Addgene pT040 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pT040


Map View

Bpu10I (5035) BsmBI - Esp3I (4927) BsmI (4667) NcoI (4510) PpuMI (4458) XcmI (4433) BsgI (4251) NdeI (4218) BlpI (4076) BglII (3836) BsrGI (3764) BtgZI (3763) PflFI - Tth111I (3208) PluTI (2958) SfoI (2956) NarI (2955) KasI (2954) M13/pUC Forward (2808 .. 2830) M13 Forward (2799 .. 2816) M13 fwd T7 (2773 .. 2792) T7 promoter SacI (2764) Eco53kI (2762) SacII (2755) BclI * (2478) AmpR promoter XmnI (319) Amp-R (350 .. 369) BsrFI (834) AhdI (919) pBR322ori-F (1652 .. 1671) DrdI (1705) L4440 (1905 .. 1922) CAP binding site lac promoter M13/pUC Reverse (2118 .. 2140) lac operator M13 Reverse (2137 .. 2153) M13 rev T3 promoter Acc65I (2204) KpnI (2208) pBluescriptKS (2224 .. 2240) SalI (2229) KS primer EcoRI (2256) AvaI - BsoBI - PaeR7I - PspXI - XhoI (2274) BamHI (2286) SpeI (2292) SK primer pBluescriptSK (2287 .. 2303) EagI (2305) SUP4 terminator SwaI (2463) BsaBI * (2477) pT040 5186 bp

Sequence View

5ʹ 3ʹ 60 AmpR promoter c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g

120 AmpR promoter a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t

180 1 5 10 15 signal sequence MSIQHFRVALIPFFAA AmpR promoter AmpR MSIQHFRVALIPFFAA a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a

240 20 signal sequence FCLPVFA 25 30 35 HPETLVKVKDAED AmpR FCLPVFAHPETLVKVKDAED t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g

300 40 45 50 55 QLGARVGYIELDLNSGKILE AmpR QLGARVGYIELDLNSGKILE a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t

XmnI 360 60 65 70 75 SFRPEERFPMMSTFKVLLCG AmpR C G A T A C A C C G C Amp-R SFRPEERFPMMSTFKVLLCG g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c

420 80 85 90 95 AVLSRIDAGQEQLGRRIHYS AmpR G C C A T A A T A Amp-R AVLSRIDAGQEQLGRRIHYS c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g

480 100 105 110 115 QNDLVEYSPVTEKHLTDGMT AmpR QNDLVEYSPVTEKHLTDGMT a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c

540 120 125 130 135 VRELCSAAITMSDNTAANLL AmpR VRELCSAAITMSDNTAANLL t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g

600 140 145 150 155 LTTIGGPKELTAFLHNMGDH AmpR LTTIGGPKELTAFLHNMGDH t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c

660 160 165 170 175 VTRLDRWEPELNEAIPNDER AmpR VTRLDRWEPELNEAIPNDER t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c

720 180 185 190 195 DTTMPVAMATTLRKLLTGEL AmpR DTTMPVAMATTLRKLLTGEL a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g

780 200 205 210 215 LTLASRQQLIDWMEADKVAG AmpR LTLASRQQLIDWMEADKVAG t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g

BsrFI 840 220 225 230 235 PLLRSALPAGWFIADKSGAG AmpR PLLRSALPAGWFIADKSGAG c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c

900 240 245 250 255 ERGSRGIIAALGPDGKPSRI AmpR ERGSRGIIAALGPDGKPSRI a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c

AhdI 960 260 265 270 275 VVIYTTGSQATMDERNRQIA AmpR VVIYTTGSQATMDERNRQIA t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c

1020 280 285 EIGASLIKHW* AmpR EIGASLIKHW* a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g

1080 t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c

1140 a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c

1200 ori t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g

1260 ori a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g

1320 ori t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a

1380 ori a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g

1440 ori t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a

1500 ori c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g

1560 ori a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c

1620 ori a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c

1680 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c

DrdI 1740 ori t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t

1800 ori g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a

1860 t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a

1920 A G C G A G T C A G T G A G C G L4440 t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c

1980 A G L4440 a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a

2040 CAP binding site a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t

2100 -35 CAP binding site lac promoter a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t

2160 -10 lac promoter lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a

Acc65IKpnI 2220 T3 promoter a c g c c a a g c t c g a a a t t a a c c c t c a c t a a a g g g a a c a a a a g c t g g t a c c g g g c c c c c c c t t g c g g t t c g a g c t t t a a t t g g g a g t g a t t t c c c t t g t t t t c g a c c a t g g c c c g g g g g g g a

PaeR7IBsoBIPspXIXhoIAvaIEcoRISalI 2280 KS primer T C G A G G T C G A C G G T A T C pBluescriptKS c g a t c g a g g t c g a c g g t a t c g a t a a g c t t g a t a t c g a a t t c c t g c a g c c c c c g c t c g a g c g c t a g c t c c a g c t g c c a t a g c t a t t c g a a c t a t a g c t t a a g g a c g t c g g g g g c g a g c t c g

EagISpeIBamHI 2340 SK primer C T A G G T G A T C A A G A T C T pBluescriptSK g g g g g g g a t c c a c t a g t t c t a g a g c g g c c g t g g t a t c g t t t a g a t t g g c a a t t a c a g t g t c c c c c c c t a g g t g a t c a a g a t c t c g c c g g c a c c a t a g c a a a t c t a a c c g t t a a t g t c a c a

2400 SUP4 terminator c t t a g c t c a c a t g c t t a t a a c t a a t t a c a t g a c t c g a a g a c a t a a a a a a c a a a a a a a g c a g a a t c g a g t g t a c g a a t a t t g a t t a a t g t a c t g a g c t t c t g t a t t t t t t g t t t t t t t c g t

2460 c c a c c g a c t c g g t g c c a c t t t t t c a a g t t g a t a a c g g a c t a g c c t t a t t t t a a c t t g c t a g g t g g c t g a g c c a c g g t g a a a a a g t t c a a c t a t t g c c t g a t c g g a a t a a a a t t g a a c g a t

BsaBI*BclI*SwaI 2520 SNR52 promoter t t t a a a t g c a t c g a t t c t g a t c a t t t a t c t t t c a c t g c g g a g a a g t t t c g a a c g c c g a a a a a a t t t a c g t a g c t a a g a c t a g t a a a t a g a a a g t g a c g c c t c t t c a a a g c t t g c g g c t t t

2580 SNR52 promoter c a t g c g c a c c a a c t t t c a c t t c t a c a g c g t t t g a c c a a a a t c t t t t g a a c a g a a c a t t g t g t a c g c g t g g t t g a a a g t g a a g a t g t c g c a a a c t g g t t t t a g a a a a c t t g t c t t g t a a c a

2640 SNR52 promoter a g g g t g t g a a a a a a t g c g c a c c t t t a c c g c t a g c c c a a g a g g g c a c t a c a a a a t c t a g a g t c c c a c a c t t t t t t a c g c g t g g a a a t g g c g a t c g g g t t c t c c c g t g a t g t t t t a g a t c t c

2700 SNR52 promoter t t g t a c t t c a a a c g t a c a t g t a a t c a c c t t g t a t a t a c t c g a a a g a a a a c a t c a a g t t t c a a c a t g a a g t t t g c a t g t a c a t t a g t g g a a c a t a t a t g a g c t t t c t t t t g t a g t t c a a a g

SacII 2760 SNR52 promoter t g t a t a a a t a t g a g t g a a a g c a t a a t c a t a c a t t a t c t t t t c a a a g a t t g t c c g c g g t g g a c a t a t t t a t a c t c a c t t t c g t a t t a g t a t g t a a t a g a a a a g t t t c t a a c a g g c g c c a c c

Eco53kISacI 2820 T7 promoter M13 fwd G G G A T A T C A C T C A G C A T A A T T7 T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A M13/pUC Forward a g c t c c a a t t c g c c c t a t a g t g a g t c g t a t t a c a a t t c a c t g g c c g t c g t t t t a c a a c g t t c g a g g t t a a g c g g g a t a t c a c t c a g c a t a a t g t t a a g t g a c c g g c a g c a a a a t g t t g c a

2880 G C A C T G A C C C M13/pUC Forward c g t g a c t g g g a a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g

2940 g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c g g t c g a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g

KasINarISfoIPluTI 3000 c t g a a t g g c g a a t g g c g c c t g a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a g a c t t a c c g c t t a c c g c g g a c t a c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t

3060 c a c c g c a t a a c t a g c t t t t a a c t t g t a t c c t a g g t t a t c t a t g c t g t c t c a c c a t a g a g a g t g g c g t a t t g a t c g a a a a t t g a a c a t a g g a t c c a a t a g a t a c g a c a g a g t g g t a t c t c t

3120 a t a t t a c c t a t t t c a g a a t g t a t g t c c a t g a t t c g c c g g g t a a a t a c a t a t a a t a c a c a a t a t a a t g g a t a a a g t c t t a c a t a c a g g t a c t a a g c g g c c c a t t t a t g t a t a t t a t g t g t t

3180 a t c t g g c t t a a t a a a g t c t a t a a t a t a t c t c a t a a a g a a g t g c t a a a t t g g c t a g t g c t a t a g a c c g a a t t a t t t c a g a t a t t a t a t a g a g t a t t t c t t c a c g a t t t a a c c g a t c a c g a t

Tth111IPflFI 3240 t a t a t t t t t a a g a a a a t t t c t t t t g a c t a a g t c c a t a t c g a c t t t g t a a a a g t t c a c t t t a t a t a a a a a t t c t t t t a a a g a a a a c t g a t t c a g g t a t a g c t g a a a c a t t t t c a a g t g a a a

3300 a g c a t a c a t a t a t t a c a c g a g c c a g a a a t t g t a a c t t t t g c c t a a a a t c a c a a a t t g c a a t c g t a t g t a t a t a a t g t g c t c g g t c t t t a a c a t t g a a a a c g g a t t t t a g t g t t t a a c g t t

3360 CEN4 a a t t t a a t t g c t t g c a a a a g g t c a c a t g c t t a t a a t c a a c t t t t t t a a a a a t t t a a a a t a t t a a a t t a a c g a a c g t t t t c c a g t g t a c g a a t a t t a g t t g a a a a a a t t t t t a a a t t t t a t

3420 CEN4 c t t t t t t a t t t t t t a t t t t t a a a c a t a a a t g a a a t a a t t t a t t t a t t g t t t a t g a t t a c c g a a a a a a t a a a a a a t a a a a a t t t g t a t t t a c t t t a t t a a a t a a a t a a c a a a t a c t a a t g g

3480 CEN4 g a a a c a t a a a a c c t g c t c a a g a a a a a g a a a c t g t t t t g t c c t t g g a a a a a a a g c a c t a c c c t t t g t a t t t t g g a c g a g t t c t t t t t c t t t g a c a a a a c a g g a a c c t t t t t t t c g t g a t g g

3540 CEN4 t a g g a g c g g c c a a a a t g c c g a g g c t t t c a t a g c t t a a a c t c t t t a c a g a a a a t a g g c a t t a t c c t c g c c g g t t t t a c g g c t c c g a a a g t a t c g a a t t t g a g a a a t g t c t t t t a t c c g t a a

3600 CEN4 a t a g a t c a g t t c g a g t t t t c t t a t t c t t c c t t c c g g t t t t a t c g t c a c a g t t t t a c a g t a t a t c t a g t c a a g c t c a a a a g a a t a a g a a g g a a g g c c a a a a t a g c a g t g t c a a a a t g t c a t

3660 CEN4 ARS1 a a t a a g t a t c a c c t c t t a g a g t t g g c g g t t g t t t g c a a g a c c g a g a a a a g g c t a g c a a g a t t a t t c a t a g t g g a g a a t c t c a a c c g c c a a c a a a c g t t c t g g c t c t t t t c c g a t c g t t c t

3720 ARS1 a t c g g g t c a t t g t a g c g t a t g c g c c t g t g a a c a t t c t c t t c a a c a a g t t t g a t t c c a t t g t a g c c c a g t a a c a t c g c a t a c g c g g a c a c t t g t a a g a g a a g t t g t t c a a a c t a a g g t a a c

BtgZIBsrGI 3780 ARS1 c g g t g a a a t g g t a a a a g t c a a c c c c c t g c g a t g t a t a t t t t c c t g t a c a a t c a a t c a a a a g c c a c t t t a c c a t t t t c a g t t g g g g g a c g c t a c a t a t a a a a g g a c a t g t t a g t t a g t t t t

BglII 3840 ARS1 a g c c a a a t g a t t t a g c a t t a t c t t t a c a t c t t g t t a t t t t a c a g a t t t t a t g t t t a g a t c t c g g t t t a c t a a a t c g t a a t a g a a a t g t a g a a c a a t a a a a t g t c t a a a a t a c a a a t c t a g

3900 ARS1 t t t t a t g c t t g c t t t t c a a a a g g c c t g c a g g c a a g t g c a c a a a c a a t a c t t a a a t a a a t a a a a a t a c g a a c g a a a a g t t t t c c g g a c g t c c g t t c a c g t g t t t g t t a t g a a t t t a t t t a t

3960 ARS1 c t a c t c a g t a a t a a c c t a t t t c t t a g c a t t t t t g a c g a a a t t t g c t a t t t t g t t a g a g t c g a t g a g t c a t t a t t g g a t a a a g a a t c g t a a a a a c t g c t t t a a a c g a t a a a a c a a t c t c a g

4020 ARS1 t t t t a c a c c a t t t g t c t c c a c a c c t c c g c t t a c a t c a a c a c c a a t a a c g c c a t t t a a t c t a a a a t g t g g t a a a c a g a g g t g t g g a g g c g a a t g t a g t t g t g g t t a t t g c g g t a a a t t a g a

BlpI 4080 ARS1 a a g c g c a t c a c c a a c a t t t t c t g g c g t c a g t c c a c c a g c t a a c a t a a a a t g t a a g c t a a g t t c g c g t a g t g g t t g t a a a a g a c c g c a g t c a g g t g g t c g a t t g t a t t t t a c a t t c g a t t c

4140 URA3 promoter c t t t t c a a t t c a a t t c a t c a t t t t t t t t t t a t t c t t t t t t t t g a t t t c g g t t t c t t t g a a g a a a a g t t a a g t t a a g t a g t a a a a a a a a a a t a a g a a a a a a a a c t a a a g c c a a a g a a a c t t

4200 URA3 promoter a t t t t t t t g a t t c g g t a a t c t c c g a a c a g a a g g a a g a a c g a a g g a a g g a g c a c a g a c t t a t a a a a a a a c t a a g c c a t t a g a g g c t t g t c t t c c t t c t t g c t t c c t t c c t c g t g t c t g a a t

BsgINdeI 4260 URA3 promoter g a t t g g t a t a t a t a c g c a t a t g t a g t g t t g a a g a a a c a t g a a a t t g c c c a g t a t t c t t a a c t a a c c a t a t a t a t g c g t a t a c a t c a c a a c t t c t t t g t a c t t t a a c g g g t c a t a a g a a t t

4320 1 5 MSKAT URA3 promoter URA3 MSKAT c c c a a c t g c a c a g a a c a a a a a c c t g c a g g a a a c g a a g a t a a a t c a t g t c g a a a g c t a c a t g g g t t g a c g t g t c t t g t t t t t g g a c g t c c t t t g c t t c t a t t t a g t a c a g c t t t c g a t g t a

4380 10 15 20 25 YKERAATHPSPVAAKLFNIM URA3 YKERAATHPSPVAAKLFNIM *GLGTAALSNLIMC a t a a g g a a c g t g c t g c t a c t c a t c c t a g t c c t g t t g c t g c c a a g c t a t t t a a t a t c a t g c t a t t c c t t g c a c g a c g a t g a g t a g g a t c a g g a c a a c g a c g g t t c g a t a a a t t a t a g t a c g

XcmI 4440 30 35 40 45 HEKQTNLCASLDVRTTKELL URA3 HEKQTNLCASLDVRTTKELL SFCVFKHAENSTRVVLSNSS a c g a a a a g c a a a c a a a c t t g t g t g c t t c a t t g g a t g t t c g t a c c a c c a a g g a a t t a c t g g t g c t t t t c g t t t g t t t g a a c a c a c g a a g t a a c c t a c a a g c a t g g t g g t t c c t t a a t g a c c

PpuMI 4500 50 55 60 65 ELVEALGPKICLLKTHVDIL URA3 ELVEALGPKICLLKTHVDIL NTSANPGLIQKSFVCTSIKV a g t t a g t t g a a g c a t t a g g t c c c a a a a t t t g t t t a c t a a a a a c a c a t g t g g a t a t c t t g a t c a a t c a a c t t c g t a a t c c a g g g t t t t a a a c a a a t g a t t t t t g t g t a c a c c t a t a g a a c t

NcoI 4560 70 75 80 85 TDFSMEGTVKPLKALSAKYN URA3 TDFSMEGTVKPLKALSAKYN SKEMSPVTLGSFANDALYLK c t g a t t t t t c c a t g g a g g g c a c a g t t a a g c c g c t a a a g g c a t t a t c c g c c a a g t a c a a t t g a c t a a a a a g g t a c c t c c c g t g t c a a t t c g g c g a t t t c c g t a a t a g g c g g t t c a t g t t a a

4620 90 95 100 105 FLLFEDRKFADIGNTVKLQY URA3 FLLFEDRKFADIGNTVKLQY KSKSSLFNASMPLVTLNCYE t t t t a c t c t t c g a a g a c a g a a a a t t t g c t g a c a t t g g t a a t a c a g t c a a a t t g c a g t a c t a a a a t g a g a a g c t t c t g t c t t t t a a a c g a c t g t a a c c a t t a t g t c a g t t t a a c g t c a t g a

BsmI 4680 110 115 120 125 SAGVYRIAEWADITNAHGVV URA3 SAGVYRIAEWADITNAHGVV APTYLIASHASMVFACPTTP c t g c g g g t g t a t a c a g a a t a g c a g a a t g g g c a g a c a t t a c g a a t g c a c a c g g t g t g g t g g g a c g c c c a c a t a t g t c t t a t c g t c t t a c c c g t c t g t a a t g c t t a c g t g t g c c a c a c c a c c

4740 130 135 140 145 GPGIVSGLKQAAEEVTKEPR URA3 GPGIVSGLKQAAEEVTKEPR GPITLPKFCAASSTVFSGLP g c c c a g g t a t t g t t a g c g g t t t g a a g c a g g c g g c a g a a g a a g t a a c a a a g g a a c c t a g a g c g g g t c c a t a a c a a t c g c c a a a c t t c g t c c g c c g t c t t c t t c a t t g t t t c c t t g g a t c t c

4800 150 155 160 165 GLLMLAELSCKGSLSTGEYT URA3 GLLMLAELSCKGSLSTGEYT RKINASNDHLPERDVPSYVL g c c t t t t g a t g t t a g c a g a a t t g t c a t g c a a g g g c t c c c t a t c t a c t g g a g a a t a t a c t a c g g a a a a c t a c a a t c g t c t t a a c a g t a c g t t c c c g a g g g a t a g a t g a c c t c t t a t a t g a t

4860 170 175 180 185 KGTVDIAKSDKDFVIGFIAQ URA3 KGTVDIAKSDKDFVIGFIAQ PVTSM a g g g t a c t g t t g a c a t t g c g a a g a g c g a c a a a g a t t t t g t t a t c g g c t t t a t t g c t c a a a t c c c a t g a c a a c t g t a a c g c t t c t c g c t g t t t c t a a a a c a a t a g c c g a a a t a a c g a g t t t

4920 190 195 200 205 RDMGGRDEGYDWLIMTPGVG URA3 RDMGGRDEGYDWLIMTPGVG g a g a c a t g g g t g g a a g a g a t g a a g g t t a c g a t t g g t t g a t t a t g a c a c c c g g t g t g g g t t c t c t g t a c c c a c c t t c t c t a c t t c c a a t g c t a a c c a a c t a a t a c t g t g g g c c a c a c c c a a

BsmBIEsp3I 4980 210 215 220 225 LDDKGDALGQQYRTVDDVVS URA3 LDDKGDALGQQYRTVDDVVS t a g a t g a c a a g g g a g a c g c a t t g g g t c a a c a g t a t a g a a c c g t g g a t g a t g t g g t c t c t a a t c t a c t g t t c c c t c t g c g t a a c c c a g t t g t c a t a t c t t g g c a c c t a c t a c a c c a g a g a t

Bpu10I 5040 230 235 240 245 TGSDIIIVGRGLFAKGRDAK URA3 TGSDIIIVGRGLFAKGRDAK c a g g a t c t g a c a t t a t t a t t g t t g g a a g a g g a c t a t t t g c a a a g g g a a g g g a t g c t a a g g g t c c t a g a c t g t a a t a a t a a c a a c c t t c t c c t g a t a a a c g t t t c c c t t c c c t a c g a t t c c

5100 250 255 260 265 VEGERYRKAGWEAYLRRCGQ URA3 VEGERYRKAGWEAYLRRCGQ t a g a g g g t g a a c g t t a c a g a a a a g c a g g c t g g g a a g c a t a t t t g a g a a g a t g c g g c c a g c a t c t c c c a c t t g c a a t g t c t t t t c g t c c g a c c c t t c g t a t a a a c t c t t c t a c g c c g g t c g

5160 QN* URA3 QN* a a a a c t a a a a a a c t g t a t t a t a a g t a a a t g c a t g t a t a c t a a a c t c a c a a a t t a g a g c t t t t t t g a t t t t t t g a c a t a a t a t t c a t t t a c g t a c a t a t g a t t t g a g t g t t t a a t c t c g a a

3ʹ 5ʹ 5186 c a a t t t a a t t a t a t c a g t t a t t a c c c g t t a a a t t a a t a t a g t c a a t a a t g g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.