Addgene pMpGWB114 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pMpGWB114


Map View

RB T-DNA repeat BsiWI (10,012) BmtI (9900) NheI (9896) BspDI * - ClaI * (9818) PluTI (9115) MreI - SgrAI (9114) SfoI (9113) NarI (9112) KasI (9111) pGEX 3' (9041 .. 9063) pRS-marker (8881 .. 8900) L4440 (8783 .. 8800) pBR322ori-F (8530 .. 8549) BlpI (7311) BstEII (7162) PmeI (92) M13/pUC Forward (266 .. 288) M13 Forward (280 .. 297) M13 fwd HindIII (301) SbfI (317) MfeI (808) XbaI (1166) AbsI (1311) lac UV5 promoter CAT-R (1490 .. 1509) BglII (2084) BbvCI (2362) TspMI - XmaI (2506) SmaI - SrfI (2508) ccdB-fwd (2609 .. 2628) SalI (2765) Bsu36I (3474) BstBI (3514) Eco53kI (3783) BanII - SacI (3785) AflII (3826) MauBI (4021) M13 rev M13 Reverse (4083 .. 4099) lac operator M13/pUC Reverse (4096 .. 4118) CAP binding site AscI (4209) RsrII (5110) AsiSI (5159) ZraI (5496) AatII (5498) pMpGWB114 12,937 bp

Sequence View

5ʹ 3ʹ 60 RB T-DNA repeat t t t c a c g c c c t t t t a a a t a t c c g a t t a t t c t a a t a a a c g c t c t t t t c t c t t a g g t t t a c c a a a g t g c g g g a a a a t t t a t a g g c t a a t a a g a t t a t t t g c g a g a a a a g a g a a t c c a a a t g g

PmeI 120 RB T-DNA repeat c g c c a a t a t a t c c t g t c a a a c a c t g a t a g t t t a a a c t g a a g g c g g g a a a c g a c a a t c t g a g c g g t t a t a t a g g a c a g t t t g t g a c t a t c a a a t t t g a c t t c c g c c c t t t g c t g t t a g a c t

180 t c c a a g c t c a a g c t g c t c t a g c a t t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a a g g t t c g a g t t c g a c g a g a t c g t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t

240 t c g g t g c g g g c c t c t t c g c t a t t a c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a a g c c a c g c c c g g a g a a g c g a t a a t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t

300 M13 fwd C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward t t a a g t t g g g t a a c g c c a g g g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t g c c a a t t c a a c c c a t t g c g g t c c c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a c g g

SbfIHindIII 360 MPAGPQISLFNFRKNAN a a g c t t g c a t g c c t g c a g g t c c c c a g a t t a g c c t t t t c a a t t t c a g a a a g a a t g c t a a c c t t c g a a c g t a c g g a c g t c c a g g g g t c t a a t c g g a a a a g t t a a a g t c t t t c t t a c g a t t g g

420 PQMVREAYAAGLIKTIYPSN c a c a g a t g g t t a g a g a g g c t t a c g c a g c a g g t c t c a t c a a g a c g a t c t a c c c g a g c a a t a g t g t c t a c c a a t c t c t c c g a a t g c g t c g t c c a g a g t a g t t c t g c t a g a t g g g c t c g t t a t

480 NLQEIKYLPKKVKDAVKRFR a t c t c c a g g a a a t c a a a t a c c t t c c c a a g a a g g t t a a a g a t g c a g t c a a a a g a t t c a g g a t a g a g g t c c t t t a g t t t a t g g a a g g g t t c t t c c a a t t t c t a c g t c a g t t t t c t a a g t c c t

540 TNCIKNTEKDIFLKIRSTIP c t a a c t g c a t c a a g a a c a c a g a g a a a g a t a t a t t t c t c a a g a t c a g a a g t a c t a t t c c a g g a t t g a c g t a g t t c t t g t g t c t c t t t c t a t a t a a a g a g t t c t a g t c t t c a t g a t a a g g t c

600 VWTIQGLLHKPRQVIEIGVS t a t g g a c g a t t c a a g g c t t g c t t c a c a a a c c a a g g c a a g t a a t a g a g a t t g g a g t c t c t a a t a c c t g c t a a g t t c c g a a c g a a g t g t t t g g t t c c g t t c a t t a t c t c t a a c c t c a g a g a t

660 KKVVPTESKAMESKIQIEDL a a a a g g t a g t t c c c a c t g a a t c a a a g g c c a t g g a g t c a a a g a t t c a a a t a g a g g a c c t a a t t t t c c a t c a a g g g t g a c t t a g t t t c c g g t a c c t c a g t t t c t a a g t t t a t c t c c t g g a t t

720 TELAVKTGEQFIQSLLRLND c a g a a c t c g c c g t a a a g a c t g g c g a a c a g t t c a t a c a g a g t c t c t t a c g a c t c a a t g a c a g t c t t g a g c g g c a t t t c t g a c c g c t t g t c a a g t a t g t c t c a g a g a a t g c t g a g t t a c t g t

780 KKKIFVNMVEHDTLVYSKNI a g a a g a a a a t c t t c g t c a a c a t g g t g g a g c a c g a c a c a c t t g t c t a c t c c a a a a a t a t c a t c t t c t t t t a g a a g c a g t t g t a c c a c c t c g t g c t g t g t g a a c a g a t g a g g t t t t t a t a g t

MfeI 840 CaMV 35S promoter KDTVSEDQRAIETFQQRVIS a a g a t a c a g t c t c a g a a g a c c a a a g g g c a a t t g a g a c t t t t c a a c a a a g g g t a a t a t c c g t t c t a t g t c a g a g t c t t c t g g t t t c c c g t t a a c t c t g a a a a g t t g t t t c c c a t t a t a g g c

900 CaMV 35S promoter GNLLGFHCPAICHFIVKIVE g a a a c c t c c t c g g a t t c c a t t g c c c a g c t a t c t g t c a c t t t a t t g t g a a g a t a g t g g a a a c t t t g g a g g a g c c t a a g g t a a c g g g t c g a t a g a c a g t g a a a t a a c a c t t c t a t c a c c t t t

960 CaMV 35S promoter KEGGSYKCHHCDKGKAIVED a g g a a g g t g g c t c c t a c a a a t g c c a t c a t t g c g a t a a a g g a a a g g c c a t c g t t g a a g a t g t c c t t c c a c c g a g g a t g t t t a c g g t a g t a a c g c t a t t t c c t t t c c g g t a g c a a c t t c t a c

1020 CaMV 35S promoter ASADSGPKDGPPPTRSIVEK c c t c t g c c g a c a g t g g t c c c a a a g a t g g a c c c c c a c c c a c g a g g a g c a t c g t g g a a a a a g g g a g a c g g c t g t c a c c a g g g t t t c t a c c t g g g g g t g g g t g c t c c t c g t a g c a c c t t t t t c

1080 CaMV 35S promoter EDVPTTSSKQVD* a a g a c g t t c c a a c c a c g t c t t c a a a g c a a g t g g a t t g a t g t g a t a t c t c c a c t g a c g t a a t t c t g c a a g g t t g g t g c a g a a g t t t c g t t c a c c t a a c t a c a c t a t a g a g g t g a c t g c a t t

1140 CaMV 35S promoter g g g a t g a c g c a c a a t c c c a c t a t c c t t c g c a a g a c c c t t c c t c t a t a t a a g g a a g t t c a t c c c t a c t g c g t g t t a g g g t g a t a g g a a g c g t t c t g g g a a g g a g a t a t a t t c c t t c a a g t a

XbaI 1200 CaMV 35S promoter attR1 t t c a t t t g g a g a g a a c a c g g g g g a c t c t a g a g t t a t c a a c a a g t t t g t a c a a a a a a g c t g a a g t a a a c c t c t c t t g t g c c c c c t g a g a t c t c a a t a g t t g t t c a a a c a t g t t t t t t c g a c

1260 attR1 a a c g a g a a a c g t a a a a t g a t a t a a a t a t c a a t a t a t t a a a t t a g a t t t t g c a t a a a a a a c t t g c t c t t t g c a t t t t a c t a t a t t t a t a g t t a t a t a a t t t a a t c t a a a a c g t a t t t t t t g

AbsI 1320 attR1 a g a c t a c a t a a t a c t g t a a a a c a c a a c a t a t c c a g t c a c t a t g g c g g c c c c t c g a g g g g c t c t g a t g t a t t a t g a c a t t t t g t g t t g t a t a g g t c a g t g a t a c c g c c g g g g a g c t c c c c g

1380 -35 -10 lac UV5 promoter c g c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t a a t g t g t g g a t t t t g c g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a t t a c a c a c c t a a a a

1440 1 5 MEKKIT CmR MEKKIT g a g t t a g g a t c c g t c g a g a t t t t c a g g a g c t a a g g a a g c t a a a a t g g a g a a a a a a a t c a c c t c a a t c c t a g g c a g c t c t a a a a g t c c t c g a t t c c t t c g a t t t t a c c t c t t t t t t t a g t g

1500 10 15 20 25 GYTTVDISQWHRKEHFEAFQ CmR C T C C G T A A A G T CAT-R GYTTVDISQWHRKEHFEAFQ t g g a t a t a c c a c c g t t g a t a t a t c c c a a t g g c a t c g t a a a g a a c a t t t t g a g g c a t t t c a a c c t a t a t g g t g g c a a c t a t a t a g g g t t a c c g t a g c a t t t c t t g t a a a a c t c c g t a a a g t

1560 30 35 40 45 SVAQCTYNQTVQLDITAFLK CmR C A G T C A A C G CAT-R SVAQCTYNQTVQLDITAFLK g t c a g t t g c t c a a t g t a c c t a t a a c c a g a c c g t t c a g c t g g a t a t t a c g g c c t t t t t a a a c a g t c a a c g a g t t a c a t g g a t a t t g g t c t g g c a a g t c g a c c t a t a a t g c c g g a a a a a t t t

1620 50 55 60 65 TVKKNKHKFYPAFIHILARL CmR TVKKNKHKFYPAFIHILARL g a c c g t a a a g a a a a a t a a g c a c a a g t t t t a t c c g g c c t t t a t t c a c a t t c t t g c c c g c c t c t g g c a t t t c t t t t t a t t c g t g t t c a a a a t a g g c c g g a a a t a a g t g t a a g a a c g g g c g g a

1680 70 75 80 85 MNAHPEFRMAMKDGELVIWD CmR MNAHPEFRMAMKDGELVIWD g a t g a a t g c t c a t c c g g a a t t c c g t a t g g c a a t g a a a g a c g g t g a g c t g g t g a t a t g g g a c t a c t t a c g a g t a g g c c t t a a g g c a t a c c g t t a c t t t c t g c c a c t c g a c c a c t a t a c c c t

1740 90 95 100 105 SVHPCYTVFHEQTETFSSLW CmR SVHPCYTVFHEQTETFSSLW t a g t g t t c a c c c t t g t t a c a c c g t t t t c c a t g a g c a a a c t g a a a c g t t t t c a t c g c t c t g a t c a c a a g t g g g a a c a a t g t g g c a a a a g g t a c t c g t t t g a c t t t g c a a a a g t a g c g a g a c

1800 110 115 120 125 SEYHDDFRQFLHIYSQDVAC CmR SEYHDDFRQFLHIYSQDVAC g a g t g a a t a c c a c g a c g a t t t c c g g c a g t t t c t a c a c a t a t a t t c g c a a g a t g t g g c g t g c t c a c t t a t g g t g c t g c t a a a g g c c g t c a a a g a t g t g t a t a t a a g c g t t c t a c a c c g c a c

1860 130 135 140 145 YGENLAYFPKGFIENMFFVS CmR YGENLAYFPKGFIENMFFVS t t a c g g t g a a a a c c t g g c c t a t t t c c c t a a a g g g t t t a t t g a g a a t a t g t t t t t c g t c t c a a t g c c a c t t t t g g a c c g g a t a a a g g g a t t t c c c a a a t a a c t c t t a t a c a a a a a g c a g a g

1920 150 155 160 165 ANPWVSFTSFDLNVANMDNF CmR ANPWVSFTSFDLNVANMDNF a g c c a a t c c c t g g g t g a g t t t c a c c a g t t t t g a t t t a a a c g t g g c c a a t a t g g a c a a c t t t c g g t t a g g g a c c c a c t c a a a g t g g t c a a a a c t a a a t t t g c a c c g g t t a t a c c t g t t g a a

1980 170 175 180 185 FAPVFTMGKYYTQGDKVLMP CmR FAPVFTMGKYYTQGDKVLMP c t t c g c c c c c g t t t t c a c c a t g g g c a a a t a t t a t a c g c a a g g c g a c a a g g t g c t g a t g c c g a a g c g g g g g c a a a a g t g g t a c c c g t t t a t a a t a t g c g t t c c g c t g t t c c a c g a c t a c g g

2040 190 195 200 205 LAIQVHHAVCDGFHVGRMLN CmR LAIQVHHAVCDGFHVGRMLN g c t g g c g a t t c a g g t t c a t c a t g c c g t t t g t g a t g g c t t c c a t g t c g g c a g a a t g c t t a a c g a c c g c t a a g t c c a a g t a g t a c g g c a a a c a c t a c c g a a g g t a c a g c c g t c t t a c g a a t t

BglII 2100 210 215 220 ELQQYCDEWQGGA* CmR ELQQYCDEWQGGA* t g a a t t a c a a c a g t a c t g c g a t g a g t g g c a g g g c g g g g c g t a a a g a t c t g g a t c c g g c t t a c t t a a t g t t g t c a t g a c g c t a c t c a c c g t c c c g c c c c g c a t t t c t a g a c c t a g g c c g a a

2160 a c t a a a a g c c a g a t a a c a g t a t g c g t a t t t g c g c g c t g a t t t t t g c g g t a t a a g a a t a t a t g a t t t t c g g t c t a t t g t c a t a c g c a t a a a c g c g c g a c t a a a a a c g c c a t a t t c t t a t a t

2220 t a c t g a t a t g t a t a c c c g a a g t a t g t c a a a a a g a g g t a t g c t a t g a a g c a g c g t a t t a c a a t g a c t a t a c a t a t g g g c t t c a t a c a g t t t t t c t c c a t a c g a t a c t t c g t c g c a t a a t g t

2280 g t g a c a g t t g a c a g c g a c a g c t a t c a g t t g c t c a a g g c a t a t a t g a t g t c a a t a t c t c c g c a c t g t c a a c t g t c g c t g t c g a t a g t c a a c g a g t t c c g t a t a t a c t a c a g t t a t a g a g g c

2340 g t c t g g t a a g c a c a a c c a t g c a g a a t g a a g c c c g t c g t c t g c g t g c c g a a c g c t g g a a a g c a g a c c a t t c g t g t t g g t a c g t c t t a c t t c g g g c a g c a g a c g c a c g g c t t g c g a c c t t t c

BbvCI 2400 c g g a a a a t c a g g a a g g g a t g g c t g a g g t c g c c c g g t t t a t t g a a a t g a a c g g c t c t t t t g g c c t t t t a g t c c t t c c c t a c c g a c t c c a g c g g g c c a a a t a a c t t t a c t t g c c g a g a a a a c

2460 1 5 10 MQFKVYTYKRES ccdB c t g a c g a g a a c a g g g g c t g g t g a a a t g c a g t t t a a g g t t t a c a c c t a t a a a a g a g a g a g c g a c t g c t c t t g t c c c c g a c c a c t t t a c g t c a a a t t c c a a a t g t g g a t a t t t t c t c t c t c g

TspMIXmaISmaISrfI 2520 15 20 25 30 RYRLFVDVQSDIIDTPGRRM ccdB c g t t a t c g t c t g t t t g t g g a t g t a c a g a g t g a t a t t a t t g a c a c g c c c g g g c g a c g g a t g g c a a t a g c a g a c a a a c a c c t a c a t g t c t c a c t a t a a t a a c t g t g c g g g c c c g c t g c c t a c

2580 35 40 45 50 VIPLASARLLSDKVPRELYP ccdB g t g a t c c c c c t g g c c a g t g c a c g t c t g c t g t c a g a t a a a g t c c c c c g t g a a c t t t a c c c g c a c t a g g g g g a c c g g t c a c g t g c a g a c g a c a g t c t a t t t c a g g g g g c a c t t g a a a t g g g c

2640 55 60 65 70 VVHIGDESWRMMTTDMASVP ccdB G C A T G A T G A C C A C C G A T A T G ccdB-fwd g t g g t g c a t a t c g g g g a t g a a a g c t g g c g c a t g a t g a c c a c c g a t a t g g c c a g t g t g c c g c a c c a c g t a t a g c c c c t a c t t t c g a c c g c g t a c t a c t g g t g g c t a t a c c g g t c a c a c g g c

2700 75 80 85 90 VSVIGEEVADLSHRENDIKN ccdB g t c t c c g t t a t c g g g g a a g a a g t g g c t g a t c t c a g c c a c c g c g a a a a t g a c a t c a a a a a c c a g a g g c a a t a g c c c c t t c t t c a c c g a c t a g a g t c g g t g g c g c t t t t a c t g t a g t t t t t g

2760 95 100 AINLMFWGI* ccdB g c c a t t a a c c t g a t g t t c t g g g g a a t a t a a a t g t c a g g c t c c c t t a t a c a c a g c c a g t c t c g g t a a t t g g a c t a c a a g a c c c c t t a t a t t t a c a g t c c g a g g g a a t a t g t g t c g g t c a g a

SalI 2820 attR2 g c a g g t c g a c c a t a g t g a c t g g a t a t g t t g t g t t t t a c a g t a t t a t g t a g t c t g t t t t t t c g t c c a g c t g g t a t c a c t g a c c t a t a c a a c a c a a a a t g t c a t a a t a c a t c a g a c a a a a a a

2880 attR2 a t g c a a a a t c t a a t t t a a t a t a t t g a t a t t t a t a t c a t t t t a c g t t t c t c g t t c a g c t t t t a c g t t t t a g a t t a a a t t a t a t a a c t a t a a a t a t a g t a a a a t g c a a a g a g c a a g t c g a a a

2940 attR2 MASEARKTKKKI c t t g t a c a a a g t g g t t g a t a a c a g c a t g g c t a g t g a a g c t c g a a a a a c a a a g a a a a a a a t g a a c a t g t t t c a c c a a c t a t t g t c g t a c c g a t c a c t t c g a g c t t t t t g t t t c t t t t t t t a

3000 KGIQQATAGVSQDTSENPNK c a a a g g g a t t c a g c a a g c c a c t g c a g g a g t c t c a c a a g a c a c t t c g g a a a a t c c t a a c a a g t t t c c c t a a g t c g t t c g g t g a c g t c c t c a g a g t g t t c t g t g a a g c c t t t t a g g a t t g t t

3060 TIVPAALPQLTPTLVSLLEV a a c a a t a g t t c c t g c a g c a t t a c c a c a g c t c a c c c c t a c c t t g g t g t c a c t g c t g g a g g t t t g t t a t c a a g g a c g t c g t a a t g g t g t c g a g t g g g g a t g g a a c c a c a g t g a c g a c c t c c a

3120 IEPEVLYAGYDSSVPDSAWR g a t t g a a c c c g a g g t g t t g t a t g c a g g a t a t g a t a g c t c t g t t c c a g a t t c a g c a t g g a g c t a a c t t g g g c t c c a c a a c a t a c g t c c t a t a c t a t c g a g a c a a g g t c t a a g t c g t a c c t c

3180 IMTTLNMLGGRQVIAAVKWA a a t t a t g a c c a c a c t c a a c a t g t t a g g t g g g c g t c a a g t g a t t g c a g c a g t g a a a t g g g c t t a a t a c t g g t g t g a g t t g t a c a a t c c a c c c g c a g t t c a c t a a c g t c g t c a c t t t a c c c g

3240 KAIPGFRNLHLDDQMTLLQY a a a g g c g a t a c c a g g c t t c a g a a a c t t a c a c c t g g a t g a c c a a a t g a c c c t g c t a c a g t a t t t c c g c t a t g g t c c g a a g t c t t t g a a t g t g g a c c t a c t g g t t t a c t g g g a c g a t g t c a t

3300 SWMFLMAFALGWRSYRQSSG c t c a t g g a t g t t t c t c a t g g c a t t t g c c c t g g g t t g g a g a t c a t a c a g a c a a t c a a g t g g g a g t a c c t a c a a a g a g t a c c g t a a a c g g g a c c c a a c c t c t a g t a t g t c t g t t a g t t c a c c

3360 NLLCFAPDLIINEQRMSLPC a a a c c t g c t c t g c t t t g c t c c t g a t c t g a t t a t t a a t g a g c a g a g a a t g t c t c t a c c c t g t t t g g a c g a g a c g a a a c g a g g a c t a g a c t a a t a a t t a c t c g t c t c t t a c a g a g a t g g g a c

3420 MYDQCKHMLFVSSELQRLQV c a t g t a t g a c c a a t g t a a a c a c a t g c t g t t t g t c t c c t c t g a a t t a c a a a g a t t g c a g g t g t a c a t a c t g g t t a c a t t t g t g t a c g a c a a a c a g a g g a g a c t t a a t g t t t c t a a c g t c c a

Bsu36I 3480 SYEEYLCMKTLLLLSSVPKE a t c c t a t g a a g a g t a t c t c t g t a t g a a a a c c t t a c t g c t t c t c t c c t c a g t t c c t a a g g a t a g g a t a c t t c t c a t a g a g a c a t a c t t t t g g a a t g a c g a a g a g a g g a g t c a a g g a t t c c t

BstBI 3540 GLKSQELFDEIRMTYIKELG a g g t c t g a a g a g c c a a g a g t t a t t t g a t g a g a t t c g a a t g a c t t a t a t c a a a g a g c t a g g t c c a g a c t t c t c g g t t c t c a a t a a a c t a c t c t a a g c t t a c t g a a t a t a g t t t c t c g a t c c

3600 KAIVKREGNSSQNWQRFYQL a a a a g c c a t c g t c a a a a g g g a a g g g a a c t c c a g t c a g a a c t g g c a a c g g t t t t a c c a a c t t t t t c g g t a g c a g t t t t c c c t t c c c t t g a g g t c a g t c t t g a c c g t t g c c a a a a t g g t t g a

3660 TKLLDSMHEVVENLLTYCFQ g a c a a a a c t t c t g g a c t c c a t g c a t g a g g t g g t t g a g a a t c t c c t t a c c t a c t g c t t c c a c t g t t t t g a a g a c c t g a g g t a c g t a c t c c a c c a a c t c t t a g a g g a a t g g a t g a c g a a g g t

3720 TFLDKTMSIEFPEMLAEIIT g a c a t t t t t g g a t a a g a c c a t g a g t a t t g a a t t c c c a g a g a t g t t a g c t g a a a t c a t c a c c t g t a a a a a c c t a t t c t g g t a c t c a t a a c t t a a g g g t c t c t a c a a t c g a c t t t a g t a g t g

3780 NQIPKYSNGNIKKLLFHQK* t a a t c a g a t a c c a a a a t a t t c a a a t g g a a a t a t c a a g a a g c t g c t g t t t c a t c a a a a a t a a t t a g t c t a t g g t t t t a t a a g t t t a c c t t t a t a g t t c t t c g a c g a c a a a g t a g t t t t t a t

AflIIEco53kIBanIISacI 3840 NOS terminator g a g c t c g a a t t t c c c c g a t c g t t c a a a c a t t t g g c a a t a a a g t t t c t t a a g a t t g a a t c c c t c g a g c t t a a a g g g g c t a g c a a g t t t g t a a a c c g t t a t t t c a a a g a a t t c t a a c t t a g g

3900 NOS terminator t g t t g c c g g t c t t g c g a t g a t t a t c a t a t a a t t t c t g t t g a a t t a c g t t a a g c a t g t a a t a c a a c g g c c a g a a c g c t a c t a a t a g t a t a t t a a a g a c a a c t t a a t g c a a t t c g t a c a t t a

3960 NOS terminator a a t t a a c a t g t a a t g c a t g a c g t t a t t t a t g a g a t g g g t t t t t a t g a t t a g a g t c c c g c a t t a a t t g t a c a t t a c g t a c t g c a a t a a a t a c t c t a c c c a a a a a t a c t a a t c t c a g g g c g t

4020 NOS terminator a t t a t a c a t t t a a t a c g c g a t a g a a a a c a a a a t a t a g c g c g c a a a c t a g g a t a a a t t a t c t a a t a t g t a a a t t a t g c g c t a t c t t t t g t t t t a t a t c g c g c g t t t g a t c c t a t t t a a t a g

MauBI 4080 NOS terminator g c g c g c g g t g t c a t c t a t g t t a c t a g a t c g g g a a t t g g t t c c g g a a c c a a t t c g t a a t c a c g c g c g c c a c a g t a g a t a c a a t g a t c t a g c c c t t a a c c a a g g c c t t g g t t a a g c a t t a g t

4140 -10 M13 rev lac operator lac promoter C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t

4200 -35 lac promoter CAP binding site g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a g g c c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t c c g

AscI 4260 t g a a t t a g g c g c g c c t a t t t c t g a a t t c a a c a c a t t g c g g a c g t t t t t a a t g t a c t g a a t a c t t a a t c c g c g c g g a t a a a g a c t t a a g t t g t g t a a c g c c t g c a a a a a t t a c a t g a c t t a

4320 CaMV poly(A) signal t a a c g c c g a a t t a a t t c g g g g g a t c t g g a t t t t a g t a c t g g a t t t t g g t t t t a g g a a t t a a t t g c g g c t t a a t t a a g c c c c c t a g a c c t a a a a t c a t g a c c t a a a a c c a a a a t c c t t a a t

4380 CaMV poly(A) signal g a a a t t t t a t t g a t a g a a g t a t t t t a c a a a t a c a a a t a c a t a c t a a g g g t t t c t t a t a t g c t t t a a a a t a a c t a t c t t c a t a a a a t g t t t a t g t t t a t g t a t g a t t c c c a a a g a a t a t a c

4440 CaMV poly(A) signal c t c a a c a c a t g a g c g a a a c c c t a t a g g a a c c c t a a t t c c c t t a t c t g g g a a c t a c t c a c a g a g t t g t g t a c t c g c t t t g g g a t a t c c t t g g g a t t a a g g g a a t a g a c c c t t g a t g a g t g t

4500 * CaMV poly(A) signal HygR * c a t t a t t a t g g a g a a a c t c g a g c t t g t c g a t c g a c a g a t c c c g g t c g g c a t c t a c t c t a t g t a a t a a t a c c t c t t t g a g c t c g a a c a g c t a g c t g t c t a g g g c c a g c c g t a g a t g a g a t a

4560 340 335 330 325 KKARPRTSPRRNGSDALVEV HygR KKARPRTSPRRNGSDALVEV t t c t t t g c c c t c g g a c g a g t g c t g g g g c g t c g g t t t c c a c t a t c g g c g a g t a c t t c t a c a a a g a a a c g g g a g c c t g c t c a c g a c c c c g c a g c c a a a g g t g a t a g c c g c t c a t g a a g a t g t

4620 320 315 310 305 CGDTWVAASRRAIQTRGVTG HygR CGDTWVAASRRAIQTRGVTG c a g c c a t c g g t c c a g a c g g c c g c g c t t c t g c g g g c g a t t t g t g t a c g c c c g a c a g t c c c g g t c g g t a g c c a g g t c t g c c g g c g c g a a g a c g c c c g c t a a a c a c a t g c g g g c t g t c a g g g c

4680 300 295 290 285 AGSRVIADCRGQAWAADDFN HygR AGSRVIADCRGQAWAADDFN g c t c c g g a t c g g a c g a t t g c g t c g c a t c g a c c c t g c g c c c a a g c t g c a t c a t c g a a a t t g c g a g g c c t a g c c t g c t a a c g c a g c g t a g c t g g g a c g c g g g t t c g a c g t a g t a g c t t t a a c

4740 280 275 270 265 GDVLSQYLQDLGIRLMYARL HygR GDVLSQYLQDLGIRLMYARL c c g t c a a c c a a g c t c t g a t a g a g t t g g t c a a g a c c a a t g c g g a g c a t a t a c g c c c g g a g t g g c a g t t g g t t c g a g a c t a t c t c a a c c a g t t c t g g t t a c g c c t c g t a t a t g c g g g c c t c a

4800 260 255 250 245 RPSGALEPHRREFYRTQQEM HygR RPSGALEPHRREFYRTQQEM c g t g g c g a t c c t g c a a g c t c c g g a t g c c t c c g c t c g a a g t a g c g c g t c t g c t g c t c c a t a g c a c c g c t a g g a c g t t c g a g g c c t a c g g a g g c g a g c t t c a t c g c g c a g a c g a c g a g g t a t

4860 240 235 230 225 CALWPRWFFINAVEYQSDGF HygR CALWPRWFFINAVEYQSDGF c a a g c c a a c c a c g g c c t c c a g a a g a a g a t g t t g g c g a c c t c g t a t t g g g a a t c c c c g a a c g t t c g g t t g g t g c c g g a g g t c t t c t t c t a c a a c c g c t g g a g c a t a a c c c t t a g g g g c t t g

4920 220 215 210 205 MAESWDIVATIRGNDTLVNN HygR MAESWDIVATIRGNDTLVNN a t c g c c t c g c t c c a g t c a a t g a c c g c t g t t a t g c g g c c a t t g t c c g t c a g g a c a t t g t t g t a g c g g a g c g a g g t c a g t t a c t g g c g a c a a t a c g c c g g t a a c a g g c a g t c c t g t a a c a a c

4980 200 195 190 185 SGFDAHVLHRVEPCDEAWLM HygR SGFDAHVLHRVEPCDEAWLM g a g c c g a a a t c c g c g t g c a c g a g g t g c c g g a c t t c g g g g c a g t c c t c g g c c c a a a g c a t c c t c g g c t t t a g g c g c a c g t g c t c c a c g g c c t g a a g c c c c g t c a g g a g c c g g g t t t c g t a g

5040 180 175 170 165 LEDLAQAVSASVTDDMVTQW HygR LEDLAQAVSASVTDDMVTQW a g c t c a t c g a g a g c c t g c g c g a c g g a c g c a c t g a c g g t g t c g t c c a t c a c a g t t t g c c a g t c g a g t a g c t c t c g g a c g c g c t g c c t g c g t g a c t g c c a c a g c a g g t a g t g t c a a a c g g t c

5100 160 155 150 145 HYVHPDAIACIFDRWTTYQG HygR HYVHPDAIACIFDRWTTYQG t g a t a c a c a t g g g g a t c a g c a a t c g c g c a t a t g a a a t c a c g c c a t g t a g t g t a t t g a c c g a c t a t g t g t a c c c c t a g t c g t t a g c g c g t a t a c t t t a g t g c g g t a c a t c a c a t a a c t g g c

AsiSIRsrII 5160 140 135 130 125 IGQPGFPGFGSTQSLDAAAI HygR IGQPGFPGFGSTQSLDAAAI a t t c c t t g c g g t c c g a a t g g g c c g a a c c c g c t c g t c t g g c t a a g a t c g g c c g c a g c g a t c t a a g g a a c g c c a g g c t t a c c c g g c t t g g g c g a g c a g a c c g a t t c t a g c c g g c g t c g c t a g

5220 120 115 110 105 ADMAEAVPQLVAPLETEPLD HygR ADMAEAVPQLVAPLETEPLD g c a t c c a t a g c c t c c g c g a c c g g t t g t a g a a c a g c g g g c a g t t c g g t t t c a g g c a g g t c t c g t a g g t a t c g g a g g c g c t g g c c a a c a t c t t g t c g c c c g t c a a g c c a a a g t c c g t c c a g a

5280 100 95 90 85 QLTVGQSRRSICYTLSESFE HygR QLTVGQSRRSICYTLSESFE t g c a a c g t g a c a c c c t g t g a a c g g c g g g a g a t g c a a t a g g t c a g g c t c t c g c t a a a c t c c a c g t t g c a c t g t g g g a c a c t t g c c g c c c t c t a c g t t a t c c a g t c c g a g a g c g a t t t g a g g

5340 80 75 70 65 GIDLVEPIPLAASAFHRYVY HygR GIDLVEPIPLAASAFHRYVY c c a a t g t c a a g c a c t t c c g g a a t c g g g a g c g c g g c c g a t g c a a a g t g c c g a t a a a c a t a a g g t t a c a g t t c g t g a a g g c c t t a g c c c t c g c g c c g g c t a c g t t t c a c g g c t a t t t g t a t t

5400 60 55 50 45 RDKYFGDACSNVRLVYGRGG HygR RDKYFGDACSNVRLVYGRGG c g a t c t t t g t a g a a a c c a t c g g c g c a g c t a t t t a c c c g c a g g a c a t a t c c a c g c c c t c c t g c t a g a a a c a t c t t t g g t a g c c g c g t c g a t a a a t g g g c g t c c t g t a t a g g t g c g g g a g g a

5460 40 35 30 25 VDFSFARSEEGESLQMLDSV HygR VDFSFARSEEGESLQMLDSV a c a t c g a a g c t g a a a g c a c g a g a t t c t t c g c c c t c c g a g a g c t g c a t c a g g t c g g a g a c g t g t a g c t t c g a c t t t c g t g c t c t a a g a a g c g g g a g g c t c t c g a c g t a g t c c a g c c t c t g c

ZraIAatII 5520 20 15 10 5 SDFKEILFKEVSTATLEPKK HygR SDFKEILFKEVSTATLEPKK c t g t c g a a c t t t t c g a t c a g a a a c t t c t c g a c a g a c g t c g c g g t g a g t t c a g g c t t t t t c g a c a g c t t g a a a a g c t a g t c t t t g a a g a g c t g t c t g c a g c g c c a c t c a a g t c c g a a a a a g

5580 1 M HygR M a t a t c t c a t t g c c c c c c g g a t c t g c g a a a g c t c g a g a g a g a t a g a t t t g t a g a g a g a g a c t a t a g a g t a a c g g g g g g c c t a g a c g c t t t c g a g c t c t c t c t a t c t a a a c a t c t c t c t c t g

5640 CaMV 35S promoter (enhanced) t g g t g a t t t c a g c g t g t c c t c t c c a a a t g a a a t g a a c t t c c t t a t a t a g a g g a a g g t c t t a c c a c t a a a g t c g c a c a g g a g a g g t t t a c t t t a c t t g a a g g a a t a t a t c t c c t t c c a g a a

5700 CaMV 35S promoter (enhanced) g c g a a g g a t a g t g g g a t t g t g c g t c a t c c c t t a c g t c a g t g g a g a t a t c a c a t c a a t c c a c g c t t c c t a t c a c c c t a a c a c g c a g t a g g g a a t g c a g t c a c c t c t a t a g t g t a g t t a g g t

5760 CaMV 35S promoter (enhanced) c t t g c t t t g a a g a c g t g g t t g g a a c g t c t t c t t t t t c c a c g a t g c t c c t c g t g g g t g g g g g a a c g a a a c t t c t g c a c c a a c c t t g c a g a a g a a a a a g g t g c t a c g a g g a g c a c c c a c c c c

5820 CaMV 35S promoter (enhanced) g t c c a t c t t t g g g a c c a c t g t c g g c a g a g g c a t c t t g a a c g a t a g c c t t t c c t t t a t c g c c a g g t a g a a a c c c t g g t g a c a g c c g t c t c c g t a g a a c t t g c t a t c g g a a a g g a a a t a g c g

5880 CaMV 35S promoter (enhanced) a a t g a t g g c a t t t g t a g g t g c c a c c t t c c t t t t c t a c t g t c c t t t t g a t g a a g t g a c a g a t t a c t a c c g t a a a c a t c c a c g g t g g a a g g a a a a g a t g a c a g g a a a a c t a c t t c a c t g t c t

5940 CaMV 35S promoter (enhanced) t a g c t g g g c a a t g g a a t c c g a g g a g g t t t c c c g a t a t t a c c c t t t g t t g a a a a g t c t c a a a t c g a c c c g t t a c c t t a g g c t c c t c c a a a g g g c t a t a a t g g g a a a c a a c t t t t c a g a g t t

6000 CaMV 35S promoter (enhanced) t a g c c c t t t g g t c t t c t g a g a c t g t a t c t t t g a t a t t c t t g g a g t a g a c g a g a g t g t c g t a t c g g g a a a c c a g a a g a c t c t g a c a t a g a a a c t a t a a g a a c c t c a t c t g c t c t c a c a g c a

6060 CaMV 35S promoter (enhanced) g c t c c a c c a t g t t c a c a t c a a t c c a c t t g c t t t g a a g a c g t g g t t g g a a c g t c t t c t t t t c g a g g t g g t a c a a g t g t a g t t a g g t g a a c g a a a c t t c t g c a c c a a c c t t g c a g a a g a a a a

6120 CaMV 35S promoter (enhanced) t c c a c g a t g c t c c t c g t g g g t g g g g g t c c a t c t t t g g g a c c a c t g t c g g c a g a g g c a t c t a g g t g c t a c g a g g a g c a c c c a c c c c c a g g t a g a a a c c c t g g t g a c a g c c g t c t c c g t a g a

6180 CaMV 35S promoter (enhanced) t g a a c g a t a g c c t t t c c t t t a t c g c a a t g a t g g c a t t t g t a g g t g c c a c c t t c c t t t t c t a c t t g c t a t c g g a a a g g a a a t a g c g t t a c t a c c g t a a a c a t c c a c g g t g g a a g g a a a a g a

6240 CaMV 35S promoter (enhanced) a c t g t c c t t t t g a t g a a g t g a c a g a t a g c t g g g c a a t g g a a t c c g a g g a g g t t t c c c g a t t g a c a g g a a a a c t a c t t c a c t g t c t a t c g a c c c g t t a c c t t a g g c t c c t c c a a a g g g c t a

6300 CaMV 35S promoter (enhanced) a t t a c c c t t t g t t g a a a a g t c t c a a t a g c c c t t t g g t c t t c t g a g a c t g t a t c t t t g a t a t a a t g g g a a a c a a c t t t t c a g a g t t a t c g g g a a a c c a g a a g a c t c t g a c a t a g a a a c t a t

6360 t t c t t g g a g t a g a c g a g a g t g t c g t g c t c c a c c a t g t t g g c a a g c t g c t c t a g c c a a t a c a a g a a c c t c a t c t g c t c t c a c a g c a c g a g g t g g t a c a a c c g t t c g a c g a g a t c g g t t a t g

6420 g c a a a c c g a a t t c a g a a a t a a a t t c a g c c t a a t t c g g c g t t a a t t c a g t a c a t t a a a a a c c g t t t g g c t t a a g t c t t t a t t t a a g t c g g a t t a a g c c g c a a t t a a g t c a t g t a a t t t t t g

6480 LB T-DNA repeat g t c c g c a a t g t g t t a t t a a g t t g t c t a a g c g t c a a t t t g t t t a c a c c a c a a t a t a t c c t g c a g g c g t t a c a c a a t a a t t c a a c a g a t t c g c a g t t a a a c a a a t g t g g t g t t a t a t a g g a c

6540 LB T-DNA repeat c c a c c a g c c a g c c a a c a g c t c c c c g a c c g g c a g c t c g g c a c a a a a t c a c c a c t c g a t a c a g g t g g t c g g t c g g t t g t c g a g g g g c t g g c c g t c g a g c c g t g t t t t a g t g g t g a g c t a t g t

6600 g g c a g c c c a t c a g t c c g g g a c g g c g t c a g c g g g a g a g c c g t t g t a a g g c g g c a g a c t t t g c c g t c g g g t a g t c a g g c c c t g c c g c a g t c g c c c t c t c g g c a a c a t t c c g c c g t c t g a a a c

6660 c t c a t g t t a c c g a t g c t a t t c g g a a g a a c g g c a a c t a a g c t g c c g g g t t t g a a a c a c g g a g a g t a c a a t g g c t a c g a t a a g c c t t c t t g c c g t t g a t t c g a c g g c c c a a a c t t t g t g c c t

6720 t g a t c t c g c g g a g g g t a g c a t g t t g a t t g t a a c g a t g a c a g a g c g t t g c t g c c t g t g a t c a c t a g a g c g c c t c c c a t c g t a c a a c t a a c a t t g c t a c t g t c t c g c a a c g a c g g a c a c t a g

6780 a a t t c g g g c a c g a a c c c a g t g g a c a t a a g c c t g t t c g g t t c g t a a g c t g t a a t g c a a g t a t t a a g c c c g t g c t t g g g t c a c c t g t a t t c g g a c a a g c c a a g c a t t c g a c a t t a c g t t c a t

6840 MRSRNWSRTLTERSGGNGA g c g t a t g c g c t c a c g c a a c t g g t c c a g a a c c t t g a c c g a a c g c a g c g g t g g t a a c g g c g c c g c a t a c g c g a g t g c g t t g a c c a g g t c t t g g a a c t g g c t t g c g t c g c c a c c a t t g c c g c g

6900 VAVFMACYDCFFGVQSMPRA a g t g g c g g t t t t c a t g g c t t g t t a t g a c t g t t t t t t t g g g g t a c a g t c t a t g c c t c g g g c t c a c c g c c a a a a g t a c c g a a c a a t a c t g a c a a a a a a a c c c c a t g t c a g a t a c g g a g c c c g

6960 SKQQARYAVGRCLMLWSSND a t c c a a g c a g c a a g c g c g t t a c g c c g t g g g t c g a t g t t t g a t g t t a t g g a g c a g c a a c g a t a g g t t c g t c g t t c g c g c a a t g c g g c a c c c a g c t a c a a a c t a c a a t a c c t c g t c g t t g c t

7020 1 5 MGEAVI SmR VTQQGSRPKTKLNIMGEAVI t g t t a c g c a g c a g g g c a g t c g c c c t a a a a c a a a g t t a a a c a t c a t g g g g g a a g c g g t g a t a c a a t g c g t c g t c c c g t c a g c g g g a t t t t g t t t c a a t t t g t a g t a c c c c c t t c g c c a c t a

7080 10 15 20 25 AEVSTQLSEVVGVIERHLEP SmR AEVSTQLSEVVGVIERHLEP c g c c g a a g t a t c g a c t c a a c t a t c a g a g g t a g t t g g c g t c a t c g a g c g c c a t c t c g a a c c g c g g c t t c a t a g c t g a g t t g a t a g t c t c c a t c a a c c g c a g t a g c t c g c g g t a g a g c t t g g

7140 30 35 40 45 TLLAVHLYGSAVDGGLKPHS SmR TLLAVHLYGSAVDGGLKPHS g a c g t t g c t g g c c g t a c a t t t g t a c g g c t c c g c a g t g g a t g g c g g c c t g a a g c c a c a c a g c t g c a a c g a c c g g c a t g t a a a c a t g c c g a g g c g t c a c c t a c c g c c g g a c t t c g g t g t g t c

BstEII 7200 50 55 60 65 DIDLLVTVTVRLDETTRRAL SmR DIDLLVTVTVRLDETTRRAL t g a t a t t g a t t t g c t g g t t a c g g t g a c c g t a a g g c t t g a t g a a a c a a c g c g g c g a g c t t t a c t a t a a c t a a a c g a c c a a t g c c a c t g g c a t t c c g a a c t a c t t t g t t g c g c c g c t c g a a a

7260 70 75 80 85 INDLLETSASPGESEILRAV SmR INDLLETSASPGESEILRAV g a t c a a c g a c c t t t t g g a a a c t t c g g c t t c c c c t g g a g a g a g c g a g a t t c t c c g c g c t g t c t a g t t g c t g g a a a a c c t t t g a a g c c g a a g g g g a c c t c t c t c g c t c t a a g a g g c g c g a c a

BlpI 7320 90 95 100 105 EVTIVVHDDIIPWRYPAKRE SmR EVTIVVHDDIIPWRYPAKRE a g a a g t c a c c a t t g t t g t g c a c g a c g a c a t c a t t c c g t g g c g t t a t c c a g c t a a g c g c g a t c t t c a g t g g t a a c a a c a c g t g c t g c t g t a g t a a g g c a c c g c a a t a g g t c g a t t c g c g c t

7380 110 115 120 125 LQFGEWQRNDILAGIFEPAT SmR LQFGEWQRNDILAGIFEPAT a c t g c a a t t t g g a g a a t g g c a g c g c a a t g a c a t t c t t g c a g g t a t c t t c g a g c c a g c c a c t g a c g t t a a a c c t c t t a c c g t c g c g t t a c t g t a a g a a c g t c c a t a g a a g c t c g g t c g g t g

7440 130 135 140 145 IDIDLAILLTKAREHSVALV SmR IDIDLAILLTKAREHSVALV g a t c g a c a t t g a t c t g g c t a t c t t g c t g a c a a a a g c a a g a g a a c a t a g c g t t g c c t t g g t c t a g c t g t a a c t a g a c c g a t a g a a c g a c t g t t t t c g t t c t c t t g t a t c g c a a c g g a a c c a

7500 150 155 160 165 GPAAEELFDPVPEQDLFEAL SmR GPAAEELFDPVPEQDLFEAL a g g t c c a g c g g c g g a g g a a c t c t t t g a t c c g g t t c c t g a a c a g g a t c t a t t t g a g g c g c t t c c a g g t c g c c g c c t c c t t g a g a a a c t a g g c c a a g g a c t t g t c c t a g a t a a a c t c c g c g a

7560 170 175 180 185 NETLTLWNSPPDWAGDERNV SmR NETLTLWNSPPDWAGDERNV a a a t g a a a c c t t a a c g c t a t g g a a c t c g c c g c c c g a c t g g g c t g g c g a t g a g c g a a a t g t t t t a c t t t g g a a t t g c g a t a c c t t g a g c g g c g g g c t g a c c c g a c c g c t a c t c g c t t t a c a

7620 190 195 200 205 VLTLSRIWYSAVTGKIAPKD SmR VLTLSRIWYSAVTGKIAPKD a g t g c t t a c g t t g t c c c g c a t t t g g t a c a g c g c a g t a a c c g g c a a a a t c g c g c c g a a g g a t c a c g a a t g c a a c a g g g c g t a a a c c a t g t c g c g t c a t t g g c c g t t t t a g c g c g g c t t c c t

7680 210 215 220 225 VAADWAMERLPAQYQPVILE SmR VAADWAMERLPAQYQPVILE t g t c g c t g c c g a c t g g g c a a t g g a g c g c c t g c c g g c c c a g t a t c a g c c c g t c a t a c t t g a a c a g c g a c g g c t g a c c c g t t a c c t c g c g g a c g g c c g g g t c a t a g t c g g g c a g t a t g a a c t

7740 230 235 240 245 ARQAYLGQEEDRLASRADQL SmR ARQAYLGQEEDRLASRADQL a g c t a g a c a g g c t t a t c t t g g a c a a g a a g a a g a t c g c t t g g c c t c g c g c g c a g a t c a g t t t c g a t c t g t c c g a a t a g a a c c t g t t c t t c t t c t a g c g a a c c g g a g c g c g c g t c t a g t c a a

7800 250 255 260 EEFVHYVKGEITKVVGK* SmR EEFVHYVKGEITKVVGK* g g a a g a a t t t g t c c a c t a c g t g a a a g g c g a g a t c a c c a a g g t a g t c g g c a a a t a a t g t c t c c t t c t t a a a c a g g t g a t g c a c t t t c c g c t c t a g t g g t t c c a t c a g c c g t t t a t t a c a g a

7860 a g c t a g a a a t t c g t t c a a g c c g a c g c c g c t t c g c g g c g c g g c t t a a c t c a a g c g t t a g a t t c g a t c t t t a a g c a a g t t c g g c t g c g g c g a a g c g c c g c g c c g a a t t g a g t t c g c a a t c t a

7920 g c a c t a a g c a c a t a a t t g c t c a c a g c c a a a c t a t c a g g t c a a g t c t g c t t t t a t t a t t t t c g t g a t t c g t g t a t t a a c g a g t g t c g g t t t g a t a g t c c a g t t c a g a c g a a a a t a a t a a a a

7980 t a a g c g t g c a t a a t a a g c c c t a c a c a a a t t g g g a g a t a t a t c a t g c a t g a c c a a a a t c c c a t t c g c a c g t a t t a t t c g g g a t g t g t t t a a c c c t c t a t a t a g t a c g t a c t g g t t t t a g g g

8040 t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g

8100 ori t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g

8160 ori a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a

8220 ori c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a

8280 ori c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g

8340 ori t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t

8400 ori g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g

8460 ori c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c

8520 ori g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t

8580 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a

8640 ori t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t

8700 c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g

8760 g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c

8820 bom A G C G A G T C A G T G A G C G A G L4440 c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c t g a t g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g a c t a

8880 bom g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a t g g t g c a c t c t c a g c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t a c c a c g t g a g a g t c

8940 bom A T G T T A G A C G A G A C T A C G G C pRS-marker t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t a c a c t c c g c t a t c g c t a c g t g a c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c a t a t g t g a g g c g a t a g c g a t g c a c t g

9000 bom t g g g t c a t g g c t g c g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t a c c c a g t a c c g a c g c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a

9060 C C G G G A G C T G C A T G T G T C A G pGEX 3' c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t c a g g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a g t c

KasINarISfoISgrAIMreIPluTI 9120 A G G pGEX 3' a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g g c a g g g t g c c t t g a t g t g g g c g c c g g c g t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c c g t c c c a c g g a a c t a c a c c c g c g g c c g c

9180 g t c g a g t g g c g a c g g c g c g g c t t g t c c g c g c c c t g g t a g a t t g c c t g g c c g t a g g c c a g c c a g c t c a c c g c t g c c g c g c c g a a c a g g c g c g g g a c c a t c t a a c g g a c c g g c a t c c g g t c g

9240 c a t t t t t g a g c g g c c a g c g g c c g c g a t a g g c c g a c g c g a a g c g g c g g g g c g t a g g g a g c g g t a a a a a c t c g c c g g t c g c c g g c g c t a t c c g g c t g c g c t t c g c c g c c c c g c a t c c c t c g c

9300 pVS1 oriV c a g c g a c c g a a g g g t a g g c g c t t t t t g c a g c t c t t c g g c t g t g c g c t g g c c a g a c a g t t a g t c g c t g g c t t c c c a t c c g c g a a a a a c g t c g a g a a g c c g a c a c g c g a c c g g t c t g t c a a t

9360 pVS1 oriV t g c a c a g g c c a g g c g g g t t t t a a g a g t t t t a a t a a g t t t t a a a g a g t t t t a g g c g g a a a a a c g t g t c c g g t c c g c c c a a a a t t c t c a a a a t t a t t c a a a a t t t c t c a a a a t c c g c c t t t t

9420 pVS1 oriV a t c g c c t t t t t t c t c t t t t a t a t c a g t c a c t t a c a t g t g t g a c c g g t t c c c a a t g t a c g g t a g c g g a a a a a a g a g a a a a t a t a g t c a g t g a a t g t a c a c a c t g g c c a a g g g t t a c a t g c c

9480 pVS1 oriV c t t t g g g t t c c c a a t g t a c g g g t t c c g g t t c c c a a t g t a c g g c t t t g g g t t c c c a a t g t a g a a a c c c a a g g g t t a c a t g c c c a a g g c c a a g g g t t a c a t g c c g a a a c c c a a g g g t t a c a t

9540 pVS1 oriV c g t g c t a t c c a c a g g a a a g a g a c c t t t t c g a c c t t t t t c c c c t g c t a g g g c a a t t t g c c c g c a c g a t a g g t g t c c t t t c t c t g g a a a a g c t g g a a a a a g g g g a c g a t c c c g t t a a a c g g g

9600 355 350 345 *SGASAEGEILNRY pVS1 RepA *SGASAEGEILNRY t a g c a t c t g c t c c g t a c a t t a g g a a c c g g c g g a t g c t t c g c c c t c g a t c a g g t t g c g g t a a t c g t a g a c g a g g c a t g t a a t c c t t g g c c g c c t a c g a a g c g g g a g c t a g t c c a a c g c c a t

9660 340 335 330 325 RMVLIPGAQGAEEKLDYEPL pVS1 RepA RMVLIPGAQGAEEKLDYEPL g c g c a t g a c t a g g a t c g g g c c a g c c t g c c c c g c c t c c t c c t t c a a a t c g t a c t c c g g c a g c g c g t a c t g a t c c t a g c c c g g t c g g a c g g g g c g g a g g a g g a a g t t t a g c a t g a g g c c g t c

9720 320 315 310 305 DNSGILKRVTFCFKKFEGAS pVS1 RepA DNSGILKRVTFCFKKFEGAS g t c a t t t g a c c c g a t c a g c t t g c g c a c g g t g a a a c a g a a c t t c t t g a a c t c t c c g g c g c t c a g t a a a c t g g g c t a g t c g a a c g c g t g c c a c t t t g t c t t g a a g a a c t t g a g a g g c c g c g a

9780 300 295 290 285 GSREYITKFLWRAEAKGAAR pVS1 RepA GSREYITKFLWRAEAKGAAR g c c a c t g c g t t c g t a g a t c g t c t t g a a c a a c c a t c t g g c t t c t g c c t t g c c t g c g g c g c g c g g t g a c g c a a g c a t c t a g c a g a a c t t g t t g g t a g a c c g a a g a c g g a a c g g a c g c c g c g c

BspDI*ClaI* 9840 280 275 270 265 RALRYLFRGIGPDILFYDPH pVS1 RepA RALRYLFRGIGPDILFYDPH g c g t g c c a g g c g g t a g a g a a a a c g g c c g a t g c c g g g a t c g a t c a a a a a g t a a t c g g g g t g c g c a c g g t c c g c c a t c t c t t t t g c c g g c t a c g g c c c t a g c t a g t t t t t c a t t a g c c c c a c

BmtINheI 9900 260 255 250 245 VTLVDPNKGETIERYMWDAL pVS1 RepA VTLVDPNKGETIERYMWDAL a a c c g t c a g c a c g t c c g g g t t c t t g c c t t c t g t g a t c t c g c g g t a c a t c c a a t c a g c t a g t t g g c a g t c g t g c a g g c c c a a g a a c g g a a g a c a c t a g a g c g c c a t g t a g g t t a g t c g a t c

9960 240 235 230 225 EIEIYEPRGTESKVIKYRSI pVS1 RepA EIEIYEPRGTESKVIKYRSI c t c g a t c t c g a t g t a c t c c g g c c g c c c g g t t t c g c t c t t t a c g a t c t t g t a g c g g c t a a t g a g c t a g a g c t a c a t g a g g c c g g c g g g c c a a a g c g a g a a a t g c t a g a a c a t c g c c g a t t a

BsiWI 10,020 220 215 210 205 LAEGESVTVLRGNKAKKTRQ pVS1 RepA LAEGESVTVLRGNKAKKTRQ c a a g g c t t c a c c c t c g g a t a c c g t c a c c a g g c g g c c g t t c t t g g c c t t c t t c g t a c g c t g g t t c c g a a g t g g g a g c c t a t g g c a g t g g t c c g c c g g c a a g a a c c g g a a g a a g c a t g c g a c

10,080 200 195 190 185 MAVHTTNLRICTEVLDDKQK pVS1 RepA MAVHTTNLRICTEVLDDKQK c a t g g c a a c g t g c g t g g t g t t t a a c c g a a t g c a g g t t t c t a c c a g g t c g t c t t t c t g c t t g t a c c g t t g c a c g c a c c a c a a a t t g g c t t a c g t c c a a a g a t g g t c c a g c a g a a a g a c g a a

10,140 180 175 170 165 GGDARRCFKLVDAVHPRFVR pVS1 RepA GGDARRCFKLVDAVHPRFVR t c c g c c a t c g g c t c g c c g g c a g a a c t t g a g t a c g t c c g c a a c g t g t g g a c g g a a c a c g c g a g g c g g t a g c c g a g c g g c c g t c t t g a a c t c a t g c a g g c g t t g c a c a c c t g c c t t g t g c g c

10,200 160 155 150 145 GPKDGKGERYRNMSETLHSV pVS1 RepA MGN GPKDGKGERYRNMSETLHSV g c c g g g c t t g t c t c c c t t c c c t t c c c g g t a t c g g t t c a t g g a t t c g g t t a g a t g g g a a a c c g g c c c g a a c a g a g g g a a g g g a a g g g c c a t a g c c a a g t a c c t a a g c c a a t c t a c c c t t t g

10,260 140 135 130 125 AMLVLDYDWVSAMGAPGASV pVS1 RepA RHQYQVVIPHTGHAGRPCGN AMLVLDYDWVSAMGAPGASV c g c c a t c a g t a c c a g g t c g t a a t c c c a c a c a c t g g c c a t g c c g g c c g g c c c t g c g g a a a c g c g g t a g t c a t g g t c c a g c a t t a g g g t g t g t g a c c g g t a c g g c c g g c c g g g a c g c c t t t g

10,320 120 115 110 105 EVHGDPLEYRIVEGARRDRK pVS1 RepA LYVPVWKLVADHLASSSVTL EVHGDPLEYRIVEGARRDRK c t c t a c g t g c c c g t c t g g a a g c t c g t a g c g g a t c a c c t c g c c a g c t c g t c g g t c a c g c t t g a g a t g c a c g g g c a g a c c t t c g a g c a t c g c c t a g t g g a g c g g t c g a g c a g c c a g t g c g a a

10,380 100 95 90 85 SLRFVAVDMISRSDRTGVDY pVS1 RepA RQTENGHVHDAATIAGAHVI SLRFVAVDMISRSDRTGVDY c g a c a g a c g g a a a a c g g c c a c g t c c a t g a t g c t g c g a c t a t c g c g g g t g c c c a c g t c a t a g c t g t c t g c c t t t t g c c g g t g c a g g t a c t a c g a c g c t g a t a g c g c c c a c g g g t g c a g t a t

10,440 80 75 70 65 LMPVFFDPQEDGKPPKRISP pVS1 RepA EHRNEKIWLLVALGRLPNRR LMPVFFDPQEDGKPPKRISP g a g c a t c g g a a c g a a a a a a t c t g g t t g c t c g t c g c c c t t g g g c g g c t t c c t a a t c g a c g g c t c g t a g c c t t g c t t t t t t a g a c c a a c g a g c a g c g g g a a c c c g c c g a a g g a t t a g c t g c c

10,500 60 55 50 45 AGAAPPQRSEKRIRDAAAQW pVS1 RepA RTGCRRLPGFFADSISGRLP AGAAPPQRSEKRIRDAAAQW c g c a c c g g c t g c c g g c g g t t g c c g g g a t t c t t t g c g g a t t c g a t c a g c g g c c g c t t g c c a g c g t g g c c g a c g g c c g c c a a c g g c c c t a a g a a a c g c c t a a g c t a g t c g c c g g c g a a c g g t

10,560 40 35 30 25 SEGPRAEAEIRQRQAAQAAK pVS1 RepA RFTGACFCLDALPLGGLRGL SEGPRAEAEIRQRQAAQAAK c g a t t c a c c g g g g c g t g c t t c t g c c t c g a t g c g t t g c c g c t g g g c g g c c t g c g c g g c c t t g c t a a g t g g c c c c g c a c g a a g a c g g a g c t a c g c a a c g g c g a c c c g c c g g a c g c g c c g g a a

10,620 20 15 10 5 LKEVLDDGLAAGIQVPGSPK pVS1 RepA QLLHQVITQRRADLYRAGWF LKEVLDDGLAAGIQVPGSPK c a a c t t c t c c a c c a g g t c a t c a c c c a g c g c c g c g c c g a t t t g t a c c g g g c c g g a t g g t t t g t t g a a g a g g t g g t c c a g t a g t g g g t c g c g g c g c g g c t a a a c a t g g c c c g g c c t a c c a a a

10,680 1 RGSM pVS1 RepA ATAHADSSGLGVPVPLQGRQ RGSVGIGRAQPNWHWQLAPL g c g a c c g c t c a c g c c g a t t c c t c g g g c t t g g g g g t t c c a g t g c c a t t g c a g g g c c g g c a g c g c t g g c g a g t g c g g c t a a g g a g c c c g a a c c c c c a a g g t c a c g g t a a c g t c c c g g c c g t c

10,740 TTQPLTPGQPPVPPHMGHST CGLRKRRALRGNRWVHPM a c a a c c c a g c c g c t t a c g c c t g g c c a a c c g c c c g t t c c t c c a c a c a t g g g g c a t t c c a c g t g t t g g g t c g g c g a a t g c g g a c c g g t t g g c g g g c a a g g a g g t g t g t a c c c c g t a a g g t g c

10,800 ASVPGCS* g c g t c g g t g c c t g g t t g t t c t t g a t t t t c c a t g c c g c c t c c t t t a g c c g c t a a a a t t c a t c g c a g c c a c g g a c c a a c a a g a a c t a a a a g g t a c g g c g g a g g a a a t c g g c g a t t t t a a g t a

10,860 c t a c t c a t t t a t t c a t t t g c t c a t t t a c t c t g g t a g c t g c g c g a t g t a t t c a g a t a g c a g g a t g a g t a a a t a a g t a a a c g a g t a a a t g a g a c c a t c g a c g c g c t a c a t a a g t c t a t c g t c

10,920 c t c g g t a a t g g t c t t g c c t t g g c g t a c c g c g t a c a t c t t c a g c t t g g t g t g a t c c t c c g c g a g c c a t t a c c a g a a c g g a a c c g c a t g g c g c a t g t a g a a g t c g a a c c a c a c t a g g a g g c g

10,980 c g g c a a c t g a a a g t t g a c c c g c t t c a t g g c t g g c g t g t c t g c c a g g c t g g c c a a c g t t g c g c c g t t g a c t t t c a a c t g g g c g a a g t a c c g a c c g c a c a g a c g g t c c g a c c g g t t g c a a c g

11,040 a g c c t t g c t g c t g c g t g c g c t c g g a c g g c c g g c a c t t a g c g t g t t t g t g c t t t t g c t c a t t c g g a a c g a c g a c g c a c g c g a g c c t g c c g g c c g t g a a t c g c a c a a a c a c g a a a a c g a g t a

11,100 210 205 200 *ILKSKIEAALAQ pVS1 StaA *ILKSKIEAALAQ t t t c t c t t t a c c t c a t t a a c t c a a a t g a g t t t t g a t t t a a t t t c a g c g g c c a g c g c c t g g a a a g a g a a a t g g a g t a a t t g a g t t t a c t c a a a a c t a a a t t a a a g t c g c c g g t c g c g g a c c

11,160 195 190 185 180 VERAADGEPESELVTTGAAA pVS1 StaA VERAADGEPESELVTTGAAA a c c t c g c g g g c a g c g t c g c c c t c g g g t t c t g a t t c a a g a a c g g t t g t g c c g g c g g c g g c a t g g a g c g c c c g t c g c a g c g g g a g c c c a a g a c t a a g t t c t t g c c a a c a c g g c c g c c g c c g t

11,220 175 170 165 160 TGPYSVRQTIRSELIPLEYG pVS1 StaA TGPYSVRQTIRSELIPLEYG g t g c c t g g g t a g c t c a c g c g c t g c g t g a t a c g g g a c t c a a g a a t g g g c a g c t c g t a c c c g c a c g g a c c c a t c g a g t g c g c g a c g c a c t a t g c c c t g a g t t c t t a c c c g t c g a g c a t g g g c

11,280 155 150 145 140 ALAEAVEGGIRTGKIARSVV pVS1 StaA ALAEAVEGGIRTGKIARSVV g c c a g c g c c t c g g c a a c c t c a c c g c c g a t g c g c g t g c c t t t g a t c g c c c g c g a c a c g a c a c g g t c g c g g a g c c g t t g g a g t g g c g g c t a c g c g c a c g g a a a c t a g c g g g c g c t g t g c t g t

11,340 135 130 125 120 FAAQLRGDTVEIRQKVLEVL pVS1 StaA FAAQLRGDTVEIRQKVLEVL a a g g c c g c t t g t a g c c t t c c a t c c g t g a c c t c a a t g c g c t g c t t a a c c a g c t c c a c c a g g t t c c g g c g a a c a t c g g a a g g t a g g c a c t g g a g t t a c g c g a c g a a t t g g t c g a g g t g g t c c

11,400 115 110 105 100 DATAWIDYPSPQVPILVFDA pVS1 StaA DATAWIDYPSPQVPILVFDA t c g g c g g t g g c c c a t a t g t c g t a a g g g c t t g g c t g c a c c g g a a t c a g c a c g a a g t c g g c t a g c c g c c a c c g g g t a t a c a g c a t t c c c g a a c c g a c g t g g c c t t a g t c g t g c t t c a g c c g a

11,460 95 90 85 80 AKIASVALDAAQPAGDIVVF pVS1 StaA AKIASVALDAAQPAGDIVVF g c c t t g a t c g c g g a c a c a g c c a a g t c c g c c g c c t g g g g c g c t c c g t c g a t c a c t a c g a a g c g g a a c t a g c g c c t g t g t c g g t t c a g g c g g c g g a c c c c g c g a g g c a g c t a g t g a t g c t t c

11,520 75 70 65 60 DRRGIAKVDRDITPRDIGVV pVS1 StaA DRRGIAKVDRDITPRDIGVV t c g c g c c g g c c g a t g g c c t t c a c g t c g c g g t c a a t c g t c g g g c g g t c g a t g c c g a c a a c g a g c g c g g c c g g c t a c c g g a a g t g c a g c g c c a g t t a g c a g c c c g c c a g c t a c g g c t g t t g c

11,580 55 50 45 40 TLPQDERVAAWDRASGQPDS pVS1 StaA TLPQDERVAAWDRASGQPDS g t t a g c g g t t g a t c t t c c c g c a c g g c c g c c c a a t c g c g g g c a c t g c c c t g g g g a t c g g a a c a a t c g c c a a c t a g a a g g g c g t g c c g g c g g g t t a g c g c c c g t g a c g g g a c c c c t a g c c t t

11,640 35 30 25 20 DVLLVDAGALQLARALHTAI pVS1 StaA DVLLVDAGALQLARALHTAI t c g a c t a a c a g a a c a t c g g c c c c g g c g a g t t g c a g g g c g c g g g c t a g a t g g g t t g c g a t g a g c t g a t t g t c t t g t a g c c g g g g c c g c t c a a c g t c c c g c g c c c g a t c t a c c c a a c g c t a c

11,700 15 10 5 1 TTKGSGGKQNLVAIVKM pVS1 StaA TTKGSGGKQNLVAIVKM g t c g t c t t g c c t g a c c c g c c t t t c t g g t t a a g t a c a g c g a t a a c c t t c a t g c g t t c c c c t c a g c a g a a c g g a c t g g g c g g a a a g a c c a a t t c a t g t c g c t a t t g g a a g t a c g c a a g g g g a

11,760 t g c g t a t t t g t t t a t t t a c t c a t c g c a t c a t a t a c g c a g c g a c c g c a t g a c g c a a g c t g t a c g c a t a a a c a a a t a a a t g a g t a g c g t a g t a t a t g c g t c g c t g g c g t a c t g c g t t c g a c a

11,820 t t t a c t c a a a t a c a c a t c a c c t t t t t a g a c g g c g g c g c t c g g t t t c t t c a g c g g c c a a g c a a a t g a g t t t a t g t g t a g t g g a a a a a t c t g c c g c c g c g a g c c a a a g a a g t c g c c g g t t c g

11,880 t g g c c g g c c a g g c c g c c a g c t t g g c a t c a g a c a a a c c g g c c a g g a t t t c a t g c a g c c g c a a c c g g c c g g t c c g g c g g t c g a a c c g t a g t c t g t t t g g c c g g t c c t a a a g t a c g t c g g c g t

11,940 c g g t t g a g a c g t g c g c g g g c g g c t c g a a c a c g t a c c c g g c c g c g a t c a t c t c c g c c t c g a g c c a a c t c t g c a c g c g c c c g c c g a g c t t g t g c a t g g g c c g g c g c t a g t a g a g g c g g a g c t

12,000 *AQE t c t c t t c g g t a a t g a a a a a c g g t t c g t c c t g g c c g t c c t g g t g c g g t t t c a t g c t t g t t c a g a g a a g c c a t t a c t t t t t g c c a a g c a g g a c c g g c a g g a c c a c g c c a a a g t a c g a a c a a g

12,060 EQRENEAAALADAETLADER c t c t t g g c g t t c a t t c t c g g c g g c c g c c a g g g c g t c g g c c t c g g t c a a t g c g t c c t c a c g g a g a a c c g c a a g t a a g a g c c g c c g g c g g t c c c g c a g c c g g a g c c a g t t a c g c a g g a g t g c

12,120 FAGRRRAETPTVEESRELAR g a a g g c a c c g c g c c g c c t g g c c t c g g t g g g c g t c a c t t c c t c g c t g c g c t c a a g t g c g c g c t t c c g t g g c g c g g c g g a c c g g a g c c a c c c g c a g t g a a g g a g c g a c g c g a g t t c a c g c g c

12,180 YLTSRHVGLLAAAEKVTRGE g t a c a g g g t c g a g c g a t g c a c g c c a a g c a g t g c a g c c g c c t c t t t c a c g g t g c g g c c t t c c a t g t c c c a g c t c g c t a c g t g c g g t t c g t c a c g t c g g c g g a g a a a g t g c c a c g c c g g a a g

12,240 QDILERAHAIQAPTLTPRPG c t g g t c g a t c a g c t c g c g g g c g t g c g c g a t c t g t g c c g g g g t g a g g g t a g g g c g g g g g c c g a c c a g c t a g t c g a g c g c c c g c a c g c g c t a g a c a c g g c c c c a c t c c c a t c c c g c c c c c g g

12,300 FKVGRAKAAERGSRTRDIIL a a a c t t c a c g c c t c g g g c c t t g g c g g c c t c g c g c c c g c t c c g g g t g c g g t c g a t g a t t a g t t t g a a g t g c g g a g c c c g g a a c c g c c g g a g c g c g g g c g a g g c c c a c g c c a g c t a c t a a t c

12,360 SREFEAIGAFVTLVMRGAPT g g a a c g c t c g a a c t c g g c a a t g c c g g c g a a c a c g g t c a a c a c c a t g c g g c c g g c c g g c g t c c t t g c g a g c t t g a g c c g t t a c g g c c g c t t g t g c c a g t t g t g g t a c g c c g g c c g g c c g c a

12,420 TTDAWPEALSRLGAGAEQIR g g t g g t g t c g g c c c a c g g c t c t g c c a g g c t a c g c a g g c c c g c g c c g g c c t c c t g g a t g c g c c a c c a c a g c c g g g t g c c g a g a c g g t c c g a t g c g t c c g g g c g c g g c c g g a g g a c c t a c g c

12,480 EAIDLLDRTSRALRDLRTVT c t c g g c a a t g t c c a g t a g g t c g c g g g t g c t g c g g g c c a g g c g g t c t a g c c t g g t c a c t g t g a g c c g t t a c a g g t c a t c c a g c g c c c a c g a c g c c c g g t c c g c c a g a t c g g a c c a g t g a c a

12,540 VVDGPRLHDLMRALEPRDRR c a c a a c g t c g c c a g g g c g t a g g t g g t c a a g c a t c c t g g c c a g c t c c g g g c g g t c g c g c c t g t g t t g c a g c g g t c c c g c a t c c a c c a g t t c g t a g g a c c g g t c g a g g c c c g c c a g c g c g g a

12,600 TGTIKESFLKTCGAAHLEAR g g t g c c g g t g a t c t t c t c g g a a a a c a g c t t g g t g c a g c c g g c c g c g t g c a g t t c g g c c c g c c a c g g c c a c t a g a a g a g c c t t t t g t c g a a c c a c g t c g g c c g g c g c a c g t c a a g c c g g g c

12,660 QNTLDQDDTSVRAYGLLGAA t t g g t t g g t c a a g t c c t g g t c g t c g g t g c t g a c g c g g g c a t a g c c c a g c a g g c c a g c g g c a a c c a a c c a g t t c a g g a c c a g c a g c c a c g a c t g c g c c c g t a t c g g g t c g t c c g g t c g c c g

12,720 ASKNM g g c g c t c t t g t t c a t g g c g t a a t g t c t c c g g t t c t a g t c g c a a g t a t t c t a c t t t a t g c g c c g c g a g a a c a a g t a c c g c a t t a c a g a g g c c a a g a t c a g c g t t c a t a a g a t g a a a t a c g c

12,780 a c t a a a a c a c g c g a c a a g a a a a c g c c a g g a a a a g g g c a g g g c g g c a g c c t g t c g c g t a a c t g a t t t t g t g c g c t g t t c t t t t g c g g t c c t t t t c c c g t c c c g c c g t c g g a c a g c g c a t t g

12,840 t t a g g a c t t g t g c g a c a t g t c g t t t t c a g a a g a c g g c t g c a c t g a a c g t c a g a a g c c g a c a a t c c t g a a c a c g c t g t a c a g c a a a a g t c t t c t g c c g a c g t g a c t t g c a g t c t t c g g c t g

12,900 t g c a c t a t a g c a g c g g a g g g g t t g g a t c a a a g t a c t t t g a t c c c g a g g g g a a c c c t g t g g a c g t g a t a t c g t c g c c t c c c c a a c c t a g t t t c a t g a a a c t a g g g c t c c c c t t g g g a c a c c

3ʹ 5ʹ 12,937 t t g g c a t g c a c a t a c a a a t g g a c g a a c g g a t a a a c c t a a c c g t a c g t g t a t g t t t a c c t g c t t g c c t a t t t g g a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.