Addgene mEos2-MYR-N-5 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: mEos2-MYR-N-5

Map View

1000 750 500 250 End (1233) BmgBI (1169) BtgZI (1161) TaqII (1159) PflFI - Tth111I (1146) SspI (1038) MfeI (901) DraI (856) BsaBI * (816) XbaI * (808) NotI EaeI EagI (798) NspI - SphI (755) PflMI (688) Bpu10I (651) NdeI (588) KpnI (483) Acc65I (479) BsrDI (417) Bsu36I (366) EcoRV (280) EciI (270) BaeGI - Bme1580I (177) AgeI - BsrFI (103) StyI - BtgI - NcoI (72) EcoRI (62) SacI - BsiHKAI (53) Eco53kI (51) AvaI - XhoI - BsoBI - PaeR7I (46) BglII (42) BpuEI (37) HaeII (31) AfeI (29) BmtI (28) NheI (24) AatII (16) ZraI (14) BsaHI (13) Start (0) myr mEos2 Kozak sequence mEos2-MYR-N-5 1233 bp

Sequence View

NheIBmtIAfeIHaeIIBpuEIBglIIPaeR7IBsoBIXhoIAvaIEco53kIBsiHKAISacIBsaHIZraIAatIIStart (0) 5ʹ 3ʹ 60 *AE g t c a t g c t a g t g a c g t c a g a t c c g c t a g c g c t a c c g g a c t c a g a t c t c g a g c t c a a g c t t c a g t a c g a t c a c t g c a g t c t a g g c g a t c g c g a t g g c c t g a g t c t a g a g c t c g a g t t c g a a

BsrFIAgeINcoIStyIBtgIEcoRI 120 1 5 MGSSKSK (in frame with myr) PKGPVAT 1 MS myr mEos2 Kozak sequence MGSSKSKPKGPVATMS FEGGHSAALALWLSRDGGHT c g a a t t c g c c a c c a t g g g a a g c a g c a a g a g c a a g c c a a a g g g a c c g g t c g c c a c c a t g a g g c t t a a g c g g t g g t a c c c t t c g t c g t t c t c g t t c g g t t t c c c t g g c c a g c g g t g g t a c t c

Bme1580IBaeGI 180 5 10 15 20 AIKPDMKIKLRMEGNVNGHH mEos2 AIKPDMKIKLRMEGNVNGHH RNLWVHLDFETHFAVYVPVV t g c g a t t a a g c c a g a c a t g a a g a t c a a a c t c c g t a t g g a a g g c a a c g t a a a c g g g c a c c a a c g c t a a t t c g g t c t g t a c t t c t a g t t t g a g g c a t a c c t t c c g t t g c a t t t g c c c g t g g t

240 25 30 35 40 FVIDGDGTGKPFEGKQSMDL mEos2 FVIDGDGTGKPFEGKQSMDL KHDVSITCALRKLSFLTHIK c t t t g t g a t c g a c g g a g a t g g t a c a g g c a a g c c t t t t g a g g g a a a a c a g a g t a t g g a t c t g a a a c a c t a g c t g c c t c t a c c a t g t c c g t t c g g a a a a c t c c c t t t t g t c t c a t a c c t a g a

EcoRVEciI 300 45 50 55 60 EVKEGGPLPFAFDILTTAFH mEos2 EVKEGGPLPFAFDILTTAFH FDFLASRQRKGKIDQGSCEM t g a a g t c a a a g a g g g c g g a c c t c t g c c t t t t g c c t t t g a t a t c c t g a c c a c t g c a t t c c a a c t t c a g t t t c t c c c g c c t g g a g a c g g a a a a c g g a a a c t a t a g g a c t g g t g a c g t a a g g t

360 65 70 75 80 YGNRVFAKYPDNIQDYFKQS mEos2 YGNRVFAKYPDNIQDYFKQS VAVPYEGFIWVVYLVIKLLR t t a c g g c a a c a g g g t a t t c g c c a a a t a t c c a g a c a a c a t a c a a g a c t a t t t t a a g c a g t c a a t g c c g t t g t c c c a t a a g c g g t t t a t a g g t c t g t t g t a t g t t c t g a t a a a a t t c g t c a g

BsrDIBsu36I 420 85 90 95 100 FPKGYSWERSLTFEDGGICI mEos2 FPKGYSWERSLTFEDGGICI KRLPIRPFSAQSEFVPANAN g t t t c c t a a g g g g t a t t c g t g g g a a c g a a g c t t g a c t t t c g a a g a c g g g g g c a t t t g c a t c a a a g g a t t c c c c a t a a g c a c c c t t g c t t c g a a c t g a a a g c t t c t g c c c c c g t a a a c g t a

Acc65I 480 105 110 115 120 ARNDITMEGDTFYNKVRFYG mEos2 ARNDITMEGDTFYNKVRFYG GSVVYCHFPVSEIIFNSKIT t g c c a g a a a c g a c a t a a c a a t g g a a g g g g a c a c t t t c t a t a a t a a a g t t c g a t t t t a t g g a c g g t c t t t g c t g t a t t g t t a c c t t c c c c t g t g a a a g a t a t t a t t t c a a g c t a a a a t a c c

KpnI 540 125 130 135 140 TNFPANGPVMQKKTLKWEPS mEos2 TNFPANGPVMQKKTLKWEPS GVKGGITWNHLLLRQFPLGG t a c c a a c t t t c c c g c c a a t g g t c c a g t t a t g c a g a a g a a g a c g c t g a a a t g g g a g c c c t c a t g g t t g a a a g g g c g g t t a c c a g g t c a a t a c g t c t t c t t c t g c g a c t t t a c c c t c g g g a g

NdeI 600 145 150 155 160 TEKMYVRDGVLTGDIHMALL mEos2 TEKMYVRDGVLTGDIHMALL SLFHIHTISHQRTINMHSQQ c a c t g a g a a a a t g t a t g t g c g t g a t g g a g t g c t g a c g g g t g a t a t t c a t a t g g c t t t g t t g t g a c t c t t t t a c a t a c a c g c a c t a c c t c a c g a c t g c c c a c t a t a a g t a t a c c g a a a c a a

Bpu10I 660 165 170 175 180 LEGNAHYRCDFRTTYKAKEK mEos2 LEGNAHYRCDFRTTYKAKEK KFSIGM g c t t g a a g g a a a t g c c c a t t a c c g a t g t g a c t t c a g a a c t a c t t a c a a a g c t a a g g a g a a c g a a c t t c c t t t a c g g g t a a t g g c t a c a c t g a a g t c t t g a t g a a t g t t t c g a t t c c t c t t

PflMI 720 185 190 195 200 GVKLPGYHFVDHCIEILSHD mEos2 GVKLPGYHFVDHCIEILSHD g g g t g t c a a g t t a c c a g g c t a c c a c t t t g t g g a c c a c t g c a t t g a g a t t t t a a g c c a t g a c c c a c a g t t c a a t g g t c c g a t g g t g a a a c a c c t g g t g a c g t a a c t c t a a a a t t c g g t a c t

SphINspI 780 205 210 215 220 KDYNKVKLYEHAVAHSGLPD mEos2 KDYNKVKLYEHAVAHSGLPD c a a a g a t t a c a a c a a g g t t a a g c t g t a t g a g c a t g c t g t t g c t c a t t c t g g a t t g c c t g a g t t t c t a a t g t t g t t c c a a t t c g a c a t a c t c g t a c g a c a a c g a g t a a g a c c t a a c g g a c t

BsaBI*XbaI*EagIEaeINotI 840 225 NARR* mEos2 NARR* c a a t g c c a g a c g a t a a g c g g c c g c g a c t c t a g a t c a t a a t c a g c c a t a c c a c a t t t g t a g g t t a c g g t c t g c t a t t c g c c g g c g c t g a g a t c t a g t a t t a g t c g g t a t g g t g t a a a c a t c

DraI 900 a g g t t t t a c t t g c t t t a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a c a t a a a t g a a t g t c c a a a a t g a a c g a a a t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t g t a t t t a c t t a c

MfeI 960 c a a t t g t g t g t t a c t t g t t t a t t g c a g c t a t a t g g t a c a a t a a a g c a t a g c a t c a c a a a t g t t a a c a c a c a a t g a a c a a a t a a c g t c g a t a t a c c a t g t t a t t t c g t a t c g t a g t g t t t a

1020 t c a c a a t a a g c a t t t t t t c a c t g c a t c t a g t g t g g t t g t c c a a c t c a t c a t g t a t c t t a g a g t g t t a t t c g t a a a a a a g t g a c g t a g a t c a c a c c a a c a g g t t g a g t a g t a c a t a g a a t c

SspI 1080 c g t a a a t g t a g c g t t a a t a t t t g t t a a a t c g c g t a a t t t t g t a a a t c a g c t c a t t t t a c a g c a t t t a c a t c g c a a t t a t a a a c a a t t t a g c g c a t t a a a a c a t t t a g t c g a g t a a a a t g t

1140 t a g c g a a t c g g c a a a t c c t t a a t c c a a g a a t g a c c g g a t a g g g g t g g a g t g g g t g g t c g t a t c g c t t a g c c g t t t a g g a a t t a g g t t c t t a c t g g c c t a t c c c c a c c t c a c c c a c c a g c a

BmgBITaqIIBtgZITth111IPflFI 1200 t g g a c c g a g t c c a t t a a g a c c t g a t c c a c g t c a g g c a t c g c t c t a c c g a t g c a c t t g a a c a c c t g g c t c a g g t a a t t c t g g a c t a g g t g c a g t c c g t a g c g a g a t g g c t a c g t g a a c t t g

End (1233) 3ʹ 5ʹ 1233 t g t c t t c a g t t t g g t g c a g t c c t a g c t a t c g a c a c a g a a g t c a a a c c a c g t c a g g a t c g a t a g c t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.