Addgene MXS_mKO2 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: MXS_mKO2 Sequencing Result

Map View

600 400 200 End (709) EcoRV (703) MluI (693) SmlI - AvaI - XhoI - BsoBI - PaeR7I (684) DrdI (633) MfeI (605) BtsI - BtsαI (561) NmeAIII (518) AhdI (507) PmlI - BsaAI (497) EarI (459) MmeI (419) StuI (417) BstBI (388) FspI - FspAI (360) ApoI (315) HpaI (305) BmrI (304) BbsI (144) BsaI (113) BtgZI (105) BfuAI - BspMI (61) AccI (22) SalI (21) Start (0) mKO2 MXS_mKO2 709 bp

Sequence View

SalIAccIStart (0) 5ʹ 3ʹ 60 (in frame with mKO2) CMEGFQDVSY 215 210 HAVADEVQE mKO2 CMEGFQDVSYHAVADEVQE g c g c a c a t t t c c c c g a a t t g g t c g a c t g a g t a a t g a g c t a c t g c a t c t t c t a c c t g c t c a c g c g t g t a a a g g g g c t t a a c c a g c t g a c t c a t t a c t c g a t g a c g t a g a a g a t g g a c g a g t

BsaIBtgZIBspMIBfuAI 120 205 200 195 190 TINGETKRVLRHGIYHDGPM mKO2 TINGETKRVLRHGIYHDGPM g t a a t g t t g c c t t c g g t t t t c c t g a c g a g g c g a t g g c c g a t g t a a t g g t c t c c t g g c a t t c a t t a c a a c g g a a g c c a a a a g g a c t g c t c c g c t a c c g g c t a c a t t a c c a g a g g a c c g t a a

BbsI 180 185 180 175 170 ELIEKAAKYTTKMQCKHNGG mKO2 ELIEKAAKYTTKMQCKHNGG t c a a g a a t c t c t t t t g c c g c c t t g t a a g t a g t c t t c a t t t g g c a t t t g t g a t t g c c g c c t a g t t c t t a g a g a a a a c g g c g g a a c a t t c a t c a g a a g t a a a c c g t a a a c a c t a a c g g c g g a

240 165 160 155 150 GELKLYMTVDGKLVGDSATI mKO2 GELKLYMTVDGKLVGDSATI c c t t c a a g t t t t a g g t a c a t c g t a a c a t c a c c c t t c a g a a c t c c g t c g c t g g c a g t a a t t g g a a g t t c a a a a t c c a t g t a g c a t t g t a g t g g g a a g t c t t g a g g c a g c g a c c g t c a t t a a

300 145 140 135 130 KETSPEWDVSQNQMIPGDAP mKO2 KETSPEWDVSQNQMIPGDAP t t c t c g g t t g a t g g c t c c c a a t c a a c a c t t t g g t t t t g c a t g a t a g g a c c a t c g g c a g g a a a g a g c c a a c t a c c g a g g g t t a g t t g t g a a a c c a a a a c g t a c t a t c c t g g t a g c c g t c c t

FspAIFspIApoIBmrIHpaI 360 125 120 115 110 FNVGTFKSKHYFTNGRLSIH mKO2 FNVGTFKSKHYFTNGRLSIH a a g t t a a c c c c a g t a a a t t t g g a t t t g t g g t a g a a g g t g t t t c c t c t a a g g c t t a t a t g c t t c a a t t g g g g t c a t t t a a a c c t a a a c a c c a t c t t c c a c a a a g g a g a t t c c g a a t a t a c g

StuIMmeIBstBI 420 105 100 95 90 ASVSASGGDEFELSREWSLG mKO2 ASVSASGGDEFELSREWSLG g c a c t g a c t g a a g c g g a c c c a c c a t c t t c g a a c t c c a a c g a c c t t t c c c a t g a c a g g c c t c g t g a c t g a c t t c g c c t g g g t g g t a g a a g c t t g a g g t t g c t g g a a a g g g t a c t g t c c g g a

EarI 480 85 80 75 70 EPFAQKFYDPIEEPYKTFVR mKO2 EPFAQKFYDPIEEPYKTFVR t c a g g a a a t g c t t g t t t g a a a t a g t c t g g t a t c t c t t c t g g a t a t t t a g t a a a t a c t c t g a g t c c t t t a c g a a c a a a c t t t a t c a g a c c a t a g a g a a g a c c t a t a a a t c a t t t a t g a g a c

NmeAIIIAhdIBsaAIPmlI 540 65 60 55 50 HGYCFVHSVLDFAFPMPGGE mKO2 HGYCFVHSVLDFAFPMPGGE t g g c c g t a a c a g a a c a c g t g t g a c a c t a a g t c a a a c g c g a a a g g c a t t g g c c c g c c c t c g a c c g g c a t t g t c t t g t g c a c a c t g t g a t t c a g t t t g c g c t t t c c g t a a c c g g g c g g g a g c

BtsαIBtsI 600 45 40 35 30 AMTVRLTMEQHGEYPRGTGE mKO2 AMTVRLTMEQHGEYPRGTGE g c c a t t g t g a c g c g c a g t g t c a t c t c t t g a t g t c c c t c g t a a g g t c t g c c t g t g c c t t c a c g g t a a c a c t g c g c g t c a c a g t a g a g a a c t a c a g g g a g c a t t c c a g a c g g a c a c g g a a g t

DrdIMfeI 660 25 20 15 10 GEITFEHGNVSGDMYYRMKM mKO2 GEITFEHGNVSGDMYYRMKM c c t t c a a t t g t g a a c t c a t g c c c a t t g a c g g a g c c g t c c a t g t a g t a c c t c a t c t t c a t c g g a a g t t a a c a c t t g a g t a c g g g t a a c t g c c t c g g c a g g t a c a t c a t g g a g t a g a a g t a g

End (709) EcoRVMluIPaeR7IBsoBIXhoISmlIAvaI 3ʹ 5ʹ 709 5 1 EPKIVSVM mKO2 EPKIVSVM t c t g g t t t a a t c a c a c t c a c c a t c t c g a g g t t a c g c g t t t g a t a t c a t g a g a c c a a a t t a g t g t g a g t g g t a g a g c t c c a a t g c g c a a a c t a t a g t a c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.