Addgene pQFlag-USP11 WT puroR Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pQFlag-USP11 WT puroR Sequencing Result


Map View

600 400 200 End (756) FspI (747) BmrI (651) MscI (595) XbaI (584) CsiI - SexAI * (565) PspFI (549) BseYI (545) EcoNI (507) SmlI (504) BpuEI (489) BsgI (441) BpmI - Eco57MI (425) BsaAI (419) NaeI (365) NgoMIV (363) BseRI (303) StuI * (301) BssSI - BssSαI (285) BtgZI (213) EarI - SfcI (179) BbsI (144) BspEI (128) PfoI (115) BanII Bsp1286I (103) AgeI (66) MfeI (36) StyI - NcoI (28) NotI - EagI - SacII (17) Start (0) FLAG pQFlag-USP11 WT puroR 756 bp

Sequence View

MfeINcoIStyISacIIEagINotIStart (0) 60 1 5 DYKDDD FLAG QELIRGRDTMAQLDYKDDD LFQDAAAVGHCLQIVLIVI t g c a g g a a t t g a t c c g c g g c c g c g a c a c c a t g g c a c a a t t g g a t t a c a a g g a t g a c g a t g a c g t c c t t a a c t a g g c g c c g g c g c t g t g g t a c c g t g t t a a c c t a a t g t t c c t a c t g c t a c

PfoIBsp1286IBanIIAgeI 120 DK (in frame with FLAG) TGMAVAPRLFGGLCFRFR FLAG DKTGMAVAPRLFGGLCFRFR VLGTHCYGRSQKPPEAETEP a c a a g a c c g g t a t g g c a g t a g c c c c g c g a c t g t t t g g g g g g c t c t g c t t c c g t t t c c g g g t g t t c t g g c c a t a c c g t c a t c g g g g c g c t g a c a a a c c c c c c g a g a c g a a g g c a a a g g c c c

EarISfcIBbsIBspEI 180 (in frame with FLAG) DQNPEVAVEGRLPISHSCVG DQNPEVAVEGRLPISHSCVG VLIRFHSNLPTKWDRVAANA a c c a g a a t c c g g a a g t g g c t g t t g a g g g g c g t c t t c c a a t c t c g c a c a g c t g c g t t g g c t t g g t c t t a g g c c t t c a c c g a c a a c t c c c c g c a g a a g g t t a g a g c g t g t c g a c g c a a c c g a

BtgZI 240 (in frame with FLAG) CRRERTAMATVAANPAAAAA CRRERTAMATVAANPAAAAA TSSFPRRHRRDCCIWSSSRR g t a g a a g a g a a c g g a c g g c g a t g g c g a c g g t c g c a g c a a a t c c a g c t g c t g c t g c g g c g g c a t c t t c t c t t g c c t g c c g c t a c c g c t g c c a g c g t c g t t t a g g t c g a c g a c g a c g c c g c c

BssSαIBssSI 300 (in frame with FLAG) AVAAAAAVTEDREPQHEELP AVAAAAAVTEDREPQHEELP SHRRCRRHSLISLWLVLLQW c t g t g g c g g c g g c a g c g g c g g t g a c t g a g g a t a g a g a g c c a c a g c a c g a g g a g c t g c c a g g a c a c c g c c g c c g t c g c c g c c a c t g a c t c c t a t c t c t c g g t g t c g t g c t c c t c g a c g g t c

StuI*BseRI 360 (in frame with FLAG) GLDSQWRQIENGESGRERPL GLDSQWRQIENGESGRERPL AQVALPALYFVALTPSFTWQ g c c t g g a c a g c c a g t g g c g c c a g a t a g a a a a c g g c g a g a g t g g g c g a g a a c g t c c a c t g c c g g a c c t g t c g g t c a c c g c g g t c t a t c t t t t g c c g c t c t c a c c c g c t c t t g c a g g t g a c g

BsaAINgoMIVNaeI 420 (in frame with FLAG) RAGESWFLVEKHWYKQWEAY RAGESWFLVEKHWYKQWEAY PGAFAPEKHLLVPILLPLCV g g g c c g g c g a a a g c t g g t t c c t t g t g g a g a a g c a c t g g t a t a a g c a g t g g g a g g c a t a c g c c c g g c c g c t t t c g a c c a a g g a a c a c c t c t t c g t g a c c a t a t t c g t c a c c c t c c g t a t g c

BsgIEco57MIBpmI 480 (in frame with FLAG) VQGGDQDSSTFPGCINNATL VQGGDQDSSTFPGCINNATL HLSPVLVGAGERAADVIGCE t g c a g g g a g g g g a c c a g g a c t c c a g c a c c t t c c c t g g c t g c a t c a a c a a t g c c a c a c t c t a c g t c c c t c c c c t g g t c c t g a g g t c g t g g a a g g g a c c g a c g t a g t t g t t a c g g t g t g a g a

SmlIEcoNIBpuEI 540 (in frame with FLAG) FQDEINWRLKEGLVEGEDYV FQDEINWRLKEGLVEGEDYV KLILYVPAELLSQHFALIIH t t c a a g a t g a g a t a a a c t g g c g c c t c a a g g a g g g a c t g g t g g a a g g c g a g g a t t a t g t g c a a g t t c t a c t c t a t t t g a c c g c g g a g t t c c t c c c t g a c c a c c t t c c g c t c c t a a t a c a c g

MscIXbaISexAI*CsiIBseYIPspFI 600 (in frame with FLAG) LLPAAAWHYLVSWYGLEHGQ LLPAAAWHYLVSWYGLEHGQ QEWCSSPM t g c t c c c a g c a g c t g c t t g g c a t t a c c t g g t c a g c t g g t a t g g t c t a g a g c a t g g c c a g c a c g a g g g t c g t c g a c g a a c c g t a a t g g a c c a g t c g a c c a t a c c a g a t c t c g t a c c g g t c g

BmrI 660 (in frame with FLAG) PPIERKVIELPNIQKVEVYP PPIERKVIELPNIQKVEVYP c a c c c a t t g a a c g c a a g g t c a t a g a g c t g c c c a a c a t c c a g a a g g t c g a a g t g t a c c c a g g t g g g t a a c t t g c g t t c c a g t a t c t c g a c g g g t t g t a g g t c t t c c a g c t t c a c a t g g g t c

720 (in frame with FLAG) VELLLVRHNDLGKSHTVQFS VELLLVRHNDLGKSHTVQFS t a g a a c t g c t g c t t g t c c g g c a c a a t g a t t t g g g c a a a t c t c a c a c t g t t c a g t t c a g c c a t c t t g a c g a c g a a c a g g c c g t g t t a c t a a a c c c g t t t a g a g t g t g a c a a g t c a a g t c g g

End (756) FspI 756 (in frame with FLAG) HTDSIGLVLRTA HTDSIGLVLRTA a t a c c g a t t c t a t t g g c c t a g t a t t g c g c a c a g c t c t a t g g c t a a g a t a a c c g g a t c a t a a c g c g t g t c g a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.