Addgene BASIC_13_ATG-GFP-nostop - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: BASIC_13_ATG-GFP-nostop


Map View

EagI - NotI (3689) T7 (3665 .. 3684) BfuAI - BspMI (3570) DraIII (3485) BbsI (3411) PstI (3369) pBRforEco (3341 .. 3359) XmnI (2980) Amp-R (2931 .. 2950) ScaI (2861) TatI (2859) PvuI (2751) FspI (2603) NmeAIII (2529) BglI (2501) BmrI (2421) AhdI (2381) AlwNI (1904) PaeR7I - PspXI - XhoI (3712) AgeI (166) HincII (209) PfoI * (296) RsrII (589) BlpI (637) BclI * (663) BanII (742) XbaI (763) NcoI (794) BspDI - ClaI (804) HindIII (1010) BbvCI - Bpu10I (1104) BsmI (1108) BsmBI - Esp3I (1131) PmeI (1150) BspQI - SapI (1372) L4440 (1378 .. 1395) pBR322ori-F (1629 .. 1648) BseYI (1792) PspFI (1796) BASIC_13_ATG-GFP-nostop 3730 bp

Sequence View

60 1 5 10 MRKGEELFTGVVPI superfolder GFP MRKGEELFTGVVPI RTPRQGHTFAFFQERAYNRN t c t g g t g g g t c t c t g t c c a t g c g t a a a g g c g a a g a a c t g t t c a c g g g c g t a g t t c c g a t t a g a c c a c c c a g a g a c a g g t a c g c a t t t c c g c t t c t t g a c a a g t g c c c g c a t c a a g g c t a a

120 15 20 25 30 LVELDGDVNGHKFSVRGEGE superfolder GFP LVELDGDVNGHKFSVRGEGE QDLQVAIHVTMLKANATFTL c t g g t c g a g c t g g a c g g c g a t g t g a a c g g t c a t a a g t t t a g c g t t c g c g g t g a a g g t g a g g a c c a g c t c g a c c t g c c g c t a c a c t t g c c a g t a t t c a a a t c g c a a g c g c c a c t t c c a c t c

AgeI 180 35 40 45 50 GDATNGKLTLKFICTTGKLP superfolder GFP GDATNGKLTLKFICTTGKLP AVRGVAFQGQLEDAGGTFQR g g c g a c g c g a c c a a c g g c a a a c t g a c c c t g a a g t t c a t c t g c a c c a c c g g t a a a c t g c c g c c g c t g c g c t g g t t g c c g t t t g a c t g g g a c t t c a a g t a g a c g t g g t g g c c a t t t g a c g g c

HincII 240 55 60 65 70 VPWPTLVTTLTYGVQCFARY superfolder GFP VPWPTLVTTLTYGVQCFARY HRPRGQHRRQRIAHLTKRTI g t g c c t t g g c c g a c c t t g g t g a c g a c g t t g a c g t a t g g c g t g c a g t g t t t t g c g c g t t a t c a c g g a a c c g g c t g g a a c c a c t g c t g c a a c t g c a t a c c g c a c g t c a c a a a a c g c g c a a t a

PfoI* 300 75 80 85 90 PDHMKQHDFFKSAMPEGYVQ superfolder GFP PDHMKQHDFFKSAMPEGYVQ RVVHFLVIEEFRRHRLTVDL c c g g a c c a c a t g a a a c a a c a c g a t t t c t t c a a a t c t g c g a t g c c g g a g g g t t a c g t c c a g g g c c t g g t g t a c t t t g t t g t g c t a a a g a a g t t t a g a c g c t a c g g c c t c c c a a t g c a g g t c

360 95 100 105 110 ERTISFKDDGTYKTRAEVKF superfolder GFP ERTISFKDDGTYKTRAEVKF LTGNGELIIASVFSACLNLK g a g c g t a c c a t t t c c t t c a a g g a t g a t g g c a c t t a c a a a a c t c g c g c a g a g g t t a a g t t t c t c g c a t g g t a a a g g a a g t t c c t a c t a c c g t g a a t g t t t t g a g c g c g t c t c c a a t t c a a a

420 115 120 125 130 EGDTLVNRIELKGIDFKEDG superfolder GFP EGDTLVNRIELKGIDFKEDG FTVRQDITDFQLTDVKFLIT g a a g g t g a c a c g c t g g t c a a t c g t a t c g a a t t g a a g g g t a t c g a c t t t a a a g a g g a t g g t c t t c c a c t g t g c g a c c a g t t a g c a t a g c t t a a c t t c c c a t a g c t g a a a t t t c t c c t a c c a

480 135 140 145 150 NILGHKLEYNFNSHNVYITA superfolder GFP NILGHKLEYNFNSHNVYITA VNQAMFQLIVEVAMINVNRC a a c a t t c t g g g c c a t a a a c t g g a g t a t a a c t t c a a c a g c c a t a a t g t t t a c a t t a c g g c a t t g t a a g a c c c g g t a t t t g a c c t c a t a t t g a a g t t g t c g g t a t t a c a a a t g t a a t g c c g t

540 155 160 165 170 DKQKNGIKANFKIRHNVEDG superfolder GFP DKQKNGIKANFKIRHNVEDG VLLLVADLGIELNAVINLVT g a c a a g c a a a a g a a c g g c a t c a a g g c c a a t t t c a a g a t t c g c c a c a a t g t t g a g g a c g g t c t g t t c g t t t t c t t g c c g t a g t t c c g g t t a a a g t t c t a a g c g g t g t t a c a a c t c c t g c c a

RsrII 600 175 180 185 190 SVQLADHYQQNTPIGDGPVL superfolder GFP SVQLADHYQQNTPIGDGPVL ADLQGVM a g c g t c c a a c t g g c c g a c c a t t a c c a g c a g a a c a c c c c a a t t g g t g a c g g t c c g g t t t t g t c g c a g g t t g a c c g g c t g g t a a t g g t c g t c t t g t g g g g t t a a c c a c t g c c a g g c c a a a a c

BlpI 660 195 200 205 210 LPDNHYLSTQSVLSKDPNEK superfolder GFP LPDNHYLSTQSVLSKDPNEK c t g c c g g a t a a t c a c t a t c t g a g c a c c c a a a g c g t g c t g a g c a a a g a t c c g a a c g a a a a a g a c g g c c t a t t a g t g a t a g a c t c g t g g g t t t c g c a c g a c t c g t t t c t a g g c t t g c t t t t t

BclI* 720 215 220 225 230 RDHMVLLEFVTAAGITHGMD superfolder GFP RDHMVLLEFVTAAGITHGMD *MTRSSNTVAAPMVWPIS c g t g a t c a c a t g g t c c t g c t g g a a t t t g t g a c c g c t g c g g g c a t c a c c c a c g g t a t g g a c g c a c t a g t g t a c c a g g a c g a c c t t a a a c a c t g g c g a c g c c c g t a g t g g g t g c c a t a c c t g

XbaIBanII 780 235 ELYK (in frame with superfolder GFP) RPGSGDLSYLSRRSPT superfolder GFP ELYKRPGSGDLSYLSRRSPT SSYLRGPEPSRDYRELLDGV g a g c t g t a t a a g c g t c c g g g c t c g g g a g a c c t a t c g t a t c t t t c t a g a a g a t c t c c t a c a c t c g a c a t a t t c g c a g g c c c g a g c c c t c t g g a t a g c a t a g a a a g a t c t t c t a g a g g a t g t

BspDIClaINcoI 840 (in frame with superfolder GFP) IFSAAMENRCSSFILSRFSG IFSAAMENRCSSFILSRFSG INEAAMSFRHEEKIRELNEP a t a t t c t c a g c t g c c a t g g a a a a t c g a t g t t c t t c t t t t a t t c t c t c a a g a t t t t c a g g c t a t a a g a g t c g a c g g t a c c t t t t a g c t a c a a g a a g a a a a t a a g a g a g t t c t a a a a g t c c g

900 (in frame with superfolder GFP) CILKLILRTMLTTSSGTVVG CILKLILRTMLTTSSGTVVG QINFSINLVISVVEDPVTTP t g t a t a t t a a a a c t t a t a t t a a g a a c t a t g c t a a c c a c c t c a t c a g g a a c c g t t g t a g g t a c a t a t a a t t t t g a a t a t a a t t c t t g a t a c g a t t g g t g g a g t a g t c c t t g g c a a c a t c c a

960 (in frame with superfolder GFP) GVGFLGNRLS* GVGFLGNRLS* PTPKRPLRSEHFSRALYEIH g g c g t g g g t t t t c t t g g c a a t c g a c t c t c a t g a a a a c t a c g a g c t a a a t a t t c a a t a t g t c c g c a c c c a a a a g a a c c g t t a g c t g a g a g t a c t t t t g a t g c t c g a t t t a t a a g t t a t a c a

HindIII 1020 EEQGVKNQMKKSRPKSCAEP t c c t c t t g a c c a a c t t t a t t c t g c a t t t t t t t t g a a c g a g g t t t a g a g c a a g c t t c a g g a a g g a g a a c t g g t t g a a a t a a g a c g t a a a a a a a a c t t g c t c c a a a t c t c g t t c g a a g t c c t

1080 FQSLFKILFKFKSSLEPNIA a a c t g a g a c a g g a a t t t t a t t a a a a a t t t a a a t t t t g a a g a a a g t t c a g g g t t a a t a g c a t t g a c t c t g t c c t t a a a a t a a t t t t t a a a t t t a a a a c t t c t t t c a a g t c c c a a t t a t c g t

BsmBIEsp3IBpu10IBbvCIBsmI 1140 DMKQKALEEANKVFSTEKSM t c c a t t t t t t g c t t t g c a a g t t c c t c a g c a t t c t t a a c a a a a g a c g t c t c t t t t g a c a t g a g g t a a a a a a c g a a a c g t t c a a g g a g t c g t a a g a a t t g t t t t c t g c a g a g a a a a c t g t a c

PmeI 1200 -10 lac UV5 promoter t t t a a a g t t t a a a c c t c c t g t g t g a a a t t a t t a t c c g c t c a t a a t t c c a c a c a t t a t a c g a a a t t t c a a a t t t g g a g g a c a c a c t t t a a t a a t a g g c g a g t a t t a a g g t g t g t a a t a t g c

1260 -35 lac UV5 promoter CAP binding site a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a

1320 t g c g t t g c g c t c a c t g c c a a t t g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a c g c a a c g c g a g t g a c g g t t a a c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t

BspQISapI 1380 G A G L4440 a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g

1440 C G A G T G A C T G A G C G A L4440 g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t

1500 g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t

1560 ori a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a

1620 ori c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g

1680 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g

1740 ori g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g

BseYIPspFI 1800 ori t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c

1860 ori t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t

AlwNI 1920 ori g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c

1980 ori c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t

2040 ori c a c t a g a a g g a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g g t g a t c t t c c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c

2100 ori a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c

2160 ori c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g

2220 g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g

2280 a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c

2340 285 280 *WHKILSAGIE AmpR *WHKILSAGIE t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g

AhdI 2400 275 270 265 260 AIQRNREDMTAQSGTTYIVV AmpR AIQRNREDMTAQSGTTYIVV a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g

BmrI 2460 255 250 245 240 IRSPKGDPGLAAIIGRSGRE AmpR IRSPKGDPGLAAIIGRSGRE g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g

BglI 2520 235 230 225 220 GAGSKDAIFWGAPLASRLLP AmpR GAGSKDAIFWGAPLASRLLP a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c

NmeAIII 2580 215 210 205 200 GAVKDAEMWDILQQRSALTL AmpR GAVKDAEMWDILQQRSALTL t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c

FspI 2640 195 190 185 180 LEGTLLKRLTTAMAVPMTTD AmpR LEGTLLKRLTTAMAVPMTTD t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g

2700 175 170 165 160 REDNPIAENLEPEWRDLRTV AmpR REDNPIAENLEPEWRDLRTV a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g

PvuI 2760 155 150 145 140 HDGMNHLFATLEKPGGITTL AmpR HDGMNHLFATLEKPGGITTL a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c

2820 135 130 125 120 LLNAATNDSMTIAASCLERV AmpR LLNAATNDSMTIAASCLERV a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g

TatIScaI 2880 115 110 105 100 TMGDTLHKETVPSYEVLDNQ AmpR TMGDTLHKETVPSYEVLDNQ t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c

2940 95 90 85 80 SYHIRRGLQEQGADIRSLVA AmpR A T A A T A C C G C Amp-R SYHIRRGLQEQGADIRSLVA a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g

XmnI 3000 75 70 65 60 GCLLVKFTSMMPFREEPRFS AmpR G C C A C A T A G C Amp-R GCLLVKFTSMMPFREEPRFS g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a

3060 55 50 45 40 ELIKGSNLDLEIYGVRAGLQ AmpR ELIKGSNLDLEIYGVRAGLQ c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c

3120 35 30 25 DEADKVKVLTEPH 20 signal sequence AFVPLCF AmpR DEADKVKVLTEPHAFVPLCF a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t

3180 15 10 5 1 signal sequence AAFFPILAVRFHQISM AmpR AmpR promoter AAFFPILAVRFHQISM t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a

3240 AmpR promoter t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c

3300 AmpR promoter t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t

3360 A A T A G G C G T A T C A C G A G G C pBRforEco c g t c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c c g c a g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g g

BbsIPstI 3420 g c c c c t g c a g c c g a a t t a t a t t a t t t t t g c c a a a t a a t t t t t a a c a a a a g c t c t g a a g t c c g g g g a c g t c g g c t t a a t a t a a t a a a a a c g g t t t a t t a a a a a t t g t t t t c g a g a c t t c a g

3480 t t c t t c a t t t a a a t t c t t a g a t g a t a c t t c a t c t g g a a a a t t g t c c c a a t t a g t a g c a t c a a g a a g t a a a t t t a a g a a t c t a c t a t g a a g t a g a c c t t t t a a c a g g g t t a a t c a t c g t a g

DraIII 3540 a c g c t g t g a g t a a g t t c t a a a c c a t t t t t t t a t t g t t g t a t t a t c t c t a a t c t t a c t a c t t g c g a c a c t c a t t c a a g a t t t g g t a a a a a a a t a a c a a c a t a a t a g a g a t t a g a a t g a t g a

BspMIBfuAI 3600 c g a t g a g t t t t c g g t a t t a t c t c t a t t t t t a a c t t g g a g c a g g t t c c a t t c a t t g t t t t t g c t a c t c a a a a g c c a t a a t a g a g a t a a a a a t t g a a c c t c g t c c a a g g t a a g t a a c a a a a a

3660 t t c a t c a t a g t g a a t a a a a t c a a c t g c t t t a a c a c t t g t g c c t g a a c a c c a t a t c c a t c c a a g t a g t a t c a c t t a t t t t a g t t g a c g a a a t t g t g a a c a c g g a c t t g t g g t a t a g g t a g g

PaeR7IPspXIXhoIEagINotI 3720 T7 promoter T A A T A C G A C T C A C T A T A G G G T7 g g c g t a a t a c g a c t c a c t a t a g g g a g a g c g g c c g c c a g a t c t t c c g g a t g g c t c g a g t t t c c g c a t t a t g c t g a g t g a t a t c c c t c t c g c c g g c g g t c t a g a a g g c c t a c c g a g c t c a a a

3730 *CS t t c a g c a a g a a a g t c g t t c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.