Addgene pCDNAIIIB cdc42 QL Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pCDNAIIIB cdc42 QL Sequencing Result

Map View

800 600 400 200 End (934) BGH-rev (783 .. 800) BclI * (776) SP6 (746 .. 763) ApaI - BaeGI - Bme1580I (740) PspOMI (736) XbaI (730) NsiI (729) SphI (727) NotI - EaeI - EagI (712) HincII (703) AccI (702) SalI (701) SmaI (698) XmaI - TspMI (696) EcoRI (691) BfuAI - PaqCI - BspMI (664) EarI - SapI - BspQI (660) SspI (634) StuI (607) AcuI (582) BsgI (574) AlwNI (537) EcoP15I (510) MspA1I (323) PstI (293) EcoRV (178) PciI - AflIII (165) MflI * - BstYI - BamHI (105) EciI (98) KpnI (97) Acc65I (93) HindIII (87) T7 (61 .. 80) AseI (60) BtsI - BtsαI (36) TsoI (10) Start (0) T7 promoter Cdc42 (Q61L) SP6 promoter bGH poly(A) signal pCDNAIIIB cdc42 QL 934 bp

Sequence View

BtsαIBtsITsoIStart (0) 5ʹ 3ʹ 60 c t a t a t a a g c a g a g c t c t c t g g c t a a c t a g a g a a c c c a c t g c t t a c t g g c t t a t c g a a a t g a t a t a t t c g t c t c g a g a g a c c g a t t g a t c t c t t g g g t g a c g a a t g a c c g a a t a g c t t t a

BamHIBstYIMflI*HindIIIAcc65IKpnIEciIAseI 120 1 M T7 promoter Cdc42 (Q61L) T A A T A C G A C T C A C T A T A G G G T7 M t a a t a c g a c t c a c t a t a g g g a g a c c c a a g c t t g g t a c c g a g c t c g g a t c c g c c a c c a t g c a t t a t g c t g a g t g a t a t c c c t c t g g g t t c g a a c c a t g g c t c g a g c c t a g g c g g t g g t a c g

EcoRVAflIIIPciI 180 5 10 15 20 QTIKCVVVGDGAVGKTCLLI Cdc42 (Q61L) QTIKCVVVGDGAVGKTCLLI a g a c a a t t a a g t g t g t t g t t g t g g g c g a t g g t g c t g t t g g t a a a a c a t g t c t c c t g a t a t t c t g t t a a t t c a c a c a a c a a c a c c c g c t a c c a c g a c a a c c a t t t t g t a c a g a g g a c t a t a

240 25 30 35 40 SYTTNKFPSEYVPTVFDNYA Cdc42 (Q61L) SYTTNKFPSEYVPTVFDNYA c c t a c a c a a c a a a c a a a t t t c c a t c g g a a t a t g t a c c g a c t g t t t t t g a c a a c t a t g c a g g g a t g t g t t g t t t g t t t a a a g g t a g c c t t a t a c a t g g c t g a c a a a a a c t g t t g a t a c g t c

PstI 300 45 50 55 60 VTVMIGGEPYTLGLFDTAG L Cdc42 (Q61L) VTVMIGGEPYTLGLFDTAGL t c a c a g t t a t g a t t g g t g g a g a a c c a t a t a c t c t t g g a c t t t t t g a t a c t g c a g g g c t c g a g t g t c a a t a c t a a c c a c c t c t t g g t a t a t g a g a a c c t g a a a a a c t a t g a c g t c c c g a g c

MspA1I 360 65 70 75 80 EDYDRLRPLSYPQTDVFLVC Cdc42 (Q61L) EDYDRLRPLSYPQTDVFLVC a g g a t t a t g a c a g a t t a c g a c c g c t g a g t t a t c c a c a a a c a g a t g t a t t t c t a g t c t g t t t c c t a a t a c t g t c t a a t g c t g g c g a c t c a a t a g g t g t t t g t c t a c a t a a a g a t c a g a c a a

420 85 90 95 100 FSVVSPSSFENVKEKWVPEI Cdc42 (Q61L) FSVVSPSSFENVKEKWVPEI t t t c a g t g g t c t c t c c a t c t t c a t t t g a a a a c g t g a a a g a a a a g t g g g t g c c t g a g a t a a a a a g t c a c c a g a g a g g t a g a a g t a a a c t t t t g c a c t t t c t t t t c a c c c a c g g a c t c t a t t

480 105 110 115 120 THHCPKTPFLLVGTQIDLRD Cdc42 (Q61L) THHCPKTPFLLVGTQIDLRD c t c a c c a c t g t c c a a a g a c t c c t t t c t t g c t t g t t g g g a c t c a a a t t g a t c t c a g a g a t g g a g t g g t g a c a g g t t t c t g a g g a a a g a a c g a a c a a c c c t g a g t t t a a c t a g a g t c t c t a c

AlwNIEcoP15I 540 125 130 135 140 DPSTIEKLAKNKQKPITPET Cdc42 (Q61L) DPSTIEKLAKNKQKPITPET a c c c c t c t a c t a t t g a g a a a c t t g c c a a g a a c a a a c a g a a g c c t a t c a c t c c a g a g a c t g t g g g g a g a t g a t a a c t c t t t g a a c g g t t c t t g t t t g t c t t c g g a t a g t g a g g t c t c t g a c

AcuIBsgI 600 145 150 155 160 AEKLARDLKAVKYVECSALT Cdc42 (Q61L) AEKLARDLKAVKYVECSALT c t g a a a a g c t g g c c c g t g a c c t g a a g g c t g t c a a g t a t g t g g a g t g t t c t g c a c t t a c a c g a c t t t t c g a c c g g g c a c t g g a c t t c c g a c a g t t c a t a c a c c t c a c a a g a c g t g a a t g t g

SspIStuI 660 165 170 175 180 QKGLKNVFDEAILAALEPPE Cdc42 (Q61L) QKGLKNVFDEAILAALEPPE a g a a a g g c c t a a a g a a t g t a t t t g a c g a a g c a a t a t t g g c t g c c c t g g a g c c t c c a g a a c t c t t t c c g g a t t t c t t a c a t a a a c t g c t t c g t t a t a a c c g a c g g g a c c t c g g a g g t c t t g

EagIEaeINotIEcoRITspMIXmaISmaISalIAccIHincIIBspQISapIEarIBspMIPaqCIBfuAI 720 185 190 PKKSRRCVLL* Cdc42 (Q61L) PKKSRRCVLL* c g a a g a a g a g c c g c a g g t g t g t g c t g c t a t g a a t t c c c g g g t c g a c t c g a g c g g c c g c t c g c t t c t t c t c g g c g t c c a c a c a c g a c g a t a c t t a a g g g c c c a g c t g a g c t c g c c g g c g a g

BclI*SphINsiIXbaIPspOMIBme1580IBaeGIApaI 780 SP6 promoter G A T A T C A C A G T G G A T T T A SP6 g a g c a t g c a t c t a g a g g g c c c t a t t c t a t a g t g t c a c c t a a a t g c t a g a g c t c g c t g a t c c t c g t a c g t a g a t c t c c c g g g a t a a g a t a t c a c a g t g g a t t t a c g a t c t c g a g c g a c t a g

840 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g

900 bGH poly(A) signal c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g

End (934) 3ʹ 5ʹ 934 g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.