g c a t c t t t c a t t a t t a a a g a a c c c a t c a a

900 U6 promoter t g c a g t t t t a a a a t t a t g t t t t a a a a t g g a c t a t c a t a t g c t t a c c g t a a c t t g a a a g t a a c g t c a a a a t t t t a a t a c a a a a t t t t a c c t g a t a g t a t a c g a a t g g c a t t g a a c t t t c a t

960 U6 promoter t t t c g a t t t c t t g g c t t t a t a t a t c t t g t g g a a a g g a c g a a a c a c c g c a g c a a t t c a c t g a a a g c t a a a g a a c c g a a a t a t a t a g a a c a c c t t t c c t g c t t t g t g g c g t c g t t a a g t g a c

1020 gRNA scaffold t a a a g c g t t t t a g a g c t a g a a a t a g c a a g t t a a a a t a a g g c t a g t c c g t t a t c a a c t t g a a t t t c g c a a a a t c t c g a t c t t t a t c g t t c a a t t t t a t t c c g a t c a g g c a a t a g t t g a a c t

AgeI 1080 gRNA scaffold U6 promoter a a a a g t g g c a c c g a g t c g g t g c t t t t t t a c c g g t g a g g g c c t a t t t c c c a t g a t t c c t t c t t t t c a c c g t g g c t c a g c c a c g a a a a a a t g g c c a c t c c c g g a t a a a g g g t a c t a a g g a a g

1140 U6 promoter a t a t t t g c a t a t a c g a t a c a a g g c t g t t a g a g a g a t a a t t g g a a t t a a t t t g a c t g t a a a t a t a a a c g t a t a t g c t a t g t t c c g a c a a t c t c t c t a t t a a c c t t a a t t a a a c t g a c a t t t

1200 U6 promoter c a c a a a g a t a t t a g t a c a a a a t a c g t g a c g t a g a a a g t a a t a a t t t c t t g g g t a g t t t g c g t g t t t c t a t a a t c a t g t t t t a t g c a c t g c a t c t t t c a t t a t t a a a g a a c c c a t c a a a c g

1260 U6 promoter a g t t t t a a a a t t a t g t t t t a a a a t g g a c t a t c a t a t g c t t a c c g t a a c t t g a a a g t a t t t t c a a a a t t t t a a t a c a a a a t t t t a c c t g a t a g t a t a c g a a t g g c a t t g a a c t t t c a t a a a

1320 U6 promoter c g a t t t c t t g g c t t t a t a t a t c t t g t g g a a a g g a c g a a a c a c c g t g c g t g t t t g t g c c t g g c t a a a g a a c c g a a a t a t a t a g a a c a c c t t t c c t g c t t t g t g g c a c g c a c a a a c a c g g a c

1380 gRNA scaffold t c c g t t t t a g a g c t a g a a a t a g c a a g t t a a a a t a a g g c t a g t c c g t t a t c a a c t t g a a a a a g g c a a a a t c t c g a t c t t t a t c g t t c a a t t t t a t t c c g a t c a g g c a a t a g t t g a a c t t t t

BspDIClaI 1440 gRNA scaffold U6 promoter a g t g g c a c c g a g t c g g t g c t t t t t t a t c g a t g a g g g c c t a t t t c c c a t g a t t c c t t c a t a t c a c c g t g g c t c a g c c a c g a a a a a a t a g c t a c t c c c g g a t a a a g g g t a c t a a g g a a g t a t

1500 U6 promoter t t t g c a t a t a c g a t a c a a g g c t g t t a g a g a g a t a a t t g g a a t t a a t t t g a c t g t a a a c a c a a a c g t a t a t g c t a t g t t c c g a c a a t c t c t c t a t t a a c c t t a a t t a a a c t g a c a t t t g t g

1560 U6 promoter a a a g a t a t t a g t a c a a a a t a c g t g a c g t a g a a a g t a a t a a t t t c t t g g g t a g t t t g c a g t t t t c t a t a a t c a t g t t t t a t g c a c t g c a t c t t t c a t t a t t a a a g a a c c c a t c a a a c g t c a

1620 U6 promoter t t t a a a a t t a t g t t t t a a a a t g g a c t a t c a t a t g c t t a c c g t a a c t t g a a a g t a t t t c g a a a a t t t t a a t a c a a a a t t t t a c c t g a t a g t a t a c g a a t g g c a t t g a a c t t t c a t a a a g c t

1680 U6 promoter t t t c t t g g c t t t a t a t a t c t t g t g g a a a g g a c g a a a c a c c g a a g g a g a a a a a a g a t g a a t a a a g a a c c g a a a t a t a t a g a a c a c c t t t c c t g c t t t g t g g c t t c c t c t t t t t t c t a c t t a

1740 gRNA scaffold g t t t t a g a g c t a g a a a t a g c a a g t t a a a a t a a g g c t a g t c c g t t a t c a a c t t g a a a a a g t c a a a a t c t c g a t c t t t a t c g t t c a a t t t t a t t c c g a t c a g g c a a t a g t t g a a c t t t t t c a

Acc65IKpnI 1800 gRNA scaffold U6 promoter g g c a c c g a g t c g g t g c t t t t t t g g t a c c g a g g g c c t a t t t c c c a t g a t t c c t t c a t a t t t c c g t g g c t c a g c c a c g a a a a a a c c a t g g c t c c c g g a t a a a g g g t a c t a a g g a a g t a t a a a

1860 U6 promoter g c a t a t a c g a t a c a a g g c t g t t a g a g a g a t a a t t g g a a t t a a t t t g a c t g t a a a c a c a a a c g t a t a t g c t a t g t t c c g a c a a t c t c t c t a t t a a c c t t a a t t a a a c t g a c a t t t g t g t t t

1920 U6 promoter g a t a t t a g t a c a a a a t a c g t g a c g t a g a a a g t a a t a a t t t c t t g g g t a g t t t g c a g t t t t c t a t a a t c a t g t t t t a t g c a c t g c a t c t t t c a t t a t t a a a g a a c c c a t c a a a c g t c a a a a

1980 U6 promoter a a a a t t a t g t t t t a a a a t g g a c t a t c a t a t g c t t a c c g t a a c t t g a a a g t a t t t c g a t t t t t t t a a t a c a a a a t t t t a c c t g a t a g t a t a c g a a t g g c a t t g a a c t t t c a t a a a g c t a a a

2040 U6 promoter gRNA scaffold c t t g g c t t t a t a t a t c t t g t g g a a a g g a c g a a a c a c c g t g c a a c a g c t c c t g c a t g g g t t g a a c c g a a a t a t a t a g a a c a c c t t t c c t g c t t t g t g g c a c g t t g t c g a g g a c g t a c c c a a

2100 gRNA scaffold t t a g a g c t a g a a a t a g c a a g t t a a a a t a a g g c t a g t c c g t t a t c a a c t t g a a a a a g t g g c a a t c t c g a t c t t t a t c g t t c a a t t t t a t t c c g a t c a g g c a a t a g t t g a a c t t t t t c a c c g

Eco53kIPspXISacI 2160 gRNA scaffold U6 promoter a c c g a g t c g g t g c t t t t t t g a g c t c g a g g g c c t a t t t c c c a t g a t t c c t t c a t a t t t g c a t g g c t c a g c c a c g a a a a a a c t c g a g c t c c c g g a t a a a g g g t a c t a a g g a a g t a t a a a c g t

2220 U6 promoter t a t a c g a t a c a a g g c t g t t a g a g a g a t a a t t g g a a t t a a t t t g a c t g t a a a c a c a a a g a t a t a t g c t a t g t t c c g a c a a t c t c t c t a t t a a c c t t a a t t a a a c t g a c a t t t g t g t t t c t a

2280 U6 promoter a t t a g t a c a a a a t a c g t g a c g t a g a a a g t a a t a a t t t c t t g g g t a g t t t g c a g t t t t a a a t a a t c a t g t t t t a t g c a c t g c a t c t t t c a t t a t t a a a g a a c c c a t c a a a c g t c a a a a t t t

2340 U6 promoter a t t a t g t t t t a a a a t g g a c t a t c a t a t g c t t a c c g t a a c t t g a a a g t a t t t c g a t t t c t t t a a t a c a a a a t t t t a c c t g a t a g t a t a c g a a t g g c a t t g a a c t t t c a t a a a g c t a a a g a a

2400 U6 promoter gRNA scaffold g g c t t t a t a t a t c t t g t g g a a a g g a c g a a a c a c c g t c a a c t c t c c a a t g t c c a c g t t t t a c c g a a a t a t a t a g a a c a c c t t t c c t g c t t t g t g g c a g t t g a g a g g t t a c a g g t g c a a a a t

2460 gRNA scaffold g a g c t a g a a a t a g c a a g t t a a a a t a a g g c t a g t c c g t t a t c a a c t t g a a a a a g t g g c a c c c t c g a t c t t t a t c g t t c a a t t t t a t t c c g a t c a g g c a a t a g t t g a a c t t t t t c a c c g t g g

SpeI 2520 gRNA scaffold g a g t c g g t g c t t t t t t c t c g a g a c t a g t t a t g a t a g c a t c t t g t t c t t a g t c t t t t t c t t c t c a g c c a c g a a a a a a g a g c t c t g a t c a a t a c t a t c g t a g a a c a a g a a t c a g a a a a a g a a

2580 a a t a g g g a c a t a a a g c c c a c a a a t a a a a a t a t g c c t g a a g a a t g g g a c a g g c a t t g g g c a t t a t c c c t g t a t t t c g g g t g t t t a t t t t t a t a c g g a c t t c t t a c c c t g t c c g t a a c c c g t

2640 t t g t c c a t g c c t a g t a a a g t a c t c c a a g a a c c t a t t t g t a t a c t a g a t g a c a c a a t g t a a a a c a g g t a c g g a t c a t t t c a t g a g g t t c t t g g a t a a a c a t a t g a t c t a c t g t g t t a c a t t

PmeI 2700 t a g t c a t c a t g t t t a a a c a t c t a c c a t t c c a g t t a a g a a a a t a t g a t a g c a t c t t g t t c t a t c a g t a g t a c a a a t t t g t a g a t g g t a a g g t c a a t t c t t t t a t a c t a t c g t a g a a c a a g a

2760 t a g t c t t t t t c t t a a t a g g g a c a t a a a g c c c a c a a a t a a a a a t a t g c c t g a a g a a t g g g a a t c a g a a a a a g a a t t a t c c c t g t a t t t c g g g t g t t t a t t t t t a t a c g g a c t t c t t a c c c t

2820 c a g g c a t t g g g c a t t g t c c a t g c c t a g t a a a g t a c t c c a a g a a c c t a t t t g t a t a c t a g a g t c c g t a a c c c g t a a c a g g t a c g g a t c a t t t c a t g a g g t t c t t g g a t a a a c a t a t g a t c t

2880 t g a c a c a a t g t c a a t g t c t g t g t a c a a c t g c c a a c t g g g a t g c a a g a c a c t g c c c a t g c c a c t g t g t t a c a g t t a c a g a c a c a t g t t g a c g g t t g a c c c t a c g t t c t g t g a c g g g t a c g g

2940 a a t c a t c c t g a a a a g c a g c t a t a a a a a g c a g g a a g c t a c t c t g c a c c t t g t c a g t g a g g t t t a g t a g g a c t t t t c g t c g a t a t t t t t c g t c c t t c g a t g a g a c g t g g a a c a g t c a c t c c a

NcoIPacI 3000 1 M 1a V 5 SKGEEL mTurquoise2 Kozak sequence MVSKGEEL *LNILGPGGHHALALLQ c c a g a t a c c t a c a g a t t a a t t a a c c c g g g g c c a c c a t g g t g a g c a a g g g c g a g g a g c t g t g g t c t a t g g a t g t c t a a t t a a t t g g g c c c c g g t g g t a c c a c t c g t t c c c g c t c c t c g a c a

3060 10 15 20 25 FTGVVPILVELDGDVNGHKF mTurquoise2 G A C C A G C T C G A C C T G C C G C T G C EGFP-N FTGVVPILVELDGDVNGHKF EGPHHGDQDLQVAVYVAVLE t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t

3120 30 35 40 45 SVSGEGEGDATYGKLTLKFI mTurquoise2 SVSGEGEGDATYGKLTLKFI AHGALALAIGGVALQGQLED g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a

3180 50 55 60 65 CTTGKLPVPWPTLVTTLSWG mTurquoise2 CTTGKLPVPWPTLVTTLSWG AGGALQGHGPGGEHGGQGPA g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g t c c t g g g g c g c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c a g g a c c c c g c

3240 70 75 80 85 VQCFARYPDHMKQHDFFKSA mTurquoise2 VQCFARYPDHMKQHDFFKSA HLAEGAVGVVHLLVVEELGG t g c a g t g c t t c g c c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a a c g t c a c g a a g c g g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t

3300 90 95 100 105 MPEGYVQERTIFFKDDGNYK mTurquoise2 C T G C T G C C G T T G A T G T T C T EXFP-R MPEGYVQERTIFFKDDGNYK HGFAVDLLAGDEELVVAVVL t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t

3360 110 115 120 125 TRAEVKFEGDTLVNRIELKG mTurquoise2 G EXFP-R TRAEVKFEGDTLVNRIELKG GAGLHLELAVGQHVADLQLA c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t

3420 130 135 140 145 IDFKEDGNILGHKLEYNYFS mTurquoise2 IDFKEDGNILGHKLEYNYFS DVELLVAVDQPVLQLVVVKA t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c t t t a g c g a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g a a a t c g c

Tth111IPflFI 3480 150 155 160 165 DNVYITADKQKNGIKANFKI mTurquoise2 DNVYITADKQKNGIKANFKI VVDIDGGVLLLVADLGVELD a c a a c g t c t a t a t c a c c g c c g a c a a g c a g a a g a a c g g c a t c a a g g c c a a c t t c a a g a t c c t g t t g c a g a t a t a g t g g c g g c t g t t c g t c t t c t t g c c g t a g t t c c g g t t g a a g t t c t a g g

3540 170 175 180 185 RHNIEDGGVQLADHYQQNTP mTurquoise2 RHNIEDGGVQLADHYQQNTP AVVDLVAAHLEGVVVLLVGG g c c a c a a c a t c g a g g a c g g c g g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a c g g t g t t g t a g c t c c t g c c g c c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t

BlpI 3600 190 195 200 205 IGDGPVLLPDNHYLSTQSKL mTurquoise2 IGDGPVLLPDNHYLSTQSKL DAVAGHQQGVVVVQAGLGLQ t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c a a g c t g a a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g t t c g a c t

3660 210 215 220 225 SKDPNEKRDHMVLLEFVTAA mTurquoise2 C A T G G T C C T G C T G G A G T T C G T G EGFP-C SKDPNEKRDHMVLLEFVTAA AFVGVLLAIVHDQQLEHGGG g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c

3720 230 235 GITLGMDELYK VYGSG 1 ATNF mTurquoise2 P2A GITLGMDELYKVYGSGATNF PDSEAHVLQVLYVSASSSVE g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g g t a t a c g g a a g c g g a g c t a c t a a c t t c a c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c c a t a t g c c t t c g c c t c g a t g a t t g a a g t

BmtINheIBmgBI 3780 5 10 15 SLLKQAGDVEENPGP AS 1 MA SV40... P P2A FLPo SLLKQAGDVEENPGPASMAP AQQLLSSVHLLVRSRSAHSR g c c t g c t g a a g c a g g c t g g a g a c g t g g a g g a g a a c c c t g g a c c t g c t a g c a t g g c t c c t a c g g a c g a c t t c g t c c g a c c t c t g c a c c t c c t c t t g g g a c c t g g a c g a t c g t a c c g a g g a t

3840 5 SV40 NLS KKKRKV 10 15 20 MSQFDILCKTPPKV FLPo KKKRKVMSQFDILCKTPPKV LLLPLHHALEVDQALGGGLH a g a a g a a g a g g a a g g t g a t g a g c c a g t t c g a c a t c c t g t g c a a g a c c c c c c c c a a g g t g c t c t t c t t c t c c t t c c a c t a c t c g g t c a a g c t g t a g g a c a c g t t c t g g g g g g g g t t c c a c g

3900 25 30 35 40 LVRQFVERFERPSGEKIASC FLPo LVRQFVERFERPSGEKIASC QHPLEHLSELPGAALLDGAT t g g t g c g g c a g t t c g t g g a g a g a t t c g a g a g g c c c a g c g g c g a g a a g a t c g c c a g c t g t g a c c a c g c c g t c a a g c a c c t c t c t a a g c t c t c c g g g t c g c c g c t c t t c t a g c g g t c g a c a c

3960 45 50 55 60 AAELTYLCWMITHNGTAIKR FLPo AAELTYLCWMITHNGTAIKR GGLQGVQAPHDGVVAGGDLP c c g c c g a g c t g a c c t a c c t g t g c t g g a t g a t c a c c c a c a a c g g c a c c g c c a t c a a g a g g g g g c g g c t c g a c t g g a t g g a c a c g a c c t a c t a g t g g g t g t t g c c g t g g c g g t a g t t c t c c c

4020 65 70 75 80 ATFMSYNTIISNSLSFDIVN FLPo ATFMSYNTIISNSLSFDIVN GGEHAVVGDDAVAQAEVDHV c c a c c t t c a t g a g c t a c a a c a c c a t c a t c a g c a a c a g c c t g a g c t t c g a c a t c g t g a a c a g g t g g a a g t a c t c g a t g t t g t g g t a g t a g t c g t t g t c g g a c t c g a a g c t g t a g c a c t t g t

4080 85 90 95 100 KSLQFKYKTQKATILEASLK FLPo KSLQFKYKTQKATILEASLK LAQLELVLGLLGGDQLGAQL a g a g c c t g c a g t t c a a g t a c a a g a c c c a g a a g g c c a c c a t c c t g g a g g c c a g c c t g a a g a t c t c g g a c g t c a a g t t c a t g t t c t g g g t c t t c c g g t g g t a g g a c c t c c g g t c g g a c t t c t

4140 105 110 115 120 KLIPAWEFTIIPYNGQKHQS FLPo KLIPAWEFTIIPYNGQKHQS LQDGGPLEGDDRVVALLVLA a g c t g a t c c c c g c c t g g g a g t t c a c c a t c a t c c c t t a c a a c g g c c a g a a g c a c c a g a g c g t c g a c t a g g g g c g g a c c c t c a a g t g g t a g t a g g g a a t g t t g c c g g t c t t c g t g g t c t c g c

4200 125 130 135 140 DITDIVSSLQLQFESSEEAD FLPo DITDIVSSLQLQFESSEEAD VDGVDHGAQLQLELAALLGV a c a t c a c c g a c a t c g t g t c c a g c c t g c a g c t g c a g t t c g a g a g c a g c g a g g a g g c c g a c a t g t a g t g g c t g t a g c a c a g g t c g g a c g t c g a c g t c a a g c t c t c g t c g c t c c t c c g g c t g t

4260 145 150 155 160 KGNSHSKKMLKALLSEGESI FLPo KGNSHSKKMLKALLSEGESI LAVAVALLHQLGQQGLALAD a g g g c a a c a g c c a c a g c a a g a a g a t g c t g a a g g c c c t g c t g t c c g a g g g c g a g a g c a t c t t c c c g t t g t c g g t g t c g t t c t t c t a c g a c t t c c g g g a c g a c a g g c t c c c g c t c t c g t a g a

4320 165 170 175 180 WEITEKILNSFEYTSRFTKT FLPo WEITEKILNSFEYTSRFTKT PLDGLLDQVAELVGAPEGLG g g g a g a t c a c c g a g a a g a t c c t g a a c a g c t t c g a g t a c a c c a g c a g g t t c a c c a a g a c c a c c c t c t a g t g g c t c t t c t a g g a c t t g t c g a a g c t c a t g t g g t c g t c c a a g t g g t t c t g g t

4380 185 190 195 200 KTLYQFLFLATFINCGRFSD FLPo KTLYQFLFLATFINCGRFSD LGQVLEQEQGCEDVAAPEAV a g a c c c t g t a c c a g t t c c t g t t c c t g g c c a c a t t c a t c a a c t g c g g c a g g t t c a g c g a c a t c t g g g a c a t g g t c a a g g a c a a g g a c c g g t g t a a g t a g t t g a c g c c g t c c a a g t c g c t g t

4440 205 210 215 220 IKNVDPKSFKLVQNKYLGVI FLPo IKNVDPKSFKLVQNKYLGVI DLVHVGLAELQHLVLVQAHD t c a a g a a c g t g g a c c c c a a g a g c t t c a a g c t g g t g c a g a a c a a g t a c c t g g g c g t g a t c a a g t t c t t g c a c c t g g g g t t c t c g a a g t t c g a c c a c g t c t t g t t c a t g g a c c c g c a c t a g t

BstEII 4500 225 230 235 240 IQCLVTETKTSVSRHIYFFS FLPo IQCLVTETKTSVSRHIYFFS NLAQHGLGLCAHGPVDVKEA t t c a g t g c c t g g t g a c c g a g a c c a a g a c a a g c g t g t c c a g g c a c a t c t a c t t t t t c a g c g a a g t c a c g g a c c a c t g g c t c t g g t t c t g t t c g c a c a g g t c c g t g t a g a t g a a a a a g t c g c

Bsu36I 4560 245 250 255 260 ARGRIDPLVYLDEFLRNSEP FLPo ARGRIDPLVYLDEFLRNSEP GSAPDVGQHVQVLEQPVALG c c a g a g g c a g g a t c g a c c c c c t g g t g t a c c t g g a c g a g t t c c t g a g g a a c a g c g a g c c c g g g t c t c c g t c c t a g c t g g g g g a c c a c a t g g a c c t g c t c a a g g a c t c c t t g t c g c t c g g g c

4620 265 270 275 280 VLKRVNRTGNSSSNKQEYQL FLPo VLKRVNRTGNSSSNKQEYQL HQLSHVPGAVAAAVLLLVLQ t g c t g a a g a g a g t g a a c a g g a c c g g c a a c a g c a g c a g c a a c a a g c a g g a g t a c c a g c t g c a c g a c t t c t c t c a c t t g t c c t g g c c g t t g t c g t c g t c g t t g t t c g t c c t c a t g g t c g a c g

SexAI*CsiI 4680 285 290 295 300 LKDNLVRSYNKALKKNAPYP FLPo LKDNLVRSYNKALKKNAPYP QLVVQHAAVVLGQLLVGGVG t g a a g g a c a a c c t g g t g c g c a g c t a c a a c a a g g c c c t g a a g a a g a a c g c c c c c t a c c c c a a c t t c c t g t t g g a c c a c g c g t c g a t g t t g t t c c g g g a c t t c t t c t t g c g g g g g a t g g g g t

4740 305 310 315 320 IFAIKNGPKSHIGRHLMTSF FLPo IFAIKNGPKSHIGRHLMTSF DESDLVARLAVDAPVQHGAK t c t t c g c t a t c a a g a a c g g c c c t a a g a g c c a c a t c g g c a g g c a c c t g a t g a c c a g c t t t c a g a a g c g a t a g t t c t t g c c g g g a t t c t c g g t g t a g c c g t c c g t g g a c t a c t g g t c g a a a g

4800 325 330 335 340 LSMKGLTELTNVVGNWSDKR FLPo LSMKGLTELTNVVGNWSDKR QAHLAQGLQCVHHAVPAVLP t g a g c a t g a a g g g c c t g a c c g a g c t g a c a a a c g t g g t g g g c a a c t g g a g c g a c a a g a g g g a c t c g t a c t t c c c g g a c t g g c t c g a c t g t t t g c a c c a c c c g t t g a c c t c g c t g t t c t c c c

4860 345 350 355 360 ASAVARTTYTHQITAIPDHY FLPo ASAVARTTYTHQITAIPDHY GGGHGPGGVGVLDGGDGVVV c c t c c g c c g t g g c c a g g a c c a c c t a c a c c c a c c a g a t c a c c g c c a t c c c c g a c c a c t a c t g g a g g c g g c a c c g g t c c t g g t g g a t g t g g g t g g t c t a g t g g c g g t a g g g g c t g g t g a t g a

4920 365 370 375 380 FALVSRYYAYDPISKEMIAL FLPo FALVSRYYAYDPISKEMIAL EGQHGPVVGVVGDALLHDGQ t c g c c c t g g t g t c c a g g t a c t a c g c c t a c g a c c c c a t c a g c a a g g a g a t g a t c g c c c t g a a g c g g g a c c a c a g g t c c a t g a t g c g g a t g c t g g g g t a g t c g t t c c t c t a c t a g c g g g a c t

4980 385 390 395 400 KDETNPIEEWQHIEQLKGSA FLPo KDETNPIEEWQHIEQLKGSA LVLGVGDLLPLVDLLQLAAG a g g a c g a g a c c a a c c c c a t c g a g g a g t g g c a g c a c a t c g a g c a g c t g a a g g g c a g c g c c g t c c t g c t c t g g t t g g g g t a g c t c c t c a c c g t c g t g t a g c t c g t c g a c t t c c c g t c g c g g c

5040 405 410 415 420 EGSIRYPAWNGIISQEVLDY FLPo EGSIRYPAWNGIISQEVLDY LAADSVGGPVADDALLHQVV a g g g c a g c a t c a g a t a c c c c g c c t g g a a c g g c a t c a t c a g c c a g g a g g t g c t g g a c t a c c t c c c g t c g t a g t c t a t g g g g c g g a c c t t g c c g t a g t a g t c g g t c c t c c a c g a c c t g a t g g

NsiI 5100 425 430 LSSYINRRI* FLPo LSSYINRRI* QAAVDVPPDLHM t g a g c a g c t a c a t c a a c a g g c g g a t c t a a a t g c a t c a g c c a t a c c a c a t t t g t a g a g g t t a c t c g t c g a t g t a g t t g t c c g c c t a g a t t t a c g t a g t c g g t a t g g t g t a a a c a t c t c c a a

5160 t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t

5220 SV40 poly(A) signal C G T T A T C G T A G SV40pA-R a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g

5280 SV40 poly(A) signal T G T T T A A A G SV40pA-R a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g

EagINotINgoMIVNaeIFseIMluI 5340 SV40 poly(A) signal a t c a a t g t a t c t t a a c g c g t a a a t t g t a a g c g g g c c g g c c a t a t t t g t c a c c g g a c t g c g t a g t t a c a t a g a a t t g c g c a t t t a a c a t t c g c c c g g c c g g t a t a a a c a g t g g c c t g a c g c

BsaBI* 5400 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev g c c g c g a c t c t a g a t c a t a a t c a g c c c g g g g g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c g g c g c t g a g a t c t a g t a t t a g t c g g g c c c c c t a g t c g g a g c t g a c a c g g a a g a t c a a c g

5460 bGH poly(A) signal c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g

5520 bGH poly(A) signal a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a

BbsI 5580 bGH poly(A) signal a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c

5640 bGH poly(A) signal lox2272 c a t g c t g g g g a t g c g g t g g g c t c t a t g g a a c c a g c t g g g g c g c g a t a a c t t c g t a t a g g a g t a c g a c c c c t a c g c c a c c c g a g a t a c c t t g g t c g a c c c c g c g c t a t t g a a g c a t a t c c t

XcmI 5700 lox2272 g a c t t t a t a c g a a g t t a t g c g c c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c c t g a a a t a t g c t t c a a t a c g c g g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g

5760 t t a t c a t g t c t g g a t c c c a t c a c a a a g c t c t g a c c t c a a t c c t a t a g a a a g g a g g a a t g a a a t a g t a c a g a c c t a g g g t a g t g t t t c g a g a c t g g a g t t a g g a t a t c t t t c c t c c t t a c t

5820 g c c a a a a t t c a c c c a a c t t a t t g t g g g a a g c t t g t g g a a g g c t a c t c g a a a t g t t t g a c c c g g t t t t a a g t g g g t t g a a t a a c a c c c t t c g a a c a c c t t c c g a t g a g c t t t a c a a a c t g g

5880 c a a g t t a a a c a a t t t a a a g g c a a t g c t a c c a a a t a c t a a t t g a g t g t a t g t t a a c t t c t g g t t c a a t t t g t t a a a t t t c c g t t a c g a t g g t t t a t g a t t a a c t c a c a t a c a a t t g a a g a c

5940 a c c c a c t g g g a a t g t g a t g a a a g a a a t a a a a g c t g a a a t g a a t c a t t c t c t c t a c t a t t a t g g g t g a c c c t t a c a c t a c t t t c t t t a t t t t c g a c t t t a c t t a g t a a g a g a g a t g a t a a t

6000 t t c t g a t a t t t c a c a t t c t t a a a a t a a a g t g g t g a t c c t a a c t g a c c t t a a g a c a g g g a a a a g a c t a t a a a g t g t a a g a a t t t t a t t t c a c c a c t a g g a t t g a c t g g a a t t c t g t c c c t t

6060 t c t t t a c t c g g a t t a a a t g t c a g g a a t t g t g a a a a a g t g a g t t t a a a t g t a t t t g g c t a a a g a a a t g a g c c t a a t t t a c a g t c c t t a a c a c t t t t t c a c t c a a a t t t a c a t a a a c c g a t t

SbfI 6120 g g t g t a t g t a a a c t t c c g a c t t c a a c t g t a g g g g a t c c t c t a g a g t c g a c c t g c a g g c a t c c a c a t a c a t t t g a a g g c t g a a g t t g a c a t c c c c t a g g a g a t c t c a g c t g g a c g t c c g t a

6180 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse g c a a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a c g t t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t

6240 -10 -35 lac operator lac promoter CAP binding site a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c

6300 CAP binding site a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c

6360 t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g

BspQISapI 6420 G A G C G A G T G A C T G A G C G A L4440 t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t

6480 t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c

PciI 6540 a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c

6600 ori t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c

6660 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c

6720 ori c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t

6780 ori a g c g t g g c g c t t t c t c a a t g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c g c a c c g c g a a a g a g t t a c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g

6840 ori t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a

6900 ori a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a

6960 ori g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c

7020 ori c c t a a c t a c g g c t a c a c t a g a a g g a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t g g a t t g a t g c c g a t g t g a t c t t c c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a

7080 ori a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a

7140 ori g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a

7200 t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c

7260 g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a

7320 285 *WHKIL AmpR *WHKIL a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a

7380 280 275 270 265 SAGIEAIQRNREDMTAQSGT AmpR SAGIEAIQRNREDMTAQSGT g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g

7440 260 255 250 245 TYIVVIRSPKGDPGLAAIIG AmpR TYIVVIRSPKGDPGLAAIIG g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c

7500 240 235 230 225 RSGREGAGSKDAIFWGAPLA AmpR RSGREGAGSKDAIFWGAPLA c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g

7560 220 215 210 205 SRLLPGAVKDAEMWDILQQR AmpR SRLLPGAVKDAEMWDILQQR g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c

7620 200 195 190 185 SALTLLEGTLLKRLTTAMAV AmpR SALTLLEGTLLKRLTTAMAV g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t

7680 180 175 170 165 PMTTDREDNPIAENLEPEWR AmpR PMTTDREDNPIAENLEPEWR g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t

7740 160 155 150 145 DLRTVHDGMNHLFATLEKPG AmpR DLRTVHDGMNHLFATLEKPG t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a

7800 140 135 130 125 GITTLLLNAATNDSMTIAAS AmpR GITTLLLNAATNDSMTIAAS c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c

7860 120 115 110 105 CLERVTMGDTLHKETVPSYE AmpR CLERVTMGDTLHKETVPSYE c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t

7920 100 95 90 85 VLDNQSYHIRRGLQEQGADI AmpR VLDNQSYHIRRGLQEQGADI a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t

7980 80 75 70 65 RSLVAGCLLVKFTSMMPFRE AmpR A T A A T A C C G C G C C A C A T A G C Amp-R RSLVAGCLLVKFTSMMPFRE c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a

8040 60 55 50 45 EPRFSELIKGSNLDLEIYGV AmpR EPRFSELIKGSNLDLEIYGV t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a

8100 40 35 30 25 RAGLQDEADKVKVLTEPH signal sequ... AF AmpR RAGLQDEADKVKVLTEPHAF c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t

8160 20 15 10 5 signal sequence VPLCFAAFFPILAVRFHQIS AmpR VPLCFAAFFPILAVRFHQIS a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g

SspI 8220 1 M AmpR AmpR promoter M a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t

8280 AmpR promoter t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t

ZraIAatII 8340 A A T A G G pBRforEco a a a g t g c c a c c t g a c g t c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a a t a g g t t t c a c g g t g g a c t g c a g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t t a t c c

3ʹ 5ʹ 8362 C G T A T C A C G A G G C pBRforEco c g t a t c a c g a g g c c c t t t c g t c g c a t a g t g c t c c g g g a a a g c a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.