Addgene pMLLKA-J72020 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pMLLKA-J72020


Map View

BglII (0) EcoRI (2918) ApaI (2781) PspOMI (2777) BtgI - NcoI - StyI (2773) PsiI (2618) SnaBI (2409) PflMI (2202) BsmBI - Esp3I (1955) AsiSI (1939) EcoNI (1851) SmaI (1813) TspMI - XmaI (1811) BspDI - ClaI (1630) Kan-R (1581 .. 1600) NruI (1596) pENTR-R (1497 .. 1516) AflII (4) BamHI (75) Bsu36I (91) L4440 (101 .. 118) BciVI (306) ApaLI (471) MspA1I (507) BpuEI (539) XmnI (543) Amp-R (574 .. 593) ScaI (662) EaeI (750) FspI (920) NmeAIII (996) EciI (1001) BglI (1025) BsaI (1077) BmrI (1103) AhdI (1143) BanI (1190) BbsI - PaeR7I - XhoI (1326) EcoP15I (1329) PstI (1336) DraIII (1408) pMLLKA-J72020 2927 bp

Sequence View

BglIIAflII 60 1 5 YPYDV HA g a t c t t a a g c a c c c a t a g a a t a c a c a c c t g g a g g a a t a a t g t c t t a c c c a t a c g a c g t c c c t a g a a t t c g t g g g t a t c t t a t g t g t g g a c c t c c t t a t t a c a g a a t g g g t a t g c t g c a g g

Bsu36IBamHI 120 PDYA (in frame with HA) GGS* HA G A G C G A G T G A C T G A G C G A L4440 c a g a c t a c g c t g g g g g a t c c t a a c t c g c t c c t c a g g c t t c c t c g c t c a c t g a c t c g c t g c g t c t g a t g c g a c c c c c t a g g a t t g a g c g a g g a g t c c g a a g g a g c g a g t g a c t g a g c g a c g

180 attB2 g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t c a a t t c g a c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a g t t a a g c t

240 attB2 c c c a g c t t t c t t g t a c a a a g t g g t t g a t c g g a t c t c t t a g a c g t c a g g t g g c a c t t t t c g g g g t c g a a a g a a c a t g t t t c a c c a a c t a g c c t a g a g a a t c t g c a g t c c a c c g t g a a a a g c

300 AmpR promoter g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g

BciVI 360 1 signal... MS AmpR promoter AmpR MS g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c

420 5 10 15 20 signal sequence IQHFRVALIPFFAAFCLPVF AmpR IQHFRVALIPFFAAFCLPVF t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a

ApaLI 480 signa... A 25 30 35 40 HPETLVKVKDAEDQLGARV AmpR AHPETLVKVKDAEDQLGARV t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c a g a g g a t c a g t t g g g t g c a c g a g t a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g t c t c c t a g t c a a c c c a c g t g c t c a

BpuEIMspA1I 540 45 50 55 60 GYIELDLNSGKILESFRPEE AmpR GYIELDLNSGKILESFRPEE g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t

XmnI 600 65 70 75 80 RFPMMSTFKVLLCGAVLSRI AmpR C G A T A C A C C G C G C C A T A A T A Amp-R RFPMMSTFKVLLCGAVLSRI a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a

660 85 90 95 100 DAGQEQLGRRIHYSQNDLVE AmpR DAGQEQLGRRIHYSQNDLVE t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t

ScaI 720 105 110 115 120 YSPVTEKHLTDGMTVRELCS AmpR YSPVTEKHLTDGMTVRELCS g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c

EaeI 780 125 130 135 140 AAITMSDNTAANLLLTTIGG AmpR AAITMSDNTAANLLLTTIGG t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c

840 145 150 155 160 PKELTAFLHNMGDHVTRLDR AmpR PKELTAFLHNMGDHVTRLDR a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c

900 165 170 175 180 WEPELNEAIPNDERDTTMPV AmpR WEPELNEAIPNDERDTTMPV t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a

FspI 960 185 190 195 200 AMATTLRKLLTGELLTLASR AmpR AMATTLRKLLTGELLTLASR a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c

NmeAIIIEciI 1020 205 210 215 220 QQLIDWMEADKVAGPLLRSA AmpR QQLIDWMEADKVAGPLLRSA g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g

BsaIBglI 1080 225 230 235 240 LPAGWFIADKSGAGERGSRG AmpR LPAGWFIADKSGAGERGSRG c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c

BmrI 1140 245 250 255 260 IIAALGPDGKPSRIVVIYTT AmpR IIAALGPDGKPSRIVVIYTT t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g

BanIAhdI 1200 265 270 275 280 GSQATMDERNRQIAEIGASL AmpR GSQATMDERNRQIAEIGASL g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a

1260 285 IKHW* AmpR IKHW* g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t

1320 a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c g g a t c t c t g a t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g c c t a g a g a c t

PstIPaeR7IXhoIBbsIEcoP15I 1380 a g a c t c t c g a g c t g c a g g a c t c a c a g c t c c t c g g a t c t g c t g a t c c t t c a a c t c a g c a a a t c t g a g a g c t c g a c g t c c t g a g t g t c g a g g a g c c t a g a c g a c t a g g a a g t t g a g t c g t t t

DraIII 1440 a g t t c g a t t t a t t c a a c a a a g c c a c g t t g t g t c t c a a a a t c t c t g a t g t t a c a t t g c a c a t c a a g c t a a a t a a g t t g t t t c g g t g c a a c a c a g a g t t t t a g a g a c t a c a a t g t a a c g t g t

1500 G T T C pENTR-R a g a t a a a a a t a t a t c a t c a t g a a c a a t a a a a c t g t c t g c t t a c a t a a a c a g t a a t a c a a g t c t a t t t t t a t a t a g t a g t a c t t g t t a t t t t g a c a g a c g a a t g t a t t t g t c a t t a t g t t c

1560 1 5 10 15 MSHIQRETSCSRPRLNSN KanR 1 5 10 15 MSHIQRETSCSRPRLNSN KanR C C C A C A A T A C T C G G T A pENTR-R MSHIQRETSCSRPRLNSN g g g t g t t a t g a g c c a t a t t c a a c g g g a a a c g t c t t g c t c c a g g c c g c g a t t a a a t t c c a a c c c a c a a t a c t c g g t a t a a g t t g c c c t t t g c a g a a c g a g g t c c g g c g c t a a t t t a a g g t t

NruI 1620 20 25 30 35 MDADLYGYKWARDNVGQSGA KanR 20 25 30 35 MDADLYGYKWARDNVGQSGA KanR C C A T A T T T A C C C G A G C G C T A Kan-R MDADLYGYKWARDNVGQSGA c a t g g a t g c t g a t t t a t a t g g g t a t a a a t g g g c t c g c g a t a a t g t c g g g c a a t c a g g t g c g t a c c t a c g a c t a a a t a t a c c c a t a t t t a c c c g a g c g c t a t t a c a g c c c g t t a g t c c a c g

BspDIClaI 1680 40 45 50 55 TIYRLYGKPDAPELFLKHGK KanR 40 45 50 55 TIYRLYGKPDAPELFLKHGK KanR TIYRLYGKPDAPELFLKHGK g a c a a t c t a t c g a t t g t a t g g g a a g c c c g a t g c g c c a g a g t t g t t t c t g a a a c a t g g c a a c t g t t a g a t a g c t a a c a t a c c c t t c g g g c t a c g c g g t c t c a a c a a a g a c t t t g t a c c g t t

1740 60 65 70 75 GSVANDVTDEMVRLNWLTEF KanR 60 65 70 75 GSVANDVTDEMVRLNWLTEF KanR GSVANDVTDEMVRLNWLTEF a g g t a g c g t t g c c a a t g a t g t t a c a g a t g a g a t g g t c a g a c t a a a c t g g c t g a c g g a a t t t c c a t c g c a a c g g t t a c t a c a a t g t c t a c t c t a c c a g t c t g a t t t g a c c g a c t g c c t t a a

1800 80 85 90 95 MPLPTIKHFIRTPDDAWLLT KanR 80 85 90 95 MPLPTIKHFIRTPDDAWLLT KanR MPLPTIKHFIRTPDDAWLLT t a t g c c t c t t c c g a c c a t c a a g c a t t t t a t c c g t a c t c c t g a t g a t g c a t g g t t a c t c a c a t a c g g a g a a g g c t g g t a g t t c g t a a a a t a g g c a t g a g g a c t a c t a c g t a c c a a t g a g t g

EcoNITspMIXmaISmaI 1860 100 105 110 115 TAIPGKTAFQVLEEYPDSGE KanR 100 105 110 115 TAIPGKTAFQVLEEYPDSGE KanR TAIPGKTAFQVLEEYPDSGE c a c t g c g a t c c c c g g g a a a a c a g c a t t c c a g g t a t t a g a a g a a t a t c c t g a t t c a g g t g a g t g a c g c t a g g g g c c c t t t t g t c g t a a g g t c c a t a a t c t t c t t a t a g g a c t a a g t c c a c t

1920 120 125 130 135 NIVDALAVFLRRLHSIPVCN KanR 120 125 130 135 NIVDALAVFLRRLHSIPVCN KanR NIVDALAVFLRRLHSIPVCN a a a t a t t g t t g a t g c g c t g g c a g t g t t c c t g c g c c g g t t g c a t t c g a t t c c t g t t t g t a a t t t a t a a c a a c t a c g c g a c c g t c a c a a g g a c g c g g c c a a c g t a a g c t a a g g a c a a a c a t t

BsmBIEsp3IAsiSI 1980 140 145 150 155 CPFNSDRVFRLAQAQSRMNN KanR 140 145 150 155 CPFNSDRVFRLAQAQSRMNN KanR CPFNSDRVFRLAQAQSRMNN t t g t c c t t t t a a c a g c g a t c g c g t a t t t c g t c t c g c t c a g g c g c a a t c a c g a a t g a a t a a a a c a g g a a a a t t g t c g c t a g c g c a t a a a g c a g a g c g a g t c c g c g t t a g t g c t t a c t t a t t

2040 160 165 170 175 GLVDASDFDDERNGWPVEQV KanR 160 165 170 175 GLVDASDFDDERNGWPVEQV KanR GLVDASDFDDERNGWPVEQV c g g t t t g g t t g a t g c g a g t g a t t t t g a t g a c g a g c g t a a t g g c t g g c c t g t t g a a c a a g t g c c a a a c c a a c t a c g c t c a c t a a a a c t a c t g c t c g c a t t a c c g a c c g g a c a a c t t g t t c a

2100 180 185 190 195 WKEMHKLLPFSPDSVVTHGD KanR 180 185 190 195 WKEMHKLLPFSPDSVVTHGD KanR WKEMHKLLPFSPDSVVTHGD c t g g a a a g a a a t g c a t a a g c t t t t g c c a t t c t c a c c g g a t t c a g t c g t c a c t c a t g g t g a g a c c t t t c t t t a c g t a t t c g a a a a c g g t a a g a g t g g c c t a a g t c a g c a g t g a g t a c c a c t

2160 200 205 210 215 FSLDNLIFDEGKLIGCIDVG KanR 200 205 210 215 FSLDNLIFDEGKLIGCIDVG KanR FSLDNLIFDEGKLIGCIDVG t t t c t c a c t t g a t a a c c t t a t t t t t g a c g a g g g g a a a t t a a t a g g t t g t a t t g a t g t t g g a a a g a g t g a a c t a t t g g a a t a a a a a c t g c t c c c c t t t a a t t a t c c a a c a t a a c t a c a a c c

PflMI 2220 220 225 230 235 RVGIADRYQDLAILWNCLGE KanR 220 225 230 235 RVGIADRYQDLAILWNCLGE KanR RVGIADRYQDLAILWNCLGE a c g a g t c g g a a t c g c a g a c c g a t a c c a g g a t c t t g c c a t c c t a t g g a a c t g c c t c g g t g a t g c t c a g c c t t a g c g t c t g g c t a t g g t c c t a g a a c g g t a g g a t a c c t t g a c g g a g c c a c t

2280 240 245 250 255 FSPSLQKRLFQKYGIDNPDM KanR 240 245 250 255 FSPSLQKRLFQKYGIDNPDM KanR FSPSLQKRLFQKYGIDNPDM g t t t t c t c c t t c a t t a c a g a a a c g g c t t t t t c a a a a a t a t g g t a t t g a t a a t c c t g a t a t c a a a a g a g g a a g t a a t g t c t t t g c c g a a a a a g t t t t t a t a c c a t a a c t a t t a g g a c t a t a

2340 260 265 270 NKLQFHLMLDEFF* KanR 260 265 270 NKLQFHLMLDEFF* KanR NKLQFHLMLDEFF* g a a t a a a t t g c a g t t t c a t t t g a t g c t c g a t g a g t t t t t c t a a t c a g a a t t g g t t a a t t g c t t a t t t a a c g t c a a a g t a a a c t a c g a g c t a c t c a a a a a g a t t a g t c t t a a c c a a t t a a c

2400 g t t g t a a c a c t g g c a g a g c a t t a c g c t g a c t t g a c g g g a g g a t c t a g c a g t t c a a c c t g t c a a c a t t g t g a c c g t c t c g t a a t g c g a c t g a a c t g c c c t c c t a g a t c g t c a a g t t g g a c a

SnaBI 2460 R6K γ ori t g a t a g t a c g t a c t a a g c t c t c a t g t t t c a c g t a c t a a g c t c t c a t g t t t a a c g t a c t a a a c t a t c a t g c a t g a t t c g a g a g t a c a a a g t g c a t g a t t c g a g a g t a c a a a t t g c a t g a t t

2520 R6K γ ori g c t c t c a t g t t t a a c g a a c t a a a c c c t c a t g g c t a a c g t a c t a a g c t c t c a t g g c t a a c g c g a g a g t a c a a a t t g c t t g a t t t g g g a g t a c c g a t t g c a t g a t t c g a g a g t a c c g a t t g c

2580 R6K γ ori t a c t a a g c t c t c a t g t t t c a c g t a c t a a g c t c t c a t g t t t g a a c a a t a a a a t t a a t a t a a a t g a t t c g a g a g t a c a a a g t g c a t g a t t c g a g a g t a c a a a c t t g t t a t t t t a a t t a t a t t

PsiI 2640 R6K γ ori a t c a g c a a c t t a a a t a g c c t c t a a g g t t t t a a g t t t t a t a a g a a a a a a a a g a a t a t a t a a t a g t c g t t g a a t t t a t c g g a g a t t c c a a a a t t c a a a a t a t t c t t t t t t t t c t t a t a t a t t

2700 R6K γ ori g g c t t t t a a a g c t t t t a a g g t t t a a c g g t t g t g g a c a a c a a g c c a g g g a t g t a a c g c a c t c c g a a a a t t t c g a a a a t t c c a a a t t g c c a a c a c c t g t t g t t c g g t c c c t a c a t t g c g t g a

2760 R6K γ ori g a g a a g c c c t t a g a g c c t c t c a a a g c a a t t t t g a g t g a c a c a g g a a c a c t t a a c g g c t g a c t c t t c g g g a a t c t c g g a g a g t t t c g t t a a a a c t c a c t g t g t c c t t g t g a a t t g c c g a c t

NcoIStyIBtgIPspOMIApaI 2820 R6K γ ori c a t g g g a a t t a g c c a t g g g c c c g t g c g a a t c a c g g a t c t c c c g a t c a a g a a t t g c c a t t a g t a c c c t t a a t c g g t a c c c g g g c a c g c t t a g t g c c t a g a g g g c t a g t t c t t a a c g g t a a t

2880 attB1 t c a a c a a g t t t g t a c a a a a a a g c a g g c t c c g a a t t g g t a t c a c g a g g c a g a a t t t c a g a t a g t t g t t c a a a c a t g t t t t t t c g t c c g a g g c t t a a c c a t a g t g c t c c g t c t t a a a g t c t a

EcoRI 2927 a a a a a a a a t c c t t a g c t t t c g c t a a g g a t c t g a a g t g g a a t t c a t g a t t t t t t t t a g g a a t c g a a a g c g a t t c c t a g a c t t c a c c t t a a g t a c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.