Addgene mCherry-miniSOG-CDC42-C-10 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: mCherry-miniSOG-CDC42-C-10 Sequencing Result

Map View

800 600 400 200 End (886) BsaBI * (867) BclI * (862) XbaI * (852) BamHI (840) BfuAI - PaqCI - BspMI (811) EarI - SapI - BspQI (807) SspI (781) StuI (754) EcoP15I (657) BsaI (521) BsiEI (468) PstI (440) SfcI (436) ApoI (342) EcoRV (325) NspI (316) PciI - AflIII (312) BtgZI (307) BsmI (268) BglII (243) BspEI (234) TatI BsrGI (226) NmeAIII (214) XcmI (212) BsrFI - SgrAI (207) AleI (168) HincII (125) MmeI (115) PvuII (104) DrdI (66) BbvCI - Bpu10I (64) EaeI (50) Start (0) Cdc42 mCherry-miniSOG-CDC42-C-10 886 bp

Sequence View

EaeIStart (0) 5ʹ 3ʹ 60 GALKGEIKQRLKLKDGGHY a c g g c g c c c t g a a g g g c g a g a t c a a g c a g a g g c t g a a g c t g a a g g a c g g c g g c c a c t a c g t g c c g c g g g a c t t c c c g c t c t a g t t c g t c t c c g a c t t c g a c t t c c t g c c g c c g g t g a t g c

MmeIPvuIIBpu10IBbvCIDrdI 120 DAEVKTTYKAKKPVQLPGAY a c g c t g a g g t c a a g a c c a c c t a c a a g g c c a a g a a g c c c g t g c a g c t g c c c g g c g c c t a c a t g c g a c t c c a g t t c t g g t g g a t g t t c c g g t t c t t c g g g c a c g t c g a c g g g c c g c g g a t g t

AleIHincII 180 NVNIKLDITSHNEDYTIVEQ a c g t c a a c a t c a a g t t g g a c a t c a c c t c c c a c a a c g a g g a c t a c a c c a t c g t g g a a c a g t t g c a g t t g t a g t t c a a c c t g t a g t g g a g g g t g t t g c t c c t g a t g t g g t a g c a c c t t g t c a

BspEIBsrGITatISgrAIBsrFIXcmINmeAIII 240 YERAEGRHSTGGMDELYKSG a c g a a c g c g c c g a g g g c c g c c a c t c c a c c g g c g g c a t g g a c g a g c t g t a c a a g t c c g g a c t g c t t g c g c g g c t c c c g g c g g t g a g g t g g c c g c c g t a c c t g c t c g a c a t g t t c a g g c c t g

BsmIBglII 300 1 5 10 MQTIKCVVVGDG Cdc42 LRSGGSAGMQTIKCVVVGDG t c a g a t c t g g t g g c t c c g c a g g a a t g c a g a c a a t t a a g t g t g t t g t t g t g g g c g a t g g t g a g t c t a g a c c a c c g a g g c g t c c t t a c g t c t g t t a a t t c a c a c a a c a a c a c c c g c t a c c a c

ApoIEcoRVBtgZIAflIIIPciINspI 360 15 20 25 30 AVGKTCLLISYTTNKFPSEY Cdc42 AVGKTCLLISYTTNKFPSEY c t g t t g g t a a a a c a t g t c t c c t g a t a t c c t a c a c a a c a a a c a a a t t t c c a t c g g a a t a t g g a c a a c c a t t t t g t a c a g a g g a c t a t a g g a t g t g t t g t t t g t t t a a a g g t a g c c t t a t a c

420 35 40 45 50 VPTVFDNYAVTVMIGGEPYT Cdc42 VPTVFDNYAVTVMIGGEPYT t a c c g a c t g t t t t t g a c a a c t a t g c a g t c a c a g t t a t g a t t g g t g g a g a a c c a t a t a c t c a t g g c t g a c a a a a a c t g t t g a t a c g t c a g t g t c a a t a c t a a c c a c c t c t t g g t a t a t g a g

BsiEIPstISfcI 480 55 60 65 70 LGLFDTAGQEDYDRLRPLSY Cdc42 LGLFDTAGQEDYDRLRPLSY t t g g a c t t t t t g a t a c t g c a g g g c a a g a g g a t t a t g a c a g a t t a c g a c c g c t g a g t t a t c a a c c t g a a a a a c t a t g a c g t c c c g t t c t c c t a a t a c t g t c t a a t g c t g g c g a c t c a a t a g

BsaI 540 75 80 85 90 PQTDVFLVCFSVVSPSSFEN Cdc42 PQTDVFLVCFSVVSPSSFEN c a c a a a c a g a t g t a t t t c t a g t c t g t t t t t c a g t g g t c t c t c c a t c t t c a t t t g a a a a c g g t g t t t g t c t a c a t a a a g a t c a g a c a a a a a g t c a c c a g a g a g g t a g a a g t a a a c t t t t g c

600 95 100 105 110 VKEKWVPEITHHCPKTPFLL Cdc42 VKEKWVPEITHHCPKTPFLL t g a a a g a a a a g t g g g t g c c t g a g a t a a c t c a c c a c t g t c c a a a g a c t c c t t t c t t g c t t g a c t t t c t t t t c a c c c a c g g a c t c t a t t g a g t g g t g a c a g g t t t c t g a g g a a a g a a c g a a c

660 115 120 125 130 VGTQIDLRDDPSTIEKLAKN Cdc42 VGTQIDLRDDPSTIEKLAKN t t g g g a c t c a a a t t g a t c t c a g a g a t g a c c c c t c t a c t a t t g a g a a a c t t g c c a a g a a c a a a c c c t g a g t t t a a c t a g a g t c t c t a c t g g g g a g a t g a t a a c t c t t t g a a c g g t t c t t g t

720 135 140 145 150 KQKPITPETAEKLARDLKAV Cdc42 KQKPITPETAEKLARDLKAV a a c a g a a g c c t a t c a c t c c a g a g a c t g c t g a a a a g c t g g c c c g t g a c c t g a a g g c t g t c a t t g t c t t c g g a t a g t g a g g t c t c t g a c g a c t t t t c g a c c g g g c a c t g g a c t t c c g a c a g t

StuI 780 155 160 165 170 KYVECSALTQKGLKNVFDEA Cdc42 KYVECSALTQKGLKNVFDEA a g t a t g t g g a g t g t t c t g c a c t t a c a c a g a a a g g c c t a a a g a a t g t a t t t g a c g a a g c a a t c a t a c a c c t c a c a a g a c g t g a a t g t g t c t t t c c g g a t t t c t t a c a t a a a c t g c t t c g t t

BspQISapIEarIBspMIPaqCIBfuAISspI 840 175 180 185 190 ILAALEPPEPKKSRRCVLL* Cdc42 ILAALEPPEPKKSRRCVLL* t a t t g g c t g c c c t g g a g c c t c c a g a a c c g a a g a a g a g c c g c a g g t g t g t g c t g c t a t g a g a t a a c c g a c g g g a c c t c g g a g g t c t t g g c t t c t t c t c g g c g t c c a c a c a c g a c g a t a c t c

End (886) BsaBI*BclI*XbaI*BamHI 3ʹ 5ʹ 886 g a t c c a c c g g a t c t a g a t a a c t g a t c a t a a t c a g c c a t a c c a c a t t c t a g g t g g c c t a g a t c t a t t g a c t a g t a t t a g t c g g t a t g g t g t a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.