Addgene pCI-OR51E2 - Sequence Analyzer Skip to main content
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Sequence Analyzer: pCI-OR51E2


Map View

BglII (4997) pBR322ori-F (4830 .. 4849) PspFI (4685) BseYI (4681) AlwNI (4576) AhdI (4097) BpmI (4028) NmeAIII (3950) Amp-R (3528 .. 3547) XmnI (3497) pBRforEco (3119 .. 3137) pGEX 3' (3059 .. 3081) PfoI (3058) pRS-marker (2940 .. 2959) F1ori-F (2715 .. 2736) DraIII (2651) NaeI (2545) NgoMIV (2543) SpeI (152) CMV enhancer SnaBI (493) CMV-F (672 .. 692) LNCX (718 .. 742) Eco53kI (719) SacI (721) HindIII (748) pMT2-F (972 .. 991) T7 promoter NheI (1052) BmtI (1056) EcoRI (1121) AflIII - MluI (1127) BsgI (1277) SphI (1324) AvaI - BsoBI (1395) SacII (1582) BclI * (1614) PflMI (1895) PvuII (2058) EagI - NotI (2094) EBV-rev (2127 .. 2146) SV40pA-R (2181 .. 2200) HpaI (2237) MfeI (2246) BspDI - ClaI (2332) F1ori-R (2505 .. 2524) pCI-OR51E2 5002 bp

Sequence View

60 t c a a t a t t g g c c a t t a g c c a t a t t a t t c a t t g g t t a t a t a g c a t a a a t c a a t a t t g g c t a a g t t a t a a c c g g t a a t c g g t a t a a t a a g t a a c c a a t a t a t c g t a t t t a g t t a t a a c c g a t

120 t t g g c c a t t g c a t a c g t t g t a t c t a t a t c a t a a t a t g t a c a t t t a t a t t g g c t c a t g t c c a a c c g g t a a c g t a t g c a a c a t a g a t a t a g t a t t a t a c a t g t a a a t a t a a c c g a g t a c a g g

SpeI 180 CMV enhancer a a t a t g a c c g c c a t g t t g g c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g t t a t a c t g g c g g t a c a a c c g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c

240 CMV enhancer g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g

300 CMV enhancer g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a

360 CMV enhancer a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g

420 CMV enhancer c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t c c g c c c c c t a t t g a c g t c a a t g a g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a g g c g g g g g a t a a c t g c a g t t a c t

480 CMV enhancer c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a c g g g a c t t t c c t a c t t g g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t g c c c t g a a a g g a t g a a c

SnaBI 540 CMV enhancer CMV promoter g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t g

600 CMV promoter c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a

660 CMV promoter c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a t a a c c c g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t a t t g g g

Eco53kI 720 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F A G C LNCX c g c c c c g t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c g c g g g g c a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g

HindIIISacI 780 CMV promoter T C G T T T A G T G A A C C G T C A G A T C LNCX t c g t t t a g t g a a c c g t c a g a t c a c t a g a a g c t t t a t t g c g g t a g t t t a t c a c a g t t a a a t a g c a a a t c a c t t g g c a g t c t a g t g a t c t t c g a a a t a a c g c c a t c a a a t a g t g t c a a t t t a

840 t g c t a a c g c a g t c a g t g c t t c t g a c a c a a c a g t c t c g a a c t t a a g c t g c a g a a g t t g g t c a c g a t t g c g t c a g t c a c g a a g a c t g t g t t g t c a g a g c t t g a a t t c g a c g t c t t c a a c c a g

900 chimeric intron g t g a g g c a c t g g g c a g g t a a g t a t c a a g g t t a c a a g a c a g g t t t a a g g a g a c c a a t a g a a c a c t c c g t g a c c c g t c c a t t c a t a g t t c c a a t g t t c t g t c c a a a t t c c t c t g g t t a t c t t

960 chimeric intron a c t g g g c t t g t c g a g a c a g a g a a g a c t c t t g c g t t t c t g a t a g g c a c c t a t t g g t c t t a c t g a c c c g a a c a g c t c t g t c t c t t c t g a g a a c g c a a a g a c t a t c c g t g g a t a a c c a g a a t g

1020 chimeric intron T T G C C T T T C T C T C C A C A G G T pMT2-F t g a c a t c c a c t t t g c c t t t c t c t c c a c a g g t g t c c a c t c c c a g t t c a a t t a c a g c t c t t a a c t g t a g g t g a a a c g g a a a g a g a g g t g t c c a c a g g t g a g g g t c a a g t t a a t g t c g a g a a t

BmtINheI 1080 T7 promoter MNGTEGPN a g g c t a g a g t a c t t a a t a c g a c t c a c t a t a g g c t a g c a t g a a t g g c a c a g a a g g c c c t a a t c c g a t c t c a t g a a t t a t g c t g a g t g a t a t c c g a t c g t a c t t a c c g t g t c t t c c g g g a t t

AflIIIMluIEcoRI 1140 FYVPFSNATGVVREFTRMSS c t t c t a c g t g c c c t t c t c c a a t g c g a c g g g t g t g g t a c g c g a a t t c a c g c g t a t g a g t t c g a a g a t g c a c g g g a a g a g g t t a c g c t g c c c a c a c c a t g c g c t t a a g t g c g c a t a c t c a a g

1200 CNFTHATFVLIGIPGLEKAH c t g c a a c t t c a c a c a t g c c a c c t t t g t g c t t a t t g g t a t c c c a g g a t t a g a g a a a g c c c a g a c g t t g a a g t g t g t a c g g t g g a a a c a c g a a t a a c c a t a g g g t c c t a a t c t c t t t c g g g t

1260 FWVGFPLLSMYVVAMFGNCI t t t c t g g g t t g g c t t c c c c c t c c t t t c c a t g t a t g t a g t g g c a a t g t t t g g a a a c t g c a t a a a g a c c c a a c c g a a g g g g g a g g a a a g g t a c a t a c a t c a c c g t t a c a a a c c t t t g a c g t a

BsgI 1320 VVFIVRTERSLHAPMYLFLC c g t g g t c t t c a t c g t a a g g a c g g a a c g c a g c c t g c a c g c t c c g a t g t a c c t c t t t c t c t g g c a c c a g a a g t a g c a t t c c t g c c t t g c g t c g g a c g t g c g a g g c t a c a t g g a g a a a g a g a c

SphI 1380 MLAAIDLALSTSTMPKILAL c a t g c t t g c a g c c a t t g a c c t g g c c t t a t c c a c a t c c a c c a t g c c t a a g a t c c t t g c c c t g t a c g a a c g t c g g t a a c t g g a c c g g a a t a g g t g t a g g t g g t a c g g a t t c t a g g a a c g g g a

BsoBIAvaI 1440 FWFDSREISFEACLTQMFFI t t t c t g g t t t g a t t c c c g a g a g a t t a g c t t t g a g g c c t g t c t t a c c c a g a t g t t c t t t a t a a a g a c c a a a c t a a g g g c t c t c t a a t c g a a a c t c c g g a c a g a a t g g g t c t a c a a g a a a t a

1500 HALSAIESTILLAMAFDRYV t c a t g c c c t c t c a g c c a t t g a a t c c a c c a t c c t g c t g g c c a t g g c c t t t g a c c g t t a t g t a g t a c g g g a g a g t c g g t a a c t t a g g t g g t a g g a c g a c c g g t a c c g g a a a c t g g c a a t a c a

1560 AICHPLRHAAVLNNTVTAQI g g c c a t c t g c c a c c c a c t g c g c c a t g c t g c a g t g c t c a a c a a t a c a g t a a c a g c c c a g a t c c g g t a g a c g g t g g g t g a c g c g g t a c g a c g t c a c g a g t t g t t a t g t c a t t g t c g g g t c t a

BclI*SacII 1620 GIVAVVRGSLFFFPLPLLIK t g g c a t c g t g g c t g t g g t c c g c g g a t c c c t c t t t t t t t t c c c a c t g c c t c t g c t g a t c a a a c c g t a g c a c c g a c a c c a g g c g c c t a g g g a g a a a a a a a a g g g t g a c g g a g a c g a c t a g t t

1680 RLAFCHSNVLSHSYCVHQDV g c g g c t g g c c t t c t g c c a c t c c a a t g t c c t c t c a c a c t c c t a t t g t g t c c a c c a g g a t g t c g c c g a c c g g a a g a c g g t g a g g t t a c a g g a g a g t g t g a g g a t a a c a c a g g t g g t c c t a c a

1740 MKLAYADTLPNVVYGLTAIL a a t g a a g t t g g c c t a t g c a g a c a c t t t g c c c a a t g t g g t a t a t g g t c t t a c t g c c a t t c t t t a c t t c a a c c g g a t a c g t c t g t g a a a c g g g t t a c a c c a t a t a c c a g a a t g a c g g t a a g a

1800 LVMGVDVMFISLSYFLIIRT g c t g g t c a t g g g t g t g g a c g t a a t g t t c a t c t c c t t g t c c t a t t t t c t g a t a a t a c g a a c c g a c c a g t a c c c a c a c c t g c a t t a c a a g t a g a g g a a c a g g a t a a a a g a c t a t t a t g c t t g

1860 VLQLPSKSERAKAFGTCVSH g g t t c t g c a a c t g c c t t c c a a g t c a g a g c g g g c c a a g g c c t t t g g a a c c t g t g t g t c a c a c c a a g a c g t t g a c g g a a g g t t c a g t c t c g c c c g g t t c c g g a a a c c t t g g a c a c a c a g t g t

PflMI 1920 IGVVLAFYVPLIGLSVVHRF c a t t g g t g t g g t a c t c g c c t t c t a t g t g c c a c t t a t t g g c c t c t c a g t t g t a c a c c g c t t g t a a c c a c a c c a t g a g c g g a a g a t a c a c g g t g a a t a a c c g g a g a g t c a a c a t g t g g c g a a

1980 GNSLHPIVRVVMGDIYLLLP t g g a a a c a g c c t t c a t c c c a t t g t g c g t g t t g t c a t g g g t g a c a t c t a c c t g c t g c t g c c a c c t t t g t c g g a a g t a g g g t a a c a c g c a c a a c a g t a c c c a c t g t a g a t g g a c g a c g a c g g

2040 PVINPIIYGAKTKQIRTRVL t c c t g t c a t c a a t c c c a t c a t c t a t g g t g c c a a a a c c a a a c a g a t c a g a a c a c g g g t g c t a g g a c a g t a g t t a g g g t a g t a g a t a c c a c g g t t t t g g t t t g t c t a g t c t t g t g c c c a c g a

EagINotIPvuII 2100 AMFKISCDKDLQAVGGKAAA g g c t a t g t t c a a g a t c a g c t g t g a c a a g g a c t t g c a g g c t g t g g g a g g c a a g g c g g c c g c c c g a t a c a a g t t c t a g t c g a c a c t g t t c c t g a a c g t c c g a c a c c c t c c g t t c c g c c g g c g

2160 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev SSRHDKIH* t t c g a g c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t a a g c t c g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a

2220 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t

MfeIHpaI 2280 SV40 poly(A) signal g c t g c a a t a a a c a a g t t a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g g g c g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c c c

BspDIClaI 2340 a g a t g t g g g a g g t t t t t t a a a g c a a g t a a a a c c t c t a c a a a t g t g g t a a a a t c g a t a a g g t c t a c a c c c t c c a a a a a a t t t c g t t c a t t t t g g a g a t g t t t a c a c c a t t t t a g c t a t t c c

2400 a t c c g g g c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a t a g g c c c g a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t

2460 f1 ori g c c t g a a t g g c g a a t g g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t c g g a c t t a c c g c t t a c c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a

2520 f1 ori G A G G A A A G C G A A A G A A F1ori-R t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a

NgoMIVNaeI 2580 f1 ori G G G A F1ori-R c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g

2640 f1 ori t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t

DraIII 2700 f1 ori t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g

2760 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a

2820 f1 ori c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a

2880 f1 ori g a t t t a a c a a a a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t c c t g a t g c t a a a t t g t t t t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a g g a c t a c

2940 A pRS-marker c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a t g g t g c a c t c t c a g t g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t a c c a c g t g a g a g t c a

3000 T G T T A G A C G A G A C T A C G G C pRS-marker a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a c t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t g

PfoI 3060 C C pGEX 3' g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g

3120 G G G A G C T G C A T G T G T C A G A G G pGEX 3' C G pBRforEco g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g a c g a a a g g g c c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c t g c t t t c c c g

3180 G A G C A C T A T G C G G A T A A pBRforEco c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g t c a g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c a g t

3240 AmpR promoter g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a

3300 AmpR promoter t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t

3360 1 5 10 15 signal sequence MSIQHFRVALIPFFAAF AmpR promoter AmpR MSIQHFRVALIPFFAAF a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a

3420 20 signal sequence CLPVFA 25 30 35 HPETLVKVKDAEDQ AmpR CLPVFAHPETLVKVKDAEDQ t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c

3480 40 45 50 55 LGARVGYIELDLNSGKILES AmpR LGARVGYIELDLNSGKILES t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a

XmnI 3540 60 65 70 75 FRPEERFPMMSTFKVLLCGA AmpR C G A T A C A C C G C G C Amp-R FRPEERFPMMSTFKVLLCGA t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c

3600 80 85 90 95 VLSRIDAGQEQLGRRIHYSQ AmpR C A T A A T A Amp-R VLSRIDAGQEQLGRRIHYSQ g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c

3660 100 105 110 115 NDLVEYSPVTEKHLTDGMTV AmpR NDLVEYSPVTEKHLTDGMTV a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t

3720 120 125 130 135 RELCSAAITMSDNTAANLLL AmpR RELCSAAITMSDNTAANLLL a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c

3780 140 145 150 155 TTIGGPKELTAFLHNMGDHV AmpR TTIGGPKELTAFLHNMGDHV a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t

3840 160 165 170 175 TRLDRWEPELNEAIPNDERD AmpR TRLDRWEPELNEAIPNDERD a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g

3900 180 185 190 195 TTMPVAMATTLRKLLTGELL AmpR TTMPVAMATTLRKLLTGELL a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a

NmeAIII 3960 200 205 210 215 TLASRQQLIDWMEADKVAGP AmpR TLASRQQLIDWMEADKVAGP a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t

4020 220 225 230 235 LLRSALPAGWFIADKSGAGE AmpR LLRSALPAGWFIADKSGAGE c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c

BpmI 4080 240 245 250 255 RGSRGIIAALGPDGKPSRIV AmpR RGSRGIIAALGPDGKPSRIV c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t

AhdI 4140 260 265 270 275 VIYTTGSQATMDERNRQIAE AmpR VIYTTGSQATMDERNRQIAE g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c

4200 280 285 IGASLIKHW* AmpR IGASLIKHW* a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a

4260 t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a

4320 a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t

4380 ori g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t

4440 ori a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a

4500 ori t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c

4560 ori c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t

AlwNI 4620 ori a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t

4680 ori a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c

BseYIPspFI 4740 ori c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t

4800 ori a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t

4860 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g

4920 ori g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g

4980 ori c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a

BglII 5002 g c t c a c a t g g c t c g a c a g a t c t c g a g t g t a c c g a g c t g t c t a g a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.