Addgene pFETCh_RAD21 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pFETCh_RAD21


Map View

DraIII (7942) pENTR-R (7832 .. 7851) Kan-R (7748 .. 7767) AsiSI (7410) PflMI (7148) M13/pUC Forward (6799 .. 6821) BstXI (6776) AflII (6309) BsgI (6096) ScaI (6090) BstEII (5644) Bsu36I (5617) BsaBI (5455) SpeI (5169) BstBI (5129) Neo-F (4997 .. 5016) PflFI - Tth111I (4579) PluTI (4464) SfoI (4462) NarI (4461) KasI (4460) Neo-R (4387 .. 4406) FLAG FLAG ApaLI (384) pBR322ori-F (543 .. 562) L4440 (796 .. 813) AclI (867) CAP binding site M13/pUC Reverse (1192 .. 1214) lac operator M13 Reverse (1211 .. 1227) M13 rev SalI (1258) XbaI (1264) Acc65I (1279) KpnI (1283) CMV-F (1557 .. 1577) LNCX (1603 .. 1627) MluI (1728) BsiWI (1778) XcmI (1903) BspEI (2326) PshAI (2753) AleI (2912) EcoRI (3725) FLAG pFETCh_RAD21 8166 bp

Sequence View

5ʹ 3ʹ 60 ori t c g a c g g a g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t a g c t g c c t c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a

120 ori c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c

180 ori g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t

240 ori g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g

300 ori t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a

360 ori g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c

ApaLI 420 ori c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g

480 ori g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c

540 ori g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t

600 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a

660 ori c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g

720 t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g

780 c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g

840 A G C G A G T C A G T G A G C G A G L4440 c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a a g c t c g c a c a t t c a g c g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t t c g a g c g t g t a a g t c g

AclI 900 a g c g t t t t t c a g c g c g t t t t c g a t c a a c g t t t c a a t g t t g g t a t c a a c a c c a g g t t t a a c t c g c a a a a a g t c g c g c a a a a g c t a g t t g c a a a g t t a c a a c c a t a g t t g t g g t c c a a a t t g

960 t t t g a a c t t a t c g g c a c t g a c g g t t a c t g a t t t t g a a c t t t t g c t t t g c c a c g g a a c g g t a a a c t t g a a t a g c c g t g a c t g c c a a t g a c t a a a a c t t g a a a a c g a a a c g g t g c c t t g c c a

1020 c t g c g t t g t c g g g a a g a t g c g t g a t c t g a t c c t t c a a c t c a g c a a a a g t t c c c a a t a c g c g a c g c a a c a g c c c t t c t a c g c a c t a g a c t a g g a a g t t g a g t c g t t t t c a a g g g t t a t g c g

1080 a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g

1140 CAP binding site g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t

1200 -35 -10 lac promoter lac operator A G C G G A T A A M13/pUC Reverse c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t

SalI 1260 lac operator M13 rev C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c t t g c a t g c c t g c a g g t c g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g a a c g t a c g g a c g t c c a g

Acc65IKpnIXbaI 1320 g a c t c t a g a g g a t c c c c g g g t a c c g a g c t c g a a t t a g c t t g c a t g c c t g c a g g t c a a t t c c t g a g a t c t c c t a g g g g c c c a t g g c t c g a g c t t a a t c g a a c g t a c g g a c g t c c a g t t a a g

1380 c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c

1440 CMV promoter t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a

1500 CMV promoter a g c g g t t t g a c t c a c g g g g a t t t c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t c g c c a a a c t g a g t g c c c c t a a a g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a

1560 CMV promoter C G C A CMV-F t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t

1620 CMV promoter A A T G G G C G G T A G G C G T G CMV-F A G C T C G T T T A G T G A A C C G LNCX MGGRRVRWEVYISRARLVNR a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c g t t t a g t g a a c c g t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g c a a a t c a c t t g g c

1680 T C A G A T C LNCX QIAWRRHPRCFDLHRRHRDR t c a g a t c g c c t g g a g a c g c c a t c c a c g c t g t t t t g a c c t c c a t a g g a g a c a c c g g g a c c g a g t c t a g c g g a c c t c t g c g g t a g g t g c g a c a a a a c t g g a g g t a t c c t c t g t g g c c c t g g c

MluI 1740 1 5 10 MASYPCHQHASAF HSV TK SSLGIPTMASYPCHQHASAF a t c c a g c c t g g g g a t c c c g a c c a t g g c t t c g t a c c c c t g c c a t c a a c a c g c g t c t g c g t t t a g g t c g g a c c c c t a g g g c t g g t a c c g a a g c a t g g g g a c g g t a g t t g t g c g c a g a c g c a a

BsiWI 1800 15 20 25 30 DQAARSRGHSNRRTALRPRR HSV TK DQAARSRGHSNRRTALRPRR c g a c c a g g c t g c g c g t t c t c g c g g c c a t a g c a a c c g a c g t a c g g c g t t g c g c c c t c g c c g g c t g g t c c g a c g c g c a a g a g c g c c g g t a t c g t t g g c t g c a t g c c g c a a c g c g g g a g c g g c

1860 35 40 45 50 QQEATEVRLEQKMPTLLRVY HSV TK QQEATEVRLEQKMPTLLRVY g c a g c a a g a a g c c a c g g a a g t c c g c c t g g a g c a g a a a a t g c c c a c g c t a c t g c g g g t t t a c g t c g t t c t t c g g t g c c t t c a g g c g g a c c t c g t c t t t t a c g g g t g c g a t g a c g c c c a a a t

XcmI 1920 55 60 65 70 IDGPHGMGKTTTTQLLVALG HSV TK IDGPHGMGKTTTTQLLVALG t a t a g a c g g t c c t c a c g g g a t g g g g a a a a c c a c c a c c a c g c a a c t g c t g g t g g c c c t g g g a t a t c t g c c a g g a g t g c c c t a c c c c t t t t g g t g g t g g t g c g t t g a c g a c c a c c g g g a c c c

1980 75 80 85 90 SRDDIVYVPEPMTYWQVLGA HSV TK SRDDIVYVPEPMTYWQVLGA t t c g c g c g a c g a t a t c g t c t a c g t a c c c g a g c c g a t g a c t t a c t g g c a g g t g c t g g g g g c a a g c g c g c t g c t a t a g c a g a t g c a t g g g c t c g g c t a c t g a a t g a c c g t c c a c g a c c c c c g

2040 95 100 105 110 SETIANIYTTQHRLDQGEIS HSV TK SETIANIYTTQHRLDQGEIS t t c c g a g a c a a t c g c g a a c a t c t a c a c c a c a c a a c a c c g c c t c g a c c a g g g t g a g a t a t c a a g g c t c t g t t a g c g c t t g t a g a t g t g g t g t g t t g t g g c g g a g c t g g t c c c a c t c t a t a g

2100 115 120 125 130 AGDAAVVMTSAQITMGMPYA HSV TK AGDAAVVMTSAQITMGMPYA g g c c g g g g a c g c g g c g g t g g t a a t g a c a a g c g c c c a g a t a a c a a t g g g c a t g c c t t a t g c c c g g c c c c t g c g c c g c c a c c a t t a c t g t t c g c g g g t c t a t t g t t a c c c g t a c g g a a t a c g

2160 135 140 145 150 VTDAVLAPHIGGEAGSSHAP HSV TK VTDAVLAPHIGGEAGSSHAP c g t g a c c g a c g c c g t t c t g g c t c c t c a t a t c g g g g g g g a g g c t g g g a g c t c a c a t g c c c c g c a c t g g c t g c g g c a a g a c c g a g g a g t a t a g c c c c c c c t c c g a c c c t c g a g t g t a c g g g g

2220 155 160 165 170 PPALTLIFDRHPIAALLCYP HSV TK PPALTLIFDRHPIAALLCYP g c c c c c g g c c c t c a c c c t c a t c t t c g a c c g c c a t c c c a t c g c c g c c c t c c t g t g c t a c c c c g g g g g c c g g g a g t g g g a g t a g a a g c t g g c g g t a g g g t a g c g g c g g g a g g a c a c g a t g g g

2280 175 180 185 190 AARYLMGSMTPQAVLAFVAL HSV TK AARYLMGSMTPQAVLAFVAL g g c c g c g c g a t a c c t t a t g g g c a g c a t g a c c c c c c a g g c c g t g c t g g c g t t c g t g g c c c t c c g g c g c g c t a t g g a a t a c c c g t c g t a c t g g g g g g t c c g g c a c g a c c g c a a g c a c c g g g a

BspEI 2340 195 200 205 210 IPPTLPGTNIVLGALPEDRH HSV TK IPPTLPGTNIVLGALPEDRH c a t c c c g c c g a c c t t g c c c g g c a c a a a c a t c g t g t t g g g g g c c c t t c c g g a g g a c a g a c a g t a g g g c g g c t g g a a c g g g c c g t g t t t g t a g c a c a a c c c c c g g g a a g g c c t c c t g t c t g t

2400 215 220 225 230 IDRLAKRQRPGERLDLAMLA HSV TK IDRLAKRQRPGERLDLAMLA c a t c g a c c g c c t g g c c a a a c g c c a g c g c c c c g g c g a g c g g c t t g a c c t g g c t a t g c t g g c g t a g c t g g c g g a c c g g t t t g c g g t c g c g g g g c c g c t c g c c g a a c t g g a c c g a t a c g a c c g

2460 235 240 245 250 AIRRVYGLLANTVRYLQGGG HSV TK AIRRVYGLLANTVRYLQGGG c g c g a t t c g c c g c g t t t a c g g g c t g c t t g c c a a t a c g g t g c g g t a t c t g c a g g g c g g c g g g c g c t a a g c g g c g c a a a t g c c c g a c g a a c g g t t a t g c c a c g c c a t a g a c g t c c c g c c g c c

2520 255 260 265 270 SWREDWGQLSGTAVPPQGAE HSV TK SWREDWGQLSGTAVPPQGAE g t c g t g g c g g g a g g a t t g g g g a c a g c t t t c g g g g a c g g c c g t g c c g c c c c a g g g t g c c g a c a g c a c c g c c c t c c t a a c c c c t g t c g a a a g c c c c t g c c g g c a c g g c g g g g t c c c a c g g c t

2580 275 280 285 290 PQSNAGPRPHIGDTLFTLFR HSV TK PQSNAGPRPHIGDTLFTLFR g c c c c a g a g c a a c g c g g g c c c a c g a c c c c a t a t c g g g g a c a c g t t a t t t a c c c t g t t t c g c g g g g t c t c g t t g c g c c c g g g t g c t g g g g t a t a g c c c c t g t g c a a t a a a t g g g a c a a a g c

2640 295 300 305 310 APELLAPNGDLYNVFAWALD HSV TK APELLAPNGDLYNVFAWALD g g c c c c c g a g t t g c t g g c c c c c a a c g g c g a c c t g t a c a a c g t g t t t g c c t g g g c c t t g g a c c g g g g g c t c a a c g a c c g g g g g t t g c c g c t g g a c a t g t t g c a c a a a c g g a c c c g g a a c c t

2700 315 320 325 330 VLAKRLRPMHVFILDYDQSP HSV TK VLAKRLRPMHVFILDYDQSP c g t c t t g g c c a a a c g c c t c c g t c c c a t g c a c g t c t t t a t c c t g g a t t a c g a c c a a t c g c c g c a g a a c c g g t t t g c g g a g g c a g g g t a c g t g c a g a a a t a g g a c c t a a t g c t g g t t a g c g g

PshAI 2760 335 340 345 350 AGCRDALLQLTSGMVQTHVT HSV TK AGCRDALLQLTSGMVQTHVT c g c c g g c t g c c g g g a c g c c c t g c t g c a a c t t a c c t c c g g g a t g g t c c a g a c c c a c g t c a c g c g g c c g a c g g c c c t g c g g g a c g a c g t t g a a t g g a g g c c c t a c c a g g t c t g g g t g c a g t g

2820 355 360 365 370 TPGSIPTICDLARTFAREMG HSV TK TPGSIPTICDLARTFAREMG c a c c c c c g g c t c c a t a c c g a c g a t c t g c g a c c t g g c g c g c a c g t t t g c c c g g g a g a t g g g g t g g g g g c c g a g g t a t g g c t g c t a g a c g c t g g a c c g c g c g t g c a a a c g g g c c c t c t a c c c

2880 375 EAN* HSV TK EAN* g g a g g c t a a c t g a g g a t c c c a c g t c a c t a t t g t a t a c t c t a t a t t a t a c t c t a t g t t a t a c c t c c g a t t g a c t c c t a g g g t g c a g t g a t a a c a t a t g a g a t a t a a t a t g a g a t a c a a t a t

AleI 2940 c t c t g t a a t c c t a c t c a a t a a a c g t g t c a c g c c t g t g a a a c c g t a c t a a g t c t c c c g t g t g a g a c a t t a g g a t g a g t t a t t t g c a c a g t g c g g a c a c t t t g g c a t g a t t c a g a g g g c a c a

3000 a t t c t t a t c a c c a t c a g g t g a c a t c c t c g c c c a g g c t g t c a a t c a t g c c g g t a t c g a t t c t a a g a a t a g t g g t a g t c c a c t g t a g g a g c g g g t c c g a c a g t t a g t a c g g c c a t a g c t a a g

3060 c a g t a g c a c c g g c c c c a c g c t g a c a a c c c a c t c t t g c a g c g t t a g c a g c g c c c c t c t t a a g t c a t c g t g g c c g g g g t g c g a c t g t t g g g t g a g a a c g t c g c a a t c g t c g c g g g g a g a a t t

3120 c a a g c c g a c c c c c a c c a g c g t c g c g g t t a c t a a c a c t c c t c t c c c c g a c c t g c a g c c c a g g t t c g g c t g g g g g t g g t c g c a g c g c c a a t g a t t g t g a g g a g a g g g g c t g g a c g t c g g g t c

3180 c t t c c c c g a c c t g c a g c c c a g c t g c a a a a t g c c a a g c a a g t t g c t c a a g g t t t c t t a a a a g a a g g g g c t g g a c g t c g g g t c g a c g t t t t a c g g t t c g t t c a a c g a g t t c c a a a g a a t t t t

3240 t a g g a c a g t t g a c a t g t g t t t a t t a a t t t t t t a c t t t a a a a a c t a t a a g t g c t a g a t t t t a t c c t g t c a a c t g t a c a c a a a t a a t t a a a a a a t g a a a t t t t t g a t a t t c a c g a t c t a a a a

3300 a c t a c t a t a t t a c a t t a g c t g t c t a a a g a a a t t g g t t a t t t t a a t a a t a c a t g t c a g a a c t g a t g a t a t a a t g t a a t c g a c a g a t t t c t t t a a c c a a t a a a a t t a t t a t g t a c a g t c t t g

3360 a a t t t t g c t g g t t t c t t g g a c t t t c t t a t t t g g a t t t t a g a t g t a c c a t a a c a t a c t t t g t t a a a a c g a c c a a a g a a c c t g a a a g a a t a a a c c t a a a a t c t a c a t g g t a t t g t a t g a a a c

3420 g c c t t t t a c c c t c t t g a t t a a c a t g a g a a t c a c a t a c t a t t t a a a a a a g t g c a a t c t t t g c g g a a a a t g g g a g a a c t a a t t g t a c t c t t a g t g t a t g a t a a a t t t t t t c a c g t t a g a a a c

3480 t c c t a a g c a g a a t a t a t a g t t t g t t t t t t c c a g t a a c c a a a a t t a g c a t c t g t g t t a g t c a g g a t t c g t c t t a t a t a t c a a a c a a a a a a g g t c a t t g g t t t t a a t c g t a g a c a c a a t c a g

3540 t g t t t t g t g t t g c t c t a a a g g a a t a c c t g a g t c t g g g t a a t t t a t a a a g a a g t t t a t t t g a c a a a a c a c a a c g a g a t t t c c t t a t g g a c t c a g a c c c a t t a a a t a t t t c t t c a a a t a a a c

3600 g c t c a c a g t t c t g c a g a c t a t a c a a g a a g c a a a g c a c c a g c a t c t g c t t c t g g t g a g g c c c g a g t g t c a a g a c g t c t g a t a t g t t c t t c g t t t c g t g g t c g t a g a c g a a g a c c a c t c c g g

3660 t c a g a a a g c t t a t a g t c a t g g t g g a a g g c a a a g g g g g a g c a g g t g t g t c a c a t g g c a a g a a g t c t t t c g a a t a t c a g t a c c a c c t t c c g t t t c c c c c t c g t c c a c a c a g t g t a c c g t t c t

3720 g a g g g c a t g a g a g a g a t g g c t a g g c g c t t t t a a a c a a g c a g c t c t t g t g t t a a c t a a c g a c t c c c g t a c t c t c t c t a c c g a t c c g c g a a a a t t t g t t c g t c g a g a a c a c a a t t g a t t g c t

EcoRI 3780 g c a g g a a t t c a c t g a t t g c c a c a g g g a g t g a t t g a t a a g g g a t c c a c c a c c a c c c c c a t c c g t c c t t a a g t g a c t a a c g g t g t c c c t c a c t a a c t a t t c c c t a g g t g g t g g t g g g g g t a g

3840 c c c a c c c c a t g a c a c a a a c a c c t c a c t a g g c t c c a t c t c t a a c a c t g g g a a a t t a c g t t t g g g t g g g g t a c t g t g t t t g t g g a g t g a t c c g a g g t a g a g a t t g t g a c c c t t t a a t g c a a a

3900 c a g c a t g a g a t t t g g a g g g g a c a a a t a t t c a a a c t g t a t c a g c a t c c t c a c t g a a t t a c t g t c g t a c t c t a a a c c t c c c c t g t t t a t a a g t t t g a c a t a g t c g t a g g a g t g a c t t a a t g a

3960 a c t t t a t t a t a c a g a t t a a g t t a c c c t t t t t t g g t t t t a t t t c a g c g t g c t c t t g c t a a a t g a a a t a a t a t g t c t a a t t c a a t g g g a a a a a a c c a a a a t a a a g t c g c a c g a g a a c g a t t t

4020 a c t g g a g c t g a a t c t a t c a g t t t g c t t g a g t t a t g t c g a a a t a c g a a c a g a a a a c a a g c t t g a c c t c g a c t t a g a t a g t c a a a c g a a c t c a a t a c a g c t t t a t g c t t g t c t t t t g t t c g a

4080 g c c g c a a a g t t c t a c a g c t t c t t g g t t c t t a a a a a g c a g c a a g c t a t t g a g c t g a c a c a g c g g c g t t t c a a g a t g t c g a a g a a c c a a g a a t t t t t c g t c g t t c g a t a a c t c g a c t g t g t c

4140 g a a g a a c c g t a c a g t g a c a t c a t c g c a a c a c c t g g a c c a a g g t t c c a t a t t a t a g g g a g c c t t c t t g g c a t g t c a c t g t a g t a g c g t t g t g g a c c t g g t t c c a a g g t a t a a t a t c c c t c g

4200 1 5 DYKDDDDK G 1 D FLAG FLAG g g a g g a g g t t c c g g t g g a g g t g g t t c t g g a g a t t a c a a g g a t g a c g a c g a t a a g g g c g a t c c t c c t c c a a g g c c a c c t c c a c c a a g a c c t c t a a t g t t c c t a c t g c t g c t a t t c c c g c t a

4260 5 YKDDDDK G 1 5 DYKDDDDK VSGS FLAG FLAG t a c a a g g a t g a c g a c g a t a a g g g a g a t t a c a a g g a t g a c g a c g a t a a g g t t t c a g g a a g c a t g t t c c t a c t g c t g c t a t t c c c t c t a a t g t t c c t a c t g c t g c t a t t c c a a a g t c c t t c g

4320 G 1 5 10 15 ATNFSLLKQAGDVEENPGP P2A g g a g c t a c t a a c t t c a g c c t g c t g a a g c a g g c t g g a g a c g t g g a g g a g a a c c c t g g a c c t c c t c g a t g a t t g a a g t c g g a c g a c t t c g t c c g a c c t c t g c a c c t c c t c t t g g g a c c t g g a

4380 GSFR 1 5 10 15 MIEQDGLHAGSPAAWV NeoR/KanR MIEQDGLHAGSPAAWV g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c

4440 20 25 30 35 ERLFGYDWAQQTIGCSDAAV NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R ERLFGYDWAQQTIGCSDAAV g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c

KasINarISfoIPluTI 4500 40 45 50 55 FRLSAQGRPVLFVKTDLSGA NeoR/KanR FRLSAQGRPVLFVKTDLSGA t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g

4560 60 65 70 75 LNELQDEAARLSWLATTGVP NeoR/KanR LNELQDEAARLSWLATTGVP c t g a a t g a a c t g c a g g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t g a c t t a c t t g a c g t c c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a

Tth111IPflFI 4620 80 85 90 95 CAAVLDVVTEAGRDWLLLGE NeoR/KanR CAAVLDVVTEAGRDWLLLGE t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t

4680 100 105 110 115 VPGQDLLSSHLAPAEKVSIM NeoR/KanR VPGQDLLSSHLAPAEKVSIM *RVKSRGLFYGDH g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c

4740 120 125 130 135 ADAMRRLHTLDPATCPFDHQ NeoR/KanR ADAMRRLHTLDPATCPFDHQ SICHPPQMRKIRSGAWEVVL g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t

4800 140 145 150 155 AKHRIERARTRMEAGLVDQD NeoR/KanR AKHRIERARTRMEAGLVDQD RFMADLSCTSPHFGTKDILI g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a

4860 160 165 170 175 DLDEEHQGLAPAELFARLKA NeoR/KanR DLDEEHQGLAPAELFARLKA IQVFLMLPERWGFQEGPELR g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c

4920 180 185 190 195 RMPDGEDLVVTHGDACLPNI NeoR/KanR RMPDGEDLVVTHGDACLPNI AHGVALIEDHGMAIGAQRID c g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c g c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g

4980 200 205 210 215 MVENGRFSGFIDCGRLGVAD NeoR/KanR MVENGRFSGFIDCGRLGVAD HHFIAAKRSEDVTAPQTHGV a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c c g a c t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g g c t g

5040 220 225 230 235 RYQDIALATRDIAEELGGEW NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F RYQDIALATRDIAEELGGEW AILVYRQSGTINSFLKAAFP c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c

5100 240 245 250 255 ADRFLVLYGIAAPDSQRIAF NeoR/KanR ADRFLVLYGIAAPDSQRIAF SVAEEHKVTDGSGIRLADGE g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g

BstBI 5160 260 265 YRLLDEFF* NeoR/KanR YRLLDEFF* IAKKVLEESEFM t a t c g c c t t c t t g a c g a g t t c t t c t g a t t c g a a c a t c a g c t a g a a g c a t t a t a g c t a g t g a t a g c g g a a g a a c t g c t c a a g a a g a c t a a g c t t g t a g t c g a t c t t c g t a a t a t c g a t c a c

SpeI 5220 t t t g a t t c a c t a g t g c t t a c a a a t t g c c c c c a t g t g t a g g g g a c a c a g a a c c c t t t g a g a a a a c t a a g t g a t c a c g a a t g t t t a a c g g g g g t a c a c a t c c c c t g t g t c t t g g g a a a c t c t

5280 a a a c t t a g a t t t t t g t c t g t a c a a a g t c t t t g c c t t t t t c c t t c t t c a t t t t t t t c c a g t t t t g a a t c t a a a a a c a g a c a t g t t t c a g a a a c g g a a a a a g g a a g a a g t a a a a a a a g g t c a

5340 a c a t t a a a t t t g t c a a t t t c a t c t t t g a g g g a a a c t g a t t a g a t g g g t t g t g t t t g t g t t t g t a a t t t a a a c a g t t a a a g t a g a a a c t c c c t t t g a c t a a t c t a c c c a a c a c a a a c a c a a

5400 c t g a t g g a g a a a a c a g c a c c c c a a g g a c t c a g a a g a t g a t t t t a a c a g t t c a g a a c a g a t g a c t a c c t c t t t t g t c g t g g g g t t c c t g a g t c t t c t a c t a a a a t t g t c a a g t c t t g t c t a

BsaBI 5460 g t g t g c a a t a t t g g t g c a t g t a a t a a t g t t g a g t g g c a g t c a a a a g t c a t g a t t t t t a t c c a c a c g t t a t a a c c a c g t a c a t t a t t a c a a c t c a c c g t c a g t t t t c a g t a c t a a a a a t a g

5520 t t a g t t c t t c a t t a c t g c a t t g a a a a g g a a a a c c t g t c t g a g a a a a t g c c t g a c a g t t t a a a t c a a g a a g t a a t g a c g t a a c t t t t c c t t t t g g a c a g a c t c t t t t a c g g a c t g t c a a a t

5580 a t t t a a a a c t a t g g t g t a a g t c t t t g a c a a g a a a a a a a a a c a a a c a a a c a c t t c t t t c c a t a a a t t t t g a t a c c a c a t t c a g a a a c t g t t c t t t t t t t t t g t t t g t t t g t g a a g a a a g g t

Bsu36I 5640 t c a g t a a c a c t g g c a a t c t t c c t g t t a a c c a c t c t c c t t a g g g a t g g t a t c t g a a a c a a c a g t c a t t g t g a c c g t t a g a a g g a c a a t t g g t g a g a g g a a t c c c t a c c a t a g a c t t t g t t g

BstEII 5700 a a t g g t c a c c c t c t t g a g a t t c g t t t t a a g t g t a a t t c c a t a a t g a g c a g a g g t g t a c g c t t a c c a g t g g g a g a a c t c t a a g c a a a a t t c a c a t t a a g g t a t t a c t c g t c t c c a c a t g c g

5760 g a a a t t g t g t t a t g a c t g a t a g c c t t c a g c t a c a a a a a g a t a g g a c t g a c c t g g t t t a a a c t t t a a c a c a a t a c t g a c t a t c g g a a g t c g a t g t t t t t c t a t c c t g a c t g g a c c a a a t t t

5820 g t g t t c t a t t t t g t a a a t c a t t c c a t t t g a g t c t t t c t g a t g a a c t t g g c t a t a c t g a a a c a c a a g a t a a a a c a t t t a g t a a g g t a a a c t c a g a a a g a c t a c t t g a a c c g a t a t g a c t t t

5880 t c t g t t a t t t t a g t g a g g c t c c a a a a t g a g c a a a g c t a g g c c t g a t t a g a g t a g a g t g a c a g a c a a t a a a a t c a c t c c g a g g t t t t a c t c g t t t c g a t c c g g a c t a a t c t c a t c t c a c t g

5940 t a t t a a a a a a c a t a a c t t t c t a g g a g c t a t a a a t c a a a g t t t t a a a a a g a t g t t t g g a t a a t a a t t t t t t g t a t t g a a a g a t c c t c g a t a t t t a g t t t c a a a a t t t t t c t a c a a a c c t a t

6000 t a t t t g a g t a t t c c g a t c a t g a a a a c a g a a a t t g c c c t g c c t a c t a c a a g g a c a g a c t g a a t a a a c t c a t a a g g c t a g t a c t t t t g t c t t t a a c g g g a c g g a t g a t g t t c c t g t c t g a c t

6060 t g g g a a a t t a t g c a c c t g g t c a a c t t a g c t t t t a a g c a g a c g a t g c t g t a a a a a c t a a c g a c c c t t t a a t a c g t g g a c c a g t t g a a t c g a a a a t t c g t c t g c t a c g a c a t t t t t g a t t g c

BsgIScaI 6120 g c t t c t c t g a t a t t t a t t g t a a g t t t t a g t a c t g a t c t c c t t t t c c a g t g c t g c a c a c t c c g a a g a g a c t a t a a a t a a c a t t c a a a a t c a t g a c t a g a g g a a a a g g t c a c g a c g t g t g a g

6180 c t g g t t t g g a a c t t t a a t a g c g t t g c a a c g a a a t c c t a t a t c c a g t t t c c t g t a a t t t a a g a c c a a a c c t t g a a a t t a t c g c a a c g t t g c t t t a g g a t a t a g g t c a a a g g a c a t t a a a t t

6240 t t g a a g a a a a a t a c a t c c a a a t a a a g a c t t t a t t a t t a a c a g a c c a g a t a g c a t c a g a a a a a c t t c t t t t t a t g t a g g t t t a t t t c t g a a a t a a t a a t t g t c t g g t c t a t c g t a g t c t t t

6300 t c a t g t g a c t g t t a t g a t t a t c a g a a t g t c t t a a c t t t t t a g g g c a a a g t t a a c a c t g a a a g t a c a c t g a c a a t a c t a a t a g t c t t a c a g a a t t g a a a a a t c c c g t t t c a a t t g t g a c t t

AflII 6360 a g t t c t a g c t t a a g t g t t g a a a c t t t t g t g g g a a a a a a a a a t c a c t t t t g a a a c t c a g a c t c a a g a t c g a a t t c a c a a c t t t g a a a a c a c c c t t t t t t t t t a g t g a a a a c t t t g a g t c t g

6420 t t c a g t g t a t a c c c a a t a a t t t a a a a t t a t g t g a a a t g t t t t a a a t t t g t g a a c t c g t a a a a g t c a c a t a t g g g t t a t t a a a t t t t a a t a c a c t t t a c a a a a t t t a a a c a c t t g a g c a t t

6480 t t a c t g t t t t a a t g a t t c a g t t t c t t c a g a g t g g t a a t t g t a t a a a a t t g c t a t t g c a g c a a t g a c a a a a t t a c t a a g t c a a a g a a g t c t c a c c a t t a a c a t a t t t t a a c g a t a a c g t c g

6540 t t t a c a t t c a a t a t g a t g t g c c t g t a a a c c a a g g a g t t t t c c c c g t t t g t a a a a a g a c a t a a a t g t a a g t t a t a c t a c a c g g a c a t t t g g t t c c t c a a a a g g g g c a a a c a t t t t t c t g t a

6600 t g t a g a t a a t t g a a t g t t t g a t t t t a g a a a g g t c a t t a g t t t c t t g t t a c a c a t t t t g t t a c a t c t a t t a a c t t a c a a a c t a a a a t c t t t c c a g t a a t c a a a g a a c a a t g t g t a a a a c a a

6660 a g t c t g g t t t t t g t t g c t t a t c g g g t t t a a t a t t g t t c t t g a a a a t a g t t g a t g c t a t g t t c a g a c c a a a a a c a a c g a a t a g c c c a a a t t a t a a c a a g a a c t t t t a t c a a c t a c g a t a c a

6720 t a t g t a t a a c t t t t c t a a t a a a a g t t g t g t t a t a a g c t g t a c t t t t t t g a g t a t t c a t t a a t a c a t a t t g a a a a g a t t a t t t t c a a c a c a a t a t t c g a c a t g a a a a a a c t c a t a a g t a a t

BstXI 6780 t t c a g t t g g t g g t g g t c c t t g g a a a g t c c t c t c c a c t g a c t g t a g c c t c c a a t t c a c t g g a a g t c a a c c a c c a c c a g g a a c c t t t c a g g a g a g g t g a c t g a c a t c g g a g g t t a a g t g a c c

6840 M13 fwd G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward c c g t c g t t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a c c c a g g c a g c a a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t g g g t

6900 a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c g g g c

6960 c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g c g a t t t a t t c a a c a a g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c g c t a a a t a a g t t g t t

7020 a g c c g c c g t c c c g t c a a g t c a g c g t a a t g c t c t g c c a g t g t t a c a a c c a a t t a a c c a a t t t c g g c g g c a g g g c a g t t c a g t c g c a t t a c g a g a c g g t c a c a a t g t t g g t t a a t t g g t t a a

7080 270 265 260 255 *FFEDLMLHFQLKNMDPND KanR *FFEDLMLHFQLKNMDPND c t g a t t a g a a a a a c t c a t c g a g c a t c a a a t g a a a c t g c a a t t t a t t c a t a t c a g g a t t a t g a c t a a t c t t t t t g a g t a g c t c g t a g t t t a c t t t g a c g t t a a a t a a g t a t a g t c c t a a t a

7140 250 245 240 235 IGYKQFLRKQLSPSFEGLCN KanR IGYKQFLRKQLSPSFEGLCN c a a t a c c a t a t t t t t g a a a a a g c c g t t t c t g t a a t g a a g g a g a a a a c t c a c c g a g g c a g t g t t a t g g t a t a a a a a c t t t t t c g g c a a a g a c a t t a c t t c c t c t t t t g a g t g g c t c c g t c a

PflMI 7200 230 225 220 215 WLIALDQYRDAIGVRGVDIC KanR WLIALDQYRDAIGVRGVDIC t c c a t a g g a t g g c a a g a t c c t g g t a t c g g t c t g c g a t t c c g a c t c g t c c a a c a t c a a t a c a g g t a t c c t a c c g t t c t a g g a c c a t a g c c a g a c g c t a a g g c t g a g c a g g t t g t a g t t a t g

7260 210 205 200 195 GILKGEDFILNDLSFDGHTV KanR GILKGEDFILNDLSFDGHTV a a c c t a t t a a t t t c c c c t c g t c a a a a a t a a g g t t a t c a a g t g a g a a a t c a c c a t g a g t g a t t g g a t a a t t a a a g g g g a g c a g t t t t t a t t c c a a t a g t t c a c t c t t t a g t g g t a c t c a c t

7320 190 185 180 175 VSDPSFPLLKHMEKWVQEVP KanR VSDPSFPLLKHMEKWVQEVP c g a c t g a a t c c g g t g a g a a t g g c a a a a g c t t a t g c a t t t c t t t c c a g a c t t g t t c a a c a g g c t g a c t t a g g c c a c t c t t a c c g t t t t c g a a t a c g t a a a g a a a g g t c t g a a c a a g t t g t c

7380 170 165 160 155 WGNREDDFDSADVLGNNMRS KanR WGNREDDFDSADVLGNNMRS g c c a g c c a t t a c g c t c g t c a t c a a a a t c a c t c g c a t c a a c c a a a c c g t t a t t c a t t c g t g c g g t c g g t a a t g c g a g c a g t a g t t t t a g t g a g c g t a g t t g g t t t g g c a a t a a g t a a g c a c

AsiSI 7440 150 145 140 135 QAQALRFVRDSNFPCNCVPI KanR QAQALRFVRDSNFPCNCVPI a t t g c g c c t g a g c g a g a c g a a a t a c g c g a t c g c t g t t a a a a g g a c a a t t a c a a a c a g g a a t a a c g c g g a c t c g c t c t g c t t t a t g c g c t a g c g a c a a t t t t c c t g t t a a t g t t t g t c c t t

7500 130 125 120 115 SHLRRLFVALADVINEGSDP KanR SHLRRLFVALADVINEGSDP t c g a a t g c a a c c g g c g c a g g a a c a c t g c c a g c g c a t c a a c a a t a t t t t c a c c t g a a t c a g a g c t t a c g t t g g c c g c g t c c t t g t g a c g g t c g c g t a g t t g t t a t a a a a g t g g a c t t a g t c

7560 110 105 100 95 YEELVQFATKGPIATTLLWA KanR YEELVQFATKGPIATTLLWA g a t a t t c t t c t a a t a c c t g g a a t g c t g t t t t c c c g g g g a t c g c a g t g g t g a g t a a c c a t g c t a t a a g a a g a t t a t g g a c c t t a c g a c a a a a g g g c c c c t a g c g t c a c c a c t c a t t g g t a c

7620 90 85 80 75 DDPTRIFHKITPLPMFETLW KanR DDPTRIFHKITPLPMFETLW c a t c a t c a g g a g t a c g g a t a a a a t g c t t g a t g g t c g g a a g a g g c a t a a a t t c c g t c a g c c g t a g t a g t c c t c a t g c c t a t t t t a c g a a c t a c c a g c c t t c t c c g t a t t t a a g g c a g t c g g

7680 70 65 60 55 NLRVMEDTVDNAVSGKGHKL KanR NLRVMEDTVDNAVSGKGHKL a g t t t a g t c t g a c c a t c t c a t c t g t a a c a t c a t t g g c a a c g c t a c c t t t g c c a t g t t t c a t c a a a t c a g a c t g g t a g a g t a g a c a t t g t a g t a a c c g t t g c g a t g g a a a c g g t a c a a a g t

7740 50 45 40 35 FLEPADPKGYLRYITAGSQG KanR FLEPADPKGYLRYITAGSQG g a a a c a a c t c t g g c g c a t c g g g c t t c c c a t a c a a t c g a t a g a t t g t c g c a c c t g a t t g c c c t t t g t t g a g a c c g c g t a g c c c g a a g g g t a t g t t a g c t a t c t a a c a g c g t g g a c t a a c g g

7800 30 25 20 15 VNDRAWKYGYLDADMNSNLR KanR A T C G C G A G C C C A T T T A T A C C Kan-R VNDRAWKYGYLDADMNSNLR c g a c a t t a t c g c g a g c c c a t t t a t a c c c a t a t a a a t c a g c a t c c a t g t t g g a a t t t a a t c g c t g t a a t a g c g c t c g g g t a a a t a t g g g t a t a t t t a g t c g t a g g t a c a a c c t t a a a t t a g

7860 10 5 1 PKSCSTERQIHSM KanR A T G G C T C A T A A C A C C C C T T G pENTR-R PKSCSTERQIHSM g c g g c t t c g a g c a a g a c g t t t c c c g t t g a a t a t g g c t c a t a a c a c c c c t t g t a t t a c t g t c g c c g a a g c t c g t t c t g c a a a g g g c a a c t t a t a c c g a g t a t t g t g g g g a a c a t a a t g a c a

7920 t t a t g t a a g c a g a c a g t t t t a t t g t t c a t g a t g a t a t a t t t t t a t c t t g t g c a a t g t a a c a a t a c a t t c g t c t g t c a a a a t a a c a a g t a c t a c t a t a t a a a a a t a g a a c a c g t t a c a t t g

DraIII 7980 a t c a g a g a t t t t g a g a c a c a a c g t g g c t t t g t t g a a t a a a t c g a a c t t t t g c t g a g t t g a t a g t c t c t a a a a c t c t g t g t t g c a c c g a a a c a a c t t a t t t a g c t t g a a a a c g a c t c a a c t

8040 a g g a t c a g a t c a c g c a t c t t c c c g a c a a c g c a g a c c g t t c c g t g g c a a a g c a a a a g t t c a t c c t a g t c t a g t g c g t a g a a g g g c t g t t g c g t c t g g c a a g g c a c c g t t t c g t t t t c a a g t

8100 a a a t c a c c a a c t g g t c c a c c t a c a a c a a a g c t c t c a t c a a c c g t g g c t c c c t c a c t t t c t t t t a g t g g t t g a c c a g g t g g a t g t t g t t t c g a g a g t a g t t g g c a c c g a g g g a g t g a a a g a

8160 g g c t g g a t g a t g g g g c g a t t c a g g c c t g g t a t g a g t c a g c a a c a c c t t c t t c a c g a g g c a c c g a c c t a c t a c c c c g c t a a g t c c g g a c c a t a c t c a g t c g t t g t g g a a g a a g t g c t c c g t

3ʹ 5ʹ 8166 g a c c t c c t g g a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.