Addgene mEos3.2-CCPB1-C-18 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: mEos3.2-CCPB1-C-18 Sequencing Result


Map View

800 600 400 200 End (871) PflFI - Tth111I (853) StyI (788) PvuII - AlwNI - MspA1I (777) Esp3I - BsmBI (752) TaqII (740) AvaI - SmlI - XhoI - BsoBI - PaeR7I (725) BglII (721) BspEI * (712) NspI - SphI (673) PluTI (599) SfoI (597) NarI * (596) KasI (595) Bpu10I (569) AcuI - Eco57MI (534) BanII (455) KpnI (401) Acc65I (397) MslI (355) BstBI (317) HindIII (306) Bsu36I (284) BsmI (211) EcoRV (198) EciI (188) BaeGI - Bme1580I (95) AgeI - BsrFI (21) AfeI (17) BmtI (16) NheI (12) Start (0) mEos3.2 mEos3.2-CCPB1-C-18 871 bp

Sequence View

BsrFIAgeINheIBmtIAfeIStart (0) 60 1 5 MSAIKPDMK mEos3.2 PSDPLALPVATMSAIKPDMK *RDGGHTRNLWVHL c c g t c a g a t c c g c t a g c g c t a c c g g t c g c c a c c a t g a g t g c g a t t a a g c c a g a c a t g a a g g g c a g t c t a g g c g a t c g c g a t g g c c a g c g g t g g t a c t c a c g c t a a t t c g g t c t g t a c t t c

Bme1580IBaeGI 120 10 15 20 25 IKLRMEGNVNGHHFVIDGDG mEos3.2 IKLRMEGNVNGHHFVIDGDG DFETHFAVYVPVVKHDVSIT a t c a a a c t c c g t a t g g a a g g c a a c g t a a a c g g g c a c c a c t t t g t g a t c g a c g g a g a t g g t t a g t t t g a g g c a t a c c t t c c g t t g c a t t t g c c c g t g g t g a a a c a c t a g c t g c c t c t a c c a

180 30 35 40 45 TGKPFEGKQSMDLEVKEGGP mEos3.2 TGKPFEGKQSMDLEVKEGGP CALRKLSFLTHIKFDFLASR a c a g g c a a g c c t t t t g a g g g a a a a c a g a g t a t g g a t c t t g a a g t c a a a g a g g g c g g a c c t t g t c c g t t c g g a a a a c t c c c t t t t g t c t c a t a c c t a g a a c t t c a g t t t c t c c c g c c t g g a

BsmIEcoRVEciI 240 50 55 60 65 LPFAFDILTTAFHYGNRVFA mEos3.2 LPFAFDILTTAFHYGNRVFA QRKGKIDQGSCEMVAVPYEG c t g c c t t t t g c c t t t g a t a t c c t g a c c a c t g c a t t c c a t t a c g g c a a c a g g g t a t t c g c c g a c g g a a a a c g g a a a c t a t a g g a c t g g t g a c g t a a g g t a a t g c c g t t g t c c c a t a a g c g g

Bsu36I 300 70 75 80 85 KYPDNIQDYFKQSFPKGYSW mEos3.2 KYPDNIQDYFKQSFPKGYSW FIWVVYLVIKLLRKRLPIRP a a a t a t c c a g a c a a c a t a c a a g a c t a t t t t a a g c a g t c g t t t c c t a a g g g g t a t t c g t g g t t t a t a g g t c t g t t g t a t g t t c t g a t a a a a t t c g t c a g c a a a g g a t t c c c c a t a a g c a c c

MslIBstBIHindIII 360 90 95 100 105 ERSLTFEDGGICNARNDITM mEos3.2 ERSLTFEDGGICNARNDITM FSAQSEFVPANAVGSVVYCH g a a c g a a g c t t g a c t t t c g a a g a c g g g g g c a t t t g c a a c g c c a g a a a c g a c a t a a c a a t g c t t g c t t c g a a c t g a a a g c t t c t g c c c c c g t a a a c g t t g c g g t c t t t g c t g t a t t g t t a c

Acc65IKpnI 420 110 115 120 125 EGDTFYNKVRFYGTNFPANG mEos3.2 EGDTFYNKVRFYGTNFPANG FPVSEIIFNSKITGVKGGIT g a a g g g g a c a c t t t c t a t a a t a a a g t t c g a t t t t a t g g t a c c a a c t t t c c c g c c a a t g g t c t t c c c c t g t g a a a g a t a t t a t t t c a a g c t a a a a t a c c a t g g t t g a a a g g g c g g t t a c c a

BanII 480 130 135 140 145 PVMQKKTLKWEPSTEKMYVR mEos3.2 PVMQKKTLKWEPSTEKMYVR WNHLLLRQFPLGGSLFHIHT c c a g t t a t g c a g a a g a a g a c g c t g a a a t g g g a g c c c t c c a c t g a g a a a a t g t a t g t g c g t g g t c a a t a c g t c t t c t t c t g c g a c t t t a c c c t c g g g a g g t g a c t c t t t t a c a t a c a c g c a

Eco57MIAcuI 540 150 155 160 165 DGVLTGDIEMALLLEGNAHY mEos3.2 DGVLTGDIEMALLLEGNAHY ISHQRTINLHSQQKFSIGM g a t g g a g t g c t g a c g g g t g a t a t t g a g a t g g c t t t g t t g c t t g a a g g a a a t g c c c a t t a c c t a c c t c a c g a c t g c c c a c t a t a a c t c t a c c g a a a c a a c g a a c t t c c t t t a c g g g t a a t g

KasINarI*SfoIPluTIBpu10I 600 170 175 180 185 RCDFRTTYKAKEKGVKLPGA mEos3.2 RCDFRTTYKAKEKGVKLPGA c g a t g t g a c t t c a g a a c t a c t t a c a a a g c t a a g g a g a a g g g t g t c a a g t t a c c a g g c g c c g c t a c a c t g a a g t c t t g a t g a a t g t t t c g a t t c c t c t t c c c a c a g t t c a a t g g t c c g c g g

660 190 195 200 205 HFVDHCIEILSHDKDYNKVK mEos3.2 HFVDHCIEILSHDKDYNKVK c a c t t t g t g g a c c a c t g c a t t g a g a t t t t a a g c c a t g a c a a a g a t t a c a a c a a g g t t a a g g t g a a a c a c c t g g t g a c g t a a c t c t a a a a t t c g g t a c t g t t t c t a a t g t t g t t c c a a t t c

BspEI*SphINspI 720 210 215 220 225 LYEHAVAHSGLPDNARR SGL mEos3.2 LYEHAVAHSGLPDNARRSGL c t g t a t g a g c a t g c t g t t g c t c a t t c t g g a t t g c c t g a c a a t g c c a g a c g a t c c g g a c t c g a c a t a c t c g t a c g a c a a c g a g t a a g a c c t a a c g g a c t g t t a c g g t c t g c t a g g c c t g a g

MspA1IAlwNIPvuIIBsmBIEsp3ITaqIIBglIIPaeR7IBsoBIXhoISmlIAvaI 780 (in frame with mEos3.2) RSRESSGYSSRSGGSSDQQL RSRESSGYSSRSGGSSDQQL a g a t c t c g a g a g t c c a g t g g a t a t t c g t c t c g g t c g g g t g g a a g c a g t g a c c a g c a g c t g t c t a g a g c t c t c a g g t c a c c t a t a a g c a g a g c c a g c c c a c c t t c g t c a c t g g t c g t c g a c

StyI 840 (in frame with mEos3.2) DCALDLMRRLPPQQIEKNLS DCALDLMRRLPPQQIEKNLS g a t t g t g c c t t g g a t t t g a t g a g g c g t c t g c c t c c g c a g c a g a t a g a g a a g a a t c t c a g t c t a a c a c g g a a c c t a a a c t a c t c c g c a g a c g g a g g c g t c g t c t a t c t c t t c t t a g a g t c a

End (871) Tth111IPflFI 871 (in frame with mEos3.2) DLIDLVPSLC DLIDLVPSLC g a c c t c a t t g a c t t g g t c c c a a g c c t c t g t g c t g g a g t a a c t g a a c c a g g g t t c g g a g a c a c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.