Addgene MXS_CMV::rtTA3-bGHpA - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: MXS_CMV::rtTA3-bGHpA


Map View

EcoRV (6) PciI (3449) DrdI (3347) pBR322ori-F (3294 .. 3313) HaeII (3209) AlwNI (3040) AhdI (2561) BsaI (2495) FspI (2338) PvuI (2192) EaeI (2168) TsoI (2163) ScaI (2080) Amp-R (1992 .. 2011) XmnI (1961) EarI (1762) MluI (12) PaeR7I - PspXI - XhoI (20) SnaBI (365) NcoI - StyI (385) CMV-F (544 .. 564) PvuII (629) ApoI - EcoRI (646) XbaI (658) XcmI (660) Eco53kI (691) SacI (693) StuI (725) PmlI (786) PspOMI (800) EcoO109I (801) ApaI (804) BseRI (913) NruI (935) MfeI (1172) TspMI - XmaI (1393) SmaI (1395) BbsI (1611) SphI (1622) SalI (1646) AccI (1647) HincII (1648) SspI (1756) MXS_CMV::rtTA3-bGHpA 3561 bp

Sequence View

PaeR7IPspXIXhoIMluIEcoRV 60 c a t g a t a t c a a a c g c g t a a c t c g a g t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g g t a c t a t a g t t t g c g c a t t g a g c t c a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c

120 CMV enhancer t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a

180 CMV enhancer g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g

240 CMV enhancer c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c

300 CMV enhancer c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a

360 CMV enhancer g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t

NcoIStyISnaBI 420 CMV enhancer CMV promoter t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g

480 CMV promoter g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t

540 CMV promoter g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a

600 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c

XbaIXcmIEcoRIApoIPvuII 660 1 rTetR MS rtTA-Advanced MS t g a a c c g t c a g a t c c g c t a g g g a t c t c a g c t g g t c g a g g c a g g t c g a a t t c a c c a t g t c t a c t t g g c a g t c t a g g c g a t c c c t a g a g t c g a c c a g c t c c g t c c a g c t t a a g t g g t a c a g a

Eco53kISacI 720 5 10 15 20 rTetR RLDKSKVINGALELLNGVGI rtTA-Advanced RLDKSKVINGALELLNGVGI a g a c t g g a c a a g a g c a a a g t c a t a a a c g g a g c t c t g g a a t t a c t c a a t g g t g t c g g t a t c t c t g a c c t g t t c t c g t t t c a g t a t t t g c c t c g a g a c c t t a a t g a g t t a c c a c a g c c a t a g

StuI 780 25 30 35 40 rTetR EGLTTRKLAQKLGVEQPTLY rtTA-Advanced EGLTTRKLAQKLGVEQPTLY g a a g g c c t g a c g a c a a g g a a a c t c g c t c a a a a g c t g g g a g t t g a g c a g c c t a c c c t g t a c c t t c c g g a c t g c t g t t c c t t t g a g c g a g t t t t c g a c c c t c a a c t c g t c g g a t g g g a c a t g

PspOMIEcoO109IApaIPmlI 840 45 50 55 60 rTetR WHVKNKRALLDALPIEMLDR rtTA-Advanced WHVKNKRALLDALPIEMLDR t g g c a c g t g a a g a a c a a g c g g g c c c t g c t c g a t g c c c t g c c a a t c g a g a t g c t g g a c a g g a c c g t g c a c t t c t t g t t c g c c c g g g a c g a g c t a c g g g a c g g t t a g c t c t a c g a c c t g t c c

900 65 70 75 80 rTetR HHTHFCPLEGESWQDFLRNN rtTA-Advanced HHTHFCPLEGESWQDFLRNN c a t c a t a c c c a c t t c t g c c c c c t g g a a g g c g a g t c a t g g c a a g a c t t t c t g c g g a a c a a c g t a g t a t g g g t g a a g a c g g g g g a c c t t c c g c t c a g t a c c g t t c t g a a a g a c g c c t t g t t g

NruIBseRI 960 85 90 95 100 rTetR AKSYRCALLSHRDGAKVHLG rtTA-Advanced AKSYRCALLSHRDGAKVHLG g c c a a g t c a t a c c g c t g t g c t c t c c t c t c a c a t c g c g a c g g g g c t a a a g t g c a t c t c g g c c g g t t c a g t a t g g c g a c a c g a g a g g a g a g t g t a g c g c t g c c c c g a t t t c a c g t a g a g c c g

1020 105 110 115 120 rTetR TRPTEKQYETLENQLAFLCQ rtTA-Advanced TRPTEKQYETLENQLAFLCQ a c c c g c c c a a c a g a g a a a c a g t a c g a a a c c c t g g a a a a t c a g c t c g c g t t c c t g t g t c a g t g g g c g g g t t g t c t c t t t g t c a t g c t t t g g g a c c t t t t a g t c g a g c g c a a g g a c a c a g t c

1080 125 130 135 140 rTetR QGFSLENALYALSAVGHFTL rtTA-Advanced QGFSLENALYALSAVGHFTL c a a g g c t t c t c c c t g g a g a a c g c a c t g t a c g c t c t g t c c g c c g t g g g c c a c t t t a c a c t g g t t c c g a a g a g g g a c c t c t t g c g t g a c a t g c g a g a c a g g c g g c a c c c g g t g a a a t g t g a c

1140 145 150 155 160 rTetR GCVLEEQEHQVAKEERETPT rtTA-Advanced GCVLEEQEHQVAKEERETPT g g c t g c g t a t t g g a g g a a c a g g a g c a t c a a g t a g c a a a a g a g g a a a g a g a g a c a c c t a c c c c g a c g c a t a a c c t c c t t g t c c t c g t a g t t c a t c g t t t t c t c c t t t c t c t c t g t g g a t g g

MfeI 1200 165 170 175 180 rTetR TDSMPPLLRQAIELFDRQGA rtTA-Advanced TDSMPPLLRQAIELFDRQGA a c c g a t t c t a t g c c c c c a c t t c t g a g a c a a g c a a t t g a g c t g t t c g a c c g g c a g g g a g c c t g g c t a a g a t a c g g g g g t g a a g a c t c t g t t c g t t a a c t c g a c a a g c t g g c c g t c c c t c g g

1260 185 190 195 200 rTetR EPAFLFGLELIICGLEKQLK rtTA-Advanced EPAFLFGLELIICGLEKQLK g a a c c t g c c t t c c t t t t c g g c c t g g a a c t a a t c a t a t g t g g c c t g g a g a a a c a g c t a a a g c t t g g a c g g a a g g a a a a g c c g g a c c t t g a t t a g t a t a c a c c g g a c c t c t t t g t c g a t t t c

1320 205 rTetR CESG GP 210 215 220 VP16 minimal transcriptional activation domain TDALDDFDLDML P VP... A rtTA-Advanced CESGGPTDALDDFDLDMLPA t g c g a a a g c g g c g g g c c g a c c g a c g c c c t t g a c g a t t t t g a c t t a g a c a t g c t c c c a g c c a c g c t t t c g c c g c c c g g c t g g c t g c g g g a a c t g c t a a a a c t g a a t c t g t a c g a g g g t c g g

1380 225 230 VP16 minimal transcriptional activation domain DALDDFDLDML P 235 240 VP16 minimal transcriptional activation domain ADALDDFD rtTA-Advanced DALDDFDLDMLPADALDDFD g a t g c c c t t g a c g a c t t t g a c c t t g a t a t g c t g c c t g c t g a c g c t c t t g a c g a t t t t g a c c t a c g g g a a c t g c t g a a a c t g g a a c t a t a c g a c g g a c g a c t g c g a g a a c t g c t a a a a c t g

TspMIXmaISmaI 1440 245 VP16 minimal transcri... LDML PG* rtTA-Advanced bGH poly(A) signal LDMLPG* c t t g a c a t g c t c c c c g g g t a a c t a a g t a a g g a t c t g t c g a g c t g t g c c t t c t a g t t g c c a g a a c t g t a c g a g g g g c c c a t t g a t t c a t t c c t a g a c a g c t c g a c a c g g a a g a t c a a c g g t

1500 bGH poly(A) signal g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g

1560 bGH poly(A) signal t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a

BbsI 1620 bGH poly(A) signal t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t

SalIAccIHincIISphI 1680 bGH poly(A) signal AmpR promoter t g c t g g g g a t g c g g t g g g c t c t a t g g t c g a c c a a t t c g g g g a a a t g t g c g c g g a a c c c c t a c g a c c c c t a c g c c a c c c g a g a t a c c a g c t g g t t a a g c c c c t t t a c a c g c g c c t t g g g g a

1740 AmpR promoter a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t

EarISspI 1800 1 5 signal sequence MSIQHFRVA AmpR promoter AmpR MSIQHFRVA t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g

1860 10 15 20 signal sequence LIPFFAAFCLPVFA 25 HPETLV AmpR LIPFFAAFCLPVFAHPETLV c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c

1920 30 35 40 45 KVKDAEDQLGARVGYIELDL AmpR KVKDAEDQLGARVGYIELDL a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g

XmnI 1980 50 55 60 65 NSGKILESFRPEERFPMMST AmpR NSGKILESFRPEERFPMMST a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a

2040 70 75 80 85 FKVLLCGAVLSRIDAGQEQL AmpR C G A T A C A C C G C G C C A T A A T A Amp-R FKVLLCGAVLSRIDAGQEQL t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g

ScaI 2100 90 95 100 105 GRRIHYSQNDLVEYSPVTEK AmpR GRRIHYSQNDLVEYSPVTEK g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c

2160 110 115 120 125 HLTDGMTVRELCSAAITMSD AmpR HLTDGMTVRELCSAAITMSD c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a

PvuIEaeITsoI 2220 130 135 140 145 NTAANLLLTTIGGPKELTAF AmpR NTAANLLLTTIGGPKELTAF a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a

2280 150 155 160 165 LHNMGDHVTRLDRWEPELNE AmpR LHNMGDHVTRLDRWEPELNE t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t

FspI 2340 170 175 180 185 AIPNDERDTTMPVAMATTLR AmpR AIPNDERDTTMPVAMATTLR g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g

2400 190 195 200 205 KLLTGELLTLASRQQLIDWM AmpR KLLTGELLTLASRQQLIDWM a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c

2460 210 215 220 225 EADKVAGPLLRSALPAGWFI AmpR EADKVAGPLLRSALPAGWFI g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a

BsaI 2520 230 235 240 245 ADKSGAGERGSRGIIAALGP AmpR ADKSGAGERGSRGIIAALGP g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t

AhdI 2580 250 255 260 265 DGKPSRIVVIYTTGSQATMD AmpR DGKPSRIVVIYTTGSQATMD g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a

2640 270 275 280 285 ERNRQIAEIGASLIKHW* AmpR ERNRQIAEIGASLIKHW* g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t

2700 g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c

2760 a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c

2820 ori t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a

2880 ori c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c

2940 ori c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t

3000 ori c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t

AlwNI 3060 ori c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c

3120 ori t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g

3180 ori t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t

HaeII 3240 ori t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c

3300 ori G G G A A A C pBR322ori-F t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g

DrdI 3360 ori G C C T G G T A T C T T T pBR322ori-F g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c

3420 ori t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c

PciI 3480 t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a

3540 g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g

3561 g a g c g c a g c g a g t c a g t g a g c c t c g c g t c g c t c a g t c a c t c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.