Addgene C1A-C1A-EYFP Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: C1A-C1A-EYFP Sequencing Result

Map View

600 400 200 End (728) BsrFI (690) BssSI - BssSαI (660) BtsI - BtsαI (633) PfoI * (559) EXFP-R (520 .. 539) MflI * - BstYI (341) EGFP-C (171 .. 192) BsrGI (131) BsiEI (122) NotI - EagI (119) XbaI * (109) BsaBI * (105) DraI (65) BsmI (18) MfeI (13) HpaI - HincII (4) Start (0) VC155 C1A-C1A-EYFP 728 bp

Sequence View

BsmIMfeIStart (0) HincIIHpaI 5ʹ 3ʹ 60 a g t t a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g g t g t g g g a g g t t c a a t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c c a c a c c c t c c a

EagINotIBsaBI*XbaI*DraI 120 *SRSDR t t t t t a a a g c a a g t a a a a c c t c t a c a a a t g t g g t a t g g c t g a t t a t g a t c t a g a g t c g c g a a a a a t t t c g t t c a t t t t g g a g a t g t t t a c a c c a t a c c g a c t a a t a c t a g a t c t c a g c g c

BsrGIBsiEI 180 * 235 230 225 KYLEDMGLTIGAATVFE VC155 G T G C T T G A G G EGFP-C GSVQVARGHRSHDRRRDRVG *KYLEDMGLTIGAATVFE g c c g c t t a c t t g t a c a g c t c g t c c a t g c c g a g a g t g a t c c c g g c g g c g g t c a c g a a c t c c c g g c g a a t g a a c a t g t c g a g c a g g t a c g g c t c t c a c t a g g g c c g c c g c c a g t g c t t g a g g

240 220 215 210 205 LLVMHDRKENPDKSLASQYS VC155 T C G T C C T G G T A C EGFP-C APGHSRAERQPRQEPRVPLE LLVMHDRKENPDKSLASQYS a g c a g g a c c a t g t g a t c g c g c t t c t c g t t g g g g t c t t t g c t c a g g g c g g a c t g g t a g c t c t c g t c c t g g t a c a c t a g c g c g a a g a g c a a c c c c a g a a a c g a g t c c c g c c t g a c c a t c g a g

300 200 195 190 185 LYHNDPLLVPGDGIPTNQQY VC155 PLPQRAAARPRRRHPHEAPL LYHNDPLLVPGDGIPTNQQY a g g t a g t g g t t g t c g g g c a g c a g c a c g g g g c c g t c g c c g a t g g g g g t g t t c t g c t g g t a g t c c a t c a c c a a c a g c c c g t c g t c g t g c c c c g g c a g c g g c t a c c c c c a c a a g a c g a c c a t c

BstYIMflI* 360 180 175 170 165 HDALQVSGDEINHRIKFNVK VC155 PRRAARQRGRHQPPDQLEGQ HDALQVSGDEINHRIKFNVK t g g t c g g c g a g c t g c a c g c t g c c g t c c t c g a t g t t g t g g c g g a t c t t g a a g t t c a c c t t g a c c a g c c g c t c g a c g t g c g a c g g c a g g a g c t a c a a c a c c g c c t a g a a c t t c a a g t g g a a c

420 160 155 IGNKQKD VC155 HREEAQRGHYLRQPQQLQVG IGNKQKDAMIYVNHSNYNYE a t g c c g t t c t t c t g c t t g t c g g c c a t g a t a t a g a c g t t g t g g c t g t t g t a g t t g t a c t c c t a c g g c a a g a a g a c g a a c a g c c g g t a c t a t a t c t g c a a c a c c g a c a a c a t c a a c a t g a g g

480 AQAGPHQRGGQLRHGEARHP LKHGLINGDEKFDIGKLEIR a g c t t g t g c c c c a g g a t g t t g c c g t c c t c c t t g a a g t c g a t g c c c t t c a g c t c g a t g c g g t c g a a c a c g g g g t c c t a c a a c g g c a g g a g g a a c t t c a g c t a c g g g a a g t c g a g c t a c g c c

540 G T C T T G T A G T T G C C G T C G T C EXFP-R EGPHRGRVEGRRPDQLQRRG NVLTDGEFKVEARTKYNGDD t t c a c c a g g g t g t c g c c c t c g a a c t t c a c c t c g g c g c g g g t c t t g t a g t t g c c g t c g t c c a a g t g g t c c c a c a g c g g g a g c t t g a a g t g g a g c c g c g c c c a g a a c a t c a a c g g c a g c a g g

PfoI* 600 QLLHHAGPRLRRAHRVQLLR KFFITREQVYGEPMASKFFD t t g a a g a a g a t g g t g c g c t c c t g g a c g t a g c c t t c g g g c a t g g c g g a c t t g a a g a a g t c g a a c t t c t t c t a c c a c g c g a g g a c c t g c a t c g g a a g c c c g t a c c g c c t g a a c t t c t t c a g c

BtsαIBtsI 660 AAEHPRPLPRLVARRLRLHD HQKMHDPYRAFCQVGYGFTT t g c t g c t t c a t g t g g t c g g g g t a g c g g g c g a a g c a c t g c a c g c c g t a g c c g a a g g t g g t c a c g a c g a a g t a c a c c a g c c c c a t c g c c c g c t t c g t g a c g t g c g g c a t c g g c t t c c a c c a g

BsrFIBssSαIBssSI 720 RPHALARAAQRHHLHVEPDA VLTPWPVPLKGTTCIFKLTL a c g a g g g t g g g c c a g g g c a c g g g c a g c t t g c c g g t g g t g c a g a t g a a c t t c a g g g t c a g c t g c t c c c a c c c g g t c c c g t g c c c g t c g a a c g g c c a c c a c g t c t a c t t g a a g t c c c a g t c g

End (728) 3ʹ 5ʹ 728 QR KGY t t g c c g t a a a c g g c a t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.