Addgene pC4-RhE-FRB-Fis1 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pC4-RhE-FRB-Fis1


Map View

M13 fwd M13 Forward (5431 .. 5448) M13/pUC Forward (5417 .. 5439) PvuII (5361) F1ori-R (5181 .. 5200) NaeI (5159) NgoMIV (5157) DraIII (5056) F1ori-F (4969 .. 4990) pRS-marker (4746 .. 4765) pGEX 3' (4624 .. 4646) PfoI (4641) pBRforEco (4568 .. 4586) XmnI (4207) Amp-R (4146 .. 4165) BsaI (3657) AhdI (3596) PspFI (3011) BseYI (3007) pBR322ori-F (2844 .. 2863) MluI (1) CMV enhancer SnaBI (298) CMV-F (477 .. 497) LNCX (523 .. 547) BbsI (592) HSV TK 5'-UTR EcoRI (656) Kozak sequence XbaI (682) EcoNI (721) SphI (793) SpeI (967) SacII (981) PstI (992) PasI (1095) BamHI (1156) KflI (1185) HincII (1337) MfeI (1527) BglII (1876) Bglob-pA-R (1879 .. 1898) rbglobpA-R (1998 .. 2017) PflMI (2082) AbsI - AvaI - BsoBI - PaeR7I - PspXI - XhoI (2325) AvrII (2348) SfiI (2402) BspQI - SapI (2587) L4440 (2593 .. 2610) pC4-RhE-FRB-Fis1 5473 bp

Sequence View

MluI 60 CMV enhancer a c g c g t t c g a g c t c g c c c c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c t g c g c a a g c t c g a g c g g g g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g

120 CMV enhancer c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c

180 CMV enhancer g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t

240 CMV enhancer t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g

SnaBI 300 CMV enhancer c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a

360 CMV enhancer CMV promoter a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t

420 CMV promoter g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a

480 CMV promoter C G C A CMV-F t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t

540 CMV promoter A A T G G G C G G T A G G C G T G CMV-F A G C T C G T T T A G T G A A C C G LNCX a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c g t t t a g t g a a c c g t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g c a a a t c a c t t g g c

BbsI 600 T C A G A T C LNCX t c a g a t c g c c t g g a g a c g c c a t c c a c g c t g t t t t g a c c t c c a t a g a a g a c a c c g g g a c c g a g t c t a g c g g a c c t c t g c g g t a g g t g c g a c a a a a c t g g a g g t a t c t t c t g t g g c c c t g g c

EcoRI 660 HSV TK 5'-UTR a t c c a g c c t c c g g g g g a t c t t g g t g g c g t g a a a c t c c c g c a c c t c t t c g g c c a g c g a a t t t a g g t c g g a g g c c c c c t a g a a c c a c c g c a c t t t g a g g g c g t g g a g a a g c c g g t c g c t t a a

XbaI 720 2025 2030 ILWHEMWHEGL Kozak sequence FRB* MASRILWHEMWHEGL c c a g a a g c c g c c a c c a t g g c t t c t a g a a t c c t c t g g c a t g a g a t g t g g c a t g a a g g c c t g g g t c t t c g g c g g t g g t a c c g a a g a t c t t a g g a g a c c g t a c t c t a c a c c g t a c t t c c g g a c

EcoNI 780 2035 2040 2045 2050 EEASRLYFGERNVKGMFEVL FRB* EEASRLYFGERNVKGMFEVL g a a g a g g c a t c t c g t t t g t a c t t t g g g g a a a g g a a c g t g a a a g g c a t g t t t g a g g t g c t g c t t c t c c g t a g a g c a a a c a t g a a a c c c c t t t c c t t g c a c t t t c c g t a c a a a c t c c a c g a c

SphI 840 2055 2060 2065 2070 EPLHAMMERGPQTLKETSFN FRB* EPLHAMMERGPQTLKETSFN g a g c c c t t g c a t g c t a t g a t g g a a c g g g g c c c c c a g a c t c t g a a g g a a a c a t c c t t t a a t c t c g g g a a c g t a c g a t a c t a c c t t g c c c c g g g g g t c t g a g a c t t c c t t t g t a g g a a a t t a

900 2075 2080 2085 2090 QAYGRDLMEAQEWCRKYMKS FRB* QAYGRDLMEAQEWCRKYMKS c a g g c c t a t g g t c g a g a t t t a a t g g a g g c c c a a g a g t g g t g c a g g a a g t a c a t g a a a t c a g t c c g g a t a c c a g c t c t a a a t t a c c t c c g g g t t c t c a c c a c g t c c t t c a t g t a c t t t a g t

960 2095 2100 2105 2110 GNVKDLLQAWDLYYHVFRRI FRB* GNVKDLLQAWDLYYHVFRRI g g g a a t g t c a a g g a c c t c c t c c a a g c c t g g g a c c t c t a t t a t c a t g t g t t c c g a c g a a t c c c c t t a c a g t t c c t g g a g g a g g t t c g g a c c c t g g a g a t a a t a g t a c a c a a g g c t g c t t a g

PstISacIISpeI 1020 SK (in frame with FRB*) TSYVRGLLQTEPQNNQAK FRB* SKTSYVRGLLQTEPQNNQAK t c a a a g a c t a g t t a c g t c c g c g g g t t g c t g c a g a c a g a g c c c c a g a a c a a c c a g g c c a a g a g t t t c t g a t c a a t g c a g g c g c c c a a c g a c g t c t g t c t c g g g g t c t t g t t g g t c c g g t t c

1080 (in frame with FRB*) ELERLIDKAMKKDGLVGMAI ELERLIDKAMKKDGLVGMAI g a a c t g g a g c g g c t c a t t g a c a a g g c c a t g a a g a a a g a t g g a c t c g t g g g c a t g g c c a t c c t t g a c c t c g c c g a g t a a c t g t t c c g g t a c t t c t t t c t a c c t g a g c a c c c g t a c c g g t a g

PasI 1140 (in frame with FRB*) VGGMALGVAGLAGLIGLAVS VGGMALGVAGLAGLIGLAVS g t g g g a g g c a t g g c c c t g g g t g t g g c g g g a c t g g c c g g a c t c a t c g g a c t t g c t g t g t c c c a c c c t c c g t a c c g g g a c c c a c a c c g c c c t g a c c g g c c t g a g t a g c c t g a a c g a c a c a g g

KflIBamHI 1200 (in frame with FRB*) KSKS* β- globin intron KSKS* a a g t c c a a a t c c t g a g g a t c c t g a g a a c t t c a g g g t g a g t t t g g g g a c c c t t g a t t g t t c t t c a g g t t t a g g a c t c c t a g g a c t c t t g a a g t c c c a c t c a a a c c c c t g g g a a c t a a c a a g

1260 β- globin intron t t t c t t t t t c g c t a t t g t a a a a t t c a t g t t a t a t g g a g g g g g c a a a g t t t t c a g g g t g t t a a a g a a a a a g c g a t a a c a t t t t a a g t a c a a t a t a c c t c c c c c g t t t c a a a a g t c c c a c a a

1320 β- globin intron g t t t a g a a t g g g a a g a t g t c c c t t g t a t c a c c a t g g a c c c t c a t g a t a a t t t t g t t t c t t c a a a t c t t a c c c t t c t a c a g g g a a c a t a g t g g t a c c t g g g a g t a c t a t t a a a a c a a a g a a

HincII 1380 β- globin intron t c a c t t t c t a c t c t g t t g a c a a c c a t t g t c t c c t c t t a t t t t c t t t t c a t t t t c t g t a a c a g t g a a a g a t g a g a c a a c t g t t g g t a a c a g a g g a g a a t a a a a g a a a a g t a a a a g a c a t t g

1440 β- globin intron t t t t t c g t t a a a c t t t a g c t t g c a t t t g t a a c g a a t t t t t a a a t t c a c t t t t g t t t a t t t a a a a a g c a a t t t g a a a t c g a a c g t a a a c a t t g c t t a a a a a t t t a a g t g a a a a c a a a t a a a

1500 β- globin intron g t c a g a t t g t a a g t a c t t t c t c t a a t c a c t t t t t t t t c a a g g c a a t c a g g g t a t a t t a t a c a g t c t a a c a t t c a t g a a a g a g a t t a g t g a a a a a a a a g t t c c g t t a g t c c c a t a t a a t a t

MfeI 1560 β- globin intron t t g t a c t t c a g c a c a g t t t t a g a g a a c a a t t g t t a t a a t t a a a t g a t a a g g t a g a a t a t t a a c a t g a a g t c g t g t c a a a a t c t c t t g t t a a c a a t a t t a a t t t a c t a t t c c a t c t t a t a a

1620 β- globin intron t c t g c a t a t a a a t t c t g g c t g g c g t g g a a a t a t t c t t a t t g g t a g a a a c a a c t a c a t c c t a g a c g t a t a t t t a a g a c c g a c c g c a c c t t t a t a a g a a t a a c c a t c t t t g t t g a t g t a g g a

1680 β- globin intron g g t c a t c a t c c t g c c t t t c t c t t t a t g g t t a c a a t g a t a t a c a c t g t t t g a g a t g a g g a t c c a g t a g t a g g a c g g a a a g a g a a a t a c c a a t g t t a c t a t a t g t g a c a a a c t c t a c t c c t a

1740 β- globin intron a a a a t a c t c t g a g t c c a a a c c g g g c c c c t c t g c t a a c c a t g t t c a t g c c t t c t t c t t t t t t t t t a t g a g a c t c a g g t t t g g c c c g g g g a g a c g a t t g g t a c a a g t a c g g a a g a a g a a a a a

1800 β- globin intron c c t a c a g c t c c t g g g c a a c g t g c t g g t t g t t g t g c t g t c t c a t c a t t t t g g c a a a g g a t t g g a t g t c g a g g a c c c g t t g c a c g a c c a a c a a c a c g a c a g a g t a g t a a a a c c g t t t c c t a a

1860 c a c t c c t c a g g t g c a g g c t g c c t a t c a g a a g g t g g t g g c t g g t g t g g c c a a t g c c c t g g c g t g a g g a g t c c a c g t c c g a c g g a t a g t c t t c c a c c a c c g a c c a c a c c g g t t a c g g g a c c g

BglII 1920 A G A A A A A G G G A G A C G G T T T T Bglob-pA-R t c a c a a a t a c c a c t g a g a t c t t t t t c c c t c t g c c a a a a a t t a t g g g g a c a t c a t g a a g c c a g t g t t t a t g g t g a c t c t a g a a a a a g g g a g a c g g t t t t t a a t a c c c c t g t a g t a c t t c g g

1980 β- globin poly(A) signal c c t t g a g c a t c t g a c t t c t g g c t a a t a a a g g a a a t t t a t t t t c a t t g c a a t a g t g t g t t g g g a a c t c g t a g a c t g a a g a c c g a t t a t t t c c t t t a a a t a a a a g t a a c g t t a t c a c a c a a c

2040 β- globin poly(A) signal G T G A G C C T T C C T G T A T A C C C rbglobpA-R g a a t t t t t t g t g t c t c t c a c t c g g a a g g a c a t a t g g g a g g g c a a a t c a t t t a a a a c a t c a c t t a a a a a a c a c a g a g a g t g a g c c t t c c t g t a t a c c c t c c c g t t t a g t a a a t t t t g t a g t

PflMI 2100 g a a t g a g t a t t t g g t t t a g a g t t t g g c a a c a t a t g c c a t a t g c t g g c t g c c a t g a a c a a a c t t a c t c a t a a a c c a a a t c t c a a a c c g t t g t a t a c g g t a t a c g a c c g a c g g t a c t t g t t t

2160 g g t g g c t a t a a a g a g g t c a t c a g t a t a t g a a a c a g c c c c c t g c t g t c c a t t c c t t a t t c c c c a c c g a t a t t t c t c c a g t a g t c a t a t a c t t t g t c g g g g g a c g a c a g g t a a g g a a t a a g g

2220 a t a g a a a a g c c t t g a c t t g a g g t t a g a t t t t t t t t a t a t t t t g t t t t g t g t t a t t t t t t t t a t c t t t t c g g a a c t g a a c t c c a a t c t a a a a a a a a t a t a a a a c a a a a c a c a a t a a a a a a a

2280 c t t t a a c a t c c c t a a a a t t t t c c t t a c a t g t t t t a c t a g c c a g a t t t t t c c t c c t c t c c t g a a a t t g t a g g g a t t t t a a a a g g a a t g t a c a a a a t g a t c g g t c t a a a a a g g a g g a g a g g a

PaeR7IBsoBIPspXIXhoIAvaIAbsI 2340 g a c t a c t c c c a g t c a t a g c t g t c c c t c t t c t c t t a t g a a g a t c c c t c g a g g a g c t t t t t g c t g a t g a g g g t c a g t a t c g a c a g g g a g a a g a g a a t a c t t c t a g g g a g c t c c t c g a a a a a c

AvrII 2400 SV40 ori c a a a a g c c c t a g g c c t c c a a a a a a g c c t c t t c a c t a c t t c t g g a a t a g c t c a g a g g c c g a g t t t t c g g g a t c c g g a g g t t t t t t c g g a g a a g t g a t g a a g a c c t t a t c g a g t c t c c g g c t

SfiI 2460 SV40 ori g g c g g c c t c g g c c t c t g c a t a a a t a a a a a a a a t t a g t c a g c c a t g g g g c g g a g a a t g g g c c c g c c g g a g c c g g a g a c g t a t t t a t t t t t t t t a a t c a g t c g g t a c c c c g c c t c t t a c c c g

2520 SV40 ori g g a a c t g g g c g g a g t t a g g g g c g g g a t g g g c g g a g t t a g g g g c g g g a c t a t g g t t g c t g a c c t t g a c c c g c c t c a a t c c c c g c c c t a c c c g c c t c a a t c c c c g c c c t g a t a c c a a c g a c t

2580 c t a a t t g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g g a t t a a c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c

BspQISapI 2640 G A G C G A G T G A C T G A G C G A L4440 c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a

2700 a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t

2760 g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g

2820 ori g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c

2880 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a

2940 ori g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c

3000 ori a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c

BseYIPspFI 3060 ori c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c

3120 ori t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g

3180 ori t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c

3240 ori g c c t a a c t a c g g c t a c a c t a g a a g g a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t c g g a t t g a t g c c g a t g t g a t c t t c c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a

3300 ori t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c

3360 ori t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g

3420 t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a

3480 g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a

3540 *WHKIL t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c

AhdI 3600 SAGIEAIQRNREDMTAQSGT t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a

BsaI 3660 TYIVVIRSPKGDPGLAAIIG c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g

3720 RSGREGAGSKDAIFWGAPLA g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g

3780 SRLLPGAVKDAEMWDILQQR c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c

3840 SALTLLEGTLLKRLTTAMAV g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g

3900 PMTTDREDNPIAENLEPEWR a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c

3960 DLRTVHDGMNHLFATLEKPG a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g

4020 GITTLLLNAATNDSMTIAAS t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a

4080 CLERVTMGDTLHKETVPSYE g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g

4140 VLDNQSYHIRRGLQEQGADI a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a

4200 A T A A T A C C G C G C C A C A T A G C Amp-R RSLVAGCLLVKFTSMMAVEV a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g c c a c c t c c a c t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c g g t g g a g g t g

XmnI 4260 QFREEPRFSELIKGSNLDLE c t g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g

4320 IYGVRAGLQDEADKVKVLTE g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g

4380 PHAFVPLCFAAFFPILAVRF t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t

4440 AmpR promoter HQISM a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c

4500 AmpR promoter t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c

4560 c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g

4620 A A T A G G C G T A T C A C G A G G C pBRforEco c t a t a a a a a t a g g c g t a t c a c g a g g c c c t t t c g t c t c g c g c g t t t c g g t g a t g a c g g t g a g a t a t t t t t a t c c g c a t a g t g c t c c g g g a a a g c a g a g c g c g c a a a g c c a c t a c t g c c a c t

PfoI 4680 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a c a g c t t g t c t g t a a g c g g a t g c c g g t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t g t c g a a c a g a c a t t c g c c t a c g g c c

4740 g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g t t g g c g g g t g t c g g g g c t g g c t t a a c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c a a c c g c c c a c a g c c c c g a c c g a a t t

4800 C G G C A T C A G A G C A G A T T G T A pRS-marker c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a c c a t a t g c g g t g t g a a a t a c c g c a g a t a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g t g g t a t a c g c c a c a c t t t a t g g c g t

4860 f1 ori c a g a t g c g t a a g g a g a a a a t a c c g c a t c a g g a a a t t g t a a a c g t t a a t a t t t t g t t a a a a g t c t a c g c a t t c c t c t t t t a t g g c g t a g t c c t t t a a c a t t t g c a a t t a t a a a a c a a t t t t

4920 f1 ori t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t

4980 f1 ori G G T C A A A C C T T G F1ori-F a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g

5040 f1 ori T T C T C A G G T G F1ori-F a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c

DraIII 5100 f1 ori g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a

NgoMIVNaeI 5160 f1 ori a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c

5220 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t

5280 f1 ori a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c

5340 f1 ori g g c g c g t c g c g c c a t t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g c c g c g c a g c g c g g t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c

PvuII 5400 g c c t c t t c g c t a t t a c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g c g g a g a a g c g a t a a t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c

5460 M13 fwd T7 promoter C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward g t a a c g c c a g g g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t g a a t t g t a a t a c c a t t g c g g t c c c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a c t t a a c a t t a t g

5473 T7 promoter g a c t c a c t a t a g g c t g a g t g a t a t c c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.