Addgene pCI-MOR30-1 - Sequence Analyzer Skip to main content
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Sequence Analyzer: pCI-MOR30-1

Map View

BglII (4982) pBR322ori-F (4815 .. 4834) AlwNI (4561) AhdI (4082) NmeAIII (3935) Amp-R (3513 .. 3532) XmnI (3482) pBRforEco (3104 .. 3122) pGEX 3' (3044 .. 3066) pRS-marker (2925 .. 2944) F1ori-F (2700 .. 2721) NaeI (2530) NgoMIV (2528) F1ori-R (2490 .. 2509) BsrGI (96) SpeI (152) SnaBI (493) CMV-F (672 .. 692) LNCX (718 .. 742) Eco53kI (719) SacI (721) PstI (830) BfuAI - BspMI (844) BbsI (928) pMT2-F (972 .. 991) T7 promoter NheI (1052) BmtI (1056) EcoRI (1121) MluI (1127) BspQI - SapI (1159) PasI (1190) CsiI - SexAI * (1347) BseRI (1351) PflMI (1392) EcoNI (1433) PvuII (1577) BbvCI - Bpu10I (1814) Bsu36I (1825) PciI (1856) PmlI (1939) EagI - NotI (2079) EBV-rev (2112 .. 2131) SV40pA-R (2166 .. 2185) HpaI (2222) MfeI (2231) BspDI - ClaI (2317) BsaBI * (2323) BamHI (2324) pCI-MOR30-1 4987 bp

Sequence View

5ʹ 3ʹ 60 t c a a t a t t g g c c a t t a g c c a t a t t a t t c a t t g g t t a t a t a g c a t a a a t c a a t a t t g g c t a a g t t a t a a c c g g t a a t c g g t a t a a t a a g t a a c c a a t a t a t c g t a t t t a g t t a t a a c c g a t

BsrGI 120 t t g g c c a t t g c a t a c g t t g t a t c t a t a t c a t a a t a t g t a c a t t t a t a t t g g c t c a t g t c c a a c c g g t a a c g t a t g c a a c a t a g a t a t a g t a t t a t a c a t g t a a a t a t a a c c g a g t a c a g g

SpeI 180 CMV enhancer a a t a t g a c c g c c a t g t t g g c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g t t a t a c t g g c g g t a c a a c c g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c

240 CMV enhancer g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g

300 CMV enhancer g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a

360 CMV enhancer a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g

420 CMV enhancer c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t c c g c c c c c t a t t g a c g t c a a t g a g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a g g c g g g g g a t a a c t g c a g t t a c t

480 CMV enhancer c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a c g g g a c t t t c c t a c t t g g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t g c c c t g a a a g g a t g a a c

SnaBI 540 CMV enhancer CMV promoter g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t g

600 CMV promoter c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a

660 CMV promoter c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a t a a c c c g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t a t t g g g

Eco53kI 720 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F A G C LNCX c g c c c c g t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c g c g g g g c a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g

SacI 780 CMV promoter T C G T T T A G T G A A C C G T C A G A T C LNCX t c g t t t a g t g a a c c g t c a g a t c a c t a g a a g c t t t a t t g c g g t a g t t t a t c a c a g t t a a a t a g c a a a t c a c t t g g c a g t c t a g t g a t c t t c g a a a t a a c g c c a t c a a a t a g t g t c a a t t t a

PstI 840 t g c t a a c g c a g t c a g t g c t t c t g a c a c a a c a g t c t c g a a c t t a a g c t g c a g a a g t t g g t c a c g a t t g c g t c a g t c a c g a a g a c t g t g t t g t c a g a g c t t g a a t t c g a c g t c t t c a a c c a g

BspMIBfuAI 900 chimeric intron g t g a g g c a c t g g g c a g g t a a g t a t c a a g g t t a c a a g a c a g g t t t a a g g a g a c c a a t a g a a c a c t c c g t g a c c c g t c c a t t c a t a g t t c c a a t g t t c t g t c c a a a t t c c t c t g g t t a t c t t

BbsI 960 chimeric intron a c t g g g c t t g t c g a g a c a g a g a a g a c t c t t g c g t t t c t g a t a g g c a c c t a t t g g t c t t a c t g a c c c g a a c a g c t c t g t c t c t t c t g a g a a c g c a a a g a c t a t c c g t g g a t a a c c a g a a t g

1020 chimeric intron T T G C C T T T C T C T C C A C A G G T pMT2-F t g a c a t c c a c t t t g c c t t t c t c t c c a c a g g t g t c c a c t c c c a g t t c a a t t a c a g c t c t t a a c t g t a g g t g a a a c g g a a a g a g a g g t g t c c a c a g g t g a g g g t c a a g t t a a t g t c g a g a a t

BmtINheI 1080 T7 promoter MNGTEGPN a g g c t a g a g t a c t t a a t a c g a c t c a c t a t a g g c t a g c a t g a a t g g c a c a g a a g g c c c t a a t c c g a t c t c a t g a a t t a t g c t g a g t g a t a t c c g a t c g t a c t t a c c g t g t c t t c c g g g a t t

MluIEcoRI 1140 FYVPFSNATGVVREFTRMVA c t t c t a c g t g c c c t t c t c c a a t g c g a c g g g t g t g g t a c g c g a a t t c a c g c g t a t g g t g g c g a a g a t g c a c g g g a a g a g g t t a c g c t g c c c a c a c c a t g c g c t t a a g t g c g c a t a c c a c c g

PasIBspQISapI 1200 SSNSSSHPLFFMLLGIPGLE t t c a a g t a a c a g c t c t t c c c a t c c c c t g t t c t t c a t g t t g c t t g g a a t c c c a g g g t t g g a a a g t t c a t t g t c g a g a a g g g t a g g g g a c a a g a a g t a c a a c g a a c c t t a g g g t c c c a a c c t

1260 NYQFWIAFPFCVMYIVALTG g a a t t a t c a a t t t t g g a t t g c c t t t c c t t t c t g t g t t a t g t a t a t t g t g g c a c t g a c t g g c t t a a t a g t t a a a a c c t a a c g g a a a g g a a a g a c a c a a t a c a t a t a a c a c c g t g a c t g a c c

1320 NITILYIIRIDHTLHEPMYL a a a t a t c a c c a t c c t a t a c a t a a t c c g a a t t g a c c a c a c c c t g c a t g a g c c c a t g t a c c t t t t a t a g t g g t a g g a t a t g t a t t a g g c t t a a c t g g t g t g g g a c g t a c t c g g g t a c a t g g a

SexAI*CsiIBseRI 1380 FLALLAITDLVLSSSTQPKM c t t c c t g g c c t t g t t g g c t a t c a c t g a c c t g g t c c t g t c c t c c t c c a c a c a a c c t a a a a t g a a g g a c c g g a a c a a c c g a t a g t g a c t g g a c c a g g a c a g g a g g a g g t g t g t t g g a t t t t a

EcoNIPflMI 1440 LAILWFHSHEIEYNACLIQV g c t g g c c a t a c t a t g g t t t c a c a g t c a t g a g a t t g a a t a c a a c g c c t g c c t c a t c c a g g t c g a c c g g t a t g a t a c c a a a g t g t c a g t a c t c t a a c t t a t g t t g c g g a c g g a g t a g g t c c a

1500 FFIHAFSSVESGVLMTMALD g t t c t t c a t a c a t g c c t t t t c t t c t g t g g a g t c t g g g g t g c t c a t g a c c a t g g c c t t g g a c a a g a a g t a t g t a c g g a a a a g a a g a c a c c t c a g a c c c c a c g a g t a c t g g t a c c g g a a c c t

1560 RYVAICFPLRHSSILTTSVV c c g c t a t g t g g c t a t c t g c t t c c c a c t c c g a c a t t c c a g c a t c c t g a c c a c a t c t g t a g t g g c g a t a c a c c g a t a g a c g a a g g g t g a g g c t g t a a g g t c g t a g g a c t g g t g t a g a c a t c a

PvuII 1620 IKLGAAVMVRGLLWVSPFCF c a t t a a a c t a g g g g c a g c t g t g a t g g t g a g a g g g c t g c t g t g g g t g a g c c c c t t c t g c t t g t a a t t t g a t c c c c g t c g a c a c t a c c a c t c t c c c g a c g a c a c c c a c t c g g g g a a g a c g a a

1680 MVSRMPFCPNKVIPQSYCEH t a t g g t c t c c a g g a t g c c c t t c t g c c c c a a c a a g g t c a t t c c c c a g t c c t a c t g t g a g c a a t a c c a g a g g t c c t a c g g g a a g a c g g g g t t g t t c c a g t a a g g g g t c a g g a t g a c a c t c g t

1740 MAVLKLVCADTRVNRGYGLF c a t g g c t g t g c t c a a g t t g g t a t g t g c t g a t a c c a g a g t c a a t c g t g g a t a t g g a c t c t t g t a c c g a c a c g a g t t c a a c c a t a c a c g a c t a t g g t c t c a g t t a g c a c c t a t a c c t g a g a a

1800 VAFSVVGFDIIVISVSYVMI t g t g g c t t t c t c t g t g g t t g g c t t t g a c a t a a t t g t c a t c a g t g t a t c t t a t g t g a t g a t a c a c c g a a a g a g a c a c c a a c c g a a a c t g t a t t a a c a g t a g t c a c a t a g a a t a c a c t a c t a

PciIBsu36IBpu10IBbvCI 1860 LRAVLRLPSGEARLKAFGTC t c t g a g a g c t g t g c t g a g g t t g c c c t c a g g t g a a g c t c g c c t c a a a g c t t t t g g t a c a t g a g a c t c t c g a c a c g a c t c c a a c g g g a g t c c a c t t c g a g c g g a g t t t c g a a a a c c a t g t a c

1920 ASHVCVILAFYIPALFTFLT t g c t t c t c a t g t c t g t g t t a t t t t g g c t t t c t a t a t c c c a g c c c t t t t t a c c t t c c t c a c a c g a a g a g t a c a g a c a c a a t a a a a c c g a a a g a t a t a g g g t c g g g a a a a a t g g a a g g a g t g

PmlI 1980 HRFGHHVPRVVHIMFANFYL c c a c c g c t t t g g c c a c c a c g t g c c a c g a g t t g t c c a t a t c a t g t t t g c t a a t t t c t a t c t g g t g g c g a a a c c g g t g g t g c a c g g t g c t c a a c a g g t a t a g t a c a a a c g a t t a a a g a t a g a

2040 LVPPMLNPIIYGVRTKQIRD g c t t g t t c c t c c c a t g c t c a a c c c t a t t a t c t a t g g a g t t a g a a c c a a a c a g a t c a g g g a c g a a c a a g g a g g g t a c g a g t t g g g a t a a t a g a t a c c t c a a t c t t g g t t t g t c t a g t c c c t

EagINotI 2100 RVIRGFRRKDP* c a g g g t c a t c c g a g g a t t c a g g a g a a a a g a c c c t t g a g c g g c c g c t t c g a g c a g a c a t g a g t c c c a g t a g g c t c c t a a g t c c t c t t t t c t g g g a a c t c g c c g g c g a a g c t c g t c t g t a c t

2160 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t t a a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a a t

2220 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a g a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t c

MfeIHpaI 2280 SV40 poly(A) signal t t a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g a t g t g g g a g g t t t a a t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c t a c a c c c t c c a a a

BspDIClaIBsaBI*BamHI 2340 t t t a a a g c a a g t a a a a c c t c t a c a a a t g t g g t a a a a t c g a t a a g g a t c c g g g c t g g c g t a a a a t t t c g t t c a t t t t g g a g a t g t t t a c a c c a t t t t a g c t a t t c c t a g g c c c g a c c g c a t

2400 a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a

2460 f1 ori g g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g

2520 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g

NgoMIVNaeI 2580 f1 ori c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a

2640 f1 ori t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c

2700 f1 ori G F1ori-F g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c

2760 f1 ori T G G A C T C T T G T T C C A A A C T G G F1ori-F t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a

2820 f1 ori a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a

2880 f1 ori t a a c g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t c c t g a t g c g g t a t t t t c t c c t t a t t g c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a g g a c t a c g c c a t a a a a g a g g a a

2940 A T G T T A G A C G A G A C T A pRS-marker a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a

3000 C G G C pRS-marker g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t c g g c g t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a

3060 C C G G G A G C T G C A T G T G T pGEX 3' t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a

3120 C A G A G G pGEX 3' C G G A G C A C T A T G C G G A T pBRforEco c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g a c g a a a g g g c c t c g t g a t a c g c c t a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c t g c t t t c c c g g a g c a c t a t g c g g a t

3180 A A pBRforEco t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c a c t t t t c g g a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g t g a a a a g c c

3240 AmpR promoter g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c

3300 1 signal se... MS AmpR promoter AmpR MS c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a

3360 5 10 15 20 signal sequence IQHFRVALIPFFAAFCLPVF AmpR IQHFRVALIPFFAAFCLPVF a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a

3420 sig... A 25 30 35 40 HPETLVKVKDAEDQLGARV AmpR AHPETLVKVKDAEDQLGARV g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c

3480 45 50 55 60 GYIELDLNSGKILESFRPEE AmpR GYIELDLNSGKILESFRPEE g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t

XmnI 3540 65 70 75 80 RFPMMSTFKVLLCGAVLSRI AmpR C G A T A C A C C G C G C C A T A A T A Amp-R RFPMMSTFKVLLCGAVLSRI c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a

3600 85 90 95 100 DAGQEQLGRRIHYSQNDLVE AmpR DAGQEQLGRRIHYSQNDLVE g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c

3660 105 110 115 120 YSPVTEKHLTDGMTVRELCS AmpR YSPVTEKHLTDGMTVRELCS t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a

3720 125 130 135 140 AAITMSDNTAANLLLTTIGG AmpR AAITMSDNTAANLLLTTIGG g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t

3780 145 150 155 160 PKELTAFLHNMGDHVTRLDR AmpR PKELTAFLHNMGDHVTRLDR c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a

3840 165 170 175 180 WEPELNEAIPNDERDTTMPV AmpR WEPELNEAIPNDERDTTMPV t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t

3900 185 190 195 200 AMATTLRKLLTGELLTLASR AmpR AMATTLRKLLTGELLTLASR g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c

NmeAIII 3960 205 210 215 220 QQLIDWMEADKVAGPLLRSA AmpR QQLIDWMEADKVAGPLLRSA c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g

4020 225 230 235 240 LPAGWFIADKSGAGERGSRG AmpR LPAGWFIADKSGAGERGSRG c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a

4080 245 250 255 260 IIAALGPDGKPSRIVVIYTT AmpR IIAALGPDGKPSRIVVIYTT a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c

AhdI 4140 265 270 275 280 GSQATMDERNRQIAEIGASL AmpR GSQATMDERNRQIAEIGASL g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c

4200 285 IKHW* AmpR IKHW* a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t

4260 c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t

4320 a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t

4380 ori t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c

4440 ori c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a

4500 ori g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g

4560 ori c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c

AlwNI 4620 ori g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c

4680 ori g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t

4740 ori a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g

4800 ori g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c

4860 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g

4920 ori t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g

4980 a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g g c t c g a t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c c g a g c t

BglII 3ʹ 5ʹ 4987 c a g a t c t g t c t a g a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.