A general system for generating unlabelled gene replacements in bacterial chromosomes.
Leenhouts K, Buist G, Bolhuis A, ten Berge A, Kiel J, Mierau I, Dabrowska M, Venema G, Kok J
Mol Gen Genet. 1996 Nov 27;253(1-2):217-24. PubMed
Leenhouts K, Buist G, Bolhuis A, ten Berge A, Kiel J, Mierau I, Dabrowska M, Venema G, Kok J
Mol Gen Genet. 1996 Nov 27;253(1-2):217-24. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71595 | pORI28 | EC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria. |
| 71852 | EC1000 | RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids |