Addgene pMuLE ENTR CMV eGFP R4-R3 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pMuLE ENTR CMV eGFP R4-R3 Sequencing Result

Map View

800 600 400 200 End (863) NsiI (857) NspI - SphI (855) SmlI - AvaI - XhoI - PspXI - BsoBI - PaeR7I (846) BsiEI (832) NotI - EagI (829) BsrGI (816) EGFP-C (760 .. 781) BmrI (712) EXFP-R (413 .. 432) PfoI * (387) BssSI - BssSαI (287) BsrFI (257) BtgZI (228) EGFP-N (152 .. 173) BseRI (137) StyI - BtgI - NcoI (105) BamHI (91) KpnI (83) Acc65I (79) HindIII (73) BsaI (60) SfcI (59) T7 (47 .. 66) AseI (46) Start (0) T7 promoter EGFP EGFP Kozak sequence pMuLE ENTR CMV eGFP R4-R3 863 bp

Sequence View

SfcIAseIStart (0) 5ʹ 3ʹ 60 T7 promoter T A A T A C G A C T C A C T T7 * c t c t c t g g c t a a c t a g a g a a c c c a c t g c t t a c t g g c t t a t c g a a a t t a a t a c g a c t c a c t g a g a g a c c g a t t g a t c t c t t g g g t g a c g a a t g a c c g a a t a g c t t t a a t t a t g c t g a g t g a

NcoIStyIBtgIBamHIHindIIIAcc65IKpnIBsaI 120 1 M 1a V SKG T7 promoter EGFP 1 M 1a V SKG EGFP Kozak sequence A T A G G G T7 MVSKG LSVWAQYRARIRFGGHHALA a t a g g g a g a c c c a a g c t t g g t a c c g a g c t c g g a t c c t a a a g c c a c c a t g g t g a g c a a g g g t a t c c c t c t g g g t t c g a a c c a t g g c t c g a g c c t a g g a t t t c g g t g g t a c c a c t c g t t c c c

BseRI 180 5 10 15 20 EELFTGVVPILVELDGDVNG EGFP 5 10 15 20 EELFTGVVPILVELDGDVNG EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N EELFTGVVPILVELDGDVNG LLQEGPHHGDQDLQVAVYVA c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c

BtgZI 240 25 30 35 40 HKFSVSGEGEGDATYGKLTL EGFP 25 30 35 40 HKFSVSGEGEGDATYGKLTL EGFP HKFSVSGEGEGDATYGKLTL VLEAHGALALAIGGVALQGQ c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a

BssSαIBssSIBsrFI 300 45 50 55 60 KFICTTGKLPVPWPTLVTTL EGFP 45 50 55 60 KFICTTGKLPVPWPTLVTTL EGFP KFICTTGKLPVPWPTLVTTL LEDAGGALQGHGPGGEHGGQ g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a

360 65 70 75 80 TYGVQCFSRYPDHMKQHDFF EGFP 65 70 75 80 TYGVQCFSRYPDHMKQHDFF EGFP TYGVQCFSRYPDHMKQHDFF GVAHLAEAAVGVVHLLVVEE g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a

PfoI* 420 85 90 95 100 KSAMPEGYVQERTIFFKDDG EGFP 85 90 95 100 KSAMPEGYVQERTIFFKDDG EGFP C T G C T G C C EXFP-R KSAMPEGYVQERTIFFKDDG LGGHGFAVDLLAGDEELVVA c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c

480 105 110 115 120 NYKTRAEVKFEGDTLVNRIE EGFP 105 110 115 120 NYKTRAEVKFEGDTLVNRIE EGFP G T T G A T G T T C T G EXFP-R NYKTRAEVKFEGDTLVNRIE VVLGAGLHLELAVGQHVADL c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t

540 125 130 135 140 LKGIDFKEDGNILGHKLEYN EGFP 125 130 135 140 LKGIDFKEDGNILGHKLEYN EGFP LKGIDFKEDGNILGHKLEYN QLADVELLVAVDQPVLQLVV g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t

600 145 150 155 160 YNSHNVYIMADKQKNGIKVN EGFP 145 150 155 160 YNSHNVYIMADKQKNGIKVN EGFP YNSHNVYIMADKQKNGIKVN VVAVVDIDHGVLLLVADLHV c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t

660 165 170 175 180 FKIRHNIEDGSVQLADHYQQ EGFP 165 170 175 180 FKIRHNIEDGSVQLADHYQQ EGFP FKIRHNIEDGSVQLADHYQQ ELDAVVDLVAAHLEGVVVLL c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t

BmrI 720 185 190 195 200 NTPIGDGPVLLPDNHYLSTQ EGFP 185 190 195 200 NTPIGDGPVLLPDNHYLSTQ EGFP NTPIGDGPVLLPDNHYLSTQ VGGDAVAGHQQGVVVVQAGL g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t

780 205 210 215 220 SALSKDPNEKRDHMVLLEFV EGFP 205 210 215 220 SALSKDPNEKRDHMVLLEFV EGFP C A T G G T C C T G C T G G A G T T C G T EGFP-C SALSKDPNEKRDHMVLLEFV GGQAFVGVLLAIVHDQQLEH g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a

EagINotIBsiEIBsrGI 840 225 230 235 TAAGITLGMDELYK* EGFP 225 230 235 TAAGITLGMDELYK * EGFP G EGFP-C TAAGITLGMDELYK* GGGPDSEAHVLQVLLAAAVR g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g t a a a g c g g c c g c g a c t c c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c a t t t c g c c g g c g c t g a g

End (863) SphINspINsiIPaeR7IBsoBIPspXIXhoISmlIAvaI 3ʹ 5ʹ 863 SSSCADL t a g a g c t c g a g c a t g c a t c t a g a a t c t c g a g c t c g t a c g t a g a t c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.