Addgene Str-KDEL_SBP-EGFP-GPI - Sequence Analyzer Skip to main content
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Sequence Analyzer: Str-KDEL_SBP-EGFP-GPI


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SgrDI (6045) SspI (5928) Amp-R (5674 .. 5693) PvuI (5494) FspI (5346) AhdI (5124) pBR322ori-F (4372 .. 4391) DrdI (4339) L4440 (4121 .. 4138) BspQI - SapI (4115) CAP binding site M13/pUC Reverse (3903 .. 3925) lac operator M13 Reverse (3890 .. 3906) BstZ17I (3852) SV40pA-R (3704 .. 3723) PsiI (3686) BclI * (3560) XbaI (3550) PacI (3545) SfiI (3533) AbsI - PaeR7I - PspXI - XhoI (3529) FseI (3432) NaeI (3430) NgoMIV (3428) EGFP-C (3363 .. 3384) EXFP-R (3016 .. 3035) pRS-marker (44 .. 63) NruI (208) SpeI (249) CMV enhancer NdeI (484) SnaBI (590) NcoI (610) CMV-F (769 .. 789) T7 (863 .. 882) EcoRV (914) NheI (927) BmtI (931) AfeI (932) AflII (934) AgeI (948) EcoNI (1005) KasI (1062) NarI (1063) SfoI (1064) PluTI (1066) PmeI (1072) PasI (1292) RsrII (1557) EagI - NotI (1579) SacII (1736) MscI (1827) pMT2-F (1858 .. 1877) SphI (1911) NsiI (1913) PspOMI (2046) ApaI (2050) AvrII (2084) IRES reverse (2100 .. 2117) PmlI (2249) DraIII (2296) IRES-F (2327 .. 2346) BmgBI (2476) TspMI - XmaI (2518) SmaI (2520) AscI - BssHII (2526) EcoRI (2586) SbfI (2709) EGFP-N (2755 .. 2776) PfoI * (2990) Str-KDEL_SBP-EGFP-GPI 6047 bp

Sequence View

5ʹ 3ʹ 60 A T G T T A G A C G A G A C T A C pRS-marker g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t c g a c t c t c a g t a c a a t c t g c t c t g a t g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a g c t g a g a g t c a t g t t a g a c g a g a c t a c

120 G G C pRS-marker c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c

180 c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g

NruI 240 CMV enhancer t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a

SpeI 300 CMV enhancer g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t

360 CMV enhancer t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g

420 CMV enhancer c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g

480 CMV enhancer a t t g a c g t c a a t g g g t g g a c t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t t a a c t g c a g t t a c c c a c c t g a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a

NdeI 540 CMV enhancer a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a

SnaBI 600 CMV enhancer a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t

NcoI 660 CMV enhancer CMV promoter t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c

720 CMV promoter a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g

780 CMV promoter C G C A A A T G G G C G CMV-F a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c

840 CMV promoter G T A G G C G T G CMV-F g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c t c t g g c t a a c t a g a g a a c c c a c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g a g a c c g a t t g a t c t c t t g g g t

900 T7 promoter T A A T A C G A C T C A C T A T A G G G T7 c t g c t t a c t g g c t t a t c g a a a t t a a t a c g a c t c a c t a t a g g g a g a c c c a a g c t t g g t a c c g a c g a a t g a c c g a a t a g c t t t a a t t a t g c t g a g t g a t a t c c c t c t g g g t t c g a a c c a t g g

AgeINheIBmtIAfeIAflIIEcoRV 960 MD g a g c t c g g a t c g a t a t c t g c g g c c t a g c t a g c g c t t a a g g c c t g t t a a c c g g t a t g g a t g c t c g a g c c t a g c t a t a g a c g c c g g a t c g a t c g c g a a t t c c g g a c a a t t g g c c a t a c c t a c

EcoNI 1020 VCVRLALWLLWGLLLHQGQS t a t g c g t c c g t c t t g c c c t g t g g c t c c t c t g g g g a c t c c t c c t g c a c c a g g g c c a g a g c c a t a c g c a g g c a g a a c g g g a c a c c g a g g a g a c c c c t g a g g a g g a c g t g g t c c c g g t c t c g g

PmeIKasINarISfoIPluTI 1080 LSHSHSEKATGTSSGAGLND t c a g c c a t a g t c a c a g t g a g a a g g c g a c a g g a a c c a g c t c g g g c g c c g g t t t a a a c g a c c a g t c g g t a t c a g t g t c a c t c t t c c g c t g t c c t t g g t c g a g c c c g c g g c c a a a t t t g c t g g

1140 PSKDSKAQVSAAEAGITGTW c t a g c a a a g a c t c a a a a g c t c a g g t g t c c g c t g c c g a g g c t g g c a t t a c t g g a a c a t g g t g a t c g t t t c t g a g t t t t c g a g t c c a c a g g c g a c g g c t c c g a c c g t a a t g a c c t t g t a c c a

1200 YNQLGSTFIVTAGADGALTG a c a a t c a g c t c g g g a g c a c c t t t a t t g t g a c t g c t g g a g c c g a t g g a g c c c t c a c c g g a a t g t t a g t c g a g c c c t c g t g g a a a t a a c a c t g a c g a c c t c g g c t a c c t c g g g a g t g g c c t t

1260 TYESAVGNAESRYVLTGRYD c a t a c g a a t c t g c t g t g g g a a a c g c c g a a t c a c g g t a c g t c c t c a c t g g c c g a t a c g a t a g t a t g c t t a g a c g a c a c c c t t t g c g g c t t a g t g c c a t g c a g g a g t g a c c g g c t a t g c t a t

PasI 1320 SAPATDGSGTALGWTVAWKN g t g c c c c t g c c a c c g a c g g a t c t g g g a c t g c c c t g g g a t g g a c t g t c g c t t g g a a a a a c a c a c g g g g a c g g t g g c t g c c t a g a c c c t g a c g g g a c c c t a c c t g a c a g c g a a c c t t t t t g t

1380 NYRNAHSATTWSGQYVGGAE a c t a c c g g a a t g c t c a t t c t g c c a c a a c a t g g a g t g g a c a g t a c g t g g g a g g c g c t g a g g t g a t g g c c t t a c g a g t a a g a c g g t g t t g t a c c t c a c c t g t c a t g c a c c c t c c g c g a c t c c

1440 ARINTQWLLTSGTTEANAWK c t a g a a t c a a t a c a c a g t g g c t g c t c a c a t c t g g c a c a a c c g a g g c a a a t g c t t g g a a a t g a t c t t a g t t a t g t g t c a c c g a c g a g t g t a g a c c g t g t t g g c t c c g t t t a c g a a c c t t t a

1500 STLVGHDTFTKVKPSAASID c c a c c c t g g t g g g a c a t g a c a c a t t c a c c a a a g t g a a a c c c t c c g c c g c t t c a a t c g a t g g g t g g g a c c a c c c t g t a c t g t g t a a g t g g t t t c a c t t t g g g a g g c g g c g a a g t t a g c t a c

RsrII 1560 AAKKAGVNNGNPLDAVQQLG c c g c c a a a a a a g c c g g a g t c a a c a a c g g c a a t c c t c t g g a t g c c g t c c a g c a g c t c g g a c g g c g g t t t t t t c g g c c t c a g t t g t t g c c g t t a g g a g a c c t a c g g c a g g t c g t c g a g c c t g

EagINotI 1620 PKDEL* c g a a a g a t g a a c t g t g a g c g g c c g c a t a g a t a a c t g a t c c a g t g t g c t g g a a t t a a t t c g g c t t t c t a c t t g a c a c t c g c c g g c g t a t c t a t t g a c t a g g t c a c a c g a c c t t a a t t a a g c

1680 chimeric intron c t g t c t g c g a g g g c c a g c t g t t g g g g t g a g t a c t c c c t c t c a a a a g c g g g c a t g a c t t c t g a c a g a c g c t c c c g g t c g a c a a c c c c a c t c a t g a g g g a g a g t t t t c g c c c g t a c t g a a g a

SacII 1740 chimeric intron g c g c t a a g a t t g t c a g t t t c c a a a a a c g a g g a g g a t t t g a t a t t c a c c t g g c c c g c g g t g c g c g a t t c t a a c a g t c a a a g g t t t t t g c t c c t c c t a a a c t a t a a g t g g a c c g g g c g c c a c

1800 chimeric intron a t g c c t t t g a g g g t g g c c g c g t c c a t c t g g t c a g a a a a g a c a a t c t t t t t g t t g t c a a g c t a c g g a a a c t c c c a c c g g c g c a g g t a g a c c a g t c t t t t c t g t t a g a a a a a c a a c a g t t c g

MscI 1860 chimeric intron T T G pMT2-F t t g a g g t g t g g c a g g c t t g a g a t c t g g c c a t a c a c t t g a g t g a c a a t g a c a t c c a c t t t g a a c t c c a c a c c g t c c g a a c t c t a g a c c g g t a t g t g a a c t c a c t g t t a c t g t a g g t g a a a c

SphINsiI 1920 chimeric intron C C T T T C T C T C C A C A G G T pMT2-F c c t t t c t c t c c a c a g g t g t c c a c t c c c a g g t c c a a c t g c a g g t c g a g c a t g c a t c t a g g g g g a a a g a g a g g t g t c c a c a g g t g a g g g t c c a g g t t g a c g t c c a g c t c g t a c g t a g a t c c c

1980 IRES2 c g g c c a a t t c c g c c c c t c t c c c t c c c c c c c c c c t a a c g t t a c t g g c c g a a g c c g c t t g g a g c c g g t t a a g g c g g g g a g a g g g a g g g g g g g g g g a t t g c a a t g a c c g g c t t c g g c g a a c c t

2040 IRES2 a t a a g g c c g g t g t g c g t t t g t c t a t a t g t t a t t t t c c a c c a t a t t g c c g t c t t t t g g c a a t a t t c c g g c c a c a c g c a a a c a g a t a t a c a a t a a a a g g t g g t a t a a c g g c a g a a a a c c g t t

AvrIIPspOMIApaI 2100 IRES2 G IRES reverse t g t g a g g g c c c g g a a a c c t g g c c c t g t c t t c t t g a c g a g c a t t c c t a g g g g t c t t t c c c c a c a c t c c c g g g c c t t t g g a c c g g g a c a g a a g a a c t g c t c g t a a g g a t c c c c a g a a a g g g g

2160 IRES2 A G A G C G G T T T C C T T A C G IRES reverse t c t c g c c a a a g g a a t g c a a g g t c t g t t g a a t g t c g t g a a g g a a g c a g t t c c t c t g g a a g c a g a g c g g t t t c c t t a c g t t c c a g a c a a c t t a c a g c a c t t c c t t c g t c a a g g a g a c c t t c g

2220 IRES2 t t c t t g a a g a c a a a c a a c g t c t g t a g c g a c c c t t t g c a g g c a g c g g a a c c c c c c a c c t g g a a g a a c t t c t g t t t g t t g c a g a c a t c g c t g g g a a a c g t c c g t c g c c t t g g g g g g t g g a c c

PmlI 2280 IRES2 c g a c a g g t g c c t c t g c g g c c a a a a g c c a c g t g t a t a a g a t a c a c c t g c a a a g g c g g c a c a g c t g t c c a c g g a g a c g c c g g t t t t c g g t g c a c a t a t t c t a t g t g g a c g t t t c c g c c g t g t

DraIII 2340 IRES2 T G G C T C T C C T C A A G IRES-F a c c c c a g t g c c a c g t t g t g a g t t g g a t a g t t g t g g a a a g a g t c a a a t g g c t c t c c t c a a g t g g g g t c a c g g t g c a a c a c t c a a c c t a t c a a c a c c t t t c t c a g t t t a c c g a g a g g a g t t c

2400 IRES2 C G T A T T IRES-F c g t a t t c a a c a a g g g g c t g a a g g a t g c c c a g a a g g t a c c c c a t t g t a t g g g a t c t g a t c t g c a t a a g t t g t t c c c c g a c t t c c t a c g g g t c t t c c a t g g g g t a a c a t a c c c t a g a c t a g a

2460 IRES2 MLYMCLVEVKKTSRP g g g g c c t c g g t g c a c a t g c t t t a c a t g t g t t t a g t c g a g g t t a a a a a a a c g t c t a g g c c c c c c c g g a g c c a c g t g t a c g a a a t g t a c a c a a a t c a g c t c c a a t t t t t t t g c a g a t c c g g g

TspMIXmaISmaIBmgBI 2520 ATG IRES2 PEPRGRGFPLKNTMISLPQP c c c g a a c c a c g g g g a c g t g g t t t t c c t t t g a a a a a c a c g a t g a t a a g c t t g c c a c a a c c c g g g c t t g g t g c c c c t g c a c c a a a a g g a a a c t t t t t g t g c t a c t a t t c g a a c g g t g t t g g g

BssHIIAscI 2580 1 5 10 15 MYRMQLLSCIALSLAL IL-2 signal sequence GRRAMYRMQLLSCIALSLAL g g g a g g c g c g c c a t g t a c a g g a t g c a a c t c c t g t c t t g c a t t g c a c t a a g t c t t g c a c t t c c c t c c g c g c g g t a c a t g t c c t a c g t t g a g g a c a g a a c g t a a c g t g a t t c a g a a c g t g a a

EcoRI 2640 20 VTNS 5 10 15 DEKTTGWRGGHVVEGL IL-2 signal sequence SBP VTNSDEKTTGWRGGHVVEGL g t c a c g a a t t c c g a c g a g a a g a c c a c t g g t t g g c g a g g t g g a c a c g t t g t t g a a g g a c t g c a g t g c t t a a g g c t g c t c t t c t g g t g a c c a a c c g c t c c a c c t g t g c a a c a a c t t c c t g a c

2700 20 25 30 35 AGELEQLRARLEHHPQGQRE SBP AGELEQLRARLEHHPQGQRE g c t g g g g a a c t t g a a c a a c t t c g t g c a c g a c t g g a g c a t c a c c c a c a a g g t c a a c g t g a a c g a c c c c t t g a a c t t g t t g a a g c a c g t g c t g a c c t c g t a g t g g g t g t t c c a g t t g c a c t t

SbfI 2760 P PAG 1a V 5 10 15 SKGEELFTGVVPILV SBP EGFP 1a V 5 10 15 SKGEELFTGVVPILV EGFP G A C C A G EGFP-N PPAGVSKGEELFTGVVPILV c c a c c t g c a g g t g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g g t g g a c g t c c a c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g

2820 20 25 30 35 ELDGDVNGHKFSVSGEGEGD EGFP 20 25 30 35 ELDGDVNGHKFSVSGEGEGD EGFP C T C G A C C T G C C G C T G C EGFP-N ELDGDVNGHKFSVSGEGEGD g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a

2880 40 45 50 55 ATYGKLTLKFICTTGKLPVP EGFP 40 45 50 55 ATYGKLTLKFICTTGKLPVP EGFP ATYGKLTLKFICTTGKLPVP g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g

2940 60 65 70 75 WPTLVTTLTYGVQCFSRYPD EGFP 60 65 70 75 WPTLVTTLTYGVQCFSRYPD EGFP WPTLVTTLTYGVQCFSRYPD t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g

PfoI* 3000 80 85 90 95 HMKQHDFFKSAMPEGYVQER EGFP 80 85 90 95 HMKQHDFFKSAMPEGYVQER EGFP HMKQHDFFKSAMPEGYVQER c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g

3060 100 105 110 115 TIFFKDDGNYKTRAEVKFEG EGFP 100 105 110 115 TIFFKDDGNYKTRAEVKFEG EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R TIFFKDDGNYKTRAEVKFEG a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g

3120 120 125 130 135 DTLVNRIELKGIDFKEDGNI EGFP 120 125 130 135 DTLVNRIELKGIDFKEDGNI EGFP DTLVNRIELKGIDFKEDGNI g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g

3180 140 145 150 155 LGHKLEYNYNSHNVYIMADK EGFP 140 145 150 155 LGHKLEYNYNSHNVYIMADK EGFP LGHKLEYNYNSHNVYIMADK c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c

3240 160 165 170 175 QKNGIKVNFKIRHNIEDGSV EGFP 160 165 170 175 QKNGIKVNFKIRHNIEDGSV EGFP QKNGIKVNFKIRHNIEDGSV c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c

3300 180 185 190 195 QLADHYQQNTPIGDGPVLLP EGFP 180 185 190 195 QLADHYQQNTPIGDGPVLLP EGFP QLADHYQQNTPIGDGPVLLP c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g

3360 200 205 210 215 DNHYLSTQSALSKDPNEKRD EGFP 200 205 210 215 DNHYLSTQSALSKDPNEKRD EGFP DNHYLSTQSALSKDPNEKRD g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a

3420 220 225 230 235 HMVLLEFVTAAGITLGMDEL EGFP 220 225 230 235 HMVLLEFVTAAGITLGMDEL EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C HMVLLEFVTAAGITLGMDEL c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c

NgoMIVNaeIFseI 3480 YK (in frame with EGFP) GRPLENGGTSLSEKTVLL EGFP YK (in frame with EGFP) GRPLENGGTSLSEKTVLL EGFP YKGRPLENGGTSLSEKTVLL t a c a a g g g c c g g c c a c t g g a a a a t g g c g g a a c c t c t c t g t c t g a a a a a a c t g t g c t g c t g a t g t t c c c g g c c g g t g a c c t t t t a c c g c c t t g g a g a g a c a g a c t t t t t t g a c a c g a c g a c

PaeR7IPspXIXhoIAbsISfiI 3540 (in frame with EGFP) LVTPFLAAAWSLHP* (in frame with EGFP) LVTPFLAAAWSLHP* LVTPFLAAAWSLHP* c t g g t g a c t c c c t t t c t g g c c g c t g c t t g g t c c c t c c a c c c a t g a g g c c t c g a g g g c c a a g a c c a c t g a g g g a a a g a c c g g c g a c g a a c c a g g g a g g t g g g t a c t c c g g a g c t c c c g g t t

BclI*XbaIPacI 3600 t t a a t t a a c t c t a g a t a a c t g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t a a t t a a t t g a g a t c t a t t g a c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a

3660 g c t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t c g a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a

PsiI 3720 SV40 poly(A) signal C G T T A T C G T A G T G T T T A SV40pA-R g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a

3780 SV40 poly(A) signal A A G SV40pA-R t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a

3840 SV40 poly(A) signal g t a t c t t a a c g c g t c g a g t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t c a t a g a a t t g c g c a g c t c a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a

BstZ17I 3900 M13 rev C A G T A T C G A C A M13 Reverse a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t a a t c a t g g t c a t a g c t g t t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c a t t a g t a c c a g t a t c g a c a

3960 -10 M13 rev lac operator lac promoter A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t

4020 -35 lac promoter CAP binding site a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g

4080 t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c

BspQISapI 4140 G A G C G A G T G A C T G A G C G A L4440 c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g

4200 g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a

4260 c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t

4320 ori g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g

DrdI 4380 ori T T T C T A T G G pBR322ori-F a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g

4440 ori T C C G C A A A G G G pBR322ori-F a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c

4500 ori g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a a t g c t c a c g c t g t a c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t t a c g a g t g c g a c a t

4560 ori g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c

4620 ori t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g

4680 ori a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c

4740 ori g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g g a c a g t a t c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c c t g t c a t a

4800 ori t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a

4860 ori c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g

4920 ori g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a

4980 g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a

5040 a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a

5100 285 280 275 *WHKILSAGIEAIQRNR AmpR *WHKILSAGIEAIQRNR g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g

AhdI 5160 270 265 260 255 EDMTAQSGTTYIVVIRSPKG AmpR EDMTAQSGTTYIVVIRSPKG g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g

5220 250 245 240 235 DPGLAAIIGRSGREGAGSKD AmpR DPGLAAIIGRSGREGAGSKD c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a

5280 230 225 220 215 AIFWGAPLASRLLPGAVKDA AmpR AIFWGAPLASRLLPGAVKDA c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c

5340 210 205 200 195 EMWDILQQRSALTLLEGTLL AmpR EMWDILQQRSALTLLEGTLL c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t

FspI 5400 190 185 180 175 KRLTTAMAVPMTTDREDNPI AmpR KRLTTAMAVPMTTDREDNPI g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t

5460 170 165 160 155 AENLEPEWRDLRTVHDGMNH AmpR AENLEPEWRDLRTVHDGMNH t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a

PvuI 5520 150 145 140 135 LFATLEKPGGITTLLLNAAT AmpR LFATLEKPGGITTLLLNAAT g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c

5580 130 125 120 115 NDSMTIAASCLERVTMGDTL AmpR NDSMTIAASCLERVTMGDTL t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t

5640 110 105 100 95 HKETVPSYEVLDNQSYHIRR AmpR HKETVPSYEVLDNQSYHIRR g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g

5700 90 85 80 75 GLQEQGADIRSLVAGCLLVK AmpR A T A A T A C C G C G C C A C A T A G C Amp-R GLQEQGADIRSLVAGCLLVK g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a

5760 70 65 60 55 FTSMMPFREEPRFSELIKGS AmpR FTSMMPFREEPRFSELIKGS t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g

5820 50 45 40 35 NLDLEIYGVRAGLQDEADKV AmpR NLDLEIYGVRAGLQDEADKV t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t

5880 30 25 KVLTEPH 20 15 signal sequence AFVPLCFAAFFPI AmpR KVLTEPHAFVPLCFAAFFPI c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t

SspI 5940 10 5 1 signal sequence LAVRFHQISM AmpR AmpR promoter LAVRFHQISM t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t

6000 AmpR promoter t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g

SgrDI 3ʹ 5ʹ 6047 AmpR promoter a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.