Addgene QUAS:GFP;betacrystallin:CFP Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: QUAS:GFP;betacrystallin:CFP Sequencing Result

Map View

800 600 400 200 End (877) PstI (826) SfcI (822) BamHI (797) BtgI - StyI - NcoI (786) BseRI (760) BanI (749) EGFP-N (723 .. 744) BtgZI (663) BsrFI (634) BssSI - BssSαI (604) BtsI - BtsαI (577) PfoI * (503) EXFP-R (464 .. 483) BmrI (184) BsiHKAI (183) EGFP-C (115 .. 136) BsrGI (75) MspA1I (55) BclI * (49) Start (0) attB3 ECFP Kozak sequence QUAS:GFP;betacrystallin:CFP 877 bp

Sequence View

MspA1IBclI*Start (0) 5ʹ 3ʹ 60 attB3 *SHIIFNDLSILPN a a c g a c g g c c a g t g a a t t a t c a a c t a t g t a t a a t a a a g t t g t c g a g g c t g a t c a g c g g g t t t g c t g c c g g t c a c t t a a t a g t t g a t a c a t a t t a t t t c a a c a g c t c c g a c t a g t c g c c c a

BsrGI 120 235 230 225 *KYLEDMGLTIGAATVF ECFP G T G C T T EGFP-C *KYLEDMGLTIGAATVF PRKVQVARGHRSHDRRRDRV t t g g c c g c t t t a c t t g t a c a g c t c g t c c a t g c c g a g a g t g a t c c c g g c g g c g g t c a c g a a a a c c g g c g a a a t g a a c a t g t c g a g c a g g t a c g g c t c t c a c t a g g g c c g c c g c c a g t g c t t

180 220 215 210 205 ELLVMHDRKENPDKSLASQT ECFP G A G G T C G T C C T G G T A C EGFP-C ELLVMHDRKENPDKSLASQT GAPGHSRAERQPRQEPRVPH c t c c a g c a g g a c c a t g t g a t c g c g c t t c t c g t t g g g g t c t t t g c t c a g g g c g g a c t g g g t g a g g t c g t c c t g g t a c a c t a g c g c g a a g a g c a a c c c c a g a a a c g a g t c c c g c c t g a c c c a

BsiHKAIBmrI 240 200 195 190 185 SLYHNDPLLVPGDGIPTNQQ ECFP SLYHNDPLLVPGDGIPTNQQ EPLPQRAAARPRRRHPHEAP g c t c a g g t a g t g g t t g t c g g g c a g c a g c a c g g g g c c g t c g c c g a t g g g g g t g t t c t g c t g c g a g t c c a t c a c c a a c a g c c c g t c g t c g t g c c c c g g c a g c g g c t a c c c c c a c a a g a c g a c

300 180 175 170 165 YHDALQVSGDEINHRIKFNA ECFP YHDALQVSGDEINHRIKFNA LPRRAARQRGRHQPPDQLQG g t a g t g g t c g g c g a g c t g c a c g c t g c c g t c c t c g a t g t t g t g g c g g a t c t t g a a g t t g g c c a t c a c c a g c c g c t c g a c g t g c g a c g g c a g g a g c t a c a a c a c c g c c t a g a a c t t c a a c c g

360 160 155 150 145 KIGNKQKDATIYVNHSIYNY ECFP KIGNKQKDATIYVNHSIYNY QHREEAQRRHYLRQPQHLQV c t t g a t g c c g t t c t t c t g c t t g t c g g c g g t g a t a t a g a c g t t g t g g c t g a t g t a g t t g t a g a a c t a c g g c a a g a a g a c g a a c a g c c g c c a c t a t a t c t g c a a c a c c g a c t a c a t c a a c a t

420 140 135 130 125 ELKHGLINGDEKFDIGKLEI ECFP ELKHGLINGDEKFDIGKLEI GAQAGPHQRGGQLRHGEARH c t c c a g c t t g t g c c c c a g g a t g t t g c c g t c c t c c t t g a a g t c g a t g c c c t t c a g c t c g a t g a g g t c g a a c a c g g g g t c c t a c a a c g g c a g g a g g a a c t t c a g c t a c g g g a a g t c g a g c t a

480 120 115 110 105 RNVLTDGEFKVEARTKYNGD ECFP G T C T T G T A G T T G C C G T C EXFP-R RNVLTDGEFKVEARTKYNGD PEGPHRGRVEGRRPDQLQRR g c g g t t c a c c a g g g t g t c g c c c t c g a a c t t c a c c t c g g c g c g g g t c t t g t a g t t g c c g t c c g c c a a g t g g t c c c a c a g c g g g a g c t t g a a g t g g a g c c g c g c c c a g a a c a t c a a c g g c a g

PfoI* 540 100 95 90 85 DKFFITREQVYGEPMASKFF ECFP G T C EXFP-R DKFFITREQVYGEPMASKFF GQLLHHAGPRLRRAHRVQLL g t c c t t g a a g a a g a t g g t g c g c t c c t g g a c g t a g c c t t c g g g c a t g g c g g a c t t g a a g a a c a g g a a c t t c t t c t a c c a c g c g a g g a c c t g c a t c g g a a g c c c g t a c c g c c t g a a c t t c t t

BtsαIBtsI 600 80 75 70 65 DHQKMHDPYRSFCQVGWTLT ECFP DHQKMHDPYRSFCQVGWTLT RAAEHPRPLPQLVARGLDPH g t c g t g c t g c t t c a t g t g g t c g g g g t a g c g g c t g a a g c a c t g c a c g c c c c a g g t c a g g g t c a g c a c g a c g a a g t a c a c c a g c c c c a t c g c c g a c t t c g t g a c g t g c g g g g t c c a g t c c c a

BsrFIBssSαIBssSI 660 60 55 50 45 TVLTPWPVPLKGTTCIFKLT ECFP TVLTPWPVPLKGTTCIFKLT DRPHALARAAQRHHLHVEPD g g t c a c g a g g g t g g g c c a g g g c a c g g g c a g c t t g c c g g t g g t g c a g a t g a a c t t c a g g g t c c a g t g c t c c c a c c c g g t c c c g t g c c c g t c g a a c g g c c a c c a c g t c t a c t t g a a g t c c c a

BtgZI 720 40 35 30 25 LKGYTADGEGEGSVSFKHGN ECFP LKGYTADGEGEGSVSFKHGN AQRLHCRGRGRRVRQVQPRK c a g c t t g c c g t a g g t g g c a t c g c c c t c g c c c t c g c c g g a c a c g c t g a a c t t g t g g c c g t t g t c g a a c g g c a t c c a c c g t a g c g g g a g c g g g a g c g g c c t g t g c g a c t t g a a c a c c g g c a a

BseRIBanI 780 20 15 10 5 VDGDLEVLIPVVGTFLEEGK ECFP C G T C G C C G T C C A G C T C G A C C A G EGFP-N VDGDLEVLIPVVGTFLEEGK RRRGARGPHAGGRHVAGRGQ t a c g t c g c c g t c c a g c t c g a c c a g g a t g g g c a c c a c c c c g g t g a a c a g c t c c t c g c c c t t a t g c a g c g g c a g g t c g a g c t g g t c c t a c c c g t g g t g g g g c c a c t t g t c g a g g a g c g g g a a

PstISfcIBamHINcoIStyIBtgI 840 S 1a V 1 M ECFP Kozak sequence SVMTAPDGLLFVSVATESLF EGHHRSGRAFICISCNRIPL g c t c a c c a t g g t g g c g g g a t c c c c t a g c a a a a a t a c a g a t a c t g c a g t t t c t g a t a g g a a c g a g t g g t a c c a c c g c c c t a g g g g a t c g t t t t t a t g t c t a t g a c g t c a a a g a c t a t c c t t

End (877) 3ʹ 5ʹ 877 TSCFLLPLFCSS NLLIAPSPFLLF g g t t g a g c a g a a t a g c a g g g g a a g g a a a c a g g a g g a a c c a a c t c g t c t t a t c g t c c c c t t c c t t t g t c c t c c t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.